KR20170138822A - A cosmetic composition for skin whitening - Google Patents

Abstract

More particularly, the present invention relates to a cosmetic composition for whitening skin, which comprises 69.150 wt% of water, 15.000 wt% of glycerin, 0.300 wt% of a thickener, 0.200 wt% of a skin elasticity agent, 0.500 wt% of hyaluronic acid, 1.000wt%, vitamin C 0.500wt%, skin conditioning agent 0.100wt%, mushroom extract 2.000wt%, shiitake mushroom extract 2.000wt%, rice flour 2.000wt%, ASTRIN-AG 2.000wt% , 0.0020 wt% of essential oil, and 0.002 wt% of orange extract, and 0.2 wt% of solubilizing agent, 3.000 wt% of ethanol, 0.020 wt% of FRANKINCENSE, 0.020 wt% of PATCHOULI extract,

Description

A cosmetic composition for skin whitening < RTI ID = 0.0 >

The present invention relates to a cosmetic composition for skin whitening, and more particularly, to a cosmetic composition for skin whitening, which can inhibit tyrosinase, an important enzyme in the biosynthesis of melanin, thereby reducing the production of melanin, To a cosmetic composition for skin whitening.

Melanin is a biopolymer of phenols widely distributed in the natural world. It is a black pigment and protein complex. Melanin synthesis around us is a phenomenon that occurs when the cut surface of apples, potatoes and bananas is exposed to air, Feathers, skin, hair, eyes and the like. The function of melanin increases the viability of microorganisms in ultraviolet irradiation, propagation, drying, and extreme temperatures. In animals, skin and hair melanin function as a means of self-protection.

In addition, melanin present in invertebrates and fungi acts as an immune system for microorganisms and contributes to the quality of coffee, tea, tobacco, etc. (see J. Amm. Rev. Phytophathol. 240, 411. 1986) . However, human pigmentation and skin pigmentation, such as freckles, are caused by the ideal increase in melanin pigment in the epidermis, and melanin is biosynthesized in melanocytes in pigmented cells called melanocytes in the epidermal basal layer.

The main production process of melanin is the tyrosine tyrosinase, which is a kind of amino acid, which is oxidized to dopa and dopaquinone. These enzymes are metabolized by dopa chromium, indole carboxylic acids, indolequinones, Melanin is synthesized (see J. Mutation research 571, 121. 2005).

The inhibition of tyrosinase, which is an important enzyme in the biosynthesis of melanin, can reduce the production of melanin, which can be said to have a skin whitening effect, and this fact is known in the literature (J. Soc. Cosmet Chem., 42, 361, 1991)

Melanin biosynthesis inhibitors are currently being applied to medicines, cosmetics, foods and the like for treating skin diseases, skin whitening agents, food browning agents, and prevention of pigment deposition in the skin is mainly studied from four perspectives.

The first is to develop a tyrosinase synthesis inhibitor or a tyrosinase antagonist to control tyrosinase activity, the main enzyme of melanin synthesis.

The second is to develop substances that exhibit toxicity to melanocytes in order to lower the function of melanocytes, the site of melanin biosynthesis in animals.

The third is to develop dopa reducing materials to prevent oxidation of waveguides.

Finally, there is a method for reducing the activity of the enzymes that catalyze the conversion of the first enzyme tyrosinase, a melanin producing mechanism, and DHICA, from Dopachrome.

Representative examples of melanin production-inhibiting effective substances developed so far include arbutin, koji acid, ascorbic acid di-phosphate ester magnesium, vitamin C, hydroquinone, and plant extracts.

However, kojic acid acts to inhibit enzyme activity by chelating copper ions present in the active site of tyrosinase, and its inhibitory effect is excellent, but it is not suitable as a cosmetic because of its low safety when formulated in cosmetics.

Ascorbic acid phosphate ester magnesium is involved in the reaction to form melanin from tyrosine, thereby reducing the intermediate dopaquinone and inhibiting melanin production.

