KR20220114490A - Pharmaceutical composition for prevention or treatment of intestinal damage comprising Leuconostoc citreum as active ingredient - Google Patents
Pharmaceutical composition for prevention or treatment of intestinal damage comprising Leuconostoc citreum as active ingredient Download PDFInfo
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- KR20220114490A KR20220114490A KR1020220014825A KR20220014825A KR20220114490A KR 20220114490 A KR20220114490 A KR 20220114490A KR 1020220014825 A KR1020220014825 A KR 1020220014825A KR 20220014825 A KR20220014825 A KR 20220014825A KR 20220114490 A KR20220114490 A KR 20220114490A
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- intestinal damage
- composition
- preventing
- strain
- active ingredient
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/31—Leuconostoc
- A23V2400/315—Citreum
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Abstract
Description
본 발명은 류코노스톡 시트래움(Leuconostoc citreum) 균주 또는 이의 배양물을 유효성분으로 포함하는 장 손상(intestinal damage), 구체적으로는 염증성 장질환, 비알콜성 지방간염, 항암 치료로 인한 장 손상에 대한 예방, 개선 또는 치료용 조성물에 관한 것이다. The present invention relates to intestinal damage containing a Leuconostoc citreum strain or a culture thereof as an active ingredient, specifically inflammatory bowel disease, non-alcoholic steatohepatitis, and intestinal damage caused by anticancer treatment. It relates to a composition for prevention, improvement or treatment of
현대인의 육류 위주의 식생활, 인스턴트 및 가공식품위주의 식습관, 자극성 있는 식품의 지나친 섭취, 바쁜 생활환경으로 성급해진 음식물 섭취 패턴의 변화 등 바람직하지 못한 식생활과 건전하지 못한 생활습관 그리고 나이가 들어감에 따라서 유익한 균들보다 유해한 균들이 우세하게 되어 장내 세균의 균형이 깨어져 장의 건강에 많은 악영향을 미치게 된다.Modern people's meat-centered diet, instant and processed food-oriented eating habits, excessive consumption of stimulant foods, changes in food intake patterns hastily due to busy living environment, etc. Harmful bacteria prevail over beneficial bacteria, and the balance of intestinal bacteria is disrupted, which adversely affects intestinal health.
현대인은 다양한 장 질환에 노출되어 있으며 많은 사람들이 염증성 장질환, 비알콜성 지방간염, 대장암 등의 질병으로 인해 장이 손상될 뿐 아니라 치료 후에도 장 손상으로 인해 장의 손상 회복 및 기능 유지 등이 문제가 되고 있다.Modern people are exposed to various intestinal diseases, and many people not only suffer from intestinal damage due to diseases such as inflammatory bowel disease, nonalcoholic steatohepatitis, and colorectal cancer, but also have problems recovering intestinal damage and maintaining function after treatment. is becoming
장균총(microbiota)은 미생물 균총으로 환경 내 미생물 군집을 말하며, 장균총은 숙주 예를 들어, 사람의 면역, 대사물질 등의 항상성 유지에 중요한 역할을 한다고 알려져 있다. 장균총과 숙주는 화학물질 신호를 주고받으며 장균총에 의한 면역세포의 발현이나 신경전달물질 생성, 단쇄지방산(SCFA; Short chain fatty acids) 등이 숙주 내 체계에 비대한 영향을 미치며, 특히 프로바이오틱스/프리바이오틱스는 숙주의 불균형한 장균총을 균형화하고, 이에 따른 건강한 장균총의 대사산물이 호스트의 건강을 증진시킬 수 있다.The intestinal flora (microbiota) refers to the microbial community in the environment as a microbial flora, and it is known that the intestinal flora plays an important role in maintaining the homeostasis of a host, for example, human immunity and metabolites. The intestinal flora and the host exchange chemical signals, and the expression of immune cells, the production of neurotransmitters, and short chain fatty acids (SCFA) by the intestinal flora have a bloated effect on the host system, especially probiotics/ Prebiotics balance the unbalanced intestinal flora of the host, and thus metabolites of the healthy intestinal flora can improve the health of the host.
현재, 프로바이오틱스를 유효성분으로 포함하는 알코올성 장 손상 예방 또는 치료용 조성물(한국등록특허 제10-2038695호) 내지는 신규한 락토바실러스 아시도필루스 균주를 포함하는 장내 환경 개선용 조성물(한국등록특허 제10-2201517호)이 공개되어 있으나 류코노스톡 시트래움 균주를 유효성분으로 하는 장 손상의 예방 또는 치료 효과에 대해서는 기재된 바 없다.Currently, a composition for preventing or treating alcoholic intestinal damage containing probiotics as an active ingredient (Korean Patent No. 10-2038695) or a composition for improving the intestinal environment including a novel Lactobacillus acidophilus strain (Korean Patent No. No. 10-2201517) has been published, but there is no description about the effect of preventing or treating intestinal damage using the Leukonostok Citraum strain as an active ingredient.
이러한 배경 하에서 본 발명자들은 독성 및 부작용이 거의 없는 류코노스톡 시트래움 균주 또는 이의 배양물을 포함하는 조성물이 염증성 장질환, 비알콜성 지방간염, 항암 치료 등으로 인해 손상된 장에 대해 장 손상 치료 및 기능 회복 효과가 있음을 확인하여 본 발명을 완성하였다Under this background, the present inventors have found that a composition comprising a leuconostoc citraum strain or a culture thereof having little toxicity and side effects is a treatment for intestinal damage and The present invention was completed by confirming that there is a functional recovery effect
본 발명의 목적은 류코노스톡 시트래움 균주를 유효성분으로 포함하는 장 손상의 예방 또는 치료용 약학 조성물을 제공하기 위한 것이다.It is an object of the present invention to provide a pharmaceutical composition for preventing or treating intestinal damage comprising a Leukonostok citraum strain as an active ingredient.
또한, 본 발명의 다른 목적은 류코노스톡 시트래움 균주를 유효성분으로 포함하는 장 손상의 예방 또는 개선용 식품 조성물 또는 식품 첨가제 조성물을 제공하기 위한 것이다.In addition, another object of the present invention is to provide a food composition or food additive composition for preventing or improving intestinal damage comprising a Leukonostok citraum strain as an active ingredient.
