KR20230101354A - Sesame bath extract with increased sesaminol content and use thereof - Google Patents
Sesame bath extract with increased sesaminol content and use thereof Download PDFInfo
- Publication number
- KR20230101354A KR20230101354A KR1020210191388A KR20210191388A KR20230101354A KR 20230101354 A KR20230101354 A KR 20230101354A KR 1020210191388 A KR1020210191388 A KR 1020210191388A KR 20210191388 A KR20210191388 A KR 20210191388A KR 20230101354 A KR20230101354 A KR 20230101354A
- Authority
- KR
- South Korea
- Prior art keywords
- sesame
- bath
- extract
- bath extract
- increased
- Prior art date
Links
- 235000003434 Sesamum indicum Nutrition 0.000 title claims abstract description 142
- 239000000284 extract Substances 0.000 title claims abstract description 115
- KQRXQIPRDKVZPW-ISZNXKAUSA-N sesaminol Chemical compound C1=C2OCOC2=CC([C@H]2OC[C@H]3[C@@H]2CO[C@@H]3C2=CC=3OCOC=3C=C2O)=C1 KQRXQIPRDKVZPW-ISZNXKAUSA-N 0.000 title claims abstract description 37
- KQRXQIPRDKVZPW-UHFFFAOYSA-N sesaminol Natural products C1=C2OCOC2=CC(C2OCC3C2COC3C2=CC=3OCOC=3C=C2O)=C1 KQRXQIPRDKVZPW-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 230000001965 increasing effect Effects 0.000 title claims abstract description 29
- 244000000231 Sesamum indicum Species 0.000 title description 131
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 52
- 230000002087 whitening effect Effects 0.000 claims abstract description 42
- 239000000203 mixture Substances 0.000 claims abstract description 41
- 230000037303 wrinkles Effects 0.000 claims abstract description 29
- 230000006872 improvement Effects 0.000 claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 claims abstract description 20
- 239000002537 cosmetic Substances 0.000 claims abstract description 19
- 239000004480 active ingredient Substances 0.000 claims abstract description 14
- 235000013376 functional food Nutrition 0.000 claims abstract description 12
- 230000036541 health Effects 0.000 claims abstract description 11
- 238000003287 bathing Methods 0.000 claims abstract description 10
- 241000207961 Sesamum Species 0.000 claims abstract 12
- -1 body cleanser Substances 0.000 claims description 21
- 235000013305 food Nutrition 0.000 claims description 14
- 239000006210 lotion Substances 0.000 claims description 12
- 239000006071 cream Substances 0.000 claims description 11
- 238000009472 formulation Methods 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 230000003796 beauty Effects 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 239000000686 essence Substances 0.000 claims description 2
- 239000006260 foam Substances 0.000 claims description 2
- 239000008269 hand cream Substances 0.000 claims description 2
- 235000013336 milk Nutrition 0.000 claims description 2
- 239000008267 milk Substances 0.000 claims description 2
- 210000004080 milk Anatomy 0.000 claims description 2
- 239000000344 soap Substances 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 claims 1
- 230000035764 nutrition Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 20
- 239000000463 material Substances 0.000 abstract description 2
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 36
- 210000003491 skin Anatomy 0.000 description 33
- 150000001875 compounds Chemical class 0.000 description 31
- 102000003425 Tyrosinase Human genes 0.000 description 30
- 108060008724 Tyrosinase Proteins 0.000 description 30
- 230000002401 inhibitory effect Effects 0.000 description 27
- 102100021657 Tyrosine-protein phosphatase non-receptor type 6 Human genes 0.000 description 22
- 101710128901 Tyrosine-protein phosphatase non-receptor type 6 Proteins 0.000 description 22
- 102000000380 Matrix Metalloproteinase 1 Human genes 0.000 description 21
- 108010016113 Matrix Metalloproteinase 1 Proteins 0.000 description 21
- 102000004169 proteins and genes Human genes 0.000 description 19
- 108090000623 proteins and genes Proteins 0.000 description 19
- 238000002360 preparation method Methods 0.000 description 13
- 239000013641 positive control Substances 0.000 description 12
- 235000018102 proteins Nutrition 0.000 description 12
- 239000000523 sample Substances 0.000 description 12
- 101800001751 Melanocyte-stimulating hormone alpha Proteins 0.000 description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 102100027467 Pro-opiomelanocortin Human genes 0.000 description 9
- 108010050808 Procollagen Proteins 0.000 description 9
- 238000002835 absorbance Methods 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 230000036564 melanin content Effects 0.000 description 9
- 230000037333 procollagen synthesis Effects 0.000 description 9
- WHNFPRLDDSXQCL-UAZQEYIDSA-N α-msh Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(N)=O)NC(=O)[C@H](CO)NC(C)=O)C1=CC=C(O)C=C1 WHNFPRLDDSXQCL-UAZQEYIDSA-N 0.000 description 9
- WMBWREPUVVBILR-WIYYLYMNSA-N (-)-Epigallocatechin-3-o-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-WIYYLYMNSA-N 0.000 description 8
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
- 102000029816 Collagenase Human genes 0.000 description 8
- 108060005980 Collagenase Proteins 0.000 description 8
- WMBWREPUVVBILR-UHFFFAOYSA-N GCG Natural products C=1C(O)=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-UHFFFAOYSA-N 0.000 description 8
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 8
- 229960002424 collagenase Drugs 0.000 description 8
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 8
- 238000005259 measurement Methods 0.000 description 8
- 239000002904 solvent Substances 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- 102100030310 5,6-dihydroxyindole-2-carboxylic acid oxidase Human genes 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 7
- 102100031413 L-dopachrome tautomerase Human genes 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 230000001153 anti-wrinkle effect Effects 0.000 description 7
- 108010051081 dopachrome isomerase Proteins 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 230000002829 reductive effect Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 108010014402 tyrosinase-related protein-1 Proteins 0.000 description 7
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000006180 TBST buffer Substances 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- 239000000796 flavoring agent Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 5
- 102000013760 Microphthalmia-Associated Transcription Factor Human genes 0.000 description 5
- 108010050345 Microphthalmia-Associated Transcription Factor Proteins 0.000 description 5
- 235000013361 beverage Nutrition 0.000 description 5
- 235000014113 dietary fatty acids Nutrition 0.000 description 5
- 229940088598 enzyme Drugs 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 239000000194 fatty acid Substances 0.000 description 5
- 229930195729 fatty acid Natural products 0.000 description 5
- 230000008099 melanin synthesis Effects 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 229960000271 arbutin Drugs 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 231100000135 cytotoxicity Toxicity 0.000 description 4
- 230000003013 cytotoxicity Effects 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 235000013355 food flavoring agent Nutrition 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 230000003834 intracellular effect Effects 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 4
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 3
- YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical group C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 3
- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 229910002091 carbon monoxide Inorganic materials 0.000 description 3
- 235000014171 carbonated beverage Nutrition 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 229940030275 epigallocatechin gallate Drugs 0.000 description 3
- 235000019253 formic acid Nutrition 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 235000011187 glycerol Nutrition 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000004017 serum-free culture medium Substances 0.000 description 3
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 238000009010 Bradford assay Methods 0.000 description 2
- 241000675108 Citrus tangerina Species 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 2
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 2
- 238000008157 ELISA kit Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- UIOFUWFRIANQPC-JKIFEVAISA-N Floxacillin Chemical compound N([C@@H]1C(N2[C@H](C(C)(C)S[C@@H]21)C(O)=O)=O)C(=O)C1=C(C)ON=C1C1=C(F)C=CC=C1Cl UIOFUWFRIANQPC-JKIFEVAISA-N 0.000 description 2
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 2
- 231100000002 MTT assay Toxicity 0.000 description 2
- 238000000134 MTT assay Methods 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 238000005481 NMR spectroscopy Methods 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 239000002033 PVDF binder Substances 0.000 description 2
- 229920002230 Pectic acid Polymers 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 2
- LUSZGTFNYDARNI-UHFFFAOYSA-N Sesamol Natural products OC1=CC=C2OCOC2=C1 LUSZGTFNYDARNI-UHFFFAOYSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- 239000013504 Triton X-100 Substances 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 235000010724 Wisteria floribunda Nutrition 0.000 description 2
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 2
- 239000000783 alginic acid Substances 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- 229960001126 alginic acid Drugs 0.000 description 2
- 150000004781 alginic acids Chemical class 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000000440 bentonite Substances 0.000 description 2
- 229910000278 bentonite Inorganic materials 0.000 description 2
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 2
- SESFRYSPDFLNCH-UHFFFAOYSA-N benzyl benzoate Chemical compound C=1C=CC=CC=1C(=O)OCC1=CC=CC=C1 SESFRYSPDFLNCH-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 238000002038 chemiluminescence detection Methods 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 235000019439 ethyl acetate Nutrition 0.000 description 2
- 239000002038 ethyl acetate fraction Substances 0.000 description 2
- 230000001747 exhibiting effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 235000012041 food component Nutrition 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 229940093915 gynecological organic acid Drugs 0.000 description 2
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 2
- 229960004705 kojic acid Drugs 0.000 description 2
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 201000001441 melanoma Diseases 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- LCLHHZYHLXDRQG-ZNKJPWOQSA-N pectic acid Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)O[C@H](C(O)=O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](OC2[C@@H]([C@@H](O)[C@@H](O)[C@H](O2)C(O)=O)O)[C@@H](C(O)=O)O1 LCLHHZYHLXDRQG-ZNKJPWOQSA-N 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000010318 polygalacturonic acid Substances 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 239000012064 sodium phosphate buffer Substances 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 235000010356 sorbitol Nutrition 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 239000000454 talc Substances 0.000 description 2
- 229910052623 talc Inorganic materials 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000002562 thickening agent Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- PEYUIKBAABKQKQ-AFHBHXEDSA-N (+)-sesamin Chemical compound C1=C2OCOC2=CC([C@H]2OC[C@H]3[C@@H]2CO[C@@H]3C2=CC=C3OCOC3=C2)=C1 PEYUIKBAABKQKQ-AFHBHXEDSA-N 0.000 description 1
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- 150000005208 1,4-dihydroxybenzenes Chemical class 0.000 description 1
- WNWHHMBRJJOGFJ-UHFFFAOYSA-N 16-methylheptadecan-1-ol Chemical class CC(C)CCCCCCCCCCCCCCCO WNWHHMBRJJOGFJ-UHFFFAOYSA-N 0.000 description 1
- XHSWVNQODRKABJ-UHFFFAOYSA-N 2,7-dinitroindazole Chemical compound [O-][N+](=O)C1=CC=CC2=CN([N+]([O-])=O)N=C12 XHSWVNQODRKABJ-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- FTZIQBGFCYJWKA-UHFFFAOYSA-N 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Chemical compound S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 FTZIQBGFCYJWKA-UHFFFAOYSA-N 0.000 description 1
- AZKSAVLVSZKNRD-UHFFFAOYSA-M 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chemical compound [Br-].S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 AZKSAVLVSZKNRD-UHFFFAOYSA-M 0.000 description 1
- VOUAQYXWVJDEQY-QENPJCQMSA-N 33017-11-7 Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N1[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)CCC1 VOUAQYXWVJDEQY-QENPJCQMSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 description 1
- 241001116389 Aloe Species 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 108010011485 Aspartame Proteins 0.000 description 1
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 108010075254 C-Peptide Proteins 0.000 description 1
- 101100456566 Caenorhabditis elegans dpy-22 gene Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- AFSDNFLWKVMVRB-UHFFFAOYSA-N Ellagic acid Chemical compound OC1=C(O)C(OC2=O)=C3C4=C2C=C(O)C(O)=C4OC(=O)C3=C1 AFSDNFLWKVMVRB-UHFFFAOYSA-N 0.000 description 1
- ATJXMQHAMYVHRX-CPCISQLKSA-N Ellagic acid Natural products OC1=C(O)[C@H]2OC(=O)c3cc(O)c(O)c4OC(=O)C(=C1)[C@H]2c34 ATJXMQHAMYVHRX-CPCISQLKSA-N 0.000 description 1
- 229920002079 Ellagic acid Polymers 0.000 description 1
- 206010014970 Ephelides Diseases 0.000 description 1
- 239000004386 Erythritol Substances 0.000 description 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241001245789 Goodea atripinnis Species 0.000 description 1
- 206010019133 Hangover Diseases 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 108010028554 LDL Cholesterol Proteins 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 239000004166 Lanolin Substances 0.000 description 1
