KR20220059606A - A Composition for Prevention of Hair Loss and Promotion of Hair Growth Comprising Fermented Bur Reed - Google Patents
A Composition for Prevention of Hair Loss and Promotion of Hair Growth Comprising Fermented Bur Reed Download PDFInfo
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- KR20220059606A KR20220059606A KR1020200144942A KR20200144942A KR20220059606A KR 20220059606 A KR20220059606 A KR 20220059606A KR 1020200144942 A KR1020200144942 A KR 1020200144942A KR 20200144942 A KR20200144942 A KR 20200144942A KR 20220059606 A KR20220059606 A KR 20220059606A
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- South Korea
- Prior art keywords
- black ginseng
- fermented
- hair
- composition
- hair growth
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Abstract
Description
흑삼릉 발효물을 포함하는 탈모방지 및 발모촉진용 조성물에 관한 것이다.It relates to a composition for preventing hair loss and promoting hair growth comprising a fermented product of black ginseng.
모발은 피부 표면에서 생산된 가늘고 각질화된 구조로, 외부 충격에 대한 쿠션 역할과 더불어 직사광선, 한냉, 마찰, 위험 등 외부의 자극으로부터 인체를 보호하고, 신체에 유해한 비소, 수은, 아연 등의 중금속을 체외로 배출하는 기능을 담당한다. 현대에 와서는 외모를 꾸미는데 관여되는 미용측면의 기능도 하고 있다. Hair is a thin, keratinized structure produced on the surface of the skin that not only serves as a cushion against external impact, but also protects the human body from external stimuli such as direct sunlight, cold, friction, and danger, and removes heavy metals such as arsenic, mercury and zinc that are harmful to the body. Responsible for excretion from the body. In modern times, it is also functioning as a cosmetic aspect involved in decorating the appearance.
그러나 식생활 변화나 스트레스의 증가를 비롯해 여러 원인에 의해 탈모를 호소하는 사람들이 증가하여 탈모산업의 시장은 급성장하고 있는 추세이다. However, as the number of people complaining of hair loss due to various causes, including changes in diet or increase in stress, is increasing, the market for hair loss industry is rapidly growing.
사람의 모발은 약 10만 내지 15만개 정도이며, 각각의 모발은 서로 다른 성장주기를 가진다. 모발의 성장 주기는 모발이 가장 활발하게 성장하는 성장기(anagen stage), 모발의 퇴화가 시작되는 퇴화기(catagen stage), 및 모발의 성장이 멈추거나 휴식에 접어드는 휴지기(telogen stage)의 3단계로 구성되어 있다. Human hair is about 100,000 to 150,000, and each hair has a different growth cycle. The hair growth cycle is divided into three stages: the anagen stage, in which hair grows most actively, the catagen stage, in which hair degeneration begins, and the telogen stage, in which hair growth stops or enters rest. Consists of.
이러한 과정의 시작은 진피로 이루어진 모유두세포(hair dermal papilla cell)가 모기질 세포를 자극하여 이루어진다. 모유두세포에서 분비되는 것으로 밝혀진 인슐린 유사 성장 인자(insulin like growth factor(IGF-1)), 간세포 성장 인자(hepatocyte growth factor(HGF)), 혈관 성장 인자(vascular endotherial growth factor(VEGF))등의 성장인자와 성장기에 그 양이 증가하였다가 퇴행기에 감소함으로써 세포의 이동과 분화에 관여하는 모유두세포 기질 등이 모발의 성장을 촉진시키는 것으로 생각되고 있다. 원형탈모증에서 모유두세포를 전자 현미경으로 관찰한 보고에 의하면 형태와 핵이 변형되고, 세포질내에 색소성 과립이나 공포등이 형성되는데 이러한 변화는 모장의 변화가 관찰되기 이전에 이미 발생하므로 이 질환은 모유두세포의 변화로부터 시작된다고 할 수 있다 (Folia Pharmacol. Jpn. (Nippon Yakurigaku Zasshi) 119 (3), pp.167-174 (2002)). The start of this process is made by stimulation of matrix cells by hair dermal papilla cells made up of the dermis. Growth of insulin like growth factor (IGF-1), hepatocyte growth factor (HGF), vascular endotherial growth factor (VEGF), etc. found to be secreted by dermal papilla cells It is thought that the dermal papilla cell matrix, which is involved in cell migration and differentiation, promotes hair growth by increasing the amount of factors and the growth phase and then decreasing it in the catagen phase. According to the report of observing dermal papilla cells in alopecia areata with an electron microscope, the shape and nucleus are transformed, and pigmented granules or vacuoles are formed in the cytoplasm. It can be said that it begins with changes in cells (Folia Pharmacol. Jpn. (Nippon Yakurigaku Zasshi) 119 (3), pp.167-174 (2002)).