또한, 본 발명의 또 다른 목적은 류코노스톡 시트래움 균주를 유효성분으로 포함하는 가축의 장 손상의 예방 또는 개선용 사료 조성물 또는 사료 첨가제 조성물을 제공하기 위한 것이다.In addition, another object of the present invention is to provide a feed composition or feed additive composition for preventing or improving intestinal damage of livestock comprising a Leukonostok citraum strain as an active ingredient.
상기 목적을 달성하기 위하여, 류코노스톡 시트래움 균주 또는 이의 배양물을 유효성분으로 포함하는 장 손상의 예방 또는 치료용 약학 조성물을 제공한다.In order to achieve the above object, there is provided a pharmaceutical composition for preventing or treating intestinal damage comprising a Leukonostok citraum strain or a culture thereof as an active ingredient.
또한, 본 발명은 류코노스톡 시트래움 균주 또는 이의 배양물을 유효성분으로 포함하는 장 손상의 예방 또는 개선용 식품 조성물 또는 식품 첨가제 조성물을 제공한다.In addition, the present invention provides a food composition or food additive composition for preventing or improving intestinal damage comprising a Leukonostok citraum strain or a culture thereof as an active ingredient.
또한, 본 발명은 류코노스톡 시트래움 균주 또는 이의 배양물을 유효성분으로 포함하는 가축의 장 손상의 예방 또는 개선용 사료 조성물 또는 사료 첨가제 조성물을 제공한다.In addition, the present invention provides a feed composition or feed additive composition for preventing or improving intestinal damage of livestock comprising a Leukonostok citraum strain or a culture thereof as an active ingredient.
상기 목적을 달성하기 위하여, 류코노스톡 시트래움 균주 또는 이의 배양물을 유효성분으로 포함하는 장 손상의 예방 또는 치료용 약학 조성물을 제공한다.In order to achieve the above object, there is provided a pharmaceutical composition for preventing or treating intestinal damage comprising a Leukonostok citraum strain or a culture thereof as an active ingredient.
또한, 본 발명은 류코노스톡 시트래움 균주 또는 이의 배양물을 유효성분으로 포함하는 장 손상의 예방 또는 개선용 식품 조성물 또는 식품 첨가제 조성물을 제공한다.In addition, the present invention provides a food composition or food additive composition for preventing or improving intestinal damage comprising a Leukonostok citraum strain or a culture thereof as an active ingredient.
또한, 본 발명은 류코노스톡 시트래움 균주 또는 이의 배양물을 유효성분으로 포함하는 가축의 장 손상의 예방 또는 개선용 사료 조성물 또는 사료 첨가제 조성물을 제공한다.In addition, the present invention provides a feed composition or feed additive composition for preventing or improving intestinal damage of livestock comprising a Leukonostok citraum strain or a culture thereof as an active ingredient.
도 1은 Caco2 장세포에 본 발명에 따른 조성물을 처리 후 ZO1 단백질의 유전자 발현량을 측정한 그래프를 나타내는 도이다.
도 2는 CDHFD 마우스 모델에서 본 발명에 따른 조성물을 처리 후 Occludin 단백질의 유전자 발현량을 측정한 그래프를 나타내는 도이다.
도 3은 CDHFD 마우스 모델에서 본 발명에 따른 조성물을 처리 후 ZO1 단백질의 유전자 발현량을 측정한 그래프를 나타내는 도이다.1 is a diagram showing a graph measuring the gene expression level of ZO1 protein after treating Caco2 enterocytes with a composition according to the present invention.
2 is a diagram showing a graph measuring the gene expression level of Occludin protein after treatment with the composition according to the present invention in a CDHFD mouse model.
3 is a diagram showing a graph measuring the gene expression level of ZO1 protein after treatment with the composition according to the present invention in a CDHFD mouse model.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .
본 발명의 류코노스톡 시트래움 균주 또는 이의 배양물을 유효성분으로 포함하는 조성물은 장 손상의 예방 또는 치료 효과를 가지며, 약제학적 조성물로 이용될 수 있다.The composition comprising the Leukonostok citraum strain of the present invention or a culture thereof as an active ingredient has a preventive or therapeutic effect on intestinal damage, and can be used as a pharmaceutical composition.
본 발명의 류코노스톡 시트래움 균주는 유산균 균주이다. 상기 류코노스톡 시트래움 균주는 프로바이오틱스로서, 유산균의 일반적인 정장 효과 및 면역 증강 효과를 갖는다. 류코노스톡 속의 유산균이 정장 효과 면역 증강 효과를 갖는 것은 잘 알려진 사실이다.Leukonostok citraum strain of the present invention is a lactic acid bacteria strain. The leuconostok citraum strain is a probiotic, and has a general intestinal effect and immune enhancing effect of lactic acid bacteria. It is a well-known fact that lactic acid bacteria of the genus Leukonostok have an effect on the intestines and immunity enhancement.
상기 류코노스톡 시트래움 균주는 류코노스톡 시트래움 WiKim0104 균주일 수 있으며, 서열번호 1의(SEQ ID NO: 1)의 핵산서열을 가질 수 있으나, 이에 한정되지 않는다.The leucono stock citraum strain may be a leukono stock citraum WiKim0104 strain, and may have a nucleic acid sequence of SEQ ID NO: 1 (SEQ ID NO: 1), but is not limited thereto.
상기 류코노스톡 시트래움 균주는 MRS 액체배지에 0.1 내지 10% 접종하여 25 내지 37℃에서 4시간 내지 48시간 동안 배양하여 이용할 수 있다. The Leukonostok citraum strain can be used by inoculating 0.1 to 10% in MRS liquid medium and culturing at 25 to 37° C. for 4 to 48 hours.
상기 배양의 방법은 정치배양 방법이 바람직하나, 이에 한정되지 않는다. The culture method is preferably a stationary culture method, but is not limited thereto.
