KR20240063263A - Multi-Purpose Powder Containing Unripe Bokbunja Extract - Google Patents

Multi-Purpose Powder Containing Unripe Bokbunja Extract Download PDF

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KR20240063263A
KR20240063263A KR1020220142276A KR20220142276A KR20240063263A KR 20240063263 A KR20240063263 A KR 20240063263A KR 1020220142276 A KR1020220142276 A KR 1020220142276A KR 20220142276 A KR20220142276 A KR 20220142276A KR 20240063263 A KR20240063263 A KR 20240063263A
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composition
extract
prepared
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powder
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이수정
최혜란
권지웅
류태호
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재단법인 고창식품산업연구원
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    • A—HUMAN NECESSITIES
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    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
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    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
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    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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    • A23V2200/00—Function of food ingredients
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/324—Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23V2250/00—Food ingredients
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    • A23V2300/10—Drying, dehydrating
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying

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Abstract

본 발명은 건조된 복분자 미숙과를 활용하여 다용도 제1파우더를 제조하는 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 다용도 파우더에 관한 것이다.
또한, 상기 복분자 미숙과를 베이스로 하여 식품첨가제, 영양크림등 다양하게 활용될 수 있는 제2파우더를 제조하는 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 다용도 파우더에 관한 것이다.The present invention relates to a multi-purpose powder containing unripe Bokbunja fruit extract, characterized in that the first multi-purpose powder is manufactured using dried unripe Bokbunja fruit.
In addition, it relates to a multi-purpose powder containing unripe Bokbunja fruit extract, characterized in that a second powder that can be used in various ways, such as food additives and nutritional creams, is manufactured based on the unripe Bokbunja fruit.

Description

복분자 미숙과 추출물을 포함하는 다용도 파우더{Multi-Purpose Powder Containing Unripe Bokbunja Extract}{Multi-Purpose Powder Containing Unripe Bokbunja Extract}

본 발명은 건조된 복분자 미숙과를 단독으로 활용하여 다용도 제1파우더를 제조하는 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 다용도 파우더에 관한 것이다. The present invention relates to a multi-purpose powder containing unripe Bokbunja fruit extract, characterized in that the first multi-purpose powder is manufactured by using dried unripe Bokbunja fruit alone.

또한, 상기 복분자 미숙과를 베이스로 하여 식품첨가제, 영양크림등 활용에 있어 더욱 효과가 극대화된 제2파우더를 제조하는 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 다용도 파우더에 관한 것이다.In addition, it relates to a multi-purpose powder containing unripe Bokbunja fruit extract, characterized in that a second powder with greater effectiveness in use as a food additive, nutritional cream, etc. is manufactured using the unripe Bokbunja fruit as a base.

일반적으로 복분자는 고창이 최대 생산지로 총 안토시아닌과 폴리페놀 화합물이 다량 함유되어 있으며 항산화, 혈중지질개선, 전립선비대개 등의 다양한 생리활성에 대한 연구가 다수 보고되어 왔다. Ellagic acid는 다양한 식물에 존재하며 기능성을 갖는 단일성분으로 널리 알려져 있으며 복분자에도 다량으로 함유되어 있다. 따라서 본 발명자는 고창에서 재배되고 있는 R. occidentalis의 미성숙 열매 추출물를 베이스로 하여 다용도로 활용할 수 있는 파우더를 제조하고 자 한다. In general, Gochang is the largest producer of bokbunja, and it contains a large amount of total anthocyanins and polyphenol compounds, and many studies have been reported on various physiological activities such as antioxidant, blood lipid improvement, and prostatic hypertrophy. Ellagic acid exists in various plants and is widely known as a single functional ingredient, and it is also contained in large quantities in bokbunja. Therefore, the present inventor would like to manufacture a powder that can be used for many purposes based on the extract of immature fruit of R. occidentalis cultivated in Gochang.

대한민국 공개특허 10-2015-0116569(공개일자:2015년10월16일)Republic of Korea Open Patent No. 10-2015-0116569 (Publication Date: October 16, 2015) 대한민국 공개특허 10-2013-0104205(공개일자:2013년09월25일)Republic of Korea Public Patent No. 10-2013-0104205 (Publication Date: September 25, 2013)

상기 목적을 달성하기 위해, To achieve the above purpose,

본 발명은 건조된 복분자 미숙과를 활용하여 다용도로 활용될 수 있는 제1파우더를 제조하고,The present invention manufactures a first powder that can be used for many purposes using dried unripe bokbunja fruit,

또한, 상기 복분자 미숙과를 베이스로 하여 식품첨가제, 영양크림등 다양하게 활용될 수 있는 제2파우더를 제조하고, In addition, a second powder that can be used in a variety of ways, such as food additives and nutritional creams, is manufactured based on the unripe bokbunja fruits.

상기 제1파우더 및 제2파우더를 활용하여 식품첨가제 및 영양크림을 제조하는 것을 발명의 목적으로 한다. The purpose of the invention is to manufacture food additives and nutritional creams using the first powder and the second powder.

또한, 본원발명은 다수개의 성분들을 조합하여 식품류, 화장품류 첨가에 있어 그 효과를 극대화하는 것을 발명의 목적으로 한다. In addition, the purpose of the present invention is to maximize the effect of adding multiple ingredients to foods and cosmetics by combining them.

상기 문제를 해결하기 위해, 건조된 복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 또는 물 중 선택되는 어느 1종인 추출용매 70 ~ 99.9 wt%를 혼합하여 제1조성물을 제조하고,In order to solve the above problem, a first composition was prepared by mixing dried unripe bokbunja with 0.01 to 30 wt% and 70 to 99.9 wt% of an extraction solvent selected from 50% ethanol or water,

상기 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제1조성물 추출물을 제조하고, 상기 제1조성물 추출물을 여과하여 여과액을 제조한 후, The first composition was heated and extracted twice for 1 to 5 hours to prepare a first composition extract, and the first composition extract was filtered to prepare a filtrate,

그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제조된 제1파우더인 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 파우더을 제공한다. A powder containing unripe Bokbunja fruit extract is provided, which is the first powder prepared by concentrating the filtrate under reduced pressure, freeze-drying, and pulverizing the filtrate.

또한, 상기 문제를 해결하기 위해, 복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 70 ~ 99.9 wt%를 혼합하여 제조된 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제1조성물 추출물과,In addition, in order to solve the above problem, the first composition prepared by mixing unripe bokbunja with 0.01 to 30 wt% and 70 to 99.9 wt% of 50% ethanol was heated and extracted twice for 1 to 5 hours to prepare the first composition. Composition extract,

용안육 1 ~ 30 wt%, 산두근 1 ~ 30 wt%, 파극천 1 ~ 30 wt%, 파고지 1 ~ 30 wt%, 고량강 1 ~ 30 wt%, 필발 1 ~ 30 wt% 및 석곡 1 ~ 30 wt%을 혼합하여 제조된 제2조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제2조성물 추출물과. Yongan-yuk 1 ~ 30 wt%, Sangeun 1 ~ 30 wt%, Pageukcheon 1 ~ 30 wt%, Pagoji 1 ~ 30 wt%, Goryanggang 1 ~ 30 wt%, Pilbal 1 ~ 30 wt%, and Dendrobium 1 ~ 30 wt%. An extract of the second composition prepared by heating and extracting the second composition prepared by mixing twice for 1 to 5 hours.

매미애플 1 ~ 30 wt%, 플래토니아 1 ~ 30 wt%, 커스터드 사과 1 ~ 30 wt%, 자부티카바 1 ~ 30 wt%, 룰로 1 ~ 30 wt%를 혼합하여 제조된 제3조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제3조성물 추출물과,A third composition prepared by mixing 1 to 30 wt% of cicada apple, 1 to 30 wt% of plateonia, 1 to 30 wt% of custard apple, 1 to 30 wt% of jabuticaba, and 1 to 30 wt% of roulo was mixed in 1 to 3 wt%. An extract of the third composition prepared by heat extraction twice for 5 hours,

상기 제1조성물 추출물과, 제2조성물 추출물과, 제3조성물 추출물을 혼합하여 제조되는 최종추출물;을 여과하여 여과액을 제조한 후, 그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제조된 제2파우더인 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 파우더를 제공한다. The final extract prepared by mixing the extract of the first composition, the extract of the second composition, and the extract of the third composition; was filtered to prepare a filtrate, the filtrate was concentrated under reduced pressure, freeze-dried, and pulverized. Provides a powder containing unripe bokbunja fruit extract, which is characterized as a second powder.

본 발명은 항산화 및 항염증 효과에 뛰어나며, 다양한 형태의 첨가제로 활용될 수 있다. 특히, 화장품 영양 크림, 식품첨가제에 활용될 수 있는데 상기 영양 크림에 활용될 경우 피부의 노화, 주름 예방, 보습에 대하여 큰 효과가 있으며, 상기 식품첨가제의 활용될 경우 활성 산소가 일으키는 손상으로부터 신체가 보호될 수 있도록 하고 많은 질병 예방에 큰 도움을 줄 수 있는 효과를 갖는다. The present invention has excellent antioxidant and anti-inflammatory effects and can be used as various types of additives. In particular, it can be used in cosmetic nutritional creams and food additives. When used in nutritional creams, it has a great effect on skin aging, wrinkle prevention, and moisturizing. When used as food additives, it protects the body from damage caused by active oxygen. It has the effect of providing protection and greatly helping prevent many diseases.

이하, 실시예 A에 따른 구체적인 내용을 살펴보도록 한다. Hereinafter, we will look at specific details according to Example A.

[제1파우더 제조][First powder manufacturing]

건조된 복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 또는 물 중 선택되는 어느 1종인 추출용매 70 ~ 99.9 wt%를 혼합하여 제1조성물을 제조하고,A first composition is prepared by mixing dried unripe bokbunja with 0.01 to 30 wt% and 70 to 99.9 wt% of an extraction solvent selected from 50% ethanol or water,

상기 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제1조성물 추출물을 제조하고, 상기 제1조성물 추출물을 여과하여 여과액을 제조한 후, The first composition was heated and extracted twice for 1 to 5 hours to prepare a first composition extract, and the first composition extract was filtered to prepare a filtrate,

그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제조된 제1파우더인 것을 특징으로 한다. It is characterized as a first powder manufactured by concentrating the filtrate under reduced pressure, freeze-drying, and pulverizing the filtrate.

더욱 구체적으로는, 제1파우더는 건조된 복분자 미숙과 0.01 ~ 30 wt% 및 물 70 ~ 99.9 wt%를 혼합하여 제1조성물을 제조하고, 상기 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제1조성물 추출물을 제조하고, 상기 제1조성물 추출물을 여과하여 여과액을 제조한 후, 그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제조하거나, More specifically, the first powder is prepared by mixing dried unripe bokbunja with 0.01 to 30 wt% and 70 to 99.9 wt% of water to prepare the first composition, and the first composition is heated and extracted twice for 1 to 5 hours. An extract of the first composition is prepared, the extract of the first composition is filtered to prepare a filtrate, the filtrate is concentrated under reduced pressure, freeze-dried, and then pulverized.

