KR20240099957A - Feed additive composition for improving protein utilization and reducing greenhouse gas emissions - Google Patents
Feed additive composition for improving protein utilization and reducing greenhouse gas emissions Download PDFInfo
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- KR20240099957A KR20240099957A KR1020220181944A KR20220181944A KR20240099957A KR 20240099957 A KR20240099957 A KR 20240099957A KR 1020220181944 A KR1020220181944 A KR 1020220181944A KR 20220181944 A KR20220181944 A KR 20220181944A KR 20240099957 A KR20240099957 A KR 20240099957A
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- feed
- greenhouse gas
- gas emissions
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- reducing greenhouse
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- 229940055695 pancreatin Drugs 0.000 description 1
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- 230000001105 regulatory effect Effects 0.000 description 1
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- 229920005989 resin Polymers 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
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- 150000003839 salts Chemical class 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
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- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Fodder In General (AREA)
Abstract
본 발명은 단위동물(monogastric animal)에서 단백질과 아미노산의 이용률을 증진시키고 온실가스 배출을 저감시킬 수 있는 사료첨가제 조성물, 배합사료 조성물 및 이를 이용한 분뇨로부터 발생하는 아산화질소 배출을 저감시키는 방법에 관한 것이다. 본 발명에 따른 사료첨가제 조성물은 단위동물(monogastric animal)에 적용하는 경우 단위동물의 단백질과 아미노산 이용률을 효과적으로 증진시키고, 단위동물의 분뇨로부터 발생하는 아산화질소 배출을 저감시키는 효과가 우수한바, 단백질 이용률 증진 및 온실가스 배출 저감을 위한 사료첨가제 조성물로서 유용하게 사용될 수 있다.The present invention relates to a feed additive composition that can improve the utilization of proteins and amino acids in monogastric animals and reduce greenhouse gas emissions, a compound feed composition, and a method for reducing nitrous oxide emissions generated from manure using the same. . When applied to monogastric animals, the feed additive composition according to the present invention effectively improves the protein and amino acid utilization of monogastric animals and is excellent in reducing nitrous oxide emissions from monogastric animals' excrement, thereby increasing protein utilization. It can be usefully used as a feed additive composition for improving and reducing greenhouse gas emissions.
Description
본 발명은 단위동물(monogastric animal)에서 단백질과 아미노산의 이용률을 증진시키고 온실가스 배출을 저감시킬 수 있는 사료첨가제 조성물, 배합사료 조성물 및 이를 이용한 분뇨로부터 발생하는 아산화질소 배출을 저감시키는 방법에 관한 것이다.The present invention relates to a feed additive composition that can improve the utilization of proteins and amino acids in monogastric animals and reduce greenhouse gas emissions, a compound feed composition, and a method for reducing nitrous oxide emissions generated from manure using the same. .
양돈 사료 내 높은 함량의 조단백질 사용은 생산성 측면에서 긍정적인 효과를 보이나, 질소배출, 악취발생, 및 온실가스 등 환경적 측면의 문제로 국가적으로 사용 함량을 규제하고 있다.The use of high amounts of crude protein in pig feed has a positive effect in terms of productivity, but the amount used is nationally regulated due to environmental issues such as nitrogen emissions, odor generation, and greenhouse gases.
농림축산식품부(장관 김현수, 이하 농식품부)는 축산분야 온실가스 및 악취저감, 가금사료의 메치오닌 성분의 성분등록 방법 개선 등 「사료관리법」에 따른 「사료 등의 기준 및 규격」(농림축산식품부 고시 제2021-99)을 2021년 12월 29일 개정·시행한다고 밝혔다. 개정안은 양돈용 배합사료 내 조단백질 함량의 허용기준을 성장단계별로 14~23%까지 제한하던 것을 13~20%로 1~3%p 낮췄으며, 양돈용 배합사료와 달리 조단백질 함량을 제한하지 않았던 축우용(고기소, 젖소) 및 가금용(닭, 오리) 배합사료에 대해서도 축종별·성장단계별로 조단백질의 허용기준을 15~24%로 신설하였다(지속가능한 축산을 위한 사료공정서 개정, 2022.01.09 11:00:00 축산정책국 축산환경자원과 보도자료 참조).The Ministry of Agriculture, Food and Rural Affairs (Minister Kim Hyun-soo, hereinafter referred to as the Ministry of Agriculture, Food and Rural Affairs) has established the “Standards and Specifications for Feed, etc.” (Notice of the Ministry of Agriculture, Food and Rural Affairs) in accordance with the “Feed Management Act,” including reducing greenhouse gases and odors in the livestock sector and improving the registration method for methionine in poultry feed. It was announced that Article 2021-99) will be revised and implemented on December 29, 2021. The amendment lowered the allowable standard for crude protein content in mixed feed for pig farming by 1 to 3 percentage points from 14 to 23% by growth stage to 13 to 20%, and, unlike mixed feed for pig farming, did not limit the crude protein content for cattle ( For mixed feed for meat cattle, dairy cattle) and poultry (chicken, duck), the allowable crude protein standard of 15-24% has been established by livestock species and growth stage (Revised Feed Process for Sustainable Livestock Farming, 2022.01.09 11: 00:00 Livestock Policy Bureau, Livestock Environment and Resources Division (see press release).
조단백질 함량을 줄이고 생산성을 유지하기 위해서는 고가의 단백질 원료 사용 또는 고가의 아미노산을 추가적으로 첨가해야하나 생산성 유지를 위한 사료 가격 상승은 농가의 경제적 부담으로 작용하고 있다. 이에, 질소 이용률을 증가시키고 경제적 부담을 줄이기 위한 방안이 필요하다.In order to reduce the crude protein content and maintain productivity, it is necessary to use expensive protein raw materials or add expensive amino acids, but the increase in feed prices to maintain productivity is acting as an economic burden on farms. Accordingly, measures are needed to increase nitrogen utilization and reduce economic burden.