Another is that it acts directly on melanin to reduce dark oxidized melanin to brown reduced melanin, but it has a problem in stability due to rapid decomposition and coloring in solution.

Vitamin C and its derivatives also show whitening effect, but it is difficult to use it as a raw material for cosmetics because of instability which is well oxidized. Recently, research on vitamin stability has been actively conducted. Hightroquinone has irritating whitening action and is highly irritating to the skin, so its use is restricted due to safety problems. In recent years, it has been recognized as carcinogenic and its use as a cosmetic raw material is prohibited.

Most of the plant extracts exhibit a relatively high inhibitory effect on tyrosinase activity only at a high concentration, and the inhibitory effect is hardly exhibited at a low concentration.

DISCLOSURE OF THE INVENTION The present invention was conceived in order to solve the above-mentioned problems. First, the present invention relates to a cosmetic composition for preventing dark circles, dermatitis, allergy, atopy, eczema, psoriasis prevention and treatment, And to provide a cosmetic composition for skin whitening for whitening effect and wrinkle improvement by treatment.

It is another object of the present invention to provide a cosmetic composition for enhancing the immunity of the skin and preventing aging, regeneration of the cells and maintenance of skin elasticity, maintenance of moist and moist skin for a long time, quick skin absorption and skin whitening, .

In the meantime, the objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.

In order to achieve the above objects, the present invention provides a cosmetic composition comprising 69.150 wt% of water, 15.000 wt% of glycerin, 0.300 wt% of a thickener, 0.200 wt% of a skin elasticity agent, 0.500 wt% of hyaluronic acid, 1.000 wt% of a skin moisturizing and protecting agent, wt.%, skin conditioning agent 0.100 wt.%, mushroom extract 2.000 wt.%, shiitake mushroom extract 2.000 wt.%, rice flour 2.000 wt.%, ASTRIN- AG 2.000 wt.%, cynomolium extract 2.000 wt.%, skin solubilizing agent 0.200 wt. 3.000 wt%, FRANKINCENSE 0.020 wt%, PATCHOULI extract 0.020 wt%, essential oil 0.008 wt%, and orange extract 0.002 wt%.

The present invention has the following excellent effects.

First, it has excellent whitening effect and wrinkle-reducing effect by prevention of dark circles, prevention of dermatitis, allergy, atopy, eczema, psoriasis prevention and treatment, prevention of spots and freckles, and prevention of melanin synthesis due to ultraviolet rays.

In addition, it has an excellent effect of strengthening the immunity of the skin and preventing aging, regeneration of the cells and maintenance of skin elasticity, moisturizing and moisturizing the skin with time, quick skin absorption, and good fragrance for a long time.

FIGS. 1A to 1F are graphs showing the results of measuring tyrosinase inhibitory activity of a cosmetic composition for skin whitening according to an embodiment of the present invention. FIG.
FIGS. 2A to 2H are graphs showing the results of a cytotoxicity measurement experiment of a cosmetic composition for skin whitening according to an embodiment of the present invention. FIG.
FIGS. 3A to 3F are graphs showing results of experiments for measuring melanin production amount of a cosmetic composition for skin whitening according to an embodiment of the present invention. FIG.

The term used in the present invention is a general term that is widely used at present. However, in some cases, there is a term selected arbitrarily by the applicant. In this case, the term used in the present invention It is necessary to understand the meaning.

Hereinafter, the technical structure of the present invention will be described in detail with reference to preferred embodiments shown in the accompanying drawings.

