LT5317B - Cow fodder for improving quality of milk - Google Patents
Cow fodder for improving quality of milk Download PDFInfo
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- LT5317B LT5317B LT2004041A LT2004041A LT5317B LT 5317 B LT5317 B LT 5317B LT 2004041 A LT2004041 A LT 2004041A LT 2004041 A LT2004041 A LT 2004041A LT 5317 B LT5317 B LT 5317B
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- milk
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- lysozyme
- cows
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- 235000013336 milk Nutrition 0.000 title abstract description 30
- 239000008267 milk Substances 0.000 title abstract description 30
- 210000004080 milk Anatomy 0.000 title abstract description 30
- 241000283690 Bos taurus Species 0.000 claims abstract description 40
- 241001465754 Metazoa Species 0.000 claims abstract description 24
- 239000000203 mixture Substances 0.000 claims abstract description 21
- 210000001082 somatic cell Anatomy 0.000 claims abstract description 7
- 239000000654 additive Substances 0.000 claims abstract 5
- 230000000996 additive effect Effects 0.000 claims abstract 5
- 108010014251 Muramidase Proteins 0.000 claims description 22
- 102000016943 Muramidase Human genes 0.000 claims description 22
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 22
- 229960000274 lysozyme Drugs 0.000 claims description 22
- 239000004325 lysozyme Substances 0.000 claims description 22
- 235000010335 lysozyme Nutrition 0.000 claims description 22
- 108010062670 lysosubtilin G10X Proteins 0.000 claims description 16
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 229940088598 enzyme Drugs 0.000 claims description 13
- 230000002934 lysing effect Effects 0.000 claims description 8
- 235000020247 cow milk Nutrition 0.000 claims description 5
- 229940079919 digestives enzyme preparation Drugs 0.000 claims description 5
- 230000002255 enzymatic effect Effects 0.000 claims description 3
- 239000011782 vitamin Substances 0.000 claims description 3
- 235000013343 vitamin Nutrition 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 238000009472 formulation Methods 0.000 claims 1
- 239000013589 supplement Substances 0.000 claims 1
- 239000011573 trace mineral Substances 0.000 claims 1
- 235000013619 trace mineral Nutrition 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 10
- 230000037396 body weight Effects 0.000 abstract description 7
- 235000013365 dairy product Nutrition 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 10
- 239000003674 animal food additive Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 210000000987 immune system Anatomy 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 239000004463 hay Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000008092 positive effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000004552 water soluble powder Substances 0.000 description 2
- 108010011619 6-Phytase Proteins 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 229920002498 Beta-glucan Polymers 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 108010001682 Dextranase Proteins 0.000 description 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241001454523 Quillaja saponaria Species 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000003625 amylolytic effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 235000013861 fat-free Nutrition 0.000 description 1
- 238000003872 feeding technique Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 229960001438 immunostimulant agent Drugs 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 208000004396 mastitis Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000004460 silage Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019195 vitamin supplement Nutrition 0.000 description 1
Landscapes
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
Description
Išradimas yra susijęs su karvių pašaro kompozicija, gerinančia pieno kokybę sumažinant somatinių ląstelių kiekį piene, taip pat su lizocimo ir kitų lizuojančių fermentų preparatų, tokių kaip lizocimas G3x ir lizosubtilinas G10x panaudojimu pašarų kompozicijos gamyboje.The present invention relates to a cow's feed composition which improves milk quality by reducing the amount of somatic cells in milk, and to the use of lysozyme and other lysing enzyme preparations such as lysozyme G3x and lysosubtilin G10x in the manufacture of a feed composition.
