KR960014337A - Novel glutamic acid dehydrogenase (I) and preparation method thereof - Google Patents

Novel glutamic acid dehydrogenase (I) and preparation method thereof Download PDF

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Publication number
KR960014337A
KR960014337A KR1019940028255A KR19940028255A KR960014337A KR 960014337 A KR960014337 A KR 960014337A KR 1019940028255 A KR1019940028255 A KR 1019940028255A KR 19940028255 A KR19940028255 A KR 19940028255A KR 960014337 A KR960014337 A KR 960014337A
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KR
South Korea
Prior art keywords
glutamic acid
acid dehydrogenase
chromatography
preparation
bovine brain
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KR1019940028255A
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Korean (ko)
Inventor
조성우
최수영
이종원
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조성우
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Priority to KR1019940028255A priority Critical patent/KR960014337A/en
Publication of KR960014337A publication Critical patent/KR960014337A/en

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Abstract

본 발명은 소의 뇌로부터 분리한 분자량이 각기 57.5kDa인 동일한 단량체 6개로 구성된 약 330kDa의 글루탐산 탈수소효소(I) 및 그의 제조방법에 관한 것이다. 본 발명의 글루탐산 탈수소효소(GDH)는 GDH-결손 신경이상증세를 예방 및 치료할 수 있는 약제의 개발에 이용될 수 있을 것이다.The present invention relates to glutamic acid dehydrogenase (I) of about 330 kDa consisting of six identical monomers each having a molecular weight of 57.5 kDa isolated from bovine brain, and a method for preparing the same. Glutamic acid dehydrogenase (GDH) of the present invention can be used in the development of a medicament capable of preventing and treating GDH-defective neuropathy.

Description

신규한 글루탐산 탈수소효소(I) 및 그의 제조방법Novel glutamic acid dehydrogenase (I) and preparation method thereof

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (2)

소의 뇌로부터 제조된 분자량 약 330kDa의 다음과 같은 아미노산 조성을 가지는 글루탐산 탈수소효소(I) :Glutamic acid dehydrogenase (I) having the following amino acid composition of about 330 kDa prepared from bovine brain: (i) 소의 뇌를 파쇄하고 원심분리하여 상등액을 수득하는 고정; (ii) 전기 상등액을 음이온 교환수지 크로마토그래피하는 공정; (iii) 전기에서 얻은 활성분획을 하이드록시아파타이트 크로마토그래피하는 공정; (iv) 전기에서 얻은 활성분획을 친화성 크로마트그래피하는 공정; (v) 전기에서 얻은 활성분획을 소수성 크로마토그래피하는 공정; (vi) 전기에서 얻은 활성분획을 젤 여과 크로마토그래피하는 공정을 포함하는 제1항의 글루탐산 탈수소효소(I)를 제조하는 방법.(i) fixation to disrupt the bovine brain and centrifuge to obtain a supernatant; (ii) anion exchange resin chromatography of the supernatant; (iii) hydroxyapatite chromatography on the active fraction obtained in the preceding; (iv) affinity chromatography of the active fractions obtained from the foregoing; (v) hydrophobic chromatography of the active fractions obtained from the foregoing; (vi) A method for producing glutamic acid dehydrogenase (I) according to claim 1, comprising the step of performing gel filtration chromatography on the active fraction obtained in the previous period. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940028255A 1994-10-31 1994-10-31 Novel glutamic acid dehydrogenase (I) and preparation method thereof KR960014337A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940028255A KR960014337A (en) 1994-10-31 1994-10-31 Novel glutamic acid dehydrogenase (I) and preparation method thereof

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Application Number Priority Date Filing Date Title
KR1019940028255A KR960014337A (en) 1994-10-31 1994-10-31 Novel glutamic acid dehydrogenase (I) and preparation method thereof

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KR960014337A true KR960014337A (en) 1996-05-22

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200107121A (en) * 2019-03-06 2020-09-16 주식회사 엘지화학 Gas separation membrane, gas separation membrane module comprising the same and preparation method for the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200107121A (en) * 2019-03-06 2020-09-16 주식회사 엘지화학 Gas separation membrane, gas separation membrane module comprising the same and preparation method for the same

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