KR20240015492A - Cosmetic composition for skin improvement that shows antibacterial activity and antibacterial effect using a mixed extract of Distromium decumbens, Rosa rugosaThunb., Persicaria hydropiper L. - Google Patents
Cosmetic composition for skin improvement that shows antibacterial activity and antibacterial effect using a mixed extract of Distromium decumbens, Rosa rugosaThunb., Persicaria hydropiper L. Download PDFInfo
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- KR20240015492A KR20240015492A KR1020220093444A KR20220093444A KR20240015492A KR 20240015492 A KR20240015492 A KR 20240015492A KR 1020220093444 A KR1020220093444 A KR 1020220093444A KR 20220093444 A KR20220093444 A KR 20220093444A KR 20240015492 A KR20240015492 A KR 20240015492A
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- mixed extract
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9711—Phaeophycota or Phaeophyta [brown algae], e.g. Fucus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/591—Mixtures of compounds not provided for by any of the codes A61K2800/592 - A61K2800/596
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
본 발명은 두켜부채, 해당화, 여뀌의 혼합 추출물을 포함하는 항산화, 항염 효과가 우수한 화장품 조성물에 관한 것이다.
본 발명의 화장료 조성물은 두켜부채, 해당화 및 여뀌의 알칼리 이온수 저온 침출 혼합 추출물을 전체 중량에 대하여 0.01~10 중량% 함유하는 것을 특징으로 한다.The present invention relates to a cosmetic composition with excellent antioxidant and anti-inflammatory effects, comprising mixed extracts of Dukwichae, Gyeonghwa, and Yeokwi.
The cosmetic composition of the present invention is characterized in that it contains 0.01 to 10% by weight of the mixed extract of alkaline ionized water and low-temperature leaching of Dukwichae, Gonghwa and Yeokwi based on the total weight.
Description
본 발명은 두켜부채, 해당화, 여뀌의 혼합 추출물을 포함하는 항산화, 항염 효과가 우수한 화장품 조성물에 관한 것이다 The present invention relates to a cosmetic composition with excellent antioxidant and anti-inflammatory effects, comprising mixed extracts of Dukwichae, Gyeonghwa, and Yeokwi.
피부노화를 설명하기에 적합한 노화이론은 환경기인설(environmental etiology)이다. 그 중 자외선과 호흡을 통해 생성되는 활성산소(free-radical)는 피부노화에 가장 중요한 요인으로 간주되고 있다. 이렇듯 활성산소가 피부노화의 주요 원인으로 밝혀짐에 따라 활성산소를 제거하는 항산화제, 즉 항산화 비타민의 체내흡수가 피부노화를 예방하고 지연시키는 데에 중요한 연구과제가 되고 있다. 하지만 피부미용학적 연구에서는 피부노화를 예방하고 지연시키는 측면이 화장품을 통해 항산화 비타민을 흡수케 하거나 매뉴얼 테크닉을 이용한 외양적인 변화에만 목적을 두고 있는 실정이다. 유용성을 가지고 있는 화장품과 매뉴얼 테크닉은 피부미용학에서 필요한 항목이다. 그러나 피부 노화를 예방하고 지연시키는 측면이 화장품과 매뉴얼 테크닉으로만 해결하려 한다면 곧이어 한계에 부딪치게 될 것이다. 피부 노화란 생체 산화에 따른 외부변화 현상이기 때문이다. 따라서 피부의 아름다움을 유지하고 관리하는 피부미용학적 연구에서도 피부의 외양적 관리와 함께 항산화 비타민의 체내 흡수도 병행되어야 할 것인데, 그러기 위해서는 항산화 비타민의 체내 흡수를 통한 피부의 변화 양상에 대한 지속적인 연구가 이루어져야 할 것이다. 이러한 연구가 이루어질 때에 피부미용학의 범위가 넓어질 것이며, 고객에 대한 전인적 관리도 기대할 수 있을 것이다.An aging theory suitable to explain skin aging is environmental etiology. Among them, free radicals generated through ultraviolet rays and respiration are considered the most important factors in skin aging. As free radicals have been found to be the main cause of skin aging, the absorption of antioxidants that remove free radicals, that is, antioxidant vitamins, into the body has become an important research topic in preventing and delaying skin aging. However, in skin aesthetic research, preventing and delaying skin aging is aimed only at absorbing antioxidant vitamins through cosmetics or changing appearance using manual techniques. Cosmetics and manual techniques that have utility are necessary items in dermatology. However, if you try to solve the problem of preventing and delaying skin aging only with cosmetics and manual techniques, you will soon run into limitations. This is because skin aging is an external change phenomenon caused by biological oxidation. Therefore, in aesthetic research to maintain and manage the beauty of the skin, the absorption of antioxidant vitamins into the body should be carried out in parallel with the external management of the skin. To do this, continuous research on the changes in the skin through the absorption of antioxidant vitamins into the body is required. It will have to be done. When such research is conducted, the scope of skin aesthetics will expand, and holistic care for customers will also be expected.
