LT3040424T - Modifikuoti baltymai ir jų gavimo ir panaudojimo būdai - Google Patents
Modifikuoti baltymai ir jų gavimo ir panaudojimo būdaiInfo
- Publication number
- LT3040424T LT3040424T LTEP15192848.8T LT15192848T LT3040424T LT 3040424 T LT3040424 T LT 3040424T LT 15192848 T LT15192848 T LT 15192848T LT 3040424 T LT3040424 T LT 3040424T
- Authority
- LT
- Lithuania
- Prior art keywords
- making
- methods
- same
- modified proteins
- proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US30886310P | 2010-02-26 | 2010-02-26 | |
PCT/US2011/026228 WO2011106634A2 (en) | 2010-02-26 | 2011-02-25 | Modified proteins and methods of making and using same |
Publications (1)
Publication Number | Publication Date |
---|---|
LT3040424T true LT3040424T (lt) | 2018-05-10 |
Family
ID=44507583
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
LTEP15192848.8T LT3040424T (lt) | 2010-02-26 | 2011-02-28 | Modifikuoti baltymai ir jų gavimo ir panaudojimo būdai |
Country Status (6)
Country | Link |
---|---|
US (2) | US20110262903A1 (lt) |
EP (4) | EP2539471B1 (lt) |
CN (1) | CN103097547B (lt) |
AU (1) | AU2011220536B2 (lt) |
LT (1) | LT3040424T (lt) |
WO (4) | WO2011106629A2 (lt) |
Families Citing this family (61)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
CA2672315A1 (en) | 2006-12-14 | 2008-06-26 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes using large scale fet arrays |
US8262900B2 (en) * | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
US11339430B2 (en) | 2007-07-10 | 2022-05-24 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
WO2010008480A2 (en) | 2008-06-25 | 2010-01-21 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes using large scale fet arrays |
US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
US20120261274A1 (en) | 2009-05-29 | 2012-10-18 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
US8776573B2 (en) | 2009-05-29 | 2014-07-15 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
US20120202276A1 (en) | 2010-02-26 | 2012-08-09 | Life Technologies Corporation | Modified Proteins and Methods of Making and Using Same |
EP2539471B1 (en) * | 2010-02-26 | 2014-08-06 | Life Technologies Corporation | Method for sequencing using a modified polymerase |
CN106932456B (zh) | 2010-06-30 | 2020-02-21 | 生命科技公司 | 用于测试isfet阵列的方法和装置 |
JP2013533482A (ja) | 2010-06-30 | 2013-08-22 | ライフ テクノロジーズ コーポレーション | イオン感応性電荷蓄積回路および方法 |
CN106449632B (zh) | 2010-06-30 | 2019-09-20 | 生命科技公司 | 阵列列积分器 |
US11307166B2 (en) | 2010-07-01 | 2022-04-19 | Life Technologies Corporation | Column ADC |
JP5876044B2 (ja) | 2010-07-03 | 2016-03-02 | ライフ テクノロジーズ コーポレーション | 低濃度ドープドレインを有する化学的感応性センサ |
EP2617061B1 (en) | 2010-09-15 | 2021-06-30 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
WO2012039812A1 (en) | 2010-09-24 | 2012-03-29 | Life Technologies Corporation | Matched pair transistor circuits |
JP5907990B2 (ja) | 2010-12-17 | 2016-05-11 | ライフ テクノロジーズ コーポレーション | 核酸増幅のための方法、組成物、システム、装置およびキット |
US11208636B2 (en) | 2011-08-10 | 2021-12-28 | Life Technologies Corporation | Polymerase compositions, methods of making and using same |
CN103857805B (zh) * | 2011-08-10 | 2017-07-18 | 生命技术公司 | 聚合酶组合物、制备和使用所述聚合酶组合物的方法 |
