KR20200069778A - Antioxidant composition containing femented Rubus coreanus extract - Google Patents
Antioxidant composition containing femented Rubus coreanus extract Download PDFInfo
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- KR20200069778A KR20200069778A KR1020180157223A KR20180157223A KR20200069778A KR 20200069778 A KR20200069778 A KR 20200069778A KR 1020180157223 A KR1020180157223 A KR 1020180157223A KR 20180157223 A KR20180157223 A KR 20180157223A KR 20200069778 A KR20200069778 A KR 20200069778A
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- South Korea
- Prior art keywords
- extract
- fermentation
- bokbunja
- antioxidant composition
- bokbunja extract
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
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Abstract
Description
본 발명은 발효 전 복분자 추출물보다 항산화 효과보다 향상된 발효복분자추출물을 유효성분으로 포함하는 항산화 조성물에 관한 것이다.The present invention relates to an antioxidant composition comprising as an active ingredient a fermented bokbunja extract that is more improved than the antioxidant effect than bokbunja extract before fermentation.
생체가 살아가기 위해서는 영양분의 섭취는 물론, 활발하게 호흡을 하며 이 호흡을 통하여 들어간 산소가 영양성분을 연소시켜 에너지를 방출함으로써 육체적인 운동을 포함한 두뇌활동도 가능하게 되고, 이 때 소량의 활성산소종(ROS)들과 반응성이 큰 유리기가 생성되는데, 신체가 노화되거나 기타 내외적인 요인에 의해서 활성산소 및/또는 유리기에 의한 조직손상이 야기된다. 산화적 스트레스는 일반적으로 활성산소종(Reactive oxygen species, ROS)을 생산하고 제거하는 세포생화학적 프로세스 사이의 불균형인 것으로 알려져 있고, 신경세포에서 ROS의 축적은 신경병성 장애의 발병에서 중요한 역할을 한다. 활성산소는 단백질의 SH기와 반응하여 효소의 활성을 잃게 하거나 가교결합의 형성, DNA, RNA, 효소 및 세포막을 구성하는 불포화 지방산을 산화시켜 과산화지질을 형성하게 된다. 이러한 과산화지질은 생체에 매우 유독하여 동맥경화, 혈액순환장애, 피로, 당뇨병, 심장병, 간장장애, 발암, 암전이 등과 같은 질병의 원인이 된다. 이러한 지질의 과산화를 막아주는 물질을 항산화물질이라 한다.In order for the living body to live, it is possible not only to consume nutrients, but also to breathe vigorously, and oxygen, which enters through this breath, burns nutrients and releases energy, thereby enabling brain activities including physical exercise, and in this case, a small amount of free radicals Free radicals that are highly reactive with species (ROS) are produced, causing tissue damage due to free radicals and/or free radicals due to aging or other internal and external factors. Oxidative stress is generally known to be an imbalance between the cell biochemical processes that produce and remove reactive oxygen species (ROS), and the accumulation of ROS in neurons plays an important role in the development of neuropathic disorders. . Reactive oxygen reacts with the SH group of the protein to lose the activity of the enzyme or to form cross-linking, oxidize DNA, RNA, enzymes, and unsaturated fatty acids that make up the cell membrane to form lipid peroxide. These lipid peroxides are very toxic to living organisms and cause diseases such as arteriosclerosis, blood circulation disorders, fatigue, diabetes, heart disease, liver failure, carcinogenesis, and cancer metastasis. A substance that prevents lipid peroxidation is called antioxidant.
한편, 복분자는 6월 내지 8월에 수확되고, 음력 5월 즉, 6월 또는 7월 초에 익은 열매가 주로 검붉은색을 띠고 있다. 상기 복분자는 작은 단과가 여러 개 모영서 덩어리를 이룬 것으로, 원추형이나 눌러진 구형을 이루고 있다. 또한, 복분자는 다량의 폴리페놀을 함유하고 있어 항암효과, 동맥경화예방, 혈전 예방, 항염증 효과, 헬리코박터 파이로리에 대한 항균효과 및 항산화 효과가 있는 것으로 보고되고 있다.On the other hand, the bokbunja is harvested in June to August, and the ripe fruits in the lunar May, that is, in early June or early July, have a dark red color. The bokbunja is composed of several small fruits and lumps, forming a conical or pressed sphere. In addition, since the abdominal molecule contains a large amount of polyphenols, it has been reported to have anti-cancer effects, prevention of arteriosclerosis, thrombus prevention, anti-inflammatory effects, and antibacterial and antioxidant effects against Helicobacter pylori.
본 발명은 항산화 효과가 우수한 발효복분자추출물을 유효성분으로 포함하는 항산화 조성물을 제공하는데 목적이 있다.An object of the present invention is to provide an antioxidant composition comprising a fermented bokbunja extract with excellent antioxidant effect as an active ingredient.
또한, 본 발명은 본 발명에 따른 발효복분자추출물을 포함하는 항산화 조성물을 포함하는 항산화용 건강강기능식품 및 화장품을 제공하는데 또 다른 목적이 있다.In addition, the present invention has another object to provide a health functional food and cosmetics for antioxidants containing an antioxidant composition comprising a fermented bokbunja extract according to the present invention.
본 발명은 비피도박테리움 롱검(Bifidobacterium longum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 락토바실러스 플랜타럼(Lactobacillus plantarum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 및 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 로 이루어진 군에서 선택된 어느 하나의 균주를 복분자 추출물에 접종하여 발효시킴으로써 수득된 발효복분자추출물을 유효성분으로 포함하는 항산화 조성물을 제공한다.The present invention is Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus plantarum, Streptococcus thermophilus, and Enterococcus tylandicus thailand ) It provides an antioxidant composition comprising a fermented bokbunja extract obtained by inoculating any one strain selected from the group consisting of bokbunja extract to fermentation.
본 발명의 일 실시예에 의하면, 상기 복분자 추출물은 추출 용매로 물을 이용하여 추출되고, 상기 발효복분자추출물은 상기 복분자 추출물에 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 또는 스트렙코커스 써모필러스(Streptococcus thermophilus) 균주를 접종하여 발효시킴으로써 수득된 것일 수 있다.According to an embodiment of the present invention, the bokbunja extract is extracted using water as an extraction solvent, and the fermentation bokbunja extract is enterococcus tyrandisus (Enterococcus thailandicus) or streptococcus thermophilus on the bokbunja extract. ) May be obtained by inoculating the strain and fermenting.
또한 본 발명의 일 실시예에 의하면, 상기 발효복분자추출물은 150~450 ㎍/ml의 농도로 포함될 수 있다.In addition, according to an embodiment of the present invention, the fermentation complex extract may be included in a concentration of 150 ~ 450 ㎍ / ml.
또한 본 발명의 일 실시예에 의하면, 상기 복분자 추출물은 추출 용매로 물을 이용하여 추출되고, 상기 발효복분자추출물은 상기 복분자 추출물에 비피도박테리움 롱검(Bifidobacterium longum) 균주를 접종하여 발효시킴으로써 수득된 것이고, 상기 조성물 내 170~250 ㎍/ml의 농도로 포함될 수 있다.In addition, according to an embodiment of the present invention, the bokbunja extract is extracted using water as an extraction solvent, the fermentation bokbunja extract is bifidobacterium longum ( Bifidobacterium) in the bokbunja extract longum ) is obtained by inoculating the strain, and may be included at a concentration of 170-250 μg/ml in the composition.
또한 본 발명의 일 실시예에 의하면, 상기 복분자 추출물은 추출 용매로 물을 이용하여 추출되고, 상기 발효복분자추출물은 상기 복분자 추출물에 락토바실러스 플랜타럼(Lactobacillus plantarum) 균주를 접종하여 발효시킴으로써 수득될 수 있다.In addition, according to an embodiment of the present invention, the bokbunja extract is extracted using water as an extraction solvent, the fermentation bokbunja extract can be obtained by inoculating the bokbunja extract Lactobacillus plant tarum (Lactobacillus plantarum) strain have.
또한 본 발명의 일 실시예에 의하면, 상기 발효복분자추출물은 170~450 ㎍/ml의 농도로 포함될 수 있다.In addition, according to an embodiment of the present invention, the fermentation complex extract may be included in a concentration of 170 ~ 450 ㎍ / ml.
