KR20190053475A - A composition for rice bacterial blight suppressing activity - Google Patents
A composition for rice bacterial blight suppressing activity Download PDFInfo
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Abstract
Description
본 발명은 스트렙토마이세스 MJM8519(Streptomyces MJM8519) 균주로부터 생산되는 Venturicidin B을 포함하는 벼흰잎마름병 방제용 조성물에 관한 것이다. The present invention relates to a composition for the control of rice blight huinip containing Venturicidin B produced from Streptomyces MJM8519 (Streptomyces MJM8519) strain.
벼흰잎마름병의 병징은 묘판 후기에 아래 잎의 가장자리에 물에 젖은 듯한 작은 병반이 나타나며, 그 표면에 분출된 세균의 마른 딱지가 형성된다. 발병된 지 며칠이 지나면 병반이 노란색으로 변하게 되고, 잎의 끝부분부터 하얗게 마르면서 잎이 죽게 된다. 감수성이 매우 높은 품종에서는 묘를 이앙한 후부터 분얼 최성기까지의 벼가 갑자기 말라죽는 급성형 병징(kresek)이 있는데 잎에서는 뚜렷한 병징이 없고, 잎의 가장자리나 잎 끝에 좁쌀보다 작은 세균의 마른 딱지가 보일 뿐이다. 분얼 최성기 이후 성숙된 잎에 나타난 병증상은 5∼6cm 아래의 잎 가장자리에 물에 젖은 듯한 작은 무늬가 생긴다. 아침 이슬이 맺힐 때 이 병반의 표면에는 옅은 우유빛의 이슬이 맺히며, 이것이 말라서 황색의 딱지로 남아 있는 것을 볼 수 있다. 7∼13일간 병이 진전되면 병반이 커지면서 황색∼등황색이 되고, 건강한 잎조직과의 경계는 물에 젖은 듯한 수침상으로 된다. 병반은 잎의 한쪽 또는 양쪽 가장자리에 형성되어 진전되며, 후에 잎 중앙맥까지 번지며 물결 모양으로 확대된다. 병이 더 진전되면 황백색 병반이 백색∼회백색으로 변하고, 부생균에 의해 오염되면서 더러운 색깔로 변한다.The symptom of rice blight blight appears in the late stage of the trunk after the bottom edge of the leaf, which appears to be wet with water, and a dry scab of the germ emerged on the surface. After a few days after the onset of the disease, the lesion turns yellow and the leaf is dead as it dries white from the tip of the leaf. In highly susceptible varieties, there is a kresek in which the rice from sudden growth of the seedlings to the end of bloom suddenly dies. There is no obvious symptom in the leaves, and there is a dry scab at the edge of the leaf, Only. The disease symptoms that appear on mature leaves after the bloom peak season are small patterns that appear to be wet with water on the edge of the leaf under 5 to 6 cm. When the morning dew grows, the surface of this lesion has a dew of light milk, which can be seen to remain yellow as a dry scab. When the disease develops for 7 to 13 days, the lesion grows from yellow to light yellow, and the boundary with healthy leaf tissue becomes watery, like water. The lesion is formed on one or both edges of the leaf and then spreads to the leaf central vein and expands in a wavy pattern. As the disease progresses, the yellowish white lesion turns white to grayish white, becomes dirty by contamination by the negative bacteria.
벼흰잎마름병원균인 Xanthomonas oryzae pv . Oryzae는 그람음성의 호기성 세균으로서 단극모를 가지고 있고, 막대모양이다. 고체배지 상에서 황색의 집락(colony)을 형성하며, 자라는 속도는 다소 느리고, 생육 최적온도는 26∼30℃이다. 병반에 말라붙은 세균덩어리나 병들어 죽은 식물체 속에 남아 있는 병원세균은 8개월 이상 살 수 있으나, 고온 ·다습한 환경에서는 짧은 시일 내에 죽는다. Xanthomonas oryzae pv . Oryzae is a Gram-negative, aerobic bacterium that has unipolar hairs and is rod-shaped. It forms a yellow colony on the solid medium, the growth rate is somewhat slow, and the optimal growth temperature is 26 to 30 ° C. Bacteria dried in diseased lesions or diseased Bacteria remaining in dead plants can live for more than 8 months, but die quickly in high temperature and high humidity environments.
