MX2021003768A - Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra. - Google Patents

Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra.

Info

Publication number
MX2021003768A
MX2021003768A MX2021003768A MX2021003768A MX2021003768A MX 2021003768 A MX2021003768 A MX 2021003768A MX 2021003768 A MX2021003768 A MX 2021003768A MX 2021003768 A MX2021003768 A MX 2021003768A MX 2021003768 A MX2021003768 A MX 2021003768A
Authority
MX
Mexico
Prior art keywords
compositions
barcodes
capture probe
indexes
arrays
Prior art date
Application number
MX2021003768A
Other languages
English (en)
Inventor
Lorenzo Berti
Darren Segale
Fiona E Black
Jeffrey Dennis Brodin
Jeffrey Fisher
Siew Hong Leong
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2021003768A publication Critical patent/MX2021003768A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/101—Primer extension
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Algunas modalidades proporcionadas en la presente descripción incluyen métodos y composiciones para la detección de ligandos objetivo en un arreglo. En algunas modalidades, una sonda de captura se une específicamente a un ligando objetivo de una muestra, se determina la ubicación de un glóbulo que comprende la sonda de captura en un arreglo, y el glóbulo se decodifica para identificar la sonda de captura y la muestra. En algunas modalidades, un código de barras es indicativo de una sonda de captura unida a un glóbulo; y un índice es indicativo de una subpoblación de glóbulos. En algunas modalidades, el código de barras y el índice se determinan por secuenciación.
MX2021003768A 2018-10-25 2019-10-23 Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra. MX2021003768A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862750370P 2018-10-25 2018-10-25
US201962903108P 2019-09-20 2019-09-20
PCT/US2019/057704 WO2020086746A1 (en) 2018-10-25 2019-10-23 Methods and compositions for identifying ligands on arrays using indexes and barcodes

Publications (1)

Publication Number Publication Date
MX2021003768A true MX2021003768A (es) 2021-05-27

Family

ID=70328366

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021003768A MX2021003768A (es) 2018-10-25 2019-10-23 Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra.

Country Status (12)

Country Link
US (2) US11667957B2 (es)
EP (1) EP3870704A4 (es)
JP (1) JP2022503873A (es)
KR (1) KR20210084441A (es)
CN (2) CN117778535A (es)
AU (1) AU2019364418A1 (es)
BR (1) BR112021006190A2 (es)
CA (1) CA3113270A1 (es)
IL (1) IL281551A (es)
MX (1) MX2021003768A (es)
SG (1) SG11202102722SA (es)
WO (1) WO2020086746A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210087613A1 (en) 2019-09-20 2021-03-25 Illumina, Inc. Methods and compositions for identifying ligands on arrays using indexes and barcodes
WO2023096675A1 (en) * 2021-11-23 2023-06-01 Pleno, Inc. Encoded endonuclease assays
CN113416766B (zh) * 2021-07-02 2022-08-19 苏州拉索生物芯片科技有限公司 一种生物芯片的解码方法
CN116536399A (zh) * 2023-07-07 2023-08-04 之江实验室 一种基于微珠测序的基因芯片编解码方法及基因芯片

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
EP2327797B1 (en) 1997-04-01 2015-11-25 Illumina Cambridge Limited Method of nucleic acid sequencing
EP2045334A1 (en) * 1998-06-24 2009-04-08 Illumina, Inc. Decoding of array sensors with microspheres
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
AU2004205311A1 (en) * 1999-04-20 2004-09-23 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
WO2003002979A2 (en) * 2001-06-28 2003-01-09 Illumina, Inc. Multiplex decoding of array sensors with microspheres
JP4480715B2 (ja) 2003-01-29 2010-06-16 ４５４ コーポレーション 二重末端シーケンシング
WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
DE102008061774A1 (de) * 2008-12-11 2010-06-17 Febit Holding Gmbh Indexierung von Nukleinsäure-Populationen
RU2609630C2 (ru) * 2009-02-27 2017-02-02 Конинклейке Филипс Электроникс Н.В. Геномный отбор и секвенирование с помощью кодированных микроносителей
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
SG10201805453XA (en) * 2013-04-25 2018-08-30 Firefly Bioworks Inc Multiplexed analysis of target nucleic acids
CA2929596C (en) * 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
SG10202004286UA (en) * 2014-05-08 2020-06-29 Fluidigm Corp Integrated single cell sequencing
US10829814B2 (en) * 2014-06-19 2020-11-10 Illumina, Inc. Methods and compositions for single cell genomics
EP4053292A1 (en) * 2014-06-26 2022-09-07 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
BR112017007912A2 (pt) * 2014-10-17 2018-01-23 Illumina Cambridge Limited transposon de preservação de contiguidade
EP4119677B1 (en) * 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CA3023566A1 (en) * 2016-05-16 2017-11-23 Nanostring Technologies, Inc. Methods for detecting target nucleic acids in a sample
US11299769B2 (en) * 2016-06-06 2022-04-12 Redvault Biosciences, Lp Target reporter constructs and uses thereof
JP7280181B2 (ja) * 2016-10-01 2023-05-23 バークレー ライツ，インコーポレイテッド Ｄｎａバーコード組成物及びマイクロ流体デバイスによるインサイチュ同定法
EP3601605B1 (en) * 2017-03-24 2023-11-22 National University of Singapore Methods for multiplex detection of molecules
WO2018186947A1 (en) * 2017-04-06 2018-10-11 MyOmicsDx, Inc Method and kit for targeted enrichment of nucleic acids