본 발명에 있어서, '프로바이오틱스(probiotics)'는 사람을 포함한 동물의 위장관 내에서 숙주의 장내 미생물 환경을 개선하여 숙주의 건강에 유익한 영향을 주는 살아있는 미생물'이라는 의미로 이해된다. 프로바이오틱스는 프로바이오틱 활성을 갖는 살아있는 미생물로 단일 또는 복합균주 형태로 사람이나 동물에 건조된 세포 형태나 발효산물 형태로 급여될 경우, 숙주의 장내 균총에 유익한 영향을 미칠 수 있다.In the present invention, 'probiotics' is understood to mean 'living microorganisms that have a beneficial effect on the health of the host by improving the intestinal microbial environment of the host in the gastrointestinal tract of animals including humans'. Probiotics are living microorganisms with probiotic activity, and when fed to humans or animals in the form of single or complex strains, in the form of dried cells or fermentation products, it can have a beneficial effect on the intestinal flora of the host.
상기 장 손상은 염증성 장질환, 비알콜성 지방간염 또는 항암 치료에 의한 장 손상일 수 있으며, 이에 한정되지 않는다.The intestinal damage may be inflammatory bowel disease, non-alcoholic steatohepatitis, or intestinal damage caused by chemotherapy, but is not limited thereto.
본 발명에 따른 조성물에 포함되는 류코노스톡 시트래움 균주는 생균체 또는 사균체로서 존재할 수 있으며, 또한 건조 또는 동결건조된 형태로 존재할 수도 있다. 또한, 류코노스톡 시트래움 균주의 배양물이 유효성분일 수 있으며, 상기 배양물에는 생균 배양액 또는 사균 상등액이 포함될 수 있다. 다양한 조성물 내에 포함시키기 적합한 유산균의 형태 및 제제화 방법은 당업자에게 잘 알려져 있다.The Leukonostok citraum strain included in the composition according to the present invention may exist as a live cell or a dead cell, and may also exist in a dried or lyophilized form. In addition, the culture of the Leukonostok citraum strain may be an active ingredient, and the culture may include a live cell culture medium or a dead cell supernatant. Forms of lactic acid bacteria suitable for inclusion in various compositions and methods of formulation are well known to those skilled in the art.
상기 조성물은 경구 또는 비경구로 투여할 수 있다. 비경구 투여인 경우에는 정맥 내 주입, 피하 주입, 근육 주입, 복강 주입, 내피 투여, 국소 투여, 비내 투여, 폐 내 투여 및 직장 내 투여 등으로 투여할 수 있으며, 바람직하게는 정맥 내 주입방법으로 투여할 수 있으나, 이에 한정되지 않는다.The composition may be administered orally or parenterally. In the case of parenteral administration, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, endothelial administration, topical administration, intranasal administration, intrapulmonary administration and rectal administration, etc. may be administered, preferably by intravenous injection. can be administered, but is not limited thereto.
상기 조성물의 적합한 투여량은 제제화 방법, 투여 방식, 환자의 연령, 체중, 성, 병적 상태, 음식, 투여시간, 투여경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하게 처방될 수 있다.A suitable dosage of the composition may be variously prescribed according to factors such as formulation method, administration method, age, weight, sex, pathological condition, food, administration time, administration route, excretion rate, and response sensitivity of the patient.
본 발명의 조성물이 약제학적 조성물로 활용될 경우, 본 발명의 약제학적 조성물은 상기 유효성분 이외에 약제학적으로 적합하고 생리학적으로 허용되는 보조제를 사용하여 제조될 수 있으며, 상기 보조제로는 부형제, 붕해제, 감미제, 결합제, 피복제, 팽창제, 윤활제, 활택제 또는 향미제 등을 사용할 수 있다.When the composition of the present invention is utilized as a pharmaceutical composition, the pharmaceutical composition of the present invention may be prepared using a pharmaceutically suitable and physiologically acceptable adjuvant in addition to the active ingredient, and the adjuvant includes an excipient, bog Release, sweetener, binder, coating agent, swelling agent, lubricant, lubricant or flavoring agent and the like can be used.
상기 약제학적 조성물은 투여를 위해서 상기 기재한 유효성분 이외에 추가로 약제학적으로 허용가능한 담체를 1종 이상 포함하여 약제학적 조성물로 바람직하게 제제화할 수 있다.The pharmaceutical composition may be preferably formulated as a pharmaceutical composition by including one or more pharmaceutically acceptable carriers in addition to the active ingredients described above for administration.
예를 들어, 정제 또는 캡슐제의 형태로의 제제화를 위해, 유효성분은 에탄올, 글리세롤, 물 등과 같은 경구, 무독성의 약제학적으로 허용가능한 불활성 담체와 결합될 수 있다. 또한, 원하거나 필요한 경우, 적합한 결합제, 윤활제, 붕해제 및 발색제 또한 혼합물로 포함될 수 있다. 적합한 결합제는 이에 제한되는 것은 아니나, 녹말, 젤라틴, 글루코스 또는 베타-락토오스와 같은 천연당, 옥수수감미제, 아카시아, 트래커캔스 또는 소듐올레이트와 같은 천연 및 합성검, 소듐 스테아레이트, 마그네슘 스테아레이트, 소듐 벤조에이트, 소듐아세테이트, 소듐클로라이드 등을 포함한다. 붕해제는 이에 제한되는 것은 아니나, 녹말, 메틸셀룰로스, 아가, 벤토니트, 잔탄검 등을 포함한다. 액상 용액으로 제제화되는 조성물에 있어서 허용가능한 약제학적 담체로는, 멸균 및 생체에 적합한 것으로서, 식염수, 멸균수, 링거액, 완충식염수, 알부민 주사 용액, 덱스트로즈 용액, 말토덱스트린 용액, 글리세롤, 에탄올 및 이들 성분 중 1 성분이상을 혼합하여 사용할 수 있으며, 필요에 따라 항산화제, 완충액, 정균제 등 다른 통상의 첨가제를 첨가할 수 있다. 또한 희석제, 분산제, 계면활성제, 결합제 및 윤활제를 부가적으로 첨가하여 수용액, 현탁액, 유탁액 등과 같은 주사용 제형, 환약, 캡슐, 과립 또는 정제로 제제화할 수 있다.For example, for formulation in the form of a tablet or capsule, the active ingredient may be combined with an orally, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or required, suitable binders, lubricants, disintegrants and color-developing agents may also be included in the mixture. Suitable binders include, but are not limited to, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tracacanth or sodium oleate, sodium stearate, magnesium stearate, sodium benzoate. ate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methylcellulose, agar, bentonite, xanthan gum, and the like. In the composition formulated as a liquid solution, acceptable pharmaceutical carriers, which are sterile and biocompatible, include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostats may be added as needed. In addition, diluents, dispersants, surfactants, binders and lubricants may be additionally added to form an injectable formulation such as an aqueous solution, suspension, emulsion, etc., pills, capsules, granules or tablets.
