KR20220100260A - Effective extraction method of pepper extract containing high polyamine having anti-inflammatory activity - Google Patents
Effective extraction method of pepper extract containing high polyamine having anti-inflammatory activity Download PDFInfo
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- KR20220100260A KR20220100260A KR1020210002493A KR20210002493A KR20220100260A KR 20220100260 A KR20220100260 A KR 20220100260A KR 1020210002493 A KR1020210002493 A KR 1020210002493A KR 20210002493 A KR20210002493 A KR 20210002493A KR 20220100260 A KR20220100260 A KR 20220100260A
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- acid
- extract
- present
- inflammation
- functional food
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Abstract
Description
본 발명은 항염증 활성을 갖는 폴리아민 고함유 고추 추출물의 유효 추출 방법에 관한 것이다.The present invention relates to an effective extraction method of a red pepper extract having a high polyamine content having anti-inflammatory activity.
본 발명은 항염증 활성을 갖는 폴리아민 고함유 고추 추출물의 유효 추출 방법에 관한 것이다.The present invention relates to an effective extraction method of a red pepper extract having a high polyamine content having anti-inflammatory activity.
폴리아민(polyamine)은 amine group(-NH2)이 두개 이상 가지고 있는 carbohydrate chain이다. 모든 세포에 분포하며 대사에 있어서 다양한 역할을 수행하는 양이온(cation) 저 분자 물질이다. 대표적인 폴리아민(polyamine)으로는 푸트레신(putrescine), 스퍼미딘(spermidine) 및 스퍼민(spermine)이 있으며, 이러한 외인성 폴리아민(polyamine)의 경우 섭취에 따라 정상적인 대사에 있어서 다양한 효능을 나타낸다.A polyamine is a carbohydrate chain containing two or more amine groups (-NH2). It is a low-molecular cation that is distributed in all cells and performs various roles in metabolism. Representative polyamines include putrescine, spermidine, and spermine, and in the case of these exogenous polyamines, they exhibit various effects in normal metabolism according to ingestion.
추출(Extraction)이란 폴리아민(polyamine)과 같은 저 분자 아민(amine)류 물질을 추출하기 위해서는 산 용액을 통한 가수 분해법(hydrolysis method)가 주로 이용된다. 특히, 대표적인 산(acid)인 트라이클로로아세트산(Trichloroacetic acid, TCA) 와 과염소산(perchloric acid)가 주로 사용되며 추출되는 조직이나 대상에 따라서 다양한 산 용액이 사용된다.Extraction is a hydrolysis method using an acid solution to extract low molecular weight amines such as polyamines. In particular, trichloroacetic acid (TCA) and perchloric acid, which are representative acids, are mainly used, and various acid solutions are used depending on the tissue or subject to be extracted.
산화질소(Nitric oxide, NO)는 외부 자극에 의해 대식세포(macrophage)가 활성화가 되며 iNOS(inducible nitric oxide synthase) 조절에 의해 세포 외로 분비되는 물질이다. 염증 반응의 marker로서 griess A/B 용액과 아조 커플링 반응(azo coupling reaction)을 통해서 특정 파장에서 흡광한다.Nitric oxide (NO) is a substance secreted to the outside of cells by the activation of macrophages by external stimulation and the regulation of inducible nitric oxide synthase (iNOS). As a marker of an inflammatory response, it absorbs light at a specific wavelength through an azo coupling reaction with griess A/B solution.
염증 사이토카인(Inflammation cytokines)은 대표적으로 TNF-a, IL-1b, IL-6 등이 있으며 외부 자극에 의해 활성화된 대식세포(macrophage)에 의해 분비된다. 분비된 사이토카인(cytokines)들은 다른 림프구(lymphocytes)를 자극하여 염증 반응을 유도한다.Inflammation cytokines are typically TNF-a, IL-1b, IL-6, and the like, and are secreted by macrophages activated by external stimuli. The secreted cytokines stimulate other lymphocytes to induce an inflammatory response.
본 발명은 생리적 활성 저 분자 물질인 폴리아민(polyamine)을 고추(pepper)로부터 고농도로 추출하는 추출 조건을 최적화한 것이다. 최적화에 사용된 추출 용매는 종류 별 acid로 진행했으며, 이 중에서 폴리아민(polyamine)을 다량으로 함유한 조건을 선정하였다. 또한, 항염증(Anti-inflammation) 활성을 평가하기 위해 추출된 고추 추출물을 농도 별로 mouse macrophage(RAW264.7)에 처리하여 nitric oxide 함량과 inflammation cytokine gene의 유전자 발현 양상을 관찰하여 뛰어난 항염증 효과를 확인하여 본 발명을 완성하였다.The present invention optimizes the extraction conditions for extracting polyamine, which is a physiologically active low-molecular substance, from red pepper (pepper) at a high concentration. The extraction solvent used for optimization was carried out with acids for each type, and a condition containing a large amount of polyamine was selected among them. In addition, in order to evaluate the anti-inflammation activity, the extracted red pepper extract was treated with mouse macrophage (RAW264.7) by concentration to observe the nitric oxide content and the gene expression pattern of the inflammation cytokine gene. By confirming, the present invention was completed.
본 발명은 상기와 같은 문제를 해결하기 위해, 항염증 활성을 갖는 폴리아민 고함유 고추 추출물의 유효 추출 방법을 제공한다. 또한, 상기 추출 방법에 의해 제조된 추출물을 포함하는 염증 예방 또는 개선용 건강기능식품 조성물과 화장료 조성물을 제공한다.The present invention provides an effective extraction method of a red pepper extract having a high polyamine content having anti-inflammatory activity in order to solve the above problems. In addition, it provides a health functional food composition and cosmetic composition for preventing or improving inflammation comprising the extract prepared by the extraction method.