또는, 제1파우더는 건조된 복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 70 ~ 99.9 wt%를 혼합하여 제1조성물을 제조하고, 상기 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제1조성물 추출물을 제조하고, 상기 제1조성물 추출물을 여과하여 여과액을 제조한 후, 그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제조된다. Alternatively, the first powder is prepared by mixing dried unripe bokbunja with 0.01 to 30 wt% and 70 to 99.9 wt% of 50% ethanol to prepare a first composition, and heating and extracting the first composition twice for 1 to 5 hours. An extract of the first composition is prepared, the extract of the first composition is filtered to prepare a filtrate, the filtrate is concentrated under reduced pressure, freeze-dried, and pulverized.

더욱 더 구체적으로는, 제1파우더인는 건조된 복분자 미숙과 9 wt%에 추출 용매로 사용된 물 91 wt%을 혼합하여 제1조성물을 제조하고, 상기 제1조성물을 환류냉각장치를 부착한 히팅 맨틀을 이용하여 2시간 동안 2회 가열 추출하여 제1조성물 추출물을 제조하고, 상기 제1조성물 추출물을 여과지(ADVANTEC No. 2, Adventec Toyo Kaisha, Tokyo, Japan)를 이용하여 여과한 뒤 감압농축 후 동결건조하고 분쇄하여 제조되거나,More specifically, the first powder, the first composition, is prepared by mixing dried unripe bokbunja and 9 wt% with 91 wt% of water used as an extraction solvent, and the first composition is heated with a reflux cooling device. An extract of the first composition was prepared by heating extraction twice for 2 hours using a mantle, and the extract of the first composition was filtered using filter paper (ADVANTEC No. 2, Adventec Toyo Kaisha, Tokyo, Japan) and then concentrated under reduced pressure. Manufactured by freeze-drying and grinding,

또는, 제1파우더는 건조된 복분자 미숙과 10 wt%에 추출 용매로 사용된 50%의 에탄올 90 wt%을 혼합하여 제1조성물을 제조하고, 상기 제1조성물을 환류냉각장치를 부착한 히팅 맨틀을 이용하여 2시간 동안 2회 가열 추출하여 제1조성물 추출물을 제조하고, 상기 제1조성물 추출물을 여과지(ADVANTEC No. 2, Adventec Toyo Kaisha, Tokyo, Japan)를 이용하여 여과한 뒤 감압농축 후 동결건조하고 분쇄하여 제조된다. Alternatively, the first powder is prepared by mixing dried unripe bokbunja and 10 wt% with 90 wt% of 50% ethanol used as an extraction solvent to prepare the first composition, and the first composition is placed in a heating mantle equipped with a reflux cooling device. An extract of the first composition was prepared by heating and extracting twice for 2 hours, and the extract of the first composition was filtered using filter paper (ADVANTEC No. 2, Adventec Toyo Kaisha, Tokyo, Japan), concentrated under reduced pressure, and then frozen. It is manufactured by drying and grinding.

이렇게 제조된 제1파우더는 항산화 및 항염증 효과에 뛰어나며,다양한 형태의 첨가제로 활용될 수 있다. 특히, 화장품 영양 크림, 식품첨가제에 활용될 수 있는데 상기 영양 크림에 활용될 경우 피부의 노화, 주름예방에 대하여 큰 효과가 있으며, 상기 식품첨가제의 활용될 경우 활성산소가 일으키는 손상으로부터 신체가 보호될 수 있도록 하고 많은 질병 예방에 큰 도움을 줄 수 있다. The first powder prepared in this way has excellent antioxidant and anti-inflammatory effects and can be used as various types of additives. In particular, it can be used in cosmetic nutritional creams and food additives. When used in the nutritional creams, it has a great effect on preventing skin aging and wrinkles. When used in the food additives, the body is protected from damage caused by active oxygen. It can be of great help in preventing many diseases.

이하, 실시예 1과 실시예 2를 살펴보도록 한다. Hereinafter, let's look at Example 1 and Example 2.

[실시예 1] [Example 1]

실시예1의 제1파우더는 건조된 복분자 미숙과 1kg에 추출 용매로 사용된 물 10L를 혼합하여 제1조성물을 제조하고, 상기 제1조성물을 환류냉각장치를 부착한 히팅 맨틀을 이용하여 2시간 동안 2회 가열 추출하여 제1조성물 추출물을 제조하고, 상기 제1조성물 추출물을 여과지(ADVANTEC No. 2, Adventec Toyo Kaisha, Tokyo, Japan)를 이용하여 여과한 뒤 감압농축 후 동결건조하고 분쇄하여 제조된다. (이하,실시예1의 방법으로 제조된 제1파우더는 실시예1로 기재하기로 한다.)The first powder of Example 1 was prepared by mixing 1 kg of dried unripe bokbunja with 10 L of water used as an extraction solvent to prepare the first composition, and the first composition was heated for 2 hours using a heating mantle equipped with a reflux cooling device. An extract of the first composition was prepared by heat extraction twice, and the extract of the first composition was filtered using filter paper (ADVANTEC No. 2, Adventec Toyo Kaisha, Tokyo, Japan), concentrated under reduced pressure, freeze-dried, and pulverized. do. (Hereinafter, the first powder manufactured by the method of Example 1 will be described as Example 1.)

[실시예 2] [Example 2]

실시예2의 제1파우더는 건조된 복분자 미숙과 1kg에 추출 용매로 사용된 50% 에탄올 10L를 혼합하여 제1조성물을 제조하고, 상기 제1조성물을 환류냉각장치를 부착한 히팅 맨틀을 이용하여 2시간 동안 2회 가열 추출하여 제1조성물 추출물을 제조하고, 상기 제1조성물 추출물을 여과지(ADVANTEC No. 2, Adventec Toyo Kaisha, Tokyo, Japan)를 이용하여 여과한 뒤 감압농축 후 동결건조하고 분쇄하여 제조된다.(이하,실시예2의 방법으로 제조된 제1파우더는 실시예2로 기재하기로 한다.)The first powder of Example 2 was prepared by mixing 1 kg of dried unripe bokbunja with 10 L of 50% ethanol used as an extraction solvent to prepare the first composition, using a heating mantle equipped with a reflux cooling device. An extract of the first composition was prepared by heating extraction twice for 2 hours, and the extract of the first composition was filtered using filter paper (ADVANTEC No. 2, Adventec Toyo Kaisha, Tokyo, Japan), concentrated under reduced pressure, freeze-dried, and ground. (Hereinafter, the first powder manufactured by the method of Example 2 will be described as Example 2.)

이하, 용매가 각각 차별화되어 제조된 실시예 1과 실시예 2를 통한 구체적인 시험예를 살펴보도록 한다. Hereinafter, we will look at specific test examples through Examples 1 and 2 in which solvents were prepared differently.

[시험 방법] [Test Methods]

1. One. 세포배양cell culture

RAW 264.7 세포(mouse macrophage)는 American Type Culture Collection(ATCC, Manassas, VA, USA)에서 구입하였다. 세포배양을 위해 Dulbecco's modified Eagle's medium(DMEM, Gibco, Thermo Fisher Scientific, Waltham, MA, USA)에 56oC에서 30분간 열처리된 10%의 fetal bovine serum(FBS, Merck milliphore, Berlin, germany)과 1% 항생제(penicillin/streptomycin, Gibco)가 함유되게 첨가하여 95% 습도, 5% CO2, 37oC 세포 배양기에서 배양하였다.RAW 264.7 cells (mouse macrophage) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). For cell culture, 10% fetal bovine serum (FBS, Merck milliphore, Berlin, Germany) heat-treated at 56 o C for 30 minutes in Dulbecco's modified Eagle's medium (DMEM, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and 1 % antibiotics (penicillin/streptomycin, Gibco) were added and cultured in a cell incubator at 95% humidity, 5% CO 2 , and 37 o C.

2. 2. 세포생존율 측정Cell viability measurement

RAW 264.7 세포는 96-well microplate에 5×104 cells/mL 농도로 90 μL씩 분주하여 24시간 동안 배양한 뒤, 실시예1,실시예2를 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 μg/mL의 농도로 처리하고 24시간 배양하였다. 이후 cell viability assay kit(EZ-Cytox, DOGENbio, Seoul, Korea)를 이용하여 WST 용액을 10 μL씩 각 well에 분주한 뒤, 2시간 후 450 nm에서 흡광도를 측정하였다. RAW 264.7 cells were dispensed in 90 μL each at a concentration of 5×10 4 cells/mL in 96-well microplates and cultured for 24 hours, and then incubated in Examples 1 and 2 at 0, 31.25, 62.5, 125, 250, 500, Treated at concentrations of 1000 and 2000 μg/mL and cultured for 24 hours. Afterwards, 10 μL of WST solution was dispensed into each well using a cell viability assay kit (EZ-Cytox, DOGENbio, Seoul, Korea), and the absorbance was measured at 450 nm after 2 hours.

3. 3. Lactic dehydrogenase(LDH) 측정 Lactic dehydrogenase (LDH) measurement

RAW 264.7 세포를 1×105 cells/well의 농도로 24-well culture plate에 분주하였다. 24시간 배양 뒤 실시예1,실시예2로 24시간 동안 처리 후 배양배지의 원심분리를 통해 상등액을 획득하여 LDH cytotoxicity assay kit(EZ-LDH, DOGENBio) 분석을 시행하였다. LPS(Merck KGaA, St. Louis, MO, USA)는 500 μg/mL의 농도로 제1조성물 추출물을 처리 2시간 전에 전처리하였다. RAW 264.7 cells were distributed in a 24-well culture plate at a concentration of 1×10 5 cells/well. After culturing for 24 hours, the cells were treated with Examples 1 and 2 for 24 hours, and the supernatant was obtained through centrifugation of the culture medium and analyzed with an LDH cytotoxicity assay kit (EZ-LDH, DOGENBio). LPS (Merck KGaA, St. Louis, MO, USA) was pretreated at a concentration of 500 μg/mL 2 hours before treatment of the extract of the first composition.

4. 4. Intracellular ROS 측정Intracellular ROS measurements

RAW 264.7 세포는 5×104 cells/well의 농도로 96-well culture plate에 분주한 뒤 overnight 시켰다. LPS(500 μg/mL)를 처리하여 ROS를 유도하였고, 실시예1, 실시예2로 농도에 맞게 처리한 뒤 24시간 배양하였다. 이후 DCFH-DA(2', 7'-dichlorofluorescin-diacetate)를 첨가하고 30분 뒤에 형광값(Ex = 485/ Em = 525 nm)을 측정하였다. RAW 264.7 cells were seeded in a 96-well culture plate at a concentration of 5×10 4 cells/well and left overnight. ROS was induced by treatment with LPS (500 μg/mL), treated to the appropriate concentration in Examples 1 and 2, and cultured for 24 hours. Afterwards, DCFH-DA (2', 7'-dichlorofluorescin-diacetate) was added, and the fluorescence value (Ex = 485/ Em = 525 nm) was measured 30 minutes later.

