KR970061259A - Red blood cells whose cohesion is reduced by chemical modification and a method for producing the same - Google Patents

Red blood cells whose cohesion is reduced by chemical modification and a method for producing the same Download PDF

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Publication number
KR970061259A
KR970061259A KR1019960002440A KR19960002440A KR970061259A KR 970061259 A KR970061259 A KR 970061259A KR 1019960002440 A KR1019960002440 A KR 1019960002440A KR 19960002440 A KR19960002440 A KR 19960002440A KR 970061259 A KR970061259 A KR 970061259A
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KR
South Korea
Prior art keywords
erythrocytes
blood cells
red blood
blood type
chemically modified
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KR1019960002440A
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Korean (ko)
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KR100193746B1 (en
Inventor
변시명
정성태
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윤덕용
한국과학기술원
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Priority to KR1019960002440A priority Critical patent/KR100193746B1/en
Priority to CA002279978A priority patent/CA2279978A1/en
Priority to AU15552/97A priority patent/AU1555297A/en
Priority to EP97901754A priority patent/EP0901521B1/en
Priority to DE69734511T priority patent/DE69734511T2/en
Priority to PCT/IB1997/000139 priority patent/WO1997028254A1/en
Priority to JP52744797A priority patent/JP4469025B2/en
Publication of KR970061259A publication Critical patent/KR970061259A/en
Application granted granted Critical
Publication of KR100193746B1 publication Critical patent/KR100193746B1/en

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Abstract

본 발명은 적혈구 표면의 혈액형 항원 및 조직특이성 항원 주위에 메톡시폴리에틸렌글리콜(methoxy-polyethylene glycol)의 비독성 폴리머를 공유결합시키는 화학수정(chemical modification)을 통하여, 혈액형 항체 및 조직특이성 항체의 접근을 공간적으로 방해함으로써, 항원-항체 반응에 의한 응집현상이 억제된 적혈구 및 그의 제조방법에 관한 것이다. 화학수정된 적혈구는 사람 또는 동물 유래의 적혈구를 0.9%NaCl 식염수로 희석한 용액의 pH를 9 내지 10으로 유지하면서, 활성화된 메톡시폴리에틸렌글리콜을 첨가하여 전기 적혈구와 반응시키는 방법에 의해 제조된다. 본 발명의 방법으로 화학수정된 적혈구는 혈액형에 관계없이 사용가능하며 충분한 산소전달능력도 갖고 있어, 응급수혈 및 이식장기의 보관 등 적혈구가 사용되는 모든 의학분야에 이용될 수 있다. 또한, 본 발명의 화학수정 적혈구의 제조방법은 혈액형에 관계없이 모든 혈액형 항원에 적용할 수 있고, 매우 단순하고 경제적이어서 차후 대량생산을 위한 반응기의 설계에도 매우 유리하다.The present invention relates to a method for the preparation of blood type antibodies and tissue specific antibodies through chemical modification that covalently attaches non-toxic polymers of methoxy-polyethylene glycol around blood type antigens and tissue specific antigens on the surface of erythrocytes Thereby preventing aggregation due to an antigen-antibody reaction, and a method for producing the same. Chemically modified red blood cells are prepared by reacting erythrocytes derived from human or animal with erythrocytes by adding activated methoxypolyethylene glycol while maintaining the pH of the solution diluted with 0.9% NaCl saline at 9 to 10. The chemically modified red blood cells in the method of the present invention can be used regardless of blood type and also have sufficient oxygen transfer ability and can be used in all medical fields in which erythrocytes are used such as emergency blood transfusion and storage of transplant organs. In addition, the method for producing chemically modified erythrocytes of the present invention can be applied to all blood type antigens regardless of blood type, and is very simple and economical, which is very advantageous in designing a reactor for subsequent mass production.

Description

화학수정에 의해 응집성이 감소된 적혈구 및 그의 제조방법Red blood cells whose cohesion is reduced by chemical modification and a method for producing the same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.

