KR20210079100A - Cultivation method of Cordyceps sinensis containing collagen - Google Patents
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Abstract
Description
본 발명은 콜라겐이 함유된 동충하초의 재배방법에 대한 것으로서, 이를 보다 상세히 설명하면 콜라겐이 다량으로 함유된 동충하초를 재배하기 위한 방법을 통하여 보다 향상된 건강증진효과를 제공토록 한 콜라겐이 함유된 동충하초의 재배방법에 관한 것이다.The present invention relates to a method for cultivating Cordyceps cordyceps containing collagen, which is described in more detail. Cultivation of Cordyceps containing collagen to provide a more improved health-promoting effect through a method for cultivating Cordyceps cordyceps containing a large amount of collagen it's about how
일반적으로 동충하초(vegetable worms, 冬蟲夏草)는 겨울에는 곤충의 몸에 있다가 여름에는 풀처럼 나타난다는 데서 비롯되어 이름 지어진 버섯으로서, 넓은 의미로 곤충에 기생하는 모든 종류의 버섯을 일컫는 것이며, 대부분 곤충에 기생하여 숙주가 되는 곤충의 시체에 자실체(子實體)를 내며, 자실체는 머리와 줄기의 두 부분으로 이루어지며, 머리 부분은 공 모양, 양 끝이 뾰쪽한 원기둥 모양, 주걱 모양 등으로 불룩해지며, 그 표면 또는 표피 아래에 여러 개의 자낭각(子囊殼)을 이루는 것이며, 숙주가 되는 곤충은 나비목(붉은동충하초:Cordyceps militaris)·매미목(매미동충하초:C. sobolifera)·벌목(벌동충하초:C. sphecocephala), 그 밖에 딱정벌레목·메뚜기목 외에 거미에게도 기생하는 것이며, 이러한 동충하초의 균은 숙주를 죽이고 곤봉 모양 또는 줄 모양 등의 자실체인 버섯을 내며, 이와 같은 동충하초는 세계적으로 널리 분포되어 300~400여 종이 알려져 있는 실정이며, 이러한 동충하초는 예로부터 인삼, 녹용 등과 같이 귀한 약재로 사용되어 왔으며, 성질이 달고 순하며, 신장과 폐를 보하고, 강장 및 정력 향상과 빈혈 등에 효과가 있어서 삼계탕 등의 요리에 넣어서 몸을 보하는 데도 사용되어 왔으며, 근래에 들어서 면역력 증강 밑 당뇨병 예방 및 치료와 혈압 정상화 및 해독작용, 항암 등에도 효과가 있다는 연구사례가 나오고 있는 실정이었다.In general, vegetable worms (vegetable worms, 冬蟲夏草) are named after the fact that they are in the body of insects in winter and appear like grass in summer. In a broad sense, it refers to all kinds of mushrooms that parasitize insects, and most are parasitic on insects. Thus, a fruiting body is produced on the corpse of an insect that becomes a host, and the fruiting body consists of two parts, the head and the stem, and the head bulges into a ball shape, a cylindrical shape with pointed ends, a spatula shape, etc. It forms several ascomyx shells on its surface or under the epidermis, and the insects that serve as hosts are Lepidoptera (Cordyceps militaris), Hemiptera (C. sobolifera), and Hymenoptera (C. sphecocephala) , and other parasites on spiders in addition to Coleoptera and Locustaceae, the fungus of Cordyceps Cordyceps kills the host and produces club-shaped or string-shaped fruiting bodies such as mushrooms, and such Cordyceps cordyceps is widely distributed around the world, and there are about 300 to 400 species of Cordyceps. It is a known fact, and such cordyceps has been used as precious medicinals such as ginseng and antler since ancient times, and has a sweet and mild nature, nourishes the kidneys and lungs, improves tonicity and energy, and is effective in anemia, etc. It has been used to protect the body, and in recent years, there have been studies showing that it is effective in preventing and treating diabetes, normalizing blood pressure, detoxifying, and anti-cancer under strengthening immunity.
이와 같이 몸에 좋은 동충하초를 자연적으로는 구하기가 어려워서 인공적으로 재배를 하여 대량으로 제공할 수 있는 방법을 모색하고 있으며 일부 인공 재배방법들이 개발되고 있었던 것이며, 근자에는 보다 몸에 좋은 재배방법들이 개발되고 있었던 것으로서, 종래의 동충하초 재배방법으로는 대한민국 공개특허 2013년 제57507호(2013년 6월 3일자 공개)에 게재된 바와 같이 유채박혼합물과 갈파래과녹조류가 함유된 배지에 종균을 접종하고 암(暗)배양으로 발아 및 광(光)배양으로 생육토록 하는 재배방법으로 동충하초를 재배할 수 있도록 한 기술이 개발되어 왔으나. 배지보다는 암배양과 광배양 등의 배양방법에 의해서 말라타리스 동충하초를 재배토록 하였으나, 이러한 종래 배양방법으로만은 동충하초의 다양한 기능성을 향상시키는데 한계가 있었던 것이다.As such, it is difficult to obtain healthy cordyceps naturally, so we are looking for a way to artificially cultivate and provide in large quantities, and some artificial cultivation methods have been developed. Recently, more healthy cultivation methods have been developed. As a conventional method for cultivating Cordyceps, as disclosed in Korean Patent Application Laid-Open No. 57507 (published on June 3, 2013), seed bacteria were inoculated into a medium containing rapeseed gourd mixture and green algae, and cancer (暗). ), a technology has been developed that enables the cultivation of Cordyceps by a cultivation method that allows germination by culturing and growth by light culture. Although Malataris Cordyceps was cultivated by culturing methods such as dark culture and light culture rather than medium, there was a limit in improving various functions of Cordyceps Cordyceps only with these conventional culturing methods.