5. 5. Nitric oxide(NO) 측정Nitric oxide (NO) measurement

RAW 264.7 cell을 1×105 cells/well의 농도로 24-well culture plate에 분주하였다. 24시간 배양 뒤 실시예1,실시예2로 24시간 동안 처리 후, 원심분리를 통해 상등액을 획득하여 NO 분석(Griess reagent assay kit, promega, Madison, Wisconsin, USA)을 수행하였다. LPS(500 μg/mL)는 제1조성물 추출물을 처리하기 2시간 전에 처리하였다. RAW 264.7 cells were distributed in a 24-well culture plate at a concentration of 1×10 5 cells/well. After culturing for 24 hours, the cells were treated with Examples 1 and 2 for 24 hours, and the supernatant was obtained through centrifugation and subjected to NO analysis (Griess reagent assay kit, promega, Madison, Wisconsin, USA). LPS (500 μg/mL) was treated 2 hours before treating the extract of the first composition.

6. 6. 염증성 사이토카인 분석Inflammatory cytokine analysis

RAW 264.7 세포를 1×105 cells/well의 농도로 24-well culture plate에 분주하고 overnight 시켰다. 실시예1, 실시예2로 처리하고 24시간 뒤 상등액을 획득하여 tumor necrosis factor(TNF-α), interleukin-1β(IL-1β) ELISA kit(Abcam, Cambridge, UK)을 이용하여 제조사에서 제공한 방법에 따라 분석을 진행하였다. RAW 264.7 cells were distributed in a 24-well culture plate at a concentration of 1×10 5 cells/well and left overnight. 24 hours after treatment in Example 1 and Example 2, the supernatant was obtained and used as a tumor necrosis factor (TNF-α), interleukin-1β (IL-1β) ELISA kit (Abcam, Cambridge, UK) provided by the manufacturer. Analysis was conducted according to the method.

7. 7. Quantitative RT PCRQuantitative RT PCR

RAW 264.7 세포를 6-well culture plate에 5×105 cells/well로 분주하고 overnight 시켰다. 이후 LPS를 처리하고 2시간 뒤 실시예1,실시예2를 처리하여 24시간 배양하였다. RNA 획득을 위해 RNA extraction kit(TaKaRa, MiniBEST Universal RNA Extraction Kit, Shiga, Japan)를 이용하여 제조사에서 제공한 방법을 토대로 RNA를 추출하였다. 추출된 RNA는 정량(BioSpec-nano, SHIMAZU, Kyoto, Japan)을 통해 total RNA 2 μg을 사용하여 cDNA를 합성(TaKaRa, RNA to cDNA EcoDry Premix)하였다. 합성된 cDNA를 이용하여 TNF-α, IL-1β, NF-κB, NF-κB inhibitor alpha(IκB-α), cyclooxygenase(COX)-2, inducible nitric oxygen synthase(iNOS), β-actin 유전자의 quantitative RT-PCR(Light Cycler 2.0, Roche, Mannheim, germany)을 수행하였다. 각각의 primer의 염기서열은 하기 표 1내지 표3과 같다. RAW 264.7 cells were distributed in 6-well plate culture at 5×10 5 cells/well and left overnight. Afterwards, LPS was treated, and 2 hours later, Examples 1 and 2 were treated and cultured for 24 hours. To obtain RNA, RNA was extracted using an RNA extraction kit (TaKaRa, MiniBEST Universal RNA Extraction Kit, Shiga, Japan) based on the method provided by the manufacturer. The extracted RNA was quantified (BioSpec-nano, SHIMAZU, Kyoto, Japan) and cDNA was synthesized (TaKaRa, RNA to cDNA EcoDry Premix) using 2 μg of total RNA. Quantitative analysis of TNF-α, IL-1β, NF-κB, NF-κB inhibitor alpha (IκB-α), cyclooxygenase (COX)-2, inducible nitric oxygen synthase (iNOS), and β-actin genes using synthesized cDNA. RT-PCR (Light Cycler 2.0, Roche, Mannheim, Germany) was performed. The base sequence of each primer is shown in Tables 1 to 3 below.

GeneGene Primer sequencePrimer sequence Product size(bp)Product size(bp) Tm (oC)Tm( oC ) TNF-αTNF-α SS AATGGCCTCCCTCTCATCAGTAATGGCCTCCTCTCATCAGT 8383 6262 ASAS GCTACAGGCTTGTCACTCGAATTGCTACAGGCTTGTCACTCGAATT IL-1βIL-1β SS CTCAATGGACAGAATATCAACCAACACTCAATGGACAGAATATCAACCAACA 123123 6060 ASAS ACAGGACAGGTATAGATTCTTTCCTTTGACAGGACAGGGTATAGATTCTTTCCTTTG NF-κBNF-κB SS GCACGGATGACAGAGGCGTGTATAAGGGCACGGATGACAGAGGGCGTGTATAAGG 420420 6262 ASAS GGCGGATGATCTCCTTCTCTCTGTCTGGGCGGATGATCTCCTTCTCTCTGTCTG

GeneGene Primer sequencePrimer sequence Product size(bp)Product size(bp) Tm (oC)Tm( oC ) IκB-αIκB-α SS TGCTGAGGCACTTCTGAGTGCTGAGGCACTTCTGAG 421421 5656 ASAS CTGTATCCGGGTGCTTGGCTGTATCCGGGTGCTTGG COX-2COX-2 SS GGGAAGCCTTCTCCAACCGGGAAGCTTCTCCAACC 245245 6060 ASAS GAACCCAGGTCCTCGCTTGAACCCAGGTCCTCGCTT iNOS iNOS SS CCTCCTCCACCCTACCAAGTCCTCCTCCACCCTACCAAGT 199199 6060 ASAS CACCCAAAGTGCTTCAGTCACACCCAAAGTGCTTCAGTCA

GeneGene Primer sequencePrimer sequence Product size(bp)Product size(bp) Tm (oC)Tm( oC ) β-actinβ-actin SS AGAGAAGCTGTGCTATGTTAGAGAAGCTGTGCTATGTT 79
79
 6060 ASAS CACAGGATTCCATACCCAAGCACAGGATTCCATACCCAAG

분석은 3회 반복을 시행하였고, 값은 평균 ± 표준오차로 나타내었다. 실험 결과는 SPSS(SPSS version 23.0, SPSS Inc, Chicago, IL, USA)를 이용하여 분석하였으며, Duncan`s multiple range test를 통해 P<0.05인 경우를 실시예1 또는 실시예2의 통계적 유의성이 있는 것으로 판단하였다.The analysis was repeated three times, and the values were expressed as mean ± standard error. The experimental results were analyzed using SPSS (SPSS version 23.0, SPSS Inc, Chicago, IL, USA). Through Duncan's multiple range test, P < 0.05 was considered statistically significant for Example 1 or Example 2. It was judged that it was.

[결과] [result]

1. One. 세포생존율 및 세포독성 Cell viability and cytotoxicity

실시예1과 실시예2에 대한 세포 생존율을 확인하였다. 복분자 미숙과 50% 에탄올 추출물을 처리한 실시예2가 물 추출물을 처리한 실시예 1에 비해 세포 생존율이 높은 경향을 나타내었다. 실시예1은 250 μg/mL의 농도까지 생존율 변화가 없었으나 500 μg/mL의 처리 농도에서부터 생존율 감소(11%)를 나타내었다. 실시예2 경우 500 μg/mL의 농도에서 실시예1과 비교했을 때 7%의 생존율 감소를 나타내었다. LPS에 의해 유도된 산화 스트레스로부터 실시예1,실시예2의 세포 보호 효과를 확인하기 위해 LDH를 측정한 결과, LPS를 처리한 그룹은 무처리 그룹에 비해 LDH 수치가 약 3.3배 증가되었고, 실시예1과 실시예2를 통해 처리하여 세포독성이 감소되었다. 실시예 1 또는 실시예 2로 처리했을 때, 두 그룹 모두 농도의존적인 LDH 감소를 나타내었으며, 실시예 2의 세포독성 저감 효과가 실시예 1 보다 약간 높은 경향을 보였으나 추출물 처리 농도에 따른 유의적 차이는 없어 세포독성 감소 및 세포 보호에 효과가 있다는 사실을 보여준다. Cell viability was confirmed for Examples 1 and 2. Example 2, which was treated with unripe bokbunja and 50% ethanol extract, tended to have a higher cell survival rate than Example 1, which was treated with water extract. Example 1 showed no change in survival rate up to a concentration of 250 μg/mL, but a decrease in survival rate (11%) from the treatment concentration of 500 μg/mL. In Example 2, the survival rate was reduced by 7% compared to Example 1 at a concentration of 500 μg/mL. As a result of measuring LDH to confirm the cell protection effect of Examples 1 and 2 from oxidative stress induced by LPS, the LDH level in the LPS-treated group increased by about 3.3 times compared to the untreated group. Cytotoxicity was reduced by processing through Example 1 and Example 2. When treated with Example 1 or Example 2, both groups showed a concentration-dependent decrease in LDH, and the cytotoxicity reduction effect of Example 2 tended to be slightly higher than that of Example 1, but there was a significant difference depending on the extract treatment concentration. There is no difference, showing that it is effective in reducing cytotoxicity and protecting cells.

2. 복분자 미숙과 추출물의 항산화 효과 2 . Antioxidant effect of unripe Bokbunja fruit extract

LPS는 전염증성 반응을 유발할 수 있는 ROS와 NO의 생성에 영향을 미친다 실시예2를 통해 LPS로 유도된 ROS와 NO에 미치는 영향을 확인하였다. 실시예1를 100 μg/mL의 농도로 처리했을 때 ROS의 생성은 LPS만 처리한 그룹에 비해 29% 억제되었으며, 실시예2를 100 μg/mL을 처리한 그룹은 44%의 억제 효과를 나타내었다. 따라서 ROS 억제 효과는 실시예2에서 더 높은 것으로 확인되었다. 또한, 실시예1과 실시예2를 100 μg/mL의 농도로 처리했을 때 약 30%의 NO 생성억제 효과를 나타내었다. 또한 NO 생성억제 효과 역시 실시예1 및 실시예2 모두에서 농도 의존적으로 감소됨을 확인할 수 있었다. LPS affects the production of ROS and NO, which can induce pro-inflammatory reactions. Through Example 2, the effect on ROS and NO induced by LPS was confirmed. When Example 1 was treated at a concentration of 100 μg/mL, the production of ROS was inhibited by 29% compared to the group treated with only LPS, and the group treated with Example 2 at 100 μg/mL showed an inhibition effect of 44%. It was. Therefore, the ROS inhibition effect was confirmed to be higher in Example 2. In addition, when Examples 1 and 2 were treated at a concentration of 100 μg/mL, the NO production inhibition effect was approximately 30%. In addition, it was confirmed that the NO production inhibition effect was also reduced in a concentration-dependent manner in both Examples 1 and 2.

