KR950014296A - Yeast Containing Organic Germanium and Its Manufacturing Method - Google Patents

Yeast Containing Organic Germanium and Its Manufacturing Method Download PDF

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Publication number
KR950014296A
KR950014296A KR1019930025595A KR930025595A KR950014296A KR 950014296 A KR950014296 A KR 950014296A KR 1019930025595 A KR1019930025595 A KR 1019930025595A KR 930025595 A KR930025595 A KR 930025595A KR 950014296 A KR950014296 A KR 950014296A
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South Korea
Prior art keywords
yeast
germanium
weight
culturing
medium
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KR1019930025595A
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Korean (ko)
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KR970002248B1 (en
Inventor
손창욱
송원종
이상철
오태광
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손창욱
대지실업 주식회사
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Priority to KR1019930025595A priority Critical patent/KR970002248B1/en
Priority to JP6030340A priority patent/JP2737636B2/en
Publication of KR950014296A publication Critical patent/KR950014296A/en
Priority to US08/654,039 priority patent/US5792646A/en
Application granted granted Critical
Publication of KR970002248B1 publication Critical patent/KR970002248B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

본 발명은 탈지대두단백, 효모추출물, 클루코스 및 물로 이루어진 기본배지에 효모균주에 대하여 게르마늄 6 내지 10중량%를 첨가한 후, 효모를 배양하는 단계와 기본배지에 상기 단계에서 보다 증가된 양의 게르마늄을 첨가시킨후, 이 배지에서 상기 단계에서 배양된 효모균주를 배양하는 단계와 상기 단계를 수회 반복하고 실시하는 단계와 상기 단계에서 선별된 효모를 기본배지에서 증식시킨 후, 효모의 생장 대기수에 게르마늄 및 글루코스를 첨가하여 배양하는 것으로 이루어진 다량의 유기게르마늄을 함유하는 효모의 제조방법에 관한 것이다.The present invention, after adding 6 to 10% by weight of germanium to the yeast strain to the basic medium consisting of skim soy protein, yeast extract, glucose and water, the step of culturing yeast and the increased amount of the base medium in the above step After adding germanium, culturing the yeast strain cultured in the above step in this medium, repeating and performing the above steps several times, and growing the yeast selected in the above step in the basic medium, and then waiting for the growth of the yeast It relates to a method for producing a yeast containing a large amount of organic germanium consisting of culturing by adding germanium and glucose.

Description

유기 게르마늄을 함유하는 효모 및 그의 제조방법Yeast Containing Organic Germanium and Its Manufacturing Method

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명에 따른 배양지들에서의 효모의 생장곡선을 나타낸 그래프이다.1 is a graph showing the growth curve of yeast in the culture medium according to the present invention.

제2도는 효모균체내로 유입된 무기 게르마늄과 유기태 전환을 확인하기 위하여 본 발명에 따른 효모용액과 무기 게르마늄의 투석결과를 나타낸 그래프이다.2 is a graph showing the dialysis results of the yeast solution and the inorganic germanium according to the present invention in order to confirm the conversion of the inorganic germanium and organic state introduced into the yeast cells.

Claims (4)

