KR880004093A - Cloning of genes involved in biotin biosynthetic chain reaction and methods of producing biotin - Google Patents

Cloning of genes involved in biotin biosynthetic chain reaction and methods of producing biotin Download PDF

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KR880004093A
KR880004093A KR870011002A KR870011002A KR880004093A KR 880004093 A KR880004093 A KR 880004093A KR 870011002 A KR870011002 A KR 870011002A KR 870011002 A KR870011002 A KR 870011002A KR 880004093 A KR880004093 A KR 880004093A
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biotin
dna sequence
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글로클레 레미
스페끄 데니스
레모인느 이브
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에띠엔느 에장만느
트랑스젠느 에스.아.
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Abstract

내용 없음No content

Description

비오틴 생합성 연쇄반응에 관련되어 있는 유전자들의 클로닝과 비오틴의 제조 방법Cloning of genes involved in biotin biosynthetic chain reaction and methods of producing biotin

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 비오틴 생합성 연쇄반응.1 is a biotin biosynthetic chain reaction.

제2도는 플라스미드 pTG1400 개요도.2 is a schematic representation of plasmid pTG1400.

제3도는 플라스미드 pTG475 개요도.3 is a schematic representation of plasmid pTG475.

Claims (31)

박테리아에서의 비오틴 생합성 연쇄반응에 관련되어 있는 bioA, bioD, bioF, bioC 및 bioH 유전자들중 하나에 의해 생성되는 효소를 코딩하는 DNA 시퀀스.DNA sequence encoding an enzyme produced by one of the bioA, bioD, bioF, bioC and bioH genes involved in biotin biosynthetic chain reaction in bacteria. 제1항에 있어서, bioB와 bioD유전자들에 의해 생성되는 효소를 코딩하는 DNA 시퀀스.The DNA sequence of claim 1, encoding an enzyme produced by bioB and bioD genes. 제1항에 있어서, bioD와 bioA 및 bioB 유전자들에 의해 생성되는 효소를 코딩하는 DNA 시퀀스.The DNA sequence of claim 1, encoding an enzyme produced by bioD and bioA and bioB genes. 제1항에 있어서, bioD와 bioA, bioB 및 bioF 유저자들에 의해 생성되는 효소를 코딩하는 DNA 시퀀스.The DNA sequence of claim 1, encoding an enzyme produced by bioD and bioA, bioB, and bioF users. 박테리아에서의 비오틴 생합성 연쇄반응에 관련되어 있는 bioF와 bioC, bioF와 bioH 및 bioF와 bioC 및 bioH 유전자들중 하나에 의해 생성되는 효소를 코딩하는 DNA 시퀀스.DNA sequence encoding an enzyme produced by one of the bioF and bioC, bioF and bioH and bioF and bioC and bioH genes involved in biotin biosynthetic chain reaction in bacteria. 제5항에 있어서, 상기외에 bioA 또는 bioA 와 bioD 유전자에 의해 생성되는 적어도 하나의 효소를 코딩하는 DNA 시퀀스.The DNA sequence of claim 5, wherein said DNA encodes at least one enzyme produced by said bioA or bioA and bioD genes. 제5항 또는 제6항에 있어서, bioD와 bioA 및 bioB 유전자들에 의해 생성되는 효소들을 코딩하는 DNA 시퀀스.The DNA sequence according to claim 5 or 6, which encodes enzymes produced by bioD and bioA and bioB genes. 제1항 내지 제6항중 어느 하나의 항에 있어서, 근원박테리아에서 비오틴 생합성경로상의 효소들의 전사를 조절해주는 천연의 시퀀스가 없는 DNA 스퀀스.The DNA sequence of claim 1, wherein the DNA sequence lacks a native sequence that regulates the transcription of enzymes on the biotin biosynthetic pathway in the myobacteria. 제1항 내지 제6항중 어느 하나의 항에 있어서, 제4도 내지 제8도와 제17도 내지 제19도에 표시된 시퀀스의 전부 또는 일부에 해당하는 DNA 시퀀스.The DNA sequence according to any one of claims 1 to 6, which corresponds to all or part of the sequence shown in FIGS. 4 to 8 and 17 to 19. 제1항 내지 제6항중 어느하나의 항에 있어서, 간균(Bacillus)의 균주로부터 발생되는 DNA 시퀀스.The DNA sequence according to any one of claims 1 to 6, which is generated from a strain of Bacillus. 제10항에 있어서, B.sphaericus의 균주로부터 발생되는 DNA 시퀀스.The DNA sequence of claim 10 which is generated from a strain of B.sphaericus. 제1항 내지 제11항중 어느하나의 항에 따른 적어도 하나의 시퀀스를 함유하는 플라스미드 벡터.A plasmid vector containing at least one sequence according to any one of claims 1 to 11. 제12항에 있어서, 숙주세포내의 염색체내로 통합될 수 있는 DNA 시퀀스를 함유하는 벡터.The vector of claim 12, wherein the vector contains a DNA sequence capable of incorporation into a chromosome in a host cell. 제13항에 있어서, 형질변환되는 균주의 게놈(genome)에 존재하는 시퀀스와 동질인 통합을 위해 제공되는 적어도 하나의 시퀀스를 함유하는 벡터.The vector of claim 13, wherein the vector contains at least one sequence provided for integration homogeneous with a sequence present in the genome of the strain to be transformed. 제14항에 있어서, 동질의 시퀀스는 천연의 게놈시퀀스인 벡터.The vector of claim 14, wherein the homologous sequence is a native genome sequence. 