- 241000220225 Malus Species 0.000 description 1
- 235000011430 Malus pumila Nutrition 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 240000001987 Pyrus communis Species 0.000 description 1
- YIQKLZYTHXTDDT-UHFFFAOYSA-H Sirius red F3B Chemical compound C1=CC(=CC=C1N=NC2=CC(=C(C=C2)N=NC3=C(C=C4C=C(C=CC4=C3[O-])NC(=O)NC5=CC6=CC(=C(C(=C6C=C5)[O-])N=NC7=C(C=C(C=C7)N=NC8=CC=C(C=C8)S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)O)S(=O)(=O)O)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+] YIQKLZYTHXTDDT-UHFFFAOYSA-H 0.000 description 1
- 206010040799 Skin atrophy Diseases 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 244000228451 Stevia rebaudiana Species 0.000 description 1
- ULUAUXLGCMPNKK-UHFFFAOYSA-N Sulfobutanedioic acid Chemical compound OC(=O)CC(C(O)=O)S(O)(=O)=O ULUAUXLGCMPNKK-UHFFFAOYSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 240000008866 Ziziphus nummularia Species 0.000 description 1
- IKHGUXGNUITLKF-XPULMUKRSA-N acetaldehyde Chemical compound [14CH]([14CH3])=O IKHGUXGNUITLKF-XPULMUKRSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000013334 alcoholic beverage Nutrition 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 235000011399 aloe vera Nutrition 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000012223 aqueous fraction Substances 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 239000000605 aspartame Substances 0.000 description 1
- 235000010357 aspartame Nutrition 0.000 description 1
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 description 1
- 229960003438 aspartame Drugs 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 229960002903 benzyl benzoate Drugs 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000001273 butane Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000000378 calcium silicate Substances 0.000 description 1
- 229910052918 calcium silicate Inorganic materials 0.000 description 1
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000005827 chlorofluoro hydrocarbons Chemical class 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229940097362 cyclodextrins Drugs 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- MHUWZNTUIIFHAS-CLFAGFIQSA-N dioleoyl phosphatidic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(COP(O)(O)=O)OC(=O)CCCCCCC\C=C/CCCCCCCC MHUWZNTUIIFHAS-CLFAGFIQSA-N 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- VJNCICVKUHKIIV-UHFFFAOYSA-N dopachrome Chemical compound O=C1C(=O)C=C2NC(C(=O)O)CC2=C1 VJNCICVKUHKIIV-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
- 229960002852 ellagic acid Drugs 0.000 description 1
- 235000004132 ellagic acid Nutrition 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- PEYUIKBAABKQKQ-UHFFFAOYSA-N epiasarinin Natural products C1=C2OCOC2=CC(C2OCC3C2COC3C2=CC=C3OCOC3=C2)=C1 PEYUIKBAABKQKQ-UHFFFAOYSA-N 0.000 description 1
- 235000019414 erythritol Nutrition 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 229940009714 erythritol Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 229930003949 flavanone Natural products 0.000 description 1
- 150000002208 flavanones Chemical class 0.000 description 1
- 235000011981 flavanones Nutrition 0.000 description 1
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Chemical class O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000005570 heteronuclear single quantum coherence Methods 0.000 description 1
- 239000002044 hexane fraction Substances 0.000 description 1
- 238000002114 high-resolution electrospray ionisation mass spectrometry Methods 0.000 description 1
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 239000003752 hydrotrope Substances 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- MTNDZQHUAFNZQY-UHFFFAOYSA-N imidazoline Chemical class C1CN=CN1 MTNDZQHUAFNZQY-UHFFFAOYSA-N 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- SUMDYPCJJOFFON-UHFFFAOYSA-N isethionic acid Chemical compound OCCS(O)(=O)=O SUMDYPCJJOFFON-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 235000019388 lanolin Nutrition 0.000 description 1
- 229940039717 lanolin Drugs 0.000 description 1
- 229960004502 levodopa Drugs 0.000 description 1
- 229940069445 licorice extract Drugs 0.000 description 1
- 229930013686 lignan Natural products 0.000 description 1
- 235000009408 lignans Nutrition 0.000 description 1
- 150000005692 lignans Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 description 1
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 1
- 239000008108 microcrystalline cellulose Substances 0.000 description 1
- 229940016286 microcrystalline cellulose Drugs 0.000 description 1
- CQDGTJPVBWZJAZ-UHFFFAOYSA-N monoethyl carbonate Chemical compound CCOC(O)=O CQDGTJPVBWZJAZ-UHFFFAOYSA-N 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 1
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 1
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 235000013550 pizza Nutrition 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000001294 propane Substances 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 238000000425 proton nuclear magnetic resonance spectrum Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000003642 reactive oxygen metabolite Substances 0.000 description 1
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 1
- 235000019204 saccharin Nutrition 0.000 description 1
- 229940081974 saccharin Drugs 0.000 description 1
- 239000000901 saccharin and its Na,K and Ca salt Substances 0.000 description 1
- 229940071089 sarcosinate Drugs 0.000 description 1
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 210000002374 sebum Anatomy 0.000 description 1
- 230000037307 sensitive skin Effects 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- VRMHCMWQHAXTOR-CMOCDZPBSA-N sesamin Natural products C1=C2OCOC2=CC([C@@H]2OC[C@@]3(C)[C@H](C=4C=C5OCOC5=CC=4)OC[C@]32C)=C1 VRMHCMWQHAXTOR-CMOCDZPBSA-N 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 210000001626 skin fibroblast Anatomy 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 235000011888 snacks Nutrition 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229960002920 sorbitol Drugs 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 230000000475 sunscreen effect Effects 0.000 description 1
- 239000000516 sunscreening agent Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 229940104261 taurate Drugs 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 239000000892 thaumatin Substances 0.000 description 1
- 235000010436 thaumatin Nutrition 0.000 description 1
- 235000015193 tomato juice Nutrition 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000002495 two-dimensional nuclear magnetic resonance spectrum Methods 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 230000037331 wrinkle reduction Effects 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
본 발명은 세사미놀 함량이 증진된 참깨 중탕 추출물 및 상기 참깨 중탕 추출물을 유효성분으로 함유하는 미백 및 주름 개선용 조성물에 관한 것으로, 참깨에 물을 첨가한 다음 pH를 2~6으로 맞춘 후, 밀폐된 용기에서 50~160℃의 온도 조건으로 10~200분 동안 중탕하는 단계를 포함하는 본 발명의 제조방법으로 제조된 참깨 중탕 추출물은 직접적으로 열을 가해 추출한 참깨 열수 추출물에 비해 미백 및 주름 개선 효과가 현저히 증진되는 효과가 있으므로, 본 발명의 제조방법으로 제조된 참깨 중탕 추출물은 피부 미백 및 주름 개선용 화장품 또는 건강기능식품의 소재로 유용하게 사용될 수 있다.The present invention relates to a sesame bath-bath extract with increased sesaminol content and a whitening and wrinkle-improving composition containing the sesame bath-bath extract as an active ingredient. The sesame bath extract prepared by the manufacturing method of the present invention, which includes the step of bathing in a water bath at a temperature of 50 to 160 ° C. for 10 to 200 minutes in a container, has a whitening and wrinkle improvement effect compared to a sesame hot water extract extracted by directly applying heat. Since it has an effect of significantly enhancing, the sesame bath extract prepared by the manufacturing method of the present invention can be usefully used as a material for cosmetics or health functional food for skin whitening and wrinkle improvement.
Description
본 발명은 세사미놀 함량이 증진된 참깨 중탕 추출물 및 상기 참깨 중탕 추출물을 유효성분으로 함유하는 피부 미백 및 주름 개선용 조성물에 관한 것이다.The present invention relates to a sesame bath-bath extract with increased sesaminol content and a composition for skin whitening and wrinkle improvement containing the sesame bath-bath extract as an active ingredient.
참깨(Sesamum indicum L.)는 참깨과 참깨속에 속하는 1년생 초본으로 열대에서 냉온대에 걸쳐 재배되는 세계적인 유료작물이다.Sesame ( Sesamum indicum L. ) is an annual herb belonging to the Sesame family Sesame genus and is a world-class oil crop cultivated in the tropics to cool and temperate zones.
참깨의 영양학적 성분을 살펴보면 지질 51%, 단백질 20%, 당질 15% 정도를 함유하고 있으며, 쌀과 소맥에 비하여 칼슘, 인, 아연, 철, 비타민 B1, B2, 불포화지방산 등이 풍부한 식품이다. 이와 더불어, 참깨에는 항산화 효과를 나타내는 세사민(sesamin) 0.1~1.0%와 세사미놀(sesaminol)이 0.3~0.7% 함유되어 있다.Looking at the nutritional components of sesame seeds, they contain 51% lipid, 20% protein, and 15% sugar, and are rich in calcium, phosphorus, zinc, iron, vitamins B1, B2, and unsaturated fatty acids compared to rice and wheat. In addition, sesame seeds contain 0.1 to 1.0% of sesamin and 0.3 to 0.7% of sesaminol, which exhibit antioxidant effects.
그 중 세사미놀은 참깨에 들어있는 지용성 리그난 성분으로 세사몰 성분의 전구체이다. 강력한 항산화 작용을 가지고 있어 체내와 식품 속에 지질이 산화되어 생성되는 과산화 지질의 생성과 동맥경화의 원인이 되는 나쁜 콜레스테롤인 LDL이 생성되는 것을 방지하며 숙취의 원인인 아세트알데하이드의 분해속도를 빠르게 하는 효과가 있다.Among them, sesaminol is a fat-soluble lignan component contained in sesame seeds and is a precursor of sesamol component. It has a strong antioxidant effect, which prevents the formation of lipid peroxide, which is produced by oxidation of lipids in the body and food, and the formation of bad cholesterol, LDL, which causes arteriosclerosis, and speeds up the decomposition rate of acetaldehyde, which is the cause of hangovers. there is
한편, 피부는 외부 자극으로부터 우리 몸을 보호해주는 인체 최전방의 방어 장기이며, 미적인 관점에서도 그 관심이 점점 높아지고 있다. 이에 따라 다양한 기능성 화장품에 대한 연구가 활발한데, 그 중에서도 주름 완화 및 미백 기능에 대한 다양한 기능성 화장품들이 출시되고 있다.On the other hand, the skin is the foremost defense organ of the human body that protects our body from external stimuli, and interest in it is gradually increasing from an aesthetic point of view. Accordingly, research on various functional cosmetics is active, and among them, various functional cosmetics for wrinkle reduction and whitening functions are being released.
주름의 생성 원인으로는 여러 가지가 보고되고 있는데, 우선 피부가 과다한 자외선에 노출되면 피부 구성 성분인 콜라겐(collagen)의 분해가 촉진되어 피부가 탄력을 잃고 주름이 생성될 수 있다. 또한, 피부가 과도한 온도 변화 및 습도 저하나 바람 등에 의해 지나치게 건조해지면 외부에 대한 방어벽으로서의 피부 기능이 저하되어 주름이 생기기도 한다. 그리고, 피부가 활성 산소종이나 자유 라디칼에 노출되면 산화 작용에 의해 과산화 지질이 생성되고 그로 인해 콜라겐 등의 피부 구성 단백질이 변형되어 주름이 생성될 수 있다. 이러한 외부적 요인과 내부적 요인에 의한 주름 발생을 억제하고자 다양한 화장품 조성물이 연구되고 있는 실정이다.Various causes of wrinkles have been reported. First, when the skin is exposed to excessive ultraviolet rays, the decomposition of collagen, a component of the skin, is promoted, resulting in loss of skin elasticity and formation of wrinkles. In addition, when the skin becomes excessively dry due to excessive temperature changes, low humidity, wind, etc., the function of the skin as a barrier against the outside deteriorates, resulting in wrinkles. In addition, when the skin is exposed to reactive oxygen species or free radicals, lipid peroxide is produced by oxidation, and as a result, skin constituent proteins such as collagen are modified to form wrinkles. In order to suppress the occurrence of wrinkles caused by these external and internal factors, various cosmetic compositions are being studied.
또한, 피부에 자외선이 조사되면 피부 중 멜라노사이트(melanocyte)가 활성화되어 티로시나아제(tyrosinase)와 TRP1(tyrosinase related protein 1) 및 TRP2(tyrosinase related protein 2)의 작용에 의해 멜라닌 생성이 촉진된다. 생성된 멜라닌은 피부에 침착되어 검버섯 또는 주근깨로 발전하는데, 이것을 방지하기 위하여 다양한 조성물이 함유된 미백 화장품이 사용되고 있다.In addition, when the skin is irradiated with ultraviolet rays, melanocytes in the skin are activated, and melanin production is promoted by the action of tyrosinase, TRP1 (tyrosinase related protein 1) and TRP2 (tyrosinase related protein 2). The produced melanin is deposited on the skin and develops into age spots or freckles. In order to prevent this, whitening cosmetics containing various compositions are used.
자외선은 피지, 세포막 등의 산화를 촉진하여 각종 피부장애를 일으키는 것으로 밝혀지고 있으며, 특히 최근 오존층 파괴에 따른 자외선의 증가로 보다 효과적인 피부 산화 방지책이 요구되는 실정이다.It has been found that ultraviolet rays promote oxidation of sebum and cell membranes to cause various skin disorders. In particular, with the recent increase in ultraviolet rays due to the destruction of the ozone layer, more effective measures to prevent skin oxidation are required.
종래 알려진 미백 효과를 나타내는 성분은 아스코르브산, 인산 에스테르염, 하이드로퀴논 유도체, 태반 추출물, 코지산, 엘라그산 등이 있으며, 이들 성분을 배합한 화장품 조성물이 일반적이다. 최근에는 식물에 함유된 폴리페놀 등에 높은 미백 효과가 있는 것으로 보고되어 있으며, 이를 사용하는 화장품 조성물이 제안되고 있다. 또한, 플라바논류(flavanones) 및 하이드록시플라본류(hydroxyflavones)의 미백 효과에 대해서도 이미 개시되어 있다. 그러나, 이들 미백 성분은 그 효과가 다소 미비하고 보존시 문제점이 있으며, 미백 효과 이외에 주름 완화 효과 및 노화 방지 효과를 충분히 발휘하지 못하는 실정이다. 특히 화학 성분이 함유된 기능성 화장품의 경우 피부 자극을 유발하여 민감성 피부에 적합하지 않은 문제점이 있는바, 피부에 자극이 적고 친환경적인 천연 식물 유래의 기능성 화장품에 대한 관심이 집중되고 있다.Conventionally known components exhibiting a whitening effect include ascorbic acid, phosphate ester salts, hydroquinone derivatives, placenta extracts, kojic acid, ellagic acid, and the like, and cosmetic compositions containing these components are common. Recently, polyphenols contained in plants have been reported to have a high whitening effect, and cosmetic compositions using them have been proposed. In addition, the whitening effect of flavanones and hydroxyflavones has already been disclosed. However, these whitening ingredients are somewhat insignificant in their effects and have problems during preservation, and do not sufficiently exhibit wrinkle alleviating effects and anti-aging effects in addition to the whitening effects. In particular, in the case of functional cosmetics containing chemical components, there is a problem that causes skin irritation and is not suitable for sensitive skin, so attention is focused on functional cosmetics derived from natural plants that are less irritating to the skin and are eco-friendly.
