KR20170002084A - Cartrige for analysis and auxiliary device - Google Patents

Abstract

The cartridge for analysis and the auxiliary device applied thereto are provided. The analyzing cartridge includes an auxiliary member including a metering chamber capable of accommodating a predetermined amount of sample and a remaining chamber capable of accommodating a sample of the remaining amount exceeding the metering chamber.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a cartridges for analytical use,

The present disclosure relates to an analysis cartridge for analyzing a sample and an auxiliary device which can be applied to the above-described analysis cartridge.

Methods for measuring biometric information through samples taken from the body are still being developed. As urine or blood is generally used as a sample, and a measuring method is developed, a method of measuring biometric information such as diabetes using sweat or tears as a sample has been developed. In addition, a method for measuring biological information by collecting samples from saliva and exhaled breath is under development.

As a general method of measuring bio-information using a sample, biometrics information can be easily measured by qualitative analysis that determines the result of a reaction to a reagent on a strip with naked eyes and determines whether the sample is positive or negative.

In the measurement of biometric information, many reagent responses to various diseases are required, and quantitative judgment is required to judge the degree of the state through measurement values as well as qualitative analysis of positive / negative responses .

One embodiment relates to an assay cartridge in which a certain amount of sample can be injected for quantitative analysis and an auxiliary device that can be applied to the cartridge.

The following type of analytical cartridge according to one type includes an auxiliary member including a metering chamber capable of accommodating a certain amount of sample and a waste chamber capable of accommodating a sample of the remaining amount exceeding the metering chamber; A body through which a predetermined amount of the sample can be introduced into the metering chamber; And a valve for controlling the flow of the predetermined amount of sample to the main body.

The valve may include a blocking portion that can block the metering chamber and the main body.

In addition, the blocking portion may move in a direction transverse to the longitudinal direction of the auxiliary member.

The valve may further include a protrusion connected to the blocking portion and projecting to the outside of the analysis cartridge.

Further, the valve may be disposed between the auxiliary member and the main body.

And, the capacity of the metering chamber may be less than the capacity of the remaining chamber.

Further, the metering chamber and the remaining chamber may not overlap in the longitudinal direction of the auxiliary member.

The auxiliary member may include a first side wall defining an outer wall of the auxiliary member; And

And a second side wall dividing the metering chamber and the remaining chamber.

The length of at least a portion of the second sidewalls may be less than or equal to the length of the first sidewall.

The metering chamber may be surrounded by the remaining chamber.

In addition, the metering chamber may include a first metering chamber of a first capacity and a second metering chamber of a second capacity.

And, the first metering chamber and the second metering chamber may not overlap in the longitudinal direction of the auxiliary member.

In addition, the sample entering the first metering chamber and the second metering chamber may be the same.

The first metering chamber and the second metering chamber may overlap at least a part of the area in the longitudinal direction of the auxiliary member.

In addition, the sample flowing into the first metering chamber and the sample flowing into the second metering chamber may be different from each other.

The first capacity and the second capacity may be different from each other.

The apparatus may further include an inlet connected to the auxiliary member and through which the sample flows from the outside.

And, the inflow portion may include a tapered shape.

The main body may include a reaction member that reacts with the predetermined amount of the sample.

According to another aspect of the present invention, there is provided an auxiliary device for a cartridge, comprising: a metering chamber capable of receiving a predetermined amount of a sample; And a remaining chamber capable of receiving a sample of the remaining amount overflowing from the metering chamber.