Also Published As

Publication number Publication date
JP2022503873A (ja) 2022-01-12
US20230313275A1 (en) 2023-10-05
BR112021006190A2 (pt) 2021-06-29
US11667957B2 (en) 2023-06-06
US20200131570A1 (en) 2020-04-30
KR20210084441A (ko) 2021-07-07
CN112840023A (zh) 2021-05-25
CN112840023B (zh) 2024-03-01
CA3113270A1 (en) 2020-04-30
WO2020086746A1 (en) 2020-04-30
IL281551A (en) 2021-05-31
SG11202102722SA (en) 2021-04-29
EP3870704A4 (en) 2023-01-11
AU2019364418A1 (en) 2021-04-22
CN117778535A (zh) 2024-03-29
EP3870704A1 (en) 2021-09-01

Similar Documents

Publication Publication Date Title
MX2021003768A (es) Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra.
HRP20201856T1 (hr) Antitijela koja se vežu za ljudski ligand i programiranje smrti (pd-l1)
CY1121291T1 (el) Ανοσοδοκιμασιες ενισχυσης σηματος
EA201070349A1 (ru) Определение вариаций количества копий, способы и системы
EA201370085A1 (ru) Новые системы и способы неразрушающего контроля самолетов
MX2009013388A (es) Analisis multiple de muestras de sangre.
WO2013003498A3 (en) Acoustic cytometry methods and protocols
NZ609363A (en) Methods for determining anti-drug antibody isotypes
EA202092900A3 (ru) Мутационный анализ днк в плазме для детектирования рака
RU2015123570A (ru) Цифровой анализ молекулярных анализируемых веществ с использованием одномолекулярного обнаружения
EP4083233A3 (en) Method for using gene expression to determine prognosis of prostate cancer
WO2010151842A3 (en) Methods and systems for phylogenetic analysis
WO2011109372A8 (en) Ultra-sensitive detection of molecules using dual detection methods
MX2021015885A (es) Métodos y composiciones para identificar ligandos en arreglos usando índices y códigos de barra.
HK1115636A1 (en) Use of cyfra 21-1 as a marker for colorectal cancer cyfra 21-1
JP2012073260A5 (es)
ATE370414T1 (de) Verwendung von nicotinamide n-methyltransferase zur diagnose von kolorektalem krebs
ATE426168T1 (de) Verwendung von asc als marker fur kolorektalkarzinome
CN105861652A (zh) 一种鹿源性物种成分的检测鉴定方法
FR2971336B1 (fr) Procede de detection et d'identification d'un analyte present dans un milieu gazeux
MX2016017048A (es) Inmunoensayo reactivo y colaborativo potenciado por enzimas (ceer) usando citometria de flujo.
RU2017107273A (ru) Антилимфоцитарные аутоантитела в качестве диагностических биомаркеров
DE602005014659D1 (de) Auf immunreaktion gegen boris basierendes verfahren für den nachweis von krebs
JP2012154899A5 (ja) ヒトil-17産生ヘルパーt細胞の検出方法
RU2007121028A (ru) Способ прогнозирования газодинамических процессов при подземной разработке месторождений, способ локации ловушек, применение индивидуальных индикаторов газа