발병을 일으키는 생태는 병원세균은 수로나 저수지에 흔히 볼 수 있는 잡초 중에 겨풀, 나도겨풀의 근권이나 병든 볏짚에서 월동한다. 월동한 병원세균들이 1차전염원이 되어 논물에 유입된 후 수면에 접촉된 벼잎의 배수선이나 기공으로 침입하여 병반을 형성하게 된다. 병반에서 증식된 병원세균이 2차 전염원이 되어 논물 속으로 배출되거나 직접 병반과 건전한 벼 조직의 접촉에 의하여 전염된다. 강한 바람은 벼에 상처를 주어 병원세균이 침입하기 쉽게 하고, 병반부에서 누출된 병원세균을 바람에 실어 50m 이상 운반한다. 또한 태풍에 동반된 많은 비로 인해 벼가 물에 잠기게 되면 배수선이나 기공을 통한 병원세균의 침입이 더욱 쉬워진다. 7월 평균기온이 22∼24℃로 저온일 때, 7월의 강수량이 200mm 이상으로 많은 비가 올 때, 15m/sec 이상의 강풍이 불 때 이 병의 발생이 특히 많아진다. 모판이나 본답에서 질소비료를 많이 주게 되면 벼가 감수성으로 되어 이 병의 발병을 촉진시킨다 The ecology that causes the disease is that the pathogenic germs winter in the weeds, which are common in waterways and reservoirs. In winter, the pathogenic bacterium enters into the first source and enter into the soil. The pathogenic bacterium propagated in the lesion becomes a secondary infectious source and is released into the soil or directly by the contact between the lesion and healthy rice tissue. Strong winds cause rice bruises, which make hospital bugs more susceptible to infiltration, and carry more than 50 meters of windborne pathogens from the lesions. In addition, when the rice is submerged in water due to the heavy rainfall accompanying the typhoon, the invasion of the pathogenic bacteria through the water line or pore becomes easier. When the average temperature in July is low at 22 ~ 24 ℃, rainfall in July is more than 200mm and rain is more than 15m / sec. If you give plenty of nitrogen fertilizer in the plate or in this answer, the rice becomes susceptible and promotes the onset of this disease
그리고 스트렙토마이세스와 같은 방선균으로부터 이미 다양한 항생물질이 분리되었으나, 현재 상기 항생물질이 정확히 어떠한 미생물에 대하여 항생효과를 나타내는지에 대해서 연구가 진행중에 있다.In addition, various antibiotics have already been isolated from actinomycetes such as streptomycetes, but currently research is underway to determine exactly which antibiotics exhibit antibiotic effect against the microorganisms.
본 발명자들은 방선균으로부터 분리한 항생물질인 Venturicidin B가 그람 음성 균주인 벼흰잎마름병의 원인 균주에 대한 억제 효과를 확인함으로써 본 발명을 완성하였다.The present inventors have completed the present invention by confirming the inhibitory effect of Venturicidin B, an antibiotic substance isolated from actinomycetes, against a causative strain of Gram-negative blight of rice blight.
본 발명은 벼흰잎마름병의 활성을 억제하는 벼흰잎마름병 방제용 조성물을 제공하고자 한다.The present invention is to provide a composition for controlling blight of blight of blight of white blossom that inhibits the activity of blight of blight of blight.
본 발명은 Venturicidin B를 포함하는 벼흰잎마름병 방제용 조성물을 제공한다.The present invention provides a composition for preventing blight of rice blotch blight comprising Venturicidin B.
