KR20230137014A - Antimicrobial composition comprising preservative compound and reuterin and use thereof - Google Patents
Antimicrobial composition comprising preservative compound and reuterin and use thereof Download PDFInfo
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- KR20230137014A KR20230137014A KR1020220034592A KR20220034592A KR20230137014A KR 20230137014 A KR20230137014 A KR 20230137014A KR 1020220034592 A KR1020220034592 A KR 1020220034592A KR 20220034592 A KR20220034592 A KR 20220034592A KR 20230137014 A KR20230137014 A KR 20230137014A
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- KR
- South Korea
- Prior art keywords
- weight
- reuterin
- preservative
- cosmetic
- antibacterial
- Prior art date
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- 239000003755 preservative agent Substances 0.000 title claims abstract description 92
- 239000000203 mixture Substances 0.000 title claims abstract description 78
- 230000002335 preservative effect Effects 0.000 title claims abstract description 69
- AKXKFZDCRYJKTF-UHFFFAOYSA-N 3-Hydroxypropionaldehyde Chemical compound OCCC=O AKXKFZDCRYJKTF-UHFFFAOYSA-N 0.000 title claims abstract description 57
- 150000001875 compounds Chemical class 0.000 title claims abstract description 53
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- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 70
- 241000894006 Bacteria Species 0.000 claims description 23
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 claims description 22
- 229940100524 ethylhexylglycerin Drugs 0.000 claims description 16
- NCZPCONIKBICGS-UHFFFAOYSA-N 3-(2-ethylhexoxy)propane-1,2-diol Chemical compound CCCCC(CC)COCC(O)CO NCZPCONIKBICGS-UHFFFAOYSA-N 0.000 claims description 15
- 239000004299 sodium benzoate Substances 0.000 claims description 15
- 235000010234 sodium benzoate Nutrition 0.000 claims description 15
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- GHBFNMLVSPCDGN-UHFFFAOYSA-N rac-1-monooctanoylglycerol Chemical compound CCCCCCCC(=O)OCC(O)CO GHBFNMLVSPCDGN-UHFFFAOYSA-N 0.000 claims description 13
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
본 발명은 미생물의 활성을 저해하거나 미생물 사멸을 위한 방부 화합물 및 루테린을 포함하는 항균용 조성물, 화장료 조성물 및 화장품 보존용 방부제에 관한 것으로, 상기 항균용 조성물은 방부 화합물 및 루테린을 포함함으로써 방부 화합물의 사용량을 최소화하여 제형 안정성을 개선시킬 수 있고 루테린 단독 사용에 비해 사용량이 감소되어 제형 내 항균력을 확보할 수 있어 방부 화합물 또는 루테린의 단점을 보완한 화장료 조성물, 화장품 보존용 방부제 등으로 활용될 수 있다.The present invention relates to an antibacterial composition containing a preservative compound and reuterin for inhibiting the activity of microorganisms or killing microorganisms, a cosmetic composition, and a preservative for preserving cosmetics. The antibacterial composition contains a preservative compound and reuterin, thereby preservative The formulation stability can be improved by minimizing the amount of compound used, and the antibacterial effect in the formulation can be secured by reducing the amount used compared to the use of reuterin alone, so it can be used as a preservative compound or a cosmetic composition that complements the shortcomings of reuterin, a preservative for cosmetics preservation, etc. It can be utilized.
Description
본 발명은 미생물의 활성을 저해하거나 미생물 사멸을 위한 방부 화합물 및 루테린을 포함하는 항균용 조성물, 화장료 조성물 및 화장품 보존용 방부제에 관한 것이다.The present invention relates to an antibacterial composition containing reuterin and a preservative compound for inhibiting the activity of microorganisms or killing microorganisms, a cosmetic composition, and a preservative for preserving cosmetics.
미생물은 유기물 또는 유기 화합물을 분해하여 사람에게 유용한 물질을 생성하기도 하지만 악취와 함께 사람에게 해로운 물질을 생성하며 화학적, 생물학적으로 변질시킨다. 특히 화장품의 경우, 제조공정 중 미생물에 의한 오염, 사용 중 손가락 등의 피부 접촉에 의한 미생물 오염 등을 피할 수 없기 때문에 통상 미생물의 증식을 억제하고 보존 과정에서 미생물을 사멸시켜 제품의 보존성을 향상시키기 위해서는 방부제의 사용이 불가피하다. Microorganisms decompose organic matter or organic compounds to produce substances useful to humans, but they also produce substances harmful to humans along with bad odors and deteriorate them chemically and biologically. In particular, in the case of cosmetics, contamination by microorganisms during the manufacturing process and contamination by skin contact with fingers or other skin during use cannot be avoided, so it is usually necessary to suppress the growth of microorganisms and kill microorganisms during the preservation process to improve the preservability of the product. For this purpose, the use of preservatives is inevitable.
방부제는 화장품에 배합되어 외부로부터 화장품을 오염시키는 미생물의 증식을 억제하며, 시간 경과와 함께 미생물을 사멸시켜 제품의 변화를 방지한다. 주로 사용되는 방부제로는 화학 물질을 포함하는 합성 방부제로, 파라옥시안식향산에스테르 (파라벤류), 이미다졸리디닐우레아, 페녹시에탄올 등이 있으며, 2개의 수산기(hydroxyl group)를 가지는 디올(diol)계 화합물이나 계면활성제도 방부제로 사용될 수 있다. 그러나 합성 방부제 중 파라벤류는 알러지 반응 및 환경호르몬으로서의 가능성, 내성균 유발 등의 문제점이 있으며, 디올계 화합물이나 계면활성제는 항진균 활성이 약하고 제품을 묽게 만들어 제형 안정성에 문제가 있고 피부 자극이 크다는 점도 지적되고 있다. 이러한 합성 방부제의 안정성 및 유해성이 알려지면서 소비자의 불안이 가중되고 있다. 합성 방부제의 사용량을 줄이려는 노력이 있으나, 방부제가 첨가되지 않거나 적절한 양에 못 미치는 수준으로 첨가되면 제품의 보관기간이 짧아지고 제품 변질 및 미생물 오염이 문제가 되기 때문에 방부제의 유해성을 인지하고도 방부제를 사용할 수 밖에 없는 현실이다.Preservatives are mixed into cosmetics to suppress the growth of microorganisms that contaminate cosmetics from the outside, and prevent changes in the product by killing microorganisms over time. Mainly used preservatives are synthetic preservatives containing chemical substances, such as paraoxybenzoic acid ester (parabens), imidazolidinyl urea, phenoxyethanol, etc., and diol with two hydroxyl groups. Based compounds or surfactants can also be used as preservatives. However, among synthetic preservatives, parabens have problems such as allergic reactions, the possibility of acting as environmental hormones, and causing resistant bacteria. It is also pointed out that diol-based compounds and surfactants have weak antifungal activity and dilute the product, causing problems with formulation stability and causing significant skin irritation. It is becoming. As the safety and harmfulness of these synthetic preservatives become known, consumer anxiety is increasing. There are efforts to reduce the amount of synthetic preservatives used, but if preservatives are not added or are added in less than appropriate amounts, the storage period of the product is shortened and product deterioration and microbial contamination become problems, so even though the harmfulness of preservatives is recognized, preservatives are used. The reality is that we have no choice but to use .