사람의 피부는 진피(dermis)와 표피(epidermis)로 구성되고 표피는 지질을 주로 생성하고, 콜라겐은 실질적으로 합성하지 않는 각질형성세포(keratinocyte)로 주로 구성되어 있어 특히, 피부의 최외각에 위치하고 있는 표피는 외부의 다양한 물리적, 화학적 및 기계적 자극에 대한 방어와 피부를 통한 체내 수분의 과도한 발산을 막는 보호 기능을 수행하고 각질형성세포로 구성된 각질층이 정상적으로 형성되고 유지됨으로써 가능하다. 각질형성세포는 표피 최하층(stratum basale)에서 지속적으로 증식하던 기저세포(basal cell)가 각질층(stratum corneum)으로 이동하면서, 단계적으로 형태 및 기능상의 변화를 거치며 형성된 세포이며, 일정 기간이 지나면 오래된 각질형성세포는 피부에서 탈락되고, 표피 최하층으로부터 올라온 새로운 각질형성세포가 그 기능을 대신하는 표피분화(epidermis differentiation) 또는 각화(keratinization)의 과정을 반복하게 되며 각질형성 세포는 천연보습인자(natural moisturizing factor, NMF)와 세라마이드, 콜레스테롤 및 지방산과 같은 세포 간 지질을 생성하여, 각질층이 외부와의 차단층 역할을 하게 됨으로써 피부장벽(skin barrier)로서의 기능을 보유하게 된다. 피부장벽(skin barrier)은 지속적인 외부 자극에 의해 손상되어 피부건조증, 아토피 등의 피부 질환이 유발되는데 현재 화장품에서 손상된 장벽을 보충하기 위해 보습제, 세라마이드 등 증상완화를 위한 물질을 사용할 뿐 피부 항상성 기전에 근거한 근본적인 효능 물질 개발은 미미한 상황이다.Human skin is composed of the dermis and epidermis. The epidermis mainly produces lipids, and is mainly composed of keratinocytes, which do not actually synthesize collagen. In particular, it is located in the outermost layer of the skin. The epidermis performs a protective function to defend against various external physical, chemical and mechanical stimuli and prevent excessive dissipation of moisture in the body through the skin, and this is possible by the normal formation and maintenance of the stratum corneum composed of keratinocytes. Keratinocytes are cells formed through gradual changes in form and function as basal cells, which continuously proliferate in the lowest layer of the epidermis (stratum basale), move to the stratum corneum, and after a certain period of time, old keratin cells become old. The keratinocytes are eliminated from the skin, and the process of epidermis differentiation or keratinization is repeated, with new keratinocytes rising from the lowest layer of the epidermis taking over their function. The keratinocytes produce natural moisturizing factors. , NMF) and intercellular lipids such as ceramide, cholesterol, and fatty acids are produced, and the stratum corneum functions as a skin barrier by acting as a barrier to the outside. The skin barrier is damaged by continuous external stimulation, causing skin diseases such as dry skin and atopy. Currently, cosmetics only use substances to relieve symptoms such as moisturizers and ceramides to replenish the damaged barrier, but they do not affect the skin homeostasis mechanism. The development of fundamental effective substances based on efficacy is minimal.
신체의 활성산소는 노화를 가속시키는데 몸 내부의 노화 뿐만 아니라 피부의 활성산소를 없애는 항산화 효과가 중요하다. 유해산소로도 불리는 활성산소는 환경오염과 화학물질, 자외선, 혈액순환장애, 스트레스 등으로 산소가 과잉 생산되며 만들어지는 것으로 신체에서 산화작용을 일으킨다. 이런 활성산소는 산화작용을 하며 세포 구조나 신호 전달 체계에 이상을 발생시키고 피부를 칙칙하게 만들 뿐 아니라 노화를 가속화하는 주범이다. 피부 건강을 되찾기 위해서 활성산소를 억제해야 한다. 이 때문에 피부에 좋지 않은 각종 유해산소를 산화하는 항산화 효과를 보이는 유효성분이 들어가는 화장료 조성물이 필요하다.Free radicals in the body accelerate aging, and the antioxidant effect that eliminates free radicals on the skin as well as aging inside the body is important. Active oxygen, also called harmful oxygen, is created when oxygen is excessively produced due to environmental pollution, chemicals, ultraviolet rays, blood circulation disorders, stress, etc., and causes oxidation in the body. These free radicals act as oxidizers and are the main culprit in causing abnormalities in cell structure and signaling systems, making skin dull, and accelerating aging. To restore skin health, free radicals must be suppressed. For this reason, there is a need for a cosmetic composition containing active ingredients that have an antioxidant effect that oxidizes various harmful oxygen species that are harmful to the skin.
