LV11269B - Method of separation fat tissue from body and the device of doing thereof - Google Patents
Method of separation fat tissue from body and the device of doing thereof Download PDFInfo
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- LV11269B LV11269B LV940244A LV940244A LV11269B LV 11269 B LV11269 B LV 11269B LV 940244 A LV940244 A LV 940244A LV 940244 A LV940244 A LV 940244A LV 11269 B LV11269 B LV 11269B
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Abstract
Description
Izgudrojums attiecas uz medicīnu, tieši uz ķirurģiju, transplantalogiju un citlogiju un skar audu atdalīšanas metožu izstrādes jautājumu, audu dzīvotspējas saglabāšanu vēlākai transplantācijai un implantācijai.The invention relates to medicine, specifically to surgery, transplantalogy and cytology, and concerns the development of methods of tissue separation, the maintenance of tissue viability for subsequent transplantation and implantation.
No organisma atdalītu audu dzīvotspējas saglabāšana ir svarīgs jautājums audu un orgānu veiksmīgas transplantācijas un implantācijas iespēju pētīšanā, kuras nepieciešamas dažādu organisma defektu korekcijas veikšanai.Maintaining the viability of tissues removed from the body is an important issue in exploring the potential for successful transplantation and implantation of tissues and organs needed to correct various defects in the body.
Atdalāmo audu dzīvotspējas saglabāšana tieši atkarīga no to atdalīšanas paņēmiena.The maintenance of the viability of the tissues to be removed depends directly on the method of separation.
Ir zināms paņēmiens cilvēkā ādas fibroplastu iegūšanai ādas paraugu apstrādes ceļā, 15 min. apdedzinot ar 0,25 7. tripsīna šķīdumu 20° C (i).There is a known technique for obtaining human skin fibroplasts by skin sample processing, 15 min. firing with 0.25 7 trypsin solution at 20 ° C (i).
Sīs paņēmiens ir izgudrojuma analogs.This technique is analogous to the invention.
Transplantāts no kultivētiem cilvēka al1ofibroplastiem tiek pielietots apdeguma brūču nosegšanai. Tomēr aprakstīta metode nav derīga taukaudu atdalīšanai.The graft from cultured human allofibroplasty is used to cover burn wounds. However, the method described is not valid for the removal of adipose tissue.
Ir zināma audu atdalīšanas metode, piemēram, taukaudu - ādas un zemādas saistaudu atdalīšanas ceļā.There is a known method of tissue separation, for example, by removing fatty tissue from the skin and subcutaneous connective tissue.
Ar šo metodi atdalot tauku audus - pēc ādas pārgriešanas izskrāpē zemādas saistaudus ar speciālas ierīces palīdzību un pēc tam izdara aktīvu aspirāciju <2>. Minētais paņēmiens un ierīce ir izgudrojuma prototipi. Tomēr iegūstot taukaudus pēc prototipa metodes, jāatzīmē stipra šūnu un audu traumēšanās, sevišķi tiek traumēti asinsvadi, nervu gali un zarojums. Atdalot tauku audus pēc prototipa notiek spēcīga asiņošana un kā sekas jāmin jūtīguma zušana un ādas trofikas pasliktināšanās. Pec prototipa iegūtie taukaudi ir nekrotiski, šūnu apvalks un mambrāna tiek bojāti, taukaudu šūnas zaudē dzīvotspēju un pārvēršas salipušā bezformas masā.This method removes adipose tissue by scraping the subcutaneous connective tissue with a special device after cutting the skin and then performing an active aspiration <2>. The said method and device are prototypes of the invention. However, when obtaining adipose tissue by the prototype method, significant cell and tissue trauma should be noted, especially blood vessels, nerve endings and branching. Separation of adipose tissue by prototype results in heavy bleeding and loss of sensitivity and worsening of skin trophies. The adipose tissue obtained after the prototype is necrotic, the cell shell and the mammary membrane are damaged, the adipose tissue cells lose their vitality and turn into an adherent formless mass.
Izgudrojuma mērķis ir traumēšanas samazināšana, asinsvadu un nervu veseluma saglabāšana aperācijas procesā, asins zuduma samazināšana un tauku šūnu apvalku un membrānu veseluma saglabāšana, taukaudu dzīvotspējas saglabāšana.The object of the invention is to reduce trauma, to maintain the integrity of blood vessels and nerves during the aeration process, to reduce blood loss and to maintain the integrity of fat cell envelopes and membranes, and to maintain the vitality of adipose tissue.