이러한 합성 방부제의 문제점을 극복하기 위해 자연에서 얻은 소재를 이용하거나 이를 포함하는 천연 방부제를 개발하는 연구가 활발히 진행되고 있다. 그 예로, 아니스, 마치현, 병풀, 은행잎, 오매, 쇠뜨기잎 등의 식물에서 추출한 추출물을 합성 방부제와 혼합하여 사용한 시도도 있었다. 그러나 기존 합성 방부제 대비 사용량을 줄이면서 제품의 성능 및 안정성을 모두 확보할 수 있는 방부제에 대한 연구 및 개발이 여전히 요구되고 있다.To overcome these problems with synthetic preservatives, research is being actively conducted to develop natural preservatives using or containing materials obtained from nature. For example, there was an attempt to use extracts from plants such as anise, portulaca, centella asiatica, ginkgo biloba, quince, and horsetail leaf mixed with synthetic preservatives. However, research and development on preservatives that can ensure both product performance and stability while reducing the amount used compared to existing synthetic preservatives are still required.
한편, 천연 항균 물질인 루테린은 넓은 스펙트럼의 항균력을 보이는 물질로 보고되고 있다. 그러나, 아미노산이 존재하는 조건에서 항균력이 떨어지므로 아미노산이 포함된 제품에 적용하는데 어려움이 있다 (염은미 외, 한국식품과학회지 2004. 36(1): 111-115).Meanwhile, reuterin, a natural antibacterial substance, is reported to have a broad spectrum of antibacterial properties. However, because the antibacterial activity is low in the presence of amino acids, it is difficult to apply it to products containing amino acids (Eunmi Yeom et al., Journal of the Korean Society of Food Science, 2004. 36(1): 111-115).
따라서 합성 방부제, 루테린 등 기존 방부제의 단점을 극복하기 위해서는 많은 연구가 필요한 실정이다.Therefore, much research is needed to overcome the shortcomings of existing preservatives such as synthetic preservatives and lutein.
본 발명은 세균, 진균 등 미생물의 활성을 저해하거나 미생물 사멸을 위한 항균용 조성물, 화장료 조성물 및 화장품 보존용 방부제를 제공하는 것을 목적으로 한다.The purpose of the present invention is to provide an antibacterial composition, a cosmetic composition, and a preservative for preserving cosmetics that inhibit the activity of microorganisms such as bacteria and fungi or kill microorganisms.
본 발명의 일 양상은 방부 화합물 및 루테린을 유효성분으로 포함하는 항균용 조성물을 제공한다.One aspect of the present invention provides an antibacterial composition containing a preservative compound and reuterin as an active ingredient.
본 발명에서 사용된 “방부 화합물”은 물질의 화학적 또는 생물학적 변질을 막기 위해 첨가되는 화합물을 의미하며, 보존제 또는 살균제로 사용되는 화합물을 포함하기도 한다. 방부 화합물의 화학 독성을 우려하여 화장품, 식품 및 의약품에는 인체에 독성이 없는 안전한 물질이 사용되어야 하므로, 본 발명에서는 화학적으로 안전하고 무해성이 검증된 방부 화합물이라면 제한 없이 이용 가능하다.As used in the present invention, “preservative compound” refers to a compound added to prevent chemical or biological deterioration of a material, and also includes compounds used as preservatives or disinfectants. Because of concerns about the chemical toxicity of preservative compounds, safe materials that are not toxic to the human body must be used in cosmetics, food, and medicine. Therefore, in the present invention, any preservative compound that has been proven to be chemically safe and harmless can be used without limitation.
상기 방부 화합물의 일례로는 파라벤(paraben)류, 파라핀(paraffin)류, 페녹시에탄올(phenoxyethanol), 이미다졸리디닐우레아(imidazolidinyl urea), 디아졸리디닐우레아(diazolidinyl urea), 이소프로필알코올(isopropyl alcohol), 클로로펜(chlorophene), 포믹애씨드(formic acid), 피록톤올아민(piroctone olamine), 헥세티딘(hexetidine), 헥사미딘(hexamidine), 이소티아졸리논(isothiazolinone), 하이드록시아세토페논(hydroxyacetophenone), 글리세릴카프릴레이트(glyceryl caprylate), 에틸헥실글리세린(ethylhexylglycerin), 소듐벤조에이트(sodium benzoate) 등이 있으나, 이에 한정되는 것은 아니다.Examples of the preservative compounds include parabens, paraffins, phenoxyethanol, imidazolidinyl urea, diazolidinyl urea, and isopropyl alcohol. alcohol), chlorophene, formic acid, piroctone olamine, hexetidine, hexamidine, isothiazolinone, hydroxyacetophenone ( Examples include, but are not limited to, hydroxyacetophenone, glyceryl caprylate, ethylhexylglycerin, and sodium benzoate.
본 발명의 일 구체예에 따르면, 상기 방부 화합물은 하이드록시아세토페논, 글리세릴카프릴레이트, 에틸헥실글리세린 및 소듐벤조에이트로 이루어진 군에서 선택된 1종 이상인 것일 수 있다.According to one embodiment of the present invention, the preservative compound may be one or more selected from the group consisting of hydroxyacetophenone, glyceryl caprylate, ethylhexylglycerin, and sodium benzoate.
상기 하이드록시아세토페논은 식물 유래 성분으로 화장품의 천연 산화방지제로 사용되며, 파라벤류에 비해 방부력은 약하지만 피부에 더 친화적이며 파라벤을 비롯한 다른 방부제와 함께 사용할 경우 방부 기능이 증대되는 물질이다. 이러한 하이드록시아세토페논은 하이드록시기(-OH)의 위치에 따라 2-, 3- 및 4-하이드록시아세토페논으로 구분되나, 방부 기능에 있어서는 차이가 없다.The hydroxyacetophenone is a plant-derived ingredient and is used as a natural antioxidant in cosmetics. Although it has weaker preservative power than parabens, it is more friendly to the skin and has an increased preservative function when used with other preservatives, including parabens. These hydroxyacetophenones are classified into 2-, 3-, and 4-hydroxyacetophenones depending on the position of the hydroxy group (-OH), but there is no difference in preservative function.
상기 글리세릴카프릴레이트는 식물 유래 성분으로 일종의 계면활성제 역할을 하여 주로 화장품에서 유화제로 사용되며, 항세균 및 항진균 효과가 우수하여 방부대체원료로 사용 가능하다.The glyceryl caprylate is a plant-derived ingredient that acts as a kind of surfactant and is mainly used as an emulsifier in cosmetics. It has excellent antibacterial and antifungal effects and can be used as an alternative preservative material.