또한 미국립게놈연구소 연구팀이 '사이언스저널'에 밝힌 연구결과에 의하면 피부에는 약 500~1000종 가량의 각종 세균이 살고 있는 것으로 조사됐다. 외부 유해자극으로부터의 1차 방어벽으로 자신에게 도움이 되는 미생물과 유해 미생물이 서로 공존하며 피부 면역을 유지하지만 호르몬 변화, 미세먼지, 물리적 자극, 화학 성분 등으로 미생물 균형이 깨져 피부 장벽이 약화되면 각종 염증과 색소 침착, 피부노화 등 피부 문제를 일으킨다. 피부에서의 항균의 목적은 염증을 완화시켜 피부 문제를 예방하는 것이다. 여기서 문제성 피부는 여드름피부, 피지분비 조절이 어려운 피부, 스트레스로 피부 트러블이 잦은 경우를 말한다. 항균 효능 성분으로 피부의 유/수분 밸런스를 맞춰서 건강한 피부를 회복하도록 한다.In addition, according to research results published in the 'Science Journal' by a research team at the National Genome Research Institute, it was found that about 500 to 1,000 different types of bacteria live on the skin. As the first line of defense against external harmful stimuli, helpful and harmful microorganisms coexist to maintain skin immunity. However, when the microbial balance is broken due to hormonal changes, fine dust, physical stimulation, chemical components, etc., the skin barrier is weakened, causing various skin diseases. It causes skin problems such as inflammation, pigmentation, and skin aging. The purpose of antibacterial use on the skin is to relieve inflammation and prevent skin problems. Here, problematic skin refers to acne skin, skin that has difficulty controlling sebum secretion, and frequent skin problems due to stress. Antibacterial ingredients help restore healthy skin by adjusting the oil/moisture balance of the skin.
본 발명의 목적은 두켜부채, 해당화, 여뀌의 혼합 추출물을 포함하는 항산화, 항염 효과가 우수한 화장품 조성물을 제공하는 데 있다.The purpose of the present invention is to provide a cosmetic composition with excellent antioxidant and anti-inflammatory effects, containing mixed extracts of Dukwichae, Gyeonghwa, and Yeokwi.
본 발명의 다른 목적은 두켜부채, 해당화, 여뀌의 혼합 추출물을 이용하여 항균활성 및 항균효과를 나타내 피부의 산화적 스트레스와 염증반응 억제에 효능을 보이는 갖는 화장품 조성물을 제공하는 데 있다.Another object of the present invention is to provide a cosmetic composition that exhibits antibacterial activity and antibacterial effect by using mixed extracts of P. lily vulgaris, Glycyrrhiza vulgaris, and L. vulgaris, and is effective in suppressing oxidative stress and inflammatory reactions in the skin.
상기 목적을 달성하기 위한 본 발명의 화장료 조성물은 두켜부채, 해당화 및 여뀌의 알칼리 이온수 저온 침출 혼합 추출물을 유효성분으로 함유하는 것을 특징으로 한다.The cosmetic composition of the present invention for achieving the above object is characterized by containing a mixed extract of alkaline ionized water and low-temperature leaching of Dukwichae, Gunghwa and Yeokwi as active ingredients.
바람직하게는, 유효성분으로는 전체 중량에 대하여 0.01~10 중량% 함유하는 것을 특징으로 한다.Preferably, the active ingredient is contained in an amount of 0.01 to 10% by weight based on the total weight.
바람직하게는, 두켜부채, 해당화, 여뀌는 각각 1~3 : 1~3 : 1~3 의 중량 비율로 혼합하여 알칼리 이온수로 저온 침출하여 추출한 것을 특징으로 한다.Preferably, dukeobuchae, glycosuria, and yeokyeui are mixed at a weight ratio of 1-3:1-3:1-3, respectively, and extracted by low-temperature leaching with alkaline ion water.
본 발명의 두켜부채 추출물, 해당화 추출물, 여뀌 추출물을 함유하는 화장료 조성물은 항산화, 항염 효과가 우수하여 피부 염증 및 트러블을 완화시킬 수 있는 효과가 있다.The cosmetic composition containing the Prickly pear extract, the glycolytic flower extract, and the prickly pear extract of the present invention has excellent antioxidant and anti-inflammatory effects and is effective in alleviating skin inflammation and skin troubles.
또한, 본 발명의 화장료 조성물은 항균활성 및 항균효과를 나타내 피부의 산화적 스트레스와 염증반응 억제에 효능을 갖는다.In addition, the cosmetic composition of the present invention exhibits antibacterial activity and antibacterial effect and is effective in suppressing oxidative stress and inflammatory response in the skin.
또한, 본 발명의 화장료 조성물은 DPPH 소거능을 갖는다.Additionally, the cosmetic composition of the present invention has DPPH scavenging ability.
이하, 본 발명을 구체적으로 설명한다.Hereinafter, the present invention will be described in detail.
본 발명의 화장료 조성물은 두켜부채 추출물, 해당화 추출물 및 여뀌 추출물의 혼합 추출물을 유효성분으로 포함한다. 특히, 혼합 추출물은 전체 중량에 대하여 0.01~10 중량% 함유하는 것이 바람직하다. 이때, 0.01 중량% 미만이면 본 발명의 목적에 부합하지 않고, 10 중량%를 초과하면 화장료 조성물 내에서 불안정하여 석출될 가능성이 있다.The cosmetic composition of the present invention contains as active ingredients a mixed extract of P. chinensis extract, Glycorrhizae extract, and D. lily extract. In particular, the mixed extract is preferably contained in an amount of 0.01 to 10% by weight based on the total weight. At this time, if it is less than 0.01% by weight, it does not meet the purpose of the present invention, and if it exceeds 10% by weight, it is unstable and may precipitate within the cosmetic composition.
