KR900001514B1 - Method for fermentation - Google Patents

Method for fermentation Download PDF

Info

Publication number
KR900001514B1
KR900001514B1 KR1019870005448A KR870005448A KR900001514B1 KR 900001514 B1 KR900001514 B1 KR 900001514B1 KR 1019870005448 A KR1019870005448 A KR 1019870005448A KR 870005448 A KR870005448 A KR 870005448A KR 900001514 B1 KR900001514 B1 KR 900001514B1
Authority
KR
South Korea
Prior art keywords
fermentation
porous
gas
bod
cod
Prior art date
Application number
KR1019870005448A
Other languages
Korean (ko)
Other versions
KR880014101A (en
Inventor
홍종준
Original Assignee
재단법인 한국동력자원연구소
박공식
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 재단법인 한국동력자원연구소, 박공식 filed Critical 재단법인 한국동력자원연구소
Priority to KR1019870005448A priority Critical patent/KR900001514B1/en
Publication of KR880014101A publication Critical patent/KR880014101A/en
Application granted granted Critical
Publication of KR900001514B1 publication Critical patent/KR900001514B1/en

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84—Biological processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Processing Of Solid Wastes (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Various organic compounds convert into inflammable gas by fermentation. Thus, 1400ml of organic compounds containing TS 2.011g/100cc and VS 1.661g/100cc (COD 4213 mg/l; BOD 3890mg/l; ph 6.20) are placed in 2000ml digestive vessel, mixed with 280ml of digestive compounds containing TS 2.18g/100cc and VS 1.80g/100cc (COD 8130mg/l; BOD 7210mg/l; ph 7.30), and fermented anaerobically at 35oC. The anaerobes used in the fermentation are immobilized in a poly- porous phenol resin plate.

Description

다공성 미생물 고정막에 의한 속성 발효방법Rapid Fermentation Method by Porous Microbial Membrane

제1도는 혐기성 소화액으로부터 발생되는 가스 조성을 나타낸 가스 크로마토그램.1 is a gas chromatogram showing the gas composition generated from the anaerobic digestion liquid.

제2도는 본 발명에 의한 미생물 고정판을 소화조 장치 내부에 설치한 상태를 나타낸 종단 예시도.Figure 2 is an exemplary view showing a state in which the microbial fixing plate according to the present invention installed in the digester apparatus.

제3도는 본 발명의 실시예 1의 조건에 따라 발효된 소화종(消化種)에서 생성된 가스 조성 및 가스 발생량 측정도.3 is a gas composition and gas generation amount measured in the digested species fermented according to the conditions of Example 1 of the present invention.

제4도는 동 실시예 2의 조건에 따라 발효된 소화종에서 생성된 가스 조성 및 가스 발생량 측정도.4 is a gas composition and gas generation amount measurement generated from the digested species fermented according to the conditions of Example 2.

본 발명은 속성 발효법에 관한 것이다. 좀 더 구체적으로 설명하면, 각종 분해성 유기물질들을 가연성 가스로 전환시킴에 있어, 순도가 높고 생산 수율이 향상된 가스를 회수하는, 다공성 미생물 고정막에 의한 발효 방법에 관한 것이다.The present invention relates to a fast fermentation method. More specifically, the present invention relates to a fermentation method using a porous microbial fixation membrane, which recovers a gas having high purity and improved production yield in converting various degradable organic substances into flammable gas.

종래의 2상 발효방법 또는 발효조 내부에 충전물을 투입하여 미생물의 농도를 상승시키는 방법들에 있어서는, 대체로 당해 기술들에 있어서는 만족하기는 하지만, 본 발명에서는 응용할 수가 없다.In the conventional two-phase fermentation method or the method of raising the concentration of microorganisms by adding a filler into the fermenter, although generally satisfactory in the techniques, it is not applicable in the present invention.

2상 발효법의 경우, 시설비가 막대할 뿐 아니라 운전, 관리에 많은 애로점에 있고 충진 방법에 있어서는 충진물의 량에 비교하여 표면적이 증대하지 않기 때문에 비경제적임은 물론, 충진재에 있어서도, 그의 재질 및 구성 방법에 관하여 기술적인 해결을 보디 못하고 있는 실정에 있다.In the case of the two-phase fermentation method, not only is it expensive, but also has many difficulties in operation and management, and the filling method is uneconomical because the surface area does not increase compared to the amount of the filling material, and also in the filling material, its material and composition method There is no technical solution to the situation.