상기한 과제를 해결하기 위하여, 본 발명은 고추(pepper) 추출물을 유효성분으로 포함하는, 염증 예방 또는 개선용 건강기능식품 조성물을 제공한다.In order to solve the above problems, the present invention provides a health functional food composition for preventing or improving inflammation, comprising a red pepper (pepper) extract as an active ingredient.
본 발명의 일 실시예에 있어서, 본 발명의 “추출물”은 산(acid)를 이용하여 추출한 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the "extract" of the present invention may be characterized in that it is extracted using an acid, but is not limited thereto.
본 발명의 일 실시예에 있어서, 본 발명의 “산(acid)”은 염산(HCl), 과염소산(Perchloric acid), 인산(Phosphoric acid), 아세트산(Acetic acid), 시트르산(Citric acid) 및 폼산(Formic acid) 중 어느 하나인 것을 특징으로 할 수 있고, 바람직하게는 0.1 % 염산(HCl)을 이용할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the "acid" of the present invention is hydrochloric acid (HCl), perchloric acid, phosphoric acid, acetic acid, citric acid, and formic acid ( Formic acid) may be characterized in any one, preferably 0.1% hydrochloric acid (HCl) may be used, but is not limited thereto.
본 발명의 일 실시예에 있어서, 본 발명의 “추출물”은 푸트레신(putrescine), 스퍼미딘(spermidine) 및 스퍼민(spermine)을 함유하고 있는 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the "extract" of the present invention may be characterized as containing putrescine, spermidine and spermine, but is not limited thereto.
본 발명의 일 실시예에 있어서, 본 발명의 “조성물”은 산화질소(NO) 저해 활성을 가지는 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the "composition" of the present invention may be characterized as having a nitric oxide (NO) inhibitory activity, but is not limited thereto.
본 발명의 일 실시예에 있어서, 본 발명의 “조성물”은 염증성 사이토카인 TNF-a, IL-1b 및 IL-6의 발현을 억제시키는 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the "composition" of the present invention may be characterized by inhibiting the expression of inflammatory cytokines TNF-a, IL-1b and IL-6, but is not limited thereto.
또한, 본 발명은 고추(pepper) 추출물을 유효성분으로 포함하는, 염증 예방 또는 개선용 화장료 조성물에서, 상기 추출물은 산(acid)를 이용하여 추출한 것을 특징으로 하고, 상기 산(acid)는 염산(HCl), 과염소산(Perchloric acid), 인산(Phosphoric acid), 아세트산(Acetic acid), 시트르산(Citric acid) 및 폼산(Formic acid) 중 어느 하나인 것을 특징으로 하는, 염증 예방 또는 개선용 화장료 조성물을 제공한다.In addition, the present invention is a cosmetic composition for preventing or improving inflammation comprising a red pepper (pepper) extract as an active ingredient, the extract is characterized in that the extract is extracted using an acid, the acid (acid) is hydrochloric acid ( HCl), perchloric acid, phosphoric acid, acetic acid, citric acid, and formic acid, characterized in that any one of, it provides a cosmetic composition for preventing or improving inflammation do.
본 발명은 생리적 활성 저 분자 물질인 폴리아민(polyamine)을 고추(pepper)로부터 고농도로 추출하는 추출 조건을 최적화한 것이다. 최적화에 사용된 추출 용매는 종류 별 acid로 진행했으며, 이 중에서 폴리아민(polyamine)을 다량으로 함유한 조건을 선정하였다. 또한, 항염증(Anti-inflammation) 활성을 평가하기 위해 추출된 고추 추출물을 농도 별로 mouse macrophage(RAW264.7)에 처리하여 nitric oxide 함량과 inflammation cytokine gene의 유전자 발현 양상을 관찰하여 뛰어난 항염증 효과를 확인하여 본 발명을 완성하였다. 이는 염증 예방 또는 개선용 건강기능식품 조성물과 염증 예방 또는 개선용 화장료 조성물로 유용하게 사용될 수 있다.The present invention optimizes the extraction conditions for extracting polyamine, which is a physiologically active low-molecular substance, from red pepper (pepper) at a high concentration. The extraction solvent used for optimization was carried out with acids for each type, and a condition containing a large amount of polyamine was selected among them. In addition, in order to evaluate the anti-inflammation activity, the extracted red pepper extract was treated with mouse macrophage (RAW264.7) by concentration to observe the nitric oxide content and the gene expression pattern of the inflammation cytokine gene. By confirming, the present invention was completed. It can be usefully used as a health functional food composition for preventing or improving inflammation and a cosmetic composition for preventing or improving inflammation.
도 1은 추출 용매 별 용매 별 polyamine 함량을 비교한 도이다.
도 2는 추출 용매 별 NO production 저해 활성(a)과 cell viability(b) 평가를 나타낸 도이다.
도 3은 0.1% HCl extract의 농도 별 NO production 저해 활성(a)과 cell viability(b) 평가를 나타낸 도이다.
도 4는 염증성 cytokines mRNA 발현 변화량을 측정한 도이다(a; TNF-a, b; IL-1b , c; IL-6).1 is a diagram comparing the polyamine content of each solvent for each extraction solvent.
2 is a diagram showing the evaluation of NO production inhibitory activity (a) and cell viability (b) for each extraction solvent.