이에 본 발명자는 단위동물(monogastric animal)에서 단백질과 아미노산의 이용률을 증진시키고 온실가스 배출을 저감시킬 수 있는 사료첨가제를 발명하고자 노력하였으며, 그 결과 사료첨가제로 프로테아제 및 베타인 염산염을 이용하는 경우 가축의 단백질 이용률이 증진되고, 분뇨에서 온실가스 배출이 저감됨을 확인하였다.Accordingly, the present inventor tried to invent a feed additive that could improve the utilization of proteins and amino acids in monogastric animals and reduce greenhouse gas emissions. As a result, when protease and betaine hydrochloride are used as feed additives, the It was confirmed that protein utilization was improved and greenhouse gas emissions from manure were reduced.
자세하게는, 돼지 체외 위·장관 소화모델을 설계하고 이를 이용한 실험관 내(In vitro) 아미노산 소화율 측정하였으며, 그 결과 사료첨가제로 프로테아제 및 베타인 염산염을 첨가한 처리구에서 다른 처리구 대비 유의적으로 높은 (P < 0.05) 단백질 및 아미노산 소화율을 보여주는 것을 확인하였다. 뿐만 아니라, 생체 내(In vivo) 실험으로 돼지에 기초사료와 함께 프로테아제 및 베타인 염산염을 첨가하여 급이한 경우 분뇨에서 아산화질소 배출량이 감소됨으로써 온실가스 배출이 저감되는 효과가 있음을 확인함으로써 본 발명을 완성하였다.In detail, an in vitro gastrointestinal/intestinal digestion model for pigs was designed and amino acid digestibility was measured in vitro using this model. As a result, the treatment group in which protease and betaine hydrochloride were added as feed additives had a significantly higher (P < 0.05) It was confirmed that protein and amino acid digestibility was shown. In addition, in vivo experiments confirmed that when protease and betaine hydrochloride were added to pigs along with basic feed, the amount of nitrous oxide in the manure was reduced, thereby reducing greenhouse gas emissions. The invention was completed.
따라서 본 발명의 목적은 단위동물에 급이하는 경우 단백질 이용률을 효과적으로 증진시키고 및 온실가스 배출을 저감시킬 수 있는 사료첨가제 조성물을 제공하는 것이다.Therefore, the purpose of the present invention is to provide a feed additive composition that can effectively increase protein utilization and reduce greenhouse gas emissions when fed to monogastric animals.
본 발명의 다른 목적은 단위동물에 급이하는 경우 단백질 이용률을 효과적으로 증진시키고 및 온실가스 배출을 저감시킬 수 있는 배합사료 조성물을 제공하는 것이다.Another object of the present invention is to provide a mixed feed composition that can effectively increase protein utilization and reduce greenhouse gas emissions when fed to monogastric animals.
본 발명의 또 다른 목적은 상기 배합사료 조성물을 이용하여 분뇨로부터 발생하는 아산화질소 배출을 효과적으로 저감시키는 방법을 제공하는 것이다.Another object of the present invention is to provide a method of effectively reducing nitrous oxide emissions from manure using the mixed feed composition.
상기와 같은 본 발명의 목적을 달성하기 위해서,In order to achieve the purpose of the present invention as described above,
본 발명은 프로테아제 및 베타인 염산염을 유효성분으로 포함하는, 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감을 위한 사료첨가제 조성물을 제공한다.The present invention provides a feed additive composition for improving protein utilization and reducing greenhouse gas emissions in monogastric animals, comprising protease and betaine hydrochloride as active ingredients.
본 발명의 일실시예에 있어서, 상기 프로테아제 및 베타인 염산염은 사료첨가제 조성물 내 1:4 내지 1:20의 중량비로 포함될 수 있다.In one embodiment of the present invention, the protease and betaine hydrochloride may be included in a weight ratio of 1:4 to 1:20 in the feed additive composition.
본 발명의 일실시예에 있어서, 상기 단위동물은 돼지일 수 있다.of the present invention In one embodiment, the unitary animal may be a pig.
본 발명의 일실시예에 있어서, 상기 사료첨가제 조성물은 급이한 가축에서 질소 축적율을 증가시키고 아산화질소 배출량을 감소시킴으로써 온실가스 배출 저감 효과를 가질 수 있다.In one embodiment of the present invention, The feed additive composition can have the effect of reducing greenhouse gas emissions by increasing the nitrogen accumulation rate and reducing nitrous oxide emissions in fed livestock.
또한, 본 발명은 기초사료, 프로테아제 및 베타인 염산염을 유효성분으로 포함하는, 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감을 위한 배합사료 조성물을 제공한다.In addition, the present invention provides a compound feed composition for improving protein utilization and reducing greenhouse gas emissions in monogastric animals, comprising basic feed, protease, and betaine hydrochloride as active ingredients.
본 발명의 일실시예에 있어서, 상기 기초사료는 조단백질 함량이 15중량% 이하인 저단백질 기초사료일 수 있다.In one embodiment of the present invention, the basic feed may be a low-protein basic feed with a crude protein content of 15% by weight or less.
본 발명의 일실시예에 있어서, 상기 프로테아제는 배합사료 조성물 총 100중량%를 기준으로 0.01 내지 0.05중량%로 포함될 수 있다.In one embodiment of the present invention, the protease may be included in an amount of 0.01 to 0.05% by weight based on a total of 100% by weight of the compound feed composition.
본 발명의 일실시예에 있어서, 상기 베타인 염산염은 배합사료 조성물 총 100중량%를 기준으로 0.1 내지 0.3중량%로 포함될 수 있다.In one embodiment of the present invention, the betaine hydrochloride may be included in an amount of 0.1 to 0.3% by weight based on a total of 100% by weight of the mixed feed composition.
본 발명의 일실시예에 있어서, 상기 단위동물은 돼지일 수 있다.In one embodiment of the present invention, the unitary animal may be a pig.
또한, 본 발명은 단위동물에 상기 배합사료 조성물을 급이하는 단계를 포함하는, 분뇨로부터 발생하는 아산화질소 배출을 저감시키는 방법을 제공한다.In addition, the present invention provides a method for reducing nitrous oxide emissions from excrement, comprising feeding the mixed feed composition to monogastric animals.