한편, 한국등록특허 제0239206호에는 참깨유와 참깨박으로부터 세사미놀 성분을 분리 및 정제하는 방법이 개시되어 있고, 한국공개특허 제2018-0064848호에는 백수오 추출물 유래의 생리활성 물질을 포함하는 피부 미백 및 주름 개선용 조성물이 개시되어 있으며, 한국공개특허 제2021-0044952호에는 대추씨 발효물을 유효성분으로 포함하는 피부 미백 및 주름 개선용 조성물이 개시되어 있지만, 본 발명의 세사미놀 함량이 증진된 참깨 중탕 추출물 및 상기 참깨 중탕 추출물을 유효성분으로 함유하는 피부 미백 및 주름 개선용 조성물에 관해 전혀 개시된 바 없다. On the other hand, Korean Patent Registration No. 0239206 discloses a method for separating and purifying sesaminol components from sesame oil and sesame meal, and Korean Patent Publication No. 2018-0064848 discloses skin containing physiologically active substances derived from Baeksuo extract. A composition for whitening and improving wrinkles is disclosed, and Korean Patent Publication No. 2021-0044952 discloses a composition for skin whitening and wrinkle improvement containing fermented jujube seeds as an active ingredient, but the sesaminol content of the present invention is enhanced. There is no disclosure of a sesame bath extract and a composition for skin whitening and wrinkle improvement containing the sesame bath extract as an active ingredient.
본 발명은 상기와 같은 요구에 의해 도출된 것으로, 참깨에 물을 첨가한 다음 pH를 2~6으로 맞춘 후, 밀폐된 용기에서 50~160℃의 온도 조건으로 10~200분 동안 중탕하는 단계를 포함하는 세사미놀 함량이 증진된 참깨 중탕 추출물의 제조방법을 제공하고, 상기 제조방법으로 제조된 참깨 중탕 추출물의 미백 및 주름 개선 효과가 직접적인 열을 가해 추출한 참깨 열수 추출물에 비해 현저히 증진되는 것을 확인함으로써, 본 발명을 완성하였다.The present invention was derived from the above request, and after adding water to sesame seeds, adjusting the pH to 2 to 6, and then heating in a water bath for 10 to 200 minutes at a temperature of 50 to 160 ° C. in a sealed container By providing a method for producing a sesame bath extract containing increased sesaminol content, and confirming that the whitening and wrinkle-improving effects of the sesame bath extract prepared by the manufacturing method are significantly improved compared to the sesame hot water extract extracted by applying direct heat. , completed the present invention.
상기 과제를 해결하기 위하여, 본 발명은In order to solve the above problems, the present invention
1) 세척된 참깨에 물을 첨가한 후, pH를 2~6으로 조정하는 단계;1) adding water to the washed sesame seeds and adjusting the pH to 2-6;
2) 상기 단계 1) 이후에, 50~160℃의 온도 조건으로 10~200분 동안 중탕하는 단계; 및2) after step 1), bathing in hot water for 10 to 200 minutes at a temperature of 50 to 160 ° C; and
3) 상기 단계 2) 이후에, 여과하는 단계;를 포함하는 세사미놀 함량이 증진된 참깨 중탕 추출물의 제조방법을 제공한다.3) after step 2), filtering; provides a method for producing a sesame bath extract having increased sesaminol content.
또한, 본 발명은 상기 제조방법으로 제조된 세사미놀 함량이 증진된 참깨 중탕 추출물을 제공한다.In addition, the present invention provides a sesame water bath extract with increased sesaminol content prepared by the above production method.
또한, 본 발명은 상기 세사미놀 함량이 증진된 참깨 중탕 추출물을 유효성분으로 함유하는 피부 미백 및 주름 개선용 화장료 조성물을 제공한다. In addition, the present invention provides a cosmetic composition for skin whitening and wrinkle improvement containing, as an active ingredient, the sesame bath extract having an increased sesaminol content.
또한, 본 발명은 상기 세사미놀 함량이 증진된 참깨 중탕 추출물을 유효성분으로 함유하는 피부 미백 및 주름 개선용 건강기능식품 조성물을 제공한다. In addition, the present invention provides a health functional food composition for skin whitening and wrinkle improvement containing the sesame bath extract with increased sesaminol content as an active ingredient.
본 발명은 세사미놀 함량이 증진된 참깨 중탕 추출물 및 상기 참깨 중탕 추출물을 유효성분으로 함유하는 피부 미백 및 주름 개선용 조성물에 관한 것으로, 참깨에 물을 첨가한 다음 pH를 2~6으로 맞춘 후, 밀폐된 용기에서 50~160℃의 온도 조건으로 10~200분 동안 중탕하는 단계;를 포함하는 본 발명의 제조방법으로 제조된 참깨 중탕 추출물은 직접적으로 열을 가해 추출한 참깨 열수 추출물에 비해 미백 및 주름 개선 효과가 현저히 증진되었다.The present invention relates to a sesame bath extract having an increased sesaminol content and a composition for skin whitening and wrinkle improvement containing the sesame bath extract as an active ingredient. After adding water to sesame seeds and adjusting the pH to 2-6, The sesame bath extract prepared by the manufacturing method of the present invention comprising the step of bathing in a water bath at a temperature of 50 to 160 ° C. in a sealed container for 10 to 200 minutes has whitening and anti-wrinkle properties compared to the sesame hot water extract extracted by direct heat. The improvement effect was markedly enhanced.
도 1은 본 발명의 참깨 중탕 추출물을 제조하는 과정을 촬영한 사진이다.
도 2는 본 발명의 참깨 중탕 추출물로부터 생성된 화합물의 분리 과정 흐름도이다.
도 3은 참깨 열수 추출물(A), 참깨 중탕 추출물(B) 및 참깨 중탕 추출물로부터 분리된 단일물질(C)의 HPLC(high-performance liquid chromatography) 크로마토그램이다.
도 4는 본 발명의 참깨 중탕 추출물의 미백 효과를 효소 유래 티로시나아제(tyrosinase) 저해 활성(A), 세포에서의 티로시나아제 저해 활성(B) 및 세포에서의 멜라닌 함량 분석(C)으로 확인한 결과이다. α-MSH(α-melanocyte-stimulating hormone)는 티로시나아제 활성 및 멜라닌 생성 유도 물질이다. 도면 내 서로 다른 문자 a~d는 서로 통계적으로 유의미한 차이가 있다는 것을 의미하며, p<0.05이다.
도 5는 본 발명의 참깨 중탕 추출물로부터 분리된 세사미놀(SHP-1)의 미백 효과를 효소 유래 티로시나아제(tyrosinase) 저해 활성(A), 세포에서의 티로시나아제 저해 활성(B) 및 세포에서의 멜라닌 함량 분석(C)으로 확인한 결과이다. α-MSH(α-melanocyte-stimulating hormone)는 티로시나아제 활성 및 멜라닌 생성 유도 물질이다. STG는 참깨의 주요 성분인 (+)-sesaminol triglucoside이고, 비타민 C 및 알부틴(Arbutin)은 양성 대조군으로 사용되었다.
도 6은 본 발명의 참깨 중탕 추출물로부터 분리된 세사미놀(SHP-1)의 미백 효과를 TRP-1(tyrosinase related protein-1), TRP-2(tyrosinase related protein-2), 티로시나아제(tyrosinase) 및 MITF(microphthalmia-associated transcription factor) 단백질 발현 변화로 확인한 결과이다. (A)는 단백질 발현 양상을 확인한 필름 사진이고, (B)는 이를 정량한 결과이다. α-MSH(α-melanocyte-stimulating hormone)는 티로시나아제 활성 및 멜라닌 생성 유도 물질이고, 알부틴(Arbutin)은 양성 대조군으로 사용되었다. 도면 내 *, **은 양성 대조군 대비 통계적으로 유의미한 차이가 있다는 것을 의미하며, *은 p<0.05, **은 p<0.01이다.
도 7은 본 발명의 참깨 중탕 추출물의 주름 개선 효과를 MMP-1(Matrix metalloproteinase-1) 함량 변화(A) 및 프로콜라겐(procollagen) 합성 정도(B)로 확인한 결과이다. UVB를 조사하여 MMP-1의 발현을 촉진하고, 프로콜라겐 합성을 저해하였다. 도면 내 서로 다른 문자 a~d는 서로 통계적으로 유의미한 차이가 있다는 것을 의미하며, p<0.05이다.
도 8은 본 발명의 참깨 중탕 추출물로부터 분리된 세사미놀의 주름 개선 효과를 콜라게나아제 저해 활성(A), MMP-1 함량 변화(B) 및 프로콜라겐 합성 정도(C)로 확인한 결과이다. UVB를 조사하여 MMP-1의 발현을 촉진하고, 프로콜라겐 합성을 저해하였다. STG는 참깨의 주요 성분인 (+)-sesaminol triglucoside이고, EGCG(Epigallocatechin gallate)는 양성 대조군으로 사용되었다.
도 9는 본 발명의 참깨 중탕 추출물로부터 분리된 세사미놀의 주름 개선 효과를 MMP-1 및 프로콜라겐 단백질 발현 변화로 확인한 결과이다. (A)는 단백질 발현 양상을 확인한 필름 사진이고, (B)는 이를 정량한 결과이다. UVB를 조사하여 MMP-1의 발현을 촉진하고, 프로콜라겐 합성을 저해하였다. STG는 참깨의 주요 성분인 (+)-sesaminol triglucoside이고, EGCG(Epigallocatechin gallate)는 양성 대조군으로 사용되었다. 도면 내 *, **은 양성 대조군 대비 통계적으로 유의미한 차이가 있다는 것을 의미하며, *은 p<0.05, **은 p<0.01이다.
도 10은 참깨 열수 추출물, 참깨 중탕 추출물 및 참깨 중탕 추출물로부터 분리한 세사미놀의 세포 독성을 B16F10 세포(A, C) 및 CCD-986sk(B, D) 세포에서 확인한 결과이다. STG는 참깨의 주요 성분인 (+)-sesaminol triglucoside이고, EGCG는 양성 대조군으로 사용된 에피갈로카테킨 갈레이트(Epigallocatechin gallate) 이다.
도 11은 SHP-1의 함량을 현저히 증가시키는 참깨 중탕 추출물의 최적 제조방법을 반응 시간(A), 참깨 비율(B), 반응 온도(C) 및 pH(D)로 나눠 확인한 결과이다. Figure 1 is a photograph taken during the process of preparing the sesame bath extract of the present invention.
Figure 2 is a flow chart of the separation process of the compound produced from the sesame water bath extract of the present invention.
3 is a high-performance liquid chromatography (HPLC) chromatogram of a single substance (C) separated from a hot-water extract of sesame (A), a bath-bath extract of sesame seeds (B), and a bath-bath of sesame seeds.
Figure 4 confirms the whitening effect of the sesame bath extract of the present invention by enzyme-derived tyrosinase inhibitory activity (A), tyrosinase inhibitory activity in cells (B), and melanin content analysis in cells (C). This is the result. α-MSH (α-melanocyte-stimulating hormone) is a substance that induces tyrosinase activity and melanin production. Different letters a to d in the figure mean that there is a statistically significant difference, p<0.05.
Figure 5 shows the whitening effect of sesaminol (SHP-1) isolated from the sesame bath extract of the present invention, enzyme-derived tyrosinase inhibitory activity (A), tyrosinase inhibitory activity in cells (B) and cell This is the result confirmed by melanin content analysis (C) in . α-MSH (α-melanocyte-stimulating hormone) is a substance that induces tyrosinase activity and melanin production. STG is (+)-sesaminol triglucoside, a major component of sesame seeds, and vitamin C and arbutin were used as positive controls.
Figure 6 shows the whitening effect of sesaminol (SHP-1) isolated from the sesame bath extract of the present invention by TRP-1 (tyrosinase related protein-1), TRP-2 (tyrosinase related protein-2), tyrosinase (tyrosinase) ) and MITF (microphthalmia-associated transcription factor) protein expression changes. (A) is a film photograph confirming the protein expression pattern, and (B) is the result of quantifying it. α-MSH (α-melanocyte-stimulating hormone) is an inducing substance for tyrosinase activity and melanin production, and arbutin was used as a positive control. *, ** in the figure means that there is a statistically significant difference compared to the positive control group, * is p <0.05, ** is p <0.01.
Figure 7 is the result of confirming the anti-wrinkle effect of the sesame bath extract of the present invention by the change in MMP-1 (Matrix metalloproteinase-1) content (A) and the degree of procollagen synthesis (B). UVB irradiation promoted the expression of MMP-1 and inhibited procollagen synthesis. Different letters a to d in the figure mean that there is a statistically significant difference, p<0.05.
8 is a result of confirming the anti-wrinkle effect of sesaminol isolated from the sesame bath extract of the present invention in terms of collagenase inhibitory activity (A), change in MMP-1 content (B), and degree of procollagen synthesis (C). UVB irradiation promoted the expression of MMP-1 and inhibited procollagen synthesis. STG is (+)-sesaminol triglucoside, a major component of sesame seeds, and EGCG (Epigallocatechin gallate) was used as a positive control.