상기 에틸헥실글리세린은 습윤 특성이 있어 화장품의 보습 효과를 위해 주로 사용되지만 통상의 방부제인 페녹시에탄올, 이소티아졸리논, 파라벤 등의 항균력을 증가시키는 역할도 하여 이러한 방부제의 사용량을 줄이는데 사용된다. 또한, 인체에서 냄새를 유발하는 혐기성 박테리아의 성장을 억제하는 항균 활성도 있다.The ethylhexylglycerin has moisturizing properties and is mainly used for the moisturizing effect of cosmetics. However, it also increases the antibacterial activity of common preservatives such as phenoxyethanol, isothiazolinone, and paraben, and is used to reduce the amount of these preservatives used. It also has antibacterial activity that inhibits the growth of anaerobic bacteria that cause odor in the human body.
상기 소듐벤조에이트는 세균, 효모, 곰팡이 등 다양한 미생물의 생육을 억제하는 효과가 있는 화합물로, 값이 저렴하고 항균 효과가 우수할 뿐만 아니라 독성도 낮아 주로 식품 보존제로 사용되며, 화장품에서는 파라벤을 대체하는 합성 보존제 중 하나이다.The sodium benzoate is a compound that is effective in inhibiting the growth of various microorganisms such as bacteria, yeast, and mold. It is inexpensive, has excellent antibacterial effect, and has low toxicity, so it is mainly used as a food preservative and replaces paraben in cosmetics. It is one of the synthetic preservatives used.
루테린(Reuterin)은 락토바실러스 루테리(Lactobacillus reuteri)가 글리세롤로부터 생산하는 천연 항미생물 물질인 3-하이드록시프로피온알데하이드(3-hydroxypropionaldehyde)이다. 루테린은 병원성 세균뿐만 아니라 효모, 곰팡이 등에 대한 항균력을 보유하고 있어 항균 스펙트럼이 매우 넓은 특성을 가지고 있다 (Doleyres et al., Appl Microbiol Biotechnol 2005, 68: 467-474). Reuterin is 3-hydroxypropionaldehyde, a natural antimicrobial substance produced by Lactobacillus reuteri from glycerol. Reuterin has antibacterial activity against not only pathogenic bacteria, but also yeast and mold, and has a very wide antibacterial spectrum (Doleyres et al., Appl Microbiol Biotechnol 2005, 68: 467-474).
본 발명의 일 구체예에 따르면, 상기 루테린은 락토바실러스 루테리 또는 이의 배양액으로부터 분리된 것일 수 있다.According to one embodiment of the present invention, the reuterin may be isolated from Lactobacillus reuteri or its culture medium.
본 발명에서 사용된 "배양액"은 락토바실러스 루테리의 생존, 발육 또는 증식에 필요한 영양물질을 의미하며, 균주가 생산하여 외부로 분비하는 효소, 대사산물 등도 포함할 수 있다.“Culture medium” used in the present invention refers to nutrients necessary for the survival, development or proliferation of Lactobacillus reuteri, and may also include enzymes and metabolites produced by the strain and secreted to the outside.
이러한 방부 화합물 및 루테린을 포함하는 조성물은 항균 활성을 나타낼 수 있다.Compositions containing these preservative compounds and reuterin may exhibit antibacterial activity.
본 발명에서 사용된 "항균(antimicrobial)"은 어떤 농도에서 세균, 진균 등의 미생물 성장 또는 생존을 감소, 방지, 억제 또는 제거하는 능력을 의미하며, "항균용 조성물"은 이러한 항균력을 나타내는 물질을 포함하는 조성물을 의미한다.As used in the present invention, “antimicrobial” refers to the ability to reduce, prevent, suppress, or eliminate the growth or survival of microorganisms such as bacteria and fungi at a certain concentration, and “antimicrobial composition” refers to a substance that exhibits such antibacterial activity. It means a composition containing.
본 발명의 일 구체예에 따르면, 상기 항균용 조성물은 세균 및 진균에 대해 항균 활성을 가지는 것일 수 있다.According to one embodiment of the present invention, the antibacterial composition may have antibacterial activity against bacteria and fungi.
본 발명의 일 구체예에 따르면, 상기 항균용 조성물은 에세리키아(Escherichia)속 균주, 스테필로코커스(Staphylococcus)속 균주, 슈도모나스(Pseudomonas)속 균주, 칸디다(Candida)속 균주 및 아스퍼질러스(Aspergillus)속 균주로 이루어진 군에서 선택된 1종 이상의 균주에 대해 항균 활성을 가지는 것일 수 있다.According to one embodiment of the present invention, the antibacterial composition includes Escherichia genus strains, Staphylococcus genus strains, Pseudomonas genus strains, Candida genus strains, and Aspergillus ( It may have antibacterial activity against one or more strains selected from the group consisting of Aspergillus genus strains.
상기 항균용 조성물은 방부 화합물 및 루테린을 포함함으로써 기존에 항균력을 내기 위해 사용되는 방부 화합물 또는 루테린의 사용량에 비해 적은 양으로도 충분한 항균 활성을 나타내며 제품의 제형 안정성을 유지하고 보관기간을 연장할 수 있다.The antibacterial composition contains a preservative compound and reuterin, so it exhibits sufficient antibacterial activity even in a small amount compared to the amount of preservative compound or reuterin used for existing antibacterial properties, maintains the formulation stability of the product, and extends the storage period. can do.
본 발명의 일 구체예에 따르면, 상기 항균용 조성물은 전체 중량을 기준으로 방부 화합물 0.01 내지 50 중량%를 포함하는 것일 수 있다.According to one embodiment of the present invention, the antibacterial composition may contain 0.01 to 50% by weight of a preservative compound based on the total weight.
보다 구체적으로, 상기 방부 화합물의 사용량은 항균용 조성물의 전체 중량을 기준으로 0.01 내지 50 중량%, 0.01 내지 10 중량%, 0.01 내지 5 중량%, 0.01 내지 1 중량%, 0.05 내지 50 중량%, 0.05 내지 10 중량%, 0.05 내지 5 중량%, 0.05 내지 1 중량%, 0.1 내지 50 중량%, 0.1 내지 10 중량%, 0.1 내지 5 중량%, 0.1 내지 1 중량%, 0.5 내지 50 중량%, 0.5 내지 10 중량%, 0.5 내지 5 중량%, 0.5 내지 1 중량%, 1 내지 50 중량%, 1 내지 10 중량%, 1 내지 5 중량%, 5 내지 50 중량%, 또는 10 내지 50 중량%일 수 있다. More specifically, the amount of the preservative compound used is 0.01 to 50% by weight, 0.01 to 10% by weight, 0.01 to 5% by weight, 0.01 to 1% by weight, 0.05 to 50% by weight, 0.05% by weight, based on the total weight of the antibacterial composition. to 10% by weight, 0.05 to 5% by weight, 0.05 to 1% by weight, 0.1 to 50% by weight, 0.1 to 10% by weight, 0.1 to 5% by weight, 0.1 to 1% by weight, 0.5 to 50% by weight, 0.5 to 10 It may be % by weight, 0.5 to 5% by weight, 0.5 to 1% by weight, 1 to 50% by weight, 1 to 10% by weight, 1 to 5% by weight, 5 to 50% by weight, or 10 to 50% by weight.
