KR950003438A - Preparation and Separation and Purification Methods of Resin Strain Streptomyces Ravendopolya DK-506 and Aclacinomycin A Using the Same - Google Patents

Preparation and Separation and Purification Methods of Resin Strain Streptomyces Ravendopolya DK-506 and Aclacinomycin A Using the Same Download PDF

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KR950003438A
KR950003438A KR1019930012506A KR930012506A KR950003438A KR 950003438 A KR950003438 A KR 950003438A KR 1019930012506 A KR1019930012506 A KR 1019930012506A KR 930012506 A KR930012506 A KR 930012506A KR 950003438 A KR950003438 A KR 950003438A
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strain
aclacinomycin
separation
same
ravendopolya
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KR1019930012506A
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Korean (ko)
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KR960014621B1 (en
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조원태
김완섭
김명국
박진규
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강재헌
동국제약 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C12R2001/56—Streptomyces lavendulae

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 항암제인 아클라시노마이신을 생산하는 스트랩토마이세스속 균주 및 이를 이용하여 아클라시노마이신을 제조하고 이를 분리정제하는 방법에 관한 것이다. 본 발명에 사용된 균주, 스트랩토마이세스 라벤도폴리아 DK-506(ATCC 8537P)은 돌연변이원 처리균주로서 전분이 함유된 발효배지에서 30℃, 700rpm의 호기적인 배양조건으로 배양했을 때 친주인 스트랩토마이세스 라벤도폴리아 ATCC 15872균주보다 2배 이상의 높은 아클라시노마이신 A를 생산하였으며 생산된 아클라시노마이신 A는 클로로포름과 메칠알콜을 20:1로 섞은 용매로 추출한 후 두차례에 걸친 컬럼 크로마토그라피와 HPLC를 사용하여 분리정제하였다.The present invention relates to a strain of Streptomyces genus that produces an anticancer agent, alaccinomycin, and a method for preparing and separating and purifying aclocinomycin using the same. The strain used in the present invention, straptomyces ravendofolia DK-506 (ATCC 8537P) is a murine-treated strain, which is a parent strain when incubated in aerobic culture conditions at 30 ° C. and 700 rpm in a fermentation medium containing starch. Alaccinomycin A produced more than twice as high as that of Tomeises Ravendofolia ATCC 15872 strain. Aclaccinomycin A was extracted with a solvent of 20: 1 mixture of chloroform and methyl alcohol, followed by two column chromatography. Separation and purification using graphigraphy and HPLC.

Description

신 균주 스트렙토마이세스 라벤도폴리아 DK-506 및 이를 이용한 아클라시노마이신 A의 제조와 분리정제 방법Preparation and Separation and Purification Methods of Resin Strain Streptomyces Ravendopolya DK-506 and Aclacinomycin A Using the Same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (4)

아클라시노마이신 A 생산능을 갖는 것을 특징으로 하는 스트렙토아마세스 라벤도폴리아 DK-506(KCTC8537P).Streptomases levodofolia DK-506 (KCTC8537P), characterized by having alaccinomycin A production capacity. 스트렙토마미세스 라벤도폴리아 DK-506을 영양배지에 배양하여 아클라시노마이신 A를 제조하고 생성된 아클라시노마이신 A를 분리정제하는 방법.Method of culturing Streptomyces rabendopolya DK-506 in a nutrient medium to prepare aclacinomycin A and to isolate and purify the resulting aclacinomycin A. 상기 제2항에 있어서 배양은 28-30℃, pH 6.8-7.5 범위의 호기적 조건에서 4-5일간 배양함을 특징으로 하는 아클라시노마이신 A의 제조방법.The method according to claim 2, wherein the culturing is carried out for 4-5 days under aerobic conditions in the range of 28-30 ° C and pH 6.8-7.5. 상기 제2항에 있어서 용제로 클로로포름과 메칠알콜을 20 : 1 내지 20 : 5의 부피 조성비로 사용하여 아클라시노마이신 A를 분리정제하는 방법.The method for separating and purifying aclocinomycin A according to claim 2 using chloroform and methyl alcohol as a solvent in a volume composition ratio of 20: 1 to 20: 5. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930012506A 1993-07-05 1993-07-05 Streptomyces lavendofoliae dk-506, and processing method of aclacinomycin-a KR960014621B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019930012506A KR960014621B1 (en) 1993-07-05 1993-07-05 Streptomyces lavendofoliae dk-506, and processing method of aclacinomycin-a

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930012506A KR960014621B1 (en) 1993-07-05 1993-07-05 Streptomyces lavendofoliae dk-506, and processing method of aclacinomycin-a

Publications (2)

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KR950003438A true KR950003438A (en) 1995-02-16
KR960014621B1 KR960014621B1 (en) 1996-10-19

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