MX2020003985A - Metodo de reconstruccion de imagenes, dispositivo y dispositivo de imagen microscopica. - Google Patents

Metodo de reconstruccion de imagenes, dispositivo y dispositivo de imagen microscopica.

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Publication number
MX2020003985A
MX2020003985A MX2020003985A MX2020003985A MX2020003985A MX 2020003985 A MX2020003985 A MX 2020003985A MX 2020003985 A MX2020003985 A MX 2020003985A MX 2020003985 A MX2020003985 A MX 2020003985A MX 2020003985 A MX2020003985 A MX 2020003985A
Authority
MX
Mexico
Prior art keywords
fiber
image
gray value
reconstructed image
image reconstruction
Prior art date
Application number
MX2020003985A
Other languages
English (en)
Inventor
Qiang Wang
Jinhua Shao
Jin Sun
Houli Duan
Original Assignee
Suzhou Microview Medical Tech Co Ltd
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Publication date
Application filed by Suzhou Microview Medical Tech Co Ltd filed Critical Suzhou Microview Medical Tech Co Ltd
Publication of MX2020003985A publication Critical patent/MX2020003985A/es

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T3/00—Geometric image transformations in the plane of the image
    • G06T3/40—Scaling of whole images or parts thereof, e.g. expanding or contracting
    • G06T3/4007—Scaling of whole images or parts thereof, e.g. expanding or contracting based on interpolation, e.g. bilinear interpolation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/02—Objectives
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T11/00—2D [Two Dimensional] image generation
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T3/00—Geometric image transformations in the plane of the image
    • G06T3/40—Scaling of whole images or parts thereof, e.g. expanding or contracting
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/90—Dynamic range modification of images or parts thereof
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N25/00—Circuitry of solid-state image sensors [SSIS]; Control thereof
    • H04N25/70—SSIS architectures; Circuits associated therewith
    • H04N25/71—Charge-coupled device [CCD] sensors; Charge-transfer registers specially adapted for CCD sensors
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N7/00—Television systems
    • H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/063—Illuminating optical parts
    • G01N2201/0635—Structured illumination, e.g. with grating
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/08—Optical fibres; light guides
    • G01N2201/0826—Fibre array at source, distributing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/08—Optical fibres; light guides
    • G01N2201/0833—Fibre array at detector, resolving
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Optics & Photonics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Signal Processing (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Image Processing (AREA)

Abstract

Se proporcionan un método de reconstrucción de imágenes, un dispositivo y un dispositivo de imágenes microscópicas. El método incluye calcular un valor de gris en cada centro de fibra en un haz de fibras (04) en una imagen reconstruida de acuerdo con un valor de gris en una posición central de cada fibra, determinado en una o más imágenes de muestra; realizar una interpolación espacial utilizando el valor de gris en el centro de la fibra para obtener valores de gris de otros puntos de pixeles en el haz de fibras (04) en la imagen reconstruida, para formar la imagen reconstruida. Este método de reconstrucción de imagen solo calcula el valor de gris del punto de pixel en la posición central de la fibra, y luego obtiene valores de gris de puntos de pixel de toda la imagen en función de la interpolación espacial, reduciendo así la cantidad de cálculo debido al cálculo del valor de gris de cada punto de pixel, acelerando en gran medida la velocidad de la reconstrucción de la imagen, y el método es útil para eliminar los residuos de la rejilla (022) y la rejilla cellular del haz de fibras (04) en la imagen reconstruida y mejorar la calidad de imagen de la imagen reconstruida.
MX2020003985A 2017-10-16 2018-09-29 Metodo de reconstruccion de imagenes, dispositivo y dispositivo de imagen microscopica. MX2020003985A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201710959434.8A CN107621463B (zh) 2017-10-16 2017-10-16 图像重建方法、装置及显微成像装置
PCT/CN2018/108865 WO2019076192A1 (zh) 2017-10-16 2018-09-29 图像重建方法、装置及显微成像装置

Publications (1)

Publication Number Publication Date
MX2020003985A true MX2020003985A (es) 2020-08-13

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MX2020003985A MX2020003985A (es) 2017-10-16 2018-09-29 Metodo de reconstruccion de imagenes, dispositivo y dispositivo de imagen microscopica.

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US (1) US11449964B2 (es)
EP (1) EP3699576A4 (es)
JP (1) JP7064796B2 (es)
KR (1) KR102358848B1 (es)
CN (1) CN107621463B (es)
AU (1) AU2018352821B2 (es)
BR (1) BR112020007609B1 (es)
CA (1) CA3079243C (es)
MX (1) MX2020003985A (es)
RU (1) RU2747129C1 (es)
WO (1) WO2019076192A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107622491B (zh) * 2017-10-16 2022-03-11 苏州微景医学科技有限公司 光纤束图像分析方法和装置
CN107621463B (zh) * 2017-10-16 2024-03-22 苏州微景医学科技有限公司 图像重建方法、装置及显微成像装置
CN111024659B (zh) * 2019-11-28 2020-12-11 浙江大学 一种基于并行探测的多图像重建显微成像方法和装置
CN111461975B (zh) * 2020-03-17 2021-04-23 中国科学院苏州生物医学工程技术研究所 光纤束超分辨成像方法、***、计算机设备和存储介质
CN113570520A (zh) * 2021-07-28 2021-10-29 苏州微景医学科技有限公司 光纤图像校正方法、装置和计算机可读存储介质