나아가 해당 분야의 적절한 방법으로 Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA에 개시되어 있는 방법을 이용하여 각 질환에 따라 또는 성분에 따라 바람직하게 제제화할 수 있다.Furthermore, it can be preferably formulated according to each disease or component using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA by an appropriate method in the field.
본 발명의 류코노스톡 시트래움 균주 또는 이의 배양물을 유효성분으로 포함하는 조성물은 장 손상의 예방 또는 개선용 식품 조성물 또는 식품 첨가제 조성물로 이용될 수 있다.The composition comprising the leuconostok citraum strain of the present invention or a culture thereof as an active ingredient may be used as a food composition or a food additive composition for preventing or improving intestinal damage.
상기 식품 조성물은 건강기능식품의 형태일 수 있다.The food composition may be in the form of a health functional food.
상기 "건강기능식품"은 건강기능식품에 관한 법률에 따라 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 제조 및 가공한 식품을 의미(제3조 제1호)하며, "기능성"이라 함은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건 용도에 유용한 효과를 얻는 것(동조 제2호)을 의미한다.The above "health functional food" means food manufactured and processed using raw materials or ingredients useful for the human body in accordance with the Health Functional Food Act (Article 3, No. 1), and "functionality" means It refers to obtaining useful effects for health purposes such as regulating nutrients or physiological effects on the structure and function of the human body (Article 2).
상기 식품 조성물은 식품 첨가물을 추가로 포함할 수 있으며, "식품첨가물"로서의 적합여부는 다른 규정이 없는 한 식품의약품안전처에 승인된 식품첨가물공전의 총칙 및 일반시험법 등에 따라 해당 품목에 관한 규격 및 기준에 의하여 판정한다.The food composition may additionally contain food additives, and the suitability as a "food additive" is determined according to the general rules and general test methods of the Food Additives Code approved by the Ministry of Food and Drug Safety, unless otherwise specified. and criteria.
상기 "식품첨가물공전"에 수재된 품목으로 예를 들어, 케톤류, 글리신, 구연산칼륨, 니코틴산, 계피산 등의 화학적 합성품, 감색소, 감초추출물, 결정셀롤로오스, 구아검 등의 천연첨가물, L-글루타민산나트륨제제, 면류첨가알칼리제, 보존료제제, 타르색소제제 등의 혼합 제제류 들을 들 수 있다.As items listed in the "Food Additives Codex", for example, chemical synthetic products such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid, natural additives such as depigmentation, licorice extract, crystalline cellulose, and guar gum, L- Mixed preparations such as sodium glutamate preparation, noodle-added alkali preparation, preservative preparation, and tar color preparation can be mentioned.
본 발명의 유효성분이 포함된 식품으로는 빵, 떡류, 건과류, 캔디류, 초콜릿류, 츄잉껌, 쨈류와 같은 과자류 아이스크림류, 빙과류, 아이스크림 분말류와 같은 아이스크림 제품류 우유류, 저지방 우유류, 유당분해 우유, 가공유류, 산양유, 발효유류, 버터유류, 농축유류, 유크림류, 버터유, 자연치즈, 가공치즈, 분유류, 유청류와 같은 유가공품류 식육가공품, 알가공품, 햄버거와 같은 식육제품류 어묵, 햄, 소시지, 베이컨 등의 어육가공품과 같은 어육제품류 라면류, 건면류, 생면류, 유탕면류, 호화건먼류, 개량숙면류, 냉동면류, 파스타류와 같은 면류 과실음료, 채소류음료, 탄산음료, 두유류, 요구르트 등의 유산균음료, 혼합음료와 같은 음료 간장, 된장, 고추장, 춘장, 청국장, 혼합장, 식초, 소스류, 토마토케첩, 카레, 드레싱과 같은 조미식품 마가린, 쇼트닝 및 피자를 들 수 있으나, 이에 한정되는 것은 아니다.Foods containing the active ingredient of the present invention include bread, rice cakes, dried fruits, candies, chocolates, chewing gum, confectionery products such as jams, ice cream products, ice cream products, ice cream products such as ice cream powder milk, low-fat milk, lactose-decomposed milk, Processed milk, goat milk, fermented milk, buttermilk, concentrated milk, milk cream, butter oil, natural cheese, processed cheese, milk powder, milk products such as whey Processed meat products, processed eggs, meat products such as hamburgers Fish cakes, ham, Fish and meat products such as sausages and processed fish meat products such as bacon Ramen noodles, dried noodles, raw noodles, fried noodles, luxurious dried noodles, improved soft noodles, frozen noodles, pasta, etc. Fruit drinks, vegetable drinks, carbonated drinks, soy milk, Lactic acid bacteria drinks such as yogurt, beverages such as mixed drinks Soy sauce, soybean paste, gochujang, chunjang, cheonggukjang, mixed soy sauce, vinegar, sauces, tomato ketchup, curry, seasoning foods such as dressings, margarine, shortening and pizza, but limited to this it's not going to be
상기 외에 본 발명의 조성물은 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 포함할 수 있다. 그 밖에 본 발명의 조성물은 천연 과일주스, 과일주스 음료 및 야채 음료의 제조를 위한 과육을 포함할 수 있다. 이러한 성분은 독립적으로 또는 조합하여 사용할 수 있다. In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, It may include a carbonation agent used in carbonated beverages, and the like. In addition, the composition of the present invention may include fruit for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. These components may be used independently or in combination.
