KR930700651A - Thermostable protease derived from Thermococcus - Google Patents

Thermostable protease derived from Thermococcus

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Publication number
KR930700651A
KR930700651A KR1019920702952A KR920702952A KR930700651A KR 930700651 A KR930700651 A KR 930700651A KR 1019920702952 A KR1019920702952 A KR 1019920702952A KR 920702952 A KR920702952 A KR 920702952A KR 930700651 A KR930700651 A KR 930700651A
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KR
South Korea
Prior art keywords
thermococcus
protease
dsm accession
celer
stetteri
Prior art date
Application number
KR1019920702952A
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Korean (ko)
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KR100249995B1 (en
Inventor
안트라니키안 가라베트
크링게베르그 미하엘
Original Assignee
안네 제케르
노보 노르디스크 아크티에 셀스카브
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Priority claimed from DK145890A external-priority patent/DK145890D0/en
Application filed by 안네 제케르, 노보 노르디스크 아크티에 셀스카브 filed Critical 안네 제케르
Publication of KR930700651A publication Critical patent/KR930700651A/en
Application granted granted Critical
Publication of KR100249995B1 publication Critical patent/KR100249995B1/en

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Abstract

내용 없음.No content.

Description

터모코쿠스(Thermococcus)유래 열안정성 프로테아제Thermostable protease derived from Thermococcus

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도 내지 제4도는 Thermococcus celer; Thermococcus sp. AN1, Thermococcus stetteri; Thermococcus litoralis;로부터 획득한 프로테아제의 효소적 활성과 온도 및 pH와의 관계를 각기 나타낸다.1 through 4 show Thermococcus celer; Thermococcus sp. AN1, Thermococcus stetteri; The enzymatic activity of protease obtained from Thermococcus litoralis; and the relationship between temperature and pH are shown, respectively.

Claims (11)

(a) pH 6.0부터 pH10.0까지 범위의 최적 pH; (b) 75℃부터 100℃까지 범위의 최적온도; 의 특성을 갖는 것을 특징으로 하는 프로테아제.(a) an optimal pH ranging from pH 6.0 to pH10.0; (b) an optimum temperature in the range of 75 ° C. to 100 ° C .; Protease characterized in that it has the characteristics of. (a) pH 6.0 부터 pH 10.0까지 범위의 최적 pH; (b) 75℃부터 100℃까지 범위의 최적온도; (c) Thermococcus celer, DSM 기탁번호 2476, Thermococcus sp. AN1, DSM 기탁번호 2770, 또는 Thermococcus stetteri DSM 기탁번호 5262 또는 Thermococcus litorals, DSM 기탁번호 5474로 부터 유도된 프로테아제의 면역화학적 특성과 동일한 또는 부분적으로 동일한 면역화학적 성질; 을 갖는 특징으로 하는 프로테아제.(a) an optimal pH ranging from pH 6.0 to pH 10.0; (b) an optimum temperature in the range of 75 ° C. to 100 ° C .; (c) Thermococcus celer, DSM Accession No. 2476, Thermococcus sp. Immunochemical properties identical or partially identical to the immunochemical properties of the proteases derived from AN1, DSM Accession No. 2770, or Thermococcus stetteri DSM Accession No. 5262 or Thermococcus litorals, DSM Accession No. 5474; Protease characterized by having. (a) pH6.0부터 pH10.0까지 범위의 최적 pH; (b) 75℃부터 100℃까지 범위의 최적온도; (c) Thermococcus속 균주로 부터 유도된 것; 이라는 특성을 갖는 것을 특징으로 하는 프로테아제.(a) an optimal pH ranging from pH6.0 to pH10.0; (b) an optimum temperature in the range of 75 ° C. to 100 ° C .; (c) derived from strains of the genus Thermococcus; Protease characterized in that it has a property. 제3항에 있어서, 종 T. celer, Thermococcus sp. AN1, T.stetteri 또는 T.litoralis 균주로 부터 유도된 것을 특징으로 하는 프로테아제.The method of claim 3, wherein the species T. celer, Thermococcus sp. Protease, characterized in that derived from AN1, T.stetteri or T.litoralis strains. 제4항에 있어서, T.celer DSM 기탁번호 2476, 또는 Thermococcus sp. AN1, DSM 기탁번호 2770, 또는 Thermococcus stetteri, DSM 기탁번호 5262 또는 Thermococcus litoralis DSM 기탁번호 5474 균주로 부터 유도된 것을 특징으로 하는 프로테아제.The method of claim 4, wherein T.celer DSM Accession No. 2476, or Thermococcus sp. A protease, which is derived from AN1, DSM Accession No. 2770, or Thermococcus stetteri, DSM Accession No. 5262 or Thermococcus litoralis DSM Accession No. 5474. 탄소 및 질소원 그리고 무기염을 함유하는 적당한 영양 배지에서 프로테아제를 생산하는 Thermococcus 균주를 배양하고, 이여서 얻고자 하는 효소를 채취하는 것으로 구성된 방법으로 제1항 내지 제5항중 어느 한 항에 따른 프로테아제를 제제하는 방법.A protease according to any one of claims 1 to 5 is prepared by culturing a thermococcus strain producing a protease in a suitable nutrient medium containing a carbon and nitrogen source and an inorganic salt, and then extracting the enzyme to be obtained. How to. 제6항에 있어서, Thermococcus celer, Thermococcus sp. AN1, Thermococcus stetteri, 또는 Thermococcus litoralis 균주를 배양하는 것을 특징으로 하는 방법.According to claim 6, Thermococcus celer, Thermococcus sp. A method comprising culturing AN1, Thermococcus stetteri, or Thermococcus litoralis strains. 제7항에 있어서, Thermococcus celer, DSM 기탁번호 2476, Thermococcus sp. AN1, DSM 기탁번호 2770, Thermococcus stetteri, DSM 기탁번호 5262, 또는 Thermococcus litoralis, DSM 기탁번호 5474, 또는 이들의 돌연변이체 또는 변이체를 배양하는 것을 특징으로 하는 방법.The method of claim 7, wherein the Thermococcus celer, DSM Accession No. 2476, Thermococcus sp. AN1, DSM Accession No. 2770, Thermococcus stetteri, DSM Accession No. 5262, or Thermococcus litoralis, DSM Accession No. 5474, or a mutant or variant thereof. 제1항 내지 제5항중 어느 한 항에 따른 프로테아제를 포함하는 것을 특징으로 하는, 비-분진 과립체, 액체, 특이 안정화된 액체, 슬러리(Slurry), 또는 보호효소의 형태로된 단백질 가수분해 세제 첨가제.Proteolytic detergents in the form of non-dust granules, liquids, specific stabilized liquids, slurries, or protective enzymes, characterized in that they comprise a protease according to any one of claims 1 to 5. additive. 제1항 내지 제5항중 어느 한 항에 따른 프로테아제를 포함하는 것을 특징으로 하는 프로테아제-함유 세제 조성물.Protease-containing detergent composition comprising a protease according to any one of claims 1 to 5. 제10항에 있어서, 하나 또는 2이상의 다른 효소, 특히 아밀라제, 리파아제, 셀룰라제, 또는 피옥시다제를 더 포함하는 것을 특징으로 하는 세제 조성물.11. A detergent composition according to claim 10, further comprising one or two or more other enzymes, in particular amylase, lipase, cellulase, or oxidase. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019920702952A 1990-06-15 1991-06-14 Thermostable protease from thermococcus KR100249995B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK145890A DK145890D0 (en) 1990-06-15 1990-06-15 THERMOSTABLE PROTEASE
DK1458/90 1990-06-15
PCT/DK1991/000160 WO1991019792A1 (en) 1990-06-15 1991-06-14 Thermostable protease from thermococcus