본 발명은 이들 공지 기술과는 전연 달리, 소화조 내부에 단위 체적당 미생물의 농도를 상승시키기 위하여 다공성 페놀 발포 수지판을 만들어, 발포 수지막 표면을 무기 영양재로 처리한 다음, 여기에 미생물 부착용 피막을 형성하여 미생물을 고정시킨 다공성 미생물 고정판을 소화조 내부에 고정하여 속성 발효케 하므로서, 순도가 높고 생산 수율이 향상된 가스를 회수할 수 있게 함을 특징으로 한 것이다.Unlike the prior art, the present invention makes a porous phenolic foamed resin plate to increase the concentration of microorganisms per unit volume in the digester, and treats the surface of the foamed resin film with an inorganic nutrient, and then attaches the film for attaching the microorganism thereto. Forming a porous microbial fixing plate fixed to the microorganisms to fix the inside of the digester to accelerate the fermentation, it characterized in that it is possible to recover the gas with high purity and improved production yield.

이와 같은 본 발명을 구체적으로 설명하면 다음과 같다.The present invention will be described in detail as follows.

① 다공성 페놀 수지용 농축수지의 합성① Synthesis of concentrated resin for porous phenolic resin

페놀(Phenol), 크레졸(Cresol), 크실레놀(Xylenol)류의 100g에 대해 파라포름알데히드(Paraformaldehyde)를 1.2∼2.0mol되게 하여 수산화바륨(Barium hydroxide) 102g을 가하고 냉각기 및 냉각쟈켓(Jaket)이 있는 반응기 내에서 90∼110℃에서 60∼120분간 반응시켜 분자량 350∼500 범위로 한 다음, 수산, 파라톨루엔술폰산(Para-Toluens Sulfonic acid) 및 크실렌 술폰산(Xylene Sulfonic acid), 등의 유기산을 0.002∼0.02mol 투입하여 pH 6∼6.5 되게 중화후 수지분이 80∼85% 되게 감압 탈수한 농축 수지를 발포용 페놀수지로 한다.To 100 g of phenol, cresol, and xylenol, add 1.2 to 2.0 mol of paraformaldehyde, add 102 g of barium hydroxide, and cooler and jacket. In a reactor containing 90 to 110 ° C. for 60 to 120 minutes to obtain a molecular weight of 350 to 500, followed by organic acids such as hydroxyl, para-toluens sulfonic acid and xylene sulfonic acid. The concentrated resin dehydrated under reduced pressure to 80-85% after neutralization by adding 0.002-0.02mol to pH 6-6.5 is used as foaming phenol resin.

② 다공성 페놀 수지판② porous phenolic resin plate

감압 탈수한 페놀-포름알데히드 합성 농축 수지 100g에 대해 발포재인 디니트로펜타메치렌테트라민, 중탄산나트륨 및 중탄산칼슘 0.5g와 분산재인 에마논 3113(Emanone 3113) 및 알킬 아릴 술폰산(Alkyl aryl sulfonic acid) 0.2g와 경화재인 핵사메틸렌테트라민(Hexamethylene tetramine), 파라톨루엔술폰산(Para toluene sulfonic acid) 및 인산 0.3g와 발포조재인 프탈산(Phthalic acid) 및 벤토나이트(Bentonite) 0.15g를 40∼ 45℃ 상태에서 급속히 혼합하여 40∼45℃로 유지된 발포 성형틀에 넣어 3∼5m/m 기공의 다공성 페놀 수지판이 되게 발포한다.To 100 g of dehydrated phenol-formaldehyde synthetic concentrated resin, 0.5 g of dinitropentamethylenetetramine, sodium bicarbonate, calcium bicarbonate, and dispersant Emanone 3113 and alkyl aryl sulfonic acid 0.2 g and a hardener, hexamethylene tetramine, para toluene sulfonic acid and 0.3 g of phosphoric acid, and 0.15 g of foaming aid, phthalic acid and bentonite, were rapidly treated at 40 to 45 ° C. The mixture is mixed into a foam molding mold maintained at 40 to 45 ° C. and foamed to form a porous phenol resin plate having 3 to 5 m / m pores.

③ 미생물 고정막 형성③ Microbial fixed membrane formation

다공성 페놀수지 발포에 잔존하는 유리산을 알칼리 용액으로 중화하여 충분히 세척한 다음 건조하여 미생물 무기염 배지액인 K2PHO4, 0.2∼0.25%; (NH4)2SO4, 0.25∼0.3%; MgSO4·7H2O, 0.005∼0.006%; NH2CONH2, 0.08∼0.1%; 의 혼합용액에 침적하여 기공 표면 처리를 한 다음 혐기성 미생물의 표면 부착을 용이하게 하기 위하여 배지액 표면을 한천(Agar) 1.2∼1.5% 용액으로 피막을 형성시킨다.Neutralizing the free acid remaining in the foaming of the porous phenolic resin with an alkaline solution, sufficiently washing and drying, followed by drying of K 2 PHO 4 , 0.2 to 0.25%, which is a microbial inorganic salt medium solution; (NH 4 ) 2 SO 4 , 0.25-0.3%; MgSO 4 7H 2 O, 0.005 to 0.006%; NH 2 CONH 2 , 0.08-0.1%; After pore surface treatment by immersing in the mixed solution of to form a film with agar (Agar) 1.2-1.5% solution in order to facilitate the surface adhesion of anaerobic microorganisms.