또한 본 발명의 일 실시예에 의하면, 상기 복분자 추출물은 추출 용매로 20~40%(v/v) 주정을 이용하여 추출될 수 있다.In addition, according to an embodiment of the present invention, the bokbunja extract can be extracted using 20-40% (v/v) alcohol as an extraction solvent.
또한 본 발명의 일 실시예에 의하면, 상기 발효복분자추출물은 20~250 ㎍/ml의 농도로 포함될 수 있다.In addition, according to an embodiment of the present invention, the fermentation complex extract may be included in a concentration of 20 ~ 250 ㎍ / ml.
또한 본 발명의 일 실시예에 의하면, 상기 복분자 추출물은 추출 용매로 60~80%(v/v) 주정을 이용하여 추출될 수 있다.In addition, according to an embodiment of the present invention, the bokbunja extract can be extracted using 60-80% (v/v) alcohol as an extraction solvent.
또한 본 발명의 일 실시예에 의하면, 상기 발효복분자추출물은 상기 복분자 추출물에 비피도박테리움 롱검(Bifidobacterium longum) 균주를 접종하여 발효시킴으로써 수득된 것이고, 상기 조성물 내 80~250 ㎍/ml의 농도로 포함될 수 있다.In addition, according to an embodiment of the present invention, the fermented bokbunja extract is Bifidobacterium long gum in the bokbunja extract longum ) is obtained by inoculating the strain and may be included at a concentration of 80 to 250 μg/ml in the composition.
또한 본 발명의 일 실시예에 의하면, 상기 발효복분자추출물은 상기 복분자 추출물에 락토바실러스 헬베티커스(Lactobacillus helveticus), 락토바실러스 플랜타럼(Lactobacillus plantarum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 및 엔테로코커스 타이랜디커스(Enterococcus thailandicus)으로 이루어진 군에서 선택된 어느 하나의 균주를 접종하여 발효시킴으로써 수득된 것이고, 상기 조성물 내 20~250 ㎍/ml의 농도로 포함될 수 있다.In addition, according to an embodiment of the present invention, the fermented bokbunja extract is Lactobacillus helveticus, Lactobacillus plantarum, Streptococcus thermophilus, and Enterococcus on the bokbunja extract. Tyrandiscus (Enterococcus thailandicus) is obtained by inoculating and fermenting any one strain selected from the group consisting of, it may be included in a concentration of 20 ~ 250 ㎍ / ml in the composition.
또한 본 발명의 일 실시예에 의하면, 상기 비피도박테리움 롱검(Bifidobacterium longum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 락토바실러스 플랜타럼(Lactobacillus plantarum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 및 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 균주의 기탁번호는 각각 KACC91563, KACC91148, KACC91146, KACC91147 및 KACC92253P일 수 있다.In addition, according to an embodiment of the present invention, the Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus plantarum, Streptococcus thermophilus, and Enterococcus thailandicus (Enterococcus thailandicus) may have a deposit number of KACC91563, KACC91148, KACC91146, KACC91147 and KACC92253P, respectively.
또한 본 발명은 본 발명에 따른 항산화 조성물을 포함하는 항산화용 건강기능식품을 제공한다.In addition, the present invention provides an antioxidant health functional food comprising the antioxidant composition according to the present invention.
또한 본 발명은 본 발명에 따른 항산화 조성물을 포함하는 항산화용 화장품을 제공한다.In addition, the present invention provides an antioxidant cosmetic product comprising the antioxidant composition according to the present invention.
본 발명에 따른 항산화 조성물은 발효 전 복분자 추출물보다의 항산화 효과보다 현저히 향상된 발효복분자추출물을 포함함으로써 항산화용 건강기능식품으로 널리 활용될 수 있다.Antioxidant composition according to the present invention can be widely used as a health functional food for antioxidant by including a fermented bokbunja extract significantly improved than the antioxidant effect than the bokbunja extract before fermentation.
도 1a는 추출 용매로 물을 사용하여 추출된 복분자 추출물에 락토바실러스 플랜타럼(Lactobacillus plantarum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 비피도박테리움 롱검(Bifidobacterium longum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 및 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 균주를 접종하여 발효된 각각의 발효 복분자 추출물의 농도에 따른 DPPH 라디칼 소거능 증가율을 도시한 그래프이다.
도 1b는 추출 용매로 30%(v/v) 주정을 사용하여 추출된 복분자 추출물에 락토바실러스 플랜타럼(Lactobacillus plantarum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 비피도박테리움 롱검(Bifidobacterium longum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 또는 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 균주를 접종하여 발효된 각각의 발효 복분자 추출물의 농도에 따른 DPPH 라디칼 소거능 증가율을 도시한 그래프이다.
도 1c는 추출 용매로 70%(v/v) 주정을 사용하여 추출된 복분자 추출물에 락토바실러스 플랜타럼(Lactobacillus plantarum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 비피도박테리움 롱검(Bifidobacterium longum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 또는 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 균주를 접종하여 발효된 각각의 발효 복분자 추출물의 농도에 따른 DPPH 라디칼 소거능 증가율을 도시한 그래프이다.Figure 1a is Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus helveticus, Bifidobacterium longum, B. Streptococcus thermophilus) and Enterococcus thailandicus (Enterococcus thailandicus) is a graph showing the DPPH radical scavenging activity increase rate according to the concentration of each fermented bokbunja extract fermented by inoculation.
FIG. 1b shows Lactobacillus plantarum, Lactobacillus helveticus, Bifidobacterium longum on a bimodal extract extracted using 30% (v/v) alcohol as an extraction solvent. , Streptococcus thermophilus or Enterococcus thailandicus strain is a graph showing the increase rate of DPPH radical scavenging activity according to the concentration of each fermented bokbunja extract fermented by inoculation.
Figure 1c is a Lactobacillus plantarum, Lactobacillus helveticus, Bifidobacterium longum, on the extract of a bokbunja extract using 70% (v/v) alcohol as an extraction solvent. , Streptococcus thermophilus or Enterococcus thailandicus strain is a graph showing the increase rate of DPPH radical scavenging activity according to the concentration of each fermented bokbunja extract fermented by inoculation.
본 발명의 명세서에 있어서, 용어 “조성물(composition)”은 본 발명의 황기 추출물에 희석제 또는 담체와 같은 다른 화학 성분들을 혼합한 혼합물을 의미한다. In the context of the present invention, the term "composition (composition)" refers to a mixture of other chemical components such as diluents or carriers in the astragalus extract of the present invention.
본 발명에서 사용되는 모든 기술용어는 달리 정의되지 않는 이상, 본 발명의 관련 분야에서 통상의 당업자가 일반적으로 이해하는 바와 같은 의미로 사용된다. 또한 본 명세서에는 바람직한 방법이나 시료가 기재되나, 이와 유사하거나 동등한 것들도 본 발명의 범주에 포함된다.All technical terms used in the present invention are used in the meaning as generally understood by those skilled in the art in the relevant field of the present invention, unless otherwise defined. Also, a preferred method or sample is described herein, but similar or equivalent ones are included in the scope of the present invention.
이하, 첨부한 도면을 참고로 하여 본 발명의 실시예에 대하여 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 상세히 설명한다. 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며 여기에서 설명하는 실시예에 한정되지 않는다.Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art to which the present invention pertains can easily practice. The present invention can be implemented in many different forms and is not limited to the embodiments described herein.
본 발명에 따른 항산화 조성물은 비피도박테리움 롱검(Bifidobacterium longum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 락토바실러스 플랜타럼(Lactobacillus plantarum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 및 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 로 이루어진 군에서 선택된 어느 하나의 균주를 복분자 추출물에 접종하여 발효시킴으로써 수득된 발효복분자추출물을 유효성분으로 포함한다.Antioxidant compositions according to the invention are Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus plantarum, Streptococcus thermophilus and Enterococcus typhilis A fermented bokbunja extract obtained by inoculating a bokbunja extract with one strain selected from the group consisting of Cucus (Enterococcus thailandicus) and fermenting it as an active ingredient.