제14항에 있어서, 동질의 시퀀스는 플라스미드 통합벡터에 의해 게놈내에 통합되어진 시퀀스인 벡터.The vector of claim 14, wherein the homogeneous sequence is a sequence integrated into the genome by a plasmid integration vector. 제16항에 있어서, 특정 틀라스미드 벡터는 변형되는 균주의 촉진인자 조절하에서 항생물질과 마커(marker) 유전자에 저항하는 적어도 하나의 유전자를 함유하는 벡터.17. The vector of claim 16, wherein the particular tlasmid vector contains at least one gene that resists antibiotics and marker genes under promoter control of the strain being modified. 제17항에 있어서, 촉진인자가 유도가능한 촉진인자인 벡터.The vector of claim 17, wherein the promoter is an inducible promoter. 제18항에 있어서, 플라스미드 통합 벡터는 플라스미드 pTG475인 벡터.The vector of claim 18, wherein the plasmid integration vector is plasmid pTG475. 제12항에 있어서, 자동의 복제근원(origin)를 함유하는 벡터.13. The vector according to claim 12, which contains an automatic origin of replication. 제20항에 있어서, 문제의 근원이 간균의 균주나 에스케리히아(Escherichia)균주에서의 자동 복제근원인 벡터.21. The vector of claim 20, wherein the source of the problem is a source of autonomous replication in strains of E. coli or Escherichia strains. 제20항 또는 제21항에 있어서, 비오틴 생합성의 연쇄반응에 관련되어 있는 효소들을 코딩하는 DNA 시퀀들은 숙주에서 이들이 발현되도록 하는 인자들에 의해 인접되어있는 벡터.The vector of claim 20 or 21, wherein the DNA sequences encoding enzymes involved in the biotin biosynthesis chain reaction are contiguous by factors that allow them to be expressed in the host. 제22항에 있어서, 숙주에서 유효한 촉진인자와 종료인자인 그의 발현을 위해 제공하는 인자들사이에서 나오게 되는 벡터.23. The vector of claim 22, wherein the vector comes between those factors that provide for their expression as effective promoters and terminators in the host. 제12항 내지 제23항중 어느하나의 항에 따른 벡터로 형질변환된 세포.A cell transformed with the vector according to any one of claims 12 to 23. 제24항에 있어서, 간균속, 에스케리히아속 또는 슈도모나스속으로 부터 선택된 박테리아인 세포.The cell of claim 24 which is a bacterium selected from the genus Bacillus, Escherichia or Pseudomonas. 제24항에 있어서, 효모균속의 효모인 세포.25. The cell of claim 24 which is a yeast of the genus Yeast. 제25항에 있어서, 박테리아는 B.sphaericus와 B.subtilis 및 E.coli 중에서 선택되는 세포.The cell of claim 25, wherein the bacterium is selected from B. sphaericus and B. subtilis and E. coli. 제24항 내지 제27항중 어느 하나의 항에 있어서, 형질변환세포는 비오틴이나 그의 비타머(vitamer)중 하나를 생성하는 세포인 것을 특징으로 하는 세포.28. The cell of any one of claims 24 to 27, wherein the transformed cell is a cell that produces either biotin or one of its vitamins. 적어도 피멜린산이나 비오틴의 비타머중 하나를 함유하는 배양매체를 피멜린산이나 비오틴의 비타머에 투과할 수 있는 제24항 내지 제28항중 어느하나의 항에 따른 세포롤 발효시키고 형성된 비오틴을 회수하여 비오틴을 제조하는 방법.A biotin formed by fermenting a cell roll according to any one of claims 24 to 28 which can permeate at least one of pimeline acid or biotin betamer to the betamer or biotin betamer. Recovery to produce biotin. 제29항에 있어서, 비오틴의 비타머는 적어도 피멜린산을 함유한 배양매체를 제24항 내지 제28항중 어느 하나의 항에 따른 세포로 발효시켜서 얻어짐을 특징으로 하는 방법.30. The method of claim 29, wherein the biotin biotamer is obtained by fermenting a culture medium containing at least pimelic acid with the cells according to any one of claims 24 to 28. 제30항에 있어서, 하나는 피멜린산으로부터 비오틴의 비타머을 생산하고, 다른 것은 이 비타머를 발효하여 비오틴을 생산하는, 제24항 내지 제28항중 어느하나에 따른 2개의 세포균주를 사용하여서 공발효시킴을 특징으로 하는 방법.31. The use of two cell strains according to any one of claims 24 to 28, wherein one produces a biotin biotin from pimeline and the other ferments the biotamer to produce biotin. A method characterized by co-fermentation. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR870011002A 1986-09-30 1987-09-30 Cloning of genes involved in biotin biosynthetic chain reaction and methods of producing biotin KR880004093A (en)

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FR8613603 1986-09-30
FR8613603A FR2604436B1 (en) 1986-09-30 1986-09-30 CLONING OF BIOA, BIOD, BACILLUS SPHAERICUS BIOF, TRANSFORMED VECTORS AND CELLS AND PROCESS FOR THE PREPARATION OF BIOTIN

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JPS61149091A (en) * 1984-12-24 1986-07-07 Nippon Soda Co Ltd Duplex dna to code biotin synthase, bacterium containing same and production of biotin
NZ217336A (en) * 1985-08-26 1988-06-30 Amgen Production of biotin from microorganisms

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