본 발명의 두켜부채(Distromium decumbens)는 바다에 나는 갈조류로 식물체는 암반에 착생한다. 국내에서는 1종이 제주도, 울릉도, 남해안에서 보고되었다. 또한, 우리나라 동·남해안의 조간대 및 조하대에 서식하며, 일본, 호주 남 부 지역에 분포하는 것으로 알려져 있다. 몸의 지름은 2 내지 4cm, 몸의 하부는 오래되면 두꺼워지고 때로는 줄기모양을 형성한다. 빛깔은 엷은 갈색 또는 짙은 갈색이며, 색소체를 지닌 두 세포층으로 구성되어 있다.Distromium decumbens of the present invention is a brown algae that grows in the sea and the plant grows on rock. In Korea, one species was reported from Jeju Island, Ulleungdo, and the southern coast. In addition, it is known to live in the intertidal and subtidal zones of the eastern and southern coasts of Korea, and to be distributed in Japan and southern Australia. The body diameter is 2 to 4 cm, and the lower part of the body becomes thick as it ages and sometimes forms a stem shape. It is light or dark brown in color and consists of two cell layers containing plastids.
해당화(Rosa rugosaThunb.)는 장미과에 속하는 낙엽관목으로 가시에 털이 있으며 바다가 모래 땅에 잘 자란다, 꽃은 민괴화라 하여 중국 및 일본에서는 토혈, 하리, 월경과다 등에 이용되고 있다. 이식물의 잎, 꽃, 과실, 지하부등에서 화학 성분 연구가 이루어져 있으며, 일본에서는 꽃의 색소를 천연 착색료로 이용하기도 하고 특히, 우리나라에서는 해당화 지하부를 당뇨병 치료제로 사용하고 있다.Rosa rugosaThunb. is a deciduous shrub belonging to the Rosaceae family. It has hairs on thorns and grows well in sandy soil. The flower is called Mingoihwa and is used in China and Japan for coughing up blood, harem, and menorrhagia. Chemical composition studies are being conducted on the leaves, flowers, fruits, and underground parts of transplants. In Japan, the pigments of flowers are used as natural colorants, and in particular, in Korea, the underground parts of the flower are used as a treatment for diabetes.
여뀌(Persicaria hydropiper L.)는 북반구 온대 및 난대지방에 걸쳐 분포하는 마디풀목 마디풀과의 1년생 초본으로 흔히 습지 또는 물가에서 야생한다. 줄기는 40~100cm 정도로 자라고 가지가 많이 갈라지며 털이 없고, 어긋나게 달리는 잎은 잎자루가 없는 피침형으로 양끝이 좁고 표면에 털이 거의 없다. 열매는 꽃받침에 싸여 검게 익는다. 꽃, 잎, 열매는 식용으로 사용할 수 있는데, 씹으면 매운맛이 난다. 지혈작용이 있어 자궁출혈, 치질출혈 및 그 밖의 내출혈에 사용하며, 잎과 줄기는 항균작용이 뛰어나고, 혈압을 내려주며 소장과 자궁의 긴장도를 강화시킨다.Persicaria hydropiper L. is an annual herb of the Nodule family, distributed throughout the temperate and subtropical regions of the Northern Hemisphere, and often grows wild in wetlands or near water. The stem grows to about 40~100cm, has many branches and is hairless, and the leaves that grow alternately are lanceolate without petioles, narrow at both ends, and have almost no hairs on the surface. The fruit is wrapped in a calyx and ripens black. The flowers, leaves, and fruits can be used as food, and have a spicy taste when chewed. It has a hemostatic effect and is used for uterine bleeding, hemorrhoidal bleeding and other internal bleeding. The leaves and stems have excellent antibacterial properties, lower blood pressure and strengthen the tension of the small intestine and uterus.
이러한, 두켜부채, 해당화 및 여뀌는 각각 1~3 : 1~3 : 1~3의 중량 비율로 혼합된 후에 알칼리 이온수로 저온 침출되는 것이 바람직하다. 알칼리 이온수를 사용하면 항산화 물질의 활성을 유지하고 미생물의 번식을 감소시키는 장점이 있다.It is preferable that these two-layer fan, sugar-coated flower, and yellowtail are mixed at a weight ratio of 1-3:1-3:1-3, respectively, and then leached at low temperature with alkaline ion water. Using alkaline ionized water has the advantage of maintaining the activity of antioxidants and reducing the proliferation of microorganisms.
또한, 본 발명의 화장료 조성물은 그 제형에 있어서 특별히 한정되는 바가 없으며, 예를 들면, 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 밀크로션, 모이스처로션, 영양로션, 맛사지크림, 영양크림, 모이스처 크림, 핸드크림, 에센스, 팩 등의 제형을 가질 수 있다. 또한 비누, 샴푸, 클렌징폼, 클렌징로션, 클렌징크림 ,바디로션, 바디클렌저 등의 제형일 수도 있다.In addition, the cosmetic composition of the present invention is not particularly limited in its formulation, and includes, for example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, It can have formulations such as moisture cream, hand cream, essence, and pack. It may also be in the form of soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, etc.