본 발명의 유효성분을 포함한 음료 조성물은 다른 성분에는 특별한 제한이 없으며 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가성분으로 함유할 수 있다. 상술한 천연 탄수화물의 예는 모노사카라이드, (예를 들어, 포도당, 과당 등); 디사카라이드, (예를 들어 말토스, 슈크로스 등); 및 폴리사카라이드, (예를 들어 덱스트린, 시클로덱스트린 등)과 같은 통상적인 당, 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 상술한 것 이외의 향미제로서 천연 향미제(타우마틴, 스테비아 추출물(예를 들어 레바우디오시드 A, 글리시르히진 등) 및 합성 향미제(사카린, 아스파르탐 등)를 유리하게 사용할 수 있다.The beverage composition including the active ingredient of the present invention is not particularly limited in other ingredients, and may contain various flavoring agents or natural carbohydrates as additional ingredients like a conventional beverage. Examples of the aforementioned natural carbohydrates include monosaccharides (eg, glucose, fructose, etc.); disaccharides, (eg maltose, sucrose, etc.); and conventional sugars such as polysaccharides (eg, dextrin, cyclodextrin, etc.), and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatine, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. .
또한, 본 발명의 류코노스톡 시트래움 균주 또는 이의 배양물을 유효성분으로 포함하는 조성물은 가축의 장 손상 예방 또는 개선용 사료 조성물 또는 사료 첨가제 조성물로 이용될 수 있다.In addition, the composition comprising the Leukonostok citraum strain of the present invention or a culture thereof as an active ingredient may be used as a feed composition or feed additive composition for preventing or improving intestinal damage of livestock.
상기 조성물이 사료 첨가제로서 제조될 경우, 상기 조성물은 20 내지 90% 고농축액이거나 분말 또는 과립 형태로 제조될 수 있다. 상기 사료 첨가제는 구연산, 후말산, 아디픽산, 젖산, 사과산등의 유기산이나 인산 나트륨, 인산 칼륨, 산성 피로인산염, 폴리인산염(중합인산염) 등의 인산염이나, 폴리페놀, 카테킨, 알파-토코페롤, 로즈마리 추출물, 비타민 C, 녹차 추출물, 감초 추출물, 키토산, 탄닌산, 피틴산 등의 천연 항산화제 중 어느 하나 또는 하나 이상을 추가로 포함할 수 있다. 사료로서 제조될 경우, 상기 조성물은 통상의 사료 형태로 제제화될 수 있으며, 통상의 사료 성분을 함께 포함할 수 있다.When the composition is prepared as a feed additive, the composition may be 20 to 90% highly concentrated or may be prepared in powder or granular form. The feed additives include organic acids such as citric acid, humic acid, adipic acid, lactic acid, and malic acid, or phosphates such as sodium phosphate, potassium phosphate, acid pyrophosphate, polyphosphate (polyphosphate), polyphenol, catechin, alpha-tocopherol, rosemary Extract, vitamin C, green tea extract, licorice extract, chitosan, tannic acid, any one or more of natural antioxidants such as phytic acid may be further included. When prepared as a feed, the composition may be formulated in the form of a conventional feed, and may include common feed ingredients together.
상기 사료 및 사료 첨가제는 곡물, 예를 들면 분쇄 또는 파쇄된 밀, 귀리, 보리, 옥수수 및 쌀; 식물성 단백질 사료, 예를 들면 평지, 콩, 및 해바라기를 주성분으로 하는 사료; 동물성 단백질 사료, 예를 들면 혈분, 육분, 골분 및 생선분; 당분 및 유제품, 예를 들면 각종 분유 및 유장 분말로 이루어지는 건조성분 등을 더 포함할 수 있으며, 이외에도 영양보충제, 소화 및 흡수향상제, 성장촉진제 등을 더 포함할 수 있다.The feed and feed additives include grains such as milled or crushed wheat, oats, barley, corn and rice; plant protein feeds, such as feeds based on rape, soybean, and sunflower; animal protein feeds such as blood meal, meat meal, bone meal and fish meal; Sugar and dairy products, for example, may further include dry ingredients made of various powdered milk and whey powder, and may further include nutritional supplements, digestion and absorption enhancers, growth promoters, and the like.
상기 사료 첨가제는 동물에게 단독으로 투여하거나 식용 담체 중에서 다른 사료 첨가제와 조합하여 투여할 수도 있다. 또한, 상기 사료 첨가제는 탑드레싱으로서 또는 이들을 동물사료에 직접 혼합하거나 또는 사료와 별도의 경구제형으로 용이하게 동물에게 투여할 수 있다. 상기 사료 첨가제를 동물사료와 별도로 투여할 경우, 당해 기술분야에 잘 알려진 바와 같이 약제학적으로 허용 가능한 식용 담체와 조합하여, 즉시 방출 또는 서방성 제형으로 제조할 수 있다. 이러한 식용 담체는 고체 또는 액체, 예를 들어 옥수수전분, 락토오스, 수크로오스, 콩플레이크, 땅콩유, 올리브유, 참깨유 및 프로필렌글리콜일 수 있다. 고체 담체가 사용될 경우, 사료 첨가제는 정제, 캡슐제, 산제, 트로키제 또는 함당정제 또는 미분산성 형태의 탑 드레싱일 수 있다. 액체 담체가 사용될 경우, 사료 첨가제는 젤라틴 연질 캡슐제, 또는 시럽제나 현탁액, 에멀젼제, 또는 용액제의 제형일 수 있다.The feed additive may be administered to the animal alone or in combination with other feed additives in an edible carrier. In addition, the feed additive can be easily administered to the animal as a top dressing, directly mixing them with animal feed, or as an oral formulation separate from the feed. When the feed additive is administered separately from the animal feed, it may be combined with a pharmaceutically acceptable edible carrier as well known in the art to prepare an immediate release or sustained release formulation. Such edible carriers may be solid or liquid, for example cornstarch, lactose, sucrose, soybean flakes, peanut oil, olive oil, sesame oil and propylene glycol. When a solid carrier is used, the feed additive may be a tablet, capsule, powder, troche or sugar-containing tablet or top dressing in microdispersed form. When a liquid carrier is used, the feed additive may be in the form of a gelatin soft capsule, or a syrup or suspension, emulsion, or solution.
또한, 상기 사료 및 사료 첨가제는 보조제, 예를 들어 보존제, 안정화제, 습윤제 또는 유화제, 용액 촉진제 등을 함유할 수 있다. 상기 사료 첨가제는 침주, 분무 또는 혼합하여 동물의 사료에 첨가하여 이용될 수 있다.In addition, the feed and feed additives may contain adjuvants, for example, preservatives, stabilizers, wetting or emulsifying agents, solution accelerators, and the like. The feed additive may be used by being added to animal feed by immersion, spraying, or mixing.
