MX368315B - Derivación de células madre neurales y neuronas dopaminérgicas a partir de células madre pluripotentes humanas. - Google Patents

Derivación de células madre neurales y neuronas dopaminérgicas a partir de células madre pluripotentes humanas.

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Publication number
MX368315B
MX368315B MX2014013054A MX2014013054A MX368315B MX 368315 B MX368315 B MX 368315B MX 2014013054 A MX2014013054 A MX 2014013054A MX 2014013054 A MX2014013054 A MX 2014013054A MX 368315 B MX368315 B MX 368315B
Authority
MX
Mexico
Prior art keywords
stem cells
human pluripotent
derivation
dopaminergic neurons
pluripotent stem
Prior art date
Application number
MX2014013054A
Other languages
English (en)
Other versions
MX2014013054A (es
Inventor
Gonzalez Rodolfo
Garitaonandia Ibon
MECHKIN Ruslan
Original Assignee
Int Stem Cell Corporation
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Filing date
Publication date
Application filed by Int Stem Cell Corporation filed Critical Int Stem Cell Corporation
Publication of MX2014013054A publication Critical patent/MX2014013054A/es
Publication of MX368315B publication Critical patent/MX368315B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Neurosurgery (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un método definido desde el punto de vista químico para generar células madre neurales (NSC) y neuronas dopaminérgicas (DA) a partit de células madre pluripotentes humanas (hPSC). Las neuronas DA de la invención pueden derivar de las hPSC y las NSC. La presente invención también proporciona reactivos y kits útiles para la derivación de las células madre neurales y neuronas dopaminérgicas de células madre pluripotentes humanas.
MX2014013054A 2012-04-24 2013-04-23 Derivación de células madre neurales y neuronas dopaminérgicas a partir de células madre pluripotentes humanas. MX368315B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261637797P 2012-04-24 2012-04-24
US201261637784P 2012-04-24 2012-04-24
PCT/US2013/037860 WO2013163228A1 (en) 2012-04-24 2013-04-23 Derivation of neural stem cells and dopaminergic neurons from human pluripotent stem cells

Publications (2)

Publication Number Publication Date
MX2014013054A MX2014013054A (es) 2016-12-20
MX368315B true MX368315B (es) 2019-09-27

Family

ID=49483836

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014013054A MX368315B (es) 2012-04-24 2013-04-23 Derivación de células madre neurales y neuronas dopaminérgicas a partir de células madre pluripotentes humanas.

Country Status (13)

Country Link
US (1) US9926529B2 (es)
EP (1) EP2841566B1 (es)
JP (1) JP6262205B2 (es)
KR (1) KR20150004834A (es)
CN (1) CN104379732B (es)
AU (1) AU2013251749B2 (es)
CA (1) CA2871337C (es)
HK (1) HK1201873A1 (es)
IL (1) IL235109B (es)
MX (1) MX368315B (es)
RU (1) RU2014147015A (es)
SG (2) SG10201608838QA (es)
WO (1) WO2013163228A1 (es)

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US8642334B2 (en) 2009-02-17 2014-02-04 Memorial Sloan Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
AU2012332269B2 (en) 2011-11-04 2018-05-31 Memorial Sloan-Kettering Cancer Center Midbrain dopamine (DA) neurons for engraftment
EP2961277A4 (en) 2013-02-15 2016-12-28 Int Stem Cell Corp USE OF NEURONS FROM HUMAN PLURIPOTENT STEM CELLS FOR THE TREATMENT OF NEURODEGENERATIVE DISEASES
WO2014176606A1 (en) 2013-04-26 2014-10-30 Memorial Sloan-Kettering Center Center Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells
US10444227B2 (en) 2014-08-19 2019-10-15 FUJIFILM Cellular Dynamics, Inc. Neural networks formed from cells derived from pluripotent stem cells
EP3313420B1 (en) 2015-06-25 2024-03-13 The Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion, enrichment, and maintenance
LU92845B1 (en) 2015-10-08 2017-05-02 Univ Du Luxembourg Campus Belval Means and methods for generating midbrain organoids
EP3430132A4 (en) * 2016-03-14 2019-12-25 Agency for Science, Technology and Research GENERATION OF SPECIES-SPECIFIC ORGANOIDS FROM MENENCEPHALUS FROM HUMAN PLURIPOTENT STEM CELLS
EP4049665A1 (en) 2016-03-15 2022-08-31 Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion
JP2019517257A (ja) 2016-05-31 2019-06-24 ザ ジェイ． デヴィッド グラッドストーン インスティテューツ 幹細胞をニューロンに分化するインビトロ法、及びこの方法を用いて生成されたニューロン
WO2019051248A1 (en) * 2017-09-07 2019-03-14 Memorial Sloan-Kettering Cancer Center METHODS FOR DIFFERENTIATING PRECURSORS OF ECTODERMIC LINES DERIVED FROM STEM CELLS
JP2019106895A (ja) 2017-12-15 2019-07-04 インダストリー‐ユニバーシティー コーぺレーション ファンデーション ハンヤン ユニバーシティ 神経幹細胞または神経前駆細胞からドーパミン神経細胞への分化方法
CN108559726A (zh) * 2017-12-28 2018-09-21 上海安集协康生物技术股份有限公司 一种对脑损伤类疾病有治疗作用的细胞亚群及其制备方法
KR102119148B1 (ko) * 2018-03-08 2020-06-04 고려대학교 산학협력단 신경조직 오가노이드의 제조방법, 이를 이용한 신경조직 형성 억제 물질의 스크리닝 방법 및 운동신경세포의 제조방법
US20220233602A1 (en) * 2019-07-05 2022-07-28 Novo Nordisk A/S Generation of neural stem cell lines derived from human pluripotent stem cells
CN110432888B (zh) * 2019-08-15 2021-11-23 任鹏宇 一种前庭神经权重信息编码神经元的筛选方法
AU2020350408A1 (en) * 2019-09-20 2022-04-07 Korea Research Institute Of Bioscience And Biotechnology Production method for induced dopaminergic neuronal progenitors, using direct reprogramming
EP4061929A1 (en) * 2019-11-22 2022-09-28 Novo Nordisk A/S Spin-aggregated neural microspheres and the application thereof
JP2021084882A (ja) * 2019-11-28 2021-06-03 学校法人慶應義塾 脊髄損傷治療用ニューロスフェア誘導剤及びその使用
CN111269887B (zh) * 2020-02-28 2021-11-16 上海市东方医院（同济大学附属东方医院） 一种人多能干细胞向外胚层分化的方法
US20230027059A1 (en) * 2021-07-19 2023-01-26 Trallhead Biosystems Inc. Methods and compositions for generating human midbrain neural progenitor cells
AU2022344320A1 (en) 2021-09-10 2024-03-07 FUJIFILM Cellular Dynamics, Inc. Compositions of induced pluripotent stem cell-derived cells and methods of use thereof
AU2022376962A1 (en) 2021-10-29 2024-04-18 FUJIFILM Cellular Dynamics, Inc. Dopaminergic neurons comprising mutations and methods of use thereof
CN114292807A (zh) * 2021-12-28 2022-04-08 深圳市夏同生物医药科技有限公司 一种外泌体的提取方法及其应用
CN115521903B (zh) * 2022-09-30 2024-05-17 中国科学院生态环境研究中心 体外诱导人多能干细胞分化为多巴胺能神经元的方法

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Also Published As

Publication number Publication date
US20150087541A1 (en) 2015-03-26
IL235109A0 (en) 2014-12-31
SG10201608838QA (en) 2016-12-29
JP6262205B2 (ja) 2018-01-17
CN104379732A (zh) 2015-02-25
EP2841566B1 (en) 2019-03-13
CN104379732B (zh) 2018-08-17
HK1201873A1 (en) 2015-09-11
US9926529B2 (en) 2018-03-27
RU2014147015A (ru) 2016-06-10
IL235109B (en) 2020-05-31
EP2841566A1 (en) 2015-03-04
JP2015514437A (ja) 2015-05-21
WO2013163228A1 (en) 2013-10-31
CA2871337A1 (en) 2013-10-31
EP2841566A4 (en) 2015-12-23
AU2013251749A1 (en) 2014-11-13
MX2014013054A (es) 2016-12-20
SG11201406825WA (en) 2014-11-27
CA2871337C (en) 2021-04-20
KR20150004834A (ko) 2015-01-13
AU2013251749B2 (en) 2019-01-17

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