이 외에도 대한민국 공개특허 2016년 제33911호(2016년 3월 29일자 공개)에 게재된 바와 같이 종균을 접종하여 배양할 때 편백나무 기능수를 가습하면서 재배토록 한 기술이 개발되어 왔으나, 이 역시 동충하초가 가습되는 편백나무 기능수를 흡수하여 인체에 유익한 성분을 가질 수는 있었으나, 가습만으로 동충하초의 뿌리를 통해서 영양분이 흡수되기에 무리가 따랐으며, 공개특허 2019년 제101792호(2019년 9월 2일자 공개)에 게재된 바와 같이 코디세핀의 함량이 증가되도록 증숙과 건조를 반복한 동충하초의 재배방법이 개발되어 있었으나, 이 역시 단순히 증숙과 건조만을 반복하여 특정 성분의 함량이 높아질 뿐, 원하는 기능을 동충하초에 주입토록 하는 기술은 아니었으며, 상기의 다양한 종래 기술들은 통해서 원하는 성분을 동충하초에 부가시킬 수는 없었던 것이다.In addition, as disclosed in Korean Patent Application Laid-Open No. 33911 (published on March 29, 2016), a technology has been developed that allows cultivation of functional water of cypress while humidifying when inoculating and culturing the seed, but this is also Cordyceps Cordyceps. Although it was possible to have beneficial ingredients for the human body by absorbing the humidified cypress water, it was difficult to absorb nutrients through the roots of Cordyceps by only humidification, and Patent Publication No. 101792 (September 2, 2019) As published in the publication date), a cultivation method of Cordyceps Cordyceps by repeating steaming and drying to increase the content of cordycepin has been developed, but this also simply repeats steaming and drying to increase the content of specific components, and to achieve the desired function. It was not a technology to inject into Cordyceps Cordyceps, and it was not possible to add a desired component to Cordyceps through the above various prior techniques.
본 발명에서는 상기한 종래 기술의 제반 문제점들을 해결코자 새로운 기술을 창안한 것으로서, 본 발명은 숙주를 통해서 원하는 영양분을 동충하초로 흡수토록 하는 기술로서, 동충하초의 숙주가 되는 꽃뱅이에게 특정성분이 함유된 먹이를 공급한 후 종균을 접종시켜서 꽃뱅이를 숙주로 하는 동충하초가 꽃뱅이가 먹이로 먹은 특정성분을 오롯이 흡수토록 한 기술을 제공코자 하되, 흡수시키고자 하는 특정성분을 콜라겐으로 하여 꽃뱅이가 동물성 콜라겐을 먹이로 하지 않으므로 식물성 콜라겐이 다량으로 함유된 금화규를 배지에 포함시켜 꽃뱅이의 먹이로 삼아서 동충하초가 숙주인 꽃뱅이가 먹은 금화규에 포함된 콜라겐을 직접적으로 흡수토록 한 콜라겐이 함유된 동충하초의 재배방법을 제공코자 함에 발명에서 해결하고자 하는 과제를 두고 완성한 것이다.In the present invention, a new technology was invented to solve the problems of the prior art. The present invention is a technology for absorbing desired nutrients into Cordyceps Cordyceps through a host. We intend to provide a technology that allows the Cordyceps Cordyceps, which uses the cauliflower as the host, to absorb the specific ingredients eaten by the cauliflower by inoculating the seed bacteria after supplying the food. However, by using the specific ingredient to be absorbed as collagen, the cauliflower is made of animal origin. Collagen is not used as food, so the medium contains Geumhwagyu, which contains a large amount of vegetable collagen, as food for the cauliflower, so that the Cordyceps Cordyceps directly absorbs the collagen contained in the cauliflower eaten by the host. In order to provide a method for cultivation of Cordyceps Cordyceps, it was completed with the task to be solved in the invention.
또한 별도로 기술하지는 않았으나, 본 발명의 콜라겐이 함유된 동충하초의 재배방법을 상세하게 기술한 하기의 발명을 실시하기 위한 구체적인 내용과 청구범위 및 도면 등을 감안하여 유추할 수 있는 범위 내의 또 다른 목적들도 본 발명의 전체 해결과제에 포함되는 것이다.In addition, although not separately described, other objects within the range that can be inferred in consideration of the specific content, claims, drawings, etc. for carrying out the following invention, which describes in detail the method of cultivation of cordyceps containing collagen of the present invention Also included in the overall solution of the present invention.
상기한 발명의 과제를 해결하기 위한 구체적인 수단으로 본 발명에서는 콜라겐이 함유된 동충하초의 재배방법에 있어서, 상기 본 발명의 콜라겐이 함유된 동충하초는 동충하초에 함유되도록 하고자 하는 특정성분이 함유된 필수대상물이 포함되는 먹이로 사육하여 키운 숙주를 급냉시켜서 종균을 접종하여 재배되도록 하되, 상기 숙주의 먹이는 참나무톱밥과, 금화규와, 유용미생물과, 당밀과, 대두박과, 밀기울과, 물이 혼합 조성된 것을 특징으로 한다.As a specific means for solving the problems of the present invention, in the present invention, in the cultivation method of Cordyceps Cordyceps containing collagen, the collagen-containing Cordyceps of the present invention contains a specific component to be contained in Cordyceps Cordyceps. The host bred and raised with the included food is quenched to be inoculated with a seed so that it is grown, but the food of the host is a mixture of oak sawdust, Geumhwagyu, useful microorganisms, molasses, soybean meal, wheat bran, and water. characterized.
이때 숙주 먹이에 포함되는 필수대상물은 특정성분인 콜라겐이 함유된 금화규로 이루어지며, 숙주는 꽃뱅이로 이루어지며, 종균은 밀리타리스균으로 이루어지며, 숙주 먹이의 각 조성물 함량은 참나무톱밥 40~50 중량%와, 금화규 20~30 중량%와, 유용미생물 1~3 중량%와, 당밀 4~6 중량%와, 대두박 5~10 중량%와, 밀기울 5~10 중량%와, 물 4~12 중량%으로 이루어져서 조성됨을 특징으로 한다.At this time, the essential object included in the host feed consists of Geumhwagyu containing collagen, a specific ingredient, the host consists of cauliflower, the spawn consists of Militaris bacteria, and the content of each composition in the host food is 40 oak sawdust. ~50% by weight, 20 to 30% by weight of Keumhwagyu, 1-3% by weight of useful microorganisms, 4 to 6% by weight of molasses, 5 to 10% by weight of soybean meal, 5 to 10% by weight of bran, and 4% by weight of water It is characterized in that it is composed of ~12 wt%.