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436980A (en) * 1988-05-10 1995-07-25 E. I. Du Pont De Nemours And Company Method for determining quality of dispersion of glass fibers in a thermoplastic resin preform layer and preform layer characterized thereby
JPH04138127A (ja) * 1990-09-28 1992-05-12 Olympus Optical Co Ltd 内視鏡網目画像軽減装置
AUPQ685900A0 (en) * 2000-04-12 2000-05-11 Goyen Controls Co Pty Limited Method and apparatus for detecting particles in a gas flow
BR0316855A (pt) * 2002-12-05 2006-10-03 Ericsson Telefon Ab L M método e arranjo para calibrar temperatura de fusão em um dispositivo de emenda de fibra óptica
CN100417225C (zh) * 2005-10-27 2008-09-03 中国科学院上海技术物理研究所 基于光纤耦合的焦平面阵列图像时空变换的方法
DE102006011707B4 (de) * 2006-03-14 2010-11-18 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren und Vorrichtung zum Erzeugen einer strukturfreien fiberskopischen Aufnahme
CN100481937C (zh) * 2006-05-12 2009-04-22 北京理工大学 一种高动态、超分辨率图像重建装置
DE102007018048A1 (de) * 2007-04-13 2008-10-16 Michael Schwertner Verfahren und Anordnung zur optischen Abbildung mit Tiefendiskriminierung
DE102007026595A1 (de) * 2007-06-08 2008-12-18 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung und Verfahren zur Kompensation von Farbverschiebungen in faseroptischen Abbildungssystemen
CN101802675B (zh) * 2007-07-06 2014-09-17 新加坡国立大学 荧光焦点调制显微***和方法
TWI426296B (zh) * 2009-06-19 2014-02-11 Ind Tech Res Inst 利用光學偏極特性之三維顯微共焦量測系統與方法
CN101692971B (zh) * 2009-10-13 2011-04-06 西安电子科技大学 非接触式光学断层成像方法
JP5343065B2 (ja) * 2010-12-07 2013-11-13 富士フイルム株式会社 放射線撮影システム
KR101260051B1 (ko) * 2012-01-30 2013-05-06 한국화학연구원 살아있는 세포에 대한 명시야 이미징 및 형광 이미징의 동시 수행이 가능한 세포 이미징 장치 및 방법
JP5951293B2 (ja) * 2012-03-14 2016-07-13 日立Ｇｅニュークリア・エナジー株式会社 画像処理方法および装置
EP2870498A4 (en) * 2012-07-05 2016-03-02 Martin Russell Harris STRUCTURED LIGHTING MICROSCOPE AND METHOD
US10096098B2 (en) * 2013-12-30 2018-10-09 Carestream Health, Inc. Phase retrieval from differential phase contrast imaging
CN103592722B (zh) * 2013-08-22 2015-08-05 浙江大学 一种熊猫型保偏光纤侧视对轴装置及方法
CN103438802B (zh) * 2013-09-17 2016-04-20 上海理工大学 光纤涂覆层几何参数测量方法
US10419767B2 (en) * 2014-02-21 2019-09-17 Koninklijke Philips N.V. Encoding video with the luminances of the pixel colors converted into lumas with a predetermined code allocation and decoding the video
JP2016109579A (ja) * 2014-12-08 2016-06-20 ソニー株式会社 情報処理装置、画像取得システム、情報処理方法、画像情報取得方法及びプログラム
CN104794475B (zh) * 2015-04-03 2018-04-27 燕山大学 一种光子晶体光纤的端面结构特征提取方法
CN106447717B (zh) * 2016-09-30 2019-05-03 中国科学院自动化研究所 一种基于多角度的选择性光片照明显微成像的重建方法
CN106981090B (zh) * 2017-02-16 2020-04-28 南京邮电大学 一种管内步进单向光束扫描断层图像的三维重建方法
CN107621463B (zh) 2017-10-16 2024-03-22 苏州微景医学科技有限公司 图像重建方法、装置及显微成像装置

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JP7064796B2 (ja) 2022-05-11
CN107621463A (zh) 2018-01-23
KR20200070313A (ko) 2020-06-17
EP3699576A4 (en) 2020-11-25
AU2018352821B2 (en) 2021-09-16
BR112020007609A2 (pt) 2020-11-10
EP3699576A1 (en) 2020-08-26
CA3079243C (en) 2023-05-16
JP2020537162A (ja) 2020-12-17
BR112020007609B1 (pt) 2023-11-28
US20200242732A1 (en) 2020-07-30
RU2747129C1 (ru) 2021-04-28
CN107621463B (zh) 2024-03-22
AU2018352821A1 (en) 2020-05-14
CA3079243A1 (en) 2019-04-25
US11449964B2 (en) 2022-09-20
KR102358848B1 (ko) 2022-02-08
WO2019076192A1 (zh) 2019-04-25

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