상기 방부 화합물의 사용량이 0.01 중량% 미만인 경우에는 세균 및 진균에 대해 항균력이 나타나지 않고 항균력 향상을 위해 많은 양의 루테린 사용이 불가피하며, 상기 방부 화합물의 사용량이 50 중량%를 초과한 경우에는 제품이 묽어져 제형 안정성에 문제가 생기며 피부 자극이 크게 증가할 수 있다.If the amount of the preservative compound used is less than 0.01% by weight, it does not show antibacterial activity against bacteria and fungi, and it is inevitable to use a large amount of reuterin to improve the antibacterial effect. If the amount of the preservative compound used exceeds 50% by weight, the product This dilution may cause problems with formulation stability and significantly increase skin irritation.
또한, 본 발명의 일 구체예에 따르면, 상기 항균용 조성물은 0.05 내지 50 mM의 루테린을 포함하는 것일 수 있다.Additionally, according to one embodiment of the present invention, the antibacterial composition may contain 0.05 to 50 mM of reuterin.
상기 루테린의 농도는 항균용 조성물 중 0.05 내지 50 mM, 0.05 내지 10 mM, 0.05 내지 5 mM, 0.1 내지 50 mM, 0.1 내지 10 mM, 0.1 내지 5 mM, 0.5 내지 50 mM, 0.5 내지 10 mM, 0.5 내지 5 mM, 1 내지 50 mM, 1 내지 10 mM, 또는 1 내지 5 mM일 수 있다.The concentration of reuterin in the antibacterial composition is 0.05 to 50mM, 0.05 to 10mM, 0.05 to 5mM, 0.1 to 50mM, 0.1 to 10mM, 0.1 to 5mM, 0.5 to 50mM, 0.5 to 10mM, It can be 0.5 to 5mM, 1 to 50mM, 1 to 10mM, or 1 to 5mM.
상기 루테린의 농도가 0.05 mM 미만인 경우에는 항균 활성을 나타내지 않거나 일부 미생물에서만 제한적인 항균력을 보일 수 있으며, 상기 루테린의 농도가 50 mM을 초과한 경우에는 제형 안정성에 부정적인 영향을 줄 수 있다.If the concentration of reuterin is less than 0.05mM, it may not show antibacterial activity or may show limited antibacterial activity only to some microorganisms, and if the concentration of reuterin exceeds 50mM, it may have a negative effect on formulation stability.
본 발명의 일 실시예에 따르면, 루테린은 단독 사용으로도 세균 (E. Coli, P. aeruginosa 및 S. aureus)과 진균 (C. albicans 및 A. niger)에 대해 항균력을 나타내지만, 방부 화합물, 즉 하이드록시아세토페논, 글리세릴카프릴레이트, 에틸헥실글리세린 또는 소듐벤조에이트와의 혼용을 통해 루테린 단독 사용에 비해 적은 양만으로도 동일하거나 보다 우수한 항균력을 나타냄을 확인하였다. According to one embodiment of the present invention, lutein exhibits antibacterial activity against bacteria ( E. Coli , P. aeruginosa and S. aureus ) and fungi ( C. albicans and A. niger ) even when used alone, but is a preservative compound. , that is, through mixing with hydroxyacetophenone, glyceryl caprylate, ethylhexylglycerin, or sodium benzoate, it was confirmed that even a small amount showed the same or better antibacterial activity than reuterin alone.
이러한 항균용 조성물은 액상, 유상, 크림상, 페이스트상, 고체상 등 어떠한 제형으로도 응용될 수 있으며, 전신, 피부, 구강, 모발 등 신체에 적용되는 제품에 이용될 수 있다. This antibacterial composition can be applied in any formulation, such as liquid, oil, cream, paste, or solid, and can be used in products applied to the body, such as the whole body, skin, oral cavity, and hair.
본 발명에 따른 항균용 조성물은 사용 목적 또는 양상에 따라 다양한 분야에서 활용될 수 있으며, 일례로 화장료 조성물 또는 이의 첨가제로 구체화될 수 있다. 상기 첨가제는 제품의 미생물에 의한 변질, 부패, 변색 및 화학 변화를 방지하기 위한 첨가물로서 방부제, 보존제, 살균제 등일 수 있으며, 전체 화장료 조성물 중량을 기준으로 0.01 내지 50 중량%, 0.5 내지 25 중량%, 0.1 내지 10 중량%, 또는 0.5 내지 5 중량%가 포함될 수 있다. The antibacterial composition according to the present invention can be used in various fields depending on the purpose or aspect of use, and can be embodied as a cosmetic composition or an additive thereof, for example. The additive is an additive to prevent deterioration, decay, discoloration, and chemical change of the product caused by microorganisms and may be a preservative, preservative, disinfectant, etc., and is contained in an amount of 0.01 to 50% by weight, 0.5 to 25% by weight, based on the total weight of the cosmetic composition. It may contain 0.1 to 10% by weight, or 0.5 to 5% by weight.
본 발명에 따른 항균용 조성물은 화장료 조성물로 구현될 수 있다.The antibacterial composition according to the present invention can be implemented as a cosmetic composition.
본 발명의 일 구체예에 따르면, 상기 화장료 조성물은 항균 성분으로 방부 화합물 및 루테린을 유효성분으로 포함하는 것일 수 있다. According to one embodiment of the present invention, the cosmetic composition may include a preservative compound and reuterin as an antibacterial ingredient as active ingredients.
여기서 방부 화합물 및 루테린에 관한 설명은 상술한 바와 동일하므로, 중복 기재를 피하기 위해 생략한다.Here, the description of the preservative compound and reuterin is the same as described above, so it is omitted to avoid redundant description.
상기 화장료 조성물은 방부 화합물 및 루테린 외에도 화장료 조성물에 통상적으로 사용되는 성분들을 더 포함할 수 있으며, 예를 들면 항산화제, 안정화제, 용해화제, 비타민, 안료, 향료 등을 더 포함할 수 있다.In addition to preservative compounds and reuterin, the cosmetic composition may further include ingredients commonly used in cosmetic compositions, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments, fragrances, etc.
