MX2020003362A - Cepas modificadas para la produccion de seda recombinante. - Google Patents

Cepas modificadas para la produccion de seda recombinante.

Info

Publication number
MX2020003362A
MX2020003362A MX2020003362A MX2020003362A MX2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A
Authority
MX
Mexico
Prior art keywords
modified strains
production
recombinant silk
strains
modified
Prior art date
Application number
MX2020003362A
Other languages
English (en)
Inventor
Matthew Scott Gamboa
Joshua Tyler Kittleson
Original Assignee
Bolt Threads Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bolt Threads Inc filed Critical Bolt Threads Inc
Publication of MX2020003362A publication Critical patent/MX2020003362A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23041—Yapsin 1 (3.4.23.41)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Insects & Arthropods (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

En la presente se describen cepas modificadas para reducir la degradación de productos que se expresan de forma recombinante secretados por un organismo hospedador y métodos para el uso de las cepas modificadas.
MX2020003362A 2017-10-03 2017-10-03 Cepas modificadas para la produccion de seda recombinante. MX2020003362A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2017/054997 WO2019070246A1 (en) 2017-10-03 2017-10-03 MODIFIED STRAINS FOR RECOMBINANT SILK PRODUCTION

Publications (1)

Publication Number Publication Date
MX2020003362A true MX2020003362A (es) 2020-07-29

Family

ID=65994932

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020003362A MX2020003362A (es) 2017-10-03 2017-10-03 Cepas modificadas para la produccion de seda recombinante.

Country Status (7)

Country Link
EP (1) EP3692054A4 (es)
JP (1) JP7246102B2 (es)
KR (1) KR102558303B1 (es)
CN (1) CN111315763A (es)
AU (2) AU2017434920B2 (es)
MX (1) MX2020003362A (es)
WO (1) WO2019070246A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019222633A1 (en) 2018-05-17 2019-11-21 Bolt Threads, Inc. Sec modified strains for improved secretion of recombinant proteins
WO2023215221A2 (en) * 2022-05-02 2023-11-09 North Carolina State University Engineered microorganisms with enhanced protein expression and secretion

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003091431A1 (fr) * 2002-04-26 2003-11-06 Kirin Beer Kabushiki Kaisha Methylotrophe produisant une chaine de sucre de type mammifere
KR100496758B1 (ko) * 2002-06-29 2005-06-22 한국생명공학연구원 한세눌라 폴리모르파 앱신 유전자 결손 변이주 및 이를이용한 재조합 단백질 생산 방법
CA2499467A1 (en) * 2002-09-20 2004-04-01 Cym1P A/S Methods for increasing the production of a recombinant polypeptide from a host cell
ES2539378T3 (es) 2009-02-26 2015-06-30 Glaxosmithkline Llc Células huésped y procedimientos de uso
US8440456B2 (en) * 2009-05-22 2013-05-14 Vib, Vzw Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins
KR20140114818A (ko) * 2011-12-30 2014-09-29 옥시레인 유케이 리미티드 재조합 단백질들의 분해를 감소시키기 위한 방법들 및 물질들
CN102676563B (zh) * 2012-05-03 2013-09-18 浙江大学 一种制备人血清白蛋白-人甲状旁腺激素的方法
ES2887366T3 (es) * 2013-09-17 2021-12-22 Bolt Threads Inc Métodos y composiciones para sintetizar fibras de seda mejoradas
CN106701813A (zh) * 2016-12-29 2017-05-24 陕西慧康生物科技有限责任公司 表达载体及其构建方法与应用

Also Published As

Publication number Publication date
AU2022202534A1 (en) 2022-05-12
JP2021503275A (ja) 2021-02-12
CN111315763A (zh) 2020-06-19
KR20200058482A (ko) 2020-05-27
EP3692054A1 (en) 2020-08-12
AU2017434920A1 (en) 2020-05-07
EP3692054A4 (en) 2021-06-09
KR102558303B1 (ko) 2023-07-21
WO2019070246A1 (en) 2019-04-11
JP7246102B2 (ja) 2023-03-27
AU2017434920B2 (en) 2022-02-10

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