MX2007013497A - Medicion de la actividad de trombina en la sangre entera. - Google Patents
Medicion de la actividad de trombina en la sangre entera.Info
- Publication number
- MX2007013497A MX2007013497A MX2007013497A MX2007013497A MX2007013497A MX 2007013497 A MX2007013497 A MX 2007013497A MX 2007013497 A MX2007013497 A MX 2007013497A MX 2007013497 A MX2007013497 A MX 2007013497A MX 2007013497 A MX2007013497 A MX 2007013497A
- Authority
- MX
- Mexico
- Prior art keywords
- sample
- thrombin
- layer
- whole blood
- thrombin activity
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/974—Thrombin
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Neurosurgery (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
La invencion se refiere a un metodo para determinar la actividad de trombina in vitro en una muestra, en donde la muestra es una muestra de sangre y la generacion de trombina se mide mediante los pasos de: - poner en contacto una capa de dicha muestra con un sustrato fuorogeno de trombina, en donde dicha capa tiene un espesor dentro de un rango de 0.05 a 5 mm, y una superficie dentro de un rango de 10 a 500 mm2; - permitir que se genere trombina en dicha muestra; - medir la fluorescencia emitida de la superficie de la capa, mediante el grupo fluorescente liberado del sustrato fluorogeno como resultado de la accion enzimatica de la trombina generada en dicho sustrato fluorogeno.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP05290952A EP1717588A1 (en) | 2005-04-29 | 2005-04-29 | Measuring thrombin activity in whole blood |
PCT/EP2006/004945 WO2006117246A1 (en) | 2005-04-29 | 2006-04-26 | Measuring thrombin activity in whole blood |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2007013497A true MX2007013497A (es) | 2008-04-04 |
Family
ID=34978991
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2007013497A MX2007013497A (es) | 2005-04-29 | 2006-04-26 | Medicion de la actividad de trombina en la sangre entera. |
Country Status (16)
Country | Link |
---|---|
US (1) | US8916356B2 (es) |
EP (2) | EP1717588A1 (es) |
JP (1) | JP4897794B2 (es) |
KR (1) | KR20080025043A (es) |
CN (1) | CN101171519A (es) |
AR (1) | AR053371A1 (es) |
AT (1) | ATE483984T1 (es) |
AU (1) | AU2006243283B2 (es) |
BR (1) | BRPI0612967B8 (es) |
CA (1) | CA2606906C (es) |
DE (1) | DE602006017350D1 (es) |
ES (1) | ES2354086T3 (es) |
MX (1) | MX2007013497A (es) |
RU (1) | RU2422835C2 (es) |
TW (1) | TW200716982A (es) |
WO (1) | WO2006117246A1 (es) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2654474A1 (en) * | 2006-06-06 | 2007-12-13 | Thrombinoscope B.V. | Method of determining enzymatic activity in biological media |
CN101548019A (zh) * | 2006-11-02 | 2009-09-30 | 凝血酶测量有限公司 | 在含有细胞的复合生物基质中测量瞬时蛋白水解活性浓度的方法 |
ATE516504T1 (de) | 2008-02-07 | 2011-07-15 | Synapse Bv | Um den einfluss von störfaktoren korrigierte messung des zeitlichen verlaufs einer enzymatischen aktivität bei der reaktion eines enzyms mit einem substrat |
FR2933496B1 (fr) * | 2008-07-02 | 2012-10-05 | Lfb Biotechnologies | Procede de mesure du taux de facteur vii active dans un echantillon |
CN102124346B (zh) * | 2008-07-16 | 2014-01-15 | 雷迪奥米特医学公司 | 凝血酶底物和测定样本中生物活性凝血酶水平的测定法 |
US8828730B2 (en) | 2008-08-05 | 2014-09-09 | Synapse B.V. | Method and assembly for measuring thrombin generation in plasma |
JP5582745B2 (ja) * | 2009-09-09 | 2014-09-03 | メタウォーター株式会社 | 化学発光反応の時間計測による被検出物質の濃度を測定する方法およびそれに使用するキット |
US8293493B2 (en) * | 2010-01-27 | 2012-10-23 | Adventist Health System/Sunbelt, Inc. | Thrombin generation determination method |
CN102798598A (zh) * | 2011-05-25 | 2012-11-28 | 兆科药业(合肥)有限公司 | 一种检测磷脂依赖性凝血因子x激活物酶活性的方法 |
RU2518247C2 (ru) * | 2011-07-26 | 2014-06-10 | Общество с ограниченной ответственностью "ГемаКор Лабс" (ООО "ГемаКор Лабс") | Способ определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе, устройство для реализации указанного способа и способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в гетерогенной системе |
BR112014003787A2 (pt) | 2011-08-23 | 2020-10-27 | Synapse B.V. | inibidores termoestáveis de ativação do sistema de coagulação sanguínea por contato com superfícies estranhas |
US10188302B2 (en) * | 2011-12-31 | 2019-01-29 | The University Of Vermont And State Agriculture College | Methods for dynamic visualization of clinical parameters over time |
KR101383333B1 (ko) * | 2012-01-13 | 2014-04-10 | 연세대학교 산학협력단 | 트롬빈 생성 측정 방법 |
EP2677037B1 (en) * | 2012-06-21 | 2016-04-13 | Synapse B.V. | Simultaneous measurement of thrombin generation and clot strength in plasma and whole blood |
WO2014098056A1 (ja) | 2012-12-18 | 2014-06-26 | 第一三共株式会社 | トロンビン産生の測定方法 |