이러한 본 발명의 콜라겐이 함유된 동충하초의 재배방법은 금화규의 잎을 줄기로부터 따서 20℃의 상온에서 숙성하여 준비토록 하는 금화규준비단계를 구비하고, 상기 금화규준비단계에 의해서 얻어진 금화규 20~30 중량%을 참나무톱밥 40~50 중량%, 유용미생물 1~3 중량%, 당밀 4~6 중량%, 대두박 5~10 중량%, 밀기울 5~10 중량%, 물 4~12 중량%와 함께 혼합하여 발효를 시키되, 5주간 1차 호기발효 후 2차, 3차 발효는 4주 간격으로 수분을 공급해서 배합하여 추가적인 호기발효가 일어나도록 해서 사육도중 발효열의 발생을 최소화시킬 수 있게 해서 숙주의 먹이로 조성토록 하는 먹이조성단계를 구비하며, 상기 먹이조성단계를 거쳐서 조성된 먹이를 사육배지로 하여 65%의 함수량에 맞추어 수분을 조절한 후 10kg을 계량하여 36L의 상자에 넓게 깔아두고 원활한 채란을 위해 사육실의 온도는 28℃로 설정해서 사육배지에 숙주의 성충 60마리를 일주일간 투입하여 성충으로부터 300~400개의 알을 받도록 한 채란단계를 구비하며, 상기 채란단계를 거쳐서 받은 알이 부화되면 20~28℃의 온도와 65%의 습도에서 13주 동안 먹이를 교체하면서 사육하여 1령의 유충을 3령 말기의 유충으로 성장토록 하는 숙주사육단계를 구비하며, 상기 숙주사육단계를 거쳐서 얻어진 완전히 성장된 유충을 급냉시켜서 숙주를 완성하는 숙주완성단계를 구비하며, 콜라겐분말을 희석시켜서 만든 배양물에 종균을 투입하여 균사체로 배양토록 하는 균사체준비단계를 구비하며, 상기 숙주완성단계에서 급냉된 숙주에 균사체준비단계에서 얻어진 균사체를 접종하여 개별통에 100g씩 나누어 넣고 20℃에서 종균이 성장하도록 수분을 공급함과 동시에 광을 조사하는 접종단계를 구비하며, 상기 접종단계를 거쳐서 숙주에 접종된 종균이 자라기 시작하면서 15~20일이 경과 후 재배실의 온도를 18℃로 조정하고, 60~65일까지의 성장기를 경과할 때까지 수분을 분무기를 통하여 투여해서 습도를 65%로 유지하면서 동충하초를 재배토록 하는 동충하초재배단계를 구비되도록 한 것을 특징으로 한다.This method of cultivating Cordyceps Cordyceps containing collagen of the present invention includes a preparation step of preparing Geumhwagyu by picking the leaves of Geumhwagyu from the stem and ripening it at room temperature of 20°C, and 20 Geumhwagyu obtained by the preparing step of Geumhwagyu. ~30% by weight with oak sawdust 40-50% by weight, useful microorganisms 1-3% by weight, molasses 4-6% by weight, soybean meal 5-10% by weight, wheat bran 5-10% by weight, water 4-12% by weight Mix and ferment, but after the first aerobic fermentation for 5 weeks, the second and third fermentations are mixed by supplying moisture at intervals of 4 weeks to allow additional aerobic fermentation to occur, minimizing the generation of fermentation heat during breeding. A feed composition step is provided to make it into feed, and the feed prepared through the feed composition step is used as a breeding medium, moisture is adjusted to a moisture content of 65%, and then weighed 10 kg and spread widely in a 36 L box. For this purpose, the temperature of the breeding room is set to 28 ℃, and 60 adult insects of the host are put in the breeding medium for a week to receive 300 to 400 eggs from the adults, and the eggs received through the hatching step are hatched. A host breeding step is provided in which the 1st instar larvae grow into the late 3rd instar larvae by rearing them while changing food for 13 weeks at a temperature of 20 to 28°C and a humidity of 65%, and the completely obtained through the host breeding step is provided. A host completion step of rapidly cooling the grown larvae to complete a host, a mycelium preparation step of culturing a mycelium by introducing a seed into a culture made by diluting collagen powder, and the host quenched in the host completion step The mycelium obtained in the mycelium preparation step is inoculated into each container, divided by 100 g into individual containers, and an inoculation step of irradiating light while supplying moisture so that the spawn can grow at 20 ° C., the seed inoculated into the host through the inoculation step After 15 to 20 days have elapsed from the start of growth, the temperature of the cultivation room is adjusted to 18°C, and moisture is administered through a sprayer until the growing season of 60 to 65 days has elapsed to grow Cordyceps while maintaining the humidity at 65%. sat It is characterized in that it is equipped with a cordyceps cultivation step to lock.
이때 상기 채란단계 및 숙주사육단계에서 숙주는 꽃뱅이가 선택 공급되며, 상기 균사체준비단계에서 종균은 밀리타리스균이 선택 공급되며, 상기 동충하초재배단계에서 분무기로 분무되는 수분은 수소수가 선택 공급되도록 할 수 있다.At this time, in the chaeran step and host breeding step, cauliflower is selectively supplied to the host, in the mycelium preparation step, Militaris is selectively supplied as the seed, and the water sprayed with a sprayer in the Cordyceps cultivation step is hydrogen water selectively supplied. can do.
이에 더하여 상기 숙주사육단계는 상기 채란단계를 거쳐서 받은 알을 28℃에서 8~10일 사이 부화된 1령의 유충을 28℃의 온도와 65%의 습도에서 먹이의 호기발효가 이루어지도록 하면서 5주간 사육하여 2령 말 내지 3령 초의 평균 1.8g의 유충으로 성장토록 하는 숙주1차사육단계와, 상기 숙주1차사육단계를 거쳐서 성장한 유충을 먹이를 갈고 20℃의 온도와 65%의 습도에서 먹이의 호기발효가 이루어지도록 하면서 4주간 사육하여 3령 3g의 유충으로 성장토록 하는 숙주2차사육단계와, 상기 숙주2차사육단계를 거쳐서 성장한 유충을 먹이를 갈고 20℃의 온도와 65%의 습도에서 다시 먹이의 호기발효가 이루어지도록 하면서 4주간 사육하여 3령 말기 3.5g의 유충으로 성장토록 하는 숙주3차사육단계로 분할되게 이루어진 것을 특징으로 한다.In addition, the host breeding step is carried out with the first instar larvae hatched between 8 and 10 days at 28°C of the eggs received through the egg-laying step for 5 weeks while allowing aerobic fermentation of the food at a temperature of 28°C and a humidity of 65%. The host primary breeding step to grow into larvae of an average of 1.8 g at the end of the 2nd instar to the beginning of the 3rd instar, and the larvae grown through the host primary breeding step are ground and fed at a temperature of 20°C and a humidity of 65%. The host secondary breeding step to grow into larvae of 3 instar 3g by breeding for 4 weeks while allowing the aerobic fermentation to take place, and grinding the larvae grown through the host secondary breeding step to feed the larvae at a temperature of 20°C and a humidity of 65% It is characterized in that it is divided into the host tertiary breeding stage, which is reared for 4 weeks while allowing the aerobic fermentation of the food to occur again in the larvae to grow into 3.5 g larvae at the end of the third instar.