현재 미국 식품의약품안전청(FDA)으로부터 승인을 받은 대표적인 탈모치료제로는 합성의약품으로 도포용 미녹시딜과 경구용 프로페시아가 있는데, 이들은 몇 가지 부작용들이 지속적으로 보고되고 있다. 프로페시아는 남성호르몬 (testosterone)에서 탈모의 원인물질인 dihydrotestosterone(DHT)으로 변환되는 과정을 억제하여 효과를 나타내지만 남성의 성욕을 감소시킬 수 있다는 문제가 제기되고 있으며 미녹시딜은 고혈압 치료제로 개발되다가 모낭 활성을 통한 모발 성장 촉진이 보고되면서 양모제로 개발되었지만 다양한 피부질환 등의 부작용이 보고되고 있다.Currently, as representative hair loss treatment agents approved by the US Food and Drug Administration (FDA), there are minoxidil for application and Propecia for oral use as synthetic drugs, and some side effects are continuously reported. Propecia is effective by inhibiting the conversion of male hormone (testosterone) to dihydrotestosterone (DHT), which is the causative agent of hair loss. Although it has been developed as a hair growth agent as it has been reported to promote hair growth through
일 양상은 흑삼릉 발효물을 포함하는 탈모방지 또는 발모촉진용 식품 조성물을 제공한다.One aspect provides a food composition for preventing hair loss or promoting hair growth comprising a fermented product of black ginseng.
다른 양상은 흑삼릉 발효물을 포함하는 탈모방지 또는 발모촉진용 약학적 조성물을 제공한다. Another aspect provides a pharmaceutical composition for preventing hair loss or promoting hair growth comprising a fermented product of black ginseng.
또 다른 양상은 흑삼릉 발효물을 포함하는 탈모방지 또는 발모촉진용 화장료 조성물을 제공한다.Another aspect provides a cosmetic composition for preventing hair loss or promoting hair growth comprising a fermented product of black ginseng.
또 다른 양상은 흑삼릉을 열수추출하는 단계; 상기 열수추출물을 락토바실러스 파라카세이(Lactobacillus paracasei)로 발효하는 단계; 를 포함하는 흑삼릉 발효물 제조 방법을 제공한다. Another aspect is the steps of hot water extraction of black ginseng tombs; Fermenting the hot water extract with Lactobacillus paracasei ; It provides a method for producing a fermented black ginseng tomb comprising a.
일 양상은 흑삼릉 발효물을 포함하는 탈모방지 또는 발모촉진용 식품 조성물을 제공한다. One aspect provides a food composition for preventing hair loss or promoting hair growth comprising a fermented product of black ginseng.
상기 흑삼릉은 Sparganium 에 속하는 식물일 수 있으며, 예를 들면 Sparganium stoloniferum, Sparganium erectum, Sparganium acaule, Sparganium americanum, Sparganium androcladum, Sparganium angustifolium, Sparganium confertum, Sparganium emersum , Sparganium Х englerianum, Sparganium erectum, Sparganium eurycarpum, Sparganium fallax, Sparganium fluctuans, Sparganium glomeratum, Sparganium gramineum, Sparganium hyperboreum, Sparganium japonicum, Sparganium kawakamii, Sparganium limosum, Sparganium Х longifolium, Sparganium natans, Sparganium Х oligocarpon, Sparganium probatovae, Sparganium rothertii, Sparganium Х speirocephalum, Sparganium Х splendens, Sparganium subglobosum, Sparganium yunnanense 일 수 있다.The black ginseng may be a plant belonging to Sparganium , for example , Sparganium stoloniferum, Sparganium erectum, Sparganium acule, Sparganium americanum, Sparganium androcladum, Sparganium angustifolium, Sparganium confertum, Sparganium emersum , Sparganium parganium erectum, Sparganium Х eurganeng eng , Sparganium fluctuans, Sparganium glomeratum, Sparganium gramineum, Sparganium hyperboreum, Sparganium japonicum, Sparganium kawakamii, Sparganium limosum, Sparganium Х longifolium, Sparganium natans, Sparganium Х oligocarpon, Sparganium probatovae, Sparganium rothertii, Sparganium Х speirocephalum, Sparganium Х splendens, Sparganium subglobosum, It can be Sparganium yunnanense .
일 구체예에 따르면, 상기 흑삼릉은 Sparganium stoloniferum 일 수 있다.According to one embodiment, the black ginseng tomb may be Sparganium stoloniferum .
일 구체예에 따르면, 상기 흑삼릉 발효물은 흑삼릉 또는 이의 추출물을 발효한 것일 수 있다. 상기 흑삼릉 추출물은 흑삼릉을 물 또는 탄소수 1내지 4의 알코올, 및 이들의 혼합용매로 구성되는 군으로부터 선택되는 용매로 추출한 추출물 일 수 있으며, 물을 용매로 추출한 추출물일 수 있다.According to one embodiment, the fermented product of black ginseng tomb may be fermented black ginseng or an extract thereof. The black ginseng reung extract may be an extract obtained by extracting black ginseng reung with a solvent selected from the group consisting of water or an alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof, and may be an extract obtained by extracting water with a solvent.
상기 흑삼릉 또는 이의 추출물은 흑삼릉의 잎, 뿌리, 줄기 또는 식물 전체를 사용한 것일 수 있으며, 이를 건조하여 사용한 것일 수 있으나 이에 제한되는 것은 아니다. The black ginseng reung or an extract thereof may be obtained by using the leaves, roots, stems or whole plants of black ginseng reung, and may be dried and used, but is not limited thereto.
일 구체예에 따르면 상기 흑삼릉 또는 이의 추출물은 흑삼릉의 줄기를 사용한 것일 수 있다.According to one embodiment, the black ginseng reung or its extract may be one using the stem of black ginseng reung.
일 구체예에 따르면, 상기 흑삼릉 추출물은 가온 조건에서 추출한 것일 수 있으며 50 내지 100℃ 조건에서 추출한 것일 수 있다.According to one embodiment, the black ginseng extract may be extracted under a heating condition, or may be extracted under a condition of 50 to 100 ℃.