9 shows the results of confirming the anti-wrinkle effect of sesaminol isolated from the sesame bath extract of the present invention by changes in MMP-1 and procollagen protein expression. (A) is a film photograph confirming the protein expression pattern, and (B) is the result of quantifying it. UVB irradiation promoted the expression of MMP-1 and inhibited procollagen synthesis. STG is (+)-sesaminol triglucoside, a major component of sesame seeds, and EGCG (Epigallocatechin gallate) was used as a positive control. *, ** in the figure means that there is a statistically significant difference compared to the positive control group, * is p <0.05, ** is p <0.01.
10 shows the results of confirming the cytotoxicity of sesaminol isolated from sesame hot-water extract, sesame bath-bath extract, and sesame bath-bath extract in B16F10 cells (A, C) and CCD-986sk (B, D) cells. STG is (+)-sesaminol triglucoside, a major component of sesame seeds, and EGCG is epigallocatechin gallate used as a positive control.
11 is a result of confirming the optimal method for preparing a sesame bath extract significantly increasing the content of SHP-1 by dividing the reaction time (A), sesame ratio (B), reaction temperature (C), and pH (D).
본 발명의 목적을 달성하기 위하여, 본 발명은In order to achieve the object of the present invention, the present invention
1) 세척된 참깨에 물을 첨가한 후, pH를 2~6으로 조정하는 단계;1) adding water to the washed sesame seeds and adjusting the pH to 2-6;
2) 상기 단계 1) 이후에, 50~160℃의 온도 조건으로 10~200분 동안 중탕하는 단계; 및2) after step 1), bathing in hot water for 10 to 200 minutes at a temperature of 50 to 160 ° C; and
3) 상기 단계 2) 이후에, 여과하는 단계;를 포함하는 세사미놀 함량이 증진된 참깨 중탕 추출물의 제조방법을 제공한다. 3) after step 2), filtering; provides a method for producing a sesame bath extract having increased sesaminol content.
본 발명의 일 구현 예에서, 상기 제조방법은 구체적으로 In one embodiment of the present invention, the manufacturing method is specifically
1) 세척된 참깨 10g에 대하여 0.5~2L의 물을 첨가한 후, pH를 3.5~5.5로 조정하는 단계;1) After adding 0.5 to 2 L of water to 10 g of washed sesame seeds, adjusting the pH to 3.5 to 5.5;
2) 상기 단계 1) 이후에, 밀폐된 용기 내에서 100~150℃의 온도 조건으로 60~150분 동안 증기로 중탕하는 단계; 및2) after step 1), bathing with steam for 60 to 150 minutes at a temperature of 100 to 150 ° C in a sealed container; and
3) 상기 단계 2) 이후에, 여과하는 단계;를 포함할 수 있고, 3) after step 2), filtering; may include,
더 구체적으로 more specifically
1) 세척된 참깨 10g에 대하여 0.8~1.2L의 물을 첨가한 후, pH를 3.5~5로 조정하는 단계;1) Adding 0.8 to 1.2 L of water to 10 g of washed sesame seeds and adjusting the pH to 3.5 to 5;
2) 상기 단계 1) 이후에, 밀폐된 용기 내에서 110~130℃의 온도 조건으로 110~130분 동안 증기로 중탕하는 단계; 및2) after step 1), bathing with steam for 110 to 130 minutes at a temperature of 110 to 130 ° C in a sealed container; and
3) 상기 단계 2) 이후에, 여과하는 단계;를 포함할 수 있으며, 3) after step 2), filtering; may include,
더욱더 구체적으로는 more specifically
1) 세척된 참깨 10g에 대하여 1L의 물을 첨가한 후, pH를 4~5로 조정하는 단계;1) adding 1 L of water to 10 g of washed sesame seeds and adjusting the pH to 4-5;
2) 상기 단계 1) 이후에, 밀폐된 용기 내에서 121℃의 온도 조건으로 120분 동안 증기로 중탕하는 단계; 및2) after step 1), bathing in a water bath with steam for 120 minutes at a temperature of 121° C. in an airtight container; and
3) 상기 단계 2) 이후에, 여과하는 단계;를 포함할 수 있지만, 이에 한정하는 것은 아니다. 3) after step 2), filtering; may include, but is not limited thereto.
본 발명은 또한, 상기 제조방법으로 제조된 세사미놀 함량이 증진된 참깨 중탕 추출물을 제공한다. 본 발명의 제조방법으로 제조된 참깨 중탕 추출물은 직접적으로 열을 가해 추출한 참깨 열수 추출물 대비 세사미놀 함량이 현저히 증진되는 효과가 있다. The present invention also provides a sesame water bath extract with increased sesaminol content prepared by the above production method. The sesame bath extract prepared by the manufacturing method of the present invention has the effect of significantly increasing the content of sesaminol compared to the hot-water sesame extract extracted by directly applying heat.
또한, 본 발명은 상기 세사미놀 함량이 증진된 참깨 중탕 추출물을 유효성분으로 함유하는 피부 미백 및 주름 개선용 화장료 조성물을 제공한다. In addition, the present invention provides a cosmetic composition for skin whitening and wrinkle improvement containing, as an active ingredient, the sesame bath extract having an increased sesaminol content.
본 발명의 화장료 조성물은 스킨, 스킨 소프트너, 스킨토너, 아스트린젠트, 로션, 밀크로션, 모이스쳐로션, 영양로션, 마사지크림, 영양크림, 아이 크림, 모이스쳐 크림, 핸드크림, 에센스, 영양에센스, 팩, 클렌징폼, 클렌징 워터, 클렌징 로션, 클렌징 크림, 바디로션, 바디클렌져, 비누 및 파우더로 이루어진 군으로부터 선택되는 어느 하나의 제형으로 제조될 수 있지만, 이에 한정하는 것은 아니다. 이들 각 제형의 조성물은 그 제형의 제제화에 필요하고 적절한 각종의 기제와 첨가물을 함유할 수 있으며, 이들 성분의 종류와 양은 당업자에 의해 용이하게 선정될 수 있다.The cosmetic composition of the present invention is used for skin, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, eye cream, moisture cream, hand cream, essence, nutrient essence, pack, It may be prepared in any one formulation selected from the group consisting of cleansing foam, cleansing water, cleansing lotion, cleansing cream, body lotion, body cleanser, soap and powder, but is not limited thereto. The composition of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.
본 발명의 화장료 조성물은 상기 유효성분 이외에 추가로 동일 또는 유사한 기능을 나타내는 피부 미백 또는 주름 개선용 성분을 1종 이상 함유할 수 있다. 피부 미백 피부 미백 또는 주름 개선용 성분으로는 코지산 및 이의 유도체, 알부틴, 아스코르브산 및 이의 유도체, 히드로퀴논 및 이의 유도체, 레조르시놀, 사이클로알카논, 메틸렌디옥시페닐 알칸올, 2,7-디니트로인다졸 또는 덩굴귤 추출물, 쌀 추출물, 감초 추출물과 같은 식물 추출물 등이 있으나, 이에 제한되는 것은 아니다.The cosmetic composition of the present invention may further contain one or more components for skin whitening or wrinkle improvement that exhibit the same or similar functions in addition to the above active ingredients. Skin whitening Skin whitening or wrinkle improvement components include kojic acid and its derivatives, arbutin, ascorbic acid and its derivatives, hydroquinone and its derivatives, resorcinol, cycloalkanone, methylenedioxyphenyl alkanol, 2,7-di Nitroindazole or plant extracts such as tangerine extract, rice extract, licorice extract, etc., but are not limited thereto.
본 발명의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물섬유, 식물섬유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can
본 발명의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additionally chlorofluorohydrocarbon, propellants such as propane/butane or dimethyl ether.
본 발명의 제형이 용액 또는 유탁액의 경우에는 담체 성분으로서 용매, 용매화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.
본 발명의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.
본 발명의 제형이 계면-활성제 함유 클렌징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 라놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.
본 발명의 화장료 조성물은 살진균제, 굴수성 유발물질, 보습제, 방향제, 방향제 담체, 단백질, 용해화제, 당 유도체, 일광차단제, 비타민, 식물 추출물 등을 포함하는 부형제를 추가로 함유할 수 있다.The cosmetic composition of the present invention may further contain excipients including fungicides, hydrotropes, humectants, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like.
또한, 본 발명은 상기 세사미놀 함량이 증진된 참깨 중탕 추출물을 유효성분으로 함유하는 피부 미백 및 주름 개선용 건강기능식품 조성물을 제공한다. In addition, the present invention provides a health functional food composition for skin whitening and wrinkle improvement containing the sesame bath extract with increased sesaminol content as an active ingredient.
본 발명의 피부 미백 또는 주름 개선용 건강기능식품 조성물은 분말, 과립, 환, 정제, 캡슐, 캔디, 시럽 및 음료로 이루어진 군으로부터 선택된 하나의 제형으로 제조할 수 있으나 이에 제한되지 않는다. 바람직하게는, 상기 피부 미백 또는 주름 개선용 건강기능성 식품은 이너뷰티(inner beauty) 식품 형태로 섭취함으로써 더욱 우수한 효과를 갖는 장점을 가진다. 상기 이너뷰티(inner beauty) 식품은 '먹는 화장품 또는 뷰티 식품'으로 일컬어지는 식품으로, 피부에 좋은 여러 가지 성분을 몸속으로 흡수시켜 피부 체질을 건강하게 바꾸는 식품을 지칭하며, 피부 타입에 맞는 화장품을 고르듯 피부 컨디션과 라이프스타일을 고려해 개개인에게 맞는 이너뷰티 식품을 선택하여 섭취할 수 있다. 보다 바람직하게는 상기 참깨 중탕 추출물을 포함하는 화장품과 상기 추출물을 포함하는 이너뷰티 식품을 혼용할 경우, 화장품만 사용하는 것에 비해 티로시나아제 활성 및 멜라닌 합성을 저해하고, 콜라게나아제 활성 및 MMP-1 생성을 억제하는 효과가 월등히 높아져 더욱 효과적으로 피부 미백 및 주름 개선 효과를 볼 수 있는 장점을 가진다.The health functional food composition for skin whitening or wrinkle improvement of the present invention may be prepared in one formulation selected from the group consisting of powder, granule, pill, tablet, capsule, candy, syrup and beverage, but is not limited thereto. Preferably, the health functional food for skin whitening or wrinkle improvement has the advantage of having a more excellent effect by ingesting it in the form of inner beauty food. The inner beauty food is a food referred to as 'eating cosmetics or beauty food', and refers to food that changes the skin constitution to be healthy by absorbing various ingredients that are good for the skin into the body. You can select and consume inner beauty foods that are suitable for each individual by considering skin condition and lifestyle. More preferably, when cosmetics containing the sesame bath extract and inner beauty food containing the extract are mixed, tyrosinase activity and melanin synthesis are inhibited, collagenase activity and MMP- 1 production, it has the advantage of being able to see more effective skin whitening and wrinkle improvement effects.
또한, 상기 식품의 종류에는 특별한 제한은 없다. 상기 건강기능식품 조성물을 첨가할 수 있는 식품의 예로는 육류, 소시지, 빵, 초콜릿, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차 드링크제, 알콜 음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 건강식품을 모두 포함한다. 상기 음료는 탄산음료, 기능성이온음료, 쥬스(예를 들어, 사과, 배,포도, 알로에, 감귤, 복숭아, 당근, 토마토쥬스 등), 식혜 등을 포함한다.In addition, there is no particular limitation on the type of food. Examples of foods to which the health functional food composition can be added include meat, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea There are drinks, alcoholic beverages, and vitamin complexes, and includes all health foods in a conventional sense. The beverage includes carbonated beverages, functional ionic beverages, juice (eg, apple, pear, grape, aloe, tangerine, peach, carrot, tomato juice, etc.), sikhye, and the like.
본 발명의 참깨 중탕 추출물을 식품첨가물로 사용하는 경우, 상기 추출물을 그대로 첨가하거나 다른 식품 또는 식품성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효성분은 그의 사용 목적(예방 또는 개선)에 따라 적절하게 사용될 수 있다. 일반적으로, 식품 또는 음료의 제조시에 본 발명의 추출물은 원료에 대하여 15 중량부 이하, 바람직하게는 10 중량부 이하의 양의로 첨가된다. 그러나 건강 조절을 목적으로 하는 장기간의 섭취의 경우에는 안전성 면에서 아무런 문제가 없는 범위의 양으로 사용될 수 있다.When the sesame bath extract of the present invention is used as a food additive, the extract may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. Active ingredients can be appropriately used depending on their purpose of use (prevention or improvement). In general, when preparing food or beverage, the extract of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for the purpose of health control, it can be used in an amount within a safe range.