(1) 탈지대두단배, 효모추출물, 글르코스 및 물로 이루어진 기본배지에 효모균주에 대하여 게르마늄 6 내지 10중량%를 첨가한 후, 효모를 배양하는 단계와;(1) adding 6 to 10% by weight of germanium with respect to the yeast strain to the basic medium consisting of skim soybean meal, yeast extract, glucose and water, and then culturing the yeast; (2) 기본배지에 상기 단계에서 보다 증가된 양의 게르마늄을 첨가시킨후, 이 배지에서 상기 단계에서 배양된 효모균주를 배양하는 단계와;(2) adding to the basal medium an increased amount of germanium in the above step, and then culturing the yeast strain cultured in the above step in the medium; (3) 상기 단계를 수회 반복 실시하는 단계와;(3) repeating the above steps several times; (4) 상기 단계에서 선별된 효모를 기본배지에서 증식시킨 후, 효모의 생장대수기에 게르마늄 및 글루코스를 첨가하여 배양하는 것으로 이루어진 다량의 유기 게르마늄을 함유하는 효모의 제조방법.(4) A method for producing yeast containing a large amount of organic germanium consisting of culturing the yeast selected in the above step is grown in the basal medium, followed by adding germanium and glucose to the growth log of the yeast. 제1항에 있어서, 상기 기본 배지는 탈지대두단백 4.2 내지 5.2중량%, 효모추출물 0.5 내지 0.7중량%, 글로코스 6.5 내지 7.5중량%와 나머지 물로 이어지는 것임을 특징으로 하는 다량의 유기 게르마늄을 함유하는 효모의 제조방법.The yeast containing a large amount of organic germanium according to claim 1, wherein the basal medium leads to 4.2 to 5.2% by weight of defatted soy protein, 0.5 to 0.7% by weight of yeast extract, 6.5 to 7.5% by weight of glycos, and the remaining water. Manufacturing method. 제1항에 있어서, 효모의 생장대수기에 전체 배지조성물에 대하여 게르마늄은 0.01 내지 0.1중량%, 글루코스는 5 내지 7중량%를 첨가하는 것임을 특징으로 하는 다량의 유기 게르마늄을 함유하는 효모의 제조방법.The method for preparing yeast containing a large amount of organic germanium according to claim 1, wherein germanium is added in an amount of 0.01 to 0.1% by weight and glucose is added in an amount of 5 to 7% by weight based on the total medium composition. . 제1항 내지 제3항의 방법에 따라 제조된 다량의 유기 게르마늄을 함유하는 효모.Yeast containing a large amount of organic germanium prepared according to the method of claims 1 to 3. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930025595A 1993-11-29 1993-11-29 Processing process of yeast containing organic germanium KR970002248B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
KR1019930025595A KR970002248B1 (en) 1993-11-29 1993-11-29 Processing process of yeast containing organic germanium
JP6030340A JP2737636B2 (en) 1993-11-29 1994-02-28 Yeast containing organogermanium and method for producing the same
US08/654,039 US5792646A (en) 1993-11-29 1996-05-28 Process for preparing S. cerevisiae containing organically bound germanium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930025595A KR970002248B1 (en) 1993-11-29 1993-11-29 Processing process of yeast containing organic germanium

Publications (2)

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KR950014296A true KR950014296A (en) 1995-06-15
KR970002248B1 KR970002248B1 (en) 1997-02-26

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JP (1) JP2737636B2 (en)
KR (1) KR970002248B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100863604B1 (en) * 2007-04-02 2008-10-15 김현수 A microbial agent for solubilizing inorganic germanium and a method for producing agricultural products comprising germanium by using the same

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2008047596A1 (en) * 2006-10-18 2010-02-25 不二製油株式会社 Freezing-resistant yeast
KR101178205B1 (en) * 2008-12-04 2012-08-29 게란티제약 주식회사 Ethanol Resistance Saccharomyces cerevisiae GB-01 by Protoplast Fusion, Method for Manufacturing thereof, Method for Manufacturing Yeast Containing High Content of Bio Organic Germanium by using Saccharomyces cerevisiae GP-01 and high water soluble sodium metagermanate as a germanium source
WO2012090554A1 (en) 2010-12-28 2012-07-05 合同会社パラ微生物研究所 Novel method for culturing microorganism, method for producing cells of microorganism having novel elemental composition, and microorganism produced thereby
KR102070168B1 (en) * 2018-04-30 2020-01-28 주식회사 벤스랩 Organic germanium assisted yeast fermentated material having skin whitening, skin antiwrinkle and antiinflammation effect
EP4098105A4 (en) * 2020-01-28 2024-02-21 Gang, Yoon Young Method for cultivating functional crops using nano organic germanium and nano organic selenium

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100863604B1 (en) * 2007-04-02 2008-10-15 김현수 A microbial agent for solubilizing inorganic germanium and a method for producing agricultural products comprising germanium by using the same

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Publication number Publication date
JP2737636B2 (en) 1998-04-08
KR970002248B1 (en) 1997-02-26
JPH07194370A (en) 1995-08-01

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