또한, 각 제형의 화장료 조성물에 있어서, 상기의 두켜부채, 해당화 및 여뀌의 혼합 추출물 외에 다른 성분들을 기타 화장료의 제형 또는 사용목적 등에 따라 임의로 선정하여 배합할 수 있다. 예를 들어, 용제, 보습제, 방부제, 살균제, 유화제, 유기 및 무기 안료, 자외선 흡수제, 착색제, 착향제, pH조절제 등을 포함할 수 있다.In addition, in the cosmetic composition of each formulation, other ingredients in addition to the mixed extracts of the above-mentioned Dukwichae, Gyeonghwa, and Yeokwi can be arbitrarily selected and mixed depending on the formulation or purpose of use of the other cosmetics. For example, it may include solvents, moisturizers, preservatives, disinfectants, emulsifiers, organic and inorganic pigments, ultraviolet absorbers, colorants, flavoring agents, pH adjusters, etc.
이하, 본 발명을 실험예를 바탕으로 구체적으로 설명하면 다음과 같다. 이러한 발명의 실험예는 본 발명을 예시하기 위한 것이며, 본 발명의 범주가 이들만으로 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail based on experimental examples. These experimental examples of the present invention are intended to illustrate the present invention, and the scope of the present invention is not limited to these only.
<실시예 1 ~ 10> : 두켜부채, 해당화 및 여뀌의 혼합 추출물의 제조<Examples 1 to 10>: Preparation of mixed extracts of Dukyeopchae, Gyeonghwa and Yeoquia
1) 혼합 추출물 생성 단계1) Mixed extract generation step
건조된 두켜부채, 해당화 및 여뀌 혼합물의 혼합 추출물을 아래 [표 1]의 배합비로 혼합하였다. 추출용매로서 알칼리 이온수 10배 중량을 넣고 3~5℃, 24시간 침출하였다. 위의 방법으로 추출한 뒤 3일간 실온에서 방치하여 침전물을 300메쉬 여과지로 여과하고, 침전물을 에드벤텍 5번 여과지와 와트만 GFC 150mm 여과지로 2번 여과하였다. 그리고 감압 농축기(Coolace CCA-1100, EYELA)를 이용하여 40℃~50℃의 온도에서 농축한 후 스프레이 드라이어(B-290, BUCHI사)를 이용하여 인렛(Inlet) 온도 180℃, 흡입기(Aspirator) 효율 100%(35㎤/시간), 펌프(Pump) 효율 25%(7.5㎖/분), 노즐클리너 4(Nozzle Cleaner 4) 및 유량계(Rotameter): 30㎜(357리터/시간)의 조건으로 건조시켜 추출분말을 얻었다. The mixed extracts of the dried snail, perilla, and snail mixture were mixed in the mixing ratio shown in [Table 1] below. As an extraction solvent, 10 times the weight of alkaline ionized water was added and leached at 3-5°C for 24 hours. After extraction using the above method, it was left at room temperature for 3 days and the precipitate was filtered through a 300 mesh filter paper, and the precipitate was filtered twice through an Edventec No. 5 filter paper and a Whatman GFC 150 mm filter paper. After concentrating at a temperature of 40℃~50℃ using a vacuum concentrator (Coolace CCA-1100, EYELA), the inlet temperature is 180℃ using a spray dryer (B-290, BUCHI) and an aspirator. Dry under the conditions of 100% efficiency (35㎤/hour), pump efficiency 25% (7.5㎖/min), Nozzle Cleaner 4 and Rotameter: 30㎜ (357 liters/hour) Extract powder was obtained.
2) 멸균 단계2) Sterilization step
알칼리 이온수로 저온 침출된 두켜부채, 해당화 및 여뀌의 혼합 추출물을 멸균한다. 멸균 방법으로는 크게 열, 자외선, 방사선 또는 고주파 등을 이용하는 물리적 방법과 약액 또는 가스를 이용하는 화학적 방법이 있으며, 유효성분을 최대한으로 손상시키지 않으면서 멸균하는 방법이 선택될 수 있다.Sterilize the low-temperature leached mixed extracts of Dukwichae, Gyeonghwa, and Eysteria chinensis with alkaline ionized water. Sterilization methods include physical methods using heat, ultraviolet rays, radiation, or high frequency, and chemical methods using chemicals or gases. A method of sterilizing the active ingredient without damaging it as much as possible can be selected.
3) 발효 단계3) Fermentation step
상기 멸균 단계를 거친 알칼리 이온수 저온 침출 두켜부채, 해당화 및 여뀌의 혼합 추출물에 발효 균주백국균을 접종하고 20 ~ 25℃에서 3-5일 발효시킨다. The mixed extract of low-temperature leached alkaline ionized water, which has gone through the sterilization step above, is inoculated with the fermented strain Baek chrysanthemum and fermented at 20 to 25°C for 3-5 days.
