KR930004461A - 생 전분 소화 가능한 아밀라아제 - Google Patents

생 전분 소화 가능한 아밀라아제 Download PDF

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KR930004461A
KR930004461A KR1019920014523A KR920014523A KR930004461A KR 930004461 A KR930004461 A KR 930004461A KR 1019920014523 A KR1019920014523 A KR 1019920014523A KR 920014523 A KR920014523 A KR 920014523A KR 930004461 A KR930004461 A KR 930004461A
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pestallototiopsis
enzyme
ifo
fungus
sank
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KR1019920014523A
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아쓰시 하또리
미쓰요 다까하시
마사미 미우라
노리요시 우찌다
고우헤이 후루야
스요시 호소야
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가와무라 요시부미
상꾜 가부시끼가이샤
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Publication of KR930004461A publication Critical patent/KR930004461A/ko

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    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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    • C12R2001/645—Fungi ; Processes using fungi

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Abstract

내용 없음.

Description

생 전분 소화 가능한 아밀라아제
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 효소에 대한 pH-활성도 곡선을 나타내고,
제2도는 본 발명의 효소 pH와 안정성 간의 관계를 나타내고,
제3도는 본 발명의 효소에 대한 온도/활성도 곡선을 나타낸다.

Claims (19)

  1. 효소의 활성을 위한 최적 pH가 pH4∼5이고, pH3∼9의 범위내 37℃의 온도에서 안정함을 특징으로 하는 생 전분 가수분해 가능한 효소.
  2. 제1항에 있어서, pH3∼10의 범위내 37℃의 온도에서 안정한 효소.
  3. 제1항에 있어서, SDS폴리아크릴아미드 겔 전기영동에 의해 측정된 분자량이 약 82,000달톤인 효소.
  4. 제1항에 있어서, 페스탈로티오프시스 속의 진균류에서 수득되는 효소.
  5. 제4항에 있어서, 상기 진균류가 하기 종의 균주임을 특징으로 하는 효소, 페스탈로티오프시스 푸네레아, 페스탈로티오프시스 미크로스포라, 페스탈로티오프시스 아카시아에, 페스탈로티오프시스 크라시우슬라 또는 페스탈로티오프시스 네글렉타.
  6. 제5항에 있어서, 상기 진균류가 페스탈로티오프시스 푸네레아 IFO 5427(SANK 15174), 페스탈로티오프시스 미크로스포라 IFO 31056, 페스탈로티오프시스 아카시아에 IFO 31054, 페스탈로티오프시스 크라시우슬라 IFO 31055 또는 페스탈로티오프시스 네글렉타(SANK 13390)인 효소.
  7. 페스탈로티오프시스 속의 진균류를 배양한 다음, 얻어진 배양액으로부터 효소를 단리함을 특징으로 하는, 생 전분 가수분해 가능한 효소의 제조방법.
  8. 제7항에 있어서, 상기 진균류가 하기 종의 균주인 방법, 페스탈로티오프시스 푸네레아, 페스탈로티오프시스 미크로스포라, 페스탈로티오프시스 아카시앙에, 페스탈로티오프시스 크라시우슬라 또는 페스탈로티오프시스 네글렉타.
  9. 제8항에 있어서, 상기 진균류가 페스탈로티오프시스 푸네레아 IFO 5427(SANK 15174), 페스탈로티오프시스 미크로스포라 IFO 31056, 페스탈로티오프시스 아카시아에 IFO 31054, 페스탈로티오프시스 크라시우슬라 IFO 31055 또는 페스탈로티오프시스 네글렉타 (SANK 13390)인 효소.
  10. 제 8항에 있어서, 상기 진균류가 페스탈로티오프시스 푸네레아 IFO 5427 (SANK 15174)인 방법.
  11. 제7항 내지 10항 중 어느 한 항에 있어서, 20℃∼40℃의 온도에서 배양을 수행하는 방법.
  12. 제1항 내지 6항 중 어느 한 항에 따른 효소를 이용한 다당류의 분해 방법.
  13. 제12항에 있어서, 상기 다당류가 전분인 방법.
  14. 제12항 또는 13항에 있어서, 상기 다당류가 비가공된 다당류인 방법.
  15. 제7항 내지 14항 중 어느 한 항에 있어서, 10℃∼75℃의 온도에서 수행되는 방법.
  16. 제15항에 있어서, 상기 온도가 20℃∼65℃인 방법.
  17. 제15항에 있어서, 상기 온도가 30℃∼60℃인 방법.
  18. 페스탈로티오프시스 푸네레아 IFO 5427 (SANK 15174)의 단리 배양액.
  19. 페스탈로티오프시스 네글렉타 FERM BP-3501 (SANK 13390)의 단리 배양액.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920014523A 1991-08-13 1992-08-12 생 전분 소화 가능한 아밀라아제 KR930004461A (ko)

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JP91-202771 1991-08-13
JP20277191 1991-08-13

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Publication number Priority date Publication date Assignee Title
WO2003064617A2 (en) * 2002-01-30 2003-08-07 Montana State University Pestalotiopsis microsporia isolates and compounds derived therefrom
WO2011154529A1 (en) * 2010-06-11 2011-12-15 Novozymes A/S Enzymatic flour correction

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* Cited by examiner, † Cited by third party
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WO1983001458A1 (en) * 1981-10-26 1983-04-28 Lundell, Ralf Process for the preparation of enzymes
JPS59140896A (ja) * 1983-01-17 1984-08-13 Norin Suisansyo Shokuhin Sogo Kenkyusho カララ属のカビの生産する酵素を用いた澱粉の糖化法
EP0171218B1 (en) * 1984-08-06 1993-10-13 Genencor, Inc. Enzymatic hydrolysis of granular starch directly to glucose
JPS61141890A (ja) * 1984-12-15 1986-06-28 Suntory Ltd アルコ−ルの製造法
JPS62126989A (ja) * 1985-11-26 1987-06-09 Godo Shiyusei Kk コルテイシウム属担子菌の生産する酵素を用いた澱粉質の無蒸煮糖化法

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