LV11038B - Saf polypeptide, gene - Google Patents
Saf polypeptide, gene Download PDFInfo
- Publication number
- LV11038B LV11038B LVP-95-230A LV950230A LV11038B LV 11038 B LV11038 B LV 11038B LV 950230 A LV950230 A LV 950230A LV 11038 B LV11038 B LV 11038B
- Authority
- LV
- Latvia
- Prior art keywords
- gene
- ser
- ala
- saf
- vector
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Claims (20)
- LV 11038 Izgudrojuma formula 1. Būtībā tīrs polipeptīds ar šādu aminoskābju secību: Met Cys Ser Ala Vai Vai Ala Ala Ala Vai Ser Ser Met Ser Arg Arg Arg Arg Arg Ala Ser Ala Thr Arg Arg Ser Ala Ala Vai Ser Pro Pro His Thr Pro Tyr Gly Ser Gly Cys lle Ser Ala Cys Ser Trp Hys Ser Thr lle Thr Gly His Arg Ala Gln Thr Arg Leu Ala Ala Ser Ser Arg Ala Ser Arg Ala Ala Vai Gly Ser Phe Asp Gly Ala Lys Asn Arg Pro Ala Ser Ser Arg Arg Gln Ala Ala Ser Gly Cys Gln Gly Pro Ala Ser Ser Ser Arg Ala Gly Vai Arg Trp Ala Gly Thr Ala Ala Asn Gly Arg Gly Ser un/vai tā fragmenti ar sekundārā metabolismu aktivējošā faktora aktivitāti.
- 2. DNS secība, kas kodē polipeptīdu saskaņā ar 1. punktu.
- 3. DNS secība, kurā ietverta šāda nukleotīdu secība: ATG TGC TCG GCC GTC GTC GCG GCG GCC GTG AGC TGC ATG TCC CGC CGT CGC CGC CGG GCC AGC GCC ACC CGG CGC TCC GCT GGG GTG AGC CCG CCC CAC ACT CCG TAC CGC TCG GGC TGT ACT AGC GCG TGC TCC TGG CAC TCC ACC ATC ACC GGA CAC CGG GCA CAG ACC CGC TTG GCC GCC TCC TCG CGG GCC AGC CGG GCG GCG GTC GGC TCC TTC GAC GGG GCG AAG AAC AGG CCG GCC TCG TCG CGG CGG CAG GCC GCA TCG GAA TCG CAG GGG CCC GCG TCG TCC TCC CGT GCG GGG GTC CGC TGG GCC GGA ACG GCG GCG AAC GGC AGA GGC TGA.
- 4. DNS secība, kas hibridizējas ar nukleotīdu secību saskaņā ar 3. punktu, un kas kodē SAF tipa polipeptīdu.
- 5. Vektors, kas ietver DNS secību saskaņā ar 2. punktu.
- 6. Vektors saskaņā ar 5. punktu, kurā DNS secība atrodas starp regulējošām secībām.
- 7. Vektors saskaņā ar 6. punktu, kurā regulējošais apgabals atrodas pretstraumes virzienā no ATG kodona un tam ir šāda nukleotīdu secība: 1 AGATCTCCTCGTCCCACCGGCTGTCGAAGCTCCGCGCCTACAGCGCCAT CGACTTCGACCGGGAAATAGGAAGCGGCCGGCGACAAAACGGGGAGG GGCGGGAAACGTGGTCCGTTTCCCGCCCCTGCCCGTAGGCCGTGCGCG TCCCGCCGGTGCGCGGCGTTCAGCCCGCCGCCGCT.
- 8. Streptomyces saimniekšūna, kas transformēta ar vektoru saskaņā ar 5. punktu.
- 9. Paņēmiens polipeptīda iegūšanai, kas paredz kultivēt saimniekšūnu saskaņā ar 8. punktu.
- 10. Vektors, kas satur DNS secību saskaņā ar 3. punktu.
- 11. Vektors saskaņā ar 10. punktu, kurā DNS secība atrodas starp regulējošām secībām.
- 12. Vektors saskaņā ar 11. punktu, kurā regulējošais apgabals atrodas pretstraumes virzienā no ATG kodona un tam ir šāda nukleotīdu secība: AGATCTCCTCGTCCCACCGGCTGTCGAAGCTCCGCGCCTACAGCGCCAT CGACTTCGACCGGGAAATAGGAAGCGGCCGGCGACAAAACGGGGAGG GGCGGGAAACGTGGTCCGTTTCCCGCCCCTGCCCGTAGGCCGTGCGCG TCCCGCCGGTGCGCGGCGTTCAGCCCGCCGCCGCT.