상기 화장료 조성물은 통상 당업계에서 사용되는 제형으로 제한 없이 제조될 수 있으며, 예를 들면, 스킨로션, 스킨 소프너, 스킨토너, 아스트린젠트, 로션, 밀크로션, 모이스처 로션, 영양로션, 마사지크림, 영양크림, 모이스처 크림, 핸드크림, 에센스, 영양에센스, 팩, 비누, 샴푸, 클렌징폼, 클렌징로션, 클렌징크림, 바디로션, 바디클렌저, 유액, 립스틱, 메이크업 베이스, 파운데이션, 프레스파우더, 루스파우더, 아이섀도 등의 제형으로 제조될 수 있다.The cosmetic composition can be prepared without limitation in formulations commonly used in the industry, for example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream. , moisture cream, hand cream, essence, nutritional essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, lipstick, makeup base, foundation, press powder, loose powder, eye shadow. It can be manufactured in a dosage form such as:
본 발명의 일 구체예에 따르면, 상기 화장료 조성물은 전체 중량을 기준으로 방부 화합물 0.01 내지 50 중량%를 포함하는 것일 수 있다.According to one embodiment of the present invention, the cosmetic composition may contain 0.01 to 50% by weight of a preservative compound based on the total weight.
보다 구체적으로, 상기 방부 화합물의 사용량은 화장료 조성물의 전체 중량을 기준으로 0.01 내지 50 중량%, 0.01 내지 10 중량%, 0.01 내지 5 중량%, 0.01 내지 1 중량%, 0.05 내지 50 중량%, 0.05 내지 10 중량%, 0.05 내지 5 중량%, 0.05 내지 1 중량%, 0.1 내지 50 중량%, 0.1 내지 10 중량%, 0.1 내지 5 중량%, 0.1 내지 1 중량%, 0.5 내지 50 중량%, 0.5 내지 10 중량%, 0.5 내지 5 중량%, 0.5 내지 1 중량%, 1 내지 50 중량%, 1 내지 10 중량%, 1 내지 5 중량%, 5 내지 50 중량%, 또는 10 내지 50 중량%일 수 있다. More specifically, the amount of the preservative compound used is 0.01 to 50% by weight, 0.01 to 10% by weight, 0.01 to 5% by weight, 0.01 to 1% by weight, 0.05 to 50% by weight, 0.05 to 0.05% by weight, based on the total weight of the cosmetic composition. 10% by weight, 0.05 to 5% by weight, 0.05 to 1% by weight, 0.1 to 50% by weight, 0.1 to 10% by weight, 0.1 to 5% by weight, 0.1 to 1% by weight, 0.5 to 50% by weight, 0.5 to 10% by weight %, 0.5 to 5% by weight, 0.5 to 1% by weight, 1 to 50% by weight, 1 to 10% by weight, 1 to 5% by weight, 5 to 50% by weight, or 10 to 50% by weight.
또한, 본 발명의 일 구체예에 따르면, 상기 화장료 조성물은 0.05 내지 50 mM의 루테린을 포함하는 것일 수 있다.Additionally, according to one embodiment of the present invention, the cosmetic composition may contain 0.05 to 50 mM of reuterin.
상기 루테린의 농도는 화장료 조성물 중 0.05 내지 50 mM, 0.05 내지 10 mM, 0.05 내지 5 mM, 0.1 내지 50 mM, 0.1 내지 10 mM, 0.1 내지 5 mM, 0.5 내지 50 mM, 0.5 내지 10 mM, 0.5 내지 5 mM, 1 내지 50 mM, 1 내지 10 mM, 또는 1 내지 5 mM일 수 있다.The concentration of reuterin in the cosmetic composition is 0.05 to 50mM, 0.05 to 10mM, 0.05 to 5mM, 0.1 to 50mM, 0.1 to 10mM, 0.1 to 5mM, 0.5 to 50mM, 0.5 to 10mM, 0.5mM to 5mM, 1 to 50mM, 1 to 10mM, or 1 to 5mM.
또한, 본 발명에 따른 항균용 조성물은 화장품 보존용 방부제로 구현될 수 있다.Additionally, the antibacterial composition according to the present invention can be implemented as a preservative for preserving cosmetics.
본 발명의 일 구체예에 따르면, 상기 화장품 보존용 방부제는 방부 화합물 및 루테린을 유효성분으로 포함하는 화장품 보존용 방부제일 수 있다.According to one embodiment of the present invention, the preservative for preserving cosmetics may be a preservative for preserving cosmetics containing a preservative compound and reuterin as active ingredients.
여기서 방부 화합물 및 루테린에 관한 설명은 상술한 바와 동일하므로, 중복 기재를 피하기 위해 생략한다.Here, the description of the preservative compound and reuterin is the same as described above, so it is omitted to avoid redundant description.
상기 화장품 보존용 방부제는 부패를 방지하기 위해 화장품 제조에 사용되는 첨가제로서 특히 생물학적 변질을 예방하는 역할을 한다.The preservative for cosmetics preservation is an additive used in the manufacture of cosmetics to prevent spoilage, and especially plays a role in preventing biological deterioration.
본 발명의 일 구체예에 따르면, 상기 화장품 보존용 방부제는 전체 중량을 기준으로 방부 화합물 0.01 내지 50 중량%를 포함하는 것일 수 있다.According to one embodiment of the present invention, the preservative for preserving cosmetics may contain 0.01 to 50% by weight of a preservative compound based on the total weight.
보다 구체적으로, 상기 방부 화합물의 사용량은 화장품 보존용 방부제의 전체 중량을 기준으로 0.01 내지 50 중량%, 0.01 내지 10 중량%, 0.01 내지 5 중량%, 0.01 내지 1 중량%, 0.05 내지 50 중량%, 0.05 내지 10 중량%, 0.05 내지 5 중량%, 0.05 내지 1 중량%, 0.1 내지 50 중량%, 0.1 내지 10 중량%, 0.1 내지 5 중량%, 0.1 내지 1 중량%, 0.5 내지 50 중량%, 0.5 내지 10 중량%, 0.5 내지 5 중량%, 0.5 내지 1 중량%, 1 내지 50 중량%, 1 내지 10 중량%, 1 내지 5 중량%, 5 내지 50 중량%, 또는 10 내지 50 중량%일 수 있다. More specifically, the amount of the preservative compound used is 0.01 to 50% by weight, 0.01 to 10% by weight, 0.01 to 5% by weight, 0.01 to 1% by weight, 0.05 to 50% by weight, based on the total weight of the preservative for preserving cosmetics. 0.05 to 10% by weight, 0.05 to 5% by weight, 0.05 to 1% by weight, 0.1 to 50% by weight, 0.1 to 10% by weight, 0.1 to 5% by weight, 0.1 to 1% by weight, 0.5 to 50% by weight, 0.5 to 5% by weight It may be 10% by weight, 0.5 to 5% by weight, 0.5 to 1% by weight, 1 to 50% by weight, 1 to 10% by weight, 1 to 5% by weight, 5 to 50% by weight, or 10 to 50% by weight.