본 발명의 기능성 식품은 식품 제조 시에 통상적으로 첨가되는 성분을 포함하며, 예를 들어, 단백질, 탄수화물, 지방, 영양소 및 조미제를 포함한다. 예컨대, 드링크제로 제조되는 경우에는 유효성분 이외에 천연 탄수화물 또는 향미제를 추가 성분으로서 포함시킬 수 있다. 상기 천연 탄수화물은 모노사카라이드(예컨대, 글루코오스, 프럭토오스 등), 디사카라이드(예컨대, 말토스, 수크로오스 등), 올리고당, 폴리사카라이드(예컨대, 덱스트린, 시클로덱스트린 등) 또는 당알코올(예컨대, 자일리톨, 소르비톨, 에리쓰리톨 등)인 것이 바람직하다. 상기 향미제는 천연 향미제(예컨대, 타우마틴, 스테비아 추출물 등)와 합성 향미제(예컨대, 사카린, 아스파르탐 등)를 이용할 수 있다. 상기 건강기능식품 조성물 외에 여러가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등을 더 함유할 수 있다.The functional food of the present invention includes components commonly added during food preparation, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink, natural carbohydrates or flavors may be included as additional ingredients in addition to active ingredients. The natural carbohydrates are monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrins, cyclodextrins, etc.) or sugar alcohols (eg, maltose, sucrose, etc.) , xylitol, sorbitol, erythritol, etc.) are preferred. As the flavoring agent, natural flavoring agents (eg, thaumatin, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used. In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonated beverages It may further contain a carbonation agent used for
상기 건강기능식품 조성물 이외에 여러 가지 영양제, 비타민, 저해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등을 더 함유할 수 있다. 이러한 상기 첨가되는 성분의 비율은 크게 중요하진 않지만 본 발명의 건강기능식품 조성물 100 중량부에 대하여, 0.01 내지 0.1 중량부의 범위에서 선택되는 것이 일반적이다.In addition to the health functional food composition, various nutrients, vitamins, inhibitors, flavoring agents, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, It may further contain a carbonating agent used in carbonated beverages. The ratio of the components to be added is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.
이하, 제조예 및 실시예를 이용하여 본 발명을 더욱 상세하게 설명하고자 한다. 이들 제조예 및 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이들에 의해 제한되지 않는다는 것은 당해 기술분야에서 통상의 지식을 가진 자에게 있어 자명한 것이다. Hereinafter, the present invention will be described in more detail using preparation examples and examples. These Preparation Examples and Examples are only for explaining the present invention in more detail, and it is obvious to those skilled in the art that the scope of the present invention is not limited thereto.
제조예 1. 참깨 중탕 추출물의 제조Preparation Example 1. Preparation of Sesame Bath Bath Extract
본 발명에 사용된 참깨(Sesamum indicum L.)는 2017년 10월 경상북도 예천군에서 수확 후 건조된 것을 사용하였다. Sesame ( Sesamum indicum L.) used in the present invention was harvested and dried in October 2017 in Yecheon-gun, Gyeongsangbuk-do.
도 1에 개시된 바와 같이 세척된 참깨 250g에 대하여 25L의 물을 첨가한 후, pH를 4~5로 조정한 다음 밀폐된 용기 내에서 121℃의 온도 조건으로 120분 동안 증기를 쐬어 중탕하였다. 이 후, 5겹의 거즈를 이용하여 여과한 다음 여액을 획득함으로써, 참깨 중탕 추출물을 제조하였다. 상기 참깨 중탕 추출물은 저온감압 농축기를 이용하여 건조한 후, 실험의 시료로 사용하였다. As shown in FIG. 1, after adding 25 L of water to 250 g of washed sesame seeds, adjusting the pH to 4-5, and then steaming in a sealed container at a temperature of 121 ° C. for 120 minutes. Thereafter, by filtering using 5 layers of gauze and obtaining a filtrate, a sesame bath bath extract was prepared. The sesame water bath extract was dried using a low-temperature vacuum concentrator, and then used as a sample for the experiment.
한편, 세척된 참깨 250g에 대하여 25L의 물을 첨가한 후 직접적으로 열을 가하여 70℃에서 90분 동안 열수 추출하여 참깨 열수 추출물을 제조하였고, 이를 실험의 비교예로 사용하였다. On the other hand, after adding 25 L of water to 250 g of washed sesame seeds, direct heat was applied to hot water extraction at 70 ° C. for 90 minutes to prepare a sesame hot water extract, which was used as a comparative example of the experiment.
실시예 1. 참깨 중탕 추출물로부터 신규 생성된 화합물의 분리 및 동정Example 1. Isolation and Identification of Newly Produced Compounds from Sesame Bath Bath Extract
1) 참깨 중탕 추출물로부터 신규 생성된 화합물의 분리1) Separation of Newly Produced Compounds from Sesame Bath Bath Extract
상기 제조예 1에서 제조한 참깨 중탕 추출물 및 참깨 열수 추출물의 성분 변화를 확인하기 위해, HPLC를 수행하였다. HPLC 분석 조건은 하기 표 1에 개시된 바와 같다. 이동상 용매 A로 0.1%(v/v) 포름산(formic acid, HCOOH)을 사용하였고, 용매 B로는 아세토니트릴(acetonitrile, MeCN)을 사용하였으며, 구배 용리(gradient elution)를 100% 용매 A로 분석을 시작하여 최종 100% 용매 B로 30분 동안, 이동상의 유속 1.0mL/min을 유지하며 280nm에서 화합물을 검출하였다.In order to confirm the change in the components of the sesame bath extract and sesame hot water extract prepared in Preparation Example 1, HPLC was performed. HPLC analysis conditions are as disclosed in Table 1 below. 0.1% (v/v) formic acid (HCOOH) was used as the mobile phase solvent A, acetonitrile (MeCN) was used as the solvent B, and gradient elution was performed with 100% solvent A. Starting with the final 100% solvent B for 30 minutes, the compound was detected at 280 nm while maintaining a flow rate of 1.0 mL/min of the mobile phase.
그 결과, 도 3A 및 3B에 개시된 바와 같이 참깨 중탕 추출물에서 참깨 열수 추출물에서는 검출되지 않았던 성분이 검출된 것을 확인하였고, 이를 도 2의 화합물 분리 과정 흐름도에 개시된 바와 같이 순차적으로 분획한 결과, 3C에 개시된 바와 같이 참깨 중탕 추출물에서 신규 생성된 화합물을 분리할 수 있었다. As a result, as shown in FIGS. 3A and 3B, it was confirmed that components that were not detected in the hot-water sesame extract were detected in the sesame bath extract, and as a result of sequential fractionation as shown in the flow chart of the compound separation process in FIG. 2, 3C As described above, the newly produced compound could be isolated from the sesame bath extract.
구체적으로, 상기 제조예 1에서 제조한 참깨 중탕 추출물 100g에 10%(v/v) MeOH를 2L 첨가하여 희석한 후, n-hexane, EtOAc 및 n-BuOH을 이용하여 순차 분획하였고, 각 분획물을 저온 감압 농축하여 건조시켜 n-hexane 분획물(871.6 mg), EtOAc 분획물(36.4g), n-BuOH(42.6g) 및 물 분획물(19.8g)을 획득하였다. Specifically, 100 g of the sesame bath extract prepared in Preparation Example 1 was diluted by adding 2 L of 10% (v/v) MeOH, and sequentially fractionated using n -hexane, EtOAc, and n -BuOH, and each fraction was After concentrating and drying at low temperature under reduced pressure, an n -hexane fraction (871.6 mg), an EtOAc fraction (36.4 g), n -BuOH (42.6 g), and a water fraction (19.8 g) were obtained.
HPLC 분석을 통해 상기 획득된 분획물 내 화합물을 확인한 결과, 참깨 중탕 추출물에서 생성된 화합물은 EtOAc 분획물에 모두 포함되어 있음을 확인하였고, EtOAc 분획물에 대하여 Toyopearl HW-40(2.5cm i.d.×42cm) 및 ODS AQ 120-50S(1.0cm, i.d.×45cm)를 충진제로 이용한 컬럼 크로마토그래피를 수행하여 70%(v/v) MeOH 용출액으로부터 SHP-1(158.2 mg, t R 21.6min)을 수득하였다. 분리된 단일물질은 NMR 및 ESIMS 스펙트럼을 측정하여 화합물의 구조를 결정하였다.As a result of confirming the compounds in the obtained fractions through HPLC analysis, it was confirmed that the compounds produced in the sesame bath bath extract were all included in the EtOAc fraction, and Toyopearl HW-40 (2.5 cm id × 42 cm) and ODS Column chromatography using AQ 120-50S (1.0 cm, id × 45 cm) as a filler was performed to obtain SHP-1 (158.2 mg, t R 21.6 min) from 70% (v / v) MeOH eluate. The separated single material was measured for NMR and ESIMS spectra to determine the structure of the compound.
2) 참깨 중탕 추출물로부터 신규 생성된 화합물의 동정2) Identification of Newly Produced Compounds from Sesame Bath Bath Extract
상기 참깨 중탕 추출물로부터 신규 생성된 화합물 SHP-1은 흰색의 무결정형 분말상 형태로 수득하였고, [α]D+736(c 0.1, MeOH)으로 측정되었다. HRESIMS 스펙트럼 측정결과 m/z 393.0943[M+Na]+의 base 피크를 확인하여 화합물의 분자량을 확인하였다. 1H NMR 스펙트럼 측정 결과 1,2,4,5-tetrasubstituted된 방향족 프로톤 시그널이 δ H 6.80(1H, s, H-6') 및 6.39(1H, s, H-3')로 측정되었고 ABX-type의 방향족 시그널이 δ H 6.89(1H, d, J=1.8Hz, H-2''), 6.86(1H, dd, J=7.8, 1.8Hz, H-6'') 및 6.79(1H, d, J=7.8Hz, H-5'')에서 측정되었다. 두 개의 methylenedioxy에 해당하는 프로톤 시그널이 δ H 5.94(2H, s, H-7'') 및 5.84(2H, s, H-7')에서 측정되었다. 또한 퍼퓨란(furfuran) 골격에 해당하는 시그널이 각 δ H 5.02(1H, d, J=4.8Hz, H-2), 4.70(1H, d, J=4.8Hz, H-6), 4.25(1H, dd, J=9.0, 7.2Hz, H-4), 4.28(1H, dd, J=9.0, 6.6Hz, H-8), 4.04(1H, dd, J=9.0, 3.6Hz, H-8), 3.88(1H, dd, J=9.0, 3.6Hz, H-4), 3.05(1H, m, H-5) 및 3.03(1H, m, H-1)에서 측정되었다. 13C NMR 및 HSQC 스펙트럼 해석에서도 세사몰(sesamol) 및 퍼퓨란(furfuran) 시그널이 관찰되었다. 2D NMR 스펙트럼 해석으로 SOP-1은 (+)-세사미놀(sesaminol)로 구조를 동정하였다.The newly produced compound SHP-1 from the sesame water bath extract was obtained in the form of a white amorphous powder, and [α] D +736 ( c 0.1, MeOH) was measured. As a result of HRESIMS spectrum measurement, the base peak of m/z 393.0943[M+Na] + was confirmed to confirm the molecular weight of the compound. As a result of 1 H NMR spectrum measurement, 1,2,4,5-tetrasubstituted aromatic proton signals were measured as δ H 6.80 (1H, s, H-6') and 6.39 (1H, s, H-3'), and ABX- The aromatic signals of type δ H 6.89 (1H, d, J =1.8Hz, H-2''), 6.86 (1H, dd, J =7.8, 1.8Hz, H-6'') and 6.79 (1H, d , J = 7.8 Hz, H-5″). Proton signals corresponding to the two methylenedioxy were measured at δ H 5.94 (2H, s, H-7'') and 5.84 (2H, s, H-7''). In addition, the signals corresponding to the furfuran backbone were δ H 5.02 (1H, d, J =4.8Hz, H-2), 4.70 (1H, d, J =4.8Hz, H-6), 4.25 (1H-6), respectively. , dd, J =9.0, 7.2Hz, H-4), 4.28 (1H, dd, J =9.0, 6.6Hz, H-8), 4.04 (1H, dd, J =9.0, 3.6Hz, H-8) , 3.88 (1H, dd, J = 9.0, 3.6 Hz, H-4), 3.05 (1H, m, H-5) and 3.03 (1H, m, H-1). Sesamol and furfuran signals were also observed in 13 C NMR and HSQC spectral analysis. 2D NMR spectrum analysis identified the structure of SOP-1 as (+)-sesaminol.
상기 결과로부터, 본 발명의 참깨 중탕 추출물의 제조방법은 참깨의 주요 성분인 (+)-sesaminol triglucoside을 가수분해하여 아글리콘(aglycone)인 (+)-세사미놀을 생성시킨다는 것을 확인하였다. From the above results, it was confirmed that the method for preparing the sesame bath extract of the present invention hydrolyzes (+)-sesaminol triglucoside, a major component of sesame, to produce (+)-sesaminol, an aglycone.
실시예 2. 참깨 중탕 추출물의 미백 효과Example 2. Whitening effect of sesame bath extract
1) 효소 유래 티로시나아제(tyrosinase) 저해 활성 평가1) Evaluation of enzyme-derived tyrosinase inhibitory activity
본 발명의 제조예 1에서 제조된 참깨 중탕 추출물과 열을 직접 가해 추출한 참깨 열수 추출물의 미백활성과 관련된 티로시아나제(tyrosinase) 저해 활성을 평가하였다.The tyrosinase inhibitory activity related to the whitening activity of the sesame bath extract prepared in Preparation Example 1 of the present invention and the sesame hot water extract extracted by direct application of heat was evaluated.