본 발명에 따른 사료첨가제 조성물은 단위동물(monogastric animal)에 적용하는 경우 단위동물의 단백질과 아미노산 이용률을 효과적으로 증진시키고, 단위동물의 분뇨로부터 발생하는 아산화질소 배출을 저감시키는 효과가 우수한바, 단백질 이용률 증진 및 온실가스 배출 저감을 위한 사료첨가제 조성물로서 유용하게 사용될 수 있다.When applied to monogastric animals, the feed additive composition according to the present invention effectively improves the protein and amino acid utilization of monogastric animals and is excellent in reducing nitrous oxide emissions from monogastric animals' excrement, thereby increasing protein utilization. It can be usefully used as a feed additive composition for improving and reducing greenhouse gas emissions.
도 1은 돼지 체외 위·장관 소화모델 모식도이다.Figure 1 is a schematic diagram of an in vitro gastrointestinal and intestinal digestion model in pigs.
본 발명은 프로테아제 및 베타인 염산염을 유효성분으로 포함하는, 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감을 위한 사료첨가제 조성물을 제공한다.The present invention provides a feed additive composition for improving protein utilization and reducing greenhouse gas emissions in monogastric animals, comprising protease and betaine hydrochloride as active ingredients.
본 발명에서 상기 ‘프로테아제(protease)’는 단백질 분해 효소로서 단백질을 구성하고 있는 아미노산 간의 펩타이드 결합을 가수분해하는 효소로, 옥수수-대두박 사료와 같은 식물성 사료 원료 내 단백질 항영양인자를 분해하여 단백질의 활용을 높이는 역할을 할 수 있다.In the present invention, the 'protease' is a proteolytic enzyme that hydrolyzes peptide bonds between amino acids constituting proteins, and decomposes protein anti-nutritional factors in plant feed raw materials such as corn-soybean meal to utilize the protein. It can play a role in increasing .
본 발명에서 상기 ‘베타인 염산염(Betaine hydrochloride)’은 곡물 및 기타 음식에서 발견되는 비타민과 유사한 물질로 베타인의 산성 형태이다. 베타인은 메틸기(CH3-)가 결합되어 있는 트리메틸글리이신의 형태로 가축 사료 내 메티오닌이나 콜린 등의 요구량을 줄이며 체내에서 아미노산, 지질대사 및 항체형성 등에 중요한 역할로 작용한다.In the present invention, ‘Betaine hydrochloride’ is a substance similar to vitamins found in grains and other foods and is an acidic form of betaine. Betaine is a form of trimethylglycine with a methyl group (CH3-) attached to it. It reduces the requirement for methionine and choline in livestock feed and plays an important role in amino acid and lipid metabolism and antibody formation in the body.
본 발명에서 상기 ‘단위동물(monogastric animal)’은 위(胃)가 하나 뿐인 동물로서 개, 닭, 돼지 등이 이에 속한다. 단위동물은 소화 기관에서 분비되는 효소의 작용에 전적으로 의지하여 영양소 소화 및 흡수 작용이 이루어지며, 일반적으로 반추위 미생물을 이용하여 소화 작용이 이루어지는 반추 동물과는 반대되는 개념으로 사용된다. 단위동물의 소화기관은 입, 식도, 위, 소장, 대장, 항문의 순서로 되어 있으며, 반추(反芻)동물과는 달리 맹장의 발달을 볼 수 없다.In the present invention, the ‘monogastric animal’ refers to an animal with only one stomach and includes dogs, chickens, pigs, etc. Monogastric animals rely entirely on the action of enzymes secreted from the digestive system to digest and absorb nutrients, and are generally used as the opposite of ruminants, whose digestion is accomplished using rumen microorganisms. The digestive system of monogastric animals consists of the mouth, esophagus, stomach, small intestine, large intestine, and anus in that order, and unlike ruminants, the development of the cecum is not visible.
본 발명의 일실시예에 있어서, 상기 단위동물은 돼지일 수 있다.In one embodiment of the present invention, the unitary animal may be a pig.
본 발명에서 상기 프로테아제 및 베타인 염산염은 사료첨가제 조성물 내 1:4 내지 1:20의 중량비로 포함될 수 있다.In the present invention, the protease and betaine hydrochloride may be included in a weight ratio of 1:4 to 1:20 in the feed additive composition.
본 발명자는 단위동물에서 단백질과 아미노산의 이용률을 증진시키고 온실가스 배출을 저감시킬 수 있는 사료첨가제를 발명하고자 노력하였으며. 그 결과 사료첨가제로 프로테아제 및 베타인 염산염을 급이한 경우 가축에서 질소 축적율을 증가시키고 아산화질소 배출량을 감소시킴으로써 온실가스 배출 저감 효과를 갖는 것을 확인하였다.The present inventor sought to invent a feed additive that could improve the utilization of proteins and amino acids in monogastric animals and reduce greenhouse gas emissions. As a result, it was confirmed that feeding protease and betaine hydrochloride as feed additives had the effect of reducing greenhouse gas emissions by increasing the nitrogen accumulation rate and reducing nitrous oxide emissions in livestock.
자세하게는, 본 발명자는 돼지 체외 위·장관 소화모델을 설계하고 이를 이용한 실험관 내(In vitro) 아미노산 소화율 측정하였으며, 그 결과 사료첨가제로 프로테아제 및 베타인 염산염을 첨가한 처리구에서 다른 처리구 대비 유의적으로 높은 (P < 0.05) 단백질 및 아미노산 소화율을 보여주는 것을 확인하였다. 뿐만 아니라, 생체 내(In vivo) 실험으로 돼지에 기초사료와 함께 프로테아제 및 베타인 염산염을 첨가하여 급이한 경우 분뇨에서 아산화질소 배출량이 감소됨으로써 온실가스 배출이 저감되는 효과가 있음을 확인하였다. In detail, the present inventor designed an in vitro gastrointestinal/intestinal digestion model in pigs and measured in vitro amino acid digestibility using this model. As a result, the treatment group with protease and betaine hydrochloride added as a feed additive significantly improved compared to other treatments. It was confirmed that high (P < 0.05) protein and amino acid digestibility was observed. In addition, in vivo experiments confirmed that when protease and betaine hydrochloride were added to pigs along with basic feed, nitrous oxide emissions from manure were reduced, thereby reducing greenhouse gas emissions.
