MC1785A1 - VECTORS FOR EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS - Google Patents
VECTORS FOR EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTSInfo
- Publication number
- MC1785A1 MC1785A1 MC86FR8600153D MC1785D MC1785A1 MC 1785 A1 MC1785 A1 MC 1785A1 MC 86FR8600153 D MC86FR8600153 D MC 86FR8600153D MC 1785 D MC1785 D MC 1785D MC 1785 A1 MC1785 A1 MC 1785A1
- Authority
- MC
- Monaco
- Prior art keywords
- hirudin
- secretion
- vectors
- expression
- transformed yeasts
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Tropical Medicine & Parasitology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Instructional Devices (AREA)
- Developing Agents For Electrophotography (AREA)
- Saccharide Compounds (AREA)
Abstract
Functional block of DNA enabling to prepare hirudine from yeast, characterized in that it comprises at least: - the gene of hirudine or one of its variants (gene H); - A DNA sequence (Str) comprising the signals providing for the transcription of the gene H by the yeast.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR8506672A FR2593518B1 (en) | 1985-05-02 | 1985-05-02 | VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS |
PCT/FR1986/000153 WO1986006406A1 (en) | 1985-05-02 | 1986-05-02 | Vectors for the expression and secretion of hirudin by transformed yeasts |
Publications (1)
Publication Number | Publication Date |
---|---|
MC1785A1 true MC1785A1 (en) | 1987-09-02 |
Family
ID=9318888
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MC86FR8600153D MC1785A1 (en) | 1985-05-02 | 1986-05-02 | VECTORS FOR EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS |
Country Status (23)
Country | Link |
---|---|
EP (1) | EP0200655B1 (en) |
JP (2) | JP2580141B2 (en) |
KR (1) | KR870700234A (en) |
AT (1) | ATE121778T1 (en) |
AU (1) | AU600667B2 (en) |
BG (1) | BG49717A3 (en) |
CA (1) | CA1341501C (en) |
CZ (1) | CZ282928B6 (en) |
DE (1) | DE3650306T2 (en) |
DK (1) | DK174837B1 (en) |
ES (1) | ES8703930A1 (en) |
FI (1) | FI104635B (en) |
FR (2) | FR2593518B1 (en) |
HK (1) | HK1006183A1 (en) |
HU (1) | HU202919B (en) |
IE (1) | IE67136B1 (en) |
MC (1) | MC1785A1 (en) |
NO (1) | NO302375B1 (en) |
PL (1) | PL155074B1 (en) |
PT (1) | PT82490B (en) |
RU (1) | RU1774950C (en) |
WO (1) | WO1986006406A1 (en) |
ZA (1) | ZA863261B (en) |
Families Citing this family (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3738541A1 (en) * | 1987-11-13 | 1989-05-24 | Hoechst Ag | METHOD FOR INSULATING AND CLEANING HIRUDINE |
FR2593518B1 (en) * | 1985-05-02 | 1989-09-08 | Transgene Sa | VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS |
MY101203A (en) * | 1985-12-12 | 1991-08-17 | Ucp Gen Pharma Ag | Production of thrombin iinhibitors. |
GB8530631D0 (en) | 1985-12-12 | 1986-01-22 | Ciba Geigy Ag | Thrombin inhibitors |
FR2607516B2 (en) * | 1986-12-01 | 1989-12-22 | Transgene Sa | IMPROVED VECTORS FOR EXPRESSING A HIRUDIN VARIANT IN YEAST, PROCESS AND PRODUCT OBTAINED |
MC1834A1 (en) * | 1986-07-10 | 1988-06-03 | Transgene Sa | DNA FUNCTIONAL BLOCK AND PLASMID ENCODING HIRUDINE, TRANSFORMED YEAST, PROCESS FOR PREPARING HIRUDINE, HIRUDINE OBTAINED AND ITS USE |