Pieno kokybė ir sudėtis priklauso nuo daugelio faktorių: pašarų kokybės ir šėrimo technikos, melžimo, karvių veislės, gyvulių fiziologinės būklės ir kt. Somatinių ląstelių kiekis (SLK) yra vienas iš pagrindinių karvių pieno kokybės rodiklių. Somatinių ląstelių piene yra visada. Kai SLK viršija 300.000 ląstelių/mį pieno kokybė mažėja. SLK, didesnis nei 300.000 ląstelių/ml, nurodo, kad karvės tešmuo yra sudirgintas arba nesveikas. Pagal 2000 m. reikalavimus, taikomus Lietuvoje, aukščiausios rūšies piene SLK negali būti didesnis nei 400.000 ląstelių/ml, pirmos rūšies piene - iki 500.000 ląstelių/ml, o antros rūšies piene - iki 700.000 ląstelių/ml. Pagal ES pastarųjų metų reikalavimus SLK piene, skirtam žmonių maistui, negali būti didesnis nei 300.000 ląstelių/ml.The quality and composition of milk depends on many factors: feed quality and feeding technique, milking, cow breed, physiological status of animals, etc. Somatic cell count (SLK) is one of the main indicators of cow's milk quality. Somatic cells are always present in milk. When the SLK exceeds 300,000 cells / m, the quality of milk decreases. SLK greater than 300,000 cells / ml indicates that the cow's udder is irritated or unhealthy. According to 2000 In Lithuania, SLK must not exceed 400,000 cells / ml in the highest quality milk, up to 500,000 cells / ml in the first grade milk and 700,000 cells / ml in the second grade milk. SLK milk for human consumption must not exceed 300,000 cells / ml according to EU requirements in recent years.
Žvelgiant iš kito taško, SLK yra karvių imuninės būklės rodiklis. Kai SLK piene viršija 300.000 ląstelių/ml, galima teigti, kad karvių imuninė sistema nesugeba pasipriešinti išorės poveikiams/infekcijai. Todėl karvių organizmo rezistentiškumo aplinkos faktoriams padidinimas yra kartu ir vienas iš galimų SLK piene sumažinimo bei pieno kokybės pagerinimo būdų. Tam gali būti naudojamos įvairios medžiagos, kurios gyvuliui skiriamos kartu su pašaru. Tai galėtų būti mikroelementai ir/arba vitaminai, kurių teigiamas poveikis imuninei sistemai yra neabejotinas, tačiau galima vartoti ir kitas medžiagas. Taip, pavyzdžiui, SLK piene sumažinti vartojami pašarų priedai, kuriuose veiklioji medžiaga yra kininio arbatmedžio milteliai ir/arba ekstraktas (JP 7 075 506); augalo Quillaja saponaria ekstrakto milteliai (EP 1 219 178). Šių būdų efektyvumas nėra didelis.From another perspective, SLK is an indicator of the immune status of cows. When the SLK in milk exceeds 300,000 cells / ml, it can be argued that the cow's immune system is unable to withstand external influences / infection. Therefore, increasing cow's resistance to environmental factors is also one of the possible ways to reduce SLK in milk and improve milk quality. For this purpose, various materials may be used which are administered to the animal in the same feed. These could be micronutrients and / or vitamins, which have a clear positive effect on the immune system, but other substances can be used. For example, feed additives containing Chinese tea powder and / or extract as the active ingredient are used to reduce SLK milk (JP 7 075 506); powder of extract of plant Quillaja saponaria (EP 1 219 178). The effectiveness of these techniques is not great.
Mes gi siūlome kaip pašarų priedus naudoti fermentinius preparatus. Žinoma, kad fermentai, kaip pašarų priedai, naudojami: pašaro konservavimui (karbohidratus skaidantys fermentai ) (EP 0 346 909); pieno sekrecijos bei neriebalinės sausų medžiagų dalies padidinimui (celiuliazė, ksilanazė, dekstranazė, amilazė, pektinazė, proteazė) (US 4 144 354). Patentinėje publikacijoje EP 1 043 028 A1 aprašytas fitazės panaudojimas mastito, kaip somatinių ląstelių piene priežasties, gydymui.We suggest using enzyme preparations as feed additives. Enzymes are known to be used as feed additives for: feed preservation (carbohydrate-degrading enzymes) (EP 0 346 909); to increase milk secretion and non-fat solids (cellulase, xylanase, dextranase, amylase, pectinase, protease) (US 4,144,354). EP 1 043 028 A1 describes the use of phytase for the treatment of mastitis as a cause of somatic cells in milk.