3. 복분자 미숙과 추출물의 항염증 효과 3 . Anti-inflammatory effect of unripe Bokbunja fruit extract

LPS의 처리는 염증성 사이토카인 발현 증가를 통해 다양한 염증반응을 유도한다 실시예 1 및 실시예 2가 LPS로 유도된 염증성 사이토카인 중 TNF-α와 IL-1β에 미치는 변화를 세포배양액에 분비된 양과 mRNA의 발현을 통해 확인하였다. Treatment of LPS induces various inflammatory responses through increased expression of inflammatory cytokines. Examples 1 and 2 show changes in TNF-α and IL-1β among LPS-induced inflammatory cytokines in terms of the amounts secreted in the cell culture medium and the changes in IL-1β. This was confirmed through mRNA expression.

세포배양액에서 측정된 TNF-α의 수치는 LPS 단독 처리 그룹과 비교했을 때, 실시예1와 실시예2로 처리한 그룹에서 각각 27% 및 44%의 감소 효과를 나타내었다. 세포 내 TNF-α mRNA의 발현 역시 분비된 TNF-α 측정값과 유사한 비율(실시예1 : 27%, 50% 실시예2: 47%)로 감소되었음을 확인할 수 있었다. The level of TNF-α measured in the cell culture medium showed a decrease of 27% and 44% in the groups treated with Example 1 and Example 2, respectively, compared to the group treated with LPS alone. It was confirmed that the expression of intracellular TNF-α mRNA was also reduced at a rate similar to the measured value of secreted TNF-α (Example 1: 27%, 50%, Example 2: 47%).

또한 실시예2는 LPS만 처리한 Raw 264.7 세포에 비해 IL-1β의 분비가 9% 감소하였고, mRNA의 발현은 40% 감소하였다. 반면에 실시예1에서는 LPS 자극에 의한 IL-1β의 분비가 억제되는 경향을 보였으나 통계적 유의성은 없었고, 세포 내 IL-1β mRNA의 발현 역시 큰 변화가 없었다. Additionally, in Example 2, IL-1β secretion was reduced by 9% and mRNA expression was reduced by 40% compared to Raw 264.7 cells treated only with LPS. On the other hand, in Example 1, the secretion of IL-1β by LPS stimulation tended to be suppressed, but there was no statistical significance, and there was no significant change in the expression of intracellular IL-1β mRNA.

4.4. 복분자 미숙과 추출물의 NF-κB signaling pathway 조절 효과Effect of unripe Bokbunja fruit extract on regulating NF-κB signaling pathway

염증의 초기 반응은 주로 COX-2와 iNOS 및 이들에 의한 전염증성 매개물들에 의해 주도되며 여기에는 prostaglandin E2와 NO 등이 있다 NF-κB는 이러한 염증반응의 발생과 소실 단계에 관여함으로써 염증반응을 조절하는 중요한 역할을 하게 된다 실시예1 실시예2가 NF-κB 조절과 그 하위 인자에 미치는 영향에 대해 분석하였다. LPS의 처리로 증가된 NF-κB mRNA의 발현은 실시예 1 처리에 의해 15% 감소하였으며, 실시예2 처리로 28% 감소되었다 또한, NF-κB의 하위 조절인자인 COX-2와 iNOS mRNA 발현 역시 복분자 미숙과 추출물 처리에 의해 감소되었다. The initial response to inflammation is mainly driven by COX-2, iNOS, and their pro-inflammatory mediators, including prostaglandin E2 and NO. NF-κB initiates the inflammatory response by participating in the generation and disappearance stages of the inflammatory response. It plays an important role in regulating Example 1 and Example 2. The regulation of NF-κB and its effect on its subordinate factors were analyzed. The expression of NF-κB mRNA, which was increased by treatment with LPS, was decreased by 15% by treatment in Example 1 and by 28% by treatment in Example 2. Additionally, expression of COX-2 and iNOS mRNA, which are downstream regulators of NF-κB, It was also reduced by treatment with unripe bokbunja fruit extract.

실시예1은 COX-2와 iNOS mRNA 발현을 각각 16%와 9% 감소시켰고, 실시예2는 각각 32%와 16% 감소시켰다. 상기 결과로부터 LPS로 증가된 NF-κB와 그 하위 인자에 대한 억제 효과는 실시예2가 실시예1에 비해 높다는 것을 알 수 있다.Example 1 decreased COX-2 and iNOS mRNA expression by 16% and 9%, respectively, and Example 2 decreased COX-2 and iNOS mRNA expression by 32% and 16%, respectively. From the above results, it can be seen that the inhibitory effect on NF-κB and its subordinate factors increased by LPS in Example 2 is higher than that in Example 1.

결론적으로 복분자 미숙과 추출물을 활용한 실시예 1과 실시예 2는 항산화 및 항염증 효능을 갖는 기능성 식품 소재 또는 화장품으로 활용 가치가 있음을 알 수 있다. In conclusion, it can be seen that Examples 1 and 2 using unripe bokbunja fruit extracts are valuable as functional food materials or cosmetics with antioxidant and anti-inflammatory effects.

즉, 상기 실시예 1 또는 실시예2의 제1파우더는, 식품첨가제, 영양크림 중 선택되는 어느 1종으로 제조될 수 있다.That is, the first powder of Example 1 or Example 2 may be manufactured from any one selected from food additives and nutritional cream.

이하, 상기 실시예2를 통한 화장품 영양크림을 제조에 대해 구체적으로 살펴보도록 한다. Hereinafter, we will look in detail at the manufacturing of the cosmetic nutritional cream through Example 2.

영양크림은, 증류수(Water) 40 ~ 70 wt%, 글리세린(Glycerine) 10 ~ 40 wt%, 녹차씨오일(Cyclomethicone(DC345)) 5 ~ 7 wt%, 세티아릴 알코올(Cetearyl alcohol) 2 ~ 3 wt%, 포도씨유(Grape seed oil) 3 ~ 5 wt%, 소르비톨(Sorbitol) 1 ~ 2 wt%, 이소프로필 미리스테이트(Isopropyl myristate) 3 ~ 4 wt%, 디메치콘(Dimethiicone) 4 ~ 5 wt%의 혼합으로 조성되는 혼합물을 50 ~ 72 ℃에서 용해하여 제조되는 화장품 조성물; Nutritional cream contains 40 to 70 wt% distilled water, 10 to 40 wt% glycerine, 5 to 7 wt% green tea seed oil (Cyclomethicone (DC345)), and 2 to 3 wt% cetearyl alcohol. %, Grape seed oil 3 ~ 5 wt%, Sorbitol 1 ~ 2 wt%, Isopropyl myristate 3 ~ 4 wt%, Dimethicone 4 ~ 5 wt% A cosmetic composition prepared by dissolving the mixture formed by mixing at 50 to 72 ° C.;

복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 70 ~ 99.9 wt%를 혼합하여 제조된 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제1조성물 추출물을 여과하여 여과액을 제조한 후, 그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제조된 제1파우더;A filtrate is prepared by filtering the first composition extract prepared by heating and extracting the first composition prepared by mixing 0.01 to 30 wt% of unripe bokbunja and 70 to 99.9 wt% of 50% ethanol twice for 1 to 5 hours. After that, the filtrate was concentrated under reduced pressure, freeze-dried, and pulverized to produce a first powder;

화장품 조성물;에 제2파우더;를 첨가하여, 1,500 ~ 2,800 rpm에서 1 ~ 3시간 동안 교반하여 제조한다. It is prepared by adding a second powder to the cosmetic composition and stirring at 1,500 to 2,800 rpm for 1 to 3 hours.

이하, 화장품 영양크림을 제조의 각 부 구성에 대해 구체적으로 살펴보도록 한다. Hereinafter, we will look in detail at the composition of each part of manufacturing cosmetic nutritious cream.

[화장품 조성물][Cosmetic composition]

증류수(Water) 40 ~ 70 wt%, 글리세린(Glycerine) 10 ~ 40 wt%, 녹차씨오일(Cyclomethicone(DC345)) 5 ~ 7 wt%, 세티아릴 알코올(Cetearyl alcohol) 2 ~ 3 wt%, 포도씨유(Grape seed oil) 3 ~ 5 wt%, 소르비톨(Sorbitol) 1 ~ 2 wt%, 이소프로필 미리스테이트(Isopropyl myristate) 3 ~ 4 wt%, 디메치콘(Dimethiicone) 4 ~ 5 wt%의 혼합으로 조성되는 혼합물을 50 ~ 72 ℃에서 용해하여 제조한다. Distilled water 40 ~ 70 wt%, Glycerine 10 ~ 40 wt%, Green tea seed oil (Cyclomethicone (DC345)) 5 ~ 7 wt%, Cetearyl alcohol 2 ~ 3 wt%, Grapeseed oil It is composed of a mixture of 3 to 5 wt% of (Grape seed oil), 1 to 2 wt% of Sorbitol, 3 to 4 wt% of Isopropyl myristate, and 4 to 5 wt% of Dimethicone. The mixture is prepared by dissolving at 50 to 72°C.

더욱 구체적으로는, 증류수(Water) 70 wt%, 글리세린(Glycerine) 10 wt%, 녹차씨오일(Cyclomethicone(DC345)) 2 wt%, 세티아릴 알코올(Cetearyl alcohol) 3wt%, 포도씨유(Grape seed oil) 5 wt%, 소르비톨(Sorbitol) 1 wt%, 이소프로필 미리스테이트(Isopropyl myristate) 4 wt%, 디메치콘(Dimethiicone) 5 wt%의 혼합으로 조성되는 혼합물을 50 ~ 72 ℃에서 용해하여 제조한다. More specifically, distilled water 70 wt%, glycerine 10 wt%, green tea seed oil (Cyclomethicone (DC345)) 2 wt%, cetearyl alcohol 3 wt%, grape seed oil ) 5 wt%, 1 wt% sorbitol, 4 wt% isopropyl myristate, and 5 wt% dimethicone are prepared by dissolving at 50 to 72°C.

[제1파우더][No. 1 powder]

제1파우더는 복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 70 ~ 99.9 wt%를 혼합하여 제조된 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제1조성물 추출물을 여과하여 여과액을 제조한 후, 그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제조한다. The first powder is obtained by filtering the extract of the first composition prepared by mixing unripe bokbunja with 0.01 to 30 wt% and 70 to 99.9 wt% of 50% ethanol and heating and extracting the first composition twice for 1 to 5 hours. After preparing the filtrate, the filtrate is concentrated under reduced pressure, freeze-dried, and pulverized.

더욱 구체적으로는, 건조된 복분자 미숙과 10 wt%에 추출 용매로 사용된 50%의 에탄올 90 wt%을 혼합하여 제1조성물을 제조하고, 상기 제1조성물을 환류냉각장치를 부착한 히팅 맨틀을 이용하여 2시간 동안 2회 가열 추출하여 제1조성물 추출물을 제조하고, 상기 제1조성물 추출물을 여과지(ADVANTEC No. 2, Adventec Toyo Kaisha, Tokyo, Japan)를 이용하여 여과한 뒤 감압농축 후 동결건조하고 분쇄하여 제조된다. More specifically, a first composition was prepared by mixing 10 wt% of dried unripe bokbunja with 90 wt% of 50% ethanol used as an extraction solvent, and the first composition was mixed with a heating mantle equipped with a reflux cooling device. An extract of the first composition was prepared by heat extraction twice for 2 hours, and the extract of the first composition was filtered using filter paper (ADVANTEC No. 2, Adventec Toyo Kaisha, Tokyo, Japan), concentrated under reduced pressure, and then freeze-dried. It is manufactured by grinding.