따라서 본 발명의 사료첨가제 조성물은 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감 용도를 가지는 사료첨가제 조성물으로, 프로테아제 및 베타인 염산염을 유효성분으로 포함하는 것을 그 특징으로 한다.Therefore, the feed additive composition of the present invention is a feed additive composition for the purpose of improving protein utilization and reducing greenhouse gas emissions in monogastric animals, and is characterized by containing protease and betaine hydrochloride as active ingredients.
또한, 본 발명은 기초사료, 프로테아제 및 베타인 염산염을 유효성분으로 포함하는, 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감을 위한 배합사료 조성물을 제공한다.In addition, the present invention provides a compound feed composition for improving protein utilization and reducing greenhouse gas emissions in monogastric animals, comprising basic feed, protease, and betaine hydrochloride as active ingredients.
본 발명에서 상기 ‘기초사료’란 가축의 종류에 따라 급여되는 일반적인 사료조성물로 정의할 수 있다. 이러한 기초사료는 가축의 종류에 따라서 그 필요한 성분 및 조성 그리고 적합한 조성 비율이 모두 당업계에 공지되어 있으며, 또한 시판되고 있기 때문에 당업자라면 누구나 매우 용이하게 이를 제조 또는 구입하여 사용할 수 있을 것이다.In the present invention, the ‘basic feed’ can be defined as a general feed composition fed according to the type of livestock. The necessary ingredients, composition, and appropriate composition ratio of this basic feed are all known in the art depending on the type of livestock, and since it is commercially available, anyone skilled in the art will be able to manufacture or purchase and use it very easily.
구체적으로 기초사료는 그 구성성분으로서 가축의 종류에 따라 적합한 전분함유 물질, 단백질 함유 물질, 지방 함유 물질, 비타민 함유 물질, 무기질 함유 물질, 산화 방지 물질, 항생제 등의 일부 또는 전부를 포함하여 구성될 수 있다.Specifically, the basic feed may be composed of some or all of starch-containing substances, protein-containing substances, fat-containing substances, vitamin-containing substances, mineral-containing substances, antioxidant substances, antibiotics, etc. suitable for the type of livestock. You can.
상기 전분 함유 물질은 그것이 사육동물의 성장을 유지할 수 있는 한 모두 사육동물의 일반 사료에 포함될 수 있는데, 이러한 전분 함유 물질은 일반적으로 옥수수, 콩, 밀, 수수, 보리, 귀리 등으로부터 얻어진다. 적합한 전분 함유 물질로서는 옥수수 분쇄물이나 분말, 귀리 분쇄물이나 분말, 콩 분쇄물이나 분말, 밀 분쇄물이나 분 말 등을 들 수 있다. 바람직한 적합한 전분 함유 물질은 귀리 분말, 옥수수 분쇄물, 밀 분쇄물, 콩 분말 등이다. 이러한 전분 함유 물질은 사육동물의 성장을 효과적으로 유지할 수 있다면, 가축의 기초사료에 포함되는 그것의 농도는 특정 범위로 제한되지 않는다. 일반적으로 전분 함유 물질은 약 30 내지 약 80 중량 %, 바람직하게는 약 40 내지 약 70 중량 %, 가장 바람직하게는 50 내지 70 중량 %의 범위로 가축의 기초사료에 포함될 수 있다.The above starch-containing substances can all be included in the general feed of farmed animals as long as they can maintain the growth of farmed animals. These starch-containing substances are generally obtained from corn, soybeans, wheat, sorghum, barley, oats, etc. Suitable starch-containing materials include corn meal or meal, oat meal or meal, soybean meal or meal, and wheat meal or meal. Preferred suitable starch-containing materials are oat meal, corn meal, wheat meal, soybean meal, etc. If these starch-containing substances can effectively maintain the growth of farmed animals, their concentration included in basic feed for livestock is not limited to a specific range. In general, the starch-containing material may be included in the basic feed for livestock in the range of about 30 to about 80% by weight, preferably about 40 to about 70% by weight, and most preferably 50 to 70% by weight.
단백질 함유 물질도 사육동물의 성장을 유지시킬 수 있는 한, 가축의 기초사료에 포함될 수 있다. 경제적인 이유로 어분, 콩 분말 등과 같은 단백질 함유 물질이 사용되고 있는데, 다른 것들로서는 콩 단백질 농축물, 혈분, 혈장 단백질, 탈지유 건체물, 유(乳) 단백질 농축물, 옥수수 글루텐(Gluten) 분말, 밀 글루텐 분말, 이스트, 해바라기씨 분말 등을 들 수 있다. 바람직한 단백질 함유 물질은 어분, 혈분, 혈장 단백질, 콩 분말 등이다. 단백질 함유 물질도 사육동물의 성장을 효과적으로 유지할 수 있는 한, 사육동물의 일반 사료에 포함되는 그것의 농도는 중요하지 않다. 일반적으로, 단백질 함유 물질은 약 10 내지 약 50 중량 %, 바람직하게는 약 15 내지 약 40 중량 %, 가장 바람직하게는 18 내지 30 중량 %의 범위로 본 발명의 사료 조성물에 포함될 수 있다.Protein-containing substances can also be included in basic feed for livestock as long as they can maintain the growth of farmed animals. For economic reasons, protein-containing materials such as fishmeal, soybean meal, etc. are used; others include soy protein concentrate, blood meal, plasma protein, skim milk dry matter, milk protein concentrate, corn gluten powder, and wheat gluten. Examples include powder, yeast, and sunflower seed powder. Preferred protein-containing materials include fish meal, blood meal, plasma protein, and soybean meal. As long as protein-containing substances can effectively maintain the growth of farmed animals, their concentration in the regular feed of farmed animals is not critical. Generally, the protein-containing material may be included in the feed composition of the present invention in the range of about 10 to about 50% by weight, preferably about 15 to about 40% by weight, and most preferably 18 to 30% by weight.