또한 상기 비피도박테리움 롱검(Bifidobacterium longum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 락토바실러스 플랜타럼(Lactobacillus plantarum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 및 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 균주의 기탁번호는 각각 KACC91563, KACC91148, KACC91146, KACC91147 및 KACC92253P일 수 있다.In addition, the Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus plantarum, Streptococcus thermophilus and Enterococcus tylandusicus enterococcus thailand The deposit number of the strain may be KACC91563, KACC91148, KACC91146, KACC91147 and KACC92253P, respectively.
본 발명에 따른 항산화 조성물에 포함되는 발효복분자추출물은 복분자 추출물 제조 시 사용되는 용매, 발효 시 사용되는 균주, 항산화 조성물 내 포함되는 농도에 따라 항산화 특성이 현저히 달라질 수 있다.The fermented bokbunja extract contained in the antioxidant composition according to the present invention may have significantly different antioxidant properties depending on the solvent used when preparing the bokbunja extract, the strain used during fermentation, and the concentration contained in the antioxidant composition.
상기 복분자 추출물은 당업계에서 통상적으로 사용되는 용매추출법을 사용하여 제조될 수 있으며, 일예로 가열추출법일 수 있으나 이에 제한되지 않는다.The bokbunja extract may be prepared using a solvent extraction method commonly used in the art, and may be, for example, a heating extraction method, but is not limited thereto.
상기 복분자 추출물 제조 시 추출 용매로 물을 이용하여 추출될 경우, 50~90℃의 온도에서 2~8 시간동안 수행될 수 있으며, 더욱 바람직하게는 상기 추출은 2~5회 반복하여 수행될 수 있다. 상기 열처리 반복 수행 시 목적하는 온도에 도달한 후 유지되고, 상온으로 냉각한 후에 다시 열처리가 시작될 수 있다.When extracting using the water as an extraction solvent in the preparation of the extract of the bokbunja extract, it may be performed for 2 to 8 hours at a temperature of 50 to 90°C, more preferably, the extraction may be performed repeatedly 2 to 5 times. . When the heat treatment is repeatedly performed, it is maintained after reaching a desired temperature, and after cooling to room temperature, heat treatment may be started again.
또한, 본 발명의 일 실시예에 따른 항산화 조성물은 추출 용매로 물을 이용하여 추출된 복분자 추출물에 락토바실러스 플랜타럼(Lactobacillus plantarum), 스트렙코커스 써모필러스(Streptococcus thermophilus), 비피도박테리움 롱검(Bifidobacterium longum) 및 락토바실러스 헬베티커스(Lactobacillus helveticus) 로 이루어진 군에서 선택된 어느 하나의 균주를 접종하여 발효시킴으로써 수득된 발효복분자추출물을 포함할 수 있으며, 이 경우 발효 전 복분자추출물보다 항산화 효과가 현저히 향상될 수 있다. In addition, the antioxidant composition according to an embodiment of the present invention is Lactobacillus plantarum, Streptococcus thermophilus, Bifidobacterium long gum (Lactobacillus plantarum) in the extract of bokbunja extracted using water as an extraction solvent Bifidobacterium longum) and Lactobacillus helveticus (Lactobacillus helveticus) may contain a fermentation complex extract obtained by inoculating and fermenting any one strain selected from the group consisting of, in this case, the antioxidant effect is significantly improved than the fermentation extract before fermentation Can be.
상기 복분자 추출물 발효 시, 발효는 30~40℃의 온도에서 48~96시간 동안 수행될 수 있다.Upon fermentation of the bokbunja extract, fermentation may be performed at a temperature of 30-40° C. for 48-96 hours.
상기 복분자 추출물은 추출 용매로 물을 이용하고, 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 또는 스트렙코커스 써모필러스(Streptococcus thermophilus) 균주를 접종하여 발효될 경우, 발효복분자추출물은 170~450 ㎍/ml, 더욱 바람직하게는 170~250㎍/ml의 농도로 항산화 조성물 내 포함되어 항산화 효과가 더욱 향상될 수 있다.When the bokbunja extract is fermented by inoculating strains of Enterococcus thailandicus or Streptococcus thermophilus, the fermentation bokbunja extract is 170-450 μg/ml, More preferably, the antioxidant effect may be further improved by being included in the antioxidant composition at a concentration of 170 to 250 μg/ml.
또한, 상기 복분자 추출물은 추출 용매로 물을 이용하고, 비피도박테리움 롱검(Bifidobacterium longum) 균주를 접종하여 발효될 경우, 발효복분자추출물은 20~450 ㎍/ml, 더욱 바람직하게는 170~450㎍/ml, 가장 바람직하게는 170~250㎍/ml의 농도로 항산화 조성물 내 포함되어 항산화 효과가 더욱 향상될 수 있다.In addition, when the fermentation extract is fermented by using water as an extraction solvent and inoculating a strain of Bifidobacterium longum, the fermentation complex extract is 20-450 µg/ml, more preferably 170-450 µg /ml, most preferably included in the antioxidant composition at a concentration of 170 ~ 250㎍ / ml can further improve the antioxidant effect.
또한, 본 발명의 다른 일 실시예에 따른 항산화 조성물은 추출 용매로 20~40%(v/v) 주정을 이용하여 추출될 수 있으며, 이에 따라 발효 전 복분자 추출물 대비 발효복분자추출물의 항산화 효과가 현저히 향상될 수 있다.In addition, the antioxidant composition according to another embodiment of the present invention can be extracted by using 20-40% (v/v) alcohol as an extraction solvent, so that the antioxidant effect of the fermented bokbunja extract compared to the bokbunja extract before fermentation is remarkably Can be improved.
상기 복분자 추출물은 추출 용매로 20~40%(v/v) 주정을 이용하여 추출될 경우, 복분자추출물은 20~250 ㎍/ml, 더욱 바람직하게는 20~60㎍/ml의 농도로 항산화 조성물 내 포함되어 항산화 효과가 더욱 향상될 수 있다.When the bokbunja extract is extracted using 20-40% (v/v) alcohol as an extraction solvent, the bokbunja extract is in an antioxidant composition at a concentration of 20-250 µg/ml, more preferably 20-60 µg/ml. It can be included to further improve the antioxidant effect.
상기 복분자 추출물 제조 시 추출 용매로 20~40%(v/v) 주정을 이용하여 추출될 경우, 50~90℃의 온도에서 2~8 시간동안 수행될 수 있으며, 더욱 바람직하게는 상기 추출은 2~5회 반복하여 수행될 수 있다. 상기 열처리 반복 수행 시 목적하는 온도에 도달한 후 유지되고, 상온으로 냉각한 후에 다시 열처리가 시작될 수 있다.When the extract is made using 20-40% (v/v) alcohol as an extraction solvent when preparing the extract of the bokbunja extract, it may be performed for 2 to 8 hours at a temperature of 50 to 90°C, more preferably, the extraction is 2 It can be performed by repeating ~5 times. When the heat treatment is repeatedly performed, it is maintained after reaching a desired temperature, and after cooling to room temperature, heat treatment may be started again.
또한, 본 발명의 또 다른 일 실시예에 따른 항산화 조성물은 추출 용매로 60~80%(v/v) 주정을 이용하여 추출될 수 있으며, 이에 따라 발효 전 복분자 추출물 대비 발효복분자추출물의 항산화 효과가 현저히 향상될 수 있다.In addition, the antioxidant composition according to another embodiment of the present invention can be extracted using 60-80% (v/v) alcohol as an extraction solvent, so that the antioxidant effect of the fermented bokbunja extract compared to the bokbunja extract before fermentation It can be significantly improved.
상기 복분자 추출물 제조 시 추출 용매로 60~80%(v/v) 주정을 이용하여 추출될 경우, 50~90℃의 온도에서 2~8 시간동안 수행될 수 있으며, 더욱 바람직하게는 상기 추출은 2~5회 반복하여 수행될 수 있다. 상기 열처리 반복 수행 시 목적하는 온도에 도달한 후 유지되고, 상온으로 냉각한 후에 다시 열처리가 시작될 수 있다.In the case of extraction with 60-80% (v/v) alcohol as an extraction solvent when preparing the extract of the bokbunja extract, it may be performed for 2 to 8 hours at a temperature of 50 to 90°C, more preferably, the extraction is 2 It can be performed by repeating ~5 times. When the heat treatment is repeatedly performed, it is maintained after reaching a desired temperature, and after cooling to room temperature, heat treatment may be started again.