상기 화장품 조성물 90 ~ 95 wt%;에 제2파우더 5 ~ 10 wt%;를 첨가하여, 1,500 ~ 2,800 rpm에서 1 ~ 3시간 동안 교반하여 제조한다. It is prepared by adding 5 to 10 wt% of the second powder to 90 to 95 wt% of the cosmetic composition and stirring at 1,500 to 2,800 rpm for 1 to 3 hours.

더욱 구체적으로는, 상기 화장품 조성물 95 wt%;에 제2파우더 5 wt%;를 첨가하여, 2,800 rpm에서 2시간 동안 교반하여 제조한다.More specifically, it is prepared by adding 5 wt% of the second powder to 95 wt% of the cosmetic composition and stirring at 2,800 rpm for 2 hours.

이하, 실시예 B에 따른 구체적인 내용을 살펴보도록 한다. Hereinafter, we will look at specific details according to Example B.

실시예 B는 실시예A를 베이스로 하되, 제2조성물 추출물과. 제3조성물 추출물를 더 포함하는 발명이다. Example B is based on Example A, but with an extract of the second composition. The invention further includes a third composition extract.

실시예 B는 복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 70 ~ 99.9 wt%를 혼합하여 제조된 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제1조성물 추출물과,Example B is a first composition extract prepared by heating and extracting the first composition prepared by mixing 0.01 to 30 wt% of unripe bokbunja and 70 to 99.9 wt% of 50% ethanol twice for 1 to 5 hours,

용안육 1 ~ 30 wt%, 산두근 1 ~ 30 wt%, 파극천 1 ~ 30 wt%, 파고지 1 ~ 30 wt%, 고량강 1 ~ 30 wt%, 필발 1 ~ 30 wt% 및 석곡 1 ~ 30 wt%을 혼합하여 제조된 제2조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제2조성물 추출물과. Yongan-yuk 1 ~ 30 wt%, Sangeun 1 ~ 30 wt%, Pageukcheon 1 ~ 30 wt%, Pagoji 1 ~ 30 wt%, Goryanggang 1 ~ 30 wt%, Pilbal 1 ~ 30 wt%, and Dendrobium 1 ~ 30 wt%. An extract of the second composition prepared by heating and extracting the second composition prepared by mixing twice for 1 to 5 hours.

매미애플 1 ~ 30 wt%, 플래토니아 1 ~ 30 wt%, 커스터드 사과 1 ~ 30 wt%, 자부티카바 1 ~ 30 wt%, 룰로 1 ~ 30 wt%를 혼합하여 제조된 제3조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제3조성물 추출물과,A third composition prepared by mixing 1 to 30 wt% of cicada apple, 1 to 30 wt% of plateonia, 1 to 30 wt% of custard apple, 1 to 30 wt% of jabuticaba, and 1 to 30 wt% of roulo was mixed in 1 to 3 wt%. An extract of the third composition prepared by heat extraction twice for 5 hours,

상기 제1조성물 추출물 80 ~ 90 wt%와, 제2조성물 추출물 1 ~ 10 wt%와, 제3조성물 추출물 1 ~ 10 wt%을 혼합하여 제조되는 최종추출물;을 여과하여 여과액을 제조한 후, 그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제조된 제2파우더인 것을 특징으로 한다. A final extract prepared by mixing 80 to 90 wt% of the first composition extract, 1 to 10 wt% of the second composition extract, and 1 to 10 wt% of the third composition extract; After filtering to prepare a filtrate, It is characterized as a second powder manufactured by concentrating the filtrate under reduced pressure, freeze-drying, and pulverizing the filtrate.

[제1조성물 추출물][First composition extract]

제1 조성물 추출물은 복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 70 ~ 99.9 wt%를 혼합하여 제조된 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조된다. The first composition extract is prepared by heating and extracting the first composition prepared by mixing 0.01 to 30 wt% of unripe Bokbunja and 70 to 99.9 wt% of 50% ethanol, twice for 1 to 5 hours.

[제2조성물 추출물][Extract of second composition]

제2조성물 추출물은 용안육 1 ~ 30 wt%, 산두근 1 ~ 30 wt%, 파극천 1 ~ 30 wt%, 파고지 1 ~ 30 wt%, 고량강 1 ~ 30 wt%, 필발 1 ~ 30 wt% 및 석곡 1 ~ 30 wt%을 혼합하여 제조된 제2조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조된다. The extract of the second composition includes 1 to 30 wt% of Yonganyuk, 1 to 30 wt% of Sangeun, 1 to 30 wt% of Pageukcheon, 1 to 30 wt% of Pagoji, 1 to 30 wt% of Goryanggang, 1 to 30 wt% of Pilbal, and Dendrobium. The second composition prepared by mixing 1 to 30 wt% is prepared by heating and extracting twice for 1 to 5 hours.

여기서, 용안육은 둥글고 모양이 용의 눈과 비슷하다 하여 용안육이라는 이름이 붙여졌다. 단맛을 내는 약재로 용안의 과실을 말린 것이다 구성 성분으로 비타민 A,비타민 B1,비타민 B2,비타민 B6,비타민 C,비타민 E를 포함하면서 피부개선에 큰 도움을 준다,Here, Yongan Yuk was given the name Yongan Yuk because it is round and similar in shape to a dragon's eye. It is a sweet-tasting medicinal herb made from dried longan fruit. It contains vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin C, and vitamin E, and is very helpful in improving skin.

상기 용안육이 1 wt% 미만이면 다양한 비타민을 포함하여 피부개선,건강증진 효과를 이룰 수 없을 수 있고, 또한 30 wt%를 초과하면 그에 따른 특별한 효과가 없어 경제적 손실이다. If the amount of Yongan meat is less than 1 wt%, skin improvement and health promotion effects including various vitamins may not be achieved, and if it exceeds 30 wt%, there is no special effect, resulting in an economic loss.

여기서, 산두근은 만년콩의 뿌리를 한방에서 이르는 말로서, 성질은 차고 맛은 쓰며, 독을 제거하고 통증을 가라앉히는 데에 쓰인다. 또한, 피부에 쌓인 노페물을 제거에 도움을 줄 수 있다. Here, Sangeun refers to the root of the perennial bean in oriental medicine. It is cold in nature and bitter in taste, and is used to remove poison and relieve pain. Additionally, it can help remove impurities accumulated on the skin.

상기 산두근이 1 wt% 미만이면 화장품으로 사용될 경우 피부에 쌓인 노폐물을 제거하기 힘들 수 있고, 식품첨가제로 사용될 경우 건강 증진 효과가 떨어질 수 있다. 또한, 30 wt%를 초과하면 그에 따른 특별한 효과가 없어 경제적 손실이다. If the amount of the chalazion is less than 1 wt%, it may be difficult to remove waste accumulated on the skin when used as a cosmetic, and when used as a food additive, the health promotion effect may be reduced. In addition, if it exceeds 30 wt%, there is no special effect and it is an economic loss.

여기서, 파극천은 파극천(巴戟天) Morinda officinalis How (꼭두서니과 Rubiaceae)의 뿌리로서 수염뿌리를 제거하고 납작하게 눌러서 말린 것이다. 또한, 다수의 비타민을 포함하고 아미노산이 함유되어 피부의 보습에 도움을 준다. Here, Pageukcheon is the root of Morinda officinalis How (Rubiaceae), which is dried by removing the beard root and pressing it flat. Additionally, it contains numerous vitamins and amino acids, which help moisturize the skin.

상기 파극천이 1 wt% 미만이면 비타민과 아미노산을 통한 보습효과가 떨어질 수 있고, 식품첨가제로 사용시 건강 증진효과가 떨어질 수 있다 또한, 30 wt%를 초과하면 과다 사용에 따른 부작용이 우려될 수 있어 한정 범위에서 사용하는 것이 바람직하다. If the above-mentioned Paeukcheon is less than 1 wt%, the moisturizing effect through vitamins and amino acids may be reduced, and the health-promoting effect may be reduced when used as a food additive. Additionally, if it exceeds 30 wt%, there may be concerns about side effects due to excessive use, so it is limited. It is desirable to use it within the range.

여기서, 파고지는 인도와 중국 전통 의학에서 사용되는 식물로서, 이 식물의 씨앗을 활용하는 것으로서, 상기 씨앗은 소랄렌을 비롯한 다양한 쿠마린이 들어 있고 피부의 독성을 제거해주데 도움을 줄 수 있다. Here, Pagoda is a plant used in Indian and Chinese traditional medicine, and the seeds of this plant are used. The seeds contain various coumarins, including psoralen, and can help remove toxicity from the skin.

상기 파고지가 1 wt% 미만이면 독성제거에 대한 효과가 미미할 수 있고, 식품첨가제로 사용시 건강 증진효과가 떨어질 수 있다If the pore size is less than 1 wt%, the effect on removing toxicity may be minimal, and the health promotion effect may be reduced when used as a food additive.

또한, 30 wt%를 초과하면 과다 사용에 따른 부작용이 우려될 수 있어 한정 범위에서 사용하는 것이 바람직하다. In addition, if it exceeds 30 wt%, there may be concerns about side effects due to excessive use, so it is desirable to use it within a limited range.

여기서, 고량강은 생강과에 속하는 식물이다. 양강이라 부르기도 한다. 씨는 홍두구로 부르며, 뿌리와 함께 한약재로 사용하는데 본발명에서는 뿌리만을 사용하는 것이고,항균작용한다. Here, Goryanggang is a plant belonging to the ginger family. It is also called Yanggang. The seeds are called red bean sprouts and are used together with the roots as an herbal medicine. In the present invention, only the roots are used, and they have an antibacterial effect.

상기 고령강이 1 wt% 미만이면 항균효과가 미미할 수 있고, 식품첨가제로 사용시 건강 증진효과가 떨어질 수 있다. If the amount of dried steel is less than 1 wt%, the antibacterial effect may be minimal, and the health promotion effect may be reduced when used as a food additive.

또한, 30 wt%를 초과하면 과다 사용에 따른 부작용이 우려될 수 있어 한정 범위에서 사용하는 것이 바람직하다. In addition, if it exceeds 30 wt%, there may be concerns about side effects due to excessive use, so it is desirable to use it within a limited range.

여기서, 필발은 후추과 식물인 Piper longum L의 채 익지 않은 열매이삭을 말린 것이다. 늦가을 누렇던 열매가 까맣게 될 때 따서 햇볕에 말린 것을 사용한다. 항균작용한다. Here, pilbal is dried unripe fruit spikes of Piper longum L, a plant in the pepper family. Use the ones picked in late fall when the yellow fruits turn black and dried in the sun. It has an antibacterial effect.

상기 필발이 1 wt% 미만이면 항균효과가 미미할 수 있고, 식품첨가제로 사용시 건강 증진효과가 떨어질 수 있다. If the amount of the required amount is less than 1 wt%, the antibacterial effect may be minimal, and the health promotion effect may be reduced when used as a food additive.