가축의 기초사료에는 지방 함유 물질도 포함될 수 있다. 적합한 지방 함유 물질로서는 라드(Lard), 우지, 콩기름, 레시틴, 코코넛유 등을 포함하나 이에 한정되지는 않는다. 바람직한 지방 함유 물질은 콩기름, 코코넛유 및 라드 등이다. 지방 함유 물질도 사육동물의 성장을 유지할 수 있는 한 사육동물의 일반 사료에 포함되는 그것의 농도는 중요하지 않다. 지방 함유 물질은 일반적으로 약 2 내지 약 20 중량 %, 바람직하게는 약 4 내지 약 15 중량 %, 가장 바람직하게는 6 내지 12 중량 %의 범위로 본 발명의 사료 조성물에 존재할 수 있다.Base feed for livestock may also contain fat-containing substances. Suitable fat-containing materials include, but are not limited to, lard, beef tallow, soybean oil, lecithin, coconut oil, etc. Preferred fat-containing substances include soybean oil, coconut oil, and lard. The concentration of fat-containing substances in the regular feed of farmed animals is not important as long as they can maintain the growth of the farmed animals. The fat-containing material may be present in the feed composition of the present invention generally in the range of about 2 to about 20 weight percent, preferably in the range of about 4 to about 15 weight percent, and most preferably in the range of 6 to 12 weight percent.
가축의 기초사료에는 또한 수용성·지용성 비타민과 미량의 무기물이 포함될 수 있다. 적합한 비타민으로서는 비타민 A, 비타민 D, 비타민 E, 비타민 K, 리보플라빈, 판토텐산(Pantothenic Acid), 나이아신, 비타민 B12, 엽산, 비오틴, 비타민 C 등을 들 수 있다. 적합한 미량의 무기물로서는 구리, 아연, 요오드, 셀렌, 망간, 철, 코발트, 등을 들 수 있다. 여기서 "미량"이 의미하는 바는 가축의 기초사료에 사용된 이들 성분들의 양이 다른 성분들의 양보다 실질적으로 적은 것을 의미한다. 일반적으로, 비타민이나 무기질이 미량으로 가축의 기초사료에 포함될 경우에 그 조성물의 총 중량을 기준으로 하여 약 0.0001 내지 약 5 중량 %로 존재할 수 있다.Base feed for livestock may also contain water-soluble and fat-soluble vitamins and trace minerals. Suitable vitamins include vitamin A, vitamin D, vitamin E, vitamin K, riboflavin, pantothenic acid, niacin, vitamin B12, folic acid, biotin, and vitamin C. Suitable trace minerals include copper, zinc, iodine, selenium, manganese, iron, cobalt, and the like. Here, “trace amounts” means that the amounts of these ingredients used in basic feed for livestock are substantially less than the amounts of other ingredients. Generally, when vitamins or minerals are included in trace amounts in basic feed for livestock, they may be present in an amount of about 0.0001 to about 5% by weight based on the total weight of the composition.
가축의 기초사료에는 또한 BHT(Butylated Hydroxytoluene), BHA(Butylated Hydroxyanisole), 비타민 E, 아스코르브산 등의 산화방지물질이 포함될 수 있으며, 이들 산화방지물질은 극소량, 즉 약 0.0001 내지 약 1 중량 %로 포함되는 것이 바람직하다.Basic feed for livestock may also contain antioxidants such as BHT (Butylated Hydroxytoluene), BHA (Butylated Hydroxyanisole), Vitamin E, and ascorbic acid, and these antioxidants are contained in very small amounts, that is, about 0.0001 to about 1% by weight. It is desirable to be
본 발명의 하기 실시예에서는 양돈용 기초사료를 사용하였다. 조단백질의 함량(16.8%, 14.8%)에 따라 고단백질 또는 저단백질 기초사료를 사용하였다.In the following examples of the present invention, basic feed for pig farming was used. High-protein or low-protein basic feed was used depending on the crude protein content (16.8%, 14.8%).
본 발명의 배합사료 조성물은 기초사료 100중량%에 대하여 프로테아제를 0.01 내지 0.05중량%로 포함할 수 있으며, 베타인 염산염을 0.1 내지 0.3중량%로 포함할 수 있다.The mixed feed composition of the present invention may contain 0.01 to 0.05% by weight of protease and 0.1 to 0.3% by weight of betaine hydrochloride based on 100% by weight of basic feed.
또한, 본 발명은 단위동물에 상기 배합사료 조성물을 급이하는 단계를 포함하는, 분뇨로부터 발생하는 아산화질소 배출을 저감시키는 방법을 제공한다.In addition, the present invention provides a method for reducing nitrous oxide emissions from excrement, comprising feeding the mixed feed composition to monogastric animals.
이하, 실시예를 통하여 본 발명을 보다 상세히 설명하고자 한다. 이들 실시예는 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail through examples. These examples are for illustrating the present invention in more detail, and the scope of the present invention is not limited to these examples.
<실시예 1><Example 1>
아미노산 소화율 증진을 위한 배합사료 설계Design of mixed feed to improve amino acid digestibility
본 발명자는 돼지의 사료 섭취 시 아미노산 소화율을 증진시킬 수 있는 방안을 모색하기 위하여, 기초사료에 프로테아제(PT125, 유진바이오) 및/또는 베타인 염산염(㈜이앤티)을 사료첨가제로 배합하여 실험사료로 준비하였다(표 1 및 2 참조). 기초사료에 배합되는 사료첨가제의 비율은 하기 표 2에서 자세히 나타내었다.In order to find a way to improve amino acid digestibility when consuming feed in pigs, the present inventor mixed protease (PT125, Eugene Bio) and/or betaine hydrochloride (E&T Co., Ltd.) as feed additives in basic feed to produce experimental feed. It was prepared (see Tables 1 and 2). The ratio of feed additives mixed in basic feed is shown in detail in Table 2 below.
(%)Ingredients
(%)
2Provided per kg of complete diet without Zinc: Cu (as CuSO4·5H2O), 12 mg; Mn (as MnO2), 8 mg; I (as KI), 0.28 mg; and Se (as Na2SeO3·5H2O), 0.15 mg. 1 Provided per kg of complete diet: vitamin A, 11,025 IU; vitamin D3, 1103 IU; vitamin E, 44 IU; vitamin K, 4.4 mg; riboflavin, 8.3 mg; niacin, 50 mg; thiamine, 4 mg; d-pantothenic, 29 mg; and vitamin B12, 33 mg.
2 Provided per kg of complete diet without Zinc: Cu (as CuSO 4 ·5H 2 O), 12 mg; Mn (as MnO 2 ), 8 mg; I (as KI), 0.28 mg; and Se (as Na 2 SeO 3 ·5H 2 O), 0.15 mg.