US9970984B2 (en) | 2011-12-01 | 2018-05-15 | Life Technologies Corporation | Method and apparatus for identifying defects in a chemical sensor array |
US10030262B2 (en) | 2012-04-19 | 2018-07-24 | Life Technologies Corporation | Method of performing digital PCR |
EP3461910B1 (en) | 2012-04-19 | 2020-08-26 | Life Technologies Corporation | Nucleic acid amplification |
CN114854832A (zh) | 2012-04-19 | 2022-08-05 | 生命技术公司 | 核酸扩增 |
US8786331B2 (en) | 2012-05-29 | 2014-07-22 | Life Technologies Corporation | System for reducing noise in a chemical sensor array |
US9034606B2 (en) | 2012-08-23 | 2015-05-19 | New England Biolabs, Inc. | Detection of an amplification reaction product using pH-sensitive dyes |
EP2895620B1 (en) | 2012-09-11 | 2017-08-02 | Life Technologies Corporation | Nucleic acid amplification |
WO2014043143A1 (en) | 2012-09-11 | 2014-03-20 | Life Technologies Corporation | Nucleic acid amplification |
US9080968B2 (en) | 2013-01-04 | 2015-07-14 | Life Technologies Corporation | Methods and systems for point of use removal of sacrificial material |
US9841398B2 (en) | 2013-01-08 | 2017-12-12 | Life Technologies Corporation | Methods for manufacturing well structures for low-noise chemical sensors |
US8963216B2 (en) | 2013-03-13 | 2015-02-24 | Life Technologies Corporation | Chemical sensor with sidewall spacer sensor surface |
US9835585B2 (en) | 2013-03-15 | 2017-12-05 | Life Technologies Corporation | Chemical sensor with protruded sensor surface |
US9116117B2 (en) | 2013-03-15 | 2015-08-25 | Life Technologies Corporation | Chemical sensor with sidewall sensor surface |
JP6581074B2 (ja) | 2013-03-15 | 2019-09-25 | ライフ テクノロジーズ コーポレーション | 一貫性のあるセンサ表面積を有する化学センサ |
EP2972281B1 (en) | 2013-03-15 | 2023-07-26 | Life Technologies Corporation | Chemical device with thin conductive element |
EP2972280B1 (en) | 2013-03-15 | 2021-09-29 | Life Technologies Corporation | Chemical sensor with consistent sensor surface areas |
US20140336063A1 (en) | 2013-05-09 | 2014-11-13 | Life Technologies Corporation | Windowed Sequencing |
US10458942B2 (en) | 2013-06-10 | 2019-10-29 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
WO2015048763A1 (en) * | 2013-09-30 | 2015-04-02 | Life Technologies Corporation | Polymerase compositions, methods of making and using same |
US10758700B2 (en) | 2013-09-30 | 2020-09-01 | Arizona Board Of Regents On Behalf Of The University Of Arizona | Home-based heliox system with carbon dioxide removal |
CN104212711B (zh) * | 2014-04-08 | 2017-10-27 | 上海小海龟科技有限公司 | 电子传感器及基于电子传感器的基因探测方法 |
WO2016077324A1 (en) | 2014-11-11 | 2016-05-19 | Life Technologies Corporation | Thiolated nucleotide analogues for nucleic acid synthesis |
CN107208074B (zh) * | 2014-12-16 | 2021-06-15 | 生命技术公司 | 聚合酶组合物和制造与使用其的方法 |
CN111505087A (zh) | 2014-12-18 | 2020-08-07 | 生命科技公司 | 使用大规模 fet 阵列测量分析物的方法和装置 |
US10077472B2 (en) | 2014-12-18 | 2018-09-18 | Life Technologies Corporation | High data rate integrated circuit with power management |
EP3234576B1 (en) | 2014-12-18 | 2023-11-22 | Life Technologies Corporation | High data rate integrated circuit with transmitter configuration |
WO2017058810A2 (en) * | 2015-10-01 | 2017-04-06 | Life Technologies Corporation | Polymerase compositions and kits, and methods of using and making the same |