④ 다공성 미생물 고정막 발포판의 혐기성 미생물 고정④ Anaerobic Microbial Fixation of Porous Microbial Fixation Membrane Foam Plate

미리 혐기성 배양된 소화조 내부 접종액에 필름 형성 고정막을 주입하여 혐기교반 조건하에서 30∼35℃의 중온으로 미생물을 발포한 표면에 고착시킨다.A film-forming fixed membrane is injected into the inoculum incubated in an anaerobic culture in advance and adhered to the surface on which the microorganisms are foamed at a medium temperature of 30 to 35 ° C. under anaerobic stirring conditions.

위와 같은 본 발명에 의한, 혐기성 소화액으로부터 발생되는 가스 조성은 도면 제1도와 같은 조건의 소화액에 침적한 미생물 고정판을 도면 제2도에 도시된 방법으로 소화조 장치 내부에 설치하여 연속적인 유기 물질을 투입하면서 소화시킨 결과, 다공성 미생물 고정판을 투입한 발효장치는 투입하지 않은 일반 장치에 비교하여 동일 조건하에서 소화 능력이 평균 40% 향상되었으며 또한 발생가스 중 메탄 함량도 7% 향상되어 결과적으로 열량으로 60%가 향상되어짐을 알게 되었다.According to the present invention, the gas composition generated from the anaerobic digestion liquid is a microorganism fixing plate deposited in the digestion liquid under the conditions shown in FIG. 1 is installed in the digester apparatus by the method shown in FIG. As a result of digestion, the fermentation system with a porous microorganism fixed plate improved the digestion capacity by 40% on average under the same conditions and the methane content in the generated gas by 7%, resulting in 60% heat. It has been found that is improved.

[실시예 1]Example 1

유기물 조성이 TS 2.011g/100cc, VS 1.661g/100cc, COD 4,213mg/1, BOD 3890mg/1, pH 6.20인 용액을 발효하기 위하여 2,000ml 용량의 소화조 내에 1400ml을 주입후 소화종(消化種)조성이 2.18g/100cc, VS 1.80g/100cc, COD 8,130mg/1, BOD 7,210mg/1, pH 7.30 인 용액 280ml를 가하여 35℃의 온도를 유지시키면서 혐기성 교반 조건하에서 발효하였을 시 시간의 경과에 따라 생성되는 가스 조성 및 가스량을 각각 가스 크로마토그라프 및 적산 유량계로서 측정하면서 정상적인 가스 조성점인 메탄(CH4) 함량이 65% 지점에 이르렀을 때 유기물 부하는 메탄 함량이 약 60% 유지되는 조건에서 유기물을 계속 주입하면서 측정하였다. 그 결과는 도면 제3도와 같다.Digestion after injecting 1400ml into 2,000ml digester to ferment solution with TS 2.011g / 100cc, VS 1.661g / 100cc, COD 4,213mg / 1, BOD 3890mg / 1, pH 6.20 280 ml of a solution with a composition of 2.18 g / 100 cc, VS 1.80 g / 100 cc, COD 8,130 mg / 1, BOD 7,210 mg / 1, pH 7.30 was added and the fermentation was carried out under anaerobic stirring conditions while maintaining the temperature at 35 ° C. According to the gas chromatograph and cumulative flow meter, respectively, the gas composition and the amount of gas produced are measured, and when the methane (CH 4 ) content, which is a normal gas composition point, reaches 65%, the organic load is maintained at about 60%. Measurement was made while continuing to inject the organics. The result is shown in FIG. 3.

[실시예 2]Example 2

2,000ml 의 소화조 내에 250ml/V 용적의 다공성 고정판을 훈양(馴養)된 소화종(消化種)용액속에 넣어 35℃온도를 유지하면서 혐기 조건하에서 40일간 균을 고정화한 미생물막판을 설치하여 유기물 조성이 TS 2.011g/100cc, VS 1.661g/100cc, COD 4,213mg/1, BOD 3,890mg/1, pH 6.20인 용액 1,400ml를 주입 후 실시예1의 소화종(消化種)용액을 280ml 가하여 35℃의 온도를 유지시키면서 혐기성 교반 조건하에 발효 시간의 경과에 따라 실시예1과 동일한 방법으로 분석 측정하였다.In a 2,000 ml digester, a 250 ml / V volume porous fixation plate was placed in a cultivated digestive species solution and a microbial membrane plate was immobilized for 40 days under anaerobic conditions while maintaining a temperature of 35 ° C. After injection of 1,400 ml of TS 2.011g / 100cc, VS 1.661g / 100cc, COD 4,213mg / 1, BOD 3,890mg / 1, pH 6.20, 280ml of the digestive species solution of Example 1 was added to Analytical measurements were carried out in the same manner as in Example 1 with the passage of the fermentation time under anaerobic stirring conditions while maintaining the temperature.