(중량%)Mixed raw materials
(weight%)
100Basic A
100
100Basic B
100
100Basic B
100
100Basic B
100
100Basic B
100
<실시예 2><Example 2>
돼지 체외 위·장관 소화모델 설계 및 이를 이용한 실험관 내(Design of pig in vitro gastrointestinal and intestinal digestion model and in vitro using it ( In vitroIn vitro ) 아미노산 소화율 측정) Amino acid digestibility measurement
<2-1> 돼지 체외 위·장관 소화모델 설계<2-1> Design of pig in vitro gastrointestinal and intestinal digestion model
실험관 내(In vitro) 아미노산 소화율을 측정하기 위한 돼지 체외 위·장관 소화모델은 Bosien and Fernndez(1997)의 방법을 기반으로 설계하였다(도 1 참조).The porcine in vitro gastrointestinal digestion model for measuring in vitro amino acid digestibility was developed by Bosien and Fern. It was designed based on the method of ndez (1997) (see Figure 1).
먼저, 사료 입자는 1mm 보다 작게 설정하였으며, 실험관 내(In vitro) 아미노산 소화율 측정을 위해 위-소장-대장 모델 순으로 39℃에서 각각 2시간, 4시간, 18시간 배양하였다.First, the feed particles were set to be smaller than 1 mm, and in order to measure amino acid digestibility in vitro , the stomach-small intestine-large intestine model was cultured at 39°C for 2 hours, 4 hours, and 18 hours, respectively.
1 단계로 위의 소화에 사용된 배양액은 25ml 완충용액 (0.1M, pH=6.0)과 10ml 염산 (0.2M)을 첨가한 뒤 pH=2 적정 후, 1ml 펩신 용액 (250units/mg, P7000, Sigma Aldrich, USA)과 0.5ml 클로람페니콜 (HPLC grade, C0378, Sigma Aldrich, USA)을 첨가하였다.In step 1, the culture medium used for the above digestion was added with 25ml buffer solution (0.1M, pH=6.0) and 10ml hydrochloric acid (0.2M), titrated to pH=2, and then added with 1ml pepsin solution (250units/mg, P7000, Sigma). Aldrich, USA) and 0.5ml chloramphenicol (HPLC grade, C0378, Sigma Aldrich, USA) were added.
2 단계로 소장의 소화에 사용된 배양액은 10ml 완충용액 (0.2M, pH=6.8)에 5ml 수산화나트륨 (0.6M)으로 pH=6.8 적정 후, 1ml 판크레아틴 용액 (porcine grade Ⅳ, Sigma Aldrich, USA)을 첨가하였다.The culture medium used for digestion of the small intestine in step 2 was titrated to pH=6.8 with 5ml sodium hydroxide (0.6M) in 10ml buffer solution (0.2M, pH=6.8), and then added to 1ml pancreatin solution (porcine grade Ⅳ, Sigma Aldrich, USA). ) was added.
3 단계로 대장의 소화에 사용된 배양액은 10ml EDTA 용액 (0.2M)에 30% 아세트산 용액으로 pH=4.8로 적정 후, 0.5ml 비스코자임 (Viscozyme L, V2010, sigma Aldrich, USA)을 첨가하였다.The culture medium used for digestion of the large intestine in step 3 was titrated to pH=4.8 with 30% acetic acid solution in 10ml EDTA solution (0.2M), and then 0.5ml Viscozyme (Viscozyme L, V2010, sigma Aldrich, USA) was added.
<2-2> 아미노산 소화율 측정<2-2> Measurement of amino acid digestibility
실험사료와 실험관 내(In vitro) 아미노산 소화물은 동결건조 후 0.1% 페놀이 함유된 6N 염산(HCL)으로 가수분해한 후 고성능-액체크로마토그래피(HPLC)를 이용하여 아미노산 함량을 분석하였다.The experimental feed and in vitro amino acid digests were freeze-dried, hydrolyzed with 6N hydrochloric acid (HCL) containing 0.1% phenol, and then analyzed for amino acid content using high-performance liquid chromatography (HPLC).
단백질 함량은 AOAC (AOAC official method 968.06)에 등재되어 있는 듀마스법 (Combustion method)을 이용하여 질소함량을 구한 후 질소계수(6.25)를 이용해 분석하였다.Protein content was analyzed using the nitrogen coefficient (6.25) after calculating the nitrogen content using the Dumas method (Combustion method) listed in AOAC (AOAC official method 968.06).
본 실험은 5개의 처리구를 각각 5반복으로 수행하였으며, 실험관 내(In vitro) 소화율 결과 값은 아래의 식을 이용하여 계산하였다.This experiment was performed with 5 repetitions for each of the 5 treatments, and the in vitro digestibility results were calculated using the formula below.
그 결과 하기 표 3에서 나타낸 바와 같이, T3 처리구는 PC, NC, 및 T2 처리구 대비 유의적으로 높은 (P < 0.05) 조단백질 소화율을 보이는 것을 나타났다.As a result, as shown in Table 3 below, the T3 treatment group showed significantly higher (P < 0.05) crude protein digestibility than the PC, NC, and T2 treatments.
한편, 아미노산별 소화율을 확인한 결과 라이신, 아르기닌 및 메티오닌+시스테인의 경우 T1 및 T3 처리구에서 다른 처리구 대비 유의적으로 높은 (P < 0.05) 소화율을 나타냈으며; 트레오닌 및 이소류신의 경우 T1 및 T3 처리구에서 PC 처리구 대비 유의적으로 높은 (P < 0.05) 소화율을 나타냈고; 발린의 경우 T3 처리구에서는 다른 처리구 대비 유의적으로 높은 (P < 0.05) 소화율을 나타냈으며; 류신의 경우 PC 처리구에서 다른 처리구 대비 유의적으로 낮은 (P < 0.05) 소화율을 보인 반면, T1 및 T3 처리구에서 다른 처리구 대비 유의적으로 높은 (P < 0.05) 소화율을 나타내었다.Meanwhile, as a result of checking the digestibility of each amino acid, the digestibility of lysine, arginine, and methionine + cysteine was significantly higher (P < 0.05) in the T1 and T3 treatments compared to the other treatments; In the case of threonine and isoleucine, digestibility was significantly higher (P < 0.05) in the T1 and T3 treatments compared to the PC treatment; In the case of valine, the T3 treatment showed a significantly higher (P < 0.05) digestibility than the other treatments; In the case of leucine, the PC treatment showed a significantly lower (P < 0.05) digestibility than the other treatments, while the T1 and T3 treatments showed a significantly higher (P < 0.05) digestibility than the other treatments.
