KR970707294A - 녹말의 당화에서 단당류 수준을 증가시키는 방법 및 이에 유용한 효소(a method for increasing monosaccharide levels in the saccharification of starch and enzymes useful therefor) - Google Patents
녹말의 당화에서 단당류 수준을 증가시키는 방법 및 이에 유용한 효소(a method for increasing monosaccharide levels in the saccharification of starch and enzymes useful therefor) Download PDFInfo
- Publication number
- KR970707294A KR970707294A KR1019970702751A KR19970702751A KR970707294A KR 970707294 A KR970707294 A KR 970707294A KR 1019970702751 A KR1019970702751 A KR 1019970702751A KR 19970702751 A KR19970702751 A KR 19970702751A KR 970707294 A KR970707294 A KR 970707294A
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- South Korea
- Prior art keywords
- maltulose
- enzyme preparation
- enzyme
- hydrolyzing
- rti
- Prior art date
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- 108090000790 Enzymes Proteins 0.000 title claims abstract 19
- 102000004190 Enzymes Human genes 0.000 title claims abstract 19
- 229920002472 Starch Polymers 0.000 title claims abstract 5
- 238000000034 method Methods 0.000 title claims abstract 5
- 235000019698 starch Nutrition 0.000 title claims abstract 5
- 239000008107 starch Substances 0.000 title claims abstract 5
- 150000002772 monosaccharides Chemical class 0.000 title abstract 2
- NBGXQZRRLOGAJF-UHFFFAOYSA-N Maltulose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)(CO)OCC1O NBGXQZRRLOGAJF-UHFFFAOYSA-N 0.000 claims abstract 14
- JCQLYHFGKNRPGE-HFZVAGMNSA-N maltulose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JCQLYHFGKNRPGE-HFZVAGMNSA-N 0.000 claims abstract 14
- 238000002360 preparation method Methods 0.000 claims abstract 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract 4
- 239000008121 dextrose Substances 0.000 claims abstract 4
- 230000007062 hydrolysis Effects 0.000 claims abstract 3
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract 3
- 239000004382 Amylase Substances 0.000 claims abstract 2
- 238000002523 gelfiltration Methods 0.000 claims description 2
- 230000003301 hydrolyzing effect Effects 0.000 claims 6
- 239000006188 syrup Substances 0.000 claims 5
- 238000000855 fermentation Methods 0.000 claims 3
- 230000004151 fermentation Effects 0.000 claims 3
- 229920001353 Dextrin Polymers 0.000 claims 2
- 239000004375 Dextrin Substances 0.000 claims 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 2
- 235000019425 dextrin Nutrition 0.000 claims 2
- 244000005700 microbiome Species 0.000 claims 2
- 235000020357 syrup Nutrition 0.000 claims 2
- 102000013142 Amylases Human genes 0.000 claims 1
- 108010065511 Amylases Proteins 0.000 claims 1
- 241000228212 Aspergillus Species 0.000 claims 1
- 241000228245 Aspergillus niger Species 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- 241000220317 Rosa Species 0.000 claims 1
- 241000223259 Trichoderma Species 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 235000019418 amylase Nutrition 0.000 claims 1
- 239000007864 aqueous solution Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 abstract 1
- 108090000637 alpha-Amylases Proteins 0.000 abstract 1
- 150000001720 carbohydrates Chemical class 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 1
- 230000013595 glycosylation Effects 0.000 abstract 1
- 238000006206 glycosylation reaction Methods 0.000 abstract 1
- 239000012506 Sephacryl® Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01022—Alpha-galactosidase (3.2.1.22)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
본 발명은 녹말에서 덱스트로즈를 상업적으로 생산하는 동안, 아밀로글루코시다제, 풀루라나제 또는 α-아밀라제의 효소들에 의하여 가수분해될 수 없는 아당류 말툴로즈를 제조하는 방법에 관한다. 본 발명은 말툴로즈 가수 분해용 효소 제조물에 관하여 기술하고 있다.
말툴로즈를 효소 가수 분해시키는 방법은 녹말 당화 과정중 단당류 수준을 증가시키기 위한 신규 및 추가의 방법을 제공한다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 Sephacryl 5200 HR OD(OD 280에서)과 용리 시간에 대한 α-갈락토시다제의 겔 여과법의 크로마토그램을 나타낸다.
Claims (14)
- 말툴로즈를 가수 분해시킬 수 있는 효소를 포함하는 효소 제조물로서, 상기 효소 제조물은 말툴로즈를 가수 분해시킬 수 있는 효소가 보강된 효소 제조물.
- 제1항에 있어서, 미생물에서 얻어진 말툴로즈를 가수 분해할 수 있는 효소 제조물.
- 제2항에 있어서, 상기 미생물이 곰팡이인 효소 제조물.
- 제3항에 있어서, 상기 곰팡이가 아스퍼질러스(Aspergillus) 균주 또는 트리코더마(Trichoderma) 균주인 효소 제조물.