또한, 본 발명의 일 구체예에 따르면, 상기 화장품 보존용 방부제는 0.05 내지 50 mM의 루테린을 포함하는 것일 수 있다.Additionally, according to one embodiment of the present invention, the preservative for preserving cosmetics may contain 0.05 to 50 mM of reuterin.
상기 루테린의 농도는 화장품 보존용 방부제 중 0.05 내지 50 mM, 0.05 내지 10 mM, 0.05 내지 5 mM, 0.1 내지 50 mM, 0.1 내지 10 mM, 0.1 내지 5 mM, 0.5 내지 50 mM, 0.5 내지 10 mM, 0.5 내지 5 mM, 1 내지 50 mM, 1 내지 10 mM, 또는 1 내지 5 mM일 수 있다.The concentration of reuterin in the preservative for cosmetics preservation is 0.05 to 50mM, 0.05 to 10mM, 0.05 to 5mM, 0.1 to 50mM, 0.1 to 10mM, 0.1 to 5mM, 0.5 to 50mM, 0.5 to 10mM , 0.5 to 5mM, 1 to 50mM, 1 to 10mM, or 1 to 5mM.
상기 화장품 보존용 방부제는 제품의 종류 및 사용 목적에 따라 통상 사용되는 성분을 더 포함할 수 있으며, 예를 들면, 초산, 초산나트륨계, 소르빈산계, 안식향산계, 프로피온산계 등을 더 포함할 수 있다.The preservative for cosmetics preservation may further include commonly used ingredients depending on the type and purpose of use of the product, for example, acetic acid, sodium acetate, sorbic acid, benzoic acid, propionic acid, etc. .
본 발명에 따른 항균용 조성물은 방부 화합물 및 루테린을 포함함으로써 방부 화합물의 사용량을 최소화하여 제형 안정성을 개선시킬 수 있고 방부 화합물 또는 루테린 단독 사용에 비해 사용량이 감소되어도 제형 내 항균력을 확보할 수 있어 방부 화합물 또는 루테린의 단점을 보완한 화장료 조성물, 화장품 보존용 방부제 등으로 활용될 수 있다.The antibacterial composition according to the present invention can improve formulation stability by minimizing the amount of preservative compound used by containing a preservative compound and reuterin, and can secure antibacterial activity in the formulation even when the amount used is reduced compared to the use of a preservative compound or reuterin alone. Therefore, it can be used as a preservative compound, a cosmetic composition that complements the shortcomings of reuterin, and a preservative for preserving cosmetics.
이하, 본 발명을 보다 상세하게 설명한다. 그러나, 이러한 설명은 본 발명의 이해를 돕기 위하여 예시적으로 제시된 것일 뿐, 본 발명의 범위가 이러한 예시적인 설명에 의하여 제한되는 것은 아니다.Hereinafter, the present invention will be described in more detail. However, this description is merely provided as an example to aid understanding of the present invention, and the scope of the present invention is not limited by this example description.
준비예 1. 방부 화합물 및 루테린 준비Preparation Example 1. Preparation of preservative compound and reuterin
1-1. 방부 화합물1-1. preservative compound
방부 화합물로서 4-하이드록시아세토페논(4-HA), 글리세릴카프릴레이트(GC), 에틸헥실글리세린(EHG) 및 소듐벤조에이트(SB)를 사용하였다. As preservative compounds, 4-hydroxyacetophenone (4-HA), glyceryl caprylate (GC), ethylhexylglycerin (EHG), and sodium benzoate (SB) were used.
1-2. 루테린1-2. reuterin
루테린은 균주 배양을 통해 수득하여 사용하였다. Reuterin was obtained and used through strain culture.
간략하게 설명하면, 5L 배양기에서 락토바실러스 루테리 BR201 균주를 이용하여 MRS 배지 (10 g/L의 프로테오스 펩톤 NO.3, 10 g/L의 쇠고기 추출물, 5 g/L의 효모 추출물, 20 g/L의 덱스트로스, 1 g/L의 폴리솔베이트 80, 2 g/L의 암모늄 시트레이트, 5 g/L의 소듐 아세테이트, 0.1 g/L의 마그네슘 설페이트, 0.05 g/L의 망가니즈 설페이트 및 2 g/L의 디포타슘 설페이트 함유)에서 배양 후 배양액을 13,000 rpm으로 5분간 원심분리한 후 셀을 회수하여 50 mM 소듐 포스페이트 버퍼 (pH 7.5)에 2회 세척하였다. 세척된 셀은 200 mM 글리세롤 용액에 재현탁하여 30℃ 배양기에서 정치반응하며 1시간 간격으로 시료를 채취하였다. 농도가 100 mM인 루테린을 수득하여 이후 실시예에서 사용하였다.Briefly, MRS medium (10 g/L of proteose peptone NO.3, 10 g/L of beef extract, 5 g/L of yeast extract, 20 g) was grown using Lactobacillus reuteri BR201 strain in a 5L culture medium. /L dextrose, 1 g/L polysorbate 80, 2 g/L ammonium citrate, 5 g/L sodium acetate, 0.1 g/L magnesium sulfate, 0.05 g/L manganese sulfate and After culturing in (containing 2 g/L dipotassium sulfate), the culture medium was centrifuged at 13,000 rpm for 5 minutes, and the cells were recovered and washed twice in 50 mM sodium phosphate buffer (pH 7.5). The washed cells were resuspended in 200 mM glycerol solution, incubated in an incubator at 30°C, and samples were collected at 1-hour intervals. Reuterin with a concentration of 100mM was obtained and used in the subsequent examples.
실시예 1. 항균용 조성물의 항균 평가Example 1. Antibacterial evaluation of antibacterial composition
1-1. 항진균 평가 1-1. Antifungal evaluation
진균에 대한 항균력 테스트에서는 미국약전(USP)에서 지정한 진균 Candida albicans ATCC 10231 및 Aspergillus niger ATCC 16404를 이용하였다. C. albicans는 SDB 배지에 2회 계대 배양하여 사용하였고, A. niger는 SDA 배지에 도말하여 수득한 포자를 실험에 사용하였다.In the antibacterial activity test against fungi, the fungi Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404 designated by the United States Pharmacopoeia (USP) were used. C. albicans was subcultured twice in SDB medium, and spores obtained by plating A. niger on SDA medium were used in the experiment.
0.8% NaCl 수용액에 항균용 조성물로서 루테린 단독 또는 루테린과 방부 화합물을 각각의 농도로 첨가하였고, 이때 최종 루테린 농도는 0.5 내지 1.5 mM이었다. 이후 진균을 105 cfu/ml가 되도록 접종하여 30℃에서 3일간 보관 뒤, SDA 배지에 100 uL를 도말하였다. 도말한 시료는 30℃에서 3일간 배양하여 균락(colony) 형성 유무를 확인하였다. 그 결과는 하기 표 1에 나타내었다.Reuterin alone or reuterin and a preservative compound were added at respective concentrations as an antibacterial composition to a 0.8% NaCl aqueous solution, and the final reuterin concentration was 0.5 to 1.5 mM. Afterwards, the fungus was inoculated to a concentration of 10 5 cfu/ml, stored at 30°C for 3 days, and 100 uL was spread on SDA medium. The smeared sample was cultured at 30°C for 3 days to confirm the presence or absence of colonies. The results are shown in Table 1 below.