상술한 과제 해결을 위한 구체적인 수단에 의하면, 본 발명의 콜라겐이 함유된 동충하초의 재배방법은 원하는 특정성분인 콜라겐이 함유된 식물인 금화규를 동충하초의 숙주인 꽃뱅이의 먹이로 사용하여 숙주의 먹이 활동에 의해서 자연스럽게 특정성분이 숙주의 몸속으로 섭취되어 유입되도록 하고, 숙주에 종균을 접종하여 동충하초를 재배할 시 숙주가 섭취한 특정성분을 동충하초가 오롯이 흡수하게 되므로 해서 동충하초가 콜라겐을 다량으로 자연스럽게 흡수할 수 있도록 하는 효과를 갖는 것으로서, 이러한 동충하초의 재배방법에 의하면 특정성분인 콜라겐을 보다 손쉽게 숙주를 통해서 동충하초로 공급토록 하여 동충하초가 콜라겐을 다량으로 함유토록 하는 효과를 가지는 것이다.According to the specific means for solving the above-mentioned problems, the cultivation method of Cordyceps Cordyceps containing collagen of the present invention uses Geumhwagyu, a plant containing collagen, which is a desired specific component, as food for cauliflower, the host of Cordyceps, and feeds the host. Cordyceps naturally absorbs a large amount of collagen by allowing specific ingredients to be naturally ingested and introduced into the host's body through activity, and by inoculating the host to grow Cordyceps by inoculating the host to absorb the specific ingredients ingested by the host. According to the cultivation method of Cordyceps Cordyceps, a specific component, collagen, is more easily supplied to Cordyceps through the host, and thus Cordyceps Cordyceps contains a large amount of collagen.
이러한 콜라겐이 함유된 동충하초를 사람이 섭취하게 되면 당연히 동충하초 본연의 효능과 더불어서 이러한 동충하초에 함유된 콜라겐의 효능도 동시에 갖도록 하는 효과를 가지는 것이다.When a person consumes Cordyceps Cordyceps containing such collagen, of course, it has the effect of simultaneously having the efficacy of the collagen contained in Cordyceps Cordyceps as well as the original efficacy of Cordyceps Cordyceps.
이에 의하면 본 발명에서는 금화규를 숙주인 꽃뱅이의 먹이로 주기만 하는 수고로움만으로 콜라겐을 함유한 동충하초를 재배할 수 있으므로 재배방법이 극히 용이하므로 재배 시의 편의성을 제공하면서 작업자의 수고로움과 피로함을 현저히 극감시킬 수 있는 등 다수의 효과를 가지는 것이다.According to this, in the present invention, since cordyceps containing collagen can be cultivated with only the labor of giving Geumhwagyu as food for the host cauliflower, the cultivation method is extremely easy, thus providing convenience during cultivation and reducing the labor and fatigue of workers. It has a number of effects, such as being able to significantly reduce it.
그리고 콜라겐이 함유된 금화규를 먹이로 제공하여 사육한 꽃뱅이를 숙주로 함과 더불어서 콜라겐분말을 희석시켜서 만든 배양물이 내입된 배지에 종균(=밀리타리스균)을 투입하여 배양된 균사체를 상기 금화규를 먹여 사육한 숙주(=굼벵이)에 접종하도록 함을 특징으로 하며, 이로 인하여 숙주가 섭취한 콜라겐성분 외에도 동충하초의 종균이 배양될 시에도 콜라겐분말이 혼합된 배양물에 의해서 콜라겐이 섭취되어 추후 동충하초가 콜라겐 성분을 한층 더 내포하고 있도록 할 수 있는 효과를 가지는 것이다.In addition, the mycelium cultured by introducing the seed bacteria (= Militaris bacteria) into the medium containing the culture made by diluting the collagen powder as the host is the cauliflower bred by providing Geumhwagyu containing collagen as food. It is characterized in that the host (= slugs) bred by feeding the Geumhwagyu is inoculated. Due to this, in addition to the collagen component ingested by the host, collagen is ingested by the culture in which the collagen powder is mixed even when the seeds of Cordyceps cordyceps are cultured. This will have the effect of allowing the cordyceps to contain more collagen components in the future.
또한 콜라겐을 숙주를 통해서 자연스럽게 동충하초에 흡수되도록 함으로써 별도의 장치가 더 필요하지 않고, 재배방법이 어렵지 않으므로 동충하초의 재배가 극히 용이하면서 재배에 필요한 비용을 절감시킬 수 있는 등 그 기대되는 효과가 다대한 발명인 것이다.In addition, since collagen is naturally absorbed into Cordyceps through the host, no additional equipment is required, and the cultivation method is not difficult, so the cultivation of Cordyceps is extremely easy and the cost required for cultivation can be reduced. it is an invention
도 1은 본 발명의 동충하초의 제조방법을 순차적으로 보인 공정도1 is a process chart sequentially showing the method for producing Cordyceps Cordyceps according to the present invention;
본 발명은 콜라겐이 다량으로 함유된 동충하초를 재배하기 위한 방법을 통하여 보다 향상된 건강증진효과를 제공토록 한 콜라겐이 함유된 동충하초와 이의 재배방법을 제공코자 하는 것으로서, 이를 하기에서 도면들과 함께 보다 구체적으로 설명토록 하되, 첨부된 도면은 본 발명의 기술적 사상의 내용과 범위를 쉽게 설명하기 위한 예시일 뿐 이에 한정되는 것은 아니며, 사용되는 용어들 역시 실시 예를 구체적으로 설명하기 위한 것일 뿐 해당 용어에 국한되게 해석되어서는 아니 된다.The present invention is to provide a cordyceps cordyceps containing collagen and a method for cultivating the same to provide a more improved health-promoting effect through a method for cultivating cordyceps containing a large amount of collagen, which will be described in more detail with the drawings below. However, the accompanying drawings are only examples for easily explaining the content and scope of the technical idea of the present invention, and are not limited thereto, and the terms used are also only for describing the embodiment in detail. It should not be construed as being limited.
상기 본 발명의 콜라겐이 함유된 동충하초는 동충하초에 함유되도록 하고자 하는 특정성분을 콜라겐으로 하여 이러한 콜라겐이 함유된 필수대상물을 금화규로 해서 상기 금화규가 포함되는 먹이를 사용하여 숙주를 사육하고, 이와 같이 키운 숙주를 급냉시킨 후 동충하초의 종균을 접종하여 동충하초를 재배토록 하되, 상기 숙주로는 일명 꽃뱅이라고 불리는 흰점박이꽃무지를 사용하며, 상기 종균으로는 밀리타리스(Cordyceps militaris)균으로 선택하여 사용토록 한다.In the Cordyceps Cordyceps containing collagen of the present invention, a specific component to be contained in Cordyceps Cordyceps is used as collagen, and the essential object containing this collagen is Gold Hwagyu, and the host is reared using the food containing Keumhwagyu, and this After the host grown together is quenched, inoculated with a seed of Cordyceps Cordyceps to grow Cordyceps. As the host, a white-spotted daisy called cauliflower is used, and as the seed, Militaris (Cordyceps militaris) is selected. to use it.
