KR960029789A - 생체내 물질의 면역학적 분석용 키트 및 면역학적 분석 방법 - Google Patents
생체내 물질의 면역학적 분석용 키트 및 면역학적 분석 방법 Download PDFInfo
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- KR960029789A KR960029789A KR1019960001537A KR19960001537A KR960029789A KR 960029789 A KR960029789 A KR 960029789A KR 1019960001537 A KR1019960001537 A KR 1019960001537A KR 19960001537 A KR19960001537 A KR 19960001537A KR 960029789 A KR960029789 A KR 960029789A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
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- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
본 발명은 프로존 현상을 유도하지 않고서 생체내 물질의 편리한 분석이 가능한 면역분석 키트 및 면역분석 방법을 제공한다. 면역분석 키트는 분석 물질에 면역학적으로 결합하는 물질로 고정화시킨 자기성 입자(A), 및 분석 물질로 고정화시킨 비자기성 입자(B)를 적어도 함유한다. 면역분석 키트는 샘플 중의 분석 방해 물질에 면역학적으로 결합하는 물질로 고정화시킨 자기성 입자(C)를 추가로 함유할 수 있다. 샘플을 입자(A) 및 (C)와, 입자(B)와 반응시키고; 반응하지 못한 면역복합체 형태인 자기성 입자를 수집하고; 반응ㆍ용액에 남은 비자기성 입자의 흡광도를 측정함으로써 본 발명의 면역분석 방법을 수행한다. 샘플 중의 분석 물질은 분석 물질의 표준 샘플을 사용하여 제조된 보정 곡선을 참조로 하여 정량적으로 측정한다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제2도는 샘플 중의 분석물질 항원, 및 항원으로 고정화시킨 비자기성 입자가 경쟁적으로 반응하는 본 발명의 면역분석의 공정을 나타내는 도식이다.
Claims (4)
- 분석 물질에 면역학적으로 결합하는 물질로 고정화시킨 불용성 자기성 입자, 및 분석 물질로 고정화시킨, 특정 범위의 흡수 파장을 갖는 불용성 비자기성 입자를 함유함을 특징으로 하여 항원-항체 반응을 이용하여 샘플 중의 분석용 생체내물질을 면역학적으로 분석하는 키트.
- 제1항에 있어서, 분석을 방해하는 샘플 중의 물질에 면역학적으로 결합하는 물질로 고정화시킨 불용성 자기성 입자를 추가로 함유하는 분석용 생체내 물질을 면역학적으로 분석하는 키트.
- (1) 분석 물질에 면역학적으로 결합하는 물질로 고정화시킨 불용성 자기성 입자의 분산액 및 분석 물질로 고정화시킨, 특정 범위의 흡수 파장을 갖는 불용성 비자기성 입자의 분산액을 임의의 순서로 샘플과 혼합하여 혼합물을 제조하고; (2) 상기 혼합물에 자기장을 적용하여 면역학적으로 결합하지 못한 자기성 입자와 함께 상기 혼합물 중에 형성된 상기 자기성 입자와 상기 분석 물질의 복합체를 제거하고; (3) 흡광도를 측정함으로써 혼합물에 남은 상기 비자기성 입자의 농도를 측정하는 단계를 포함함을 특징으로 하여, 항원-항체 반응을 이용하여 샘플 중의 분석용 생체내 물질을 분석하는 방법.
- 제3항에 있어서, 분석을 방해하는 샘플 중의 물질에 면역학적으로 결합하는 물질로 고정화시킨 불용성 자기성 입자를 상기 혼합물에 추가로 가하고; 상기 혼합물 중에 형성된 상기 자기성 입자 및 상기 분석 방해물질의 복합체를 자기장에 의해 추가로 제거함을 특징으로 하여 샘플 중의 분석용 생체내 물질을 분석하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10627/1995 | 1995-01-26 | ||
JP1062795 | 1995-01-26 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR960029789A true KR960029789A (ko) | 1996-08-17 |
Family
ID=11755462
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019960001537A KR960029789A (ko) | 1995-01-26 | 1996-01-24 | 생체내 물질의 면역학적 분석용 키트 및 면역학적 분석 방법 |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0724156A1 (ko) |
KR (1) | KR960029789A (ko) |
AU (1) | AU4213396A (ko) |
CA (1) | CA2168156A1 (ko) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101257527B1 (ko) * | 2005-05-02 | 2013-04-23 | 바이오스케일, 아이엔씨. | 음파 디바이스를 이용하여 분석물을 검출하는 방법 및 장치 |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5876935A (en) * | 1997-01-08 | 1999-03-02 | Dade Behring Inc. | Luminescent specific binding assay |
GB9812519D0 (en) * | 1998-06-10 | 1998-08-05 | Immunoporation Ltd | Treating cells |
US7640083B2 (en) | 2002-11-22 | 2009-12-29 | Monroe David A | Record and playback system for aircraft |
US6837171B1 (en) | 2002-04-29 | 2005-01-04 | Palmer/Snyder Furniture Company | Lightweight table with unitized table top |
US20030119203A1 (en) | 2001-12-24 | 2003-06-26 | Kimberly-Clark Worldwide, Inc. | Lateral flow assay devices and methods for conducting assays |
US7314763B2 (en) * | 2002-08-27 | 2008-01-01 | Kimberly-Clark Worldwide, Inc. | Fluidics-based assay devices |