Tačiau nė vienas iš čia paminėtų fermentų neturi ryškaus poveikio gyvulių imuninei sistemai.However, none of the enzymes mentioned herein has a significant effect on the immune system of animals.
Siūlome naudoti tokius fermentus, kurie įgalintų ženkliai padidinti karvių organizmo rezistentiškumą o ir tuo pačiu turėtų ryškų poveikį SLK piene sumažinti ir pieno kokybei pagerinti. Tai galėtų būti lizuojantys fermentai, tokie kaip lizocimas, kurie, ardydami virškinimo trakte esančių mikrobinių ląstelių sieneles, atpalaiduoja imuninę sistemą stimuliuojančias medžiagas - muropeptidus, lipopolisacharidus, β-gliukanus ir kt.We suggest the use of enzymes that can significantly increase the resistance of cows and at the same time have a significant effect on reducing SLK in milk and improving milk quality. These could be lysing enzymes, such as lysozyme, which release immune stimulants such as muropeptides, lipopolysaccharides, β-glucans, and the like by breaking down the microbial cell walls of the gastrointestinal tract.
Šieme išradime siūloma lizuojančių fermentų šaltiniu naudoti preparatus lizocimas G3x ir lizosubtilinas G10x. Tai yra baltyminiai preparatai, todėl jie po įvedimo per os ir savo funkcijos įvykdymo, yra suvirškinami kaip ir kiti baltymai. Jie yra netoksiški, nesikaupia organizme, neturi šalutinio poveikio, jų poveikyje nesusidaro rezistenciškos antimikrobiniams veiksniams mikroorganizmų formos.The present invention provides for the use of lysozyme G3x and lysosubtilin G10x as a source of lysing enzymes. These are proteinaceous preparations and are therefore digested like other proteins after oral administration and fulfillment of their function. They are non-toxic, do not accumulate in the body, have no side effects, and do not form micro-organisms resistant to antimicrobial agents.
Lizocimas G3x yra fermentinis preparatas, gaunamas išdžiovinus purkštuvinėje džiovykloje išaugintų giluminiu būdu bakterijų Bacillus subtilis kultūrinį skystį. Tai yra šviesiai pilkos spalvos gerai tirpstantys vandenyje milteliai. Preparato lizociminis aktyvumas yra 300±30 vnt/g.Lysozyme G3x is an enzyme preparation obtained by drying the culture fluid of deep-grown Bacillus subtilis bacteria in a spray dryer. It is a light gray, water-soluble powder. The lysozyme activity of the preparation is 300 ± 30 units / g.
Lizosubtilinas G10x yra kompleksinis fermentinis preparatas, gaunamas nusodinus giluminiu būdu išaugintų bakterijų Bacillus subtilis kultūrinio skysčio filtrate esantį baltymą acetonu. Tai yra smulkūs, šviesiai pilkos arba smėlio spalvos tirpūs vandenyje milteliai. Preparato lizuojantis aktyvumas yra ne mažesnis nei 1000000 vnt/g. Preparato sudėtyje yra ir amilolizinių bei proteolizinių fermentų.Lysosubtilin G10x is a complex enzyme preparation obtained by precipitation of acetone in the culture fluid filtrate of deep-grown bacteria Bacillus subtilis. It is a fine, light gray or beige water soluble powder. The lysing activity of the preparation shall be not less than 1000000 units / g. It also contains amylolytic and proteolytic enzymes.
Pagal šį išradimą į įprastinius karvių pašarus įmaišoma 100-500 mg/kg gyvulio masės, geriausia, 200 mg/kg gyvulio masės lizocimo G3x, ir sušeriama kartą dienoje. Karvės šeriamos tokiu mišiniu bent 5-30 dienų.According to the present invention 100 to 500 mg / kg of animal weight, preferably 200 mg / kg of lysozyme G3x, is added to conventional cow feed and is administered once daily. Cows are fed this mixture for at least 5-30 days.
Pagal kitą išradimo atvejį į įprastinius karvių pašarus įmaišoma 10-50 mg/kg gyvulio masės, geriausia, 20 mg/kg gyvulio masės lizosubtilino G10x, ir sušeriama kartą dienoje. Karvės šeriamos tokiu mišiniu bent 5-30 dienų.According to another embodiment of the invention, 10 to 50 mg / kg animal weight, preferably 20 mg / kg lysosubtilin G10x, is added to conventional cows feed and is administered once daily. Cows are fed this mixture for at least 5-30 days.