상기 흰점박이꽃무지는 꽃무지족에 속한 대형 풍뎅이로서, 약간 편평하며 딱딱한 인상을 주는 풍뎅이의 일종인데 공중에서 잘 날기 때문에 눈에 잘 띄며, 앞날개를 펼치지 않고 나는데 그 육중한 소리는 말벌과 같으며, 점박이꽃무지와 자주 헷갈리는 것으로 알려져 있으며, 꽃에 모여들기도 하고, 나무의 수액에 모여들기도 한다. 몸길이는 17~22mm이면서 체색은 구릿빛 검은색이며 광택이 있으며, 앞가슴등판과 딱지날개에는 불규칙한 흰색 내지 황백색 무늬들이 있고 곰보처럼 파인 홈이 많다. 이러한 흰점박이꽃무지의 애벌레를 굼벵이 또는 꽃뱅이라고도 하며, 약재로 많이 사용되며, 성충은 늦봄부터 가을까지 볼 수 있으며, 한반도 전역에서 볼 수 있다.The white-spotted flower beetle is a large beetle belonging to the flower dwarf family. It is a kind of beetle that gives a slightly flat and hard impression. It is conspicuous because it flies well in the air, and it flies without spreading its forewings. , is known to be often confused with spotted flower igneous, and it gathers in flowers and sometimes in the sap of trees. The body length is 17~22mm, the body color is coppery black, it is glossy, and there are irregular white or yellowish white patterns on the pronotum and elytra, and there are many grooves like gombo. The larvae of this white-spotted flower beetle are also called slugs or flower moths, and are widely used medicinally. Adults can be seen from late spring to autumn, and can be seen all over the Korean Peninsula.
이러한 본 발명은 동충하초에 콜라겐이 함유되도록 하기 위한 것으로서, 우선 본 발명의 동충하초를 재배할 때 사용되는 숙주를 꽃뱅이로 하되, 이러한 꽃뱅이의 먹이를 준비하여 사육토록 해야 하는바, 상기 숙주의 먹이로는 참나무톱밥과, 금화규와, 유용미생물과, 당밀과, 대두박과, 밀기울과, 물이 혼합 조성토록 하여 사육 시 호기발효가 이루어지도록 한다.The present invention is intended to contain collagen in Cordyceps Cordyceps. First, the host used for cultivating Cordyceps Cordyceps of the present invention is a cauliflower. In the furnace, aerobic fermentation takes place during breeding by mixing oak sawdust, gold hwagyu, useful microorganisms, molasses, soybean meal, bran, and water.
상기 숙주의 먹이를 이루는 조성물의 각 함량을 상세히 기술하면, 참나무톱밥 40~50 중량%와, 금화규 20~30 중량%와, 유용미생물 1~3 중량%와, 당밀 4~6 중량%와, 대두박 5~10 중량%와, 밀기울 5~10 중량%와, 물 4~12 중량%이 각각 혼합 조성되도록 하는 것이다.If each content of the composition constituting the host's food is described in detail, 40-50% by weight of oak sawdust, 20-30% by weight of Geumhwagyu, 1-3% by weight of useful microorganisms, and 4-6% by weight of molasses, 5 to 10% by weight of soybean meal, 5 to 10% by weight of wheat bran, and 4 to 12% by weight of water are mixed to form a composition, respectively.
본 발명은 상기와 같이 조성된 먹이를 숙주에 먹이면서 사육하고, 사육된 숙주를 이후 급냉시킨 다음, 숙주에 밀라타리스균을 종균으로 하여 접종하여 재배가 이루어지도록 하여 콜라겐이 함유된 동충하초의 재배방법을 구현하되, 이를 도 1에 도시된 바와 같이 순차적으로 나열된 공정도를 참조로 하여 하기와 같이 보다 상세히 설명토록 한다.The present invention breeds the food prepared as described above while feeding the host, and then quenches the bred host, and then inoculates the host with Milataris as a seed to cultivate it, thereby cultivating the collagen-containing cordyceps. The method is implemented, but it will be described in more detail as follows with reference to the sequentially listed process diagram as shown in FIG. 1 .
1. 금화규준비단계1. Geumhwagyu preparation stage
금화규의 잎을 줄기로부터 따서 20℃의 상온에서 숙성하여 준비토록 한다.The leaves of Geumhwagyu are picked from the stems and ripened at room temperature at 20℃ to prepare them.
이때 금화규는 미세하게 분쇄하여 하기의 먹이조성단계에 투입하며, 금화규의 잎 외에도 금화규 꽃도 사용할 수 있는 것으로서, 이는 금화규의 각부 중 식물성 콜라겐이 많이 함유된 부위를 사용할 수 있는 것이다.At this time, Keumhwagyu is finely pulverized and put into the food preparation step below, and in addition to the leaves of Keumhwagyu, Keumhwagyu flowers can also be used, which means that parts containing a lot of vegetable collagen among the parts of Keumhwagyu can be used.
2. 먹이조성단계2. Food composition stage
상기 금화규준비단계에 의해서 얻어진 금화규 20~30 중량%을 참나무톱밥 40~50 중량%, 유용미생물 1~3 중량%, 당밀 4~6 중량%, 대두박 5~10 중량%, 밀기울 5~10 중량%, 물 4~12 중량%와 함께 혼합하여 발효를 시키되, 5주간 1차 호기발효 후 2차, 3차 발효는 4주 간격으로 수분을 공급해서 배합하여 추가적인 호기발효가 일어나도록 해서 사육도중 발효열의 발생을 최소화시킬 수 있게 해서 숙주의 먹이로 조성토록 한다.20 to 30% by weight of goldfish obtained in the above preparation step of goldfish, 40-50% by weight of oak sawdust, 1-3% by weight of useful microorganisms, 4-6% by weight of molasses, 5-10% by weight of soybean meal, 5-10% by weight of wheat bran Fermentation is carried out by mixing it with wt% and 4 to 12 wt% of water, but after the first aerobic fermentation for 5 weeks, the second and third fermentations are mixed with moisture at intervals of 4 weeks to allow additional aerobic fermentation to occur during breeding. It should be made as food for the host by minimizing the generation of fermentation heat.