Figure 3 is a view showing the NO production inhibitory activity (a) and cell viability (b) evaluation for each concentration of 0.1% HCl extract.
Figure 4 is a diagram measuring the amount of inflammatory cytokines mRNA expression (a; TNF-a, b; IL-1b, c; IL-6).
본 발명은 고추(pepper) 추출물을 유효성분으로 포함하는, 염증 예방 또는 개선용 건강기능식품 조성물을 제공하는 것을 목적으로 한다.An object of the present invention is to provide a health functional food composition for preventing or improving inflammation, comprising a red pepper (pepper) extract as an active ingredient.
본 발명은 고추(pepper) 추출물을 유효성분으로 포함하는, 염증 예방 또는 개선용 화장료 조성물을 제공하는 것을 목적으로 한다.An object of the present invention is to provide a cosmetic composition for preventing or improving inflammation, comprising a pepper extract as an active ingredient.
본 발명은 항염증 활성을 갖는 폴리아민 고함유 고추 추출물의 유효 추출 방법을 제공하는 것을 목적으로 한다.An object of the present invention is to provide an effective extraction method of a red pepper extract having a high polyamine content having anti-inflammatory activity.
이하, 첨부된 도면을 참조하여 본 발명의 구현예로 본 발명을 상세히 설명하기로 한다. 다만, 하기 구현 예는 본 발명에 대한 예시로 제시되는 것으로, 당업자에게 주지 저명한 기술 또는 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명을 생략할 수 있고, 이에 의해 본 발명이 제한되지는 않는다. 본 발명은 후술하는 특허 청구범위의 기재 및 그로부터 해석되는 균등 범주 내에서 다양한 변형 및 응용이 가능하다.Hereinafter, the present invention will be described in detail by way of embodiments of the present invention with reference to the accompanying drawings. However, the following embodiments are presented as examples of the present invention, and when it is determined that detailed descriptions of well-known techniques or configurations known to those skilled in the art may unnecessarily obscure the gist of the present invention, the detailed description may be omitted. , the present invention is not limited thereby. Various modifications and applications of the present invention are possible within the scope of equivalents interpreted therefrom and the description of the claims to be described later.
또한, 본 명세서에서 사용되는 용어(terminology)들은 본 발명의 바람직한 실시 예를 적절히 표현하기 위해 사용된 용어들로서, 이는 사용자, 운용자의 의도 또는 본 발명이 속하는 분야의 관례 등에 따라 달라질 수 있다. 따라서 본 용어들에 대한 정의는 본 명세서 전반에 걸친 내용을 토대로 내려져야 할 것이다. 명세서 전체에서, 어떤 부분이 어떤 구성요소를 “포함”한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성요소를 제외하는 것이 아니라 다른 구성 요소를 더 포함할 수 있는 것을 의미한다.In addition, the terms used in this specification are terms used to properly express a preferred embodiment of the present invention, which may vary according to the intention of a user or operator, or a custom in the field to which the present invention belongs. Therefore, definitions of these terms should be made based on the content throughout this specification. Throughout the specification, when a part “includes” a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.
본 명세서 전체에 걸쳐, 특정 물질의 농도를 나타내기 위하여 사용되는 '%'는 별도의 언급이 없는 경우, 고체/고체는(w/w) %, 고체/액체는(w/v) %, 그리고 액체/액체는(v/v) %이다.Throughout this specification, '%' used to indicate the concentration of a specific substance is solid/solid (w/w) %, solid/liquid (w/v) %, and Liquid/liquid is (v/v) %.
일 측면에서, 고추(pepper) 추출물을 유효성분으로 포함하는, 염증 예방 또는 개선용 건강기능식품 조성물에 관한 것이다.In one aspect, it relates to a health functional food composition for preventing or improving inflammation, comprising a red pepper (pepper) extract as an active ingredient.
본 발명의 건강기능식품 조성물은 유효성분인 추출물을 함유하는 것 외에 통상의 식품 조성물과 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다.The health functional food composition of the present invention may contain various flavoring agents or natural carbohydrates as additional ingredients like a conventional food composition in addition to containing the extract as an active ingredient.
상술한 천연 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스 등; 및 폴리사카라이드, 예를 들어 덱스트린, 시클로덱스트린 등과 같은 통상적인 당, 및 자일리톨,소르비톨, 에리트리톨 등의 당알콜이다. 상술한 향미제는 천연 향미제(타우마틴), 스테비아 추출물(예를 들어 레바우디오시드 A, 글리시르히진 등) 및 합성 향미제(사카린, 아스파르탐 등)를 유리하게 사용할 수 있다. 본 발명의 식품 조성물은 상기 약학적 조성물과 동일한 방식으로 제제화되어 기능성 식품으로 이용하거나, 각종 식품에 첨가할 수 있다. 본 발명의 조성물을 첨가할 수 있는 식품으로는 예를 들어, 음료류, 육류, 초코렛, 식품류, 과자류, 피자, 라면, 기타 면류, 껌류, 사탕류, 아이스크림류, 알코올 음료류, 비타민 복합제 및 건강보조식품류 등이 있다.Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. The above-described flavoring agent may advantageously use a natural flavoring agent (taumatine), a stevia extract (eg, rebaudioside A, glycyrrhizin, etc.), and a synthetic flavoring agent (saccharin, aspartame, etc.). The food composition of the present invention may be formulated in the same manner as the pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, meat, chocolate, foods, confectionery, pizza, ramen, other noodles, gums, candy, ice cream, alcoholic beverages, vitamin complexes and health supplements. There is this.