본 발명의 사료 또는 사료 첨가제는 포유류, 가금 및 어류를 포함하는 다수의 동물 식이에 적용할 수 있다.The feed or feed additive of the present invention can be applied to a number of animal diets including mammals, poultry and fish.
상기 포유류로서 돼지, 소, 말, 양, 토끼, 염소, 설치동물 및 실험용 설치동물인 쥐, 햄스터, 기니피그 뿐만 아니라, 애완동물(예: 개, 고양이) 등에게 사용할 수 있으며, 상기 가금류로서 닭, 칠면조, 오리, 거위, 꿩, 및 메추라기 등에도 사용할 수 있고, 상기 어류로서 잉어, 붕어 및 송어 등에 이용될 수 있으나, 이에 한정되는 것은 아니다.Pigs, cattle, horses, sheep, rabbits, goats, rodents and experimental rodents as the mammals, as well as rats, hamsters, and guinea pigs, can be used for pets (eg, dogs, cats), etc., and as the poultry, chicken, It can also be used for turkey, duck, geese, pheasant, and quail, and the like, and may be used for carp, crucian carp and trout as the fish, but is not limited thereto.
실시예Example 1. 균주의 배양 1. Culture of the strain
류코노스톡 시트래움 WiKim0104(기탁번호 KCCM12420P) 균주는 기탁자인 한국식품연구원의 허락을 받아 분양받아 실험을 실시하였다.The Leukonostok Citraum WiKim0104 (Accession No. KCCM12420P) strain was tested with the permission of the Korea Food Research Institute, the depositor.
상기 분양받은 류코노스톡 시트래움 WiKim0104 균주를 MRS 액체배지 30ml에 1% 접종하여 30℃에서 18시간 동안 정치배양 하였다. 배양 후, 3000 rpm에서 10분 동안 원심분리하여 배양액은 따로 보관하였고, 균체는 PBS(phosphate buffered saline) 용액으로 3회 세척하여 남아 있는 배지 성분을 제거하였다.1% of the received Leukonostok Citraum WiKim0104 strain was inoculated into 30 ml of MRS broth, and cultured at 30° C. for 18 hours. After incubation, the culture medium was stored separately by centrifugation at 3000 rpm for 10 minutes, and the cells were washed three times with a phosphate buffered saline (PBS) solution to remove the remaining medium components.
실시예Example 2. 2. 장세포에서in enterocytes 장벽 유지 관련 유전자 발현 강화 효능 확인 Confirmation of the efficacy of enhancing barrier maintenance-related gene expression
장세포 (HT29 cell 및 Caco2) 배양을 위해 페니실린/스트렙토마이신, 10% FBS를 첨가한 RPMI 배지를 사용하였으며 6-웰 (6-well) 플레이트 트렌스웰을 이용하여 준비하였다. For culturing enterocytes (HT29 cells and Caco2), RPMI medium supplemented with penicillin/streptomycin and 10% FBS was used and prepared using a 6-well plate transwell.
장세포가 confluent하게 자랄 때까지 배양한 후에 LPS 1μg/ml와 팔미트산(Palmitic acid) 500μM가 포함된 200μl의 새로운 배지로 교환해준다. 이때 준비한 류코노스톡 시트래움 WiKim0104 균주를 1X108 CFU/mL의 농도로 처리한다. 상기 균주 처리 후 24시간 동안 배양한 뒤 PBS로 3번 세척하고 세포를 수확하여 TRIZOL 시약을 이용하여 RNA를 분리하였다. 1μg의 RNA를 cDNA 합성 kit를 이용하여 cDNA를 합성한 후 qRT-PCR(quantitative real time polymerase chain reaction) 방법으로 밀착연접(Tight junction) 관련 유전자인 ZO-1의 발현량을 확인한 결과를 도 1에 나타내었다.After culturing until enterocytes grow confluent, exchange with 200 μl of fresh medium containing 1 μg/ml of LPS and 500 μM of palmitic acid. At this time, the prepared Leukonostok Citraum WiKim0104 strain is treated at a concentration of 1X10 8 CFU/mL. After culturing for 24 hours after treating the strain, the cells were washed 3 times with PBS, harvested, and RNA was isolated using TRIZOL reagent. After synthesizing cDNA from 1 μg of RNA using a cDNA synthesis kit, the result of confirming the expression level of ZO-1, a tight junction-related gene, is shown in FIG. 1 by qRT-PCR (quantitative real time polymerase chain reaction). indicated.
도 1에 나타나는 바와 같이, 류코노스톡 시트래움 WiKim0104 균주 처리군에서 LPS와 팔미트산에 의해 감소된 ZO1의 발현량이 회복되는 결과를 보였다.As shown in FIG. 1 , in the Leukonostok Citraum WiKim0104 strain treatment group, the expression level of ZO1 decreased by LPS and palmitic acid was recovered.
실시예Example 3. 3. 비알콜성non-alcoholic 지방간염steatohepatitis 모델에서 in the model 장벽유지barrier maintenance 효능 확인 Efficacy Check
비알콜성 지방간염 또한 밀착연접(Tight junction)의 기능 장애에 밀접한 연관이 있는 것으로 알려져있다. 이에 choline deficient high fat diet(CDHFD) 방법으로 유도된 비알콜성 지방간염 모델에서 본 발명에 따른 조성물을 처리한 후 밀착연접 관련 유전자의 발현량을 측정하였다. 6~8주령 C57BL6 마우스를 CDHFD의 방법으로 8주간 섭취시켜 비알콜성 지방간염을 유도한 후 12주간 류코노스톡 시트래움 WiKim0104 균주를 2X109 CFU/day의 농도로 주 5일 급여하였다. 12주 후 대조군과 실험군의 장조직에서 밀착연접(Tight junction) 관련 유전자인 Occludin 및 ZO1 발현량을 확인한 결과를 도 2 및 도 3에 나타내었다.Nonalcoholic steatohepatitis is also known to be closely related to dysfunction of tight junctions. Accordingly, in a nonalcoholic steatohepatitis model induced by a choline deficient high fat diet (CDHFD) method, the expression level of close junction related genes was measured after treatment with the composition according to the present invention. 6-8 week old C57BL6 mice were ingested for 8 weeks by the CDHFD method to induce nonalcoholic steatohepatitis, and then the Leukonostok Citraum WiKim0104 strain was fed at a concentration of 2X10 9 CFU/day 5 days a week for 12 weeks. The results of confirming the expression levels of Occludin and ZO1, which are genes related to tight junctions, in the intestinal tissues of the control group and the experimental group after 12 weeks are shown in FIGS. 2 and 3 .