- 제4항에 있어서, 상기 효소 제조물이 겔 여과에 의하여 분자량을 측정한 결과 약 132KD인 아스퍼질러스 나이저(Aspergillus niger)에서 얻은 말툴라제 효소를 포함하는 효소 제조물.
- 말툴라제 가수 분해용 제조물의 용도.
- 말툴로즈 가수 분해용 제1항의 효소 제조물의 용도.
- 상기 말툴로즈를 가수 분해시키는 효소 제조물을 사용하는 것을 특징으로 하는 말툴로즈 가수 분해 방법.
- (a) 덱스트로즈, 이소-시럽 및/또는 덱스트린을 함유하는 수성용액을 제조하는 단계; (b) 상기 욕액에 말툴로즈를 가수 분해시킬 수 있는 효소를 포함하는 효소 제조물을 가하는 단계〔상기 효소 제조물은 말툴로즈를 가수분해시킬 수 있는 활성이 강화된〕; 및 (c) 단계 (b)에서 얻은 용액을 말툴로즈를 가수 분해시키기에 적당한 조건하에서 1시간 동안 인큐베이팅시키는 단계를 포함하는 방법으로서, 덱스트로즈, 이소-시럽, 및/또는 말툴로즈 함유 덱스트린을 포함하는 용액으로부터 글루코즈를 제조하는 방법.
- 제9항에 있어서, 풀루라나제(pullanase), 산성 아밀라제(acid amylase), 또는 이들의 혼합물로 추가로 포함하는 방법.
- 제7항에 의하여 제조된 덱스트로즈 시럽을 이용하는 것을 포함하는 발효 공정.
- 에탄올을 발효에 의하여 생성하는 제11항의 발효 공정.
- 녹말에서 얻을 수 있으며 실질적으로 말툴로즈가 제거된 텍스트로즈 시럽.
- 녹말에서 얻을 수 있으며 실질적으로 말툴로즈가 제거된 이소-시럽.※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP94203124.6 | 1994-10-27 | ||
| EP94203124 | 1994-10-27 | ||
| PCT/US1995/015861 WO1996041485A2 (en) | 1995-06-07 | 1995-12-07 | Pipelined multiplexing for a multiport memory |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR970707294A true KR970707294A (ko) | 1997-12-01 |
Family
ID=8217318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019970702751A KR970707294A (ko) | 1994-10-27 | 1995-10-26 | 녹말의 당화에서 단당류 수준을 증가시키는 방법 및 이에 유용한 효소(a method for increasing monosaccharide levels in the saccharification of starch and enzymes useful therefor) |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0787202A1 (ko) |
| JP (1) | JPH10512741A (ko) |
| KR (1) | KR970707294A (ko) |
| CN (1) | CN1166860A (ko) |
| AU (1) | AU4278896A (ko) |
| CA (1) | CA2203812A1 (ko) |
| FI (1) | FI971782A0 (ko) |
| WO (1) | WO1996013602A1 (ko) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3400306A1 (en) * | 2016-01-05 | 2018-11-14 | Cargill, Incorporated | Method for fermenting sugars |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0140410B2 (en) * | 1983-09-11 | 1996-12-04 | Gist-Brocades N.V. | Novel enzyme product and its use in the saccharification of starch |
| US4717662A (en) * | 1985-01-31 | 1988-01-05 | Miles Laboratories, Inc. | Thermal stabilization of alpha-amylase |
| CA1283376C (en) * | 1986-07-09 | 1991-04-23 | John O. Carroll | .alpha.-AMYLASE MIXTURES FOR STARCH LIQUEFACTION |
| US5231017A (en) * | 1991-05-17 | 1993-07-27 | Solvay Enzymes, Inc. | Process for producing ethanol |
-
1995
- 1995-10-26 WO PCT/US1995/013879 patent/WO1996013602A1/en not_active Application Discontinuation
- 1995-10-26 CN CN95196461A patent/CN1166860A/zh active Pending
- 1995-10-26 EP EP95941339A patent/EP0787202A1/en not_active Withdrawn
- 1995-10-26 CA CA002203812A patent/CA2203812A1/en not_active Abandoned
- 1995-10-26 JP JP8514742A patent/JPH10512741A/ja active Pending
- 1995-10-26 KR KR1019970702751A patent/KR970707294A/ko not_active IP Right Cessation
- 1995-10-26 AU AU42788/96A patent/AU4278896A/en not_active Abandoned
-
1997
- 1997-04-25 FI FI971782A patent/FI971782A0/fi unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0787202A1 (en) | 1997-08-06 |
| FI971782A (fi) | 1997-04-25 |
| CN1166860A (zh) | 1997-12-03 |
| FI971782A0 (fi) | 1997-04-25 |
| JPH10512741A (ja) | 1998-12-08 |
| CA2203812A1 (en) | 1996-05-09 |
| AU4278896A (en) | 1996-05-23 |
| WO1996013602A1 (en) | 1996-05-09 |
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