Pagal dar vieną išradimo atvejį į įprastinius karvių pašarus įmaišoma 200 mg/kg gyvulio masės lizocimo G3x ir 20 mg/kg gyvulio masės lizosubtilino G10x ir sušeriama kartą dienoje. Karvės šeriamos tokiu mišiniu bent 5-30 dienų.In another embodiment of the invention, 200 mg / kg lysozyme G3x and 20 mg / kg lysosubtilin G10x are added to conventional cow feeds and are administered once daily. Cows are fed this mixture for at least 5-30 days.
įprastinis karvių pašaras yra toks, kuris gerai žinomas šios srities specialistui. Tai gali būti racionas, susidedantis iš šienainio, siloso, šieno, šiaudų, saladino, cukrinių runkelių, kombinuotųjų pašarų, mineralinių vitamininių priedų, laižomosios druskos. Ganykliniu laikotarpiu karvių racioną gali sudaryti ganyklų žolė, saladinas, kombinuotasis pašaras, laižomoji druska.conventional cow feed is one that is well known to those skilled in the art. It can be a diet consisting of hay, silage, hay, straw, saladine, sugar beet, compound feed, mineral vitamin supplements, licking salt. During the grazing period, the cow's diet can consist of pasture grass, saladin, compound feed, licking salt.
Pieno tyrimai atliekami žinomais šioje srityje metodais. SLK nustatomas kiekvienai karvei individualiai, naudojant, pavyzdžiui, prietaisą “Somascope”. Pašarų priedo pagal šį išradimą teigiamas efektas, sumažinant SLK piene, įvertinamas SLK po bandymo, lyginant su pradiniu kiekiu, procentais.Dairy tests are carried out using methods known in the art. The SLK is set individually for each cow using, for example, a Somascope instrument. The positive effect of a feed additive according to the present invention in reducing SLK in milk is evaluated as a percentage of the post-test SLK relative to the starting amount.
Siūlomos pašarų kompozicijos naudojimas bent 10 dienų sumažina SLK karvių piene daugiau nei tris kartus ir tuo pačiu reikšmingai pagerina pieno kokybę.Using the proposed feed composition for at least 10 days reduces SLK in cow's milk by more than three times, and at the same time significantly improves milk quality.
Toliau išradimas iliustruojamas pavyzdžiais.The invention is further illustrated by the following examples.
Pavyzdys 1.Example 1.
Bandymui panaudotos keturios Lietuvos juodmargių karvių grupės, po penkias karves grupėje. Karvės laikomos, šeriamos ir melžiamos kaip įprasta, išskyrus tai, kad trijų grupių karvėms dešimties dienų laikotarpyje papildomai duodamas lizosubtilinas G10x kartu su pašaru kartą dienoje. Kontrolinė karvių grupė lizosubtilino G10x negauna; pirma bandomoji grupė gauna lizosubtilino G10x 5 mg/kg gyvulio masės; antra bandomoji grupė gauna lizosubtilino G10x 10 mg/kg gyvulio masės; trečia bandomoji grupė gauna lizosubtilino G10x 20 mg/kg gyvulio masės. SLK karvių piene nustatomas prieš bandymą ir jį baigus. Rezultatai pateikti lentelėje.Four groups of Lithuanian Black and White cows were used for the test, five cows per group. Cows are kept, fed and milked as usual, except that three cows are given lysosubtilin G10x for a period of ten days in addition to feed once a day. A control group of cows does not receive lysosubtilin G10x; the first treatment group receives lysosubtilin G10x 5 mg / kg animal weight; the second treatment group received lysosubtilin G10x 10 mg / kg animal weight; the third treatment group receives lysosubtilin G10x 20 mg / kg animal weight. The SCF in cow's milk is determined before and at the end of the test. The results are shown in the table.
Pavyzdys 2.Example 2.