또한, 30 wt%를 초과하면 과다 사용에 따른 부작용이 우려될 수 있어 한정 범위에서 사용하는 것이 바람직하다. In addition, if it exceeds 30 wt%, there may be concerns about side effects due to excessive use, so it is desirable to use it within a limited range.

석곡은 바위나 죽은 나무 줄기에 붙어서 자란다. 뿌리줄기에서 굵은 뿌리가 많이 나온다. 줄기는 여러 개가 뭉쳐나고 곧게 서며 다육질이고 높이가 10∼20cm이다. 본 발명은 상기 줄기만을 사용하고 피부 노화방지에 도움을 줄 수 있다. Dendrobium grows attached to rocks or dead tree trunks. Many thick roots grow from the rhizome. Several stems grow in clusters, stand upright, are fleshy, and are 10 to 20 cm high. The present invention uses only the stem and can help prevent skin aging.

상기 석곡이 1 wt% 미만이면 피부 노화방지 효과가 미미할 수 있고, 식품첨가제로 사용시 건강 증진효과가 떨어질 수 있다. If the amount of dendrobium is less than 1 wt%, the anti-aging effect on the skin may be minimal, and the health promotion effect may be reduced when used as a food additive.

또한, 30 wt%를 초과하면 그에 따른 특별한 효과가 없으므로 무의미하다. Additionally, if it exceeds 30 wt%, it is meaningless because there is no special effect.

제2조성물 추출물은 용안육 20 wt%, 산두근 10 wt%, 파극천 1 wt%, 파고지 23 wt%, 고량강 25 wt%, 필발 1 wt% 및 석곡 20 wt%을 혼합하여 제조된 제2조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조된다. The extract of the second composition is a second composition prepared by mixing 20 wt% of longan meat, 10 wt% of sage root, 1 wt% of paprika, 23 wt% of pagoji, 25 wt% of goryanggang, 1 wt% of pilbal, and 20 wt% of dendrobium. It is prepared by heating and extracting twice for 1 to 5 hours.

본 발명에서의 제2조성물 추출물은 높은 항균작용과 보습효과로 항균과 보습을 이루는 효과를 갖는다. The extract of the second composition in the present invention has a high antibacterial and moisturizing effect.

또한, 식품첨가제로 사용시 상기 제시된 바와 같이 다양한 유효성분을 통한 약리 작용으로 건강 증진 효과를 이룰 수 있다. In addition, when used as a food additive, health promotion effects can be achieved through pharmacological action through various active ingredients as shown above.

[제3조성물 추출물][Extract of third composition]

제3조성물 추출물은 매미애플 1 ~ 30 wt%, 플래 토니아 1 ~ 30 wt%, 커스터드 사과 1 ~ 30 wt%, 자부티카바 1 ~ 30 wt%, 룰로 1 ~ 30 wt%를 혼합하여 제조된 제3조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조된다. The third composition extract is prepared by mixing 1 to 30 wt% of cicada apple, 1 to 30 wt% of plantonia, 1 to 30 wt% of custard apple, 1 to 30 wt% of jabuticaba, and 1 to 30 wt% of roulo. 3 It is prepared by heating and extracting the composition twice for 1 to 5 hours.

여기서, 매미애플 또는 Santo Domingo Apricot는 남아메리카 원산이면서 내부에는 연한 오렌지색에서 노란색 펄프가 있는 두꺼운 외부 껍질이 있다. 비타민 A,비타민 C, 리보플라빈등을 다수로 포함하여 피부 건강과 건강 증진 효과를 이룰 수 있다. Here, the cicada apple, or Santo Domingo Apricot, is native to South America and has a thick outer skin with a light orange to yellow pulp inside. It contains a lot of vitamin A, vitamin C, riboflavin, etc., so it can achieve skin health and health promotion effects.

매미애플이 1 wt% 미만이면 피부 건강 효과와 건강증진 효과가 미미할 수 있고, 30 wt%을 초과하면 설사나 다뇨가 일어날 가능성이 있어 한정범위에서 사용하는 것이 바람직하다. If cicada apple is less than 1 wt%, the skin health effect and health promotion effect may be minimal, and if it exceeds 30 wt%, diarrhea or polyuria may occur, so it is recommended to use it within a limited range.

여기서, 플래토니아(Platonia)는 바쿠리라고도 불리우며, 브라질과 파라과이의 열대 우림에서 발견되는 큰 나무 (40m에 도달)이다. 과일은 오렌지의 크기가 되며 눌렀을 때 노란색 라텍스가 스며 나오는 두꺼운 노란색 껍질이 있다. 또한, 높은 수준의 인, 철분 및 비타민 C를 함유하고 있다. Here, Platonia, also called bacuri, is a large tree (reaching 40 m) found in the tropical forests of Brazil and Paraguay. The fruit is about the size of an orange and has a thick yellow skin that exudes yellow latex when pressed. It also contains high levels of phosphorus, iron and vitamin C.

상기 플래토니아가 1 wt% 미만이면 피부 건강 효과와 건강 증진 효과가 미미할 수 있고, 30 wt%을 초과하면 그에 따른 특별한 효과가 없어 경제적 손실이다. If the plantonia is less than 1 wt%, the skin health effect and health promotion effect may be minimal, and if it exceeds 30 wt%, there is no special effect, resulting in an economic loss.

여기서, 커스터드 사과는 다른 이름 annona reticulata로서, 남미의 높은 산악 지역에서 발견되는 낙엽 식물이다. 과일은 막연하게 둥글다. 매우 향기롭고 수분이 많다. 커스터드 사과는 높은 수준의 비타민C와 항산화제로서, 기본적으로 피부 건강 증진에 뛰어나고 식품첨가제로 사용시 눈 건강 나아가 건강증진 효과에 좋으며, 식이섬유가 다량 포함되어 소화에 도움을 준다.Here, custard apple, by its other name, annona reticulata, is a deciduous plant found in the high mountainous regions of South America. The fruit is vaguely round. Very fragrant and juicy. Custard apples have a high level of vitamin C and antioxidants, and are basically excellent for promoting skin health. When used as a food additive, they are good for eye health and health promotion effects. They also contain a large amount of dietary fiber, which helps with digestion.

상기 커스타드 사과가 1 wt% 미만이면 피부 건강 효과와 건강 증진 효과가 미미할 수 있고, 30 wt%을 초과하면 그에 따라 타 성분의 함량이 줄어들어 한정범위로 사용하는 것이 바람직하다. If the custard apple is less than 1 wt%, the skin health effect and health promotion effect may be minimal, and if it exceeds 30 wt%, the content of other ingredients is reduced accordingly, so it is preferable to use it in a limited range.

여기서, 자부티카바(Plinia cauliflora)이 과일은 보통 브라질 포도 나무라고 불리며 브라질의 남동부 지방에서 자란다. 줄기에서 열매를 맺는다. 부드러운 젤라틴 살과 1-4 개의 검은 씨앗이 있다. 또한 폴리페놀 성분들이 다양하게 함유되고 플라보노이드계 성분, 안토시아닌 성분과 같은 갈산 성분들이 함유되어 피부 노호에 대한 도움과 피부건강 증진 효과에 뛰어나다, Here, Jabuticaba (Plinia cauliflora) This fruit is commonly called the Brazilian vine and grows in the southeastern region of Brazil. Fruits grow from stems. It has soft gelatinous flesh and 1-4 black seeds. In addition, it contains a variety of polyphenol ingredients and gallic acid ingredients such as flavonoids and anthocyanins, which are excellent for helping with skin aging and improving skin health.

상기 자부티가바 1 wt% 미만이면 피부 노화 방지 효과와 건강 증진 효과가 미미할 수 있고, 30 wt%을 초과하면 그에 따른 부작용이 나타날 수 있어 한정된 범위에서 사용하는 것이 바람직하다. If the amount of zabutigaba is less than 1 wt%, the skin aging prevention effect and health promotion effect may be minimal, and if it exceeds 30 wt%, side effects may occur, so it is preferable to use it in a limited range.

룰로는 룰로(lulo)라는 이름은 케추아 인디언어에서 유래됐으며 학명은 Solarium quitoense라고 하는데 키토(에콰도르 수도)의 밤그림자(솔라리움)라는 뜻이다. 작은 토마토 모양의 부드러운 과일로 먼 친척이다. The name lulo is derived from the Quechua Indian language, and its scientific name is Solarium quitoense, which means the night shadow (solarium) of Quito (the capital of Ecuador). It is a small tomato-shaped soft fruit and is a distant relative.

롤로는 카로틴과 토코페롤,비타민등 다양한 유효성분들을 포함하면서 피부에 대해 긍정적인 작용을 하고, 건강에도 도움을 줄 수 있다. Lolo contains various active ingredients such as carotene, tocopherol, and vitamins, which have positive effects on the skin and can be beneficial to health.

상기 자부티가바 1 wt% 미만이면 피부개선 효과가 미미할 수 있고, 30 wt%을 초과하면 그에 따른 특별한 효과가 없어 무의미하다. If the amount of zabutigaba is less than 1 wt%, the skin improvement effect may be minimal, and if it exceeds 30 wt%, there is no special effect and it is meaningless.

제3조성물 추출물은 매미애플 30 wt%, 플래 토니아 30 wt%, 커스터드 사과 20 wt%, 자부티카바 10 wt%, 룰로 10 wt%를 혼합하여 제조된 제3조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조된다. The extract of the third composition was prepared by mixing 30 wt% of cicada apple, 30 wt% of plantonia, 20 wt% of custard apple, 10 wt% of jabuticaba, and 10 wt% of Roulo twice for 1 to 5 hours. It is manufactured by heat extraction.

제3조성물을 포함하면서 높은수준에 비타민 C 비타민 B6을 포함하고 항산화 효과와 다양안 폴리페놀 화합물을 함유하여 피부노화방지에 도움을 줄 수 있다. In addition to the third composition, it contains high levels of vitamin C and vitamin B6, has antioxidant effects, and contains various polyphenol compounds, which can help prevent skin aging.

또한, 식품첨가제로서, 제3조성물을 포함하면 전번적인 건강 증진 효과를 이루어 건강기능식품으로 활용될 수 있다. In addition, as a food additive, including the third composition can achieve an overall health promotion effect and be used as a health functional food.

[최종추출물] [Final extract]

상기 제1조성물 추출물 80 ~ 90 wt%와, 제2조성물 추출물 1 ~ 10 wt%와, 제3조성물 추출물 1 ~ 10 wt%을 혼합하여 제조되는 최종추출물;을 여과하여 여과액을 제조한 후, 그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제2파우더를 제조한다. A final extract prepared by mixing 80 to 90 wt% of the first composition extract, 1 to 10 wt% of the second composition extract, and 1 to 10 wt% of the third composition extract; After filtering to prepare a filtrate, The filtrate is concentrated under reduced pressure, freeze-dried, and pulverized to produce a second powder.

더욱 구체적으로는 상기 제1조성물 추출물 90 wt%와, 제2조성물 추출물 5 wt%와, 제3조성물 추출물 5 wt%을 혼합하여 제조되는 최종추출물;을 여과하여 여과액을 제조한 후, 그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제2파우더를 제조한다. More specifically, the final extract prepared by mixing 90 wt% of the extract of the first composition, 5 wt% of the extract of the second composition, and 5 wt% of the extract of the third composition; filtered to prepare a filtrate, and then filtered. The liquid is concentrated under reduced pressure, freeze-dried, and pulverized to produce a second powder.

