MX2014015277A - Elementos de adn baculoviral para la expresion de proteinas recombinantes en una celula huesped. - Google Patents

Elementos de adn baculoviral para la expresion de proteinas recombinantes en una celula huesped.

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Publication number
MX2014015277A
MX2014015277A MX2014015277A MX2014015277A MX2014015277A MX 2014015277 A MX2014015277 A MX 2014015277A MX 2014015277 A MX2014015277 A MX 2014015277A MX 2014015277 A MX2014015277 A MX 2014015277A MX 2014015277 A MX2014015277 A MX 2014015277A
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Mexico
Prior art keywords
host cell
dna elements
expression
recombinant
elements
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MX2014015277A
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English (en)
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MX353498B (es
Inventor
Silvia Gomez Sebastian
Javier López Vidal
José Angel Martinez Escribano
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Alternative Gene Expression Sl
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Application filed by Alternative Gene Expression Sl filed Critical Alternative Gene Expression Sl
Publication of MX2014015277A publication Critical patent/MX2014015277A/es
Publication of MX353498B publication Critical patent/MX353498B/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/866—Baculoviral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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    • A01K2267/02—Animal zootechnically ameliorated
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    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Se proporcionan reactivos y métodos que permiten una expresión mejorada de una proteína recombinante. Más específicamente, la introducción de elementos de ADN recombinante en una célula huésped permite la expresión incrementada de una proteína recombinante, una mejora del correcto plegamiento de dicha proteína y un incremento en la viabilidad y proliferación de célula de la célula huésped. Estos elementos de ADN recombinante pueden ser introducidos en células huésped, por ejemplo, a través de un baculovirus recombinante, el cual tiene incorporados dichos elementos. Los elementos de ADN recombinante incluyen ácidos nucleicos que codifican reguladores de transcripción, tales como lE-0 e lE-1, elementos mejoradores de transcripción, tales como la región homóloga (hr) y promotores.
MX2014015277A 2011-06-10 2012-06-12 Elementos de adn baculoviral para la expresion de proteinas recombinantes en una celula huesped. MX353498B (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP11169634 2011-06-10
EP2011068381 2011-10-20
EP2011072406 2011-12-12
PCT/EP2012/061081 WO2012168492A2 (en) 2011-06-10 2012-06-12 Recombinant dna elements for the expression of recombinant proteins in a host cell

Publications (2)

Publication Number Publication Date
MX2014015277A true MX2014015277A (es) 2015-12-15
MX353498B MX353498B (es) 2018-01-16

Family

ID=47296540

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014015277A MX353498B (es) 2011-06-10 2012-06-12 Elementos de adn baculoviral para la expresion de proteinas recombinantes en una celula huesped.

Country Status (9)

Country Link
EP (2) EP2858490B1 (es)
BR (1) BR112014031039A2 (es)
CA (2) CA2876641C (es)
DK (2) DK2858489T3 (es)
IN (1) IN2014MN02500A (es)
MX (1) MX353498B (es)
PL (2) PL2858489T3 (es)
PT (2) PT2858489T (es)
WO (6) WO2012168789A2 (es)