상기 흑삼릉 발효물은 유산균을 통해 발효한 것일 수 있으며, 예를 들면, 락토바실러스 속(Lactobacillus sp.) 균주에 의해 발효되는 것일 수 있고, 락토바실러스 파라카세이(Lactobacillus paracasei)에 의해 발효되는 것일 수 있다.The black ginseng fermented product may be fermented through lactic acid bacteria, for example, may be fermented by a Lactobacillus sp. strain, and may be fermented by Lactobacillus paracasei . .
일 구체예에 따르면, 상기 흑삼릉 발효물은 락토바실러스 파라카세이(Lactobacillus paracasei) HY7015로 발효한 것 일 수 있다. According to one embodiment, the black ginseng fermented product may be fermented with Lactobacillus paracasei HY7015.
상기 락토바실러스 파라카세이(Lactobacillus paracasei) HY7015는 흑삼릉 또는 흑삼릉 추출물 중량 대비 0.01 내지 1.00 중량%로 첨가한 것일 수 있으며, 예를들면, 0.01 내지 0.3 중량% 또는 0.01 내지 1.00 중량%로 첨가한 것일 수 있다.The Lactobacillus paracasei ( Lactobacillus paracasei ) HY7015 may be added in an amount of 0.01 to 1.00% by weight based on the weight of the black ginseng or black ginseng extract, for example, 0.01 to 0.3% by weight or 0.01 to 1.00% by weight. .
상기 흑삼릉 발효물은 발효균을 포함할 수 있으며, 발효 후 배양액, 상층액 또는 배양액을 동결건조한 분말 등을 의미하는 것일 수 있다.The black ginseng fermented product may include fermented bacteria, and may mean a culture solution, a supernatant or a powder obtained by freeze-drying the culture solution after fermentation.
일 구체예에 따르면, 상기 흑삼릉 발효물은 모낭수 증가, 세포 증가 또는 VEGF 발현 촉진 작용이 있는 것을 특징으로 하는 것 일 수 있다. 상기 세포는 모유두세포, 섬유아세포 등 피부와 관련된 세포 일 수 있다. According to one embodiment, the black ginseng fermented product may be one characterized in that it has an effect of increasing the number of hair follicles, increasing cells, or promoting VEGF expression. The cells may be skin-related cells, such as dermal papilla cells and fibroblasts.
본 발명의 조성물이 식품 조성물로 제조되는 경우, 유효성분으로써 흑삼릉 발효물 외에, 식품 제조 시에 통상적으로 첨가되는 성분을 포함할 수 있으며, 예를 들어, 단백질, 탄수화물, 지방, 영양소, 조미제 및 향미제를 포함할 수 있다. 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어, 말토오스, 수크로오스, 올리고당 등; 및 폴리사카라이드, 예를 들어, 덱스트린, 사이클로덱스트린 등과 같은 통상적인 당 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜일 수 있다. 향미제로서 천연 향미제[타우마틴, 스테비아 추출물(예를 들어, 레바우디오시드 A, 글리시르히진 등)] 및 합성 향미제(사카린, 아스파르탐 등)를 사용할 수 있다.When the composition of the present invention is prepared as a food composition, as an active ingredient, in addition to the fermented black ginseng root, ingredients commonly added during food production may be included, for example, proteins, carbohydrates, fats, nutrients, seasonings and Flavoring agents may be included. Examples of carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides, for example, conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, natural flavoring agents [taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)] and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.
예를 들어, 본 발명의 식품 조성물이 드링크제로 제조되는 경우에는 본 발명의 흑삼릉 발효물 외에 구연산, 액상과당, 설탕, 포도당, 초산, 사과산, 과즙, 두충 추출액, 대추 추출액 및/또는 감초 추출액 등이 추가로 포함될 수 있다.For example, when the food composition of the present invention is prepared as a drink, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, licorice root extract, jujube extract and/or licorice extract, etc. may additionally be included.
또한, 본 발명의 식품 조성물은 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. In addition, the food composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and thickeners (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like.
이러한 성분은 독립적으로 또는 조합하여 사용할 수 있으며, 이러한 첨가제의 비율은 본 발명의 식품 조성물 100 중량부 당 0 내지 약 20 중량부의 범위에서 선택될 수 있으나, 이에 한정되는 것은 아니다.These components may be used independently or in combination, and the ratio of these additives may be selected from 0 to about 20 parts by weight per 100 parts by weight of the food composition of the present invention, but is not limited thereto.
일 구체예에 따르면 상기 식품조성물은 건강기능식품, 기능성 음료, 야채주스, 발효유일 수 있으나 이에 제한되지 않는다. According to one embodiment, the food composition may be a health functional food, a functional beverage, vegetable juice, or fermented milk, but is not limited thereto.
일 구체예에 따르면, 상기 식품조성물은 총 조성물 1kg 당 10 내지 500mg의 흑삼릉 발효물을 포함하는 것일 수 있으며, 예를 들면 50 내지 300mg을 포함하는 것일 수 있으나, 이에 제한되는 것은 아니다.According to one embodiment, the food composition may include 10 to 500 mg of fermented black ginseng per 1 kg of total composition, for example, may include 50 to 300 mg, but is not limited thereto.
다른 양상은 흑삼릉 발효물을 포함하는 탈모방지 또는 발모촉진용 약학적 조성물을 제공한다. Another aspect provides a pharmaceutical composition for preventing hair loss or promoting hair growth comprising a fermented product of black ginseng.