WO2017196676A1 (en) | 2016-05-10 | 2017-11-16 | Life Technologies Corporation | Metal chelation post incorporation detection methods |
US11268117B2 (en) | 2016-06-10 | 2022-03-08 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
WO2018054970A2 (en) * | 2016-09-22 | 2018-03-29 | F. Hoffmann-La Roche Ag | Pol6 polymerase variants |
CN110573616B (zh) * | 2017-04-18 | 2023-09-05 | 深圳华大智造科技股份有限公司 | Phi29 DNA聚合酶及其编码基因与应用 |
US11163726B2 (en) | 2017-08-31 | 2021-11-02 | International Business Machines Corporation | Context aware delta algorithm for genomic files |
WO2019094524A1 (en) | 2017-11-07 | 2019-05-16 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
WO2019226689A1 (en) | 2018-05-22 | 2019-11-28 | Axbio Inc. | Methods, systems, and compositions for nucleic acid sequencing |
CN112823392A (zh) * | 2018-10-12 | 2021-05-18 | 生命科技股份有限公司 | 用于评估微卫星不稳定性状态的方法和*** |
EP3963103B1 (en) | 2019-05-03 | 2024-02-28 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
CN111073871B (zh) * | 2019-12-17 | 2021-02-26 | 中国科学院苏州生物医学工程技术研究所 | 热稳定性提高的dna聚合酶突变体及其构建方法和应用 |
CN113583996B (zh) * | 2021-09-29 | 2022-01-14 | 翌圣生物科技(上海)股份有限公司 | Bst DNA聚合酶重组突变体、其编码DNA及超快磁珠LAMP检测方法 |
Family Cites Families (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5466591A (en) | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
US5198543A (en) * | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
US5001050A (en) | 1989-03-24 | 1991-03-19 | Consejo Superior Investigaciones Cientificas | PHφ29 DNA polymerase |
US5508192A (en) | 1990-11-09 | 1996-04-16 | Board Of Regents, The University Of Texas System | Bacterial host strains for producing proteolytically sensitive polypeptides |
US5264365A (en) | 1990-11-09 | 1993-11-23 | Board Of Regents, The University Of Texas System | Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides |
US5767063A (en) | 1991-03-29 | 1998-06-16 | Genentech, Inc. | Human IL-8 receptor and antibodies thereto |
WO1993006217A1 (en) | 1991-09-19 | 1993-04-01 | Genentech, Inc. | EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES |
WO1994016107A1 (en) * | 1993-01-12 | 1994-07-21 | Thomas Jefferson University | DNA SEQUENCING WITH Bst POLYMERASE |
US5427928A (en) | 1993-03-24 | 1995-06-27 | Slesarev; Alexei I. | Thermostable DNA Topoisomerase V |
US5639635A (en) | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
US6184344B1 (en) | 1995-05-04 | 2001-02-06 | The Scripps Research Institute | Synthesis of proteins by native chemical ligation |
US6165765A (en) * | 1995-10-18 | 2000-12-26 | Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences | DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides |
US6027888A (en) | 1996-04-05 | 2000-02-22 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells |
SG86311A1 (en) * | 1997-04-10 | 2002-02-19 | Univ Singapore | Bacillus stearothermophilus dna polymerase i (klenow) clones with reduced 3'-to-5' exonuclease activity |
US6083715A (en) | 1997-06-09 | 2000-07-04 | Board Of Regents, The University Of Texas System | Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells |
US20030138834A1 (en) * | 1998-08-19 | 2003-07-24 | Dawson Elliott P. | Method for determining polynucleotide sequence variations |