또한 본 발명은 (a) 수탁번호 KACC92147P로 기탁된 스트렙토마이세스 MJM8519 균주를 배양하는 단계; (b)상기 배양물을 유기용매로 추출하는 단계; (c)상기 추출물에 대해 크로마토그래피를 수행하여 활성분획을 수득하는 단계; (d)상기 활성분획을 정제하는 단계를 포함하는 Venturicidin B의 제조방법을 제공한다.(A) culturing a Streptomyces strain MJM8519 deposited with accession number KACC92147P; (b) extracting the culture with an organic solvent; (c) subjecting the extract to a chromatography to obtain an active fraction; (d) purifying the active fraction.
또한 본 발명은 상기 방제용 조성물을 벼, 벼의 종자 또는 벼의 재배 토양에 처리하는 것을 특징으로 하는 벼흰잎마름병 방제 방법을 제공한다.The present invention also provides a method for controlling blight of rice blast fungus, which comprises treating the composition for controlling rice with rice, rice seeds or cultivated soil of rice.
본 발명에 따른 벼흰잎마름병 방제용 조성물은 세계 3대 식물 중 하나인 벼의 가장 큰 피해를 야기하는 벼흰잎마름병을 억제하여 벼 재배 지역의 피해를 최소화할 수 있다.The composition for controlling blight of rice blight according to the present invention can suppress the blight of blight of blight of rice which causes the greatest damage of rice, which is one of the three largest plants in the world, and can minimize the damage to the rice cultivation area.
도 1은 스트렙토마이세스 MJM8519 균주에서 유래된 Venturicidin B의 화학식이다.
도 2는 스트렙토마이세스 MJM8519 균주에서 유래된 Venturicidin B를 RP-HPLC 분리 후, UPLC-Q-TOF/MS 및 UHPLC-LTQ-IT-MS/MS로 분석한 결과를 나타낸 크로마토그램 피크 패턴이다.
도 3은 스트렙토마이세스 MJM8519 균주에서 유래된 Venturicidin B 및 DAPG(2,4-diacetylphloroglucinol)의 벼잎생엽절편 스크리닝 결과를 나타낸 그래프이다; 1컬럼: 음성대조군, 2-11컬럼: HPLC 분획, 12컬럼: 양성대조군(DAPG, 2,4-Diacetylphloroglucinol). row 1 내지 8은 HPLC 분획을 각각 희석한 것(row 1 = 1/2, row 2 = 1/4, row 3 = 1/8, row 4 = 1/16, row 5 = 1/32, row 6 = 1/64, row 7 = 1/128, row 8 = 1/256).Figure 1 shows the formula of Venturicidin B derived from Streptomyces MJM8519 strain.
FIG. 2 is a chromatogram peak pattern showing the result of analysis of UPPLC-Q-TOF / MS and UHPLC-LTQ-IT-MS / MS after RP-HPLC separation of Venturicidin B derived from Streptomyces MJM8519 strain.
FIG. 3 is a graph showing the results of screening of leaf leaf slices of Venturicidin B and DAPG (2,4-diacetylphloroglucinol) derived from Streptomyces MJM8519 strain; 1 column: negative control, 2-11 column: HPLC fraction, 12 column: positive control (DAPG, 2,4-Diacetylphloroglucinol). Rows 1 to 8 are diluted HPLC fractions (row 1 = 1/2,
본 발명은 화학식Ⅰ의 Venturicidin B를 포함하는 벼흰잎마름병 방제용 조성물을 제공한다.The present invention provides a composition for preventing blight of rice blotch blight comprising Venturicidin B of Chemical Formula (I).
본 발명은 상기 Venturicidin B가 수탁번호 KACC92147P로 기탁된 스트렙토마이세스 MJM8519(streptomyces MJM8519) 균주에서 유래된 것을 특징으로하는 벼흰잎마름병 방제용 조성물을 제공한다.The present invention is the Streptococcus Venturicidin B deposited as accession No. KACC92147P My process MJM8519 (streptomyces The present invention provides a composition for controlling blight of blight of rice blast, which is derived from a strain of MJM8519.