또한, 상기 식품 조성물은 유효성분인 추출물 외에 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. 그밖에 본 발명의 식품 조성물은 천연 과일 쥬스 및 과일 쥬스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다.In addition, the food composition contains various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavoring agents, colorants and thickeners (cheese, chocolate, etc.), pectic acid and salts thereof, in addition to the active ingredient extract. , alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the food composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages.
본 발명의 기능성 식품 조성물은, 정제, 캅셀, 분말, 과립, 액상, 환 등의 형태로 제조 및 가공될 수 있다. 본 발명에서 '건강기능식품 조성물'이라 함은 건강기능식품에 관한 법률 제6727호에 따른 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 제조 및 가공한 식품을 말하며, 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건용도에 유용한 효과를 얻을 목적으로 섭취하는 것을 의미한다. 본 발명의 건강 기능성 식품은 통상의 식품 첨가물을 포함할 수 있으며, 식품 첨가물로서의 적합 여부는 다른 규정이 없는 한, 식품의약품안전청에 승인된 식품 첨가물 공전의 총칙 및 일반시험법 등에 따라 해당 품목에 관한 규격 및 기준에 의하여 판정한다. 상기 '식품 첨가물 공전'에 수재된 품목으로는 예를 들어, 케톤류, 글리신, 구연산칼슘, 니코틴산, 계피산 등의 화학적 합성물; 감색소, 감초추출물, 결정셀룰로오스, 고량색소, 구아검 등의 천연첨가물; L-글루타민산나트륨 제제, 면류첨가알칼리제, 보존료 제제, 타르색소제제 등의 혼합제제류 등을 들 수 있다. 예를 들어, 정제 형태의 건강 기능성 식품은 본 발명의 유효성분을 부형제, 결합제, 붕해제 및 다른 첨가제와 혼합한 혼합물을 통상의 방법으로 과립화한 다음, 활택제 등을 넣어 압축성형하거나, 상기 혼합물을 직접 압축 성형할 수 있다. 또한 상기 정제 형태의 건강 기능성 식품은 필요에 따라 교미제 등을 함유할 수도 있다. 캅셀 형태의 건강 기능성 식품 중 경질 캅셀제는 통상의 경질 캅셀에 본 발명의 유효성분을 부형제 등의 첨가제와 혼합한 혼합물을 충진하여 제조할 수 있으며, 연질 캅셀제는 본 발명의 유효성분을 부형제 등의 첨가제와 혼합한 혼합물을 젤라틴과 같은 캅셀기제에 충진하여 제조할 수 있다. 상기 연질 캅셀제는 필요에 따라 글리세린 또는 소르비톨 등의 가소제, 착색제, 보존제 등을 함유할 수 있다. 환 형태의 건강 기능성 식품은 본 발명의 유효성분과 부형제, 결합제, 붕해제 등을 혼합한 혼합물을 기존에 공지된 방법으로 성형하여 조제할 수 있으며, 필요에 따라 백당이나 다른 제피제로 제피할 수 있으며, 또는 전분, 탈크와 같은 물질로 표면을 코팅할 수도 있다. 과립 형태의 건강 기능성 식품은 본 발명의 유효성분의 부형제, 결합제, 붕해제 등을 혼합한 혼합물을 기존에 공지된 방법으로 입상으로 제조할 수 있으며, 필요에 따라 착향제, 교미제 등을 함유할 수 있다.The functional food composition of the present invention may be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, and the like. In the present invention, the term 'health functional food composition' refers to food manufactured and processed using raw materials or ingredients useful for the human body according to Act No. 6727 of the Health Functional Food Act, and It refers to intake for the purpose of obtaining useful effects for health purposes, such as regulating nutrients or physiological effects. The health functional food of the present invention may contain general food additives, and unless otherwise specified, whether it is suitable as a food additive is related to the item according to the general rules and general test method of food additives approved by the Food and Drug Administration. It is judged according to the standards and standards. The items listed in the 'Food Additives Code' include, for example, chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; natural additives such as persimmon pigment, licorice extract, crystalline cellulose, high pigment, and guar gum; Mixed preparations, such as a sodium L-glutamate preparation, a noodle-added alkali agent, a preservative preparation, and a tar dye preparation, etc. are mentioned. For example, a health functional food in tablet form is granulated by a conventional method by mixing the active ingredient of the present invention with an excipient, a binder, a disintegrant and other additives, followed by compression molding by adding a lubricant, etc., or The mixture can be compression molded directly. In addition, the health functional food in the form of tablets may contain a corrosive agent or the like, if necessary. Among health functional foods in the form of capsules, hard capsules can be prepared by filling a mixture of the active ingredient of the present invention with additives such as excipients in ordinary hard capsules. It can be prepared by filling a mixture mixed with a capsule base such as gelatin. The soft capsules may contain a plasticizer such as glycerin or sorbitol, a colorant, a preservative, and the like, if necessary. A health functional food in the form of a ring can be prepared by molding a mixture of the active ingredient of the present invention and an excipient, a binder, a disintegrant, etc. by a known method, and if necessary, it can be coated with sucrose or other skinning agent, Alternatively, the surface may be coated with a material such as starch or talc. A health functional food in the form of granules can be prepared in a granular form by a conventionally known method by mixing a mixture of an excipient, a binder, a disintegrant, etc. of the active ingredient of the present invention, and may contain a flavoring agent, a flavoring agent, etc., if necessary. can
일 측면에서, 고추(pepper) 추출물을 유효성분으로 포함하는, 염증 예방 또는 개선용 화장료 조성물에 관한 것이다.In one aspect, it relates to a cosmetic composition for preventing or improving inflammation, comprising a red pepper (pepper) extract as an active ingredient.