CN103185710B (zh) * | 2013-02-04 | 2015-01-07 | 中国医学科学院输血研究所 | 人静脉注射免疫球蛋白中活化凝血因子ⅺ检测方法 |
CN104977259A (zh) * | 2014-04-04 | 2015-10-14 | 天津药物研究院 | 一种基于高通量检测的血小板聚集测定方法及在药物筛选中的应用 |
US9986941B2 (en) | 2014-06-26 | 2018-06-05 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and apparatus for rapid monitoring of hemostatic state |
CN104459165B (zh) * | 2014-12-10 | 2016-07-06 | 中国医学科学院输血研究所 | 一种检测人凝血酶原复合物抗凝能力的方法 |
US10633645B2 (en) * | 2015-12-30 | 2020-04-28 | General Electric Company | Calcium controlled activation of platelets via electrical stimulation |
US10794896B2 (en) | 2016-01-29 | 2020-10-06 | Sony Corporation | Blood coagulation system examination module, blood coagulation system examination system, blood coagulation system examination method, and determination method of parameter for blood coagulation system examination module |
EP3363912B1 (en) | 2017-02-17 | 2020-10-28 | Synapse B.V. | Self-calibrated assay for determining proteolytic enzyme generation, dedicated means and uses thereof |
CN107102125B (zh) * | 2017-04-17 | 2019-07-09 | 中国科学院苏州生物医学工程技术研究所 | 血栓弹力图仪的校准方法 |
RU2709341C1 (ru) * | 2019-04-19 | 2019-12-17 | федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр профилактической медицины" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ ПМ" Минздрава России) | Определение циркулирующего тромбина в тесте активации системы комплемента |
WO2021019529A1 (en) * | 2019-07-31 | 2021-02-04 | Tel Hashomer Medical Research Infrastructure And Services Ltd. | Methods and kits for identifying thrombus origin and optimizing post-ischemic event treatment |
CN110412001B (zh) * | 2019-08-01 | 2022-02-11 | 武汉塞力斯生物技术有限公司 | 一种动态监测凝血酶生成能力的试剂盒 |
CN110568117B (zh) * | 2019-09-19 | 2022-03-22 | 青岛海洋生物医药研究院股份有限公司 | 一种多靶点抗血栓活性物质的液相色谱-质谱筛选方法 |
WO2022137002A1 (en) | 2020-12-21 | 2022-06-30 | Ethicon, Inc. | Bleeding detection method |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS55164356A (en) * | 1979-06-08 | 1980-12-22 | Fuji Photo Film Co Ltd | Multi-layer analysis sheet for liquid sample analysis |
AU4287593A (en) * | 1992-04-27 | 1993-11-29 | Avocet Medical, Inc. | Test article and method for performing blood coagulation assays |
EP1504269B1 (en) * | 2002-05-01 | 2007-07-25 | Synapse B.V. | Diagnostic test for determining the concentration of transient proteolytic activity in composite biological media |
-
2005
- 2005-04-29 EP EP05290952A patent/EP1717588A1/en not_active Withdrawn
-
2006
- 2006-04-26 AT AT06753838T patent/ATE483984T1/de not_active IP Right Cessation
- 2006-04-26 BR BRPI0612967A patent/BRPI0612967B8/pt active IP Right Grant
- 2006-04-26 CN CNA2006800147599A patent/CN101171519A/zh active Pending
- 2006-04-26 RU RU2007137972/15A patent/RU2422835C2/ru not_active IP Right Cessation
- 2006-04-26 EP EP06753838A patent/EP1875249B1/en active Active
- 2006-04-26 US US11/919,431 patent/US8916356B2/en active Active
- 2006-04-26 KR KR1020077027433A patent/KR20080025043A/ko active IP Right Grant
- 2006-04-26 WO PCT/EP2006/004945 patent/WO2006117246A1/en active Application Filing
- 2006-04-26 MX MX2007013497A patent/MX2007013497A/es active IP Right Grant
- 2006-04-26 ES ES06753838T patent/ES2354086T3/es active Active
- 2006-04-26 AU AU2006243283A patent/AU2006243283B2/en not_active Ceased
- 2006-04-26 DE DE602006017350T patent/DE602006017350D1/de active Active
- 2006-04-26 CA CA2606906A patent/CA2606906C/en not_active Expired - Fee Related
- 2006-04-26 JP JP2008508173A patent/JP4897794B2/ja active Active
- 2006-04-28 TW TW095115377A patent/TW200716982A/zh unknown
- 2006-04-28 AR ARP060101736A patent/AR053371A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
DE602006017350D1 (de) | 2010-11-18 |
BRPI0612967B1 (pt) | 2021-02-02 |
US8916356B2 (en) | 2014-12-23 |
AU2006243283A1 (en) | 2006-11-09 |
EP1875249B1 (en) | 2010-10-06 |
US20090311730A1 (en) | 2009-12-17 |
TW200716982A (en) | 2007-05-01 |
KR20080025043A (ko) | 2008-03-19 |
CN101171519A (zh) | 2008-04-30 |
CA2606906C (en) | 2013-03-19 |
JP4897794B2 (ja) | 2012-03-14 |
AR053371A1 (es) | 2007-05-02 |
CA2606906A1 (en) | 2006-11-09 |
BRPI0612967A2 (pt) | 2010-12-14 |
RU2422835C2 (ru) | 2011-06-27 |
EP1875249A1 (en) | 2008-01-09 |
AU2006243283B2 (en) | 2011-06-30 |
RU2007137972A (ru) | 2009-06-10 |
EP1717588A1 (en) | 2006-11-02 |
ES2354086T3 (es) | 2011-03-09 |
JP2008539400A (ja) | 2008-11-13 |
WO2006117246A1 (en) | 2006-11-09 |
ATE483984T1 (de) | 2010-10-15 |
BRPI0612967B8 (pt) | 2021-07-27 |
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Legal Events
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FG | Grant or registration |