FR2607517B2 (en) * | 1986-12-01 | 1989-12-22 | Transgene Sa | VECTORS FOR EXPRESSING HIRUDIN VARIANTS IN YEAST, PROCESS AND PRODUCT OBTAINED |
FR2611723B1 (en) * | 1987-02-27 | 1989-09-08 | Transgene Sa | RECOMBINANT DNA EXPRESSION VECTOR ENCODING HIRUDINE, INFECTED OR TRANSFORMED MAMMALIAN CELL CULTURES, PROCESS FOR PREPARING HIRUDINE AND PROTEIN OBTAINED |
AU1987088A (en) * | 1987-06-24 | 1989-01-19 | Novo Nordisk A/S | A process for preparing a protein or polypeptide, a dna sequence coding for the polypeptide, a microorganism containing the dna sequence as well as the polypeptide and its use as a pharmaceutical preparation |
US5256559A (en) * | 1988-03-04 | 1993-10-26 | Biogen, Inc. | Methods and compositions for inhibiting platelet aggregation |
DE3819079A1 (en) * | 1988-06-04 | 1989-12-07 | Hoechst Ag | HIRUDINE DERIVATIVES WITH DELAYED EFFECT |
AU633020B2 (en) * | 1988-07-23 | 1993-01-21 | Novozymes Delta Limited | New secretory leader sequences |
DE3835815A1 (en) * | 1988-10-21 | 1990-04-26 | Hoechst Ag | NEW ISOHIRUDINE |
US5053453A (en) * | 1988-11-01 | 1991-10-01 | Baxter International Inc. | Thromboresistant materials and methods for making same |
CA2000887A1 (en) * | 1988-11-01 | 1990-05-01 | Cecilia S.L. Ku | Thromboresistant materials and methods for making same |
FR2646437B1 (en) * | 1989-04-28 | 1991-08-30 | Transgene Sa | NOVEL DNA SEQUENCES, THEIR APPLICATION AS A SEQUENCE ENCODING A SIGNAL PEPTIDE FOR THE SECRETION OF MATURE PROTEINS BY RECOMBINANT YEASTS, EXPRESSION CASSETTES, PROCESSED YEASTS AND PROCESS FOR PREPARING THE SAME |
DE4009268A1 (en) * | 1990-03-22 | 1991-09-26 | Consortium Elektrochem Ind | SECRETION OF HIRUDIN DERIVATIVES |
US6514730B1 (en) | 1991-03-21 | 2003-02-04 | Consortium für elektrochemische Industrie GmbH | Secretion of hirudin derivatives |
US5837808A (en) * | 1991-08-20 | 1998-11-17 | Baxter International Inc. | Analogs of hirudin |
DE4140381A1 (en) * | 1991-12-07 | 1993-06-09 | Hoechst Ag, 6230 Frankfurt, De | NEW SYNTHETIC ISOHIRUDINE WITH IMPROVED STABILITY |
ATE196927T1 (en) * | 1992-09-04 | 2000-10-15 | Novartis Ag | METHOD FOR PRODUCING PROTEASE INHIBITORS |
US6500645B1 (en) | 1994-06-17 | 2002-12-31 | Novo Nordisk A/S | N-terminally extended proteins expressed in yeast |
IL114160A (en) * | 1994-06-17 | 2006-12-31 | Novo Nordisk As | Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast |
DE19529997C1 (en) * | 1995-08-16 | 1997-04-10 | Hoechst Ag | A method for inactivating carboxypeptidase Y in hirudin-containing culture broths |
DE19543737A1 (en) | 1995-11-24 | 1997-05-28 | Hoechst Ag | Process for the ultrafiltration of biological matrices containing peptides or proteins |
DE19544233A1 (en) | 1995-11-28 | 1997-06-05 | Hoechst Ag | Process for using the yeast ADH II promoter system for the biotechnological production of heterologous proteins in high yields |
ZA9610456B (en) * | 1995-12-20 | 1997-06-20 | Novo Nordisk As | N-terminally extended proteins expressed in yeast |
DE69625623T2 (en) | 1996-01-31 | 2003-11-06 | Sumitomo Bakelite Co. Ltd., Tokio/Tokyo | Method of manufacturing semiconductor device encapsulated in epoxy resin |
US6265204B1 (en) | 1997-01-17 | 2001-07-24 | Genencor International, Inc. | DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi |
US7638618B2 (en) | 2001-02-20 | 2009-12-29 | Sanofi-Aventis Deutschland Gmbh | Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest |
US7202059B2 (en) | 2001-02-20 | 2007-04-10 | Sanofi-Aventis Deutschland Gmbh | Fusion proteins capable of being secreted into a fermentation medium |
DE10149678A1 (en) | 2001-10-09 | 2009-03-12 | Novel Science International Gmbh | New peptide compounds containing upto 6 aminoacid residues, are potent and specific thrombin inhibitors used for treating and diagnosing diseases associated with thrombin |
DE10301255A1 (en) | 2003-01-15 | 2004-07-29 | Cpi Creative Pharma International Gmbh | Organic compounds with biological action as thrombin inhibitors and their use |
US7795205B2 (en) | 2004-04-12 | 2010-09-14 | Canyon Pharmaceuticals, Inc. | Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0116201B1 (en) * | 1983-01-12 | 1992-04-22 | Chiron Corporation | Secretory expression in eukaryotes |
AU572125B2 (en) * | 1983-03-17 | 1988-05-05 | Mabco Limited | Monoclonal antibodies with specificity for crosslinked fibrin and their diagnotic uses |
JPS60501140A (en) * | 1983-04-22 | 1985-07-25 | アムジエン | Secretion of exogenous polypeptides by yeast |
JPS6041487A (en) * | 1983-04-25 | 1985-03-05 | ジエネンテツク・インコ−ポレイテツド | Use of alpha factor arrangement in yeast development system |
NZ207926A (en) * | 1983-04-25 | 1988-04-29 | Genentech Inc | Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast |
JPS59227899A (en) * | 1983-05-25 | 1984-12-21 | チロン・コ−ポレイシヨン | Manufacture of growth hormone release factor |
CA1341414C (en) * | 1984-03-27 | 2002-12-31 | Paul Tolstoshev | Expression vectors of hirudine, transformed cells and process for the preparation of hirudine |
DE3583361D1 (en) * | 1984-06-14 | 1991-08-08 | Ucp Gen Pharma Ag | METHOD FOR PRODUCING THROMBIN INHIBITORS. |
DE3429430A1 (en) * | 1984-08-10 | 1986-02-20 | Hoechst Ag, 6230 Frankfurt | GENE TECHNOLOGICAL METHOD FOR PRODUCING HIRUDINE AND MEANS FOR IMPLEMENTING THIS METHOD |
DE3445517C2 (en) * | 1984-12-13 | 1993-11-18 | Ciba Geigy | DNA sequence coding for a hirudin-like protein and method for producing a hirudin-like protein |
FR2593518B1 (en) * | 1985-05-02 | 1989-09-08 | Transgene Sa | VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS |
-
1985
- 1985-05-02 FR FR8506672A patent/FR2593518B1/en not_active Expired
-
1986
- 1986-04-30 CA CA000508053A patent/CA1341501C/en not_active Expired - Fee Related
- 1986-04-30 PT PT82490A patent/PT82490B/en unknown
- 1986-04-30 ZA ZA863261A patent/ZA863261B/en unknown
- 1986-05-02 IE IE118386A patent/IE67136B1/en not_active IP Right Cessation
- 1986-05-02 MC MC86FR8600153D patent/MC1785A1/en unknown
- 1986-05-02 AT AT86400961T patent/ATE121778T1/en not_active IP Right Cessation
- 1986-05-02 WO PCT/FR1986/000153 patent/WO1986006406A1/en active IP Right Grant
- 1986-05-02 ES ES555121A patent/ES8703930A1/en not_active Expired
- 1986-05-02 JP JP61502537A patent/JP2580141B2/en not_active Expired - Lifetime
- 1986-05-02 AU AU57787/86A patent/AU600667B2/en not_active Expired
- 1986-05-02 EP EP86400961A patent/EP0200655B1/en not_active Expired - Lifetime