본 발명의 “화장료 조성물”은 상술한 본 발명의 고추(pepper)에서 추출한 추출물의 화장품학적 유효량(cosmetically effective amount) 및 화장품학적으로 허용되는 담체를 포함하여 제조할 수 있다.The "cosmetics composition" of the present invention can be prepared by including a cosmetically effective amount of the extract extracted from pepper of the present invention and a cosmetically acceptable carrier.
본 명세서에서 용어 “화장품학적 유효량”은 상술한 본 발명의 조성물의 항염증 효능을 달성하는 데 충분한 양을 의미한다.As used herein, the term “cosmetic effective amount” means an amount sufficient to achieve the anti-inflammatory effect of the composition of the present invention described above.
화장료 조성물의 외형은 화장품학 또는 피부과학적으로 허용 가능한 매질 또는 기제를 함유한다. 이는 국소적용에 적합한 모든 제형으로, 예를 들면, 용액, 겔, 고체, 반죽 무수 생성물, 수상에 유상을 분산시켜 얻은 에멀젼, 현탁액, 마이크로에멀젼, 마이크로캡슐, 미세과립구 또는, 이온형(리포좀) 및 비이온형의 소낭 분산제의 형태로, 또는 크림, 스킨, 로션, 파우더, 연고, 스프레이 또는 콘실 스틱의 형태로 제공될 수 있다. 이들 조성물은 당해 분야의 통상적인 방법에 따라 제조될 수 있다. 본 발명에 따른 조성물은 또한 포말(foam)의 형태로 또는 압축된 추진제를 더 함유한 에어로졸 조성물의 형태로도 사용될 수 있다.The appearance of the cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. It is in any dosage form suitable for topical application, e.g. solutions, gels, solids, kneaded dry products, emulsions obtained by dispersing the oily phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or, ionic (liposomes) and It may be provided in the form of a non-ionic vesicular dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or concealer stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.
본 발명의 일 실시예에 따른 상기 화장료 조성물은 그 제형에 있어서 특별히 한정되는 바가 없으며, 예를 들면, 유연화장수, 수렴화장수, 영양화장수, 영양크림, 마사지크림, 에센스, 아이크림, 아이에센스, 클렌징크림, 클렌징폼, 클렌징워터, 팩, 파우더, 바디로션, 바디크림, 바디오일 및 바디에센스 등의 화장품으로 제형화될 수 있다.The cosmetic composition according to an embodiment of the present invention is not particularly limited in its formulation, for example, softening lotion, astringent lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing It can be formulated into cosmetics such as cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil, and body essence.
본 발명의 화장료 조성물의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물섬유, 식물섬유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a paste, cream, or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. This can be used.
본 발명의 화장료 조성물의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro It may contain a propellant such as carbon, propane/butane or dimethyl ether.
본 발명의 화장료 조성물의 제형이 용액 또는 유탁액의 경우에는 담체 성분으로서 용매, 용매화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.
본 발명의 화장료 조성물의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used.
본 발명의 화장료 조성물의 제형이 계면-활성제 함유 클린징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 리놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate as carrier components , fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester, and the like can be used.
본 발명의 화장료 조성물은 스킨, 로션, 크림, 에센스, 팩, 파운데이션, 색조화장품, 선크림, 투웨이케이크, 페이스파우더, 콤팩트, 메이크업베이스, 스킨커버, 아이쉐도우, 립스틱, 립글로스, 립픽스, 아이브로우 펜슬, 화장수 등의 화장품 및 샴푸, 비누 등의 세정제에 적용될 수 있다.The cosmetic composition of the present invention includes skin, lotion, cream, essence, pack, foundation, color cosmetics, sun cream, two-way cake, face powder, compact, makeup base, skin cover, eye shadow, lipstick, lip gloss, lip fix, eyebrow pencil It can be applied to cosmetics such as , lotion, and detergents such as shampoo and soap.
본 발명의 일 실시예에 따른 화장료 조성물에는 상기 고추(pepper)에서 추출한 추출물 이외에 기능성 첨가물 및 일반적인 화장료 조성물에 포함되는 성분이 추가로 포함될 수 있다. 상기 기능성 첨가물로는 수용성 비타민, 유용성 비타민, 고분자 펩티드, 고분자 다당, 스핑고 지질 및 해초 엑기스로 이루어진 군에서 선택된 성분을 포함할 수 있다.The cosmetic composition according to an embodiment of the present invention may further include functional additives and components included in general cosmetic compositions in addition to the extract extracted from the red pepper. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extract.
본 발명의 화장료 조성물에는 또한, 상기 기능성 첨가물과 더불어 필요에 따라 일반적인 화장료 조성물에 포함되는 성분을 배합해도 된다. 이외에 포함되는 배합 성분으로서는 유지 성분, 보습제, 에몰리엔트제, 계면 활성제, 유기 및 무기 안료, 유기 분체, 자외선 흡수제, 방부제, 살균제, 산화 방지제, 식물 추출물, pH 조정제, 알콜, 색소, 향료, 혈행 촉진제, 냉감제, 제한(制汗)제, 정제수 등을 들 수 있다.In the cosmetic composition of the present invention, a component contained in a general cosmetic composition may be further blended with the functional additive as needed. Other ingredients included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation accelerators, cooling agents, limiting agents, purified water, and the like.
