MX2019000743A - Variantes de serinproteasa y polinucleotidos que las codifican. - Google Patents

Variantes de serinproteasa y polinucleotidos que las codifican.

Info

Publication number
MX2019000743A
MX2019000743A MX2019000743A MX2019000743A MX2019000743A MX 2019000743 A MX2019000743 A MX 2019000743A MX 2019000743 A MX2019000743 A MX 2019000743A MX 2019000743 A MX2019000743 A MX 2019000743A MX 2019000743 A MX2019000743 A MX 2019000743A
Authority
MX
Mexico
Prior art keywords
variants
serine protease
polynucleotides encoding
increased
protease variants
Prior art date
Application number
MX2019000743A
Other languages
English (en)
Inventor
M K Klitgaard Dorte
Alexander Pache Roland
Gudmand Martin
G Sandström Andreas
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of MX2019000743A publication Critical patent/MX2019000743A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

La presente invención se refiere a variantes de serinproteasa que tienen una mejora de las propiedades en comparación con la proteasa progenitora, en particular variantes de una serinproteasa obtenida a partir de una cepa de Meripilus giganteus que pertenece a la familia S53. Las variantes de acuerdo con la invención presentan en particular un incremento de la estabilidad, por ejemplo, incremento de la termoestabilidad, incremento de la actividad específica y/o incremento de los niveles de expresión, en comparación con la proteasa progenitora. La presente invención también se refiere a polinucleótidos que codifican las variantes; a constructos de ácido nucleico, vectores y células hospedadoras que comprenden los polinucleótidos; y a métodos para usar las variantes.
MX2019000743A 2016-07-21 2017-07-14 Variantes de serinproteasa y polinucleotidos que las codifican. MX2019000743A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16180498 2016-07-21
EP16195087 2016-10-21
PCT/EP2017/067881 WO2018015303A1 (en) 2016-07-21 2017-07-14 Serine protease variants and polynucleotides encoding same

Publications (1)

Publication Number Publication Date
MX2019000743A true MX2019000743A (es) 2019-05-20

Family

ID=59501395

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019000743A MX2019000743A (es) 2016-07-21 2017-07-14 Variantes de serinproteasa y polinucleotidos que las codifican.

Country Status (8)

Country Link
US (1) US11891645B2 (es)
EP (1) EP3487995B1 (es)
CN (1) CN109661465A (es)
CA (1) CA3029479A1 (es)
DK (1) DK3487995T3 (es)
ES (1) ES2884155T3 (es)
MX (1) MX2019000743A (es)
WO (1) WO2018015303A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3028875A1 (en) * 2016-07-21 2018-01-25 Novozymes A/S Serine protease variants and polynucleotides encoding same
EP3487995B1 (en) 2016-07-21 2021-05-26 Novozymes A/S Serine protease variants and polynucleotides encoding same
KR102316445B1 (ko) * 2019-11-29 2021-10-26 씨제이제일제당 주식회사 신규 세린 프로테아제 변이체
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2024137704A2 (en) 2022-12-19 2024-06-27 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
WO2024137250A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
WO2024137248A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
WO2024137246A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3912590A (en) 1973-01-03 1975-10-14 Novo Industri As Procedure for liquefying starch
US4316956A (en) 1980-02-06 1982-02-23 Novo Industri A/S Fermentation process
US4335208A (en) 1980-03-11 1982-06-15 Novo Industri A/S Saccharification of starch hydrolysates
JPS57174089A (en) 1981-04-20 1982-10-26 Novo Industri As Chain dividing enzyme product
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
EP0252730B1 (en) 1986-07-09 1992-09-16 Novo Nordisk A/S Alpha-amylase mixtures for starch liquefaction
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
JP3016587B2 (ja) 1989-12-04 2000-03-06 ザ・リサーチ・ファンデーション・オブ・ステート・ユニバーシティ・オブ・ニューヨーク 非ステロイド抗炎症剤及びテトラサイクリンの配合
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
US5231017A (en) 1991-05-17 1993-07-27 Solvay Enzymes, Inc. Process for producing ethanol
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DK0765394T3 (da) 1994-06-03 2001-12-10 Novozymes As Oprensede Myceliopthora-laccaser og nukleinsyrer der koder for disse
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
JP4358431B2 (ja) 1997-10-13 2009-11-04 ノボザイムス アクティーゼルスカブ α−アミラーゼ変異体
CA2344619C (en) 1998-10-26 2012-01-03 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
CN100510096C (zh) 1999-03-22 2009-07-08 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
US7314712B2 (en) 2001-07-27 2008-01-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
PT1468093E (pt) 2002-01-23 2009-04-15 Royal Nedalco B V Fermentação de açúcares pentose
JP2005523689A (ja) 2002-02-08 2005-08-11 ジェネンコー・インターナショナル・インク 炭素基質からエタノールを生成する方法
WO2004085627A1 (en) 2003-03-26 2004-10-07 Forskarpatent I Syd Ab New saccharomyces cerevisiae strains utilizing xylose
US7968318B2 (en) 2006-06-06 2011-06-28 Genencor International, Inc. Process for conversion of granular starch to ethanol
ES2551515T3 (es) 2008-03-07 2015-11-19 Dsm Ip Assets B.V. Una célula fermentadora de azúcar pentosa
WO2010022045A1 (en) 2008-08-20 2010-02-25 Novozymes A/S Processes for producing fermentation products
WO2010074577A1 (en) 2008-12-24 2010-07-01 Royal Nedalco B.V. Xylose isomerase genes and their use in fermentation of pentose sugars
ES2534098T3 (es) 2009-11-30 2015-04-17 Novozymes A/S Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos
MX2012006176A (es) 2009-12-01 2012-06-25 Novozymes North America Inc Polipeptidos que tienen actividad de glucoamilasa y polinucleotidos que codifican los mismos.
CA2783820C (en) 2009-12-11 2018-10-23 Novozymes A/S Protease variants
CN103298932B (zh) 2010-11-08 2016-08-10 诺维信公司 具有葡糖淀粉酶活性的多肽和编码该多肽的多核苷酸
CA2840962C (en) 2011-07-06 2021-04-13 Novozymes A/S Alpha amylase variants and polynucleotides encoding same
WO2013119302A2 (en) 2011-11-21 2013-08-15 Novozymes, Inc. Gh61 polypeptide variants and polynucleotides encoding same
WO2014037438A1 (en) 2012-09-05 2014-03-13 Novozymes A/S Polypeptides having protease activity
WO2015197871A1 (en) * 2014-06-27 2015-12-30 Dsm Ip Assets B.V. A method for improving the nutritional value of animal feed
EP3353292A4 (en) 2015-09-25 2019-04-03 Novozymes A/S USE OF SERINE PROTEASE TO ENHANCE ETHANOL YIELD
CA3028875A1 (en) 2016-07-21 2018-01-25 Novozymes A/S Serine protease variants and polynucleotides encoding same
EP3487995B1 (en) 2016-07-21 2021-05-26 Novozymes A/S Serine protease variants and polynucleotides encoding same