WO2001023411A2 (en) * | 1999-09-30 | 2001-04-05 | New England Biolabs, Inc. | Incorporation of modified nucleotides by archaeon dna polymerases and related methods |
US6627424B1 (en) | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
US20030134292A1 (en) * | 2001-10-30 | 2003-07-17 | Farchaus Joseph W. | Thermostable DNA polymerases and methods of making same |
JP4632788B2 (ja) | 2002-09-20 | 2011-02-16 | ニュー・イングランド・バイオラブズ・インコーポレイティッド | 核酸のヘリカーゼ依存性増幅 |
DE602004024034D1 (de) | 2003-01-29 | 2009-12-24 | 454 Corp | Nukleinsäureamplifikation auf basis von kügelchenemulsion |
GB0321306D0 (en) * | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
GB0509508D0 (en) * | 2005-05-10 | 2005-06-15 | Solexa Ltd | Improved polymerases |
US20070059713A1 (en) | 2005-09-09 | 2007-03-15 | Lee Jun E | SSB-DNA polymerase fusion proteins |
US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
CA2633524A1 (en) * | 2005-12-22 | 2007-07-05 | Pacific Biosciences Of California, Inc. | Polymerases for nucleotide analogue incorporation |
US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
US8262900B2 (en) * | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
CA2672315A1 (en) * | 2006-12-14 | 2008-06-26 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes using large scale fet arrays |
US7972828B2 (en) * | 2006-12-19 | 2011-07-05 | Sigma-Aldrich Co. | Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent |
US7932034B2 (en) * | 2006-12-20 | 2011-04-26 | The Board Of Trustees Of The Leland Stanford Junior University | Heat and pH measurement for sequencing of DNA |
JP4193997B1 (ja) * | 2007-12-05 | 2008-12-10 | プレシジョン・システム・サイエンス株式会社 | 新規dnaポリメラーゼ |
WO2009091847A2 (en) * | 2008-01-14 | 2009-07-23 | Life Technologies Corporation | Compositions, methods and systems for single molecule sequencing |
GB2461128B (en) * | 2008-06-25 | 2010-12-15 | Ion Torrent Systems Inc | ChemFET Arrays for Nucleic Acid Sequencing |
GB2461127B (en) | 2008-06-25 | 2010-07-14 | Ion Torrent Systems Inc | Methods and apparatus for measuring analytes using large scale FET arrays |
US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US8546128B2 (en) | 2008-10-22 | 2013-10-01 | Life Technologies Corporation | Fluidics system for sequential delivery of reagents |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US8603792B2 (en) * | 2009-03-27 | 2013-12-10 | Life Technologies Corporation | Conjugates of biomolecules to nanoparticles |
US8673627B2 (en) | 2009-05-29 | 2014-03-18 | Life Technologies Corporation | Apparatus and methods for performing electrochemical reactions |
EP2539471B1 (en) * | 2010-02-26 | 2014-08-06 | Life Technologies Corporation | Method for sequencing using a modified polymerase |
-
2011
- 2011-02-25 EP EP11748135.8A patent/EP2539471B1/en active Active
- 2011-02-25 US US13/035,081 patent/US20110262903A1/en not_active Abandoned
- 2011-02-25 US US13/035,177 patent/US20110318748A1/en not_active Abandoned
- 2011-02-25 WO PCT/US2011/026219 patent/WO2011106629A2/en active Application Filing
- 2011-02-25 EP EP14179490.9A patent/EP2808401B1/en active Active
- 2011-02-25 AU AU2011220536A patent/AU2011220536B2/en active Active