도 2 및 도 3에서 나타나는 바와 같이, 류코노스톡 시트래움 Wikim0104 균주를 섭취한 그룹에서 상기 Occludin 및 ZO1 단백질 유전자의 발현량이 정상군 수준으로 회복됨을 확인하였다. As shown in FIGS. 2 and 3 , it was confirmed that the expression levels of the Occludin and ZO1 protein genes were restored to the normal group level in the group consuming the Leukonostok Citraum Wikim0104 strain.
<110> Liscure Biosciences <120> Leuconostoc citreum WiKim0104 <130> WiKim0104 <150> KR 10-2021-0017813 <151> 2021-02-08 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1430 <212> DNA <213> Leuconostoc citreum <400> 1 gatgaacgct ggcggcgtgc ctaatacatg caagtcgaac gcgcagcgag aggtgcttgc 60 acctttcaag cgagtggcga acgggtgagt aacacgtgga taacctgcct caaggctggg 120 gataacattt ggaaacagat gctaataccg aataaaactt agtatcgcat gatatcaagt 180 taaaaggcgc tacggcgtca cctagagatg gatccgcggt gcattagtta gttggtgggg 240 taaaggctta ccaagacgat gatgcatagc cgagttgaga gactgatcgg ccacattggg 300 actgagacac ggcccaaact cctacgggag gctgcagtag ggaatcttcc acaatgggcg 360 caagcctgat ggagcaacgc cgcgtgtgtg atgaaggctt tcgggtcgta aagcactgtt 420 gtatgggaag aaatgctaaa atagggaatg attttagttt gacggtacca taccagaaag 480 ggacggctaa atacgtgcca gcagccgcgg taatacgtat gtcccgagcg ttatccggat 540 ttattgggcg taaagcgagc gcagacggtt gattaagtct gatgtgaaag cccggagctc 600 aactccggaa tggcattgga aactggttaa cttgagtgtt gtagaggtaa gtggaactcc 660 atgtgtagcg gtggaatgcg tagatatatg gaagaacacc agtggcgaag gcggcttact 720 ggacaacaac tgacgttgag gctcgaaagt gtgggtagca aacaggatta gataccctgg 780 tagtccacac cgtaaacgat gaatactagg tgttaggagg tttccgcctc ttagtgccga 840 agctaacgca ttaagtattc cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa 900 ttgacgggga cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac 960 cttaccaggt cttgacatcc tttgaagctt ttagagatag aagtgttctc ttcggagaca 1020 aagtgacagg tggtgcatgg tcgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080 gcaacgagcg caacccttat tgttagttgc cagcattcag ttgggcactc tagcgagact 1140 gccggtgaca aaccggagga aggcggggac gacgtcagat catcatgccc cttatgacct 1200 gggctacaca cgtgctacaa tggcgtatac aacgagttgc caacctgcga aggtgagcta 1260 atctcttaaa gtacgtctca gttcggactg cagtctgcaa ctcgactgca cgaagtcgga 1320 atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggtc ttgtacacac 1380 cgcccgtcac accatgggag tttgtaatgc ccaaagccgg tggcctaacc 1430 <110> Liscure Biosciences <120> Leuconostoc citreum WiKim0104 <130> WiKim0104 <150> KR 10-2021-0017813 <151> 2021-02-08 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1430 <212> DNA <213> Leuconostoc citreum <400> 1 gatgaacgct ggcggcgtgc ctaatacatg caagtcgaac gcgcagcgag aggtgcttgc 60 acctttcaag cgagtggcga acgggtgagt aacacgtgga taacctgcct caaggctggg 120 gataacattt ggaaacagat gctaataccg aataaaactt agtatcgcat gatatcaagt 180 taaaaggcgc tacggcgtca cctagagatg gatccgcggt gcattagtta gttggtgggg 240 taaaggctta ccaagacgat gatgcatagc cgagttgaga gactgatcgg ccacattggg 300 actgagacac ggcccaaact cctacgggag gctgcagtag ggaatcttcc acaatgggcg 360 caagcctgat ggagcaacgc cgcgtgtgtg atgaaggctt tcgggtcgta aagcactgtt 420 gtatgggaag aaatgctaaa atagggaatg attttagttt gacggtacca taccagaaag 480 ggacggctaa atacgtgcca gcagccgcgg taatacgtat gtcccgagcg ttatccggat 540 ttattgggcg taaagcgagc gcagacggtt gattaagtct gatgtgaaag cccggagctc 600 aactccggaa tggcattgga aactggttaa cttgagtgtt gtagaggtaa gtggaactcc 660 atgtgtagcg gtggaatgcg tagatatatg gaagaacacc agtggcgaag gcggcttact 720 ggacaacaac tgacgttgag gctcgaaagt gtgggtagca aacaggatta gataccctgg 780 tagtccacac cgtaaacgat gaatactagg tgttaggagg tttccgcctc ttagtgccga 840 agctaacgca ttaagtattc cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa 900 ttgacgggga cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac 960 cttaccaggt cttgacatcc tttgaagctt ttagagatag aagtgttctc ttcggagaca 1020 aagtgacagg tggtgcatgg tcgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080 gcaacgagcg caacccttat tgttagttgc cagcattcag ttgggcactc tagcgagact 1140 gccggtgaca aaccggagga aggcggggac gacgtcagat catcatgccc cttatgacct 1200 gggctacaca cgtgctacaa tggcgtatac aacgagttgc caacctgcga aggtgagcta 1260 atctcttaaa gtacgtctca gttcggactg cagtctgcaa ctcgactgca cgaagtcgga 1320 atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggtc ttgtacacac 1380 cgcccgtcac accatgggag tttgtaatgc ccaaagccgg tggcctaacc 1430
Claims (12)
상기 류코노스톡 시트래움 균주는 서열번호 1의 핵산서열을 갖는, 류코노스톡 시트래움 WiKim0104 (Leuconostoc citreum WiKim0104; 수탁번호 KCCM12420P) 균주인, 장 손상의 예방 또는 치료용 약학 조성물.According to claim 1,
The leuconostoc citreum strain has the nucleic acid sequence of SEQ ID NO: 1, Leuconostoc citreum WiKim0104 (Leuconostoc citreum WiKim0104; accession number KCCM12420P) strain, a pharmaceutical composition for preventing or treating intestinal damage.