또한 본 발명은 상기 벼흰잎마름병이 잔토모나스 속 세균에 의해 발생되는 식물병임을 특징으로하는 벼흰잎마름병 방제용 조성물을 제공한다.The present invention also provides a composition for controlling blight of blight of white blossom, wherein the blight of blight is caused by a bacterium belonging to the genus Zanthomonas.
본 발명에 따른 한 구체예에서, 스트렙토마이세스 MJM8519 유래 Venturicidin B가 벼흰잎마름병을 유발하는 병원균 Xanthomonas oryzae pv . Oryzae에 대한 우수한 항균활성을 가진다는 것이 본 발명자들에 의하여 확인되었다.In one embodiment according to the present invention, Venturicidin B from Streptomyces MJM8519 is a pathogen Xanthomonas oryzae pv . It has been confirmed by the present inventors that it has an excellent antibacterial activity against Oryzae .
특히, 식물병원체에 대한 억제 활성이 알려진 자연 페놀 화합물인 Pseudomonas fluorescens 그람 음성 박테리움 DAPG(2,4-diacetylphloroglucinol)와 유사한 벼희잎마름병 병원균에 대한 저항활성을 보임을 실험적으로 밝혔다(실시예 3)In particular, it has been experimentally demonstrated that Pseudomonas fluorescens , which is a natural phenolic compound having inhibitory activity against plant pathogens, exhibits a resistance activity against a pathogenic fungus such as DAPG (2,4-diacetylphloroglucinol) (Example 3)
본 발명은 (a) 수탁번호 KACC92147P로 기탁된 스트렙토마이세스 MJM8519(Streptomyces MJM8519) 균주를 배양하는 단계; (b)상기 배양물을 유기용매로 추출하는 단계; (c)상기 추출물에 대해 크로마토그래피를 수행하여 활성분획을 수득하는 단계; (d)상기 활성분획을 정제하는 단계를 포함하는 Venturicidin B의 제조방법을 제공한다.The present invention comprises the steps of culturing the Streptomyces MJM8519 (Streptomyces MJM8519) strain deposited as (a) accession No. KACC92147P; (b) extracting the culture with an organic solvent; (c) subjecting the extract to a chromatography to obtain an active fraction; (d) purifying the active fraction.
상기 활성분획을 수득하는 단계 후에, (e) 상기 수집된 물질에 대해 질량분석을 수행하여 Venturicidin B를 확인 하는 단계를 더 포함할 수 있다.(E) subjecting the collected material to mass spectrometry to identify Venturicidin B after the step of obtaining the active fraction.
상기 유기용매는 메탄올, 에탄올, 부탄올, 주정, 아세톤, 아세트산 에틸, 에테르, 클로로포름, 펜탄, 헥산, 헵탄, 노난, 데칸, 벤젠, 톨루엔 및 자이렌으로 이루어진 군에서 선택되는 어느 하나 이상인 것일 수 있으나, 이에 한정되지 않는다. 여기서 '주정'이란 전분질 원료 또는 당질 원료를 발효시킨 후 증류하여 만든 에탄올을 의미한다. 상기 유기용매로써는 아세트산 에틸, 메탄올, 에탄올, 부탄올 및 주정 등 알코올인 것이 바람직하고, 아세트산 에틸인 것이 가장 바람직하나, 이에 한정되지 않는다. The organic solvent may be any one or more selected from the group consisting of methanol, ethanol, butanol, ethanol, acetone, ethyl acetate, ether, chloroform, pentane, hexane, heptane, nonane, decane, benzene, toluene, But is not limited thereto. Herein, the term " alcohol " means ethanol produced by fermenting a starch raw material or a saccharide raw material and then distilling it. The organic solvent is preferably an alcohol such as ethyl acetate, methanol, ethanol, butanol and alcohol, most preferably ethyl acetate, but is not limited thereto.