3. 채란단계3. Chaeran stage
상기 먹이조성단계를 거쳐서 조성된 먹이를 사육배지로 하여 65%의 함수량에 맞추어 수분을 조절한 후 10kg을 계량하여 36L의 상자에 넓게 깔아두고 원활한 채란을 위해 사육실의 온도는 28℃로 설정해서 사육배지에 숙주의 성충 60마리를 일주일간 투입하여 성충으로부터 300~400개의 알을 받도록 한다.Using the feed prepared through the feed composition step as a breeding medium, moisture is adjusted to a moisture content of 65%, weighing 10 kg, spread it out in a box of 36 L, and setting the temperature in the breeding room to 28 ° C for smooth egg production Inject 60 adult worms of the host into the medium for a week to receive 300-400 eggs from the adults.
4. 숙주사육단계4. Host breeding stage
상기 채란단계를 거쳐서 받은 알이 부화되면 20~28℃의 온도와 65%의 습도에서 13주 동안 먹이를 교체하면서 사육하여 1령의 유충을 3령 말기의 유충으로 성장토록 한다.When the eggs received through the chaebolization step are hatched, the first instar larvae are grown to the third instar larvae by changing the food for 13 weeks at a temperature of 20 to 28 ° C and a humidity of 65%.
이러한 숙주사육단계는 하기와 같이 1차 5주간, 2~3차에 각각 4주간씩 나누어서 먹이를 교체하면서 숙주인 꽃뱅이의 무게를 측정하도록 하였다.In this host breeding stage, the weight of the host, the cauliflower, was measured while replacing the feed by dividing the first 5 weeks and the second and third weeks for 4 weeks as follows.
(1) 숙주1차사육단계(1) Host primary breeding stage
상기 채란단계를 거쳐서 받은 알을 28℃에서 8~10일 사이 부화된 1령의 유충을 28℃의 온도와 65%의 습도에서 먹이의 호기발효가 이루어지도록 하면서 5주간 사육하여 하기 표에서와 같이 2령 말 내지 3령 초의 평균 1.8g의 유충으로 성장하게 된다.The first-instar larvae, which were hatched between 8 and 10 days at 28 ° C., were bred for 5 weeks while allowing the aerobic fermentation of the food to take place at a temperature of 28 ° C and a humidity of 65%, and as shown in the table below. At the end of the 2nd instar to the beginning of the 3rd instar, they will grow into larvae with an average weight of 1.8g.
(2) 숙주2차사육단계(2) Host secondary breeding stage
상기 숙주1차사육단계를 거쳐서 성장한 유충을 먹이를 갈고 20℃의 온도와 65%의 습도에서 먹이의 호기발효가 이루어지도록 하면서 4주간 사육하여 하기 표에서와 같이 3령 3g의 유충으로 성장하게 된다.The larvae grown through the host primary breeding step are ground to feed and bred for 4 weeks while allowing aerobic fermentation of the food at a temperature of 20 ° C and a humidity of 65% to grow into larvae of 3 instar 3 g as shown in the table below. .
(3) 숙주3차사육단계(3) Host tertiary breeding stage
상기 숙주2차사육단계를 거쳐서 성장한 유충을 먹이를 갈고 20℃의 온도와 65%의 습도에서 다시 먹이의 호기발효가 이루어지도록 하면서 4주간 사육하여 하기 표에서와 같이 3령 말기 3.5g의 유충으로 성장하게 된다.The larvae grown through the host secondary breeding step were ground for food and reared for 4 weeks while allowing the aerobic fermentation of the food to take place again at a temperature of 20°C and a humidity of 65%. As shown in the table below, the larvae at the end of 3rd instar were 3.5g. will grow
이와 같이 사육된 꽃뱅이는 빅깔이 기존의 우윳빛깔에서 노르스름한 빛깔로 변화함을 볼 수 있는 것이다.It can be seen that the big color of the flower buds raised in this way changes from the existing milk color to a yellowish color.
5. 숙주준비단계5. Host preparation stage
상기 13주간의 숙주사육단계를 거쳐서 사육된 꽃뱅이를 매일 세척하되 이틀간 금화규분말을 먹이고 하루 동안 금식시켜서 몸속의 잔여톱밥을 제거하는 한다.The cauliflower bred through the 13-week host breeding stage is washed every day, but the remaining sawdust in the body is removed by feeding the ginseng powder for two days and fasting for one day.
6. 숙주완성단계6. Host Completion Step
상기 숙주사육단계를 거쳐서 얻어진 완전히 성장된 유충을 급냉시켜서 숙주로 사용할 수 있도록 완성한다.The fully grown larvae obtained through the host breeding step are quenched and completed so that they can be used as hosts.
7. 균사체준비단계7. Mycelium preparation step
상기 숙주와는 별도로 콜라겐분말을 희석시켜서 만든 배양물에 종균을 투입하여 균사체로 배양토록 하되, 이와 같이 본 발명은 숙주의 먹이로 콜라겐이 함유된 금화규를 먹이는 방법과 종균에 콜라겐분말이 희석된 배양물을 공급하여 2중으로 콜라겐 성분을 동충하초로 제공토록 하는 것이다.Separately from the host, the seed is added to a culture made by diluting the collagen powder and cultured as a mycelium. As such, the present invention provides a method of feeding Geumhwagyu containing collagen as a feed for the host and a method of feeding the collagen powder to the seed. By supplying the culture, it is to provide the collagen component to the cordyceps cordyceps twice.
8. 접종단계8. Inoculation stage
상기 숙주완성단계에서 급냉된 숙주에 균사체준비단계에서 얻어진 균사체를 접종하여 개별통에 100g씩 나누어 넣고 20℃에서 종균이 성장할 수 있도록 수분(水分)을 공급함과 동시에 광(光)을 조사시킨다.The host quenched in the host completion step is inoculated with the mycelium obtained in the mycelium preparation step, divided into individual containers by 100 g, and water is supplied so that the spawn can grow at 20° C. and light is irradiated at the same time.
9. 동충하초재배단계9. Cordyceps Cultivation Stage
상기 접종단계를 거쳐서 숙주에 접종된 종균이 자라기 시작하면서 15~20일이 경과 후 재배실의 온도를 18℃로 조정하고, 60~65일까지의 성장기를 경과할 때까지 수분을 분무기를 통하여 투여해서 습도를 65%로 유지하면서 동충하초를 재배토록 해서 콜라겐이 함유된 동충하초를 얻을 수 있으며, 이와 같이 콜라겐사료와 콜라겐종균으로 콜라겐배지에서 충분히 공급받은 동충하초는 콜라겐 성분이 풍부하여 코디세핀(cordycepin)의 고단위 기능성식품이 되는 것이다.After 15 to 20 days have elapsed as the seed cells inoculated into the host through the inoculation step begin to grow, the temperature of the cultivation room is adjusted to 18° C., and moisture is administered through a sprayer until the growth period of 60 to 65 days has elapsed. Cordyceps containing collagen can be obtained by cultivating cordyceps while maintaining humidity at 65%. It will become a high-unit functional food.