4) 여과 및 건조 단계4) Filtration and drying step
위의 방법으로 발효한 뒤 멸균과정을 거친 후 3일간 실온에서 방치한 후에 침전물을 300 mesh 여과지로 여과하고, 침전물을 에드벤텍 5번 여과지와 와트만 GFC 150mm 여과지로 2번 여과하였다. 그리고 감압 농축기(Coolace CCA-1100, EYELA)를 이용하여 40℃~50℃의 온도에서 농축한 후 스프레이 드라이어(B-290, BUCHI사)를 이용하여 인렛(Inlet) 온도 180℃, 흡입기(Aspirator) 효율 100%(35㎤/시간), 펌프(Pump) 효율 25%(7.5㎖/분), 노즐클리너 4(Nozzle Cleaner 4) 및 유량계(Rotameter) : 30㎜(357리터/시간)의 조건으로 건조시켜 표제의 추출분말을 얻었다.After fermentation using the above method, sterilization process, and leaving at room temperature for 3 days, the precipitate was filtered through a 300 mesh filter paper, and the precipitate was filtered twice through an Edventec No. 5 filter paper and a Whatman GFC 150 mm filter paper. After concentrating at a temperature of 40℃~50℃ using a vacuum concentrator (Coolace CCA-1100, EYELA), the inlet temperature is 180℃ using a spray dryer (B-290, BUCHI) and an aspirator. Dry under the conditions of 100% efficiency (35㎤/hour), pump efficiency 25% (7.5㎖/min), Nozzle Cleaner 4 and Rotameter: 30㎜ (357 liters/hour) This was performed to obtain the title extract powder.
<실험예 1 : 세포 독성 여부 확인><Experimental Example 1: Confirmation of cytotoxicity>
MTT assay법은 MTT[3-(4,5-dimethythiasol-2-yl)-2,5-diphenyl tetrazolium bromide] 시약이 세포 내로 흡수된 후 미토콘드리아의 숙신산탈수소효소(succinate dehydrogenase)에 의해 포마잔(formazan)을 형성하는데 이 물질의 세포 내 축적은 미토콘드리아의 활성, 넓게는 세포의 활성을 의미하는 것으로써 세포의 생존율을 측정하는 대표적인 방법이다.In the MTT assay, the MTT [3-(4,5-dimethythiasol-2-yl)-2,5-diphenyl tetrazolium bromide] reagent is absorbed into the cell and then formazan is activated by mitochondrial succinate dehydrogenase. ), and the accumulation of this substance within the cell indicates mitochondrial activity, broadly speaking, cell activity, and is a representative method of measuring cell survival.
각각의 유전자 발현을 확인하기 위해 세포를 1×105cell/well의 밀도로 96 well에 200㎕ 배지와 함께 분주한 뒤 5% CO2, 37℃ incubator에서 24시간 배양한 후 배지를 버리고 PBS로 씻어준 다음 같은 양의 배지와 PBS에 녹인 실시예 1 ~ 10의 혼합 추출물 시료를 0.1%에서 5.0%까지 다양한 농도에 따라 각 well에 처리하여 24시간 배양하였다. 배양이 끝나기 4시간 전에 PBS에 녹인 5㎎/㎖ MTT를 20㎕씩 각 well에 첨가하고 알루미늄 호일로 차광시킨 후 3간 동안 5% CO2및 37℃ 조건의 incubator에서 배양하였다. 배양액을 제거하고 DMSO용액 200㎕를 첨가하여 37℃ incubator에서 1시간 반응 시킨 후 ELISA reader를 이용하여 570nm에서 흡광도를 측정하여 비교하였다. 세포 생존율은 하기 식에 의해 산출되었고, 그 결과는 하기 [표 2]에 나타내었다.To check the expression of each gene , cells were dispensed at a density of 1 After washing, the mixed extract samples of Examples 1 to 10 dissolved in the same amount of medium and PBS were added to each well at various concentrations from 0.1% to 5.0% and cultured for 24 hours. 4 hours before the end of the culture, 20㎕ of 5㎎/㎖ MTT dissolved in PBS was added to each well, blocked from light with aluminum foil, and cultured in an incubator at 5% CO 2 and 37°C for 3 hours. The culture medium was removed, 200 ㎕ of DMSO solution was added, and the mixture was incubated for 1 hour in an incubator at 37°C. Then, the absorbance was measured and compared at 570 nm using an ELISA reader. Cell survival rate was calculated by the following formula, and the results are shown in [Table 2] below.
세포생존율(%) = 시료첨가군의 흡광도/대조군의흡광도 × 100Cell viability (%) = absorbance of sample added group/absorbance of control group × 100
상기 [표 2]의 결과에서 보는 바와 같이, 상기 실시예 1 ~ 10의 혼합 추출물들을 적용한 시료 모두 세포생존율이 확인됨에 따라 세포 독성에는 영향을 미치지 않는 것으로 확인되었고, 이에 안전에는 문제가 없는 것을 확인하였다.As can be seen from the results in [Table 2], it was confirmed that cell viability was confirmed in all samples to which the mixed extracts of Examples 1 to 10 were applied and that there was no effect on cytotoxicity, confirming that there was no problem with safety. did.