상기 흑삼릉, 흑삼릉 추출물, 흑삼릉 발효물, 탈모방지 또는 발모촉진에 대해서는 상기에서 설명한 바와 같으며, 이와 관련된 유산균, 락토바실러스 파라카세이, 및 세포에 대해서도 상기에서 설명한 바와 같다.The black ginseng, black ginseng extract, black ginseng fermented product, hair loss prevention or hair growth promotion is as described above, and related lactic acid bacteria, Lactobacillus paracasei, and cells are also the same as described above.
일 구체예에 따르면, 상기 약학적 조성물은 경구투여제, 경피투여제 또는 외용제로 사용될 수 있다. According to one embodiment, the pharmaceutical composition may be used for oral administration, transdermal administration, or external administration.
상기 약학 조성물은 각각 통상의 방법에 따라 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽, 에어로졸, 액제 등의 경구형 제형 또는 멸균 주사용액의 형태로 제형화하여 사용할 수 있다. 상세하게는 제형화 할 경우 통상 사용하는 충진제, 중량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제될 수 있다. 경구투여를 위한 고형 제제로는 정제, 환제, 산제, 과립제, 캡슐제 등을 포함하나, 이에 한정되는 것은 아니다. 이러한 고형 제제는 상기 흑삼릉 발효물을 포함하는 조성물 또는 이의 약학적으로 허용 가능한 염에 적어도 하나 이상의 부형제, 예를 들면 전분, 칼슘 카보네이트, 수크로오스, 락토오스, 젤라틴 등을 섞어 조제될 수 있다. 비경구 투여를 위한 제제는 멸균된 수용액, 비수성 용제, 현탁제, 유제, 동결건조 제제 및 과제를 포함한다. 비수성 용제 및 현탁제로는 프로필렌글리콜, 폴리에틸렌글리콜, 올리브 오일과 같은 식물성 오일, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다. 좌제의 기제로는 위텝솔, 마크로솔, 트윈 61, 카카오지, 라우린지, 글리세로젤라틴 등을 사용할 수 있다. 본 발명의 조성물의 적합한 투여량은 환자의 상태 및 체중, 질병의 정도, 약물형태, 시간에 따라 다르지만, 당업자에 의해 적절하게 선택될 수 있다.The pharmaceutical composition may be formulated in the form of oral dosage forms or sterile injection solutions such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, and liquids, respectively, according to conventional methods. In detail, when formulating, it can be prepared using a diluent or excipient such as a filler, a weight agent, a binder, a wetting agent, a disintegrant, and a surfactant commonly used. Solid preparations for oral administration include, but are not limited to, tablets, pills, powders, granules, capsules, and the like. Such a solid preparation may be prepared by mixing the composition comprising the fermented black ginseng fermented product or a pharmaceutically acceptable salt thereof with at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, gelatin, and the like. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations and tasks. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used. As the base of the suppository, Witepsol, Macrosol, Tween 61, cacao butter, laurin fat, glycerogelatin, etc. can be used. A suitable dosage of the composition of the present invention varies depending on the patient's condition and weight, the severity of the disease, drug form, and time, but may be appropriately selected by those skilled in the art.
또 다른 양상은 흑삼릉 발효물을 포함하는 탈모방지 또는 발모촉진용 화장료 조성물을 제공한다.Another aspect provides a cosmetic composition for preventing hair loss or promoting hair growth comprising a fermented product of black ginseng.
상기 흑삼릉, 흑삼릉 추출물, 흑삼릉 발효물, 탈모방지 또는 발모촉진에 대해서는 상기에서 설명한 바와 같으며, 이와 관련된 유산균, 락토바실러스 파라카세이, 및 세포에 대해서도 상기에서 설명한 바와 같다.The black ginseng, black ginseng extract, black ginseng fermented product, hair loss prevention or hair growth promotion is as described above, and related lactic acid bacteria, Lactobacillus paracasei, and cells are also the same as described above.
상기 화장료 조성물은 용액, 외용연고, 크림, 폼, 영양화장수, 유연화장수, 팩, 유연수, 유액, 메이크업베이스, 에센스, 비누, 액체 세정료, 입욕제, 선 스크린크림, 선오일, 현탁액, 유탁액, 페이스트, 겔, 로션, 파우더, 비누, 계면활성제-함유 클린싱, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 샴푸, 린스, 컨디셔너, 패취 및 스프레이로 구성된 군으로부터 선택되는 제형으로 제조할 수 있으나, 이에 제한되는 것은 아니다.The cosmetic composition is a solution, external ointment, cream, foam, nutrient lotion, softening lotion, pack, soft water, emulsion, makeup base, essence, soap, liquid detergent, bath agent, sunscreen cream, sun oil, suspension, emulsion, Paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, shampoo, rinse, conditioner, patch and spray, However, the present invention is not limited thereto.
상기 조성물은 모발케어 조성물을 포함하는 것일 수 있다. The composition may include a hair care composition.
본 발명의 화장료 조성물에 포함되는 화장품학적으로 허용 가능한 담체는 제형에 따라 다양하다. 본 발명의 제형이 연고, 페이스트, 크림 또는 젤인 경우에는, 담체성분으로서 동물성 유, 식물성 유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크, 산화아연 또는 이들의 혼합물이 이용될 수 있다.The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation. When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures thereof may be used.
또 다른 양상은 흑삼릉을 열수추출하는 단계; 및 상기 열수추출물을 락토바실러스 파라카세이(Lactobacillus paracasei)로 발효하는 단계;를 포함하는 흑삼릉 발효물 제조 방법을 제공한다.Another aspect is the steps of hot water extraction of black ginseng tombs; and fermenting the hot water extract with Lactobacillus paracasei .