상기와 같이 본 발명은 식물성 콜라겐이 함유된 금화규를 먹이로 먹여 사육한 숙주인 꽃뱅이을 급냉시켜서 콜라겐분말이 희석된 배양물에서 배양된 종균을 접종시켜 동충하초를 재배토록 함으로써, 동충하초가 콜라겐을 다량으로 자연스럽게 흡수할 수 있도록 하여 콜라겐을 보다 손쉽게 숙주를 통해서 동충하초로 공급토록 해서 동충하초가 콜라겐을 다량으로 함유토록 하는 효과와, 동충하초를 섭취 시 동충하초 본연의 효능과 더불어서 이러한 동충하초에 함유된 콜라겐의 효능도 득할 수 있는 효과와, 금화규를 숙주인 꽃뱅이의 먹이로 주기만 하면 콜라겐을 함유한 동충하초를 재배할 수 있어서 재배방법이 극히 용이하다는 효과와, 숙주에 의한 콜라겐 흡수와 배양 시의 콜라겐 흡수로 동충하초가 콜라겐 성분을 한층 더 내포하도록 하는 효과 및 별도의 장치 없이 콜라겐을 동충하초로 공급하여 재배가 극히 용이하면서 재배 비용이 현저히 절감되는 효과까지도 갖는 등 다양한 효과를 발휘하는 기술인 것이다.As described above, the present invention provides a large amount of Cordyceps by inoculating a seed cultured in a culture diluted with collagen powder to grow Cordyceps by rapid cooling of the host, which is bred by feeding Geumhwagyu containing vegetable collagen as food. In addition to the natural efficacy of Cordyceps Cordyceps when ingested, the effect of the collagen contained in Cordyceps cordyceps is also increased by making it easier to supply collagen through the host to the Cordyceps cordyceps so that it can be absorbed naturally. The effect that can be obtained, the effect that the cultivation method is extremely easy because it is possible to grow Cordyceps containing collagen simply by feeding Geumhwagyu beef as the food to the host, Cordyceps, and the absorption of collagen by the host and collagen during culturing of Cordyceps Cordyceps. It is a technology that exhibits various effects, such as the effect of further embedding collagen components, and the fact that it is extremely easy to cultivate by supplying collagen to Cordyceps without a separate device, and even has the effect of significantly reducing the cultivation cost.
이상과 같이 본 발명의 상세한 설명에는 본 발명의 가장 바람직한 실시 예에 관하여 설명하였으나, 본 발명의 기술범위에 벗어나지 않는 범위 내에서는 다양한 변형실시도 가능하다 할 것이며, 따라서 본 발명의 보호범위는 상기 실시 예에 한정하여 정해지는 것이 아니라, 후술하는 특허청구범위의 기술들과 이들 기술로부터 균등한 기술수단들에까지 보호범위가 인정되어야 할 것이다.As described above, in the detailed description of the present invention, the most preferred embodiment of the present invention has been described, but various modifications may be made within the scope that does not depart from the technical scope of the present invention. It is not limited to the example, but the scope of protection should be recognized from the techniques of the claims to be described later and equivalent technical means from these techniques.
Claims (5)
A host raised with a feed containing an essential object containing a specific ingredient to be contained in Cordyceps cordyceps is quenched and inoculated with a seed so that the host is cultivated, but the feed of the host is oak sawdust, Geumhwagyu, useful microorganisms, and molasses Cordyceps containing collagen, characterized in that it is a mixture of fruits, soybean meal, wheat bran, and water.
숙주 먹이에 포함되는 필수대상물은 특정성분인 콜라겐이 함유된 금화규로 이루어지며;
숙주는 꽃뱅이로 이루어지며;
종균은 밀리타리스균으로 이루어지며;
숙주 먹이의 각 조성물 함량은 참나무톱밥 40~50 중량%와, 금화규 20~30 중량%와, 유용미생물 1~3 중량%와, 당밀 4~6 중량%와, 대두박 5~10 중량%와, 밀기울 5~10 중량%와, 물 4~12 중량%으로 이루어져서 조성됨을 특징으로 하는 콜라겐이 함유된 동충하초.
The method of claim 1 ;
The essential object included in the host food is composed of Keumhwagyu containing collagen as a specific ingredient;
The host consists of cauliflower;
The spawn consists of Militaris;
The content of each composition of the host feed is 40-50 wt% of oak sawdust, 20-30 wt% of Keumhwagyu, 1-3 wt% of useful microorganisms, 4-6 wt% of molasses, 5-10 wt% of soybean meal, Cordyceps containing collagen, characterized in that it is composed of 5 to 10% by weight of bran and 4 to 12% by weight of water.
금화규준비단계에 의해서 얻어진 금화규 20~30 중량%을 참나무톱밥 40~50 중량%, 유용미생물 1~3 중량%, 당밀 4~6 중량%, 대두박 5~10 중량%, 밀기울 5~10 중량%, 물 4~12 중량%와 함께 혼합해서 숙주의 먹이로 조성토록 하는 먹이조성단계;
먹이조성단계를 거쳐서 조성된 먹이를 사육배지로 하여 65%의 함수량에 맞추어 수분을 조절한 후 10kg을 계량하여 36L의 상자에 넓게 깔아두고 원활한 채란을 위해 사육실의 온도는 28℃로 설정해서 사육배지에 숙주의 성충 60마리를 일주일간 투입하여 성충으로부터 300~400개의 알을 받도록 한 채란단계;
채란단계를 거쳐서 받은 알이 부화되면 20~28℃의 온도와 65%의 습도에서 13주 동안 먹이를 교체하면서 사육하여 1령의 유충을 3령 말기의 유충으로 성장토록 하는 숙주사육단계;
숙주사육단계를 거쳐서 얻어진 완전히 성장된 유충을 급냉시켜서 숙주를 완성하는 숙주완성단계;
콜라겐분말을 희석시켜서 만든 배양물에 종균을 투입하여 균사체로 배양토록 하는 균사체준비단계;
숙주완성단계에서 급냉된 숙주에 균사체준비단계에서 얻어진 균사체를 접종하여 개별통에 100g씩 나누어 넣고 20℃에서 종균이 성장하도록 수분을 공급함과 동시에 광을 조사하는 접종단계;
접종단계를 거쳐서 숙주에 접종된 종균이 자라기 시작하면서 15~20일이 경과 후 재배실의 온도를 18℃로 조정하고, 60~65일까지의 성장기를 경과할 때까지 수분을 분무기를 통하여 투여해서 습도를 65%로 유지하면서 동충하초를 재배토록 하는 동충하초재배단계;
가 포함된 것을 특징으로 하는 콜라겐이 함유된 동충하초의 재배방법.