First, a cosmetic composition for skin whitening according to an embodiment of the present invention comprises 69.150 wt% of water, 15.000 wt% of glycerin, 0.300 wt% of thickener, 0.200 wt% of skin elasticity agent, 0.500 wt% of hyaluronic acid, 1.000wt%, vitamin C 0.500wt%, skin conditioning agent 0.100wt%, mushroom extract 2.000wt%, shiitake mushroom extract 2.000wt%, rice flour 2.000wt%, ASTRIN-AG 2.000wt% 0.020 wt.% Of ethanol, 3.00 wt.% Of FRANKINCENSE, 0.020 wt.% Of PATCHOULI extract, 0.008 wt.% Of essential oil and 0.002 wt.% Of orange extract.

At this time, the water may use various distilled water, but DIWATER (Deionized water) is used in the preferred embodiment of the present invention.

Meanwhile, glycerin added to a cosmetic composition for skin whitening according to an embodiment of the present invention is added to protect skin and mucosal surfaces, and glycerin prepared by various methods can be used. There is no limitation.

Meanwhile, in the preferred embodiment of the present invention, the incrementer may utilize a variety of increment agents, and the increment agent of the natural ingredients extracted from sugarcane may be relatively easy to gel, Use KELTROL F.

In addition, the skin elasticity agent according to a preferred embodiment of the present invention preferably uses ALLANTOIN, and a synthetic method thereof is a method of obtaining cancellation of hydantoin and urea, A method of condensing one molecule of oxylic acid, a method of oxidizing uric acid with potassium permanganate under alkaline conditions, a method of condensing a derivative of urea and acetic ester with hydrogen chloride as a condensing agent, and the like.

Meanwhile, hyaluronic acid (HYALURONIC ACID) added to a cosmetic composition for skin whitening according to an embodiment of the present invention plays a role of preventing intrusion of bacteria or penetration of poison.

Meanwhile, a skin moisturizing and protecting agent is further added to the cosmetic composition for whitening skin according to an embodiment of the present invention. In this case, various moisturizing and protecting agents may be used as the skin moisturizing and protecting agent, In the preferred embodiment of the present invention, TORNARE is used.

According to an embodiment of the present invention, the skin conditioner may be made of a variety of ingredients as a component that changes the characteristics of the skin. In the present invention, the skin conditioner may be a diphosphoglyceric acid ).

Meanwhile, the skin solubilizer can dissolve the oil component added to the composition according to one embodiment of the present invention to make the product transparent, and various skin solubilizers can be used. In the examples, HCO-60 is used.

In addition, the essential oil added to the cosmetic composition for skin whitening according to an embodiment of the present invention is a highly volatile aromatic oil extracted from plants, and it is possible to use oils extracted from various plants. However, In the examples, CEDARWOOD HIMALAYAN (cedrus deodara) is used.

Hereinafter, experimental results of a cosmetic composition for skin whitening according to an embodiment of the present invention will be described in detail.

1A to 1F are graphs showing the results of measurement of tyrosinase inhibitory activity of a cosmetic composition for skin whitening according to an embodiment of the present invention, FIGS. 3A to 3F are graphs showing the results of measuring cytotoxicity of cosmetic compositions for whitening, and FIGS. 3A to 3F are graphs showing the results of measuring melanin production of cosmetic compositions for skin whitening according to an embodiment of the present invention.

1. Results of tyrosinase inhibitory activity

The tyrosinase inhibitory activity was measured by absorbance method using L-DOPA as a substrate. The mushroom tyrosinase was dissolved in 68 mM sodium phosphate buffer (pH 6.8) and stored at -20 ° C for 125 U / ml. Respectively. 40 μl of sample extract was added to 96 well plate, 120 μl of 8 mM L-DOPA was added, 40 μl of mushroom tyrosinase was added, and incubated for 20 minutes. Absorbance was measured with an ELISA reader at 490 nm.

Kojic acid is used as a control for comparing the efficacy of whitening.

MTT assay was performed to determine the range of concentrations to be used for the cytotoxicity measurement and experiment of each sample.

The cells were cultured in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (P / S).