이하, 실시예를 통하여 본 발명을 보다 자세히 설명한다. 다만, 상기 실시예 및 실험예는 본 발명에 대한 예시로 제시되는 것으로, 당업자에게 주지 저명한 기술 또는 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명을 생략할 수 있고, 이에 의해 본 발명이 제한되지는 않는다. 본 발명은 후술하는 특허청구범위의 기재 및 그로부터 해석되는 균등 범주 내에서 다양한 변형 및 응용이 가능하다.Hereinafter, the present invention will be described in more detail through examples. However, the above embodiments and experimental examples are presented as examples of the present invention, and when it is determined that detailed descriptions of well-known techniques or configurations known to those skilled in the art may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted. , and the present invention is not limited thereby. Various modifications and applications of the present invention are possible within the scope of equivalents interpreted therefrom and the description of the claims to be described later.
< 1 > 실험방법< 1 > Experimental method
1.1. 추출방법(Extraction)1.1. Extraction
고추 샘플 3.0g에 0.1% acid 6ml으로 4℃, 차광상태로 24h shaking extraction하였다. 그 후 Centrifuge(4000rpm, 10min, 25℃)하여 상등액을 수득하였다.To 3.0 g of red pepper sample, 6 ml of 0.1% acid was subjected to shaking extraction at 4° C. and light-shielding condition for 24 h. After that, centrifuge (4000rpm, 10min, 25℃) to obtain a supernatant.
1.2. Dansyl chloride derivatization1.2. Dansyl chloride derivatization
용매 별 추출 샘플 상등액 150 ul에 포화 NaHCO3 150 ul를 2 times 넣는다. Dansyl chloride(10 mg/ml in acetone) 200 ul을 순서대로 첨가한다(암실에서 진행). 충분한 vortex 후 70 ℃ water bath에서 1h 반응(차광) 시킨다. 샘플을 식히고 proline solution(100 mg/ml) 200 ul을 첨가 후 동일 조건에서 30 min 반응(차광)시켰다. 아세톤(Acetone)을 모두 날리고 톨루엔(toluene) 300 ul을 넣고 2 min간 vortex 시킨다. Centrifuge(7500 rpm, 5 min, 4℃) 후 상등액 200 ul를 새로운 tube에 취하고, 분리된 톨루엔(toluene) 층은 4℃에 차광 보관한다.Add 150 ul of saturated NaHCO 3 to 150 ul of the extraction sample supernatant for each solvent 2 times. Dansyl chloride (10 mg/ml in acetone) 200 ul is added in order (proceed in the dark). After sufficient vortex, react (shielding) for 1 h in 70 ℃ water bath. After cooling the sample, 200 ul of proline solution (100 mg/ml) was added and reacted (shielding) for 30 min under the same conditions. Blow off all acetone, add 300 ul of toluene, and vortex for 2 min. After centrifuge (7500 rpm, 5 min, 4 ℃), 200 ul of the supernatant is taken into a new tube, and the separated toluene layer is stored at 4 ℃ to block light.
1.3. TLC1.3. TLC
TLC plate를 세로 7 cm로 cutting 한다(가로 size의 경우 sample의 수에 따라서 cutting 진행). TLC plate 3 mm안에 2 mm의 간격으로 sample 및 standard를 2 내지 3 ul loading한다. Cyclohexane : Ethyl acetate = 3 : 2로 전개용매를 제조한다. TLC running 후 365 nm에서 검출한다.Cut the TLC plate to 7 cm in length (in the case of horizontal size, cutting proceeds according to the number of samples). 2 to 3 ul of samples and standards are loaded into 3 mm of TLC plate at intervals of 2 mm. Cyclohexane : Ethyl acetate = 3: 2 to prepare a developing solvent. After TLC running, it is detected at 365 nm.
1.4. RP-HPLC1.4. RP-HPLC
질소(Nitrogen)으로 톨루엔(toluene)을 모두 날리고 MeOH 1ml으로 녹인다. 시린지 필터(syringe Filter, 45 um)로 filtration한다. 이동상 : Water (A), MeOH (B)로, Flow rate : 0.7 ml/min, Injection volume : 20 ul, Temperature : 25 ℃, Detector : 254 nm의 조건으로 실시하고 결과는 하기 표 1에 나타내었다.Blow all toluene out with nitrogen and dissolve in 1 ml of MeOH. Filtrate with a syringe filter (45 um). Mobile phase: Water (A), MeOH (B), Flow rate: 0.7 ml/min, Injection volume: 20 ul, Temperature: 25 °C, Detector: 254 nm conditions were carried out, and the results are shown in Table 1 below.
1.5. NO production assay1.5. NO production assay
RAW264.7 cell을 24 well plate에 5 x 104 cells로 seeding한다. 24h 후 고추 추출물을 농도 별(control, x100, x50, x25)로 처리한다. 1 h 후 LPS를 1 ug/ml 농도로 처리한다. 24 h 후 상등액 100 ul을 96 well plate로 옮긴다. Griess A/B solution을 1:1으로 혼합 후 100 ul씩 분주한다. 15 min간 차광 상태에서 반응시킨 후 540 nm에서 흡광도를 측정한다.RAW264.7 cells are seeded at 5 x 10 4 cells in a 24 well plate. After 24 h, the red pepper extract is treated by concentration (control, x100, x50, x25). After 1 h, LPS is treated at a concentration of 1 ug/ml. After 24 h, 100 ul of the supernatant is transferred to a 96 well plate. Mix Griess A/B solution 1:1 and then dispense 100 ul each. After reacting in a light-shielded state for 15 min, absorbance is measured at 540 nm.