0.1 wt%4-HA
0.1wt%
0.2 wt%4-HA
0.2wt%
0.1 wt%GC
0.1wt%
0.2 wt%GC
0.2wt%
0.05 wt%EHG
0.05wt%
0.1 wt%EHG
0.1wt%
0.3 wt%SB
0.3wt%
0.4 wt%SB
0.4 wt%
상기 표 1을 참조하면, C. albicans에 대해서는 에틸헥실글리세린 0.05 중량%를 제외하고 하이드록시아세토페논 0.1 ~ 0.2 중량%, 글리세릴카프릴레이트 0.1 ~ 0.2 중량%, 에틸헥실글리세린 0.05 중량% 또는 소듐벤조에이트 0.3 ~ 0.4 중량%를 단독 사용 시 항진균 활성이 없었으나, 농도에 관계없이 루테린을 더 포함할 경우 항진균 활성이 나타나는 것을 확인하였다.Referring to Table 1 above, for C. albicans , excluding 0.05% by weight of ethylhexylglycerin, 0.1 to 0.2% by weight of hydroxyacetophenone, 0.1 to 0.2% by weight of glyceryl caprylate, 0.05% by weight of ethylhexylglycerin, or sodium benzoate. There was no antifungal activity when 0.3 to 0.4% by weight of ate was used alone, but it was confirmed that antifungal activity appeared when additional reuterin was added regardless of the concentration.
또한 A. niger에 대해서는 하이드록시아세토페논 0.1 ~ 0.2 중량%, 글리세릴카프릴레이트 0.1 ~ 0.2 중량%, 에틸헥실글리세린 0.05 ~ 0.1 중량% 또는 소듐벤조에이트 0.3 ~ 0.4 중량%를 단독 사용 시 항진균 활성이 없었으나, 루테린 농도가 증가함에 따라 항진균 활성 또한 증가하는 것을 확인하였다. In addition, against A. niger , antifungal activity was found when 0.1 to 0.2% by weight of hydroxyacetophenone, 0.1 to 0.2% by weight of glyceryl caprylate, 0.05 to 0.1% by weight of ethylhexylglycerin, or 0.3 to 0.4% by weight of sodium benzoate were used alone. However, it was confirmed that the antifungal activity also increased as the reuterin concentration increased.
1-2. 항세균 평가1-2. Antibacterial evaluation
세균에 대한 항균력 테스트에서는 미국약전(USP)에서 지정한 세균 Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027 및 Staphylococcus aureus ATCC 6538를 이용하였다. 세균은 TSB 배지에 2회 계대 배양한 후 실험에 사용하였다.In the antibacterial activity test, bacteria Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, and Staphylococcus aureus ATCC 6538 designated by the United States Pharmacopoeia (USP) were used. The bacteria were subcultured twice in TSB medium and then used in the experiment.
0.8% NaCl 수용액에 항균용 조성물로서 루테린 단독 또는 루테린과 방부 화합물을 각각의 농도로 첨가하였고, 이때 최종 루테린 농도는 0.5 내지 1.5 mM이었다. 이후 세균을 107 cfu/ml가 되도록 접종하여 37℃에서 3일간 보관 뒤, TSA 배지에 100 uL를 도말하였다. 도말한 시료는 37℃에서 배양하여 균락(colony) 형성 유무를 확인하였다. 그 결과는 하기 표 2에 나타내었다.Reuterin alone or reuterin and a preservative compound were added at respective concentrations as an antibacterial composition to a 0.8% NaCl aqueous solution, and the final reuterin concentration was 0.5 to 1.5 mM. Afterwards, bacteria were inoculated at 10 7 cfu/ml, stored at 37°C for 3 days, and 100 uL was plated on TSA medium. The smeared sample was cultured at 37°C to confirm the presence or absence of colonies. The results are shown in Table 2 below.
0.1 wt%4-HA
0.1wt%
0.2 wt%4-HA
0.2wt%
0.1 wt%GC
0.1wt%
0.2 wt%GC
0.2wt%
0.05 wt%EHG
0.05wt%
0.1 wt%EHG
0.1wt%
0.3 wt%SB
0.3wt%
0.4 wt%SB
0.4 wt%
상기 표 2를 참조하면, 각 방부 화합물을 단독 사용한 경우에는 3종의 세균에 대해 항균 활성이 없었으나, 루테린을 첨가할 경우 항세균 활성이 나타나며, 루테린을 단독 사용한 경우에 비해 저농도로 우수한 항세균 활성을 발휘하는 것을 확인하였다.Referring to Table 2 above, when each preservative compound was used alone, there was no antibacterial activity against the three types of bacteria, but when reuterin was added, antibacterial activity was shown, and the antibacterial activity was excellent at a low concentration compared to when reuterin was used alone. It was confirmed that it exerts antibacterial activity.
실시예 2. 화장료 조성물의 제형 안정성 및 항균력 유지 평가Example 2. Evaluation of formulation stability and antibacterial activity maintenance of cosmetic composition
2-1. 화장료 조성물의 제조2-1. Preparation of cosmetic compositions
화장료 조성물에서의 방부 화합물 및/또는 루테린의 항균력을 확인하기 위해 하기 표 3 및 4와 같이 로션 및 시트마스크 제형의 화장료 조성물을 제조하였다.To confirm the antibacterial activity of the preservative compound and/or reuterin in the cosmetic composition, cosmetic compositions in lotion and sheet mask formulations were prepared as shown in Tables 3 and 4 below.
2-2. 화장료 제형에서의 항균력 평가2-2. Evaluation of antibacterial activity in cosmetic formulations
화장료 조성물에서의 항균력 평가를 위해 상기 제형들을 이용하여 테스트를 진행하였다. 항균력 테스트에서는 미국약전(USP)에서 지정한 세균 및 진균을 이용하였다 (Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 6538, Candida albicans ATCC 10231 및 Aspergillus niger ATCC 16404)를 이용하였다. 세균과 C. albicans는 각각 TSB와 SDB 배지에 2회 계대 배양한 후 실험에 사용하였고, A. niger는 SDA 배지에 도말하여 수득한 포자를 실험에 사용하였다.To evaluate the antibacterial activity of cosmetic compositions, tests were conducted using the above formulations. In the antibacterial activity test, bacteria and fungi designated by the United States Pharmacopoeia (USP) were used ( Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 6538, Candida albicans ATCC 10231, and Aspergillus niger ATCC 16404). Bacteria and C. albicans were subcultured twice in TSB and SDB medium, respectively, and then used in the experiment. For A. niger , spores obtained by plating on SDA medium were used in the experiment.