Cells were suspended in 0.25% trypsin-EDTA when cells were 80 ~ 90% confluent, and then seeded at a density of 1 × 10 4 cells / well in a 96-well plate. After the cells were incubated overnight, the samples were diluted to the final concentration, added to each well in the same volume, and incubated for 24 hours at 37 ° C in a 5% CO 2 incubator. Then, MTT was added to each well, incubated for 4 hours, added with dimethyl sulfoxide (DMSO), and absorbance was measured at 540 nm using an ELISA reader to determine cell proliferation.

The samples used in the experiment are shown in Table 1 below.

number name Extraction condition One HTN-W1: rice husk 2 HTN-W2: Rice hull mushroom fungus 3 HTN-W3: rice husk mushroom fungus 4 HTN-W4: Hwanggi shiitake fungus 5 HTN-M1: rice bran 6 HTN-M2: rice bran mushroom fungus 7 HTN-M3: Mushroom mushroom 8 HTN-M4: rice bran mushroom Alcohol 80% 9 HTN-H1: brown rice 10 HTN-H2: brown rice mushroom 11 HTN-H3: brown rice mushroom fungus 12 HTN-H4: brown rice mushroom fungus 13 Situation mushroom 14 Mushroom 15 Shiitake mushrooms

As shown in FIGS. 1A to 1F, tyrosinase activity was slightly decreased at a low concentration of 15 samples, while kojic acid, which was used as a positive control, showed a remarkably inhibitory effect on tyrosinase activity in a concentration-dependent manner.

It is presumed that the sample inhibited the tyrosinase activity by affecting the metabolic process of the cell rather than acting directly on the tyrosinase.

2. Cytotoxicity test results

In this regard, the MTT assay was performed for the safety test of the material. As a result, the survival rate was 70% or more to 100 μg / ml as shown in FIGS. 2a to 2h.

3. Melanin production test results

In order to measure changes in the amounts of melanin synthesis in the cells, 15 kinds of extracts were treated at a concentration of 3, 10, 30, and 100 μg / ml and cultured for 3 days. The melanin production was measured by dissolving the cells, , The absorbance of the melanin was doubled in the case of α-MSH treatment alone, which promotes melanin production, as compared to the control without any reagent.

3A, when the α-MSH and HTN-H1,2,3 were treated together, the amount of melanin was significantly decreased, and that of HTN-H3 was significantly lower than that of HTN-H1. Referring to FIG. , HTN-W2,4 was significantly decreased compared to HTN-W1 when α-MSH and HTN-W1,2,3,4 were treated together, and the melanin content was significantly decreased. Melanin levels decreased significantly when MSH and HTN-M1, 2,3,4 were treated together.

The cosmetic composition for skin whitening according to an embodiment of the present invention is characterized by the above-described technical constitution and the result of the experiment, it is possible to prevent dark circles, dermatitis, allergy, atopy, eczema , Prevention and treatment of psoriasis, prevention of spots and freckles, and treatment of whitening effect and wrinkle improvement effect is excellent.

In addition, it has an excellent effect of strengthening the immunity of the skin and preventing aging, regeneration of the cells and maintenance of skin elasticity, moisturizing and moisturizing the skin with time, quick skin absorption, and good fragrance for a long time.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation in the present invention. Various changes and modifications may be made by those skilled in the art.

Claims (1)

In a whitening cosmetic composition,
A skin moisturizing and protecting agent 1.000wt%, a vitamin C 0.500wt%, a skin conditioning agent 0.100wt%, a skin conditioning agent 0.100wt%, a skin conditioning agent 0.200wt%, a hyaluronic acid 0.500wt% 2.000 wt% of ASTIN-AG, 2.000 wt% of ASTIN-AG, 2.00 wt% of skin extract, 0.200 wt% of skin solubilizing agent, 3.000 wt% of ethanol, 0.020 wt% of FRANKINCENSE, 0.020 wt% of PATCHOULI extract, 0.008 wt% of essential oil and 0.002 wt% of orange extract.

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