상기 제2파우더는, 식품첨가제 또는 영양크림 중 선택되는 어느 1종으로 제조될 수 있는 것을 특징으로 한다. The second powder is characterized in that it can be manufactured from any one selected from food additives or nutritional cream.

상기 영양크림은, 증류수(Water) 40 ~ 70 wt%, 글리세린(Glycerine) 10 ~ 40 wt%, 녹차씨오일(Cyclomethicone(DC345)) 5 ~ 7 wt%, 세티아릴 알코올(Cetearyl alcohol) 2 ~ 3 wt%, 포도씨유(Grape seed oil) 3 ~ 5 wt%, 소르비톨(Sorbitol) 1 ~ 2 wt%, 이소프로필 미리스테이트(Isopropyl myristate) 3 ~ 4 wt%, 디메치콘(Dimethiicone) 4 ~ 5 wt%의 혼합으로 조성되는 혼합물을 50 ~ 72 ℃에서 용해하여 제조되는 화장품 조성물; The nutritional cream contains 40 to 70 wt% of distilled water, 10 to 40 wt% of glycerine, 5 to 7 wt% of green tea seed oil (Cyclomethicone (DC345)), and 2 to 3 of cetearyl alcohol. wt%, Grape seed oil 3 ~ 5 wt%, Sorbitol 1 ~ 2 wt%, Isopropyl myristate 3 ~ 4 wt%, Dimethicone 4 ~ 5 wt% A cosmetic composition prepared by dissolving a mixture composed of a mixture at 50 to 72°C;

복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 70 ~ 99.9 wt%를 혼합하여 제조된 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제1조성물 추출물과,An extract of the first composition prepared by heating and extracting the first composition prepared by mixing 0.01 to 30 wt% of unripe Bokbunja and 70 to 99.9 wt% of 50% ethanol twice for 1 to 5 hours,

용안육 1 ~ 30 wt%, 산두근 1 ~ 30 wt%, 파극천 1 ~ 30 wt%, 파고지 1 ~ 30 wt%, 고량강 1 ~ 30 wt%, 필발 1 ~ 30 wt% 및 석곡 1 ~ 30 wt%을 혼합하고 상기 혼합물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제2조성물 추출물과. Yongan-yuk 1 ~ 30 wt%, Sangeun 1 ~ 30 wt%, Pageukcheon 1 ~ 30 wt%, Pagoji 1 ~ 30 wt%, Goryanggang 1 ~ 30 wt%, Pilbal 1 ~ 30 wt%, and Dendrobium 1 ~ 30 wt%. A second composition extract prepared by mixing and heating and extracting the mixture twice for 1 to 5 hours.

매미애플 1 ~ 30 wt%,플래 토니아 1 ~ 30 wt%, 커스터드 사과 1 ~ 30 wt%, 자부티카바 1 ~ 30 wt%, 룰로 1 ~ 30 wt%를 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제3조성물 추출물과,Cicada apple 1 to 30 wt%, plantonia 1 to 30 wt%, custard apple 1 to 30 wt%, jabuticaba 1 to 30 wt%, and roulo 1 to 30 wt% are heated and extracted twice for 1 to 5 hours. Extract of the third composition prepared,

상기 제1조성물 추출물과, 제2조성물 추출물과, 제3조성물 추출물을 혼합하여 제조되는 최종추출물;을 여과하여 여과액을 제조한 후, 그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제조된 제2파우더;The final extract prepared by mixing the extract of the first composition, the extract of the second composition, and the extract of the third composition; was filtered to prepare a filtrate, the filtrate was concentrated under reduced pressure, freeze-dried, and pulverized. second powder;

화장품 조성물;에 제2파우더;를 첨가하여, 1,500 ~ 2,800 rpm에서 1 ~ 3시간 동안 교반하하여 제조되는 것을 특징으로 한다. It is characterized in that it is prepared by adding a second powder to the cosmetic composition and stirring for 1 to 3 hours at 1,500 to 2,800 rpm.

이하. 실시예3에 따른 영양크림에 구체적인 시험예를 살펴보도록 한다. below. Let us look at a specific test example of the nutritional cream according to Example 3.

[실시예 3][Example 3]

증류수(Water) 70 wt%, 글리세린(Glycerine) 10 wt%, 녹차씨오일(Cyclomethicone(DC345)) 2 wt%, 세티아릴 알코올(Cetearyl alcohol) 3 wt%, 포도씨유(Grape seed oil) 5 wt%, 소르비톨(Sorbitol) 1 wt%, 이소프로필 미리스테이트(Isopropyl myristate) 4 wt%, 디메치콘(Dimethiicone) 5 wt%의 혼합으로 조성되는 혼합물을 50 ~ 72 ℃에서 용해하여 화장품 조성물을 제조한다. Distilled water 70 wt%, Glycerine 10 wt%, Green tea seed oil (Cyclomethicone (DC345)) 2 wt%, Cetearyl alcohol 3 wt%, Grape seed oil 5 wt% A cosmetic composition is prepared by dissolving a mixture of 1 wt% sorbitol, 4 wt% isopropyl myristate, and 5 wt% dimethicone at 50 to 72°C.

건조된 복분자 미숙과 10 wt%에 추출 용매로 사용된 50%의 에탄올 90 wt%을 혼합하여 제1조성물을 제조하고, 상기 제1조성물을 환류냉각장치를 부착한 히팅 맨틀을 이용하여 2시간 동안 2회 가열 추출하여 제1조성물 추출물을 제조하고,A first composition was prepared by mixing 10 wt% of dried unripe bokbunja with 90 wt% of 50% ethanol used as an extraction solvent, and the first composition was heated for 2 hours using a heating mantle equipped with a reflux cooling device. Preparing an extract of the first composition by heating extraction twice,

용안육 20 wt%, 산두근 10 wt%, 파극천 1 wt%, 파고지 23 wt%, 고량강 25 wt%, 필발 1 wt% 및 석곡 20 wt%을 혼합하여 제조된 제2조성물을 환류냉각장치를 부착한 히팅 맨틀을 이용하여 2시간 동안 2회 가열 추출하여 제2조성물 추출물을 제조하고, The second composition prepared by mixing 20 wt% of yongan meat, 10 wt% of sorghum root, 1 wt% of paprika, 23 wt% of pagoji, 25 wt% of goryanggang, 1 wt% of pilbal, and 20 wt% of dendrite was attached to a reflux cooling device. An extract of the second composition was prepared by heating and extracting twice for 2 hours using one heating mantle,

매미애플 30 wt%, 플래 토니아 30 wt%, 커스터드 사과 20 wt%, 자부티카바 10 wt%, 룰로 10 wt%를 혼합하여 제조된 제3조성물을 환류냉각장치를 부착한 히팅 맨틀을 이용하여 2시간 동안 2회 가열 추출하여 제3조성물 추출물을 제조하고,The third composition prepared by mixing 30 wt% of cicada apple, 30 wt% of planonia, 20 wt% of custard apple, 10 wt% of jabuticaba, and 10 wt% of roulo was mixed using a heating mantle equipped with a reflux cooling device. Preparing a third composition extract by heating and extracting twice over a period of time,

상기 제1조성물 추출물 90 wt%와, 제2조성물 추출물 5 wt%와, 제3조성물 추출물 5 wt%을 혼합하여 제조되는 최종추출물;을 여과하여 여과액을 제조한 후, 그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제2파우더를 제조한다. A final extract prepared by mixing 90 wt% of the first composition extract, 5 wt% of the second composition extract, and 5 wt% of the third composition extract; filtering to prepare a filtrate, and then concentrating the filtrate under reduced pressure. After being freeze-dried and pulverized, a second powder is produced.

상기 화장품 조성물 80 wt%;에 제2파우더 20 wt%;를 첨가하여, 2,800 rpm에서 2시간 동안 교반하하여 실시예3의 영양크림을 제조한다.The nutritional cream of Example 3 was prepared by adding 20 wt% of the second powder to 80 wt% of the cosmetic composition and stirring at 2,800 rpm for 2 hours.

1.[시험예]1.[Test example]

[Collagenase 저해율 측정][Collagenase inhibition rate measurement]

콜라겐을 분해하는 콜라겐분해효소의 작용을 이용하여 활성 정도를 평가하는 것으로 50% 활성이 감소하는 농도를 IC50(㎍/㎖)로 저해 정도를 측정하였다.By evaluating the degree of activity using the action of collagenase, which decomposes collagen, the degree of inhibition was measured using IC 50 (㎍/ml), which is the concentration at which activity is reduced by 50%.

콜라겐을 30 mM Tris-HCl(pH 8.5)와 8 mM 염화 칼슘을 충액에 녹여 3 ㎍/㎖의 기질액을 만든다. 완충액 215 ㎕, 기질액 215 ㎕, 실시예 3을 500 ㎍/㎖로 희석시킨 시료용액 22.5 ㎕를 첨가한 후 콜라겐분해효소를 첨가하여 35 ℃에서 2시간 반응 시킨다. 1.89 % 니히드린 시약 98.7 ㎕를 더하여 18분간 반응 한 후 ELISA reader로 560 nm에서 흡광도를 측정하였다. Collagen was dissolved in 30mM Tris-HCl (pH 8.5) and 8mM calcium chloride to make a 3㎍/㎖ matrix solution. After adding 215 ㎕ of buffer solution, 215 ㎕ of substrate solution, and 22.5 ㎕ of sample solution diluted to 500 ㎍/㎖ of Example 3, collagenase was added and reacted at 35°C for 2 hours. After adding 98.7 ㎕ of 1.89% nihydrin reagent and reacting for 18 minutes, the absorbance was measured at 560 nm with an ELISA reader.

측정결과 실시예 3의 저해율은 81.12 % 로 확인되었다. 콜라겐분해효소 저해율 결과를 통해 주름개선 효과가 있음을 확인하였으며, 이로써 본 발명의 제2파우더가 영양크림으로도 유의적인 효과를 나타내는 것을 알 수 있다. As a result of the measurement, the inhibition rate of Example 3 was confirmed to be 81.12%. It was confirmed that there was a wrinkle improvement effect through the collagenase inhibition rate results, and it can be seen that the second powder of the present invention has a significant effect even as a nutritional cream.

2. [시험예]2. [Test example]

40 ~ 50세의 중년 여성층을 상대로 정상, 지성, 건성 피부를 가진 40명을 각각 25%씩으로 구성했다. 실시예 3 및 비교 예 1(통상적인 방법으로 제조된 수분크림)을 피부에 적용하게 하였다. 동일인에게 안면 위쪽 부분에는 실시예 3의 영양크림을, 안면 오른쪽 부분에는 비교예 1의 영양크림을 매일 저녘 세안 후 피부에 1일 1회 20일간 도포하게 함으로서 측정했다. Among middle-aged women aged 40 to 50, 40 people with normal, oily, and dry skin made up 25% each. Example 3 and Comparative Example 1 (moisturizing cream prepared by a conventional method) were applied to the skin. Measurements were taken by having the same person apply the nutritional cream of Example 3 to the upper part of the face and the nutritional cream of Comparative Example 1 to the right part of the face once a day for 20 days after washing the face every evening.