각각의 화장료 조성물에 세균의 경우 107 cfu/ml가, 진균의 경우 105 cfu/ml가 되도록 접종한 후 각각 37℃와 30℃에서 3일간 보관하였다. 100 uL의 시료를 900 uL의 Letheen 배지에 혼합한 뒤 세균의 경우 TSA에, 진균의 경우 SDA에 각각 도말하였다. 도말한 시료는 각각 37℃와 30℃에서 3일간 배양하여 균락(colony) 형성 유무를 확인하였다. 그 결과는 하기 표 5에 나타내었다.Each cosmetic composition was inoculated to a concentration of 10 7 cfu/ml for bacteria and 10 5 cfu/ml for fungi and then stored at 37°C and 30°C for 3 days, respectively. 100 uL of sample was mixed with 900 uL of Letheen medium and then plated on TSA for bacteria and SDA for fungi. The smeared samples were cultured for 3 days at 37°C and 30°C, respectively, to confirm the presence or absence of colonies. The results are shown in Table 5 below.
상기 표 5를 참조하면, 제형에 관계없이 방부 화합물 및 루테린을 포함하는 화장료 조성물은 제형 변화 없이 세균 및 진균에 대해 항균 활성을 가지는 것을 확인하였다.Referring to Table 5, it was confirmed that regardless of the formulation, the cosmetic composition containing a preservative compound and reuterin had antibacterial activity against bacteria and fungi without change in formulation.
2-3. 화장료 제형에서의 항균력 유지 평가2-3. Evaluation of antibacterial activity maintenance in cosmetic formulations
화장료 제형에서의 항균력이 유지되는지 확인하기 위해 '2-2. 화장료 제형에서의 항균력 평가'에서 사용한 세균 또는 진균 접종 시료 (화장료 2 ~ 5 및 7 ~ 10)를 이용하여 각각의 균주를 누적 접종하여 항균력이 유지되는지를 확인하였다. 세균의 경우 3종의 균 (E. coli, P. aeruginosa 및 S. aureus)을 혼합하여 107 CFU/ml이 되도록 접종하였고, 진균의 경우 C. albicans 및 A. niger를 각각 105 CFU/ml이 되도록 접종하였다. 각 균주의 접종은 7일 마다 동일한 시료에 접종을 하였으며, 접종이 완료된 시료는 매 접종 3일차에 100 uL의 시료를 900 uL의 Letheen 배지에 혼합한 뒤 TSA와 SDA에 각각 도말하여 균락 형성 유무를 확인하였다. 그 결과는 하기 표 6에 나타내었다.To check whether the antibacterial activity of the cosmetic formulation is maintained, '2-2. Using the bacterial or fungal inoculation samples (cosmetics 2 to 5 and 7 to 10) used in 'Evaluation of antibacterial activity in cosmetic formulations', each strain was cumulatively inoculated to confirm whether the antibacterial activity was maintained. In the case of bacteria, three types of bacteria ( E. coli , P. aeruginosa , and S. aureus ) were mixed and inoculated to make 10 7 CFU/ml, and in the case of fungi, C. albicans and A. niger were inoculated at 10 5 CFU/ml each. It was inoculated to achieve this. Each strain was inoculated into the same sample every 7 days, and on the 3rd day of each inoculation, 100 uL of the sample was mixed with 900 uL of Letheen medium and smeared on TSA and SDA respectively to determine the presence or absence of bacterial colonies. Confirmed. The results are shown in Table 6 below.
상기 표 6을 참조하면, 제형에 관계없이 방부 화합물 및 루테린을 포함하는 화장료 조성물은 제형 변화 없이 세균 및 진균에 대해 항균 활성이 안정적으로 지속되는 것을 확인하였다.Referring to Table 6, it was confirmed that regardless of the formulation, the cosmetic composition containing a preservative compound and reuterin stably maintained antibacterial activity against bacteria and fungi without change in formulation.
이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.So far, the present invention has been examined focusing on its preferred embodiments. A person skilled in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a restrictive perspective. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the equivalent scope should be construed as being included in the present invention.
Claims (7)
상기 방부 화합물은 하이드록시아세토페논, 글리세릴카프릴레이트, 에틸헥실글리세린 및 소듐벤조에이트로 이루어진 군에서 선택된 1종 이상인 것인 항균용 조성물.In claim 1,
The antibacterial composition is one or more selected from the group consisting of hydroxyacetophenone, glyceryl caprylate, ethylhexylglycerin, and sodium benzoate.
상기 항균용 조성물은 세균 및 진균에 대해 항균 활성을 가지는 것인 항균용 조성물.In claim 1,
The antibacterial composition has antibacterial activity against bacteria and fungi.
상기 항균용 조성물은 전체 중량을 기준으로 방부 화합물 0.01 내지 50 중량%를 포함하는 것인 항균용 조성물.In claim 1,
The antibacterial composition includes 0.01 to 50% by weight of a preservative compound based on the total weight.
상기 항균용 조성물은 0.05 내지 50 mM의 루테린을 포함하는 것인 항균용 조성물.In claim 1,
The antibacterial composition is an antibacterial composition containing 0.05 to 50mM of reuterin.
Priority Applications (2)
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| KR1020220034592A KR20230137014A (en) | 2022-03-21 | 2022-03-21 | Antimicrobial composition comprising preservative compound and reuterin and use thereof |
| PCT/KR2022/014266 WO2023075165A1 (en) | 2021-10-26 | 2022-09-23 | Antimicrobial composition comprising antiseptic compound and reuterin, and use thereof |
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| KR1020220034592A KR20230137014A (en) | 2022-03-21 | 2022-03-21 | Antimicrobial composition comprising preservative compound and reuterin and use thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101472518B1 (en) | 2011-12-27 | 2014-12-17 | 주식회사 제닉 | Composition of preservatives for cosmetic and cosmetic composition comprising the same |
| KR102013565B1 (en) | 2017-12-05 | 2019-08-23 | 한국생명공학연구원 | Lactobacillus reuteri OH0335 strain having high productivity of reuterin from glycerol and uses thereof |
| KR102136623B1 (en) | 2018-06-18 | 2020-07-23 | 스킨큐어 주식회사 | An anti bacterial composition comprising plant extracts and the complex antiseptic compositions comprising the same |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101472518B1 (en) | 2011-12-27 | 2014-12-17 | 주식회사 제닉 | Composition of preservatives for cosmetic and cosmetic composition comprising the same |
| KR102013565B1 (en) | 2017-12-05 | 2019-08-23 | 한국생명공학연구원 | Lactobacillus reuteri OH0335 strain having high productivity of reuterin from glycerol and uses thereof |
| KR102136623B1 (en) | 2018-06-18 | 2020-07-23 | 스킨큐어 주식회사 | An anti bacterial composition comprising plant extracts and the complex antiseptic compositions comprising the same |
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