구체적으로, 67mM 인산나트륨 완충용액(sodium phosphate buffer, pH 6.8) 50㎕, 10mM L-DOPA 80㎕ 및 제조예 1의 시료 40㎕의 혼합액에 버섯 티로시나아제(mushroom tyrosinase, 110U/mL) 80㎕를 첨가한 다음 25℃에서 2분 동안 반응시켜 반응액 중에 생성된 DOPA 크롬(chrome)을 475nm에서 측정하였다. 티로시나아제(tyrosinase) 저해 활성은 하기 식 1에 개시된 바와 같이 시료 용액의 첨가구와 무첨가구의 흡광도 감소율로 나타냈다. Specifically, 80 μl of mushroom tyrosinase (110 U/mL) in a mixture of 50 μl of 67 mM sodium phosphate buffer (pH 6.8), 80 μl of 10 mM L-DOPA, and 40 μl of the sample of Preparation Example 1 was added and then reacted at 25° C. for 2 minutes, and DOPA chrome generated in the reaction solution was measured at 475 nm. As shown in
[식 1][Equation 1]
티로시나아제 저해 활성(%)=(1-시료첨가군 흡광도/무첨가구 흡광도)×100Tyrosinase inhibitory activity (%) = (1-absorbance of sample addition group/absorbance of no addition group) × 100
그 결과, 도 4A에 개시된 바와 같이 참깨 열수 추출물에 비해 본 발명의 제조방법으로 제조된 참깨 중탕 추출물의 티로시나아제 저해 활성이 현저하게 증진되는 것을 확인하였다. As a result, as shown in FIG. 4A, it was confirmed that the tyrosinase inhibitory activity of the sesame bath extract prepared by the method of the present invention was significantly enhanced compared to the hot water extract of sesame seeds.
2) 세포에서의 티로시나아제 저해 활성 및 멜라닌 함량 분석2) Analysis of tyrosinase inhibitory activity and melanin content in cells
참깨 열수 추출물 및 참깨 중탕 추출물의 미백 효과를 B16F10 멜라노마 세포를 이용하여 세포에서의 티로시나아제 저해 활성 및 멜라닌 함량을 분석함으로써 확인하였다. The whitening effect of sesame hot water extract and sesame bath extract was confirmed by analyzing tyrosinase inhibitory activity and melanin content in B16F10 melanoma cells.
구체적으로, B16F10(melanoma cell) 세포를 10%(v/v) FBS(fetal bovine serum) 및 1%(v/v) 페니실린/스트렙토마이신(penicillin/streptomycin, 100U/mL)이 첨가된 DMEM(Dulbeco's modified eagle’medium) 배지를 사용하여, 37℃, 5% CO2 배양기(incubator)에 적응시키며 계대 배양하였다. 안정화된 B16F10 세포를 12웰 플레이트에 웰당 1×105개의 세포를 접종한 후, 각 웰에 제조예 1의 시료 또는 양성 대조군을 1시간 동안 전처리한 다음 α-MSH(10nM/mL)를 처리하였고, 48시간 동안 배양하였다. 48시간 후, 1%(w/v) 트리톤 X-100(triton X-100)을 함유한 10mM 인산 완충액(phosphate buffer, pH 6.8)을 250㎕ 가하고 5분 동안 교반한 후, 세포와 용액을 모두 e-tube로 이전시키고 10,000rpm에서 5분 동안 원심분리하여 상층액을 획득한 다음, 획득한 상측액을 티로시나아제 활성 분석에 사용하고, 세포 펠렛(cell pellet)은 멜라닌 함량 분석에 사용하였다. Specifically, B16F10 (melanoma cell) cells were cultured in DMEM (Dulbeco's Using modified eagle'medium medium, 37 ℃, 5% CO 2 It was subcultured while adapting to an incubator (incubator). Stabilized B16F10 cells were inoculated with 1 × 10 5 cells per well in a 12-well plate, and each well was pretreated with the sample or positive control of Preparation Example 1 for 1 hour, and then treated with α-MSH (10 nM / mL). , and cultured for 48 hours. After 48 hours, 250 μl of 10 mM phosphate buffer (pH 6.8) containing 1% (w/v) Triton X-100 was added and stirred for 5 minutes. Transferred to an e-tube and centrifuged at 10,000 rpm for 5 minutes to obtain a supernatant, and then the obtained supernatant was used for tyrosinase activity analysis, and the cell pellet was used for melanin content analysis.
상기 원심분리 후 획득한 상층액 40㎕를 96웰 플레이트에 분주하고, 0.1M 인산나트륨 완충용액(pH 7.2)에 녹인 2mg/mL L-DOPA 200㎕를 가한 다음 37℃에서 30분 동안 배양하였다. 티로시나아제에 의해 생성된 DOPA 크롬은 475nm에서 흡광도를 측정하여 확인하였다. 40 μl of the supernatant obtained after the centrifugation was dispensed into a 96-well plate, and 200 μl of 2 mg/mL L-DOPA dissolved in 0.1 M sodium phosphate buffer (pH 7.2) was added thereto, followed by incubation at 37° C. for 30 minutes. DOPA chromium produced by tyrosinase was confirmed by measuring absorbance at 475 nm.
세포 펠렛(cell pellet)은 1N NaOH 100㎕와 증류수 200㎕를 가하고 60℃에서 1시간 동안 방치하여 멜라닌(melanin)을 완전히 녹인 후, 96웰 플레이트에 200㎕를 옮긴 후 405nm에서 흡광도를 측정하였고, 멜라닌 표준품으로 얻은 표준검량선을 이용하여 각 웰에 생성된 멜라닌 양을 산출하였다. 100 μl of 1N NaOH and 200 μl of distilled water were added to the cell pellet, left at 60° C. for 1 hour to completely dissolve melanin, and then 200 μl was transferred to a 96-well plate and absorbance was measured at 405 nm, The amount of melanin produced in each well was calculated using a standard calibration curve obtained with a melanin standard.
그 결과, 도 4B에 개시된 바와 같이 본 발명의 제조방법으로 제조된 참깨 중탕 추출물은 참깨 열수 추출물에 비해 세포의 티로시나아제 활성을 억제하는 효과가 우수하였고, 도 4C에 개시된 바와 같이 멜라닌 함량을 억제하는 효과 또한 현저히 우수하였다. As a result, as shown in FIG. 4B, the sesame bath extract prepared by the manufacturing method of the present invention was superior to the sesame hot water extract in inhibiting the tyrosinase activity of cells, and inhibited the melanin content as shown in FIG. 4C. The effect was also remarkably excellent.
실시예 3. 참깨 중탕 추출물로부터 분리된 화합물의 미백 효과Example 3. Whitening effect of compounds isolated from sesame bath bath extract
1) 효소 유래 티로시나아제(tyrosinase) 저해 활성 평가1) Evaluation of enzyme-derived tyrosinase inhibitory activity
상기 실시예 2와 동일한 방법으로 참깨 중탕 추출물로부터 분리한 화합물인 SHP-1의 효소 유래 티로시나아제 저해 활성을 평가하였다. 양성 대조군으로는 비타민 C를 사용하였고, 비교군으로 참깨의 주요 성분인 STG[(+)-sesaminol triglucoside]를 사용하였다. In the same manner as in Example 2, the enzyme-derived tyrosinase inhibitory activity of SHP-1, a compound isolated from the sesame bath extract, was evaluated. Vitamin C was used as a positive control group, and STG [(+)-sesaminol triglucoside], a major component of sesame seeds, was used as a control group.
그 결과, 도 5A에 개시된 바와 같이 참깨 중탕 추출물로부터 분리한 화합물인 SHP-1의 티로시나아제 저해 활성이 참깨 주요 성분인 STG 보다 현저히 우수한 것을 확인하였다. As a result, as shown in FIG. 5A, it was confirmed that the tyrosinase inhibitory activity of SHP-1, a compound isolated from the sesame bath extract, was significantly superior to that of STG, a major component of sesame seeds.
2) 세포에서의 티로시나아제 저해 활성 및 멜라닌 함량 분석2) Analysis of tyrosinase inhibitory activity and melanin content in cells
상기 실시예 2와 동일한 방법으로 참깨 중탕 추출물로부터 분리한 화합물인 SHP-1의 세포에서의 티로시나아제 저해 활성 및 멜라닌 함량을 분석하였다. 양성 대조군으로는 알부틴(arbutin, 100㎍/mL)을 사용하였다. Tyrosinase inhibitory activity and melanin content in cells of SHP-1, a compound isolated from sesame bath bath extract, were analyzed in the same manner as in Example 2. Arbutin (100 μg/mL) was used as a positive control.
그 결과, 도 5B에 개시된 바와 같이 참깨 중탕 추출물로부터 분리한 화합물인 SHP-1의 세포의 티로시나아제 저해 활성이 참깨 주요 성분인 STG 보다 현저히 우수하였다. As a result, as shown in FIG. 5B, the cell tyrosinase inhibitory activity of SHP-1, a compound isolated from the sesame water bath extract, was significantly superior to that of STG, a major component of sesame seeds.
또한, 도 5C에 개시된 바와 같이 참깨 중탕 추출물로부터 분리한 화합물인 SHP-1의 멜라닌 함량이 참깨 주요 성분인 STG와 비교하여 감소한 것을 확인하였다. In addition, as shown in FIG. 5C, it was confirmed that the melanin content of SHP-1, a compound isolated from the sesame bath extract, was reduced compared to STG, a major component of sesame seeds.
3) 참깨 중탕 추출물로부터 분리된 화합물의 미백 관련 단백질 발현량 측정3) Measurement of protein expression level related to whitening of compounds isolated from sesame bath extract
미백 관련 인자인 MITF, TRP1, TRP2 및 티로시나아제 단백질 발현정도를 보기 위해, B16F10 세포를 2×104 cells/mL로 희석한 후, 100mm 배양 접시(culture dish)에 분주하고, 24시간 동안 배양하여 세포를 안정화시켰다. 그 후, 배지를 제거하고, 제조예 1의 시료를 또는 양성 대조군을 1시간 동안 전처리한 다음 α-MSH(10nM/mL)를 처리하였고, 48시간 동안 배양하였다. 배양 후, 배지를 제거한 다음 PBS로 2번 세척하였고, 용해 버퍼(Lysis buffer) 100㎕를 첨가하여 용해한 다음 4℃ 12,000rpm에서 20분간 원심분리하여 상층액을 획득하였다. 상층액의 단백질량은 브래드포드 분석법(Bradford assay)으로 정량하였고, 실험 시료별 동량의 단백질을 10%의 SDS-폴리아크릴아마이드(polyacryamide)에 로딩한 후, 전기 영동하여 단백질을 크기별로 분리하였다. 분리된 단백질은 전달 장치를 이용하여 PVDF 멤브레인(membrane)으로 옮긴 다음 실온에서 1시간 동안 블로킹 버퍼[blocking buffer, 5%(w/v) skim milk]에서 반응시켰다. 반응 후, TBST를 이용하여 10분 간격으로 총 3회 세척한 다음, MITF(Santacruz, 1:1000), TRP-1(Santacruz, 1:1000), TRP-2(Santacruz, 1:1000), 티로시나아제(Santacruz, 1:1000), β-액틴(Santacruz, 1:1000) 1차 항체를 4℃에서 16시간 동안 반응시켰다. 그 후, 다시 TBST를 이용하여 10분 간격으로 총 3회 세척하고, 마우스 항-토끼 IgG HRP 또는 보바인 항-염소(bovine anti-goat) IgG HRP의 2차 항체를 1:1000으로 희석하여 실온에서 2시간 동안 반응시켰다. 반응 후, TBST를 이용하여 10분 간격으로 총 3회 세척한 다음 암실에서 ECL(Amersham Pharmacia, England) 용액으로 반응시키고, LAS4000 화학발광 검출기(LAS4000 chemiluminescence detection system, Fuji, Tokyo, Japan)를 이용하여 밴드를 확인한 후, 밀도(density)를 측정하였다.In order to examine the expression level of MITF, TRP1, TRP2 and tyrosinase, which are whitening-related factors, B16F10 cells were diluted to 2×10 4 cells/mL, then dispensed into a 100mm culture dish and cultured for 24 hours. to stabilize the cells. Thereafter, the medium was removed, and the sample of Preparation Example 1 or the positive control was pre-treated for 1 hour, then treated with α-MSH (10 nM/mL), and cultured for 48 hours. After culturing, the medium was removed, washed twice with PBS, lysed by adding 100 μl of lysis buffer, and then centrifuged at 4° C. and 12,000 rpm for 20 minutes to obtain a supernatant. The amount of protein in the supernatant was quantified by Bradford assay, and the same amount of protein for each test sample was loaded in 10% SDS-polyacrylamide, followed by electrophoresis to separate proteins by size. The separated protein was transferred to a PVDF membrane using a transfer device and reacted in a blocking buffer (5% (w/v) skim milk) for 1 hour at room temperature. After the reaction, washing with TBST three times at 10-minute intervals, MITF (Santacruz, 1:1000), TRP-1 (Santacruz, 1:1000), TRP-2 (Santacruz, 1:1000), Tiro Synase (Santacruz, 1:1000) and β-actin (Santacruz, 1:1000) primary antibodies were reacted at 4°C for 16 hours. Thereafter, it was washed again with TBST three times at 10-minute intervals, and a secondary antibody of mouse anti-rabbit IgG HRP or bovine anti-goat IgG HRP was diluted 1:1000 and incubated at room temperature. reacted for 2 hours. After the reaction, it was washed with TBST three times at 10-minute intervals, and then reacted with ECL (Amersham Pharmacia, England) solution in the dark, and using the LAS4000 chemiluminescence detection system (Fuji, Tokyo, Japan) After confirming the band, the density was measured.
그 결과, 도 6에 개시된 바와 같이 α-MSH 처리에 의해 증가된 TRP-1, TRP-2, 티로시나아제 및 MITF 단백질 발현이 SHP-1 처리에 의해 현저히 감소하는 것을 확인하였다. As a result, as shown in FIG. 6 , it was confirmed that the expression of TRP-1, TRP-2, tyrosinase, and MITF, which were increased by α-MSH treatment, was remarkably decreased by SHP-1 treatment.
