LV11038B - Saf polypeptide, gene - Google Patents

Saf polypeptide, gene Download PDF

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Publication number
LV11038B
LV11038B LVP-95-230A LV950230A LV11038B LV 11038 B LV11038 B LV 11038B LV 950230 A LV950230 A LV 950230A LV 11038 B LV11038 B LV 11038B
Authority
LV
Latvia
Prior art keywords
gene
ser
ala
saf
vector
Prior art date
Application number
LVP-95-230A
Other languages
English (en)
Other versions
LV11038A (lv
Inventor
Antonio Daza Ortega
Jose Antonio Gil
Tomas Vigal Garcia
Juan Francisco Martin
Original Assignee
Applied Research Systems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/GB1990/000781 external-priority patent/WO1990014426A2/en
Application filed by Applied Research Systems filed Critical Applied Research Systems
Priority to LVP-95-230A priority Critical patent/LV11038B/en
Publication of LV11038A publication Critical patent/LV11038A/lv
Publication of LV11038B publication Critical patent/LV11038B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (20)

  1. LV 11038 Izgudrojuma formula 1. Būtībā tīrs polipeptīds ar šādu aminoskābju secību: Met Cys Ser Ala Vai Vai Ala Ala Ala Vai Ser Ser Met Ser Arg Arg Arg Arg Arg Ala Ser Ala Thr Arg Arg Ser Ala Ala Vai Ser Pro Pro His Thr Pro Tyr Gly Ser Gly Cys lle Ser Ala Cys Ser Trp Hys Ser Thr lle Thr Gly His Arg Ala Gln Thr Arg Leu Ala Ala Ser Ser Arg Ala Ser Arg Ala Ala Vai Gly Ser Phe Asp Gly Ala Lys Asn Arg Pro Ala Ser Ser Arg Arg Gln Ala Ala Ser Gly Cys Gln Gly Pro Ala Ser Ser Ser Arg Ala Gly Vai Arg Trp Ala Gly Thr Ala Ala Asn Gly Arg Gly Ser un/vai tā fragmenti ar sekundārā metabolismu aktivējošā faktora aktivitāti.
  2. 2. DNS secība, kas kodē polipeptīdu saskaņā ar 1. punktu.
  3. 3. DNS secība, kurā ietverta šāda nukleotīdu secība: ATG TGC TCG GCC GTC GTC GCG GCG GCC GTG AGC TGC ATG TCC CGC CGT CGC CGC CGG GCC AGC GCC ACC CGG CGC TCC GCT GGG GTG AGC CCG CCC CAC ACT CCG TAC CGC TCG GGC TGT ACT AGC GCG TGC TCC TGG CAC TCC ACC ATC ACC GGA CAC CGG GCA CAG ACC CGC TTG GCC GCC TCC TCG CGG GCC AGC CGG GCG GCG GTC GGC TCC TTC GAC GGG GCG AAG AAC AGG CCG GCC TCG TCG CGG CGG CAG GCC GCA TCG GAA TCG CAG GGG CCC GCG TCG TCC TCC CGT GCG GGG GTC CGC TGG GCC GGA ACG GCG GCG AAC GGC AGA GGC TGA.
  4. 4. DNS secība, kas hibridizējas ar nukleotīdu secību saskaņā ar 3. punktu, un kas kodē SAF tipa polipeptīdu.
  5. 5. Vektors, kas ietver DNS secību saskaņā ar 2. punktu.
  6. 6. Vektors saskaņā ar 5. punktu, kurā DNS secība atrodas starp regulējošām secībām.
  7. 7. Vektors saskaņā ar 6. punktu, kurā regulējošais apgabals atrodas pretstraumes virzienā no ATG kodona un tam ir šāda nukleotīdu secība: 1 AGATCTCCTCGTCCCACCGGCTGTCGAAGCTCCGCGCCTACAGCGCCAT CGACTTCGACCGGGAAATAGGAAGCGGCCGGCGACAAAACGGGGAGG GGCGGGAAACGTGGTCCGTTTCCCGCCCCTGCCCGTAGGCCGTGCGCG TCCCGCCGGTGCGCGGCGTTCAGCCCGCCGCCGCT.
  8. 8. Streptomyces saimniekšūna, kas transformēta ar vektoru saskaņā ar 5. punktu.
  9. 9. Paņēmiens polipeptīda iegūšanai, kas paredz kultivēt saimniekšūnu saskaņā ar 8. punktu.
  10. 10. Vektors, kas satur DNS secību saskaņā ar 3. punktu.
  11. 11. Vektors saskaņā ar 10. punktu, kurā DNS secība atrodas starp regulējošām secībām.
  12. 12. Vektors saskaņā ar 11. punktu, kurā regulējošais apgabals atrodas pretstraumes virzienā no ATG kodona un tam ir šāda nukleotīdu secība: AGATCTCCTCGTCCCACCGGCTGTCGAAGCTCCGCGCCTACAGCGCCAT CGACTTCGACCGGGAAATAGGAAGCGGCCGGCGACAAAACGGGGAGG GGCGGGAAACGTGGTCCGTTTCCCGCCCCTGCCCGTAGGCCGTGCGCG TCCCGCCGGTGCGCGGCGTTCAGCCCGCCGCCGCT.
  13. 13. Streptomyces saimniekšūna, kas transformēta ar vektoru saskaņā ar 10. punktu.
  14. 14. Paņēmiens polipeptīda iegūšanai, kas paredz saimniekšūnas saskaņā ar 13. punktu kultivēšanu.
  15. 15. Vektors, kas satur DNS secību saskaņā ar 4. punktu.
  16. 16. Vektors saskaņā ar 15. punktu, kurā DNS secība atrodas starp regulējošām secībām.
  17. 17. Vektors saskaņā ar 16. punktu, kurā regulējošais apgabals atrodas pretstraumes virzienā no ATG kodona un tam ir šāda nukleotīdu secība: AGATCTCCTCGTCCCACCGGCTGTCGAAGCTCCGCGCCTACAGCGCCAT CGACTTCGACCGGGAAATAGGAAGCGGCCGGCGACAAAACGGGGAGG GGCGGGAAACGTGGTCCGTTTCCCGCCCCTGCCCGTAGGCCGTGCGCG TCCCGCCGGTGCGCGGCGTTCAGCCCGCCGCCGCT.
  18. 18. Streptomyces saimniekšūna, kas transformēta ar vektoru saskaņā ar 15. punktu.
  19. 19. Paņēmiens polipeptīda iegūšanai, kas paredz saimniekšūnas saskaņā ar 18. punktu kultivēšanu.
  20. 20. Plazmīda pULAD3, kas satur saf gēnu. 2
LVP-95-230A 1989-05-19 1995-07-26 Saf polypeptide, gene LV11038B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
LVP-95-230A LV11038B (en) 1989-05-19 1995-07-26 Saf polypeptide, gene