상기 복분자 추출물은 추출 용매로 60~80%(v/v) 주정을 이용하고, 비피도박테리움 롱검(Bifidobacterium longum) 균주를 접종하여 발효될 경우, 발효복분자추출물은 80~250 ㎍/ml의 농도로 항산화 조성물 내 포함되어 항산화 효과가 더욱 향상될 수 있다.When the bokbunja extract is fermented using 60~80% (v/v) alcohol as an extraction solvent, and inoculated with a strain of Bifidobacterium longum, the fermentation bokbunja extract has a concentration of 80-250 μg/ml As it is included in the antioxidant composition, the antioxidant effect may be further improved.
또한 상기 복분자 추출물은 추출 용매로 60~80%(v/v) 주정을 이용하고, 락토바실러스 헬베티커스(Lactobacillus helveticus) 균주를 접종하여 발효될 경우, 발효복분자추출물은 20~250 ㎍/ml, 더욱 바람직하게는 20~150㎍/ml, 가장 바람직하게는 70~150㎍/ml의 농도로 항산화 조성물 내 포함되어 항산화 효과가 더욱 향상될 수 있다.In addition, when the fermentation extract is fermented by using 60-80% (v/v) alcohol as an extraction solvent, and inoculating a strain of Lactobacillus helveticus, the fermentation complex extract is 20-250 μg/ml, More preferably, the antioxidant effect may be further improved by being included in the antioxidant composition at a concentration of 20 to 150 μg/ml, most preferably 70 to 150 μg/ml.
또한 상기 복분자 추출물은 추출 용매로 60~80%(v/v) 주정을 이용하고, 락토바실러스 플랜타럼(Lactobacillus plantarum) 균주를 접종하여 발효될 경우, 발효복분자추출물은 20~250 ㎍/ml, 더욱 바람직하게는 20~150㎍/ml, 가장 바람직하게는 70~150㎍/ml의 농도로 항산화 조성물 내 포함되어 항산화 효과가 더욱 향상될 수 있다.In addition, when the fermentation extract is fermented by using 60-80% (v/v) alcohol as an extraction solvent, and inoculating the Lactobacillus plantarum strain, the fermentation complex extract is 20-250 μg/ml, more Preferably 20 ~ 150㎍ / ml, most preferably included in the antioxidant composition at a concentration of 70 ~ 150㎍ / ml, the antioxidant effect can be further improved.
또한 상기 복분자 추출물은 추출 용매로 60~80%(v/v) 주정을 이용하고, 스트렙코커스 써모필러스(Streptococcus thermophilus) 균주를 접종하여 발효될 경우, 발효복분자추출물은 20~250 ㎍/ml, 더욱 바람직하게는 20~150㎍/ml, 가장 바람직하게는 70~150㎍/ml의 농도로 항산화 조성물 내 포함되어 항산화 효과가 더욱 향상될 수 있다.In addition, when the fermentation extract is fermented by inoculating the strain Streptococcus thermophilus using 60-80% (v/v) alcohol as an extraction solvent, the fermentation complex extract is 20-250 μg/ml, More preferably, the antioxidant effect may be further improved by being included in the antioxidant composition at a concentration of 20 to 150 μg/ml, most preferably 70 to 150 μg/ml.
또한 상기 복분자 추출물은 추출 용매로 60~80%(v/v) 주정을 이용하고, 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 균주를 접종하여 발효될 경우, 발효복분자추출물은 20~250 ㎍/ml, 더욱 바람직하게는 70~150㎍/ml의 농도로 항산화 조성물 내 포함되어 항산화 효과가 더욱 향상될 수 있다.In addition, when the fermentation extract is fermented by using 60-80% (v/v) alcohol as an extraction solvent and inoculating a strain of Enterococcus thailandicus, the fermentation complex extract is 20-250 μg/ml, More preferably, the antioxidant effect may be further improved by being included in the antioxidant composition at a concentration of 70 to 150 μg/ml.
다음으로 본 발명에 따른 항산화 조성물을 포함하는 건강기능식품에 대하여 설명한다.Next, a health functional food containing the antioxidant composition according to the present invention will be described.
상기 건강기능식품 조성물의 종류에는 통상적으로 제조 및/또는 판매되는 것이라면 특별히 제한하지 않는다. 예를 들면, 육류, 소시지, 빵, 초콜릿, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알코올음료 및 비타민 복합제 등이 있으며, 환제, 분말, 과립, 침제, 정제, 캡슐 또는 음료인 형태로 사용할 수 있고 통상적인 의미에서의 건강기능식품을 모두 포함한다.The type of the health functional food composition is not particularly limited as long as it is usually manufactured and/or sold. For example, dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, beverages, teas, drinks, alcoholic beverages and vitamin complexes It can be used in the form of pills, powders, granules, needles, tablets, capsules, or beverages, and includes all health functional foods in the ordinary sense.
상기 건강기능식품이 음료 조성물일 경우, 본 발명에 따른 항산화 조성물을 함유하는 것 외에는 액체성분에는 특별한 제한은 없으며 통상의 음료와 같이 여러가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다.When the health functional food is a beverage composition, there is no particular limitation on the liquid component other than containing the antioxidant composition according to the present invention, and it may contain various flavoring agents or natural carbohydrates, etc., as additional components, like a conventional beverage.
통상적으로, 건강기능식품에 포함되는 발효복분자추출물의 양은 전체 식품 중량의 0.01~20 중량%, 바람직하게는 0.01~10 중량%로 포함될 수 있다. 또한, 건강 및 위생을 목적으로 하거나 또는 건강 조절을 목적으로 하는 장기간의 섭취의 경우에는 상기 범위 이하일 수 있으며, 안전성 면에서 아무런 문제가 없기 때문에 유효성분은 상기 범위 이상의 양으로도 사용할 수도 있다.Typically, the amount of fermented bokbunja extract contained in the health functional food may be included in 0.01 to 20% by weight of the total food weight, preferably 0.01 to 10% by weight. In addition, in the case of long-term intake for the purpose of health and hygiene or for health control, it may be below the above range, and since there is no problem in terms of safety, the active ingredient may also be used in an amount above the above range.
또한, 본 발명에 따른 항산화 조성물을 유효성분으로 포함하는 항산화용 화장품에 대하여 설명한다.In addition, an antioxidant cosmetic composition containing the antioxidant composition according to the present invention as an active ingredient will be described.
화장료 조성물로 제제화되는 경우, 상기 항염증 조성물의 함량은 화장료 조성물 총 중량에 대하여 0.0001 내지 10 중량%이며, 바람직하게는 0.01 내지 5.0 중량%이다. 최소한의 자외선에 의한 피부 손상 개선 효과를 달성할 수 있도록 발효 복분자 추출물의 함량은 상기 최소치 이상인 것이 바람직하며, 과량 첨가에 따른 사용감 저하 및 각종 제형에의 적용 가능성을 고려하여 발효 복분자 추출물은 상기 최대치 이하인 것이 바람직하다. 이때, 상기 발효 복분자 추출물의 함량은 제형 또는 화장료 조성물에 함유되는 성분들의 함량에 따라 상기 범위 내에서 적절히 조절될 수 있다.When formulated as a cosmetic composition, the content of the anti-inflammatory composition is 0.0001 to 10% by weight relative to the total weight of the cosmetic composition, preferably 0.01 to 5.0% by weight. In order to achieve the effect of improving skin damage caused by minimal ultraviolet rays, the content of the fermented bokbunja extract is preferably greater than or equal to the above minimum, and the fermentation bokbunja extract is less than or equal to the maximum, taking into account the reduced usability due to excessive addition and the applicability to various formulations It is preferred. At this time, the content of the fermented bokbunja extract can be appropriately adjusted within the above range according to the content of the ingredients contained in the formulation or cosmetic composition.
이하, 본 발명의 항염증용 조성물을 함유하는 건강기능식품의 제조예를 설명하나, 본 발명은 이를 한정하고자 함이 아닌 단지 구체적으로 설명하고자 함이다.Hereinafter, an example of preparing a health functional food containing the anti-inflammatory composition of the present invention will be described, but the present invention is intended to be described in detail rather than to limit it.