상기 크로마토그래피는 이에 한정되는 것은 아니나, 흡착제로서 실리카겔, 역상실리카겔, 세파덱스류, 다이아이온 레진류, 알루미나, 활성탄, 규산마그네슘, 탄산칼슘, 인산칼슘, 인산마그네슘, 마그네시아 또는 수산화칼륨 등을 사용할 수 있으며, 세파덱스류를 사용할 수 있다. 보다 구체적으로는, 세파덱스 컬럼 크로마토그래피를 수행한 후 고성능 액체 크로마토그래피를 한번 더 수행할 수 있다.As the adsorbent, silica gel, reverse phase silica gel, Sephadex, Diaion resins, alumina, activated carbon, magnesium silicate, calcium carbonate, calcium phosphate, magnesium phosphate, magnesia or potassium hydroxide can be used as the adsorbent And Sephadexes can be used. More specifically, high performance liquid chromatography can be performed once more after performing Sephadex column chromatography.
본 발명에 따른 Venturicidin B를 생산하는 스트렙토마이세스 MJM8519균주 또는 그 배양 여액을 포함하는 벼흰잎마름병 방제용 조성물을 제공한다. A Streptomyces strain MJM8519 producing Venturicidin B according to the present invention or a culturing filtrate thereof.
상기 Venturicidin B를 유효성분으로 포함하여 벼흰잎마름병 방제용 살균제를 제조하기 위하여 본 발명이 속하는 기술분야에서 통상적으로 사용되는 충전제, 중량제, 계면활성제 등을 혼합하여 벼흰잎마름병 방제용 조성물을 제조하는 것이 가능하다.In order to prepare a bactericide for preventing blight of blight of white blossom with Venturicidin B as an active ingredient, a composition for controlling bacterial blight of blight of white blossom is prepared by mixing filler, weight agent, surfactant, etc. commonly used in the technical field of the present invention It is possible.
본 발명에 따른 방제용 조성물을 벼, 벼의 종자 또는 벼의 재배 토양에 처리하는 것을 특징으로 하는 벼흰잎마름병 방제 방법을 제공한다.The present invention also provides a method for controlling blight of rice blast fungus, which comprises treating a control composition according to the present invention with rice, rice seeds or cultivated soil of rice.
이하 본 발명에 따르는 실시예 및 본 발명에 따르지 않는 비교예를 통하여 본 발명을 보다 상세히 설명하나, 본 발명의 범위가 하기 제시된 실시예에 의해 제한되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to the following examples and comparative examples, but the scope of the present invention is not limited by the following examples.
<< 실시예Example 1> 1> 벼흰잎마름병에In rice blight blight 항균 활성을 갖는 Having antimicrobial activity 스트렙토마이세스Streptomyces MJM8519MJM8519 균주의 분리 및 동정 Isolation and Identification of Strain
스트렙토마이세스(Streptomyces sp .)를 분리하기 위하여, 함박산(경기, 한국)에서 얻은 1g의 토양 샘플을 세척하여 60℃에서 30분 동안 가열하고, 스트렙토마이세스를 분리하기 위해 가열된 샘플을 HV(Humic acid vitamin) 한천 배지로 옮겼다. Streptomyces (Streptomyces sp . 1 g of a soil sample obtained from Mt. Hambuk (Gyeonggi, Korea) was washed and heated at 60 DEG C for 30 minutes. To remove the streptomyces , the heated sample was suspended in Humic acid vitamin agar medium I moved.
한천에서 자란 단일 콜로니를 분리하여 GSS 배지(가용 녹말 1%, 글루코스 2%, 대두박 2.5%, 쇠고기 추출물 0.1%, 효모 추출물 0.4%, NaCl 0.2%, K2HPO4 0.025%, CaCO3 0.2%, pH 7.2)에서 200rpm, 28℃에서 7일 간 배양하고 16개의 2L 삼각 플라스크에 650 ml씩 나누어 담았다.Single colonies grown in agar were separated and cultured in GSS medium (1% of soluble starch, 2% of glucose, 2.5% of soybean meal, 0.1% of beef extract, 0.4% of yeast extract, 0.2% of NaCl, 0.025% of K 2 HPO 4 , 7.2) for 7 days at 200 rpm and 28 ° C, and were divided into 16 2L Erlenmeyer flasks in 650 ml portions.