그 결과는 도면 제4도와 같다.The result is shown in FIG. 4.

Claims (1)

분해성 유기물질을 속성 발효함에 있어서, 다공성 발포수지판을 이용한 미생물 고정판을 소화조 내부에 설치하여 발효시킴을 특징으로 하는 다공성 미생물 고정막에 의한 속성 발효 방법.In the fast fermentation of the degradable organic material, the fast fermentation method by a porous microorganism fixed membrane, characterized in that the fermentation by installing a microbial fixing plate using a porous foam resin plate in the digester.
KR1019870005448A 1987-05-30 1987-05-30 Method for fermentation KR900001514B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019870005448A KR900001514B1 (en) 1987-05-30 1987-05-30 Method for fermentation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019870005448A KR900001514B1 (en) 1987-05-30 1987-05-30 Method for fermentation

Publications (2)

Publication Number Publication Date
KR880014101A KR880014101A (en) 1988-12-22
KR900001514B1 true KR900001514B1 (en) 1990-03-12

Family

ID=19261797

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019870005448A KR900001514B1 (en) 1987-05-30 1987-05-30 Method for fermentation

Country Status (1)

Country Link
KR (1) KR900001514B1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7589654B2 (en) 2007-09-05 2009-09-15 Hynix Semiconductor Inc. Digital-to-analog converting circuit and apparatus for on-die termination using the same
US7602661B2 (en) 2006-08-16 2009-10-13 Hynix Semiconductor Inc. Semiconductor memory apparatus and method of controlling the same

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7602661B2 (en) 2006-08-16 2009-10-13 Hynix Semiconductor Inc. Semiconductor memory apparatus and method of controlling the same
US7589654B2 (en) 2007-09-05 2009-09-15 Hynix Semiconductor Inc. Digital-to-analog converting circuit and apparatus for on-die termination using the same

Also Published As

Publication number Publication date
KR880014101A (en) 1988-12-22

Similar Documents

Publication Publication Date Title
US10633622B2 (en) Integrated system for hydrogen and methane production from industrial organic wastes and biomass
Hanaki et al. Protection of methanogenic bacteria from low pH and toxic materials by immobilization using polyvinyl alcohol
Wiegant et al. Separation of the propionate degradation to improve the efficiency of thermophilic anaerobic treatment of acidified wastewaters
Murray et al. Effect of support material on the development of microbial fixed films converting acetic acid to methane
van Lier et al. Start-up of a thermophilic upflow anaerobic sludge bed (UASB) reactor with mesophilic granular sludge
Smith et al. Kinetics of acetate metabolism during sludge digestion
Lin et al. Hydrogen production from sucrose using an anaerobic sequencing batch reactor process
Kennedy et al. Stability and performance of anaerobic fixed film reactors during hydraulic overloading at 10–35° C
Karube et al. Methane production from wastewaters by immobilized methanogenic bacteria
KR900001514B1 (en) Method for fermentation
EP0604708A1 (en) Fermentation method for the fast production of methane
CN113755531A (en) Method for promoting cow dung straw anaerobic co-fermentation
Hattingh et al. Biological changes during the adaptation of an anaerobic digester to a synthetic substrate
CN112390359B (en) Preparation and application method of filler with enhanced methane production function
Wu et al. Metabolic properties and kinetics of methanogenic granules
Ahmed et al. Growth rate dependence of solventogenesis and solvents produced by Clostridium beijerinckii
Fox et al. Anaerobic treatment of high-sulfate wastewater and substrate interactions with isopropanol
Joubert et al. The effect of pH and temperature manipulation on metabolite composition during acidogenesis in a hybrid anaerobic digester
CN110627183A (en) Preparation method of slow-release composite oxygen release material
Smiti et al. Thermophilic degradation of cellulose by a triculture of Clostridium thermocellum, Methanobacterium sp. and Methanosarcina MP
Gas Biogas
Madamwar et al. Effect of mixture of surfactants and adsorbents on anaerobic digestion of water hyacinth-cattle dung
Miyahara et al. Behavior of suspended solids and anaerobic bacteria in an anaerobic fixed bed reactor
SU1104152A1 (en) Syntrophic association of methanosarcina majei,methanothrix soehugenii,methanobacterium formicicum no 1002 microorganisms for processing cattle manure to methan
CN112661376A (en) Municipal sludge pretreatment method and application thereof

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
G160 Decision to publish patent application
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20060215

Year of fee payment: 17

EXPY Expiration of term