1.6. MTT assay1.6. MTT assay
RAW264.7 cell을 5 x 104개로 seeding하여 24 h간 incubator에서 배양한다. 고추 추출물을 농도별로 처리하고 incubator에서 24 h 배양한다. Media 1 ml 중 600 ul를 걷어내고 MTT 시약을 40 ul 처리한다. Incubator에서 3 h간 반응시킨다. Media를 모두 걷어내고 DMSO 700 ul를 분주한다. 96 well plate에 40 ul씩 duplication한다. 550 nm의 파장에서 흡광도를 측정한다.RAW264.7 cells are seeded in 5 x 10 4 pieces and incubated in an incubator for 24 h. The red pepper extract is treated by concentration and incubated for 24 h in an incubator. Remove 600 ul of Media 1 ml and treat 40 ul of MTT reagent. Incubate for 3 h in an incubator. Remove all media and dispense 700 ul of DMSO. Duplicate 40 ul each in a 96 well plate. The absorbance is measured at a wavelength of 550 nm.
1.7. qRT-PCR1.7. qRT-PCR
RAW264.7 cell을 1.5 x 105개로 60 mm dish에 seeding하여 24 h간 배양한다. 고추 추출물을 농도별로 처리하고 incubator에서 24 h 배양한다. Media를 제거 후 cold PBS 2 ml으로 wash, 1ml 분주 후 cell harvest 한다. 원심분리로 상등액을 제거 후 cell pellet을 얻는다. Tri-zol method를 이용해 cell lysis하여 total RNA를 얻는다. cDNA synthesis kit(TOYOBO)와 qRT-PCR kit(TOYOBO)를 이용해 qRT-PCR을 진행한다. Reference gene은 mouse gapdh으로, target gene은 mouse TNF-a, IL-1b, IL-6로 하며 해당 primer를 조성대로 제조하여 진행한다.RAW264.7 cells were seeded in a 60 mm dish with 1.5 x 10 5 cells and cultured for 24 h. The red pepper extract is treated by concentration and incubated for 24 h in an incubator. After removing the media, wash with 2 ml of cold PBS, dispense 1 ml, and harvest the cells. After removing the supernatant by centrifugation, a cell pellet is obtained. Total RNA is obtained by cell lysis using the Tri-zol method. Perform qRT-PCR using cDNA synthesis kit (TOYOBO) and qRT-PCR kit (TOYOBO). The reference gene is mouse gapdh, and the target gene is mouse TNF-a, IL-1b, and IL-6, and the primers are prepared according to the composition and proceeded.
< 2 > 실험결과< 2 > Experimental result
2.1. 용매 별 polyamine 함량 비교2.1. Comparison of polyamine content by solvent
도 1에서 나타낸 것과 같이 0.1 % HCl 용매 사용시 다른 용매 대비 폴리아민 중 스퍼미딘(spermidine) 및 스퍼민(spermine)이 다량 함유되어 있는 것을 확인하였고, 총 폴리마민이 가장 많이 함유되어 있는 것을 확인하였다.As shown in FIG. 1, when 0.1% HCl solvent was used, it was confirmed that spermidine and spermine were contained in large amounts of polyamines compared to other solvents, and it was confirmed that total polymamine was contained the most.
2.2. 용매 별 산화질소(Nitric oxide, NO) production 저해 활성과 cell viability 평가2.2. Evaluation of nitric oxide (NO) production inhibitory activity and cell viability by solvent
도 2A 및 도 2B에서 나타낸 것과 같이 0.1 % HCl 용매 조건에서 다른 용매 대비 NO production이 현저하게 감소됨을 확인할 수 있었고, 이는 약 50 %의 NO production이 감소된 수치이다(도 2A 참조). 또한, NO production을 현저하게 감소시킴과 동시에 cell viability 또한 가장 높은 수준으로 유지하고 있음을 확인하였다(도 2B 참조).As shown in FIGS. 2A and 2B, it was confirmed that NO production was significantly reduced compared to other solvents in the 0.1% HCl solvent condition, which is a reduced value of about 50% NO production (see FIG. 2A). In addition, it was confirmed that while significantly reducing NO production, cell viability was also maintained at the highest level (see FIG. 2B ).
2.3. 0.1% HCl extract의 농도 별 산화질소(Nitric oxide, NO) production 저해 활성과 cell viability 평가2.3. Nitric oxide (NO) production inhibitory activity and cell viability evaluation by concentration of 0.1% HCl extract
도 3A 및 도 3B에서 나타낸 것과 같이 농도 별로 0.1% HCl extract의 NO production 저해 활성에 대해 측정하였으며, 농도 의존적인 NO production 감소를 보임을 확인할 수 있었다. 이때 양성대조군(Positive control, P.C)으로 NIL 10 uM이 사용되었으며 가장 고농도 처리 군에서 양성대조군과 유사한 NO production 감소를 확인할 수 있었다(도 3A 참조). 또한 고농도로 처리한 샘플군에서도 cell viability는 관찰되지 않았다(도 3B 참조).As shown in FIGS. 3A and 3B , the NO production inhibitory activity of 0.1% HCl extract was measured for each concentration, and it was confirmed that a concentration-dependent decrease in NO production was observed. At this time, 10 uM of NIL was used as a positive control (P.C), and a decrease in NO production similar to that of the positive control group was confirmed in the highest concentration treatment group (see FIG. 3A). In addition, cell viability was not observed even in the sample group treated with a high concentration (see FIG. 3B).