상기 열수추출하는 단계는 흑삼릉을 물 또는 탄소수 1내지 4의 알코올, 및 이들의 혼합용매로 구성되는 군으로부터 선택되는 용매로 추출한 추출물을 발효한 것일 수 있으며, 예를 들면 물을 용매로 추출한 추출물을 발효한 것일 수 있다. The hot water extraction step may be a fermented extract extracted from black ginseng neung with a solvent selected from the group consisting of water or an alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof, for example, an extract obtained by extracting water with a solvent. It may be fermented.
상기 락토바실러스 파라카세이(Lactobacillus paracasei)는 락토바실러스 파라카세이(Lactobacillus paracasei) HY7015 일 수 있다. The Lactobacillus paracasei ( Lactobacillus paracasei ) may be Lactobacillus paracasei ( Lactobacillus paracasei ) HY7015 .
상기 락토바실러스 파라카세이(Lactobacillus paracasei) HY7015는 흑삼릉 또는 흑삼릉 열수추출물 중량 대비 0.01 내지 1.00 중량%로 첨가한 것일 수 있으며, 예를 들면 0.01 내지 0.3 중량% 또는 0.01 내지 1.00 중량%로 첨가한 것일 수 있다.The Lactobacillus paracasei HY7015 may be added in an amount of 0.01 to 1.00% by weight, for example, 0.01 to 0.3% by weight or 0.01 to 1.00% by weight, based on the weight of black ginseng or Heuksamreung hot water extract. .
상기 흑삼릉 발효물은 락토바실러스 파라카세이(Lactobacillus paracasei)를 10시간 내지 30시간동안 배양한 것일 수 있으며, 예를 들면 10 시간 내지 25시간 또는 15시간 내지 25시간일 수 있다. The black ginseng fermented product may be Lactobacillus paracasei cultured for 10 to 30 hours, for example, 10 to 25 hours or 15 to 25 hours.
본 발명에 따른 흑삼릉 발효물을 포함하는 조성물은 탈모 방지 및 발모촉진 효과가 있다. 따라서 상기 조성물은 탈모방지 및 발모촉진을 위한 식품조성물, 약학적 조성물, 화장료 조성물 및 두피 모발케어 조성물로 유용하게 적용할 수 있다.The composition comprising the fermented black ginseng root according to the present invention has the effect of preventing hair loss and promoting hair growth. Therefore, the composition can be usefully applied as a food composition, a pharmaceutical composition, a cosmetic composition, and a scalp hair care composition for preventing hair loss and promoting hair growth.
도 1은 일 구체예에 따른 모유두세포의 증식 효과를 나타내는 데이터이다.
도 2는 일 구체예에 따른 섬유아세포의 증식효과를 나타내는 데이터이다.
도 3은 일 구체예에 따른 혈관신생인자인 VEGF 분비량 유도효과를 나타내는 데이터이다.
도 4는 일 구체예에 따른 제모 마우스 모델에서 모발 촉진 효과를 확인한 사진이다.
도 5는 일 구체예에 따른 제모 마우스 모델에서 모낭수와 모발의 성장을 확인한 조직학적 분석 결과이다.
도 6은 일 구체예에 따른 제모 마우스 모델에서 모낭수를 수치로 나타낸 그래프이다.
도 7은 일 구체예에 따른 제모 마우스 모델에서 진피층 두께를 나타낸 그래프이다.
도 8은 일 구체예에 따른 제모 마우스 모델에서 혈청 내 VEGF를 측정한 결과를 나타낸 그래프이다. 1 is data showing the proliferation effect of dermal papilla cells according to one embodiment.
2 is data showing the proliferation effect of fibroblasts according to an embodiment.
3 is data showing the effect of inducing secretion of VEGF, which is an angiogenic factor, according to an embodiment.
4 is a photograph confirming the hair promotion effect in the hair removal mouse model according to an embodiment.
5 is a histological analysis result confirming the number of hair follicles and hair growth in a hair removal mouse model according to an embodiment.
6 is a graph showing the number of hair follicles numerically in a mouse model for hair removal according to an embodiment.
7 is a graph showing the thickness of the dermal layer in the hair removal mouse model according to an embodiment.
8 is a graph showing the results of measuring VEGF in the serum in the hair removal mouse model according to one embodiment.
이하 하나 이상의 구체예를 실시예를 통해 보다 상세하게 설명한다. 그러나, 이들 실시예는 하나 이상의 구체예를 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다. Hereinafter, one or more specific embodiments will be described in more detail through examples. However, these examples are for illustrative purposes of one or more embodiments, and the scope of the present invention is not limited to these examples.
실시예 1: 흑삼릉 열수추출물의 제조Example 1: Preparation of hot water extract of black ginseng neung
건조된 흑삼릉(Sparganium stoloniferum) 줄기 100g을 분쇄하고 추출용기에 넣은 후, 상기 건조된 흑삼릉 100g을 증류수 1,000mL와 함께 80℃에서 6시간 이상 환류 및 교반 추출하였다. 100 g of dried Sparganium stoloniferum stems were crushed and placed in an extraction container, and 100 g of dried black ginseng reung was refluxed with 1,000 mL of distilled water at 80° C. for more than 6 hours and extracted with stirring.