상기 장 손상은 염증성 장질환, 비알콜성 지방간염 또는 항암 치료로 인한 장 손상인, 장 손상의 예방 또는 치료용 약학 조성물. According to claim 1,
The intestinal damage is inflammatory bowel disease, non-alcoholic steatohepatitis, or intestinal damage caused by anticancer treatment, a pharmaceutical composition for the prevention or treatment of intestinal damage.
상기 조성물은 경구 투여 또는 비경구 투여의 방법으로 투여 가능한 것을 특징으로 하는 장 손상의 예방 또는 치료용 약학 조성물. According to claim 1,
The composition is a pharmaceutical composition for preventing or treating intestinal damage, characterized in that it can be administered by oral administration or parenteral administration.
상기 비경구 투여의 방법은 정맥 내 주입, 피하 주입, 근육 주입, 복강 주입, 내피 투여, 국소 투여, 비내 투여, 폐 내 투여 또는 직장 내 투여인 것인, 장 손상의 예방 또는 치료용 약학 조성물.5. The method of claim 4,
The method of parenteral administration is intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, endothelial administration, topical administration, intranasal administration, intrapulmonary administration or rectal administration, a pharmaceutical composition for preventing or treating intestinal damage.
상기 식품은 건강기능식품인, 장 손상의 예방 또는 개선용 식품 조성물.7. The method of claim 6,
The food is a health functional food, a food composition for preventing or improving intestinal damage.
상기 식품은 빵, 떡, 캔디, 초콜릿, 껌, 아이스크림, 우유, 치즈, 식육가공품, 어육가공품, 김치, 간장, 된장, 고추장, 춘장, 청국장, 식초, 케첩, 카레, 드레싱, 음료 및 발효유로 이루어지는 군으로부터 선택되는 어느 하나의 식품인 것을 특징으로 하는 장 손상의 예방 또는 개선용 식품 조성물.7. The method of claim 6,
The food is bread, rice cake, candy, chocolate, gum, ice cream, milk, cheese, processed meat products, fish meat products, kimchi, soy sauce, soybean paste, gochujang, chunjang, cheonggukjang, vinegar, ketchup, curry, dressing, beverage and fermented milk consisting of Food composition for preventing or improving intestinal damage, characterized in that any one food selected from the group.
상기 가축은 돼지, 소, 말, 양, 토끼, 염소, 쥐, 햄스터, 기니피그, 개, 고양이, 닭, 칠면조, 오리, 거위, 꿩, 메추라기, 잉어, 붕어 및 송어로 이루어지는 군으로부터 선택되는 어느 하나인 것인, 가축의 장 손상 예방 또는 개선용 사료 조성물.11. The method of claim 10,
The livestock is any one selected from the group consisting of pig, cow, horse, sheep, rabbit, goat, rat, hamster, guinea pig, dog, cat, chicken, turkey, duck, goose, pheasant, quail, carp, crucian carp and trout which is, a feed composition for preventing or improving intestinal damage in livestock.
A feed additive composition for preventing or improving intestinal damage of livestock comprising a Leuconostoc citreum strain or a culture thereof as an active ingredient.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA3207736A CA3207736A1 (en) | 2021-02-08 | 2022-02-07 | Pharmaceutical composition for preventing or treating intestinal damage comprising leuconostoc citreum strain as active ingredient |
| US18/276,154 US20240091281A1 (en) | 2021-02-08 | 2022-02-07 | Pharmaceutical composition for preventing or treating intestinal damage comprising leuconostoc citreum strain as active ingredient |
| JP2023547880A JP2024506051A (en) | 2021-02-08 | 2022-02-07 | Pharmaceutical composition for preventing or treating intestinal damage containing Leuconostoc citreum strain as an active ingredient |
| CN202280014024.5A CN117337187A (en) | 2021-02-08 | 2022-02-07 | Pharmaceutical composition for preventing or treating intestinal injury comprising Leuconostoc citreum strain as active ingredient |
| PCT/KR2022/001870 WO2022169337A1 (en) | 2021-02-08 | 2022-02-07 | Pharmaceutical composition comprising leuconostoc citreum strain as active ingredient for prevention or treatment of intestinal damage |
| EP22750086.5A EP4289440A1 (en) | 2021-02-08 | 2022-02-07 | Pharmaceutical composition comprising leuconostoc citreum strain as active ingredient for prevention or treatment of intestinal damage |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020210017813 | 2021-02-08 | ||
| KR20210017813 | 2021-02-08 |
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| Publication Number | Publication Date |
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| KR20220114490A true KR20220114490A (en) | 2022-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| KR1020220014825A KR20220114490A (en) | 2021-02-08 | 2022-02-04 | Pharmaceutical composition for prevention or treatment of intestinal damage comprising Leuconostoc citreum as active ingredient |
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| KR (1) | KR20220114490A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102038695B1 (en) | 2018-10-30 | 2019-10-30 | 주식회사 종근당바이오 | Composition for Preventing or Treating Alcoholic Intestinal Damage Comprising Probiotics as Effective Ingredients |
| KR102201517B1 (en) | 2019-12-11 | 2021-01-13 | 주식회사 에이치이엠 | A novel strain of Lactobacillus acidophilus HEM 960, and composition for improving gut environment comprising the strain or its culture fluid |
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- 2022-02-04 KR KR1020220014825A patent/KR20220114490A/en unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102038695B1 (en) | 2018-10-30 | 2019-10-30 | 주식회사 종근당바이오 | Composition for Preventing or Treating Alcoholic Intestinal Damage Comprising Probiotics as Effective Ingredients |
| KR102201517B1 (en) | 2019-12-11 | 2021-01-13 | 주식회사 에이치이엠 | A novel strain of Lactobacillus acidophilus HEM 960, and composition for improving gut environment comprising the strain or its culture fluid |
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