MX2014006118A - Deteccion de analitos. - Google Patents

Deteccion de analitos.

Info

Publication number
MX2014006118A
MX2014006118A MX2014006118A MX2014006118A MX2014006118A MX 2014006118 A MX2014006118 A MX 2014006118A MX 2014006118 A MX2014006118 A MX 2014006118A MX 2014006118 A MX2014006118 A MX 2014006118A MX 2014006118 A MX2014006118 A MX 2014006118A
Authority
MX
Mexico
Prior art keywords
sample
analyte
eis
measurement
detecting analytes
Prior art date
Application number
MX2014006118A
Other languages
English (en)
Inventor
Holger Schulze
Damion Corrigan
Till Bachmann
Original Assignee
Iti Scotland Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Iti Scotland Ltd filed Critical Iti Scotland Ltd
Publication of MX2014006118A publication Critical patent/MX2014006118A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Se proporciona un método para detectar analito en una muestra, este método comprende: (a) poner en contacto la muestra con una sonda péptido ácido nucleico (PNA); (b) realizar una medición de espectrometría de impedancia electroquímica (EIS) en la muestra; (c) determinar la presencia, ausencia, cantidad y/o identidad del analito de la medición EIS; en donde el analito comprende ácido nucleico; y en donde la cantidad de analito en la muestra cuando la muestra se toma es sustancialmente la misma que la cantidad del analito en la muestra cuando la muestra se somete a la medición EIS.
MX2014006118A 2011-11-22 2012-11-21 Deteccion de analitos. MX2014006118A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1120118.3A GB201120118D0 (en) 2011-11-22 2011-11-22 Detecting analytes
GB1212662.9A GB2496932A (en) 2011-11-22 2012-07-17 Nucleic acid detection using electrochemical impedance spectroscopy
PCT/EP2012/073240 WO2013076143A1 (en) 2011-11-22 2012-11-21 Detecting analytes

Publications (1)

Publication Number Publication Date
MX2014006118A true MX2014006118A (es) 2015-04-16

Family

ID=45475523

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014006118A MX2014006118A (es) 2011-11-22 2012-11-21 Deteccion de analitos.

Country Status (13)

Country Link
US (1) US9777337B2 (es)
EP (1) EP2776583A1 (es)
JP (1) JP2015503095A (es)
KR (1) KR20140096104A (es)
CN (1) CN104145026A (es)
AU (1) AU2012342522A1 (es)
BR (1) BR112014012045A2 (es)
GB (2) GB201120118D0 (es)
IN (1) IN2014CN03508A (es)
MX (1) MX2014006118A (es)
RU (1) RU2014125212A (es)
WO (1) WO2013076143A1 (es)
ZA (1) ZA201404463B (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201415864D0 (en) 2014-09-08 2014-10-22 Univ Edinburgh Methods of detecting of multidrug resistant bacteria
EP3045895A1 (en) * 2015-01-13 2016-07-20 ARKRAY, Inc. Plasma spectrochemical analysis method and plasma spectrochemical analyzer
CN107430016B (zh) * 2015-02-06 2021-08-03 吉纳普赛斯股份有限公司 用于检测和分析生物物质的***和方法
CN107209134B (zh) * 2015-02-20 2020-05-26 索尼公司 电特性测量设备、电特性测量***、电特性测量方法以及用于使计算机实现所述方法的电特性测量程序
CN104914151B (zh) * 2015-05-08 2017-10-13 首都师范大学 一种用于氨苄西林和磺胺地索辛电化学传感器自组装钝化层的形成方法及其电化学传感器
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
US10012645B2 (en) * 2016-08-24 2018-07-03 The Florida International University Board Of Trustees Rapid zika virus detection using nano-enabled electrochemical sensing system
US11435338B2 (en) * 2016-10-24 2022-09-06 Ontera Inc. Fractional abundance of polynucleotide sequences in a sample
JP6803629B2 (ja) * 2017-07-31 2021-01-06 国立大学法人大阪大学 溶液中に存在する対象物質の検出方法および検出するためのシステム
CN108152250B (zh) * 2017-10-27 2020-02-18 南京邮电大学 生物识别探针的构建方法及其逻辑运算方法
KR102294374B1 (ko) * 2019-09-09 2021-08-26 한국교통대학교산학협력단 전기화학적 무선 진단이 가능한 탄소 양자점의 제조방법, 및 이를 활용한 전기화학적 무선진단방법
CN111562254A (zh) * 2020-05-27 2020-08-21 济南大学 一种基于功能化磁性二氧化硅的化学发光传感器的制备方法

Family Cites Families (14)

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Publication number Priority date Publication date Assignee Title
JP4274561B2 (ja) * 1997-08-01 2009-06-10 キヤノン株式会社 プローブアレイとその製造方法
SI1144685T1 (en) 1999-01-18 2003-10-31 Friz Biochem Gmbh Method for electrochemically detecting nucleic acid-oligomer hybridisation events
US6265170B1 (en) * 2000-01-24 2001-07-24 Ingeneus Corporation Homogenous assay of duplex of triplex hybridization by means of multiple measurements under varied conditions
JP2002286736A (ja) 2001-03-28 2002-10-03 Canon Inc プローブ担体の製造方法およびプローブ担体の製造装置
JP2004177399A (ja) 2002-11-14 2004-06-24 Toyama Prefecture ハイブリダイゼーションの検出方法
US20060275786A1 (en) 2003-03-05 2006-12-07 Yi-Tao Long Electrochemical detection of DNA binding
DE10311315A1 (de) 2003-03-14 2004-09-30 Apibio Sas Verfahren und Vorrichtung zur Detektion von Biomolekülen
WO2005017202A2 (en) 2003-05-13 2005-02-24 Gen-Probe Incorporated Method and kit for identifying antibiotic-resistant microorganisms
US8252533B2 (en) 2007-02-23 2012-08-28 Panagene Inc. Method for increasing efficiency or specificity of hybridization between PNA probes immobilized on support and target nucleic acids
KR101072900B1 (ko) 2007-12-04 2011-10-17 주식회사 파나진 고정화된 펩티드핵산 프로브를 사용한 표적핵산의 선택적 표지 및 검출 방법
GB0804491D0 (en) 2008-03-11 2008-04-16 Iti Scotland Ltd Detecting analytes
GB0805814D0 (en) * 2008-03-31 2008-04-30 Cambridge Entpr Ltd Biosensor for analyte detection
WO2010060060A1 (en) * 2008-11-24 2010-05-27 Adnavance Technologies, Inc. Electrochemical methods of detecting nucleic acid hybridization
JP2013513790A (ja) 2009-12-09 2013-04-22 アイティーアイ・スコットランド・リミテッド 検体の検出

Also Published As

Publication number Publication date
RU2014125212A (ru) 2015-12-27
GB201120118D0 (en) 2012-01-04
CN104145026A (zh) 2014-11-12
GB201212662D0 (en) 2012-08-29
US20150148257A1 (en) 2015-05-28
ZA201404463B (en) 2015-10-28
KR20140096104A (ko) 2014-08-04
EP2776583A1 (en) 2014-09-17
BR112014012045A2 (pt) 2017-05-30
WO2013076143A1 (en) 2013-05-30
US9777337B2 (en) 2017-10-03
AU2012342522A1 (en) 2014-05-29
JP2015503095A (ja) 2015-01-29
GB2496932A (en) 2013-05-29
IN2014CN03508A (es) 2015-10-09

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