또한, 피부의 수분 유지를 가장 중요하게 두어 평가하도록 하였고, 평가 등급은 '매우 좋다', '좋다', '보통이다', '효과가 전혀 없다' 의 4가지로 분류하여 그 결과를 표 4 및 표 5에 나타내었다.In addition, the skin's moisture retention was evaluated as the most important, and the evaluation grades were classified into four categories: 'very good', 'good', 'average', and 'no effect at all', and the results are shown in Table 4 and It is shown in Table 5.


구분
division 피부상태 개선Improved skin condition 인원수Number of people %%

실시 예 3

Example 3
 매우 좋다very good 88 4040 좋다good night 77 3535 보통이다is average 55 2525 효과가 전혀 없다no effect at all 00 00


비교 예 1
Comparison example 1


 매우 좋다very good 22 1010 좋다good night 33 1515 보통이다is average 99 4545 효과가 전혀 없다no effect at all 66 3030

실시예3은 75% 이상에서 피부 개선 효과가 우수한 것으로 평가되었지만, 일반적인 방법으로 제조된 비교예 1의 경우에는 75% 이상이 별다른 피부 개선 효과를 얻지 못한다고 답하였다. Example 3 was evaluated as having an excellent skin improvement effect in more than 75% of cases, but in the case of Comparative Example 1 prepared by a general method, more than 75% responded that no significant skin improvement effect was obtained.

본 발명은 화장품 영양 크림, 식품첨가제등 다양한 용도로 활용될 수 있는 만능 첨가제로 화장품 산업, 식품 산업등 여러 분야에서 돌풍을 일으킬 것이라 예상되는 바 산업산 이용 가능성이 매우 높다. The present invention is an all-purpose additive that can be used for various purposes such as cosmetic nutritional cream and food additives, and is expected to create a sensation in various fields such as the cosmetics industry and the food industry, so the possibility of industrial use is very high.

Claims (6)

건조된 복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 또는 물 중 선택되는 어느 1종인 추출용매 70 ~ 99.9 wt%를 혼합하여 제1조성물을 제조하고,
상기 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제1조성물 추출물을 제조하고, 상기 제1조성물 추출물을 여과하여 여과액을 제조한 후,
그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제조된 제1파우더인 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 파우더.
A first composition is prepared by mixing dried unripe bokbunja with 0.01 to 30 wt% and 70 to 99.9 wt% of an extraction solvent selected from 50% ethanol or water,
The first composition was heated and extracted twice for 1 to 5 hours to prepare a first composition extract, and the first composition extract was filtered to prepare a filtrate,
A powder containing unripe Bokbunja fruit extract, characterized in that it is the first powder manufactured by concentrating the filtrate under reduced pressure, freeze-drying, and pulverizing the filtrate.
 청구항 1항에 있어서,
상기 제1파우더는,
식품첨가제, 영양크림 중 선택되는 어느 1종으로 제조될 수 있는 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 파우더.
In claim 1,
The first powder is,
A powder containing unripe bokbunja fruit extract, which can be manufactured with any one selected from food additives and nutritional cream.
 복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 70 ~ 99.9 wt%를 혼합하여 제조된 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제1조성물 추출물과,
용안육 1 ~ 30 wt%, 산두근 1 ~ 30 wt%, 파극천 1 ~ 30 wt%, 파고지 1 ~ 30 wt%, 고량강 1 ~ 30 wt%, 필발 1 ~ 30 wt% 및 석곡 1 ~ 30 wt%을 혼합하여 제조된 제2조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제2조성물 추출물과.
매미애플 1 ~ 30 wt%, 플래토니아 1 ~ 30 wt%, 커스터드 사과 1 ~ 30 wt%, 자부티카바 1 ~ 30 wt%, 룰로 1 ~ 30 wt%를 혼합하여 제조된 제3조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제3조성물 추출물과,
상기 제1조성물 추출물, 제2조성물 추출물 및 제3조성물 추출물을 혼합하여 제조되는 최종추출물;을 여과하여 여과액을 제조한 후, 그 여과액을 감압농축하고 동결건조한 후, 분쇄하여 제조된 제2파우더인 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 파우더.
An extract of the first composition prepared by heating and extracting the first composition prepared by mixing 0.01 to 30 wt% of unripe Bokbunja and 70 to 99.9 wt% of 50% ethanol twice for 1 to 5 hours,
Yongan-yuk 1 ~ 30 wt%, Sangeun 1 ~ 30 wt%, Pageukcheon 1 ~ 30 wt%, Pagoji 1 ~ 30 wt%, Goryanggang 1 ~ 30 wt%, Pilbal 1 ~ 30 wt%, and Dendrobium 1 ~ 30 wt%. A second composition extract prepared by heating and extracting the second composition prepared by mixing the second composition twice for 1 to 5 hours.
A third composition prepared by mixing 1 to 30 wt% of cicada apple, 1 to 30 wt% of plateonia, 1 to 30 wt% of custard apple, 1 to 30 wt% of jabuticaba, and 1 to 30 wt% of roulo was mixed in 1 to 3 wt%. An extract of the third composition prepared by heat extraction twice for 5 hours,
The final extract prepared by mixing the extract of the first composition, the extract of the second composition, and the extract of the third composition; was filtered to prepare a filtrate, and then the filtrate was concentrated under reduced pressure, freeze-dried, and pulverized. A powder containing unripe Bokbunja fruit extract, characterized in that it is a powder.
 청구항 2항에 있어서,
상기 제2파우더는,
식품첨가제 또는 영양크림 중 선택되는 어느 1종으로 제조될 수 있는 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 파우더.
In claim 2,
The second powder is,
A powder containing unripe Bokbunja fruit extract, which can be manufactured with any one selected from food additives or nutritional cream.
 청구항 4항에 있어서,
영양크림은,
복분자 미숙과 0.01 ~ 30 wt% 및 50%의 에탄올 70 ~ 99.9 wt%를 혼합하여 제조된 제1조성물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제1조성물 추출물과,
용안육 1 ~ 30 wt%, 산두근 1 ~ 30 wt%, 파극천 1 ~ 30 wt%, 파고지 1 ~ 30 wt%, 고량강 1 ~ 30 wt%, 필발 1 ~ 30 wt% 및 석곡 1 ~ 30 wt%을 혼합하고 상기 혼합물을 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제2조성물 추출물과.
매미애플 1 ~ 30 wt%,플래 토니아 1 ~ 30 wt%, 커스터드 사과 1 ~ 30 wt%, 자부티카바 1 ~ 30 wt%, 룰로 1 ~ 30 wt%를 1 ~ 5 시간 동안 2회 가열 추출하여 제조되는 제3조성물 추출물과,
상기 제1조성물 추출물과, 제2조성물 추출물과, 제3조성물 추출물을 혼합하여 제조되는 최종추출물;을 여과하여 여과액을 제조한 후, 그 여과액을 감압 농축하고 동결건조한 후, 분쇄하여 제조되는 제2파우더;에,
증류수(Water) 40 ~ 70 wt%, 글리세린(Glycerine) 10 ~ 40 wt%, 녹차씨오일(Cyclomethicone(DC345)) 5 ~ 7 wt%, 세티아릴 알코올(Cetearyl alcohol) 2 ~ 3 wt%, 포도씨유(Grape seed oil) 3 ~ 5 wt%, 소르비톨(Sorbitol) 1 ~ 2 wt%, 이소프로필 미리스테이트(Isopropyl myristate) 3 ~ 4 wt%, 디메치콘(Dimethiicone) 4 ~ 5 wt%의 혼합으로 조성되는 혼합물을 50 ~ 72 ℃에서 용해하여 제조되는 화장품 조성물;를 더 첨가한 후, 1,500 ~ 2,800 rpm에서 1 ~ 3 시간 동안 교반하여 제조되는 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 파우더.
In claim 4,
Nutritional cream,
An extract of the first composition prepared by heating and extracting the first composition prepared by mixing 0.01 to 30 wt% of unripe Bokbunja and 70 to 99.9 wt% of 50% ethanol twice for 1 to 5 hours,
Yongan-yuk 1 ~ 30 wt%, Sangeun 1 ~ 30 wt%, Pageukcheon 1 ~ 30 wt%, Pagoji 1 ~ 30 wt%, Goryanggang 1 ~ 30 wt%, Pilbal 1 ~ 30 wt%, and Dendrobium 1 ~ 30 wt%. A second composition extract prepared by mixing and heating and extracting the mixture twice for 1 to 5 hours.
Cicada apple 1 to 30 wt%, plantonia 1 to 30 wt%, custard apple 1 to 30 wt%, jabuticaba 1 to 30 wt%, and roulo 1 to 30 wt% are heated and extracted twice for 1 to 5 hours. An extract of the third composition prepared,
A final extract prepared by mixing the extract of the first composition, the extract of the second composition, and the extract of the third composition; is filtered to prepare a filtrate, the filtrate is concentrated under reduced pressure, freeze-dried, and pulverized. 2nd powder;
Distilled water 40 ~ 70 wt%, Glycerine 10 ~ 40 wt%, Green tea seed oil (Cyclomethicone (DC345)) 5 ~ 7 wt%, Cetearyl alcohol 2 ~ 3 wt%, Grapeseed oil It is composed of a mixture of 3 to 5 wt% of (Grape seed oil), 1 to 2 wt% of Sorbitol, 3 to 4 wt% of Isopropyl myristate, and 4 to 5 wt% of Dimethicone. A cosmetic composition prepared by dissolving the mixture at 50 to 72 ℃; A powder containing unripe bokbunja fruit extract, characterized in that it is prepared by adding more and stirring at 1,500 to 2,800 rpm for 1 to 3 hours.
 청구항 4항에 있어서
식품첨가제는,
제2파우더 1 ~ 10 wt%에 물 90 ~ 99 wt%을 더 혼합하여 제조되는 것을 특징으로 하는 복분자 미숙과 추출물을 포함하는 파우더.











In claim 4
Food additives,
A powder containing unripe Bokbunja fruit extract, which is manufactured by further mixing 90 to 99 wt% of water with 1 to 10 wt% of the second powder.
KR1020220142276A 2022-10-31 2022-10-31 Multi-Purpose Powder Containing Unripe Bokbunja Extract KR20240063263A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130104205A (en) 2012-03-13 2013-09-25 재단법인 고창복분자연구소 Tea comprising rubus coreanus miquel immature fruit and making method thereof
KR20150116569A (en) 2014-04-08 2015-10-16 전라북도 고창군 Tablet containing extract of unripened black raspberry and manufacturing method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130104205A (en) 2012-03-13 2013-09-25 재단법인 고창복분자연구소 Tea comprising rubus coreanus miquel immature fruit and making method thereof
KR20150116569A (en) 2014-04-08 2015-10-16 전라북도 고창군 Tablet containing extract of unripened black raspberry and manufacturing method thereof

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