KR900702371A - How to Detect Different Kinds of Antibodies and / or Antigens Simultaneously - Google Patents

How to Detect Different Kinds of Antibodies and / or Antigens Simultaneously

Info

Publication number
KR900702371A
KR900702371A KR1019900701302A KR900701302A KR900702371A KR 900702371 A KR900702371 A KR 900702371A KR 1019900701302 A KR1019900701302 A KR 1019900701302A KR 900701302 A KR900701302 A KR 900701302A KR 900702371 A KR900702371 A KR 900702371A
Authority
KR
South Korea
Prior art keywords
antibody
antigen
antibodies
solid carrier
detected
Prior art date
Application number
KR1019900701302A
Other languages
Korean (ko)
Inventor
안더쉬 발리네
세실 체르킨스키
Original Assignee
비로발 에스. 아.
안더쉬 발리네
세실 체르킨스키
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE8803689A external-priority patent/SE465943B/en
Application filed by 비로발 에스. 아., 안더쉬 발리네, 세실 체르킨스키 filed Critical 비로발 에스. 아.
Publication of KR900702371A publication Critical patent/KR900702371A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms

Abstract

내용 없음No content

Description

다른 종류의 항체 및 또는 항원을 동시에 검출하는 방법How to detect different kinds of antibodies and / or antigens simultaneously

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (10)

개개의 세포에 의해 분비 및/또는 배출되는 항체 및 또는 항원의 검출방법으로서, 셀 서프펜숀이 고체캐리어와 접촉되고, 그후 또는 그와 동시에 대향하고, 검출되는 항체 또는 항원에 결합하는 능력을 가진 다른 항체가 첨가된 후, 상기 다른 항체에는 문제의 항체 또는 항원을 검출하는 지시제 물질과 반응하는 효소를 공급하는 방법에 있어서, 적어도 두 종류의 상기 다른 항체가 검출되는 적어도 다른 두종류의 항체에 대향하여 첨가되고 상기 다른 항체에는 다른 효소가 공급되며, 그다음 다른 착색 효과를 갖는 해당수의 지시제 물질이 공급되어, 고체 캐리어에 남아있는 구별되는 색체의 스폿을 검사함으로서 다른 종류의 항체 및/또는 항원을 동시에 검출하는 것을 특징으로 하는 방법.A method of detecting antibodies and / or antigens secreted and / or excreted by individual cells, wherein the cell suspension is in contact with, then or simultaneously with, a solid carrier, and other with the ability to bind the antibody or antigen to be detected. After the antibody is added, the other antibody is supplied with an enzyme that reacts with an indicator substance for detecting the antibody or antigen in question, and at least two other types of antibodies for which at least two different antibodies are detected. Other antibodies and / or antigens, which are then added to the other antibody and then supplied with different enzymes, and then the corresponding number of indicator substances with different coloring effects, to examine spots of distinct chromosomes remaining in the solid carrier. Detecting at the same time. 제1항에 있어서, 상기 고체 캐리어는 여기에 결합되어 검출되는 항체 및/또는 항원에 대응하는 항원 및/ 또는 항체를 갖는 것을 특징으로 하는 방법.The method of claim 1, wherein the solid carrier has an antigen and / or antibody corresponding to and / or detected with the antibody bound thereto. 제2항에 있어서, 상기 고체 캐리어에 결합되어 있는 상기 항원 및/ 또는 항체는 검출될 다른 항체 및/또는 항원과의 반응력이 있는 하나의 단일 종류인 것을 특징으로 하는 방법.3. The method of claim 2, wherein said antigen and / or antibody bound to said solid carrier is one single type that is reactive with another antibody and / or antigen to be detected. 제2항에 있어서, 상기 고체 캐리어에 결합되어 있는 상기 항원 및/또는 항체는 검출될 다른 항체 및/ 또는 항원과의 반응력이 있는 다른 종류인 것을 특징으로 하는 방법.The method of claim 2, wherein the antigen and / or the antibody bound to the solid carrier is another kind to which the antigen and / or antigen is reactive. 제2항에 있어서, 상기 같거나 다른 종류와 상기 항원 및/또는 항체는 두 고체 캐리어의 두 반대표면에 결합되어, 셀서스펜숀이 두 캐리어 사이에 가해지는 것을 특징으로 하는 방법.The method of claim 2, wherein the same or different kind and the antigen and / or antibody are bound to two opposite surfaces of two solid carriers such that a cell suspension is applied between the two carriers. 제1항에 있어서, 상기 고체 캐리어는 내부 결합성질을 갖고 예로,니트로셀룰로우즈, 나일론 및 폴리비닐로 구성된 그룹에서 선택되는 물질인 것을 특징으로 하는 방법.The method of claim 1, wherein the solid carrier has an internal bond and is, for example, a material selected from the group consisting of nitrocellulose, nylon, and polyvinyl. 전술한 항들중 어느 한항에 있어서, 지시제 시스템이 알카리 포스파타제 및 고추냉이 과산화 효소 각각 및 대응하는 색소원 기질로 표지된 항체에 사용되는 것을 특징으로 하는 방법.The method according to any one of the preceding claims, wherein the indicator system is used for antibodies labeled with alkaline phosphatase and horseradish peroxidase, respectively, and with corresponding pigment source substrates. 제7항에 있어서, 상기 색소원 기질은 5-브로모-4-클로로-5-인돌일 포스페이트 롤루이딘 염 및 p-니트로블루 테트라졸리움 클로라이드(BCIP/NBT) 및 3-아미노-9-에틸-카르바졸(AEC)로 구성되는 것을 특징으로 하는 방법.The method of claim 7, wherein the pigment source substrate is 5-bromo-4-chloro-5-indolyl phosphate roluidine salt and p-nitroblue tetrazolium chloride (BCIP / NBT) and 3-amino-9-ethyl -Carbazole (AEC). 전술한 항들중 어느 한항에 있어서, 상기 검출되는 항체는 다른 종류의 Ig-항체를 포함하는 것을 특징으로 하는 방법.The method of any one of the preceding claims, wherein said antibody to be detected comprises a different class of Ig-antibody. 전술한 항들중 어느 한항에 있어서, 상기 검출되는 항체는 진균, 박테리아, 바이러스 및 기생충원의 생성물을 포함하는 것을 특징으로 하는 방법.The method of any one of the preceding claims, wherein said antibody to be detected comprises products of fungi, bacteria, viruses and parasites. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019900701302A 1988-10-14 1989-10-13 How to Detect Different Kinds of Antibodies and / or Antigens Simultaneously KR900702371A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
SE8803689-2 1988-10-14
SE8803689A SE465943B (en) 1988-10-14 1988-10-14 Method for simultaneous detection of different analytes, in particular antibodies, using the "ELISPOT" technique
US36737789A 1989-06-16 1989-06-16
US367,377 1989-06-16
PCT/SE1989/000565 WO1990004182A1 (en) 1988-10-14 1989-10-13 A method for simultaneous detection of different types of antibodies and/or antigens produced by individual cells

