MX2019000743A - Variantes de serinproteasa y polinucleotidos que las codifican. - Google Patents
Variantes de serinproteasa y polinucleotidos que las codifican.Info
- Publication number
- MX2019000743A MX2019000743A MX2019000743A MX2019000743A MX2019000743A MX 2019000743 A MX2019000743 A MX 2019000743A MX 2019000743 A MX2019000743 A MX 2019000743A MX 2019000743 A MX2019000743 A MX 2019000743A MX 2019000743 A MX2019000743 A MX 2019000743A
- Authority
- MX
- Mexico
- Prior art keywords
- variants
- serine protease
- polynucleotides encoding
- increased
- protease variants
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
Abstract
La presente invención se refiere a variantes de serinproteasa que tienen una mejora de las propiedades en comparación con la proteasa progenitora, en particular variantes de una serinproteasa obtenida a partir de una cepa de Meripilus giganteus que pertenece a la familia S53. Las variantes de acuerdo con la invención presentan en particular un incremento de la estabilidad, por ejemplo, incremento de la termoestabilidad, incremento de la actividad específica y/o incremento de los niveles de expresión, en comparación con la proteasa progenitora. La presente invención también se refiere a polinucleótidos que codifican las variantes; a constructos de ácido nucleico, vectores y células hospedadoras que comprenden los polinucleótidos; y a métodos para usar las variantes.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP16180498 | 2016-07-21 | ||
EP16195087 | 2016-10-21 | ||
PCT/EP2017/067881 WO2018015303A1 (en) | 2016-07-21 | 2017-07-14 | Serine protease variants and polynucleotides encoding same |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2019000743A true MX2019000743A (es) | 2019-05-20 |
Family
ID=59501395
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2019000743A MX2019000743A (es) | 2016-07-21 | 2017-07-14 | Variantes de serinproteasa y polinucleotidos que las codifican. |
Country Status (8)
Country | Link |
---|---|
US (1) | US11891645B2 (es) |
EP (1) | EP3487995B1 (es) |
CN (1) | CN109661465A (es) |
CA (1) | CA3029479A1 (es) |
DK (1) | DK3487995T3 (es) |
ES (1) | ES2884155T3 (es) |
MX (1) | MX2019000743A (es) |
WO (1) | WO2018015303A1 (es) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA3028875A1 (en) * | 2016-07-21 | 2018-01-25 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
EP3487995B1 (en) | 2016-07-21 | 2021-05-26 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
KR102316445B1 (ko) * | 2019-11-29 | 2021-10-26 | 씨제이제일제당 주식회사 | 신규 세린 프로테아제 변이체 |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
WO2024137704A2 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast |
WO2024137250A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
WO2024137248A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization |
WO2024137252A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Process for reducing syrup viscosity in the backend of a process for producing a fermentation product |
WO2024137246A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same |
Family Cites Families (36)
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US3912590A (en) | 1973-01-03 | 1975-10-14 | Novo Industri As | Procedure for liquefying starch |
US4316956A (en) | 1980-02-06 | 1982-02-23 | Novo Industri A/S | Fermentation process |
US4335208A (en) | 1980-03-11 | 1982-06-15 | Novo Industri A/S | Saccharification of starch hydrolysates |
JPS57174089A (en) | 1981-04-20 | 1982-10-26 | Novo Industri As | Chain dividing enzyme product |
DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
EP0252730B1 (en) | 1986-07-09 | 1992-09-16 | Novo Nordisk A/S | Alpha-amylase mixtures for starch liquefaction |
US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
JP3016587B2 (ja) | 1989-12-04 | 2000-03-06 | ザ・リサーチ・ファンデーション・オブ・ステート・ユニバーシティ・オブ・ニューヨーク | 非ステロイド抗炎症剤及びテトラサイクリンの配合 |
IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
US5231017A (en) | 1991-05-17 | 1993-07-27 | Solvay Enzymes, Inc. | Process for producing ethanol |
DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
DK0765394T3 (da) | 1994-06-03 | 2001-12-10 | Novozymes As | Oprensede Myceliopthora-laccaser og nukleinsyrer der koder for disse |
AU2705895A (en) | 1994-06-30 | 1996-01-25 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein |
JP4358431B2 (ja) | 1997-10-13 | 2009-11-04 | ノボザイムス アクティーゼルスカブ | α−アミラーゼ変異体 |
CA2344619C (en) | 1998-10-26 | 2012-01-03 | Novozymes A/S | Constructing and screening a dna library of interest in filamentous fungal cells |
CN100510096C (zh) | 1999-03-22 | 2009-07-08 | 诺沃奇梅兹有限公司 | 用于在真菌细胞中表达基因的启动子 |
US7314712B2 (en) | 2001-07-27 | 2008-01-01 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Systems for in vivo site-directed mutagenesis using oligonucleotides |
PT1468093E (pt) | 2002-01-23 | 2009-04-15 | Royal Nedalco B V | Fermentação de açúcares pentose |
JP2005523689A (ja) | 2002-02-08 | 2005-08-11 | ジェネンコー・インターナショナル・インク | 炭素基質からエタノールを生成する方法 |
WO2004085627A1 (en) | 2003-03-26 | 2004-10-07 | Forskarpatent I Syd Ab | New saccharomyces cerevisiae strains utilizing xylose |
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ES2551515T3 (es) | 2008-03-07 | 2015-11-19 | Dsm Ip Assets B.V. | Una célula fermentadora de azúcar pentosa |
WO2010022045A1 (en) | 2008-08-20 | 2010-02-25 | Novozymes A/S | Processes for producing fermentation products |
WO2010074577A1 (en) | 2008-12-24 | 2010-07-01 | Royal Nedalco B.V. | Xylose isomerase genes and their use in fermentation of pentose sugars |
ES2534098T3 (es) | 2009-11-30 | 2015-04-17 | Novozymes A/S | Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos |
MX2012006176A (es) | 2009-12-01 | 2012-06-25 | Novozymes North America Inc | Polipeptidos que tienen actividad de glucoamilasa y polinucleotidos que codifican los mismos. |
CA2783820C (en) | 2009-12-11 | 2018-10-23 | Novozymes A/S | Protease variants |
CN103298932B (zh) | 2010-11-08 | 2016-08-10 | 诺维信公司 | 具有葡糖淀粉酶活性的多肽和编码该多肽的多核苷酸 |
CA2840962C (en) | 2011-07-06 | 2021-04-13 | Novozymes A/S | Alpha amylase variants and polynucleotides encoding same |
WO2013119302A2 (en) | 2011-11-21 | 2013-08-15 | Novozymes, Inc. | Gh61 polypeptide variants and polynucleotides encoding same |
WO2014037438A1 (en) | 2012-09-05 | 2014-03-13 | Novozymes A/S | Polypeptides having protease activity |
WO2015197871A1 (en) * | 2014-06-27 | 2015-12-30 | Dsm Ip Assets B.V. | A method for improving the nutritional value of animal feed |
EP3353292A4 (en) | 2015-09-25 | 2019-04-03 | Novozymes A/S | USE OF SERINE PROTEASE TO ENHANCE ETHANOL YIELD |
CA3028875A1 (en) | 2016-07-21 | 2018-01-25 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
EP3487995B1 (en) | 2016-07-21 | 2021-05-26 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
-
2017
- 2017-07-14 EP EP17746004.5A patent/EP3487995B1/en active Active
- 2017-07-14 MX MX2019000743A patent/MX2019000743A/es unknown
- 2017-07-14 ES ES17746004T patent/ES2884155T3/es active Active
- 2017-07-14 CA CA3029479A patent/CA3029479A1/en active Pending
- 2017-07-14 US US16/318,509 patent/US11891645B2/en active Active
- 2017-07-14 WO PCT/EP2017/067881 patent/WO2018015303A1/en unknown
- 2017-07-14 DK DK17746004.5T patent/DK3487995T3/da active
- 2017-07-14 CN CN201780043881.7A patent/CN109661465A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
EP3487995B1 (en) | 2021-05-26 |
ES2884155T3 (es) | 2021-12-10 |
CA3029479A1 (en) | 2018-01-25 |
DK3487995T3 (da) | 2021-07-26 |
WO2018015303A1 (en) | 2018-01-25 |
EP3487995A1 (en) | 2019-05-29 |
US11891645B2 (en) | 2024-02-06 |
CN109661465A (zh) | 2019-04-19 |
US20230193327A1 (en) | 2023-06-22 |
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