<제조예 1><Production Example 1>
발효복분자추출물 200 ㎎Fermented
비타민 A 아세테이트 70 ㎍Vitamin A Acetate 70 μg
비타민 E 1.0 ㎎Vitamin E 1.0 mg
비타민 B 1 0.13 ㎎Vitamin B 1 0.13 mg
비타민 B 2 0.15 ㎎Vitamin B 2 0.15 mg
비타민 B 6 0.5㎎Vitamin B 6 0.5mg
비타민 B 12 0.2 ㎍Vitamin B 12 0.2 μg
비타민 C 10 ㎎Vitamin C 10 mg
비오틴10 ㎍Biotin10 ㎍
니코틴산아미드 1.7 ㎎Nicotinic acid amide 1.7 mg
엽산 50 ㎍50 ㎍ folic acid
판토텐산 칼슘 0.5 ㎎Calcium Pantothenate 0.5 mg
황산제1철 1.75 ㎎Ferrous sulfate 1.75 mg
산화아연 0.82 ㎎Zinc oxide 0.82 mg
탄산마그네슘 25.3 ㎎Magnesium carbonate 25.3 mg
제1인산칼륨 15 ㎎Potassium phosphate 15 mg
제2인산칼슘 55 ㎎Dibasic calcium phosphate 55 mg
구연산칼륨 90 ㎎Potassium citrate 90 mg
탄산칼슘 100 ㎎
염화마그네슘 24.8 ㎎Magnesium chloride 24.8 mg
이하, 본 발명의 항산화용 화장품의 제조예를 설명하나, 본 발명은 이를 한정하고자 함이 아닌 단지 구체적으로 설명하고자 함이다.Hereinafter, an example of manufacturing the cosmetic composition for antioxidant of the present invention will be described, but the present invention is intended to be described in detail rather than to limit it.
<제조예2: 크림><Production Example 2: Cream>
항산화 조성물을 포함하는 크림을 통상의 방법에 따라 제조하였다.Creams containing antioxidant compositions were prepared according to conventional methods.
발효 복분자 추출물: 1중량%Fermented bokbunja extract: 1% by weight
친유형 모노스테아린산글리세린: 2중량%Lipophilic glycerin monostearate: 2% by weight
세테아릴알콜: 2중량%Cetearyl alcohol: 2% by weight
스테아린산: 1.5중량%Stearic acid: 1.5% by weight
폴리솔베이트 60: 1.5중량%Polysorbate 60: 1.5% by weight
솔비탄스테아레이트: 0.6중량%Sorbitan stearate: 0.6% by weight
하이드로제네이티드 폴리이소부텐: 1중량%Hydrogenated polyisobutene: 1% by weight
스쿠알란: 3중량%Squalane: 3% by weight
광물유: 5중량%Mineral oil: 5% by weight
사이클로메치콘: 5중량%Cyclomethicone: 5% by weight
디메치콘: 1중량%Dimethicone: 1% by weight
초산토코페롤: 0.5중량%Tocopherol acetate: 0.5% by weight
글리세린: 5중량%Glycerin: 5% by weight
베타인: 3중량%Betaine: 3% by weight
트리에탄올아민: 1중량%Triethanolamine: 1% by weight
산탄검: 0.05중량%Xanthan gum: 0.05% by weight
향: 적량Incense: suitable
방부제: 적량Preservative: suitable
색소: 적량Pigmentation: suitable amount
증류수: 잔량Distilled water: balance
이하 하기 실시예를 통해 본 발명을 보다 상세히 설명한다. 그러나 하기 실시예는 본 발명의 기술적 사상의 내용과 범위를 쉽게 설명하기 위한 예시일 뿐, 이에 의해 본 발명의 기술적 범위가 한정되거나 변경되는 것은 아니다. 또한 이러한 예시에 기초하여 본 발명의 기술적 사상의 범위 안에서 다양한 변형과 변경이 가능함은 당업자에 의해 용이하게 결정될 수 있다.The present invention will be described in more detail through the following examples. However, the following examples are merely examples for easily explaining the contents and scope of the technical spirit of the present invention, and the technical scope of the present invention is not limited or changed thereby. In addition, various modifications and changes are possible within the scope of the technical spirit of the present invention based on these examples can be easily determined by those skilled in the art.
(실시예 1)(Example 1)
건조된 복분자를 분쇄하여 수득한 복분자 분말을 증류수에 첨가한 후, 70℃의 온도에서 4시간 동안 3회 반복하여 가열추출하였다. 추출에 사용된 용매는 진공여과법을 통해 제거되었다. 수득된 복분자 추출물에 농촌진흥청 국립농업과학원(KACC)으로부터 분양받은 락토바실러스 플랜타럼(Lactobacillus plantarum) 균주(기탁번호:KACC91147)를 복분자 추출물에 접종하고 35±2℃에서 72시간 동안 발효하여 발효복분자추출물을 수득하였다.After adding the obtained bokbunja powder by pulverizing the dried bokbunja to distilled water, the mixture was heated and extracted three times for 4 hours at a temperature of 70°C. The solvent used for extraction was removed by vacuum filtration. The obtained bokbunja extract was inoculated with bokbunja extract (Lactobacillus plantarum) strain (Accession No.: KACC91147), which was pre-sold by the National Academy of Agricultural Science (KACC), from the Rural Development Administration and fermented bokbunja extract by fermentation at 35±2℃ for 72 hours. Was obtained.
(실시예 2~15)(Examples 2 to 15)
실시예1과 동일하게 실시하되, 하기 표 1에 기재된 바와 같이 추출용매 및/또는 발효에 사용되는 균주를 달리하여 발효복분자추출물을 수득하였다.The same procedure as in Example 1 was carried out, but as described in Table 1 below, the extractant and/or the strain used for fermentation were varied to obtain a fermentation complex extract.
(비교예 1)(Comparative Example 1)
실시예1과 동일하게 실시하되, 발효하지 않은 복분자 추출물을 준비하였다.The same procedure as in Example 1 was performed, but a non-fermented bokbunja extract was prepared.
(비교예 2)(Comparative Example 2)
실시예1과 동일하게 실시하되, 추출 용매로 30%(v/v) 주정을 사용하고, 발효하지 않은 복분자 추출물을 준비하였다.The same procedure as in Example 1 was performed, but 30% (v/v) alcohol was used as the extraction solvent, and a non-fermented bokbunja extract was prepared.
(비교예 3)(Comparative Example 3)
실시예1과 동일하게 실시하되, 추출 용매로 70%(v/v) 주정을 사용하고, 발효하지 않은 복분자 추출물을 준비하였다.The same procedure as in Example 1 was carried out, 70% (v/v) alcohol was used as the extraction solvent, and an unfermented bokbunja extract was prepared.
(Lactobacillus plantarum, 기탁번호: KACC91146)(L.P)Lactobacillus plant tarum
(Lactobacillus plantarum, accession number: KACC91146) (LP)
(Streptococcus thermophilus, 기탁번호: KACC91147)(S.th)Streptococcus thermophilus
(Streptococcus thermophilus, accession number: KACC91147) (S.th)
(Bifidobacterium longum, 기탁번호: KACC91563)(B.L)Bifidobacterium long sword
(Bifidobacterium longum, accession number: KACC91563)(BL)
(Lactobacillus helveticus, 기탁번호: KACC91148)(L.H)Lactobacillus Helvetica
(Lactobacillus helveticus, accession number: KACC91148)(LH)
(Enterococcus thailandicus, 기탁번호: KACC92253P)(E.th)Enterococcus Tyrandicus
(Enterococcus thailandicus, accession number: KACC92253P)(E.th)
(Lactobacillus plantarum, 기탁번호: KACC91146)(L.P)Lactobacillus plant tarum
(Lactobacillus plantarum, accession number: KACC91146) (LP)
(Streptococcus thermophilus, 기탁번호: KACC91147)(S.th)Streptococcus thermophilus
(Streptococcus thermophilus, accession number: KACC91147) (S.th)
(Bifidobacterium longum, 기탁번호: KACC91563)(B.L)Bifidobacterium long sword
(Bifidobacterium longum, accession number: KACC91563)(BL)
(Lactobacillus helveticus, 기탁번호: KACC91148)(L.H)Lactobacillus Helvetica
(Lactobacillus helveticus, accession number: KACC91148)(LH)
(Enterococcus thailandicus, 기탁번호: KACC92253P)(E.th)Enterococcus Tyrandicus
(Enterococcus thailandicus, accession number: KACC92253P)(E.th)
(Lactobacillus plantarum, 기탁번호: KACC91146)(L.P)Lactobacillus plant tarum
(Lactobacillus plantarum, accession number: KACC91146) (LP)
(Streptococcus thermophilus, 기탁번호: KACC91147)(S.th)Streptococcus thermophilus
(Streptococcus thermophilus, accession number: KACC91147) (S.th)
(Bifidobacterium longum, 기탁번호: KACC91563)(B.L)Bifidobacterium long sword
(Bifidobacterium longum, accession number: KACC91563)(BL)
(Lactobacillus helveticus, 기탁번호: KACC91148)(L.H)Lactobacillus Helvetica
(Lactobacillus helveticus, accession number: KACC91148)(LH)
(Enterococcus thailandicus, 기탁번호: KACC92253P)(E.th)Enterococcus Tyrandicus
(Enterococcus thailandicus, accession number: KACC92253P)(E.th)
(( 실험예1Experimental Example 1 ) ) DPPHDPPH 라디칼 소거활성 측정 Measurement of radical scavenging activity
실시예에서 제조한 발효 복분자 추출물, 및 비교예에서 제조한 복분자 추출물에 대하여 DPPH 라디칼 소거활성을 측정하였다.DPPH radical scavenging activity was measured for the fermented bokbunja extract prepared in Example and the bokbunja extract prepared in Comparative Example.