상기와 같은 결과를 통해, 돼지 육성돈 사료 (조단백질 함량 14.8%) 내 프로테아제 0.05중량% 및 베타인 염산염 0.2중량% 혼합 첨가는 고단백질 (조단백질 16.8%) 및 저단백질 (14.8%) 사료와 비교하여 단백질 및 아미노산 소화율 향상 효과를 보이는 것을 알 수 있었다.From the above results, the addition of 0.05% by weight of protease and 0.2% by weight of betaine hydrochloride in pig feed (crude protein content 14.8%) increased protein compared to high protein (crude protein 16.8%) and low protein (14.8%) feed. It was found that it showed an effect of improving amino acid digestibility.
참고로, 하기 표 3에서 ‘메티오닌+시스테인’은 황함유 아미노산을 표기한 것이다.For reference, in Table 3 below, ‘methionine + cysteine’ indicates a sulfur-containing amino acid.
+시스테인methionine
+Cysteine
NC : 조단백질 14.8%의 기초사료 (기초 B)
T1 : NC + 0.05% 프로테아제
T2 : NC + 0.2% 베타인 염산염
T3 : NC + 0.05% 프로테아제 + 0.2% 베타인 염산염
a-c 각 처리구 내 서로 다른 머릿글자는 유의적인 차이가 있음 (P<0.05).PC: Basic feed with 16.8% crude protein (Basic A)
NC: Basic feed with 14.8% crude protein (Basic B)
T1: NC + 0.05% protease
T2: NC + 0.2% betaine hydrochloride
T3: NC + 0.05% protease + 0.2% betaine hydrochloride
ac Different initials within each treatment group have significant differences (P<0.05).
<실시예 3><Example 3>
질소 축적량 및 아산화질소 배출량 측정을 위한 배합사료 설계Formulated feed design for measuring nitrogen accumulation and nitrous oxide emissions
생체 내(In vivo) 질소 축적량 및 아산화질소 배출량 측정을 위하여 조단백질 함량별 기초사료(기초 A: 조단백질 16.8%, 기초 B: 조단백질 14.8%)에 프로테아제 및/또는 베타인 염산염을 사료첨가제로 배합하여 실험사료로 준비하였다(표 1 및 4 참조). 기초사료에 배합되는 사료첨가제의 비율은 하기 표 4에서 자세히 나타내었다.To measure nitrogen accumulation and nitrous oxide emissions in vivo , experiments were conducted by mixing protease and/or betaine hydrochloride as feed additives in basic feed by crude protein content (basic A: 16.8% crude protein, basal B: 14.8% crude protein). It was prepared as feed (see Tables 1 and 4). The ratio of feed additives mixed in basic feed is shown in detail in Table 4 below.
(중량%)Mixed raw materials
(weight%)
100Basic B
100
100Basic A
100
100Basic B
100
100Basic B
100
100Basic A
100
100Basic A
100
<실시예 4><Example 4>
생체 내(In vivo ( In vivoIn vivo ) 질소 축적량 및 아산화질소 배출량 측정) Measurement of nitrogen accumulation and nitrous oxide emissions
생체 내(In vivo) 질소 축적량 및 아산화질소 배출량 측정을 위하여 개시 체중이 18.74 ± 0.39 kg인 3원 교잡종 [(Landrace × Yorkshire) × Duroc] 육성돈 60두를 공시하여 13주간 사양시험을 진행하였으며, 모든 돼지는 충북대학교의 동물실험관리위원회의 가이드라인에 따라 관리하였다. 실험에서 사용한 육성돈 60두는 ㈜세이리팜으로부터 제공받았다.To measure nitrogen accumulation and nitrous oxide emissions in vivo , a 13-week feeding test was conducted on 60 three-way crossbreed [(Landrace × Yorkshire) × Duroc] pigs with a starting weight of 18.74 ± 0.39 kg. Pigs were managed according to the guidelines of the Animal Experiment Management Committee of Chungbuk National University. The 60 raised pigs used in the experiment were provided by Seiri Farm Co., Ltd.
본 실험은 <실시예 3>에서 나타낸 배합사료의 종류에 따라 6개의 처리구로 나눈 후 처리구당 10두씩 사육하였다. 톱밥 돈사에서 물과 사료를 자유 채식시키며 사양관리를 진행하였으며, 돈분 성상조사를 위해 비육돈 시험종료 전 각 처리구별 6마리에서 분을 채취하여 성상을 조사하였다.This experiment was divided into 6 treatment groups according to the type of mixed feed shown in <Example 3>, and 10 heads were reared per treatment group. Feeding management was carried out in sawdust pig pens with free access to water and feed. To investigate the properties of pig feces, feces were collected from 6 pigs in each treatment group before the end of the finishing pig test to investigate the properties.
질소 축적율 결과값은 아래의 식을 이용하여 계산하였다.The nitrogen accumulation rate result was calculated using the formula below.
질소 축적율 (%)= NI - [(NF + NU)/NI × 100Nitrogen accumulation rate (%) = NI - [(NF + NU)/NI × 100
상기 식에서 NI는 질소섭취량을 의미하며, NF는 분으로 배출된 질소를 의미하고, NU는 뇨로 배출된 질소를 의미한다.In the above formula, NI means nitrogen intake, NF means nitrogen excreted in feces, and NU means nitrogen excreted in urine.