Publications (1)

Publication Number Publication Date
KR900702371A true KR900702371A (en) 1990-12-06

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KR1019900701302A KR900702371A (en) 1988-10-14 1989-10-13 How to Detect Different Kinds of Antibodies and / or Antigens Simultaneously

Country Status (4)

Country Link
EP (1) EP0438457A1 (en)
KR (1) KR900702371A (en)
AU (1) AU4346989A (en)
WO (1) WO1990004182A1 (en)

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GB8921880D0 (en) * 1989-09-28 1989-11-15 Medical Res Council Improvements in or relating to hybridomas
DE4211108A1 (en) * 1992-04-03 1993-10-07 Boehringer Mannheim Gmbh Virus solution for use in immunoassays
DE4232073C2 (en) * 1992-09-25 2001-09-20 Chiron Behring Gmbh & Co Method for the immunochemical quantification of inactivated, immunoreactive antigens
DE4236189A1 (en) * 1992-10-27 1994-04-28 Boehringer Mannheim Gmbh Method for the simultaneous determination of antigens and antibodies
US5750356A (en) * 1996-05-31 1998-05-12 Anergen, Inc. Method for monitoring T cell reactivity
DE19821289A1 (en) * 1998-05-13 1999-11-18 Volkmar Schoellhorn Method for determining the activity of human and animal cells, in particular blood cells, and microtiter plates
EP1081496A1 (en) * 1999-08-31 2001-03-07 Becton, Dickinson and Company Flow-through assay for visually detecting the presence of influenza A and B
DE10125730A1 (en) * 2001-05-17 2002-11-21 A I D Autoimmun Diagnostika Gm Detecting pathogens or cellular products induced by them, useful particularly for diagnosis of subacute spongiform encephalopathy
GB0409775D0 (en) 2004-04-30 2004-06-09 Mabtech Ab Assay
GB0409771D0 (en) * 2004-04-30 2004-06-09 Mabtech Ab Assay
WO2009035738A2 (en) * 2007-06-15 2009-03-19 University Of Rochester Use of antibody secreting cell elispot to assess antibody responses following antigen exposure
DE102007052517A1 (en) 2007-10-29 2009-04-30 Autoimmun Diagnostika Gmbh ELISPOT process with two filter systems
GB0815675D0 (en) 2008-08-28 2008-10-08 Mabtech Ab Antibody secreting cell elispot

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Publication number Priority date Publication date Assignee Title
CA1148859A (en) * 1979-06-14 1983-06-28 Lacy R. Overby Simultaneous assay of two hepatitis viruses using a solid phase
US4690890A (en) * 1984-04-04 1987-09-01 Cetus Corporation Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay
GB8607101D0 (en) * 1986-03-21 1986-04-30 Serono Diagnostics Ltd Immunoassay
AU1295188A (en) * 1987-01-20 1988-08-10 Interferon Sciences Inc. Multicolor western blot

Also Published As

Publication number Publication date
WO1990004182A1 (en) 1990-04-19
EP0438457A1 (en) 1991-07-31
AU4346989A (en) 1990-05-01

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