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* Cited by examiner, † Cited by third party
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ES2611302T3 (es) * 2012-06-12 2017-05-08 Alternative Gene Expression, S.L. Elementos de ADN recombinante para la expresión de proteínas recombinantes en insectos
CN105392362B (zh) 2012-06-12 2018-11-09 替代基因表达公司 用于在宿主昆虫中表达重组蛋白的杆状病毒dna元件
CN104735977B (zh) 2012-06-12 2016-09-21 替代基因表达公司 用于在宿主细胞中表达重组蛋白的杆状病毒dna元件
CN103749393A (zh) * 2013-12-20 2014-04-30 铜陵桂生生态养殖有限公司 一种降低蝎子死亡率的养殖方法
ES2554561B1 (es) * 2014-06-16 2016-09-30 Universitat De València Nuevos promotores derivados de baculovirus con elevada actividad en sistemas de expresión basados en baculovirus
RS59580B1 (sr) 2015-09-17 2019-12-31 Alternative Gene Expressions S L Ekspresija rekombinantnih proteina kod lutaka trichoplusia ni
CN105087606A (zh) * 2015-09-23 2015-11-25 天津瑞普生物技术股份有限公司 猪圆环病毒2型cap抗原制备方法
CN105238816B (zh) * 2015-10-26 2018-08-24 广州汉腾生物科技有限公司 一种表达载体
WO2018176075A1 (en) * 2017-03-27 2018-10-04 The University Of Queensland Chimeric insect-specific flaviviruses
EP3583948B1 (en) 2018-06-20 2022-04-06 FATRO S.p.A. Vaccines for the prevention of rabbit haemorrhagic disease
CN110964749A (zh) * 2018-09-29 2020-04-07 普莱柯生物工程股份有限公司 一种在杆状病毒表达***中高效表达外源蛋白的方法及其应用

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CA2179394A1 (en) * 1993-12-23 1995-06-29 Kostas Iatrou Methods of expressing proteins in insect cells and methods of killing insects
US5965393A (en) * 1997-07-01 1999-10-12 National Institute Of Immunology Method for enhancing foreign gene expression in baculovirus expression vector system
RU2158505C2 (ru) * 1998-06-09 2000-11-10 Совхоз "Тепличный" Способ приготовления углеводного корма для насекомых-опылителей
ES2232308B1 (es) 2003-11-12 2006-10-16 Laboratorios Calier, S.A. Dispositivo para la cria de larvas.
JP4271122B2 (ja) * 2004-10-15 2009-06-03 財団法人 ひろしま産業振興機構 カイコでの組換えタンパク質製造のためのポリヌクレオチド
US8148129B2 (en) * 2006-06-30 2012-04-03 The Regents Of The University Of California Generation of potent dominant negative transcriptional inhibitors
US8105827B2 (en) * 2007-09-06 2012-01-31 Academia Sinica Protein expression systems
BRPI0823074A2 (pt) * 2008-09-02 2012-04-10 Alternative Gene Expression S L Algenex método para desenvolver sequências regulatórias polinucleotìdicas, sequências regulatórias polinucleotìdicas, uso das sequências regulatórias polinucleotìdicas, vetor de expressão, células transformadas ou transfectadas, larvas de inseto transfectadas, método para produzir proteìnas recombinantes, uso do vetor de expressão e uso das larvas de inseto

Also Published As

Publication number Publication date
WO2012168492A2 (en) 2012-12-13
WO2012169939A2 (en) 2012-12-13
MX353498B (es) 2018-01-16
WO2012168789A2 (en) 2012-12-13
WO2012168788A2 (en) 2012-12-13
WO2012169939A3 (en) 2013-05-10
WO2012168788A3 (en) 2013-05-10
BR112014031039A2 (pt) 2018-05-08
WO2012169940A9 (en) 2013-02-14
DK2858489T3 (da) 2017-01-02
WO2012168493A3 (en) 2013-05-23
PL2858490T3 (pl) 2017-08-31
EP2858490B1 (en) 2016-10-26
CA2876641A1 (en) 2012-12-13
PT2858489T (pt) 2016-12-14
IN2014MN02500A (es) 2015-07-17
CA2876343A1 (en) 2012-12-13
EP2858490A2 (en) 2015-04-15
PT2858490T (pt) 2016-11-28
CA2876641C (en) 2018-09-25
WO2012168492A3 (en) 2013-04-11
WO2012168493A8 (en) 2013-04-04
WO2012168493A2 (en) 2012-12-13
DK2858490T5 (en) 2017-01-23
WO2012169939A9 (en) 2013-02-14
WO2012168789A3 (en) 2013-05-10
PL2858489T3 (pl) 2017-07-31
EP2858489B1 (en) 2016-11-09
DK2858490T3 (en) 2016-12-12
WO2012169940A2 (en) 2012-12-13
WO2012169940A3 (en) 2013-04-04
EP2858489A2 (en) 2015-04-15

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