Claims (6)

적혈구를 식염수로 희석한 용액의 pH를 9 내지 10으로 유지하면서, 활성화된 메톡시폴리에틸렌글리콜(methoxy-polyethylene glycol)을 첨가하여 전기 적혈구와 반응시키는 단계를 포함하는 화학수정된(chemically modified) 적혈구의 제조방법.The method comprising the step of reacting with erythrocytes by adding activated methoxy-polyethylene glycol while maintaining the pH of the diluted solution of erythrocytes in saline at 9 to 10, Gt; 제1항에 있어서, 적혈구는 혈액형에 관계없이 사람 또는 동물 유래인 것을 특징으로 하는 화학수정된 적혈구의 제조방법.The method for producing chemically modified red blood cells according to claim 1, wherein the red blood cells are derived from human or animal regardless of blood type. 제1항에 있어서, 메톡시폴리에틸렌글리콜은 그의 한쪽 말단에 있는 수산화기가 시아누릭 클로라이드(cyanuric chloride)에 의해 활성화된 것을 특징으로 하는 화학수정된 적혈구의 제조방법.The method of claim 1, wherein the methoxy polyethylene glycol is activated by hydroxyl groups at one end thereof with cyanuric chloride. 제1항에 있어서, 적혈구는 0.9% NaCl 식염수에 약 2%(v/v) 농도가 되도록 희석하고, 메톡시폴리에틸렌글리콜은 전기 적혈구가 희석된 식염수에 1mM 내지 10mM로 첨가하는 것을 특징으로 하는 화학수정된 적혈구의 제조방법.2. The method according to claim 1, wherein the erythrocytes are diluted to a concentration of about 2% (v / v) in 0.9% NaCl saline and the methoxypolyethylene glycol is added to saline diluted with erythrocytes at 1 mM to 10 mM Method of manufacturing modified red blood cells. 제1항에 있어서, 메톡시폴리에틸렌글리콜은 2,000 내지 8,000의 분자량을 가지는 것을 특징으로 하는 화학수정된 적혈구의 제조방법.The method according to claim 1, wherein the methoxy polyethylene glycol has a molecular weight of 2,000 to 8,000. 적혈구를 식염수로 희석한 용액의 pH를 9 내지 10으로 유지하면서, 활성화된 메톡시폴리에틸렌글리콜을 첨가하여 전기 적혈구와 반응시켜 제조된 화학수정 적혈구.A chemically modified erythrocyte prepared by adding activated methoxy polyethylene glycol and reacting with erythrocytes while maintaining the pH of the diluted solution of erythrocytes in saline at 9 to 10. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed by the contents of the first application.
KR1019960002440A 1996-02-01 1996-02-01 Erythrocytes with reduced cohesiveness by chemical fertilization and manufacturing method thereof KR100193746B1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
KR1019960002440A KR100193746B1 (en) 1996-02-01 1996-02-01 Erythrocytes with reduced cohesiveness by chemical fertilization and manufacturing method thereof
CA002279978A CA2279978A1 (en) 1996-02-01 1997-02-03 Antigenic modulation of cells
AU15552/97A AU1555297A (en) 1996-02-01 1997-02-03 Antigenic modulation of cells
EP97901754A EP0901521B1 (en) 1996-02-01 1997-02-03 Antigenic modulation of cells
DE69734511T DE69734511T2 (en) 1996-02-01 1997-02-03 ANTIGENIC MODULATION OF CELLS
PCT/IB1997/000139 WO1997028254A1 (en) 1996-02-01 1997-02-03 Antigenic modulation of cells
JP52744797A JP4469025B2 (en) 1996-02-01 1997-02-03 Immune regulation by cell antigens

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019960002440A KR100193746B1 (en) 1996-02-01 1996-02-01 Erythrocytes with reduced cohesiveness by chemical fertilization and manufacturing method thereof

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KR970061259A true KR970061259A (en) 1997-09-12
KR100193746B1 KR100193746B1 (en) 1999-06-15

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