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US07/354,265 US5122595A (en) 1989-05-19 1989-05-19 SAF polypeptide gene coding therefor, and SAF promoter
PCT/GB1990/000781 WO1990014426A2 (en) 1989-05-19 1990-05-18 Saf polypeptide, gene, promoter and use for expression in streptomyces
LVP-93-184A LV10491B (en) 1989-05-19 1993-03-12 A cloning vehicle for expression of foreign genes in streptomyces cells
LVP-95-230A LV11038B (en) 1989-05-19 1995-07-26 Saf polypeptide, gene

Publications (2)

Publication Number Publication Date
LV11038A LV11038A (lv) 1996-02-20
LV11038B true LV11038B (en) 1996-06-20

Family

ID=23392540

Family Applications (1)

Application Number Title Priority Date Filing Date
LVP-95-230A LV11038B (en) 1989-05-19 1995-07-26 Saf polypeptide, gene

Country Status (5)

Country Link
US (1) US5122595A (lv)
LT (1) LT3312B (lv)
LV (1) LV11038B (lv)
RU (1) RU2114910C1 (lv)
ZA (1) ZA903833B (lv)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993006219A1 (de) * 1991-09-18 1993-04-01 Hoechst Aktiengesellschaft Sekundärmetabolit-biosynthesegene aus aktinomyceten, verfahren zu ihrer isolierung sowie ihre verwendung
US5876987A (en) * 1996-02-07 1999-03-02 Board Of Trustees Operating Michigan State University Method, DNA and bacteria for hyperproduction of an antibiotic due to disruption of an AbsA gene
US6197591B1 (en) 1998-09-14 2001-03-06 Pfizer Inc. Streptomyces avermitilis regulatory genes for increased avermectin production
WO2012114260A1 (en) 2011-02-21 2012-08-30 Logomotion, S.R.O. A mobile communication device for contactless payments, a payment method

Also Published As

Publication number Publication date
ZA903833B (en) 1991-05-29
LTIP477A (en) 1994-10-25
RU2114910C1 (ru) 1998-07-10
LV11038A (lv) 1996-02-20
LT3312B (en) 1995-06-26
US5122595A (en) 1992-06-16

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