시료 10 mg을 DW 1 mL로 희석하여 0.1, 0.5, 1, 2, 3 mg/mL 농도로 각각 1 mL 씩 제조하였다. standard로 사용되는 BHT 용액은 BHT 10mg을 methanol 1mL로 희석하여 0.1, 0.5, 1, 2, 3 mg/mL의 농도로 각각 1 mL씩 제조하였다. DPPH 용액은 DPPH 0.002 g에 메탄올 25 mL를 혼합하여 제조하였다.10 mg of the sample was diluted with 1 mL of DW to prepare 1 mL each at a concentration of 0.1, 0.5, 1, 2, 3 mg/mL. The BHT solution used as a standard was prepared by diluting 10 mg of BHT with 1 mL of methanol and preparing 1 mL of each at a concentration of 0.1, 0.5, 1, 2, and 3 mg/mL. The DPPH solution was prepared by mixing 25 mL of methanol with 0.002 g of DPPH.
96 well plate에 BHT용액과 메탄올을 각각 80 mL씩 혼합한 군, BHT와 DPPH 용액을 각각 80 mL씩 혼합한 군, 시료와 DPPH 용액을 각각 80 mL씩 혼합한 군, 메탄올과 DPPH용액을 각각 80 mL씩 혼합한 군, 메탄올만을 160 mL씩 처리한 군, 증류수와 DPPH용액을 각각 80 mL씩 혼합한 군, 증류수와 메탄올을 각각 80 mL씩 혼합한 군을 각각 구분하여 분주한 후 빛과 반응하지 못하게 foil로 감싸준 뒤 실온에서 30분간 방치하였다. ELISA reader를 이용해 517㎚에서 흡광도를 측정하였다.A group of 80 mL of BHT solution and methanol each mixed in a 96 well plate, a group of 80 mL of BHT and DPPH solution each, a group of 80 mL of sample and DPPH solution each, and 80 mL of methanol and DPPH solution respectively. The group mixed with mL, the group treated with only 160 mL of methanol, the group mixed with 80 mL of distilled water and DPPH solution, and the group mixed with 80 mL of distilled water and methanol, respectively, were divided and dispensed. After wrapping with foil, it was left for 30 minutes at room temperature. Absorbance at 517 nm was measured using an ELISA reader.
흡광도 측정 후 동일한 추출 용매 및 동일한 추출물 농도에서 발효 복분자 추출물의 발효 전 대비 발효 후의 DPPH 라디칼 소거능 증가율을 하기 계산식 1로부터 계산하여, 그 결과를 도 1a 내지 도 1c에 도시하였다.After the absorbance measurement, the increase rate of DPPH radical scavenging ability after fermentation compared to before fermentation of the fermented bokbunja extract at the same extraction solvent and the same extract concentration was calculated from the following calculation formula 1, and the results are shown in FIGS. 1A to 1C.
[계산식 1] [Calculation formula 1]
상기 계산식 1에서, R, RA, RB는 각각 라디칼 소거능 증가율, 발효 복분자 추출물의 라디칼 소거능, 발효전 복분자 추출물의 라디칼 소거능이다.In the above formula 1, R, R A , R B are respectively the radical scavenging capacity increase rate, the radical scavenging ability of the fermented bokbunja extract, and the radical scavenging ability of the bokbunja extract before fermentation.
도 1a는 추출 용매로 물을 사용하여 추출된 복분자 추출물에 락토바실러스 플랜타럼(Lactobacillus plantarum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 비피도박테리움 롱검(Bifidobacterium longum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 및 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 균주를 접종하여 발효된 각각의 발효 복분자 추출물의 농도에 따른 DPPH 라디칼 소거능 증가율을 도시한 그래프이다.Figure 1a is Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus helveticus, Bifidobacterium longum, B. Streptococcus thermophilus) and Enterococcus thailandicus (Enterococcus thailandicus) is a graph showing the DPPH radical scavenging activity increase rate according to the concentration of each fermented bokbunja extract fermented by inoculation.
도 1a를 참조하면 추출 용매로 물을 사용하고, 발효 균주로 엔테로코커스 타이랜디커스(Enterococcus thailandicus)를 사용하여 발효시킨 발효 복분자 추출물은 40, 100, 200, 400 ㎍/ml의 농도에서 발효 전 복분자추출물보다 라디칼 소거능이 증가하였으며, 특히 200㎍/ml의 농도에서 라디칼 소거능 증가율이 가장 높은 것을 확인할 수 있다.Referring to Figure 1a, the fermented bokbunja extract fermented using water as an extraction solvent and Enterococcus thailandicus as a fermentation strain is a bokbunja before fermentation at a concentration of 40, 100, 200, 400 ㎍/ml It was confirmed that the radical scavenging activity increased compared to the extract, and the radical scavenging activity increase rate was particularly high at a concentration of 200 μg/ml.
또한 발효 균주로 스트렙코커스 써모필러스(Streptococcus thermophilus) 균주를 사용하여 발효시킨 발효 복분자 추출물은 40, 100, 200, 400 ㎍/ml의 농도에서 발효 전 복분자추출물보다 라디칼 소거능이 증가하였으며, 특히 200㎍/ml의 농도에서 라디칼 소거능 증가율이 가장 높은 것을 확인할 수 있다.In addition, the fermented bokbunja extract, fermented using the Streptococcus thermophilus strain as a fermentation strain, has increased radical scavenging ability than the bokbunja extract before fermentation at concentrations of 40, 100, 200, 400 ㎍/ml, especially 200 ㎍ It can be seen that at the concentration of /ml, the rate of radical scavenging activity was highest.
또한 발효 균주로 비피도박테리움 롱검(Bifidobacterium longum) 균주를 사용하여 발효시킨 발효 복분자 추출물은 100, 200, 400 ㎍/ml의 농도에서 발효 전 복분자추출물보다 라디칼 소거능이 증가하였으며, 특히 200㎍/ml의 농도에서 라디칼 소거능 증가율이 가장 높은 것을 확인할 수 있다.In addition, the fermented bokbunja extract, fermented using the Bifidobacterium longum strain as a fermentation strain, has increased radical scavenging ability than the bokbunja extract before fermentation at a concentration of 100, 200, 400 ㎍/ml, especially 200㎍/ml It can be seen that the highest rate of radical scavenging activity at the concentration of.
또한 발효 균주로 락토바실러스 플랜타럼(Lactobacillus plantarum) 균주를 사용하여 발효시킨 발효 복분자 추출물은 40, 100, 200, 400 ㎍/ml의 농도에서 발효 전 복분자추출물보다 라디칼 소거능이 증가하였으며, 특히 200㎍/ml의 농도에서 라디칼 소거능 증가율이 가장 높은 것을 확인할 수 있다.In addition, the fermented bokbunja extract, fermented using the Lactobacillus plantarum strain as a fermentation strain, has increased radical scavenging ability than the bokbunja extract before fermentation at concentrations of 40, 100, 200, 400 ㎍/ml, especially 200 ㎍/ It can be seen that at the concentration of ml, the rate of radical scavenging activity was highest.