그 결과 하기 표에서 나타낸 바와 같이, 사료 내 프로테아제 및 비테인 염산염 첨가 처리구는 다른 처리구 대비 유의적으로 높은 (P < 0.05) 총 질소 축적율을 보여주었다. 특히, 조단백질 함량이 낮은 사료(기초 B) 내 프로테아제 및 비테인 염산염을 첨가한 처리구(t2)는 다른 처리구 대비 유의적으로 낮은 (P < 0.05) 아산화질소 배출량을 보였다.As a result, as shown in the table below, the treatment group adding protease and betaine hydrochloride in the feed showed a significantly higher (P < 0.05) total nitrogen accumulation rate than the other treatments. In particular, the treatment group (t2) in which protease and betaine hydrochloride were added to feed with low crude protein content (basal B) showed significantly lower (P < 0.05) nitrous oxide emissions compared to other treatments.
상기와 같은 결과를 통해, 조단백질 함량이 낮은 사료(기초 B)를 기초사료로 하여 여기에 사료첨가제로 비테인 염산염 및 프로테아제를 배합하여 사용하는 경우, 돼지 생체 내에 질소 축적율을 증가시키는 효과를 보임으로써 아산화질소 배출이 저감되었고 이로 인해 분뇨에서 발생하는 온실가스인 아산화질소가 저감되는 효과를 나타냈다.Based on the above results, when using feed (Basal B) with low crude protein content as the basic feed and mixing it with betaine hydrochloride and protease as feed additives, the effect of increasing the nitrogen accumulation rate in the pig's body was shown. As a result, nitrous oxide emissions were reduced, which had the effect of reducing nitrous oxide, a greenhouse gas generated from excrement.
PC : 조단백질 16.8%의 기초사료 (기초 A)
t1 : NC + 0.0125% 프로테아제
t2 : NC + 0.0125% 프로테아제 + 0.2% 베타인 염산염
t3 : PC + 0.0125% 프로테아제
t4 : PC + 0.0125% 프로테아제 + 0.2% 베타인 염산염
a-c 각 처리구 내 서로 다른 머릿글자는 유의적인 차이가 있음 (P<0.05).NC: Basic feed with 14.8% crude protein (Basic B)
PC: Basic feed with 16.8% crude protein (Basic A)
t1: NC + 0.0125% protease
t2: NC + 0.0125% protease + 0.2% betaine hydrochloride
t3: PC + 0.0125% protease
t4: PC + 0.0125% protease + 0.2% betaine hydrochloride
ac Different initials within each treatment group have significant differences (P<0.05).
결론적으로, 돼지 육성돈 사료(조단백질 함량 14.8%) 내 프로테아제 및 비테인 염산염의 혼합 첨가는 고단백질 (조단백질 16.8%) 및 저단백질 (14.8%) 사료와 비교하여 실험관 내(In vitro) 및 생체 내(In vivo) 실험에서 단백질 및 아미노산 소화율 향상 효과를 보이며, 총 질소 축적율 증가 및 아산화질소 배출량 감소 효과를 통해 온실가스 배출 저감에 긍정적인 영향을 미치는 것으로 판다되었다.In conclusion, the mixed addition of protease and betaine hydrochloride in swine feed (crude protein content 14.8%) was effective in vitro and in vivo compared to high-protein (crude protein 16.8%) and low-protein (14.8%) feeds. In vivo ) experiments show that it improves protein and amino acid digestibility, and is believed to have a positive effect on reducing greenhouse gas emissions by increasing the total nitrogen accumulation rate and reducing nitrous oxide emissions.
이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.So far, the present invention has been examined focusing on its preferred embodiments. A person skilled in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a restrictive perspective. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the equivalent scope should be construed as being included in the present invention.
Claims (10)
상기 프로테아제 및 베타인 염산염은 사료첨가제 조성물 내 1:4 내지 1:20의 중량비로 포함되는 것을 특징으로 하는, 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감을 위한 사료첨가제 조성물.According to paragraph 1,
A feed additive composition for improving protein utilization and reducing greenhouse gas emissions in monogastric animals, characterized in that the protease and betaine hydrochloride are contained in a weight ratio of 1:4 to 1:20 in the feed additive composition.
상기 단위동물은 돼지인 것을 특징으로 하는, 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감을 위한 사료첨가제 조성물.According to paragraph 1,
A feed additive composition for improving protein utilization and reducing greenhouse gas emissions in monolithic animals, wherein the monolithic animal is a pig.
상기 사료첨가제 조성물은 급이한 가축에서 질소 축적율을 증가시키고 아산화질소 배출량을 감소시킴으로써 온실가스 배출 저감 효과를 갖는 것을 특징으로 하는, 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감을 위한 사료첨가제 조성물.According to paragraph 1,
The feed additive composition is a feed additive composition for improving protein utilization and reducing greenhouse gas emissions in monogastric animals, characterized in that it has the effect of reducing greenhouse gas emissions by increasing nitrogen accumulation rate and reducing nitrous oxide emissions in fed livestock. .
상기 기초사료는 조단백질 함량이 15중량% 이하인 저단백질 기초사료인 것을 특징으로 하는, 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감을 위한 배합사료 조성물.According to clause 5,
The basic feed is a low-protein basic feed with a crude protein content of 15% by weight or less. A compound feed composition for improving protein utilization and reducing greenhouse gas emissions in unitary animals.
상기 프로테아제는 배합사료 조성물 총 100중량%를 기준으로 0.01 내지 0.05중량%로 포함되는 것을 특징으로 하는, 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감을 위한 배합사료 조성물.According to clause 5,
A compound feed composition for improving protein utilization and reducing greenhouse gas emissions in monogastric animals, characterized in that the protease is contained in an amount of 0.01 to 0.05% by weight based on a total of 100% by weight of the compound feed composition.
상기 베타인 염산염은 배합사료 조성물 총 100중량%를 기준으로 0.1 내지 0.3중량%로 포함되는 것을 특징으로 하는, 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감을 위한 배합사료 조성물.According to clause 5,
A compound feed composition for improving protein utilization and reducing greenhouse gas emissions in monogastric animals, characterized in that the betaine hydrochloride is contained in an amount of 0.1 to 0.3% by weight based on a total of 100% by weight of the compound feed composition.
상기 단위동물은 돼지인 것을 특징으로 하는, 단위동물에서 단백질 이용률 증진 및 온실가스 배출 저감을 위한 배합사료 조성물.According to clause 5,
A compound feed composition for improving protein utilization and reducing greenhouse gas emissions in monolithic animals, wherein the monolithic animal is a pig.
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