2.4. 염증성 cytokines mRNA 발현 변화량 측정2.4. Measurement of changes in mRNA expression of inflammatory cytokines
도 4A, 도 4B 및 도 4C에서 나타낸 것과 같이 LPS 처리로 인해 증가된 염증성 사이토카인인 TNF-a, IL-1b 및 IL-6는 0.1% HCl 고추 추출물을 처리한 경우 현저하게 감소하는 것을 확인하였다. As shown in Figures 4A, 4B and 4C, inflammatory cytokines TNF-a, IL-1b and IL-6 increased by LPS treatment were significantly reduced when treated with 0.1% HCl pepper extract. .
따라서, 상기 실험들에 의해 여러 용매 중 0.1% HCl으로 고추를 추출 시 다량의 폴리아민(polyamine)을 함유하는 것을 확인했다. 또한, 해당 용매로 추출한 extract를 macrophage인 RAW264.7 cell에 처리 후 NO production 생성에 대해 cell viability 영향없이 효과적으로 저해 활성을 나타냄을 확인하였다. 마지막으로 염증성 cytokine 발현 변화는 TNF-a에서 소폭 감소하는 것이 관찰되며, IL-1b와 IL-6에서 농도 의존적으로 감소하는 것을 확인하여 위 결과를 바탕으로 의약품 및 피부 미용 제품의 원료로 0.1% HCl 고추 추출물이 유용하게 사용될 수 있음을 확인하여 본 발명을 완성하였다.Therefore, it was confirmed by the above experiments to contain a large amount of polyamine (polyamine) when extracting red pepper with 0.1% HCl in various solvents. In addition, it was confirmed that the extract extracted with the solvent effectively exhibited inhibitory activity without affecting cell viability on NO production after treatment on RAW264.7 cells, which are macrophages. Finally, the inflammatory cytokine expression change was observed to be slightly decreased in TNF-a, and it was confirmed that the concentration-dependent decrease in IL-1b and IL-6 was confirmed. Based on the above results, 0.1% HCl as a raw material for pharmaceuticals and skin care products The present invention was completed by confirming that the red pepper extract can be usefully used.
이제까지 본 발명에 대하여 그 바람직한 실시 예들을 중심으로 살펴보았다.So far, the present invention has been looked at with respect to preferred embodiments thereof.
본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시 예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구 범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.Those of ordinary skill in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.
Claims (8)
상기 추출물은 산(acid)를 이용하여 추출한 것을 특징으로 하는, 염증 예방 또는 개선용 건강기능식품 조성물.According to claim 1,
The extract is a health functional food composition for preventing or improving inflammation, characterized in that it is extracted using an acid.
상기 산(acid)는 염산(HCl), 과염소산(Perchloric acid), 인산(Phosphoric acid), 아세트산(Acetic acid), 시트르산(Citric acid) 및 폼산(Formic acid) 중 어느 하나인 것을 특징으로 하는, 염증 예방 또는 개선용 건강기능식품 조성물.3. The method of claim 2,
The acid (acid) is hydrochloric acid (HCl), perchloric acid (Perchloric acid), phosphoric acid (Phosphoric acid), acetic acid (Acetic acid), citric acid (Citric acid) and formic acid (Formic acid) characterized in that any one, inflammation, Health functional food composition for prevention or improvement.
상기 추출물은 푸트레신(putrescine), 스퍼미딘(spermidine) 및 스퍼민(spermine)을 함유하고 있는 것을 특징으로 하는, 염증 예방 또는 개선용 건강기능식품 조성물.According to claim 1,
The extract is a health functional food composition for preventing or improving inflammation, characterized in that it contains putrescine, spermidine and spermine.
상기 조성물은 산화질소(NO) 저해 활성을 가지는 것을 특징으로 하는, 염증 예방 또는 개선용 건강기능식품 조성물.According to claim 1,
The composition is a health functional food composition for preventing or improving inflammation, characterized in that it has nitric oxide (NO) inhibitory activity.
상기 조성물은 염증성 사이토카인 TNF-a, IL-1b 및 IL-6의 발현을 억제시키는 것을 특징으로 하는, 염증 예방 또는 개선용 건강기능식품 조성물.According to claim 1,
The composition is a health functional food composition for preventing or improving inflammation, characterized in that it suppresses the expression of inflammatory cytokines TNF-a, IL-1b and IL-6.
상기 1단계 후 4 ℃ 및 차광상태로 24 시간동안 추출하는 2단계;
상기 3단계 후 원심분리기를 통해 상등액을 수득하는 3단계를 포함하는 항염증 활성을 갖는 폴리아민 고함유 고추 추출물의 유효 추출 방법.1 step of treating pepper with any one of hydrochloric acid (HCl), perchloric acid, phosphoric acid, acetic acid, citric acid, and formic acid;
a second step of extracting for 24 hours at 4° C. and in a light-shielded state after the first step;
An effective extraction method of red pepper extract containing a high polyamine having anti-inflammatory activity, comprising the 3 step of obtaining a supernatant through a centrifuge after the 3 step.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160018599A (en) | 2013-06-05 | 2016-02-17 | 콘티넨탈 테베스 아게 운트 코. 오하게 | Method for controlling an electromechanical actuator, and control device |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160018599A (en) | 2013-06-05 | 2016-02-17 | 콘티넨탈 테베스 아게 운트 코. 오하게 | Method for controlling an electromechanical actuator, and control device |
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