<시험예2 : 항산화 시험(DPPH free radical 소거능 측정)><Test Example 2: Antioxidation test (DPPH free radical scavenging ability measurement)>
DPPH free radical 소거능은 Nanjo et al.(1996)의 방법에 따라 측정하였다. 실시예 1 ~ 10의 시료 30㎕에 60μM DPPH(1,1-diphenyl-2-picrylhydrazyl) 용액 30㎕를 혼합 후, 반응액을 100㎕ quartz capillary tube에 옮겨 2분 후 electron spin resonance(ESR) spectrometer(JES-FA, JEOL Ltd., Tokyo, Japan)로 측정하였다. 실험조건은 다음과 같다. DPPH free radical scavenging ability was measured according to the method of Nanjo et al. (1996). After mixing 30 μl of the 60 μM DPPH (1,1-diphenyl-2-picrylhydrazyl) solution with 30 μl of the samples of Examples 1 to 10, the reaction solution was transferred to a 100 μl quartz capillary tube and measured on an electron spin resonance (ESR) spectrometer after 2 minutes. (JES-FA, JEOL Ltd., Tokyo, Japan). The experimental conditions are as follows.
magnetic field, 336.5±5 mT; power, 5 mW; modulation frequency, 9.41 GHz; amplitude, 1×1,000; sweep time, 30 s; temperature, 298 Kmagnetic field, 336.5±5 mT; power, 5 mW; modulation frequency, 9.41 GHz; amplitude, 1×1,000; sweep time, 30 s; temperature, 298 K
DPPH radical 소거능은 H 와 H0의 상대적인 radical signal peak 높이의 차에 하여 계산하였다. 그 결과를 하기 [표 3]에 나타내었다DPPH radical scavenging ability was calculated based on the difference in the relative radical signal peak heights of H and H 0 . The results are shown in [Table 3] below.
[표 3]에서 확인되는 바와 같이, 실시예 8 ~ 10의 혼합 추출물에서 DPPH free radical 소거능이 높다는 것을 알 수 있다.As confirmed in [Table 3], it can be seen that the mixed extracts of Examples 8 to 10 have high DPPH free radical scavenging ability.
<시험예 3 : 프로피오니박테리움아크네스에 대한 항균활성의 시험><Test Example 3: Test of antibacterial activity against Propionibacterium acnes>
실시예1 ~ 10의 혼합 추출물에 대하여, 평판배지확산법(Agar diffusion method)을 이용하여 프로피오니박테리움아크네스(Propionibacterium acnes)에 대한 항균활성을 측정하였다. 본 시험에 사용된 프로피오니박테리움아크네스(Propionibacterium acnes 3314)균주는 한국생명공학연구원 생물자원센터에서 분양받아 사용하였다. plate에 Brain Heart Infusion agar를 멸균하여 plate당 25㎖씩 분주하였다. 균주 도말 각각의 균주의 균수를 106으로 맞춰 1000㎕ 도말하였다. 10% Ethanol에 녹인 실시예 1~10의 혼합 추출물 시료를 0.1%, 0.5%, 1.0% 농도로 맞춘 후 40㎕씩 disc(paper disk(10mm), Advantec Filter Paper, Toyo Roshi Kaisha Ltd, Japan)에 loading 하였다. 양성 대조군으로 Salicylic acid를 사용하였으며 10% Ethanol에 녹인 Salicylic acid를 1.0% 농도로 맞춘 후 40㎕씩 disc(paper disk(10mm), Advantec Filter Paper, Toyo Roshi Kaisha Ltd, Japan)에 loading 하였다. 멸균된 핀셋으로 loading된 disc를 집어서 균이 도말된 plate에 심었다. 산소를 차단하는 anaerobic jar에 plate를 넣은 상태로 37℃에 배양한다. paper disk 주변에 형성된 원형 발육 저지환의 크기(mm 단위)를 측정하였으며, 모든 시험은 독립적으로 3회 반복하여 평균값을 구하였다. 그 결과를 하기 [표 4]에 나타내었다.For the mixed extracts of Examples 1 to 10, the antibacterial activity against Propionibacterium acnes was measured using the agar diffusion method. The Propionibacterium acnes 3314 strain used in this test was purchased from the Korea Research Institute of Bioscience and Biotechnology Biological Resources Center. Brain Heart Infusion agar was sterilized on the plate and dispensed at 25 ml per plate. Strain smear The number of bacteria of each strain was set to 10 6 and 1000㎕ was smeared. The mixed extract samples of Examples 1 to 10 dissolved in 10% Ethanol were adjusted to concentrations of 0.1%, 0.5%, and 1.0%, and then placed on a disc (paper disk (10 mm), Advantec Filter Paper, Toyo Roshi Kaisha Ltd, Japan) in an amount of 40 μl. loading was done. Salicylic acid was used as a positive control, and salicylic acid dissolved in 10% Ethanol was adjusted to a concentration of 1.0% and loaded onto a disc (paper disk (10mm), Advantec Filter Paper, Toyo Roshi Kaisha Ltd, Japan) at 40㎕. The loaded disc was picked up with sterilized tweezers and planted on the plate smeared with bacteria. Place the plate in an anaerobic jar that blocks oxygen and incubate at 37°C. The size (in mm) of the circular stunted ring formed around the paper disk was measured, and all tests were independently repeated three times and the average value was obtained. The results are shown in [Table 4] below.