- 1986-05-02 PL PL1986259298A patent/PL155074B1/en unknown
- 1986-05-02 KR KR860700971A patent/KR870700234A/en not_active Application Discontinuation
- 1986-05-02 DE DE3650306T patent/DE3650306T2/en not_active Expired - Lifetime
- 1986-05-02 HU HU863185A patent/HU202919B/en unknown
- 1986-05-04 CZ CS863217A patent/CZ282928B6/en not_active IP Right Cessation
- 1986-07-10 FR FR868610090A patent/FR2601383B2/en not_active Expired
- 1986-12-19 BG BG77615A patent/BG49717A3/en unknown
- 1986-12-23 FI FI865303A patent/FI104635B/en not_active IP Right Cessation
- 1986-12-30 NO NO865336A patent/NO302375B1/en not_active IP Right Cessation
- 1986-12-30 RU SU864028760A patent/RU1774950C/en active
- 1986-12-30 DK DK198606343A patent/DK174837B1/en not_active IP Right Cessation
-
1996
- 1996-03-26 JP JP8070208A patent/JP2779930B2/en not_active Expired - Lifetime
-
1998
- 1998-06-12 HK HK98105246A patent/HK1006183A1/en not_active IP Right Cessation
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
FR2593518B1 (en) | VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS | |
ES2056817T5 (en) | TRANSFORMATION OF TRICHODERMA. | |
MA21169A1 (en) | DEOXYRIBONUCLEIC ACIDS AND PROCESS FOR THE PREPARATION OF PROTEINS DERIVED THEREFROM. | |
GR78180B (en) | ||
DE3863996D1 (en) | ALCOHOL-FREE BEER. | |
ES2108681T3 (en) | EXPRESSION OF HUMAN SERUM ALBUMIN IN METHYLTROPHIC YEAST. | |
ZW2485A1 (en) | Cloning of dna for protozoal antigens | |
FI90667C (en) | The method produces a novel human type I interferon protein | |
FR2607517B2 (en) | VECTORS FOR EXPRESSING HIRUDIN VARIANTS IN YEAST, PROCESS AND PRODUCT OBTAINED | |
IT8883551A0 (en) | PROCEDURE FOR THE EXTRACTION AND PURIFICATION OF DNA | |
ES2078229T3 (en) | PROCEDURE FOR THE PREPARATION OF A POLYPEPTIDE SIMILAR TO MOTILINE AND ITS EXPRESSION. | |
AU553409B2 (en) | Hybrid vectors and process for improving the amplification and expression of hybrid vectors by use of mitochondrial dna | |
PT87332A (en) | METHOD OF PURIFICATION OF PRODUCT OF EXPRESSION OF RECOMBINANT FERMENT | |
EP0285361A3 (en) | Purification of apase-1 1 and retrieval of the nematode resistance gene | |
ES2101694T3 (en) | EXPRESSION OF PROTEINIC SWEETENERS IN YEAST. | |
ATE119206T1 (en) | DMD PROBE. | |
IT8521507A0 (en) | CLONING VECTOR, RECOMBINANT DNA MOLECULES, STRAINS OF BACILLUS SUBTILIS TRANSFORMED WITH THE SAID MOLECULES AND METHODS FOR THE EXPRESSION OF HETEROLOGOUS GENES AND PRODUCTION AND SECRETION OF PROTEINS CODED BY THE SAID GENES. | |
IT8621735A0 (en) | MICROORGANISCMS MANIPULATED WITH GENETIC ENGINEERING MEANS FOR THE PRODUCTION OF L-AMINO ACIDS. | |
Kriss | p. 103, the last line of the section entitled 5'-Nucleotidase assay should read:/~ mol phosphate released/h per mg protein. | |
IT8404989V0 (en) | JOINT FOR THE TILTING OF AFT WHEELS FOR THE TOWING OF TIRES | |
TH22943A (en) | Recombinant, a protein defining DEV-1 useful for HIV detection, DNA sequencing, protein coding, and immunological analysis using this protein. | |
IT8641582A0 (en) | PROCEDURE AND SYSTEM FOR THE SEPARATION OF PROTEINS FROM LIME WATER AND THE ELIMINATION OF SULPHIDE. | |
ATE84315T1 (en) | RECOMBINANT DNA FOR THE REPLIABLE AND INDUCABLE EXPRESSION OF FOREIGN GENES. |