또한 발효 균주로 락토바실러스 헬베티커스(Lactobacillus helveticus)를 사용하여 발효시킨 발효 복분자 추출물은 40, 100, 200, 400 ㎍/ml의 농도에서 50% 이상의 라디칼 소거능 증가율을 보였으며, 특히 200㎍/ml의 농도에서 라디칼 소거능 증가율이 가장 높은 것을 확인할 수 있다.In addition, the fermented bokbunja extract, fermented using Lactobacillus helveticus as a fermentation strain, showed a radical scavenging activity increase rate of 50% or more at a concentration of 40, 100, 200, 400 μg/ml, especially 200 μg/ml It can be seen that the highest rate of radical scavenging activity at the concentration of.
도 1b는 추출 용매로 30%(v/v) 주정을 사용하여 추출된 복분자 추출물에 락토바실러스 플랜타럼(Lactobacillus plantarum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 비피도박테리움 롱검(Bifidobacterium longum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 또는 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 균주를 접종하여 발효된 각각의 발효 복분자 추출물의 농도에 따른 DPPH 라디칼 소거능 증가율을 도시한 그래프이다.FIG. 1b shows Lactobacillus plantarum, Lactobacillus helveticus, Bifidobacterium longum on a bimodal extract extracted using 30% (v/v) alcohol as an extraction solvent. , Streptococcus thermophilus or Enterococcus thailandicus strain is a graph showing the increase rate of DPPH radical scavenging activity according to the concentration of each fermented bokbunja extract fermented by inoculation.
도 1b를 참조하면, 추출 용매로 30%(v/v) 주정을 사용하여 추출된 복분자 추출물을 이용하여 제조한 발효복분자추출물은 균주 농도에 관계없이 라디칼 소거능 증가율이 50%이상이었으며, 특히, 40㎍/ml의 농도에서 라디칼 소거능 증가율이 현격히 높은 것을 확인할 수 있다. Referring to FIG. 1B, the fermentation complex extracts prepared using the extracts of bokbunja using 30% (v/v) alcohol as an extraction solvent had a radical scavenging activity increase rate of 50% or more, regardless of strain concentration. It can be seen that at a concentration of µg/ml, the rate of radical scavenging activity is significantly higher.
발효복분자추출물의 농도가 40㎍/ml인 경우, 비피도박테리움 롱검(Bifidobacterium longum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 락토바실러스 플랜타럼(Lactobacillus plantarum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 및 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 균주를 각각 사용하여 제조한 항산화 조성물의 라디칼 소거능 증가율은 각각 1100%, 2920%, 2500%, 4040%, 2680%로 현격히 높게 측정되었다. 이를 통해, 복분자추출물 추출 시 추출 용매로 30%(v/v) 주정을 사용하고, 제조된 발효복분자추출물이 40㎍/ml의 농도로 항산화 조성물 내 포함될 경우 항산화 효과가 현격히 우수함을 확인할 수 있다.When the concentration of the fermentation complex extract is 40 μg/ml, Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus plantarum, Streptococcus thermophilus ) And Enterococcus thailandicus (Enterococcus thailandicus) strain, respectively, the radical scavenging activity increase rate of the antioxidant composition prepared was measured to be significantly higher, 1100%, 2920%, 2500%, 4040%, 2680%, respectively. Through this, 30% (v/v) alcohol is used as an extraction solvent when extracting a bi-molecular extract, and it can be confirmed that the antioxidant effect is remarkably excellent when the prepared fermented bi-molecular extract is included in the antioxidant composition at a concentration of 40 μg/ml.
도 1c는 추출 용매로 70%(v/v) 주정을 사용하여 추출된 복분자 추출물에 락토바실러스 플랜타럼(Lactobacillus plantarum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 비피도박테리움 롱검(Bifidobacterium longum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 또는 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 균주를 접종하여 발효된 각각의 발효 복분자 추출물의 농도에 따른 DPPH 라디칼 소거능 증가율을 도시한 그래프이다.Figure 1c is Lactobacillus plantarum, Lactobacillus helveticus, Bifidobacterium longum on the extract of bokbunja using 70% (v/v) alcohol as an extraction solvent. , Streptococcus thermophilus or Enterococcus thailandicus strain is a graph showing the increase rate of DPPH radical scavenging activity according to the concentration of each fermented bokbunja extract fermented by inoculation.
도 1c를 참조하면 추출 용매로 70%(v/v) 주정을 사용하고, 발효 균주로 엔테로코커스 타이랜디커스(Enterococcus thailandicus)를 사용하여 발효시킨 발효 복분자 추출물은 40, 100, 200, 400 ㎍/ml의 농도에서 각각 490, 953, 534, 240%의 라디칼 소거능 증가율을 나타내었으며, 100㎍/ml의 농도에서 라디칼 소거능 증가율이 가장 높은 것을 확인할 수 있다.Referring to Figure 1c, the fermentation bokbunja extract fermented using 70% (v/v) alcohol as an extraction solvent and Enterococcus thailandicus as a fermentation strain is 40, 100, 200, 400 μg/ It was found that the radical scavenging capacity increase rate was 490, 953, 534, and 240% at the concentration of ml, respectively, and it was confirmed that the radical scavenging capacity increase rate was the highest at the concentration of 100 μg/ml.
또한 발효 균주로 스트렙코커스 써모필러스(Streptococcus thermophilus) 균주를 사용하여 발효시킨 발효 복분자 추출물은 40, 100, 200, 400 ㎍/ml의 농도에서 각각 883, 1132, 610, 276%의 라디칼 소거능 증가율을 나타내었으며, 100㎍/ml의 농도에서 라디칼 소거능 증가율이 가장 높은 것을 확인할 수 있다.In addition, the fermented bokbunja extract, fermented using the Streptococcus thermophilus strain as a fermentation strain, increased the rate of radical scavenging activity of 883, 1132, 610, and 276% at concentrations of 40, 100, 200, and 400 μg/ml, respectively. It can be seen that, at a concentration of 100 μg/ml, the radical scavenging capacity increase rate was the highest.
또한 발효 균주로 비피도박테리움 롱검(Bifidobacterium longum) 균주를 사용하여 발효시킨 발효 복분자 추출물은 40, 100, 200, 400 ㎍/ml의 농도에서 각각 152, 295, 243, 101%의 라디칼 소거능 증가율을 나타내었으며, 100, 200㎍/ml의 농도에서 라디칼 소거능 증가율이 더욱 높은 것을 확인할 수 있다.In addition, the fermented bokbunja extract, fermented using the Bifidobacterium longum strain as a fermentation strain, increases the radical scavenging capacity increase rate of 152, 295, 243, and 101% at concentrations of 40, 100, 200, and 400 μg/ml, respectively. It can be seen that the radical scavenging capacity increase rate is higher at concentrations of 100 and 200 μg/ml.
또한 발효 균주로 락토바실러스 플랜타럼(Lactobacillus plantarum) 균주를 사용하여 발효시킨 발효 복분자 추출물은 40, 100, 200, 400 ㎍/ml의 농도에서 각각 1534, 1797, 961, 319%의 라디칼 소거능 증가율을 나타내었으며, 특히 40, 100㎍/ml의 농도에서 라디칼 소거능 증가율이 현저히 높은 것을 확인할 수 있다.In addition, the fermented bokbunja extract, fermented using the Lactobacillus plantarum strain as a fermentation strain, exhibits a radical scavenging activity increase rate of 1534, 1797, 961, and 319% at concentrations of 40, 100, 200, and 400 μg/ml, respectively. In particular, it can be seen that the radical scavenging capacity increase rate is remarkably high at concentrations of 40 and 100 μg/ml.