Also Published As

Publication number Publication date
EP3487995B1 (en) 2021-05-26
ES2884155T3 (es) 2021-12-10
CA3029479A1 (en) 2018-01-25
DK3487995T3 (da) 2021-07-26
WO2018015303A1 (en) 2018-01-25
EP3487995A1 (en) 2019-05-29
US11891645B2 (en) 2024-02-06
CN109661465A (zh) 2019-04-19
US20230193327A1 (en) 2023-06-22

Similar Documents

Publication Publication Date Title
MX2019000743A (es) Variantes de serinproteasa y polinucleotidos que las codifican.
MX2017014125A (es) Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.
MX2019000805A (es) Variantes de serinproteasa y polinucleotidos que las codifican.
EP3741849A3 (en) Protease variants and polynucleotides encoding same
MX2023001119A (es) Variantes de enzimas y polinucleotidos que las codifican.
WO2016066756A3 (en) Protease variants and polynucleotides encoding same
WO2016066757A3 (en) Protease variants and polynucleotides encoding same
EP3741848A3 (en) Protease variants and polynucleotides encoding same
MX2019000140A (es) Variantes de xilanasa y polinucleotidos que las codifican.
MX2018008051A (es) Variantes de beta-glucanasa y polinucleotidos que las codifican.
ZA201905673B (en) Alpha-amylase variants
MX2019008780A (es) Composiciones detergentes.
EA202090540A1 (ru) Ген, придающий устойчивость к патогенному грибу
WO2012101206A3 (en) Novel glycoside hydrolases from thermophilic fungi
MX351916B (es) Polipeptidos que tienen actividad de lisozima y polinucleotdos que los codifican.
WO2012146171A9 (en) Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2015181119A3 (en) Lipase variants and polynucleotides encoding same
EP3964575A3 (en) Alpha-amylase variants and polynucleotides encoding same
MX363261B (es) Polipéptidos que tienen actividad de la fosfolipasa a y polinucleótidos que los codifican.
MX2017007105A (es) Variantes de lipasa y polinucleotidos que las codifican.
MX349517B (es) Polipeptidos que tienen actividad de proteasa y polinucleotidos que codifican los mismos.
MX2019014889A (es) Polipeptidos que tienen actividad trehalasa y polinucleotidos que los codifican.
MX2021013879A (es) Variantes de lipasa y polinucleotidos que las codifican.
EA201990034A1 (ru) Ферментативное гликозилирование стевиолгликозидов и других соединений с глюкозо-1-фосфатом
MX350391B (es) Polipeptidos que poseen actividad proteasa y polinucleotidos que los codifican.