- 13. Streptomyces saimniekšūna, kas transformēta ar vektoru saskaņā ar 10. punktu.
- 14. Paņēmiens polipeptīda iegūšanai, kas paredz saimniekšūnas saskaņā ar 13. punktu kultivēšanu.
- 15. Vektors, kas satur DNS secību saskaņā ar 4. punktu.
- 16. Vektors saskaņā ar 15. punktu, kurā DNS secība atrodas starp regulējošām secībām.
- 17. Vektors saskaņā ar 16. punktu, kurā regulējošais apgabals atrodas pretstraumes virzienā no ATG kodona un tam ir šāda nukleotīdu secība: AGATCTCCTCGTCCCACCGGCTGTCGAAGCTCCGCGCCTACAGCGCCAT CGACTTCGACCGGGAAATAGGAAGCGGCCGGCGACAAAACGGGGAGG GGCGGGAAACGTGGTCCGTTTCCCGCCCCTGCCCGTAGGCCGTGCGCG TCCCGCCGGTGCGCGGCGTTCAGCCCGCCGCCGCT.
- 18. Streptomyces saimniekšūna, kas transformēta ar vektoru saskaņā ar 15. punktu.
- 19. Paņēmiens polipeptīda iegūšanai, kas paredz saimniekšūnas saskaņā ar 18. punktu kultivēšanu.
- 20. Plazmīda pULAD3, kas satur saf gēnu. 2
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
LVP-95-230A LV11038B (en) | 1989-05-19 | 1995-07-26 | Saf polypeptide, gene |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/354,265 US5122595A (en) | 1989-05-19 | 1989-05-19 | SAF polypeptide gene coding therefor, and SAF promoter |
PCT/GB1990/000781 WO1990014426A2 (en) | 1989-05-19 | 1990-05-18 | Saf polypeptide, gene, promoter and use for expression in streptomyces |
LVP-93-184A LV10491B (en) | 1989-05-19 | 1993-03-12 | A cloning vehicle for expression of foreign genes in streptomyces cells |
LVP-95-230A LV11038B (en) | 1989-05-19 | 1995-07-26 | Saf polypeptide, gene |
Publications (2)
Publication Number | Publication Date |
---|---|
LV11038A LV11038A (lv) | 1996-02-20 |
LV11038B true LV11038B (en) | 1996-06-20 |
Family
ID=23392540
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
LVP-95-230A LV11038B (en) | 1989-05-19 | 1995-07-26 | Saf polypeptide, gene |
Country Status (5)
Country | Link |
---|---|
US (1) | US5122595A (lv) |
LT (1) | LT3312B (lv) |
LV (1) | LV11038B (lv) |
RU (1) | RU2114910C1 (lv) |
ZA (1) | ZA903833B (lv) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1993006219A1 (de) * | 1991-09-18 | 1993-04-01 | Hoechst Aktiengesellschaft | Sekundärmetabolit-biosynthesegene aus aktinomyceten, verfahren zu ihrer isolierung sowie ihre verwendung |
US5876987A (en) * | 1996-02-07 | 1999-03-02 | Board Of Trustees Operating Michigan State University | Method, DNA and bacteria for hyperproduction of an antibiotic due to disruption of an AbsA gene |
US6197591B1 (en) | 1998-09-14 | 2001-03-06 | Pfizer Inc. | Streptomyces avermitilis regulatory genes for increased avermectin production |
WO2012114260A1 (en) | 2011-02-21 | 2012-08-30 | Logomotion, S.R.O. | A mobile communication device for contactless payments, a payment method |
-
1989
- 1989-05-19 US US07/354,265 patent/US5122595A/en not_active Expired - Lifetime
-
1990
- 1990-05-18 ZA ZA903833A patent/ZA903833B/xx unknown
- 1990-05-18 RU SU4894397A patent/RU2114910C1/ru not_active IP Right Cessation
-
1993
- 1993-04-07 LT LTIP477A patent/LT3312B/lt not_active IP Right Cessation
-
1995
- 1995-07-26 LV LVP-95-230A patent/LV11038B/en unknown
Also Published As
Publication number | Publication date |
---|---|
ZA903833B (en) | 1991-05-29 |
LTIP477A (en) | 1994-10-25 |
RU2114910C1 (ru) | 1998-07-10 |
LV11038A (lv) | 1996-02-20 |
LT3312B (en) | 1995-06-26 |
US5122595A (en) | 1992-06-16 |
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