US7432105B2 (en) * | 2002-08-27 | 2008-10-07 | Kimberly-Clark Worldwide, Inc. | Self-calibration system for a magnetic binding assay |
US7285424B2 (en) | 2002-08-27 | 2007-10-23 | Kimberly-Clark Worldwide, Inc. | Membrane-based assay devices |
US7247500B2 (en) | 2002-12-19 | 2007-07-24 | Kimberly-Clark Worldwide, Inc. | Reduction of the hook effect in membrane-based assay devices |
US7851209B2 (en) | 2003-04-03 | 2010-12-14 | Kimberly-Clark Worldwide, Inc. | Reduction of the hook effect in assay devices |
US20040197819A1 (en) | 2003-04-03 | 2004-10-07 | Kimberly-Clark Worldwide, Inc. | Assay devices that utilize hollow particles |
US7943395B2 (en) | 2003-11-21 | 2011-05-17 | Kimberly-Clark Worldwide, Inc. | Extension of the dynamic detection range of assay devices |
US20050112703A1 (en) | 2003-11-21 | 2005-05-26 | Kimberly-Clark Worldwide, Inc. | Membrane-based lateral flow assay devices that utilize phosphorescent detection |
US7713748B2 (en) | 2003-11-21 | 2010-05-11 | Kimberly-Clark Worldwide, Inc. | Method of reducing the sensitivity of assay devices |
US7943089B2 (en) | 2003-12-19 | 2011-05-17 | Kimberly-Clark Worldwide, Inc. | Laminated assay devices |
US7521226B2 (en) | 2004-06-30 | 2009-04-21 | Kimberly-Clark Worldwide, Inc. | One-step enzymatic and amine detection technique |
WO2010074265A1 (ja) | 2008-12-25 | 2010-07-01 | ユニバーサル・バイオ・リサーチ株式会社 | 検体の前処理方法、および生体関連物質の測定方法 |
EP2554990B1 (en) | 2010-03-31 | 2016-08-31 | Sekisui Medical Co., Ltd. | Measurement method using immunochromatography, test strip for immunochromatography, and measurement reagent kit for immunochromatography |
WO2016002728A1 (ja) | 2014-06-30 | 2016-01-07 | パナソニックヘルスケアホールディングス株式会社 | 試料分析用基板、試料分析装置、試料分析システムおよび磁性粒子を含む液体から液体を取り除く方法 |
WO2016002727A1 (ja) | 2014-06-30 | 2016-01-07 | パナソニックヘルスケアホールディングス株式会社 | 試料分析用基板、試料分析装置、試料分析システムおよび試料分析システム用プログラム |
EP3163306A4 (en) | 2014-06-30 | 2018-01-24 | Panasonic Healthcare Holdings Co., Ltd. | Substrate for sample analysis, and sample analysis apparatus |
US10520521B2 (en) | 2014-06-30 | 2019-12-31 | Phc Holdings Corporation | Substrate for sample analysis, sample analysis device, sample analysis system, and program for sample analysis system |
US10539583B2 (en) | 2014-12-12 | 2020-01-21 | Phc Holdings Corporation | Substrate for sample analysis, sample analysis device, sample analysis system, and program for sample analysis system |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0750113B2 (ja) * | 1988-12-02 | 1995-05-31 | 日本電信電話株式会社 | レーザ磁気免疫測定法 |
JPH05180842A (ja) * | 1991-11-08 | 1993-07-23 | Nippon Paint Co Ltd | 抗原・抗体反応による生体内物質の測定方法 |
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1996
- 1996-01-23 AU AU42133/96A patent/AU4213396A/en not_active Abandoned
- 1996-01-24 KR KR1019960001537A patent/KR960029789A/ko not_active Application Discontinuation
- 1996-01-24 EP EP96100988A patent/EP0724156A1/en not_active Withdrawn
- 1996-01-26 CA CA002168156A patent/CA2168156A1/en not_active Abandoned
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101257527B1 (ko) * | 2005-05-02 | 2013-04-23 | 바이오스케일, 아이엔씨. | 음파 디바이스를 이용하여 분석물을 검출하는 방법 및 장치 |
Also Published As
Publication number | Publication date |
---|---|
EP0724156A1 (en) | 1996-07-31 |
AU4213396A (en) | 1996-08-01 |
CA2168156A1 (en) | 1996-07-27 |
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