상기 흑삼릉 열수추출물을 20mesh의 1차 진동체 여과 후 2차 퍼라이트(perlite)로 흡착 및 여과하여 불용성 불순물을 제거하였다. 그런 다음, 상기 여과된 흑삼릉 열수추출물을 회전 진공 농축기(EYELA, Tokyo, Japan)를 사용하여 60℃에서 감압농축하고, 상기 감압 농축된 흑삼릉 열수추출물을 5 브릭스(Brix0)가 되도록 희석하였다.The black ginseng hot water extract was filtered through a 20 mesh primary vibrating sieve and then adsorbed and filtered with secondary perlite to remove insoluble impurities. Then, the filtered black ginseng hot water extract was concentrated under reduced pressure at 60 ° C using a rotary vacuum concentrator (EYELA, Tokyo, Japan), and the concentrated black ginseng hot water extract was diluted to 5 Brix (Brix 0 ).
실시예 2:Example 2: 흑삼릉 발효물의 제조Production of fermented black ginseng tombs
상기 실시예 1의 흑삼릉 열수추출물에 락토바실러스 파라카제이(Lactobacillus paracasei) HY7015 유산균을 추출물의 0.01% 및 1%로 첨가하여 37℃에서 16시간 배양하였다. 배양된 흑삼릉 발효물은 -80℃에서 동결건조(Operon, Gimpo-si, Korea)한 후 분말화하였다. Lactobacillus paracasei HY7015 lactic acid bacteria were added to the hot water extract of black ginseng neung of Example 1 at 0.01% and 1% of the extract, and cultured at 37° C. for 16 hours. The cultured black ginseng fermented product was freeze-dried (Operon, Gimpo-si, Korea) at -80℃ and then powdered.
상기의 방법을 통해 락토바실러스 파라카제이(Lactobacillus paracasei) HY7015 유산균을 0.01% 첨가하여 발효한 흑삼릉 발효물 및 락토바실러스 파라카제이(Lactobacillus paracasei) HY7015 유산균을 1% 첨가하여 발효한 흑삼릉 발효물을 제조하였다. Through the above method, Lactobacillus paracasei HY7015 lactic acid bacteria were added to 0.01% of black ginseng fermented product and Lactobacillus paracasei HY7015 lactic acid bacteria were added to 1% of fermented black ginseng fermented product. did.
실시예 3: 모유두세포의 증식 효과 확인Example 3: Confirmation of the proliferation effect of dermal papilla cells
모근을 구성하는 모유두세포의 증식 효과를 확인하기 위하여, 모유두세포를 24 웰 플레이트(well plate)에 5.0x103 cells/well로 배양한 뒤, 상기 실시예 2의 흑삼릉 발효물을 25μg/mL, 50μg/mL 농도로 처리한 후에 24시간 및 48시간 동안 배양하였다. 배양 후, 인산완충식염수로 2회 세척하여 MTT assay를 진행하였다. 양성대조군(Serum)은 10% 소태아혈청(Fetal Bovine Serum, FBS)를 첨가하였으며 음성대조군(Con) 및 흑삼릉 발효물 군에는 2% 농도의 소태아혈청 (FBS)을 첨가하여 진행하였다. In order to confirm the proliferation effect of the dermal papilla cells constituting the hair root, after culturing the dermal papilla cells at 5.0x10 3 cells/well in a 24-well plate, 25 μg/mL, 50 μg of the black ginseng fermented product of Example 2 After treatment with /mL concentration, incubated for 24 hours and 48 hours. After incubation, MTT assay was performed by washing twice with phosphate buffered saline. For the positive control group (Serum), 10% Fetal Bovine Serum (FBS) was added, and to the negative control group (Con) and the fermented Heuksamneung group, 2% fetal bovine serum (FBS) was added.
그 결과 도 1과 같이, 상기 실시예 흑삼릉 발효물을 처리한 군들에서 모유두세포가 대조군 대비 증식된 것을 확인하였다.As a result, as shown in FIG. 1, it was confirmed that the dermal papilla cells were proliferated compared to the control group in the group treated with the black ginseng fermented product of Example.
모유두세포는 모낭을 구성하는 세포이며 모발을 성장시키는 모모세포를 유지하는 세포므로, 흑삼릉 발효물을 처리한 군들에서 모유두 세포가 증식되었다는 것은 모발 성장에 효과가 있음을 의미한다. Since dermal papilla cells are cells constituting hair follicles and are cells that maintain hair cells that grow hair, the proliferation of dermal papilla cells in the group treated with black ginseng fermented product means that it is effective for hair growth.
실시예 4: 섬유아세포의 증식 효과 확인Example 4: Confirmation of the proliferation effect of fibroblasts
동물의 결합조직인 섬유아세포(Fibroblat)의 증식 효과를 확인하기 위하여 NIH3T3 섬유아세포를 24 웰 플레이트(well plate)에 5.0x103cells/well로 배양한 뒤, 상기 실시예 2의 흑삼릉 발효물을 25μg/mL, 50μg/mL 농도로 처리한 후에 24시간 및 48시간 배양하였다. 배양 후, 인산완충식염수로 2회 세척하여 MTT assay를 진행하였다. 양성대조군(Serum)은 10% 소태아혈청(Fetal Bovine Serum, FBS)를 첨가하였으며 음성대조군(Con) 및 흑삼릉 발효물 군에는 2% 농도의 소태아혈청 (FBS)을 첨가하여 진행하였다. In order to confirm the proliferation effect of fibroblasts, which are connective tissues of animals, NIH3T3 fibroblasts were cultured in a 24-well plate at 5.0x10 3 cells/well, and 25 μg/ mL, and incubated for 24 hours and 48 hours after treatment at a concentration of 50 μg/mL. After incubation, MTT assay was performed by washing twice with phosphate buffered saline. For the positive control group (Serum), 10% Fetal Bovine Serum (FBS) was added, and to the negative control group (Con) and the fermented Heuksamneung group, 2% fetal bovine serum (FBS) was added.