Bandymui panaudotos keturios Lietuvos juodmargių karvių grupės, po penkias karves grupėje. Karvės laikomos, šeriamos ir melžiamos kaip įprasta, išskyrus tai, kad trijų grupių karvėms dešimties dienų laikotarpyje papildomai duodamas lizocimas G3x kartu su pašaru kartą dienoje. Kontrolinė karvių grupė lizocimo G3x negauna; pirma bandomoji grupė gauna lizocimo G3x 50 mg/kg gyvulio masės; antra bandomoji grupė gauna lizocimo G3x 100 mg/kg gyvulio masės; trečia bandomoji grupė gauna lizocimo G3x 200 mg/kg gyvulio masės. SLK karvių piene nustatomas prieš bandymą ir jį baigus. Rezultatai pateikti lentelėje.Four groups of Lithuanian Black and White cows were used for the test, five cows per group. Cows are kept, fed and milked as usual, except that three groups of cows are given lysozyme G3x together with feed once daily for a period of ten days. The control group of cows does not receive lysozyme G3x; the first treatment group received lysozyme G3x 50 mg / kg animal weight; the second treatment group received lysozyme G3x 100 mg / kg animal weight; the third treatment group receives lysozyme G3x 200 mg / kg animal weight. The SCF in cow's milk is determined before and at the end of the test. The results are shown in the table.
Pavyzdys 3.Example 3.
Bandymui panaudotos trys Lietuvos juodmargių karvių grupės, po devynias karves grupėje. Karvės laikomos, šeriamos ir melžiamos kaip įprasta, išskyrus tai, kad dviejų grupių karvėms penkiolikos dienų laikotarpyje papildomai duodami fermentiniai preparatai (su vitaminais arba be jų) kartu su pašaru kartą dienoje. Kontrolinė karvių grupė fermentų negauna; pirma bandomoji grupė gauna lizosubtilino G10x 20 mg/kg gyvulio masės + lizocimo G3x 200 mg/kg gyvulio masės ; antra bandomoji grupė gauna lizocimo G3xThree groups of Lithuanian Black and White cows were used for the test, nine cows per group. Cows are kept, fed and milked as usual, except that two groups of cows receive supplemental enzymatic preparations (with or without vitamins) for a period of fifteen days in addition to feed once daily. A control group of cows does not receive enzymes; the first treatment group received lysosubtilin G10x 20 mg / kg animal weight + lysozyme G3x 200 mg / kg animal weight; the second test group receives lysozyme G3x
200 mg/kg gyvulio masės + vitaminų C, A, E. SLK karvių piene nustatomas prieš bandymą ir jį baigus. Rezultatai pateikti lentelėje.200 mg / kg body weight + vitamins C, A, E. SLK in cows' milk is determined before and after the test. The results are shown in the table.
Claims (9)
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4144354A (en) | 1976-02-12 | 1979-03-13 | Kyowa Hakko Kogyo Co., Ltd. | Method for promoting secretion of milk of livestock |
JPH0775506A (en) | 1993-09-09 | 1995-03-20 | Suntory Ltd | Milk quality improver and feed formulated therewith |
EP1043028A1 (en) | 1997-12-19 | 2000-10-11 | Kyowa Hakko Kogyo Co., Ltd. | Method for preventing/remedying mastitis |
EP1219178A1 (en) | 2000-12-27 | 2002-07-03 | Nor-Feed ApS | Oral composition for ensuring a high milk quality |
-
2004
- 2004-04-29 LT LT2004041A patent/LT5317B/en not_active IP Right Cessation
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4144354A (en) | 1976-02-12 | 1979-03-13 | Kyowa Hakko Kogyo Co., Ltd. | Method for promoting secretion of milk of livestock |
JPH0775506A (en) | 1993-09-09 | 1995-03-20 | Suntory Ltd | Milk quality improver and feed formulated therewith |
EP1043028A1 (en) | 1997-12-19 | 2000-10-11 | Kyowa Hakko Kogyo Co., Ltd. | Method for preventing/remedying mastitis |
EP1219178A1 (en) | 2000-12-27 | 2002-07-03 | Nor-Feed ApS | Oral composition for ensuring a high milk quality |
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