실시예 4. 참깨 중탕 추출물의 주름 개선 효과Example 4. Anti-wrinkle effect of sesame bath extract
1) 참깨 중탕 추출물의 세포 내 MMP-1 저해 효과 측정1) Measurement of intracellular MMP-1 inhibitory effect of sesame bath extract
참깨 중탕 추출물의 주름 개선 효과는 CCD-986sk 세포(Human skin fibroblast cells)를 이용하여 확인하였다. The anti-wrinkle effect of sesame bath extract was confirmed using CCD-986sk cells (Human skin fibroblast cells).
구체적으로, CCD-986sk 세포는 10%(v/v) FBS(fetal bovine serum) 및 1%(v/v) 페니실린/스트렙토마이신(penicillin/streptomycin, 100U/mL)이 첨가된 DMEM(Dulbeco's modified eagle’medium) 배지를 사용하여, 37℃, 5% CO2 배양기(incubator)에 적응시키며 계대 배양하였다. 안정화된 CCD-986sk 세포를 12웰 플레이트에 각 웰당 1×105개로 분주한 다음 24시간 동안 배양한 후, 세포를 PBS로 2회 세척한 다음 무혈청 배양액을 첨가하여 24시간 동안 배양하고, 20mJ/㎠의 자외선(UVB)을 전처리한 후, 제조예 1의 시료를 다양한 농도로 첨가한 다음, CO2 배양기에서 3일 동안 배양하였다. 배양 후, 배양액을 수집하여 MMP-1 단백질 함량 측정에 사용하였다. MMP-1 ELISA 키트(R&D Systems, Minneapolis, MN, USA)의 MMP-1 표준 용액은 6.25-100ng/mL로 1/2 희석하였고, 표준 용액 100㎕와 시료 100㎕를 MMP-1 ELISA 키트의 마이크로 플레이트에 분주하고, 100㎕의 HRP 결합체(horseradish peroxidase conjugate)를 첨가한 후 2시간 동안 37℃에서 반응시켰다. 그 후, 완충용액으로 각 웰을 5회 반복 세척하여 비결합 항원을 제거한 후, 100㎕의 크로모제닉 테트라메틸 벤지다인(chromogenic tetramethyl benzidine)을 넣어 30분 동안 반응시켰다. 그 후 100㎕의 정지 시약을 넣고 15분 후에 450nm에서 ELISA 플레이트 리더기(Tecan Austria GmBH)를 이용하여 흡광도를 측정하였다.Specifically, CCD-986sk cells were prepared in DMEM (Dulbeco's modified eagle) supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin/streptomycin (100 U/mL). Using 'medium) medium, 37 ℃, 5% CO 2 It was subcultured while adapting to an incubator (incubator). Stabilized CCD-986sk cells were dispensed in 1 × 10 5 per well in a 12-well plate and cultured for 24 hours. After washing the cells twice with PBS, serum-free culture medium was added, cultured for 24 hours, and 20mJ /
그 결과, 도 7A에 개시된 바와 같이 본 발명의 제조방법으로 제조된 참깨 중탕 추출물은 자외선 조사에 의해 증가된 MMP-1의 발현을 농도의존적으로 저해하였고, 참깨 열수 추출물 대비 본 발명의 제조방법으로 제조된 참깨 중탕 추출물의 MMP-1 발현 저해 효과가 우수하였다. As a result, as shown in FIG. 7A, the sesame bath extract prepared by the manufacturing method of the present invention inhibited the increased expression of MMP-1 by UV irradiation in a concentration-dependent manner, compared to the sesame hot water extract prepared by the manufacturing method of the present invention. The sesame bath-tang extract exhibited an excellent inhibitory effect on MMP-1 expression.
3) 참깨 중탕 추출물의 세포 내 제1형 프로콜라겐 합성 측정3) Measurement of
자외선이 조사된 CCD-986sk 세포에서 본 발명의 제조방법으로 제조된 참깨 중탕 추출물의 제1형 프로폴라겐 합성능을 측정하였다. 24웰 플레이트에 각 웰당 5×104개의 세포를 접종한 후 24시간 배양하였다. 배양 후, 세포를 PBS(phosphate buffer saline)로 2회 세척한 다음 무혈청 배양액을 첨가하여 24시간 동안 배양하였다. 이후, 20mJ/㎠의 자외선(UVB)을 전처리하였고, 제조예 1의 시료를 첨가하여 48시간 동안 배양한 후, 배양액을 수집하여 제1형 프로콜라겐 합성량 측정에 사용하였다. 세포 배양액 내의 프로콜라겐 합성 정도는 제1형 프로콜라겐 C-펩타이드 어세이 키트(Takara Bio Inc., Japan)를 사용하여 제조사의 프로토콜에 따라 측정하였다. In CCD-986sk cells irradiated with ultraviolet rays, the ability to synthesize
그 결과, 도 7B에 개시된 바와 같이 자외선 조사에 의해 감소된 제1형 프로콜라겐 합성량은 본 발명의 참깨 중탕 추출물 처리에 의해 현저히 증가하였고, 이는 참깨 열수 추출물과 비교하여서도 우수한 결과임을 확인하였다. As a result, as shown in FIG. 7B, the amount of
실시예 5. 참깨 중탕 추출물로부터 분리된 화합물의 주름 개선 효과Example 5. Anti-wrinkle effect of compounds isolated from sesame bath bath extract
1) 참깨 중탕 추출물로부터 분리된 화합물의 콜라게나아제 저해 활성 평가1) Evaluation of collagenase inhibitory activity of compounds isolated from sesame water bath extract
참깨 중탕 추출물로부터 분리된 화합물의 콜라게나아제 저해 활성을 콜라게나아제 생합성량을 측정하여 평가하였다. 콜라게나아제 생합성량 측정하기 위해, 12웰 플레이트에 각 웰당 1×105개의 CCD-986sk 세포를 분주한 다음 각 웰에 실험의 시료 또는 양성 대조군(EGCG)을 첨가한 후, CO2 배양기에서 3일 동안 배양하였다. 배양 후 트립신을 처리한 다음 100mM Tris HCl(0.5mM MgCl2, 0.1% Triton X-100) 버퍼로 용해한 후, 상등액 50㎕를 96웰 플레이트에 넣고, 37℃에서 16시간 동안 반응한 후 건조기(dry oven)에서 24시간 반응시켰다. 반응 후, 증류수 200㎕를 이용하여 3번 세척하였고 0.1% 시리우스 레드 F3BA(Sirius Red F3BA)를 포함하는 포화 피코산(saturated picoric acid) 용액을 제조한 후, 각 웰에 100㎕씩 넣고, 37℃에서 1시간 동안 반응시켰다. 그 후, 10mM HCl 용액을 각 웰에 200㎕씩 넣어 5회 세척하였다. 각 웰에 0.1N NaOH 200㎕를 넣고 5분 동안 반응시킨 후, 새로운 플레이트에 옮긴 다음 540nm에서 흡광도를 측정하였다.The collagenase inhibitory activity of the compound isolated from the sesame water bath extract was evaluated by measuring the amount of collagenase biosynthesis. In order to measure the amount of collagenase biosynthesis, 1 × 10 5 CCD-986sk cells were dispensed per well in a 12-well plate, and then the experimental sample or positive control (EGCG) was added to each well, followed by 3 incubation in a CO 2 incubator. cultured for days. After incubation, trypsin was treated, dissolved in 100 mM Tris HCl (0.5 mM MgCl 2 , 0.1% Triton X-100) buffer, and 50 μl of the supernatant was put into a 96-well plate, reacted at 37 ° C for 16 hours, and then dried. oven) for 24 hours. After the reaction, it was washed three times with 200 μl of distilled water, and a saturated picoric acid solution containing 0.1% Sirius Red F3BA was prepared, and then 100 μl was added to each well and incubated at 37 ° C. reacted for 1 hour. Thereafter, 200 μl of 10 mM HCl solution was added to each well and washed 5 times. 200 μl of 0.1N NaOH was added to each well, reacted for 5 minutes, transferred to a new plate, and absorbance was measured at 540 nm.
그 결과, 도 8A에 개시된 바와 같이 참깨 중탕 추출물로부터 분리한 화합물인 SHP-1는 콜라게나아제 저해 활성이 우수한 것을 확인하였고, 참깨 주요 성분인 STG와 비교하여도 콜라게나아제 저해 활성이 현저하다는 것을 확인하였다. As a result, as shown in Figure 8A, it was confirmed that SHP-1, a compound isolated from the sesame bath extract, had excellent collagenase inhibitory activity, and compared to STG, the main component of sesame, collagenase inhibitory activity was remarkable Confirmed.
2) 참깨 중탕 추출물로부터 분리된 화합물의 세포 내 MMP-1 저해 효과 측정2) Measurement of intracellular MMP-1 inhibitory effect of compounds isolated from sesame bath bath extract
상기 실시예 4에 개시된 방법과 동일한 방법으로 참깨 중탕 추출물로부터 분리된 화합물의 세포 내 MMP-1 저해 효과를 측정하였다. In the same manner as described in Example 4, the intracellular MMP-1 inhibitory effect of the compound isolated from the sesame bath extract was measured.
그 결과, 도 8B에 개시된 바와 같이 자외선 조사에 의해 증가된 MMP-1 함량은 참깨 중탕 추출물로부터 분리한 화합물인 SHP-1 처리에 의해 감소하는 것을 확인하였고, 참깨 주요 성분인 STG는 SHP-1에 비해 MMP-1 함량 감소효과가 적었다. As a result, as shown in FIG. 8B, it was confirmed that the MMP-1 content increased by UV irradiation was reduced by treatment with SHP-1, a compound isolated from the sesame bath bath extract, and STG, a major component of sesame seeds, was found to be reduced by SHP-1 The effect of reducing the MMP-1 content was smaller than that of
3) 참깨 중탕 추출물로부터 분리된 화합물의 세포 내 제1형 프로콜라겐 합성 측정3) Measurement of
상기 실시예 4에 개시된 방법과 동일한 방법으로 참깨 중탕 추출물로부터 분리된 화합물의 세포 내 제1형 프로콜라겐 합성량을 측정하였다. In the same manner as in Example 4, the amount of intracellular synthesis of
그 결과, 도 8C에 개시된 바와 같이 자외선 조사에 의해 감소된 제1형 프로콜라겐 합성량은 참깨 중탕 추출물로부터 분리한 화합물인 SHP-1 처리에 의해 증가하였고, 이는 참깨 주요 성분인 STG의 제1형 프로콜라겐 합성능에 비해 현저하게 우수한 결과였다. As a result, as shown in FIG. 8C, the amount of synthesized
4) 참깨 중탕 추출물로부터 분리된 화합물의 주름 개선 관련 단백질 발현량 측정4) Measurement of the protein expression level related to wrinkle improvement of the compound isolated from the sesame bath bath extract
주름 개선 관련 인자인 MMP-1 및 프로콜라겐의 단백질 발현 정도를 확인하기 위해, 100mm 배양 접시에 2×104개/mL로 희석된 CCD-986sk 세포를 분주한 다음 24시간 동안 배양하였다. 그 후, 세포를 PBS로 2회 세척한 다음 무혈청 배양액을 첨가하여 24시간 동안 배양한 후, 20mJ/㎠의 자외선(UVB)을 전처리하였고, 전처리 후, 실험의 시료를 첨가한 다음, CO2 배양기에서 3일 동안 배양하였다. 배양 후, 배지를 제거한 다음 PBS로 2번 세척하였다. 그 후, 용해 버퍼(Lysis buffer) 100㎕를 첨가하여 용해한 다음 4℃ 12,000rpm에서 20분간 원심분리하여 상층액을 획득하였다. 상층액의 단백질량은 브래드포드 분석법(Bradford assay)으로 정량하였고, 실험 시료별 동량의 단백질을 10%의 SDS-폴리아크릴아마이드(polyacryamide)에 로딩한 후, 전기영동으로 단백질을 크기별로 분리하였다. 분리된 단백질은 전달 장치를 이용하여 PVDF 멤브레인(membrane)으로 옮긴 다음 실온에서 1시간 동안 블로킹 버퍼[blocking buffer, 5%(w/v) skim milk]에서 반응시켰다. 반응 후, TBST를 이용하여 10분 간격으로 총 3회 세척한 다음 MMP-1 및 프로콜라겐 1차 항체를 4℃에서 16시간 동안 반응시켰다. 그 후, 다시 TBST를 이용하여 10분 간격으로 총 3회 세척하고, 1차 항체에 대응하는 2차 항체를 희석하여 실온에서 2시간 동안 반응시켰다. 반응 후, TBST를 이용하여 10분 간격으로 총 3회 세척한 다음 암실에서 ECL(Amersham Pharmacia, England) 용액으로 반응시키고, LAS4000 화학발광 검출기(LAS4000 chemiluminescence detection system, Fuji, Tokyo, Japan)를 이용하여 밴드를 확인한 후, 밀도(density)를 측정하였다.In order to confirm the protein expression level of MMP-1 and procollagen, factors related to wrinkle improvement, CCD-986sk cells diluted at 2×10 4 cells/mL were dispensed into a 100 mm culture dish and cultured for 24 hours. Thereafter, the cells were washed twice with PBS, and then cultured for 24 hours by adding serum-free culture medium, and then pre-treated with 20 mJ/
그 결과, 도 9에 개시된 바와 같이 자외선 조사에 의해 증가된 MMP-1의 단백질 발현량은 SHP-1 처리에 의해 감소하였고, 자외선 조사에 의해 감소된 프로콜라겐 단백질 발현량은 SHP-1 처리에 의해 증가하는 것을 확인하였다. As a result, as shown in Figure 9, the protein expression level of MMP-1 increased by UV irradiation was decreased by SHP-1 treatment, and the procollagen protein expression level decreased by UV irradiation was reduced by SHP-1 treatment. confirmed to increase.