그 결과 도 2와 같이, 상기 실시예 흑삼릉 발효물을 처리한 군들에서 섬유아세포 증식이 대조군 대비 증가한 것을 확인할 수 있었으며, 특히 락토바실러스 파라카제이(Lactobacillus paracasei) HY7015 유산균을 1% 첨가하여 발효한 흑삼릉 발효물에서 더 증가한 것을 확인할 수 있었다.As a result, as shown in FIG. 2, it was confirmed that fibroblast proliferation was increased in the groups treated with the black ginseng fermented product of the above example compared to the control group, and in particular, Lactobacillus paracasei HY7015 lactic acid bacteria were added to 1% of the fermented black ginseng tombs. It was confirmed that the fermented product further increased.
섬유아세포는 피부에서 모낭을 구성하는 인자 중 하나로, 섬유아세포 증식 효과는 흑삼릉 발효물이 세포 성장 및 유지에 기여한다는 것을 의미한다. Fibroblasts are one of the factors constituting hair follicles in the skin, and the fibroblast proliferation effect means that the fermented black ginseng root contributes to cell growth and maintenance.
실시예 5: 모유두세포 VEGF(vascular endothelial growth factor) 단백질 분비 향상 효과 확인Example 5: Confirmation of the effect of improving dermal papilla cell vascular endothelial growth factor (VEGF) protein secretion
모근을 구성하는 모유두세포의 모발 성장 촉진 효과를 확인하기 위하여, 모발 성장에 도움이 된다고 알려진 단백질인 VEGF의 분비량을 확인하였다. 모유두세포를 24 웰 플레이트에 5x103 cells/well로 배양한 뒤, 상기 실시예 2의 흑삼릉 발효물을 25μg/mL 농도로 처리한 후에 48시간 동안 배양하였다. 그런 다음, 배양이 끝난 상층액에서 Human VEGF ELISA Kit(DVE00, R&D systems, USA)를 이용하여 상층액 내 VEGF 분비량을 측정하였다. 양성대조군(Serum)은 10% 소태아혈청(Fetal Bovine Serum, FBS)를 첨가하였으며 음성대조군(무처리군) 및 흑삼릉 발효물 군에는 2% 농도의 소태아혈청 (FBS)을 첨가하여 진행하였다. In order to confirm the hair growth promoting effect of the dermal papilla cells constituting the hair root, the secretion amount of VEGF, a protein known to help hair growth, was confirmed. After dermal papilla cells were cultured in a 24-well plate at 5x10 3 cells/well, the fermented black ginseng fermented product of Example 2 was treated at a concentration of 25 μg/mL and then cultured for 48 hours. Then, the amount of VEGF secretion in the supernatant was measured using the Human VEGF ELISA Kit (DVE00, R&D systems, USA) from the cultured supernatant. For the positive control group (serum), 10% fetal bovine serum (FBS) was added, and to the negative control group (untreated group) and the fermented black ginseng fermented product group, 2% fetal bovine serum (FBS) was added.
그 결과 도 3과 같이, 1% 흑삼릉 발효물 군에서 음성대조군 대비 혈관신생인자인 VEGF 분비량이 증가한 것을 확인 할 수 있었다. As a result, as shown in FIG. 3, it was confirmed that the secretion of VEGF, an angiogenic factor, increased in the 1% black ginseng fermented product group compared to the negative control group.
VEGF는 혈관신생인자로, VEGF가 증가하는 것은 혈관이 증가하여 혈액순환이 촉진되는 것을 의미한다. 따라서, 상기 결과는 흑삼릉 발효물이 원활한 혈액순환을 통해 모발에 영양소를 공급할 수 있는 효과를 가지고 있다는 것을 의미한다.VEGF is an angiogenic factor, and an increase in VEGF means that blood vessels increase and blood circulation is promoted. Therefore, the above result means that the fermented black ginseng root has an effect of supplying nutrients to the hair through smooth blood circulation.
실시예 6: 발모 실험 모델 마우스에서 모발 성장 효과 확인Example 6: Confirmation of hair growth effect in mouse hair growth test model
제모기를 이용하여 7주령된 C57BL/6 암컷 마우스의 등 부위에 자란 털을 모두 제거하였으며, 털을 제모하는 과정 중 피부에 상처가 난 마우스는 실험에서 제외하여 3마리의 마우스를 사용하여 실험하였다. 제모한 마우스에게 상기 실시예 2의 흑삼릉 발효물을 일반 사료(AIN-93G)에 200 mg/kg 농도로 혼합하여 제공하였다. 7주 동안 제공하였으며 매 주마다 제모한 마우스의 털이 자라는 정도를 관찰하였다. 양성대조군으로는 시중에서 판매되고 있는 탈모방지용 약용효모추출물을 200 mg/kg 농도로 사료에 첨가하여 제공하였다.All hairs growing on the back of 7-week-old C57BL/6 female mice were removed using an epilator, and mice with skin wounds during hair removal were excluded from the experiment and 3 mice were used. The hair-removed mice were mixed with the black ginseng fermented product of Example 2 in a general feed (AIN-93G) at a concentration of 200 mg/kg and provided. It was provided for 7 weeks, and the degree of hair growth of the shaved mice was observed every week. As a positive control group, a commercially available medicinal yeast extract for preventing hair loss was added to the feed at a concentration of 200 mg/kg and provided.