Uzstādītais mērķis izstrādājot paņēmienu sasniedzams uz tā rēķina, ka atdalot taukaudus no organisma iepriekš zem ādas ievada šķīdumu, kurš emulgē tauku audus, attiecībās pret atdalāmo audu apjomu 1:(47-6) 30-40 min. laikā, pēc tam šķīdumu 30-40 min. laikā ievada tauku audu biezākajos slānos, izdara dūrienu un atdala audus aktīvas aspirācijas ceļā.The aim is achieved by developing a technique that removes the adipose tissue from the body by subcutaneous injection of a solution which emulsifies the adipose tissue in a ratio of 1: (47-6) 30-40 min. time, then the solution for 30-40 min. During the course of the procedure, the fat is deposited in the thicker layers of the tissue, the stitch is made and the tissue is removed by active aspiration.
Lai realizētu paņēmienu radīta ierīce, izpildīta caurulītes veidā ar slēgplāksni nslēgtu gala virsmu un rindu atvērumu uz tās virsmas, pie tam papildus uz caurulītes virsmas no distālā gala puses iegravēta grope 10-15 mm garumā ar platumu ne lielāku par caurulītes diametru un dažiem caururbtiem atvērumiem 0,3-1,0 mm diametrā novirzītiem attiecībā vienam pret otru.To implement the method, a device is made in the form of a tube with a locking plate with a locked end surface and rows of openings on its surface, additionally engraved on the surface of the tube at a distance of 10-15 mm with a width not exceeding the diameter of the tube , With a diameter of 3-1.0 mm directed towards each other.
Iesniedzējs nav atradis risinājumus par paņēmienu un ierīci, saturošus pazīmju kopumu un atšķirību pazīmes, tātad minētais risinājums ir jauninājums un atbilst kritērijam būtiskas atšķirības .The applicant has not found a solution for a method and device containing a set of features and differences, so this solution is an innovation and fulfills the criterion with significant differences.
Izgudrojumu paskaidro rasējums, kur zīm. i parādīts iesniedzamās ierīces kopējais izskats.The invention is explained by a drawing, wherein FIG. i shows the overall appearance of the device being submitted.
Piedāvājamā ierīce nodrošina iesniedzamā paņēmiena izpildi. Ierīce taukaudu atdalīšanai risināta kā caurulīte ar slēgplāksni noslēgtu gala virsmu, kuras proksimālais gals i pievienots atsūkšnas sistēmai (rasējumā nav parādīts). Ar slēgplāksni noslēgtā caurulītes gala virsma distālajā galā nosprostota un noapaļota. Uz cilindriskās virsmas iegravēta grope 2 10-15 mm garumā ar platumu ne lielāku par caurulītes diametru. Caurulītes diametrs priekš dažādām ķermeņa dalām svārstās no 3 līdz 6 mm. Lai uzlabotu taukaudu atsūkšanu aiz gropes uz caurulītes virsmas 45-50 mm garumā izvietoti vairākās rindās caururbti atvērumi 3 ar 0,3-1,0 mm diametru. Lai samazinātu trumēšanu caurulītes sieniņa, kura veido gropi pa tās perimetru noapaļota.The proposed device implements the method to be submitted. The device for the removal of fat tissue is arranged as a tube with a locking plate end surface, the proximal end of which is connected to the suction system (not shown in the drawing). The end face of the tube sealed with the locking plate at the distal end is blocked and rounded. A groove 2 10-15 mm long with a width not exceeding the diameter of the tube is engraved on the cylindrical surface. The diameter of the tube for various body parts ranges from 3 to 6 mm. To improve liposuction beyond the groove, openings 3 with a diameter of 0.3-1.0 mm are arranged in several rows 45 to 50 mm long on the surface of the tube. To minimize drumming, the tube wall, which forms a groove around its perimeter, is rounded.
Paņēmienu īsteno ar ierīces palīdzību sekojošā veidā.The method is implemented by means of a device as follows.
Operāciju izdara ar vietējo anestēziju. Nosaka ķermeņa zonu, no kuras tiks atdalīti tauku audi. Ievada zem ādas šķīdumu, kurš emul gē tauku audus, attiecībās pret atdalāmo audu apjomu l:(4r<5)The operation is performed under local anesthesia. Determines the area of the body from which adipose tissue will be removed. Administer subcutaneous solution which emulsifies adipose tissue in proportion to the volume of tissue removed l: (4r <5)
30-40 min. laikā. Pēc tam emulgējošo sastāvu ievada tauku audu biezākajos slāņos 30-40 min. laikā. Izdara dūrienu un ievada caurulīti zem ādas tās vajadzīgajā garumā. Pēc tam ierīci pieslēdz atsūkšanas sistēmai. Taukaudu emulsija caur gropi 2 nonāk cauru lītes iekšienē un tiek ivadīta pa to savākšanas tilpnē. Pakāpeniski caurulīti ievada dziļāk. Uz caurulītes esošie caururbtie atvērumi 3, sekojoši viens aiz otra, nodrošina emulgēto tauku au du atsūkšanas hidrauliskus priekšnosacījumus. Gropes sieniņu un slēgplāksnes noapaļotās daļas samazina audu traumēšanu ierīces darba laikā.30-40 min. at a time. The emulsifying composition is then applied to the thicker layers of adipose tissue for 30-40 min. at a time. Make a stitch and insert the tube under the skin into the required length. The device is then connected to a suction system. The adipose tissue emulsion passes through the groove 2 into the interior of the teat and is introduced into the collecting vessel. Gradually the tube is inserted deeper. The drilled holes 3 on the tube, one after the other, provide the hydraulic prerequisites for the extraction of emulsified fat tissue. The rounded sections of the groove walls and locking plate reduce tissue injury during operation.
Pēc tauku audu atdalīšanas, caurulīti izņem no organisma. Uz dūrienā vietu uzliek kosmētisku šuvi.After the fat tissue is removed, the tube is removed from the body. A cosmetic seam is applied to the stitch.
Ar iesniegtā paņēmiena un ierīces palīdzību no organisma atdalītie tauku audi saglabā savu dzīvotspēju un tas atļauj tas turp māk efektīvi izmantot implantācijai defekktu koriģēšanai kosmeti skās operācijās u.c.By the method and device provided, the adipose tissue removed from the body retains its vitality and allows it to continue to be used effectively for implantation to correct defects in cosmetic operations, etc.
Izpētot atdalītos audus histoloģiski, atklāts: taukaudu šūnas pielīp cita citai un ir atdalītas kā tieva kolagēna šķiedra (krā seti ar hematoksi1īnu un eozīnu).Histological examination of the detached tissues revealed that adipose tissue cells adhere to each other and are separated as thin collagen fibers (hematoxin and eosin dyes).
Izplatījumā starp tauku šūnām sastopami fibroplasti un limfocīti. Tauku šūnu kodols bez hromatolīzes un karioreksis pazīmēmFibroplasts and lymphocytes are found in the distribution between fat cells. Fat cell nucleus without signs of chromatolysis and cariorexis
Šauras citoplazmas šūnas homogēni iekrāsojas. Plazmatisko membrānu defekti nav novēroti. Preparāts ir dzīvotspējīgi tauku au di bez distrofisku izmaiņu pazīmēm.Narrow cytoplasmic cells are homogeneously stained. No defects in plasma membranes have been observed. The product is a viable fatty tissue without signs of dystrophic changes.
Tātad izstrādātie paņēmiens un ierīce atļauj atdalīt dzīvotspē jigus tauku audus, kuri sastāv no tauku šūnu homogēna maisījuma, kur sūnas atdalītas viena no otras tievām kolagēnām šķiedrām.Thus, the method and device developed allow the separation of viable adipose tissue consisting of a homogeneous mixture of fat cells, where the moss is separated from each other by thin collagen fibers.
Paņēmiens un ierīce praktiski ļauj likvidēt organisma audu traumēšanu un izvairīties no asiņošanas, atdalot tauku audus.The method and device practically allow to eliminate trauma to the body's tissues and to avoid bleeding by removing fatty tissue.
-ό—-ό—
IZMANTOTA LITERATŪRA.LITERATURE USED.
1. D.S.Sarkisovs, Α.A,Alekseejevs u.c. Apdegumu arstēēana, izmantojot kultivētas cilvēka ādas āūnas. Lirurgija, 1993, 3, 22-27.1. D.S.Sarkisov, Α.A, Alexeyev, etc. Treatment of burns using cultured human skin. Surgery 1993, 3, 22-27.
2. Instrumentu katalogs. Firma Aesculap-Werke AG. Deutchland, 1992 - prototips.2. Tool Catalog. Aesculap-Werke AG. Deutchland, 1992 - Prototype.
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LV940244A LV11269B (en) | 1994-12-09 | 1994-12-09 | Method of separation fat tissue from body and the device of doing thereof |
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LV940244A LV11269B (en) | 1994-12-09 | 1994-12-09 | Method of separation fat tissue from body and the device of doing thereof |
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LV11269A LV11269A (en) | 1996-06-20 |
LV11269B true LV11269B (en) | 1996-12-20 |
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