스트렙토마이세스를 동정하기 위하여, 전장 16S rDNA를 유니버셜 프라이머 세트(universal primer set)인 27F 프라이머(5'-AGA GTT TGA TCA TGG CTC AG-3')(서열번호 1) 및 1492R 프라이머(5'-GGA TAC CTT GTT ACG ACT T-3')(서열번호 2)를 이용하여 증폭하였고, 상기 서열을 NCBI 홈페이지(National Center for Biotechnology Information; http://www.ncbi.nlm.nih.gov/)에서 분석하였다. In order to identify streptomyces , full-length 16S rDNA was amplified by PCR using a universal primer set 27F primer (5'-AGA GTT TGA TCA TGG CTC AG-3 ') (SEQ ID NO: 1) and 1492R primer GGA TAC CTT GTT ACG ACT T-3 ') (SEQ ID NO: 2), and the sequence was amplified from the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/) Respectively.
그 결과 분리된 균주는 스트렙토마이세스 속 균주와 99%의 상동성을 가짐을 확인하였다. 따라서 분리된 스트렙토마이세스 균주를 스트렙토마이세스 MJM8519로 명명하였으며, 2016년 10월 13일자로 KACC에 기탁하여 수탁번호 KACC92147P를 부여 받았다.As a result, it was confirmed that the isolated strain had 99% homology with Streptomyces sp . Thus, the isolated Streptomyces strain was designated as Streptomyces MJM8519 and deposited with KACC on Oct. 13, 2016, and received the accession number KACC92147P.
<< 실시예Example 2> 2> 스트렙토마이세스Streptomyces MJM8519MJM8519 균주로부터 From strain 벼흰잎마름병에In rice blight blight 항균 활성을 갖는 Venturicidin B의 분리 및 동정 Isolation and Identification of Venturicidin B with Antimicrobial Activity
벼흰잎마름병 억제 활성 화합물을 분리하기 위하여, 스트렙토마이세스 MJM8519 균주를 실시예 1에 따른 GSS 배지에서 28일 간 배양한 후 배양액을 제거하고 균체에 80% 메탄올을 첨가하여 공지의 방법으로 마쇄하였다. 상기 마쇄한 균개에 에틸아세테이트 300ml를 첨가하고 4℃에서 10분간 15,000rpm으로 원심분리 한 후, SepPak SPE(고체상 추출, Solid phase extraction) 시료를 이용한 세파덱스(Sephadex) LH-20 컬럼 크로마토그래피로 분획하였다. 이후, C18 역상 고성능 액체 크로마토그래피(reverse-phase high-performance liquid chromatography, C18 RP-HPLC)로 활성 화합물을 분리하였으며, HPLC의 20번째 분획에서 활성 화합물의 함량이 가장 높은 것을 확인하였다. Streptomyces MJM8519 strain was cultivated for 28 days in the GSS medium according to Example 1, and then the culture broth was removed and 80% methanol was added to the cells to be polished by a known method. 300 ml of ethyl acetate was added to the calcined fungus, and the mixture was centrifuged at 15,000 rpm for 10 minutes at 4 ° C. The fractions were fractionated by Sephadex LH-20 column chromatography using a SepPak SPE (solid phase extraction) Respectively. Since, C 18 reverse phase high performance liquid chromatography (reverse-phase high-performance liquid chromatography, C 18 RP-HPLC) was separated into the active compound, it was confirmed that from the 20 th fraction of the HPLC with the highest content of the active compound.
수득한 활성 화합물을 UPLC-Q-TOF/MS(ultra-performance liquid chromatography quadrupole time of flight mass spectrometry)로 분석한 결과, 분자량은 706.47 m/z, 분자식은 C40H66O10 이었다(도 2). Analysis of the resulting active compound by UPLC-Q-TOF / MS showed that the molecular weight was 706.47 m / z and the molecular formula was C 40 H 66 O 10 (FIG. 2) .