그 결과 도 4와 같이, 무처리군에서는 털이 성장하지 않았던 반면, 양성대조군 및 흑삼릉 발효물을 섭취한 쥐에서는 털이 성장한 결과를 확인할 수 있었다. As a result, as shown in FIG. 4 , while hair did not grow in the untreated group, it was confirmed that hair grew in the positive control group and the mice fed the black ginseng fermented product.
실시예 7: 발모 실험 마우스의 모낭 수 및 피부조직 두께 확인Example 7: Confirmation of the number of hair follicles and the thickness of the skin tissue in the hair growth test mouse
상기 실시예 4 실험 후, 각 처리군의 마우스 등 피부를 수직 및 수평으로 절단한 후, 절단면에 H&E(Hematoxylin & Eosin) 염색을 수행하였다. 수평으로 절단한 피부의 절단면을 통해서 조직의 모구(Hair Follicle)를 계수하였다. 계수한 모구를 피부 조직 면적대비 모낭수로 표현하여 도 6과 같이 나타내었다. 또한, 수직으로 절단한 피부의 절단면을 통해서 피부 조직에서 하얀색으로 표현되는 진피층의 두께를 측정하여 도 7과 같이 그래프로 표현하였다.After the experiment of Example 4, the skin of each treatment group, such as mice, was cut vertically and horizontally, and then H&E (Hematoxylin & Eosin) staining was performed on the cut surface. The hair follicles of the tissue were counted through the cut surface of the skin cut horizontally. The counted hair follicles were expressed as the number of hair follicles compared to the skin tissue area, as shown in FIG. 6 . In addition, the thickness of the dermal layer expressed in white in the skin tissue was measured through the cut surface of the skin cut vertically, and expressed as a graph as shown in FIG. 7 .
상기 실험 결과로 도 5와 같이, 양성대조군 및 발효물을 처리한 쥐의 등 피부에서 모낭수가 증가한 것과 모발이 성장하는 것을 관찰하였다. 또한 도 6과 같이 발효물을 처리한 마우스의 모낭수는 음성군 대비 현저하였으며, 발효물을 처리한 마우스의 모낭수 증가가 양성대조군보다 증가된 것을 확인하였다. 도 7과 같이, 진피층의 두께는 양성대조군 및 발효물 처리한 쥐 모두 증가하는 것을 확인하였다.As a result of the above experiment, as shown in FIG. 5 , an increase in the number of hair follicles and hair growth were observed in the back skin of the mice treated with the positive control group and the fermented product. In addition, as shown in FIG. 6 , the number of hair follicles in the mice treated with the fermented product was significant compared to the negative group, and it was confirmed that the increase in the number of hair follicles in the mice treated with the fermented product was increased compared to the positive control group. As shown in FIG. 7 , it was confirmed that the thickness of the dermal layer increased in both the positive control group and the fermented mice.
실시예 8: 발모 실험 마우스의 혈액 내 VEGF 단백질 분비량 확인Example 8: Confirmation of the amount of VEGF protein secretion in the blood of the hair growth test mice
상기 실시예 5를 실시한 후, 실험한 7주차의 마우스들을 희생시켰다. 그 후, 혈액을 수거하여 상온 방치하므로 혈청을 분리하였다. mouse VEGF ELISA Kit(MMV00, R&D systems, USA)를 통해 혈청 내 분비된 VEGF 단백질량을 측정하였다.After carrying out Example 5, mice at the 7th week of the experiment were sacrificed. After that, blood was collected and left at room temperature, so that serum was separated. The amount of VEGF protein secreted in the serum was measured through the mouse VEGF ELISA Kit (MMV00, R&D systems, USA).
그 결과 도 8과 같이, 음성대조군인 무처리군에 비해 양성대조군과 발효물을 처리한 쥐에서는 VEGF 단백질의 분비가 현저히 증가한 것을 확인하였다. As a result, as shown in FIG. 8, it was confirmed that the secretion of VEGF protein was significantly increased in the mice treated with the positive control group and the fermented product compared to the untreated group, which is the negative control group.
Claims (7)
A food composition for preventing hair loss or promoting hair growth, comprising fermented black ginseng.
The food composition for preventing hair loss or promoting hair growth according to claim 1, wherein the black ginseng fermented product is a fermented hot water extract.
The food composition for preventing hair loss or promoting hair growth according to claim 1, wherein the black ginseng fermented product is fermented with Lactobacillus paracasei HY7015.
The food composition for preventing hair loss or promoting hair growth according to claim 1, wherein the black ginseng fermented product has an effect of increasing the number of hair follicles, increasing cells, or promoting VEGF expression.
A pharmaceutical composition for preventing hair loss or promoting hair growth comprising a fermented product of black ginseng.
A cosmetic composition for preventing hair loss or promoting hair growth, comprising fermented black ginseng.
상기 열수추출물을 락토바실러스 파라카세이(Lactobacillus paracasei)로 발효하는 단계;
를 포함하는 흑삼릉 발효물 제조 방법.
Preparing a hot water extract of black ginseng tomb; and
Fermenting the hot water extract with Lactobacillus paracasei ;
A method for producing fermented black ginseng tombs comprising a.
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