Geumhwagyu preparation step of picking the leaves of the goldfish from the stem and ripening them at room temperature of 20℃;
20~30% by weight of Keumhwagyu obtained in the Keumhwagyu preparation step, 40~50% by weight of oak sawdust, 1~3% by weight of useful microorganisms, 4~6% by weight of molasses, 5~10% by weight of soybean meal, 5~10% by weight of bran %, a food composition step of mixing with 4 to 12% by weight of water to form a food for the host;
Using the feed prepared through the feed composition step as a breeding medium, adjust the moisture to a moisture content of 65%, weigh 10 kg, spread it widely in a 36L box, and set the temperature of the breeding room to 28℃ for smooth egg production. Chaeran step in which 60 adult worms of the host were put into the larvae for a week to receive 300-400 eggs from the adults;
When the eggs received through the incubation step are hatched, the host breeding step is to grow the first instar larvae into the third instar larvae by rearing them while changing the food for 13 weeks at a temperature of 20~28℃ and a humidity of 65%;
A host completion step of rapidly cooling the fully grown larvae obtained through the host breeding step to complete the host;
A mycelium preparation step of introducing a seed to the culture made by diluting the collagen powder and culturing it as a mycelium;
An inoculation step of inoculating the host quenched in the host completion step with the mycelium obtained in the mycelium preparation step, dividing 100 g into each container, supplying moisture to grow the spawn at 20°C, and irradiating light at the same time;
After 15 to 20 days have elapsed as the seed inoculated into the host begins to grow through the inoculation stage, the temperature of the cultivation room is adjusted to 18℃, and moisture is administered through a sprayer until the growth period of 60 to 65 days has elapsed. Cordyceps cultivation step to grow Cordyceps while maintaining the humidity at 65%;
A method of cultivation of Cordyceps Cordyceps containing collagen, characterized in that it contains.
채란단계 및 숙주사육단계에서 숙주는 꽃뱅이가 선택 공급되며;
균사체준비단계에서 종균은 밀리타리스균이 선택 공급되며;
동충하초재배단계에서 분무기로 분무되는 수분은 수소수가 선택 공급됨;
을 특징으로 하는 콜라겐이 함유된 동충하초의 재배방법.
4. The method of claim 3;
In the spawning stage and host breeding stage, the host is selectively supplied with cauliflower;
In the mycelium preparation stage, the seed bacterium is selectively supplied with Militaris;
Hydrogen water is selectively supplied for moisture sprayed with a sprayer in the cordyceps cultivation stage;
A method of cultivation of Cordyceps Cordyceps containing collagen, characterized in that
숙주사육단계는 상기 채란단계를 거쳐서 받은 알을 28℃에서 8~10일 사이 부화된 1령의 유충을 28℃의 온도와 65%의 습도에서 먹이의 호기발효가 이루어지도록 하면서 5주간 사육하여 2령 말 내지 3령 초의 평균 1.8g의 유충으로 성장토록 하는 숙주1차사육단계;
상기 숙주1차사육단계를 거쳐서 성장한 유충을 먹이를 갈고 20℃의 온도와 65%의 습도에서 먹이의 호기발효가 이루어지도록 하면서 4주간 사육하여 3령 3g의 유충으로 성장토록 하는 숙주2차사육단계;
상기 숙주2차사육단계를 거쳐서 성장한 유충을 먹이를 갈고 20℃의 온도와 65%의 습도에서 다시 먹이의 호기발효가 이루어지도록 하면서 4주간 사육하여 3령 말기 3.5g의 유충으로 성장토록 하는 숙주3차사육단계;
로 분할되게 이루어진 것을 특징으로 하는 콜라겐이 함유된 동충하초의 재배방법.4. The method of claim 3;
In the host breeding step, 1st instar larvae hatched between 8-10 days at 28°C of the eggs received through the egg-laying step are bred for 5 weeks while allowing aerobic fermentation of food at a temperature of 28°C and humidity of 65%. Host primary breeding stage to grow into larvae of 1.8 g on average between the end of instar and the beginning of the third instar;
The host second breeding step is to grind the larvae grown through the first host breeding step and breed them for 4 weeks while allowing the aerobic fermentation of the food at a temperature of 20°C and a humidity of 65% to grow into larvae of 3 instar 3g. ;
Host 3, which grows into larvae of 3.5 g at the end of the third instar by grinding the larvae grown through the host secondary breeding step and rearing them for 4 weeks while allowing the aerobic fermentation of the food to take place again at a temperature of 20 ° C and a humidity of 65%. tea breeding stage;
A method of cultivation of Cordyceps Cordyceps containing collagen, characterized in that it is divided into
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| WO2024117733A1 (en) * | 2022-11-28 | 2024-06-06 | 주식회사 바이오로지텍 | Method for cultivating cordyceps militaris with high content of collagen and cordycepin, and method for extracting high-purity collagen and cordycepin |
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| KR20130057507A (en) | 2011-11-24 | 2013-06-03 | 한라산동충하초영농조합법인 | A method of cultivation cordyceps militaris |
| KR20160033911A (en) | 2014-09-19 | 2016-03-29 | (주)유비쿼터스통신 | The method for automatic cultivation of Cordyceps militaris and cultivated Cordyceps militaris |
| KR20190101792A (en) | 2018-02-23 | 2019-09-02 | 주식회사 바이오아라 | Manufacturing Method for Cordyceps with Increased Content of Cordycepin and Cordyceps Prepared by the Method |
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| KR20130057507A (en) | 2011-11-24 | 2013-06-03 | 한라산동충하초영농조합법인 | A method of cultivation cordyceps militaris |
| KR20160033911A (en) | 2014-09-19 | 2016-03-29 | (주)유비쿼터스통신 | The method for automatic cultivation of Cordyceps militaris and cultivated Cordyceps militaris |
| KR20190101792A (en) | 2018-02-23 | 2019-09-02 | 주식회사 바이오아라 | Manufacturing Method for Cordyceps with Increased Content of Cordycepin and Cordyceps Prepared by the Method |
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| WO2024117733A1 (en) * | 2022-11-28 | 2024-06-06 | 주식회사 바이오로지텍 | Method for cultivating cordyceps militaris with high content of collagen and cordycepin, and method for extracting high-purity collagen and cordycepin |
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