실시예 6. 참깨 중탕 추출물 및 이로부터 분리한 화합물의 세포 독성 평가Example 6. Cytotoxicity evaluation of sesame bath extract and compounds isolated therefrom
MTT 분석을 통해 세포 독성을 확인하였다. MTT 분석은 탈수소 작용에 의하여 노란색의 수용성 기질인 MTT 테트라졸리움(tetrazolium)을 청자색을 띄는 비수용성의 MTT 포르마잔[formazan, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- tetrazolium bromide]으로 환원시키는 미트콘드리아의 능력을 이용한 검사법으로, MTT 포르마잔의 농도를 540nm에서 ELISA 플레이트 리더기(Tecan Austria GmBH, Grodig, Austria)를 이용하여 측정하였다.Cytotoxicity was confirmed by MTT assay. In the MTT assay, MTT tetrazolium, a yellow water-soluble substrate, is converted into blue-purple water-insoluble MTT formazan [formazan, (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl- tetrazolium bromide], the concentration of MTT formazan was measured using an ELISA plate reader (Tecan Austria GmBH, Grodig, Austria) at 540 nm.
B16F10 또는 CCD-986sk를 96웰 플레이트에 각 웰당 5×104개가 되도록 분주하고, 시료를 농도별로 조제하여 0.02mL 첨가한 후 37℃, 5% CO2 배양기에서 24시간 동안 배양하였다. 대조군은 시료와 동량의 증류수를 첨가하여 동일한 조건으로 배양하였다. 여기에 5mg/mL 농도로 제조한 MTT 용액 0.02mL를 첨가하여 4시간 동안 배양한 후 배양액을 제거하고 각 웰당 DMSO 0.15mL를 가하여 실온에서 30분간 반응시킨 뒤 ELISA 리더기로 540nm에서 흡광도를 측정하였다. 세포 생존율 측정은 시료 용액의 첨가군와 무첨가군의 흡광도 감소율로 나타내었다.B16F10 or CCD-986sk was dispensed to 5×10 4 per well in a 96-well plate, samples were prepared by concentration, 0.02 mL was added, and incubated at 37° C., 5% CO 2 incubator for 24 hours. The control group was cultured under the same conditions by adding the same amount of distilled water as the sample. 0.02 mL of MTT solution prepared at a concentration of 5 mg/mL was added thereto and incubated for 4 hours, then the culture medium was removed, 0.15 mL of DMSO was added to each well, reacted at room temperature for 30 minutes, and absorbance was measured at 540 nm with an ELISA reader. The cell viability was measured by the decrease in absorbance of the sample solution added group and non-added group.
그 결과, 도 10에 개시된 바와 같이 본 발명의 참깨 중탕 추출물, 참깨 열수 추출물, STG, SHP-1 및 EGCG는 미백 및 주름 개선 효과를 나타내는 농도에서 세포 독성이 없는 안전한 물질임을 확인하였다. As a result, as shown in FIG. 10, it was confirmed that the sesame bath extract, sesame hot water extract, STG, SHP-1, and EGCG of the present invention are safe substances without cytotoxicity at concentrations exhibiting whitening and wrinkle-improving effects.
실시예 7. 세사미놀 함량이 증진된 참깨 중탕 추출물 제조방법 최적 조건 확립Example 7. Establishment of Optimal Conditions for Manufacturing Method of Sesame Bath Extract with Increased Sesaminol Content
상기 실시예를 통해 본 발명의 제조방법으로 제조된 참깨 중탕 추출물의 참깨 열수 추출물 대비 증가된 미백 및 주름 개선 효과는 중탕 추출과정에서 생성된 SHP-1에 의한 것으로 판단되어, SHP-1의 함량을 현저히 증가시키는 최적 제조방법을 확인하고자 하였다. Through the above examples, the increased whitening and wrinkle improvement effects of the sesame bath extract prepared by the manufacturing method of the present invention compared to the sesame hot water extract were determined to be due to SHP-1 generated in the bath bath extraction process, and the content of SHP-1 It was intended to identify the optimal manufacturing method that significantly increases.
최적 조건의 확인을 위해, 도 11에 개시된 바와 같이 반응 시간, 참깨 중탕 비율, 반응 온도 및 pH를 조절하여 SHP-1의 함량을 확인하였다. In order to confirm the optimal conditions, the content of SHP-1 was confirmed by adjusting the reaction time, sesame water bath ratio, reaction temperature, and pH as shown in FIG. 11 .
그 결과, 참깨 1g에 대하여 100mL의 물을 첨가한 후, pH를 4~5로 조정한 다음 밀폐된 용기 내에서 121℃의 온도 조건으로 120분 동안 증기로 중탕한 경우, SHP-1의 함량이 가장 증가하는 것을 확인하였다. As a result, after adding 100 mL of water to 1 g of sesame seeds, adjusting the pH to 4 to 5, and then bathing with steam for 120 minutes at a temperature of 121 ° C in a sealed container, the content of SHP-1 found to increase the most.
Claims (7)
2) 상기 단계 1) 이후에, 50~160℃의 온도 조건으로 10~200분 동안 중탕하는 단계; 및
3) 상기 단계 2) 이후에, 여과하는 단계;를 포함하는 세사미놀 함량이 증진된 참깨 중탕 추출물의 제조방법.1) adding water to the washed sesame seeds and adjusting the pH to 2-6;
2) after step 1), bathing in hot water for 10 to 200 minutes at a temperature of 50 to 160 ° C; and
3) After step 2), filtering; A method for producing a sesame bath extract with increased sesaminol content.
1) 세척된 참깨 10g에 대하여 0.5~2L의 물을 첨가한 후, pH를 3.5~5.5로 조정하는 단계;
2) 상기 단계 1) 이후에, 밀폐된 용기 내에서 100~150℃의 온도 조건으로 60~150분 동안 증기로 중탕하는 단계; 및
3) 상기 단계 2) 이후에, 여과하는 단계;를 포함하는 것을 특징으로 하는 세사미놀 함량이 증진된 참깨 중탕 추출물의 제조방법.According to claim 1,
1) After adding 0.5 to 2 L of water to 10 g of washed sesame seeds, adjusting the pH to 3.5 to 5.5;
2) after step 1), bathing with steam for 60 to 150 minutes at a temperature of 100 to 150 ° C in a sealed container; and
3) After step 2), filtering; method for producing a sesame bath extract with increased sesaminol content, characterized in that it comprises.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020210191388A KR102661161B1 (en) | 2021-12-29 | 2021-12-29 | Sesame bath extract with increased sesaminol content and use thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020210191388A KR102661161B1 (en) | 2021-12-29 | 2021-12-29 | Sesame bath extract with increased sesaminol content and use thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20230101354A true KR20230101354A (en) | 2023-07-06 |
KR102661161B1 KR102661161B1 (en) | 2024-04-26 |
Family
ID=87185539
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020210191388A KR102661161B1 (en) | 2021-12-29 | 2021-12-29 | Sesame bath extract with increased sesaminol content and use thereof |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR102661161B1 (en) |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07145066A (en) * | 1993-11-22 | 1995-06-06 | Nisshin Oil Mills Ltd:The | Production of lignan glycoside and lignans |
US20050175723A1 (en) * | 2002-07-23 | 2005-08-11 | Fuji Oil Company Limited | Antioxidant and process for producing the same |
US20070166255A1 (en) * | 2004-11-22 | 2007-07-19 | Gupta Shyam K | Treatment of Topical Discomforts Including Acne, Sunburn, Diaper Rash, Wound, Wrinkles and Dandruff/Hair Loss by Natural Lignans via Fatty Acid Desaturase Inhibition |
JP2014097932A (en) * | 2012-11-13 | 2014-05-29 | Kadoya Sesami Mills Inc | Hydrothermal treatment extract derived from sesame, producing method thereof and product using it |
JP2015013828A (en) * | 2013-07-04 | 2015-01-22 | 公立大学法人大阪市立大学 | Composition for promoting collagen production, for promoting elastin production, and/or for promoting keratinocyte migration |
KR20160053172A (en) * | 2014-10-31 | 2016-05-13 | 주식회사 바이오랜드 | Composition for skin-whitening comprising extract of Boehmeria nivea (L.) GAUDICH Root |
KR101766656B1 (en) * | 2016-10-26 | 2017-08-09 | 백승훈 | manufacturing method of sesame oil and perilla oil for decreasing benzopyrene |
KR102017132B1 (en) * | 2018-10-11 | 2019-09-03 | 임승호 | Cosmetic composition comprising a mixed fermentation extract of cinnamomum verum and angelica dahurica |
-
2021
- 2021-12-29 KR KR1020210191388A patent/KR102661161B1/en active IP Right Grant
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07145066A (en) * | 1993-11-22 | 1995-06-06 | Nisshin Oil Mills Ltd:The | Production of lignan glycoside and lignans |
US20050175723A1 (en) * | 2002-07-23 | 2005-08-11 | Fuji Oil Company Limited | Antioxidant and process for producing the same |
US20070166255A1 (en) * | 2004-11-22 | 2007-07-19 | Gupta Shyam K | Treatment of Topical Discomforts Including Acne, Sunburn, Diaper Rash, Wound, Wrinkles and Dandruff/Hair Loss by Natural Lignans via Fatty Acid Desaturase Inhibition |
JP2014097932A (en) * | 2012-11-13 | 2014-05-29 | Kadoya Sesami Mills Inc | Hydrothermal treatment extract derived from sesame, producing method thereof and product using it |
JP2015013828A (en) * | 2013-07-04 | 2015-01-22 | 公立大学法人大阪市立大学 | Composition for promoting collagen production, for promoting elastin production, and/or for promoting keratinocyte migration |
KR20160053172A (en) * | 2014-10-31 | 2016-05-13 | 주식회사 바이오랜드 | Composition for skin-whitening comprising extract of Boehmeria nivea (L.) GAUDICH Root |
KR101766656B1 (en) * | 2016-10-26 | 2017-08-09 | 백승훈 | manufacturing method of sesame oil and perilla oil for decreasing benzopyrene |
KR102017132B1 (en) * | 2018-10-11 | 2019-09-03 | 임승호 | Cosmetic composition comprising a mixed fermentation extract of cinnamomum verum and angelica dahurica |
Also Published As
Publication number | Publication date |
---|---|
KR102661161B1 (en) | 2024-04-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US8501246B2 (en) | Cosmetic composition containing lingonberry extract and its uses | |
KR101995807B1 (en) | Method for Preparing Zizania latifolia Extract Containing Increased Content of Tricin Treated with Enzymes, and Composition for Whitening, Improving Wrinkle, Anti-inflammatory, Anti-allergy and Moisturizing Made thereby | |
KR20200048158A (en) | Composition for antioxidant or improving skin wrinkle containing defatted Perilla frutescens extract | |
KR102153355B1 (en) | A composition comprising Caulerpa racemosa extracts or fraction having anti-oxidation or anti-inflammation activity | |
KR20110054747A (en) | A composition for skin wrinkle improvement comprising extracts or fractions of eremochloa ophiuroides as an active ingredient | |
KR20110040363A (en) | A composition for skin whitening comprising extracts or fractions of eremochloa ophiuroides as an active ingredient | |
KR102000550B1 (en) | Composition comprising Polyamine compounds isolated from Quercus Mongolica pollen extracts for whitening | |
KR102661161B1 (en) | Sesame bath extract with increased sesaminol content and use thereof | |
KR102683158B1 (en) | Manufacturing method of high-speed fermented sesame with enhanced content of episesaminone diglucoside and sesaminol diglucoside | |
KR102017377B1 (en) | Cosmetic composition for skin improvement Containing Orostachys japonicus extract | |
KR102539619B1 (en) | Composition for improving skin conditions comprising Praeruptorin A | |
KR20230033388A (en) | Compositions for Improving Skin Wrinkles and Skin Whitening Using an Extract of Veratrum versicolor | |
KR102238695B1 (en) | Skin whitening composition comprising gamma-ray irradiated kaki-tannin oligomer as effective component | |
KR20210047588A (en) | Skin-lightening Composition Using an Extract of Xanthium canadense | |
KR102503190B1 (en) | Composition for improving skin conditions comprising Ajugol | |
KR102670660B1 (en) | Manufacturing method of methyl gallate oxidation reactant containing hexahydroxydiphenic acid dimethyl ester | |
KR20230158140A (en) | Method for preparing plasma-treated reactant of ergosterol, reactant prepared by the method, compound isolated therefrom, and uses thereof | |
KR102498762B1 (en) | Composition for anti-wrinkle effect comprising fermented product of Momordica charantia or compound isolated from therefrom as effective component | |
KR102264006B1 (en) | Composition for inhibiting production of melanin and promoting decomposition of melanin | |
KR102539089B1 (en) | Composition for skin whitening and anti-oxidant comprising fermented product of Angelica dahurica extract as effective component | |
KR102438389B1 (en) | Composition for preventing or improving skin wrinkle comprising stilbene derivative from Vitis vinifera root as effective component | |
KR102034779B1 (en) | Cosmetic composition for skin whitening comprising ginsenoside Rh1 and Rg2 and extract of Hydrangea macrophylla | |
KR20120041965A (en) | Compositions for preventing or treating skin damage by ultraviolet rays | |
KR20210106661A (en) | Composition for improving skin whitening and anti-wrinkle comprising Hizikia fusiformis extract as effective component | |
KR102014646B1 (en) | Compound from Caragana sinica and composition for skin whitening comprising the same |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant |