MX2022008764A - Cromatografia de interaccion hidrofobica para la eliminacion viral. - Google Patents

Cromatografia de interaccion hidrofobica para la eliminacion viral.

Info

Publication number
MX2022008764A
MX2022008764A MX2022008764A MX2022008764A MX2022008764A MX 2022008764 A MX2022008764 A MX 2022008764A MX 2022008764 A MX2022008764 A MX 2022008764A MX 2022008764 A MX2022008764 A MX 2022008764A MX 2022008764 A MX2022008764 A MX 2022008764A
Authority
MX
Mexico
Prior art keywords
viral clearance
hic
interaction chromatography
hydrophobic interaction
column
Prior art date
Application number
MX2022008764A
Other languages
English (en)
Inventor
John Mattila
Robert Stairs
John Ruppino
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2022008764A publication Critical patent/MX2022008764A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39516—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum from serum, plasma
    • A61K39/39525—Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente solicitud proporciona un método para caracterizar y/o determinar capacidad de eliminación viral de cromatografía de interacción hidrofóbica (HIC) que incluye un diseño experimental para análisis multivariante de eliminación viral por HIC. El método proporciona la comprensión del mecanismo de la eliminación viral mediante el uso de HIC al ejecutar un diseño D-Óptimo de experimento que incluye evaluaciones de múltiples factores, tal como pH, concentración de solución amortiguadora, concentración de carga de columna, velocidad de flujo de la columna o fuerza hidrofóbica de la columna HIC.
MX2022008764A 2020-01-17 2021-01-15 Cromatografia de interaccion hidrofobica para la eliminacion viral. MX2022008764A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202062962506P 2020-01-17 2020-01-17
PCT/US2021/013739 WO2021146630A1 (en) 2020-01-17 2021-01-15 Hydrophobic interaction chromatography for viral clearance

Publications (1)

Publication Number Publication Date
MX2022008764A true MX2022008764A (es) 2022-07-27

Family

ID=74626149

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022008764A MX2022008764A (es) 2020-01-17 2021-01-15 Cromatografia de interaccion hidrofobica para la eliminacion viral.

Country Status (11)

Country Link
US (1) US20210221840A1 (es)
EP (1) EP4090668A1 (es)
JP (1) JP2023510595A (es)
KR (1) KR20220129032A (es)
CN (1) CN115003683A (es)
AU (1) AU2021208632A1 (es)
BR (1) BR112022013624A2 (es)
CA (1) CA3167669A1 (es)
IL (1) IL294677A (es)
MX (1) MX2022008764A (es)
WO (1) WO2021146630A1 (es)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140071452A (ko) * 2006-04-05 2014-06-11 애브비 바이오테크놀로지 리미티드 항체 정제
AU2009307737B2 (en) * 2008-10-20 2015-07-23 Abbvie Inc. Viral inactivation during purification of antibodies
CA2775595A1 (en) * 2009-10-20 2011-04-28 Abbott Laboratories Isolation and purification of anti-il-13 antibodies using protein a affinity chromatography
SG189872A1 (en) * 2010-10-11 2013-06-28 Abbvie Inc Processes for purification of proteins
WO2016118707A1 (en) * 2015-01-21 2016-07-28 Oncobiologics, Inc. Modulation of charge variants in a monoclonal antibody composition

Also Published As

Publication number Publication date
CN115003683A (zh) 2022-09-02
KR20220129032A (ko) 2022-09-22
JP2023510595A (ja) 2023-03-14
CA3167669A1 (en) 2021-07-22
AU2021208632A1 (en) 2022-08-04
US20210221840A1 (en) 2021-07-22
BR112022013624A2 (pt) 2022-09-13
WO2021146630A1 (en) 2021-07-22
EP4090668A1 (en) 2022-11-23
IL294677A (en) 2022-09-01

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