상기 [표 4]에서 확인할 수 있는 바와 같이, 실시예 1 ~ 10의 혼합 추출물은 농도 의존적으로 Clean Zone의 넓이가 증가하는 것을 볼 수 있다. 특히 실시예 8~10의 혼합 추출물은 다른 실시예의 추출물들에 비하여 우수한 항균활성을 나타내는 것을 확인할 수 있었다. 특히 실시예 10은 프로피오니박테리움아크네스(Propionibacterium acnes)에 대한 항균활성 효과가 매우 우수한 것을 확인할 수 있었다.As can be seen in [Table 4], the area of the clean zone of the mixed extracts of Examples 1 to 10 increases in a concentration-dependent manner. In particular, it was confirmed that the mixed extracts of Examples 8 to 10 exhibited excellent antibacterial activity compared to the extracts of other examples. In particular, Example 10 was confirmed to have excellent antibacterial activity against Propionibacterium acnes.
<시험예 4 : 염증매개인자 발현 억제효과 확인><Test Example 4: Confirmation of the effect of suppressing the expression of inflammatory mediators>
RAW264.7 세포를 10 % FBS가 첨가된 DMEM 배지를 이용하여 5×105cell/well로 조절한 후, 12 well plate에 접종하고 18시간 동안 배양하였다. 배양한 뒤 12시간 동안 기아상태를 유지시킨 후, LPS 10 ng/mL와 실시예 1~12의 진공저온 발효추출물 시료를 처리하고 24시간 동안 배양하였다. 배양된 세포에서 RNA를 추출하여 Reverse transcription polymerase chain reaction(RT-PCR)을 통해 TNF-α의 발현 억제율을 확인하였다. TNF-α 발현억제율은 하기식에 의해 산출되었고, 그 결과는 하기 [표 5]에 나타내었다.RAW264.7 cells were adjusted to 5 × 10 5 cells/well using DMEM medium supplemented with 10% FBS, then inoculated into a 12 well plate and cultured for 18 hours. After culturing and maintaining starvation for 12 hours, 10 ng/mL of LPS and the vacuum low-temperature fermentation extract samples of Examples 1 to 12 were treated and cultured for 24 hours. RNA was extracted from cultured cells and the inhibition rate of TNF-α expression was confirmed through reverse transcription polymerase chain reaction (RT-PCR). The inhibition rate of TNF-α expression was calculated by the following formula, and the results are shown in [Table 5] below.
TNF-α 발현억제율(%) = [(대조군 TNF-α발현양 - 시료 TNF-α발현양)/ 대조군TNF-α 발현양] × 100TNF-α expression inhibition rate (%) = [(control group TNF-α expression level - sample TNF-α expression level) / control group TNF-α expression level] × 100
상기 [표 5]에서 확인되는 바와 같이 실시예 8~10의 혼합 추출물에서 보다 우수한 염증매개인자 발현 억제효과를 나타냄을 확인할 수 있었다.As confirmed in [Table 5] above, it was confirmed that the mixed extracts of Examples 8 to 10 exhibited a better effect of suppressing the expression of inflammatory mediators.
Claims (5)
A cosmetic composition containing mixed extracts of Deukwichae, Gyeonghwa and Yeoqui as active ingredients.
The cosmetic composition according to claim 1, wherein the dukeobuchae, glycolic acid, and yeokwi are mixed at a weight ratio of 1-3:1-3:1-3, respectively, and leached at low temperature with alkaline ion water.
The cosmetic composition according to claim 1, wherein the mixed extract is contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition.
건조된 두켜부채, 해당화 및 여뀌를 혼합하고 알칼리 이온수에서 침지시키고 침전물을 여과하고 감압 농축시킨 후 건조시켜 추출분말을 생성하는 단계;
추출분말을 멸균하는 단계;
멸균된 추출분말에 발효 균주를 접종하여 발효시키는 단계;
발효한 뒤 멸균 과정을 거친 후 침전물을 여과하고, 농축한 후 건조시켜 혼합 추출물 분말을 생성하는 단계;
로 생성되는 것을 특징으로 하는 화장료 조성물.
The method of claim 1, wherein the mixed extract is
A step of mixing dried dukeupan, glycolic acid, and yeokyo, immersing them in alkaline ionized water, filtering the precipitate, concentrating under reduced pressure, and drying to produce an extract powder;
Sterilizing the extraction powder;
Inoculating the sterilized extract powder with a fermentation strain and fermenting it;
After fermentation, going through a sterilization process, filtering the sediment, concentrating it, and drying it to produce a mixed extract powder;
A cosmetic composition characterized in that it is produced.
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| KR102059392B1 (en) | 2018-05-23 | 2019-12-26 | (주)에스디생명공학 | Antimicrobial and Antifungal Composition Containing Clove, Hibiscus, and Coconut Extract Complex as Active Ingredient |
| KR20210141085A (en) | 2020-05-15 | 2021-11-23 | 주식회사 가나씨앤피 | Antimicrobial and Antifungal Cosmetic Composition Containing Complex Extract of Chamaecyparis obtusa, Rosa rugosa, and Melissa officinalis as Active Ingredient |
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