- 2011-02-25 WO PCT/US2011/026228 patent/WO2011106634A2/en active Application Filing
- 2011-02-28 LT LTEP15192848.8T patent/LT3040424T/lt unknown
- 2011-02-28 WO PCT/US2011/026468 patent/WO2011106770A2/en active Application Filing
- 2011-02-28 EP EP11748228.1A patent/EP2539473B8/en active Active
- 2011-02-28 WO PCT/US2011/026450 patent/WO2011106766A2/en active Application Filing
- 2011-02-28 EP EP15192848.8A patent/EP3040424B1/en active Active
- 2011-02-28 CN CN201180018980.2A patent/CN103097547B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
EP3040424B1 (en) | 2018-01-10 |
CN103097547A (zh) | 2013-05-08 |
EP2539471A4 (en) | 2013-03-13 |
EP3040424A1 (en) | 2016-07-06 |
WO2011106634A3 (en) | 2012-05-03 |
EP2808401B1 (en) | 2016-12-14 |
US20110318748A1 (en) | 2011-12-29 |
WO2011106634A2 (en) | 2011-09-01 |
EP2539473A2 (en) | 2013-01-02 |
WO2011106770A3 (en) | 2012-04-05 |
EP2539471B1 (en) | 2014-08-06 |
WO2011106766A2 (en) | 2011-09-01 |
EP2539473B1 (en) | 2015-11-04 |
WO2011106766A3 (en) | 2012-04-26 |
AU2011220536B2 (en) | 2012-05-24 |
EP2539473A4 (en) | 2013-03-13 |
AU2011220536A1 (en) | 2011-12-01 |
EP2539471A2 (en) | 2013-01-02 |
EP2539473B8 (en) | 2016-03-30 |
WO2011106629A3 (en) | 2012-01-19 |
WO2011106770A2 (en) | 2011-09-01 |
EP2808401A1 (en) | 2014-12-03 |
WO2011106629A2 (en) | 2011-09-01 |
CN103097547B (zh) | 2018-06-05 |
US20110262903A1 (en) | 2011-10-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP2539473A4 (en) | MODIFIED PROTEINS AND METHODS OF MAKING AND USING THE SAME | |
HK1255608A1 (zh) | 抗htra1抗體及使用方法 | |
HK1199892A1 (en) | Modified mini-hepcidin peptides and methods of using thereof | |
HK1182117A1 (zh) | 抗生腱蛋白- 抗體及使用方法 | |
ZA201304153B (en) | Anti-pcsk9 antibodies and methods of use | |
EP2582729A4 (en) | ANTI-AXL ANTIBODIES AND METHOD FOR THEIR USE | |
IL222961A0 (en) | Novel dna-dinding proteins and uses thereof | |
EP2529079A4 (en) | SELF-HARDENING HIGH STRENGTH SUPPORT AND MANUFACTURING METHOD THEREFOR | |
EP2427479A4 (en) | ANTIBODIES AND METHODS OF USE THEREOF | |
EP2635111A4 (en) | STABILIZED OPSIN PROTEINS WITH STAGE FUNCTION AND METHOD OF USE THEREOF | |
EP2547771A4 (en) | PRODUCTION OF PROTEINS AND POLYPEPTIDES | |
SG10201506782XA (en) | Notum protein modulators and methods of use | |
EP2611832A4 (en) | ANTI-CXCL13 ANTIBODIES AND METHOD FOR THEIR USE | |
EP2651964A4 (en) | NETWORKED PEPTIDES AND PROTEINS, METHOD FOR THEIR PREPARATION AND USES THEREOF | |
IL215795A0 (en) | Anti-il-17f antibodies and methods of use thereof | |
ZA201403760B (en) | Anti-cd98 antibodies and methods of use thereof | |
HK1201843A1 (en) | Anti-polyubiquitin antibodies and methods of use | |
EP2485678A4 (en) | COFERONS AND METHODS OF MAKING AND USING THE SAME | |
EP2536283A4 (en) | PHENYLHETEROARYL DERIVATIVES AND USE METHOD THEREFOR | |
EP2561072A4 (en) | NANOZYMES, PROCESSES FOR PRODUCING NANOZYMES, AND METHODS OF USING NANOZYMES | |
EP2593797A4 (en) | NEW METHODS OF PROTEIN EVOLUTION | |
EP2558496A4 (en) | ANTIBODIES TO POLYUBIQUITINE AND METHOD FOR THEIR USE | |
EP2655410A4 (en) | ANTI-INFLAMMATORY PROTEINS AND METHODS OF PREPARATION AND USE THEREOF | |
ZA201300174B (en) | Anti-neuropilin antibodies and methods of use | |
HK1189501A1 (zh) | 抗體組合物及使用方法 |