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KR930700651A true KR930700651A (en) 1993-03-15
KR100249995B1 KR100249995B1 (en) 2000-03-15

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JP (1) JP3126729B2 (en)
KR (1) KR100249995B1 (en)
AT (1) ATE115624T1 (en)
DE (1) DE69105974T2 (en)
DK (2) DK145890D0 (en)
ES (1) ES2065695T3 (en)
FI (1) FI103669B1 (en)
WO (1) WO1991019792A1 (en)

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US5714373A (en) * 1995-02-27 1998-02-03 Recombinant Biocatalysis, Inc. Thermococcus AV4 and enzymes produced by the same
DE69615055T2 (en) 1995-12-12 2002-06-20 Takara Shuzo Co., Ltd. GENES CODING FOR ULTRATHERMOSTABLE PROTEASES
AU3455000A (en) * 1999-04-07 2000-11-14 Takara Shuzo Co., Ltd. Composition for decomposing protein
EP2468862A1 (en) 2010-12-23 2012-06-27 Qiagen GmbH Method and kit for processing wax-embedded biological samples
WO2016196202A1 (en) * 2015-05-29 2016-12-08 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
CN108603203A (en) * 2015-11-12 2018-09-28 诺维信公司 The low fermenting process that is soaked
KR102096063B1 (en) * 2018-06-18 2020-04-02 한국해양과학기술원 Producing Method of Media for Thermophilic Marine Bacteria Using Seawater and bay salt

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US3796635A (en) * 1972-12-26 1974-03-12 Monsanto Co Process for the preparation of heat resistant neutral protease enzyme
DK149867C (en) * 1979-08-08 1987-04-06 New Zealand Dev Finance PROTEOLYTIC ENZYME PREPARATION AND PROCEDURE FOR PRODUCING THEREOF
US5173403A (en) * 1989-02-24 1992-12-22 Oklahoma Medical Research Foundation Thermostable acid protease from sulfolobus acidocaldarius and gene

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