<< 실시예Example 3> 3> 스트렙토마이세스Streptomyces MJM8519MJM8519 균주 유래 Strain derived VenturicidinVenturicidin B의 Of B 벼흰잎마름병에In rice blight blight 대한 억제 활성 Inhibitory activity against
Venturicidin B의 벼흰잎마름병에 대한 억제 활성을 확인하기 위하여, 96웰 플레이트에서 2배 연속 희석법을 수행하였다. 벼흰잎마름병의 병원균인 Xanthomonas oryzae pv . Oryzae KACC 10331의 배양 배지를 벼잎생엽절편에 투여하였다. 벼잎생엽절편의 병원균 감염 정도는 육안으로 확인하였다.In order to confirm the inhibitory activity of Venturicidin B against rice blast fungus, two consecutive serial dilutions were carried out in a 96 well plate. Xanthomonas oryzae , a pathogen of rice blight blight disease pv . The culture medium of Oryzae KACC 10331 was applied to the leaf leaf slices of the rye leaves. The degree of pathogen infection of the leaf biopsy of the rye leaf was visually confirmed.
그 결과, Venturicidin B에 해당하는 HPLC 분획을 투여한 96웰 플레이트 중 2컬럼 내 벼잎생엽절편이 황색이 아닌 녹색을 나타내어, 양성대조군인 DAPG(2,4-Diacetylphloroglucinol)과 같은 병원균 감염 억제 효과가 있음을 확인하였다(도 3).As a result, the green leaf of the leaf of the rye leaf in the two columns of the 96-well plate administered with the HPLC fraction corresponding to Venturicidin B exhibited a non-yellow green color, and the inhibitory effect on the pathogen infection such as the positive control DAPG (2,4-Diacetylphloroglucinol) (Fig. 3).
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Claims (6)
[화학식Ⅰ]
(I)
상기 Venturicidin B는 수탁번호 KACC92147P로 기탁된 스트렙토마이세스 MJM8519 (Streptomyces MJM8519) 균주에서 유래된 것인 벼흰잎마름병 방제용 조성물.The method according to claim 1,
The Venturicidin B is a Streptomyces deposited as accession No. KACC92147P MJM8519 (Streptomyces 0.0 > MJM8519). ≪ / RTI >
상기 벼흰잎마름병은 잔토모나스(Xanthomonas) 속 세균에 의해 발생되는 식물병임을 특징으로하는 벼흰잎마름병 방제용 조성물.The method according to claim 1,
Wherein the blight of rice blight is a plant disease caused by a bacterium belonging to the genus Xanthomonas .
(b)상기 배양물을 유기용매로 추출하는 단계; (c)상기 추출물에 대해 크로마토그래피를 수행하여 활성분획을 수득하는 단계; 및
(d)상기 활성분획을 정제하는 단계;를 포함하는 Venturicidin B의 제조방법.(a) Streptomyces MJM8519 deposited with Accession No. KACC92147P ( Streptomyces MJM8519);
(b) extracting the culture with an organic solvent; (c) subjecting the extract to a chromatography to obtain an active fraction; And
(d) purifying the active fraction.
상기 단계 (b)의 유기용매는 아세트산 에틸(ethyl acetate)인 것인, Antibiotic FD893의 제조방법.5. The method of claim 4,
Wherein the organic solvent in step (b) is ethyl acetate.
A method for controlling blight of rice blast fungus, which comprises treating the composition for controlling rice as set forth in any one of claims 1 to 3 to a cultivated soil of rice, rice seed or rice.
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| US3636198A (en) * | 1967-06-30 | 1972-01-18 | Ciba Geigy Corp | Venturicidin b and x and process for their manufacture |
| KR20130075026A (en) | 2011-12-27 | 2013-07-05 | 대한민국(농촌진흥청장) | Composition for rice bacterial blight suppressing activity |
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| US3636198A (en) * | 1967-06-30 | 1972-01-18 | Ciba Geigy Corp | Venturicidin b and x and process for their manufacture |
| KR20130075026A (en) | 2011-12-27 | 2013-07-05 | 대한민국(농촌진흥청장) | Composition for rice bacterial blight suppressing activity |
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