MA43987A - Détermination de la pureté de protéine multimère - Google Patents

Détermination de la pureté de protéine multimère

Info

Publication number
MA43987A
MA43987A MA043987A MA43987A MA43987A MA 43987 A MA43987 A MA 43987A MA 043987 A MA043987 A MA 043987A MA 43987 A MA43987 A MA 43987A MA 43987 A MA43987 A MA 43987A
Authority
MA
Morocco
Prior art keywords
purity
determination
multimer protein
multimer
protein
Prior art date
Application number
MA043987A
Other languages
English (en)
Inventor
Jiann-Kae Luo
Kathir Muthusamy
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MA43987A publication Critical patent/MA43987A/fr

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854Immunoglobulins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46Hybrid immunoglobulins
    • C07K16/468Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/33Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/30Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52Constant or Fc region; Isotype
    • C07K2317/526CH3 domain
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00Features of devices classified in G01N21/00
    • G01N2201/06Illumination; Optics
    • G01N2201/061Sources
    • G01N2201/06113Coherent sources; lasers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44717Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44756Apparatus specially adapted therefor
    • G01N27/44795Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
MA043987A 2015-05-12 2016-05-11 Détermination de la pureté de protéine multimère MA43987A (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562160341P 2015-05-12 2015-05-12

Publications (1)

Publication Number Publication Date
MA43987A true MA43987A (fr) 2018-12-19

Family

ID=56072452

Family Applications (2)

Application Number Title Priority Date Filing Date
MA043987A MA43987A (fr) 2015-05-12 2016-05-11 Détermination de la pureté de protéine multimère
MA056416A MA56416A (fr) 2015-05-12 2016-05-11 Détermination de la pureté de protéines multimériques

Family Applications After (1)

Application Number Title Priority Date Filing Date
MA056416A MA56416A (fr) 2015-05-12 2016-05-11 Détermination de la pureté de protéines multimériques

Country Status (11)

Country Link
US (2) US10520511B2 (fr)
EP (2) EP3885368A1 (fr)
JP (3) JP7240812B2 (fr)
AR (1) AR104610A1 (fr)
AU (2) AU2016261854B2 (fr)
CA (1) CA2984010A1 (fr)
ES (1) ES2881359T3 (fr)
HK (1) HK1252480A1 (fr)
MA (2) MA43987A (fr)
PL (1) PL3294775T3 (fr)
WO (1) WO2016183222A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3384274B1 (fr) 2015-11-30 2021-11-10 Intabio, Inc. Procédés de caractérisation d'échantillon
CN109937034B (zh) 2016-11-21 2022-09-16 济世-伊沃泰克生物制品有限公司 阿柏西普制剂及其用途
EP3668985B1 (fr) 2017-08-17 2021-06-16 Just-Evotec Biologics, Inc. Procédé de purification de protéine glycosylée à partir de galectines et d'autres contaminants de cellules hôtes
JP7333326B2 (ja) 2018-01-29 2023-08-24 インタバイオ, エルエルシー 試料の特性評価のための装置、方法およびキット
WO2019232397A1 (fr) 2018-05-31 2019-12-05 Intabio, Inc. Logiciel pour systèmes microfluidiques faisant interface avec la spectrométrie de masse
MX2021002573A (es) * 2018-09-10 2021-05-12 Genentech Inc Sistemas y procedimientos para electroforesis capilar de afinidad.
US11573226B2 (en) * 2018-09-10 2023-02-07 Genentech, Inc. Systems and methods for affinity capillary electrophoresis
US11782023B2 (en) * 2018-12-19 2023-10-10 Regeneron Pharmaceuticals, Inc. Ce-western applications for antibody development
KR20210122803A (ko) * 2019-01-31 2021-10-12 리제너론 파아마슈티컬스, 인크. 항체 전하 이질성의 고유 마이크로유체 ce-ms 분석
US11285484B2 (en) 2019-08-12 2022-03-29 Intabio, Llc Multichannel isoelectric focusing devices and high voltage power supplies

Family Cites Families (22)

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AUPP385598A0 (en) 1998-06-02 1998-06-25 Life Therapeutics Limited Purification of antibodies
US6472179B2 (en) * 1998-09-25 2002-10-29 Regeneron Pharmaceuticals, Inc. Receptor based antagonists and methods of making and using
EP1194768A4 (fr) * 1999-04-20 2008-03-26 Target Discovery Inc Procedes de cartographie peptidique de polypeptides, etablissement de profils et systeme de base de donnees bioinformatique
US6818112B2 (en) * 1999-04-20 2004-11-16 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US7087411B2 (en) 1999-06-08 2006-08-08 Regeneron Pharmaceuticals, Inc. Fusion protein capable of binding VEGF
AUPQ743300A0 (en) * 2000-05-11 2000-06-01 Life Therapeutics Limited Separation of avian antibodies
US6927004B2 (en) 2002-03-08 2005-08-09 Asml Netherlands B.V. Mask for use in lithography, method of making a mask, lithographic apparatus, and device manufacturing method
JPWO2007034907A1 (ja) * 2005-09-22 2009-03-26 財団法人化学物質評価研究機構 被検化学物質の生物に与える影響の予測方法
EP1803814A1 (fr) 2005-12-27 2007-07-04 SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A. Procédé d'amélioration la capacité de sélection d'anticorps dans une bibliothèque d'affichage de phages
EP4218801A3 (fr) * 2006-03-31 2023-08-23 Chugai Seiyaku Kabushiki Kaisha Procédé de modification d'anticorps pour purifier un anticorps bispécifique
US7771997B2 (en) 2007-06-04 2010-08-10 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
PL2202245T3 (pl) * 2007-09-26 2017-02-28 Chugai Seiyaku Kabushiki Kaisha Sposób modyfikowania punktu izoelektrycznego przeciwciała poprzez podstawienie aminokwasu w cdr
JP4977588B2 (ja) 2007-12-07 2012-07-18 富士フイルム株式会社 イムノクロマトグラフ方法
KR20120027055A (ko) 2009-06-26 2012-03-20 리제네론 파라마큐티칼스 인코포레이티드 천연 면역글로불린 포맷을 가지는 용이하게 분리된 이중특이성 항체
SG195073A1 (en) 2011-05-21 2013-12-30 Macrogenics Inc Deimmunized serum-binding domains and their use for extending serum half-life
GB2504139B (en) * 2012-07-20 2014-12-31 Argen X Bv Antibodies to highly conserved targets produced by the immunisation of Camelidae species
EP2604624A1 (fr) * 2011-12-13 2013-06-19 University College Dublin National University Of Ireland, Dublin Peptides de liaison de l'inhibiteur de protéase de Bowman-Birk (BBO) et utilisations associées pour la séparation, la détection ou la purification du BBI.
US9815909B2 (en) * 2012-03-13 2017-11-14 Novimmune S.A. Readily isolated bispecific antibodies with native immunoglobulin format
SG11201407644UA (en) 2012-06-05 2014-12-30 Regeneron Pharma Methods for making fully human bispecific antibodies using a common light chain
TWI675044B (zh) * 2012-11-14 2019-10-21 美商再生元醫藥公司 重組細胞表面捕捉蛋白質
WO2015024896A1 (fr) * 2013-08-19 2015-02-26 F. Hoffmann-La Roche Ag Séparation d'anticorps bispécifiques et sous-produits de la production d'anticorps bispécifiques utilisant la chromatographie sur hydroxyapatite
CA2922979A1 (fr) * 2013-09-03 2015-03-12 Novimmune S.A. Molecules de liaison bispecifiques facilement isolees au format natif, mais possedant des regions constantes mutees

Also Published As

Publication number Publication date
JP7240812B2 (ja) 2023-03-16
AU2022203033A1 (en) 2022-05-26
HK1252480A1 (zh) 2019-05-24
EP3294775A1 (fr) 2018-03-21
US20170045527A1 (en) 2017-02-16
US10520511B2 (en) 2019-12-31
JP2022171730A (ja) 2022-11-11
US20200166522A1 (en) 2020-05-28
WO2016183222A1 (fr) 2016-11-17
EP3885368A1 (fr) 2021-09-29
AU2016261854A1 (en) 2017-11-09
WO2016183222A4 (fr) 2017-01-05
JP2018528389A (ja) 2018-09-27
MA56416A (fr) 2022-05-04
ES2881359T3 (es) 2021-11-29
JP2021152544A (ja) 2021-09-30
AU2016261854B2 (en) 2022-05-26
EP3294775B1 (fr) 2021-07-14
JP7137663B2 (ja) 2022-09-14
PL3294775T3 (pl) 2021-12-13
CA2984010A1 (fr) 2016-11-17
AR104610A1 (es) 2017-08-02

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