또한 발효 균주로 락토바실러스 헬베티커스(Lactobacillus helveticus)를 사용하여 발효시킨 발효 복분자 추출물은 40, 100, 200, 400 ㎍/ml의 농도에서 각각 807, 1363, 692, 319%의 라디칼 소거능 증가율을 나타내었으며, 100㎍/ml의 농도에서 라디칼 소거능 증가율이 가장 높은 것을 확인할 수 있다.In addition, the fermented bokbunja extract fermented using Lactobacillus helveticus as a fermentation strain exhibits an increase in radical scavenging ability of 807, 1363, 692, and 319% at concentrations of 40, 100, 200, and 400 μg/ml, respectively. It was confirmed that the radical scavenging capacity increase rate was the highest at a concentration of 100 μg/ml.
Claims (14)
Ronggeom Bifidobacterium (Bifidobacterium longum ), Lactobacillus helveticus , Lactobacillus plantarum , Streptococcus thermophilus and Enterococcus Thailandicus ) Antioxidant composition comprising a fermented bokbunja extract obtained by inoculating any one strain selected from the group consisting of bokbunja extract to fermentation.
상기 복분자 추출물은 추출 용매로 물을 이용하여 추출되고, 상기 발효복분자추출물은 상기 복분자 추출물에 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 또는 스트렙코커스 써모필러스(Streptococcus thermophilus) 균주를 접종하여 발효시킴으로써 수득된 것을 특징으로 하는 항산화 조성물.
According to claim 1,
The bokbunja extract is extracted using water as an extraction solvent, and the fermentation bokbunja extract is enterococcus ( Enterococcus) on the bokbunja extract. Thailandicus ) or Streptococcus thermophilus ( Streptococcus thermophilus ) antioxidant composition characterized in that obtained by inoculation and strain.
상기 발효복분자추출물은 170~450 ㎍/ml의 농도로 포함되는 항산화 조성물.
According to claim 2,
The fermentation complex extract is an antioxidant composition contained in a concentration of 170 ~ 450 ㎍ / ml.
상기 복분자 추출물은 추출 용매로 물을 이용하여 추출되고, 상기 발효복분자추출물은 상기 복분자 추출물에 비피도박테리움 롱검(Bifidobacterium longum) 균주를 접종하여 발효시킴으로써 수득된 것이고, 상기 조성물 내 170~250 ㎍/ml의 농도로 포함되는 것을 특징으로 하는 항산화 조성물.
According to claim 1,
The bokbunja extract is extracted using water as an extraction solvent, and the fermentation bokbunja extract is extracted from the bokbunja extract Bifidobacterium longum longum ) It is obtained by inoculating the strain and fermentation, the antioxidant composition characterized in that it is included in the concentration of 170 ~ 250 ㎍ / ml in the composition.
상기 복분자 추출물은 추출 용매로 물을 이용하여 추출되고, 상기 발효복분자추출물은 상기 복분자 추출물에 락토바실러스 플랜타럼(Lactobacillus plantarum) 균주를 접종하여 발효시킴으로써 수득된 것을 특징으로 하는 항산화 조성물.
According to claim 1,
The bokbunja extract is extracted using water as an extraction solvent, and the fermentation bokbunja extract is an antioxidant composition characterized in that obtained by inoculating the bokbunja extract Lactobacillus plant tarum ( Lactobacillus plantarum ) strain.
상기 발효복분자추출물은 170~450 ㎍/ml의 농도로 포함되는 항산화 조성물.
The method of claim 5,
The fermentation complex extract is an antioxidant composition contained in a concentration of 170 ~ 450 ㎍ / ml.
상기 복분자 추출물은 추출 용매로 20~40%(v/v) 주정을 이용하여 추출되는 것을 특징으로 하는 항산화 조성물.
According to claim 1,
Antioxidant composition, characterized in that the bokbunja extract is extracted using 20-40% (v/v) alcohol as an extraction solvent.
상기 발효복분자추출물은 20~250 ㎍/ml의 농도로 포함되는 항산화 조성물.
The method of claim 7,
The fermentation complex extract is an antioxidant composition contained in a concentration of 20 ~ 250 ㎍ / ml.
상기 복분자 추출물은 추출 용매로 60~80%(v/v) 주정을 이용하여 추출되는 것을 특징으로 하는 항산화 조성물.
According to claim 1,
Antioxidant composition, characterized in that the extract of bokbunja is extracted using 60-80% (v/v) alcohol as an extraction solvent.
상기 발효복분자추출물은 상기 복분자 추출물에 비피도박테리움 롱검(Bifidobacterium longum) 균주를 접종하여 발효시킴으로써 수득된 것이고, 상기 조성물 내 80~250 ㎍/ml의 농도로 포함되는 것을 특징으로 하는 항산화 조성물.
The method of claim 9,
The fermented bokbunja extract is Bifidobacterium long gum in the bokbunja extract longum ) It is obtained by inoculating the strain and fermentation, the antioxidant composition characterized in that it is included in the concentration of 80 ~ 250 ㎍ / ml in the composition.
상기 발효복분자추출물은 상기 복분자 추출물에 락토바실러스 헬베티커스(Lactobacillus helveticus), 락토바실러스 플랜타럼(Lactobacillus plantarum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 및 엔테로코커스 타이랜디커스(Enterococcus thailandicus)으로 이루어진 군에서 선택된 어느 하나의 균주를 접종하여 발효시킴으로써 수득된 것이고, 상기 조성물 내 20~250 ㎍/ml의 농도로 포함되는 것을 특징으로 하는 항산화 조성물.
The method of claim 9,
The fermentation extract was bokbunja Lactobacillus helveticus carcass (Lactobacillus helveticus), Lactobacillus plan tareom (Lactobacillus plantarum), streptavidin Lactococcus Thermo pillar's (Streptococcus thermophilus) and Enterococcus tie Randy carcass (Enterococcus the bokbunja extract Thailandicus ) is obtained by inoculating any one strain selected from the group consisting of, and the antioxidant composition, characterized in that contained in a concentration of 20 ~ 250 ㎍ / ml in the composition.
상기 비피도박테리움 롱검(Bifidobacterium longum), 락토바실러스 헬베티커스(Lactobacillus helveticus), 락토바실러스 플랜타럼(Lactobacillus plantarum), 스트렙코커스 써모필러스(Streptococcus thermophilus) 및 엔테로코커스 타이랜디커스(Enterococcus thailandicus) 균주의 기탁번호는 각각 KACC91563, KACC91148, KACC91146, KACC91147 및 KACC92253P인 것을 특징으로 하는 항산화 조성물.
According to claim 1,
The ronggeom Bifidobacterium (Bifidobacterium longum ), Lactobacillus helveticus , Lactobacillus plantarum , Streptococcus thermophilus and Enterococcus thailandicus ) The deposit number of the strain is KACC91563, KACC91148, KACC91146, KACC91147 and KACC92253P, respectively, antioxidant composition.
Health functional food for antioxidant, comprising the antioxidant composition according to claim 1.
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| JP2006008566A (en) * | 2004-06-24 | 2006-01-12 | Ichimaru Pharcos Co Ltd | Cosmetic agent containing lactic acid bacteria fermentation product of fruit juice as active ingredient and its application |
| KR20080094459A (en) * | 2007-04-20 | 2008-10-23 | 주식회사 엘지생활건강 | Cosmetic composition having antioxidation activity |
| KR101365917B1 (en) * | 2011-06-29 | 2014-02-20 | 전라북도 고창군 | Making method for fermentation beverage of rubus coreanus |
| KR20180056455A (en) * | 2016-11-18 | 2018-05-29 | 대한민국(농촌진흥청장) | Manufacturing method of berries fermented liquid using lactic acid bacteria and berries fermented liquid manufactured by same method |
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| JP2006008566A (en) * | 2004-06-24 | 2006-01-12 | Ichimaru Pharcos Co Ltd | Cosmetic agent containing lactic acid bacteria fermentation product of fruit juice as active ingredient and its application |
| KR20080094459A (en) * | 2007-04-20 | 2008-10-23 | 주식회사 엘지생활건강 | Cosmetic composition having antioxidation activity |
| KR101365917B1 (en) * | 2011-06-29 | 2014-02-20 | 전라북도 고창군 | Making method for fermentation beverage of rubus coreanus |
| KR20180056455A (en) * | 2016-11-18 | 2018-05-29 | 대한민국(농촌진흥청장) | Manufacturing method of berries fermented liquid using lactic acid bacteria and berries fermented liquid manufactured by same method |
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