1 is an exploded perspective view of an assay cartridge according to an embodiment.
Fig. 2 is a detailed view of the auxiliary member of Fig. 1. Fig.
3 is an exploded perspective view of the main body of Fig.
FIG. 4 is a view showing a strip that may be embedded in the body of FIG. 1. FIG.
5A to 5D are reference drawings illustrating a process of filling a metering chamber with a sample according to an embodiment.
6 is a view showing an assay cartridge according to another embodiment.
7 is a view showing an assay cartridge according to another embodiment.
8 is a view showing a structure in which a valve according to an embodiment passes through an auxiliary member.
9 is a diagram illustrating a plurality of metering chambers arranged in parallel according to another embodiment.
10 is a view illustrating a structure in which a plurality of metering chambers are arranged in series according to another embodiment.
11 is a view showing an assay cartridge including a main body in which a reaction member according to another embodiment is integrated.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings. Referring to the accompanying drawings, the same or corresponding components are denoted by the same reference numerals, .

The analytical sample according to one embodiment may be a liquid or a substance having fluidity similar to the liquid, with or without the target substance to be analyzed. The sample introduced into the strip for analysis may be accompanied by a pretreatment for modifying or altering the characteristics of the sample, or may be a material obtained directly from the source.

The source of the sample may be selected from the group consisting of blood, interstitial fluid, saliva, ocularlens fluid, cerebral spinal fluid, sweat, urine, ascites fluid, May be a biological source, such as a physiological fluid including raucous, synovial fluid, peritoneal fluid, vaginal fluid, amniotic fluid, and the like . However, it is not limited thereto. The source of the sample may be an environmental sample for water quality management or soil management, and does not limit the type of sample.

The target substance contained in the sample is a compound to be analyzed in the sample, which is also referred to as a target. For example, the target material may be selected from the group consisting of nucleic acid, high sensitivity C-reactive protein (hsCRP), microCRP, HbA1c (glycated hemoglobin), microalbumin, prostate specific antigen (PSA), alpha- fetoprotein (AFP), cTnI There may be prostate specific antigen (PSA), glucose, c-reactive protein (CRP), cortisol, DHEA-S (dehydroisoandrosterone sulphate) and the like.

FIG. 1 is an exploded perspective view of an assay cartridge 100 according to an embodiment, FIG. 2 is a specific view of the assistance member 110 of FIG. 1, and FIG. 3 is an exploded perspective view of the main body 120 of FIG. And FIG. 4 is a view showing a strip that may be embedded in the main body 120 of FIG.

As shown in FIGS. 1 and 2, the analysis cartridge 100 includes an auxiliary member 110 for separating a predetermined amount of a sample and a residual sample from the outside, a predetermined amount of sample from the auxiliary member 110, And a valve 130 for controlling the flow of the sample from the main body 120 and the auxiliary member 110 to the main body 120 by a predetermined amount.

The auxiliary member 110 can separate the sample introduced into the main body 120 into a certain amount of sample and a remaining amount of sample to provide a predetermined amount of the sample to the main body 120. The auxiliary member 110 may be integrated with the main body 120 or may be separated from the main body 120. When the auxiliary member 110 is separated from the main body 120, it is also referred to as an auxiliary device. 1, the auxiliary member 110 is illustrated as being separate from the main body 120. However, it is not limited thereto. The auxiliary member 110 may be integrated with the main body 120.

As shown in FIG. 2, the auxiliary member 110 may be formed as one frame. For example, the auxiliary member 110 includes a first side wall S1 forming the outside of the auxiliary member 110, a second side wall S2 dividing the internal space of the auxiliary member 110 into a plurality of spaces, And a lower wall S3 disposed below the member 110 and connecting the first sidewall S1 and the second sidewall S2.

The first sidewall S1 may be a closed curved surface such that a first hollow is formed therein. One end of the first hollow may be opened and the other end may be closed by the lower wall S3. The first hollow by the first sidewall S1 and the lower wall S3 may be a waste chamber 220 of the auxiliary member 110. [

The first hollow is shown in the form of a column in the longitudinal direction L of the auxiliary member 110. However, it is not limited thereto. The first hollow may include a tapered shape. For example, the first hollow may have a shape in which the size of the cross section decreases gradually from one end to the other end, or may have a shape in which the cross section gradually increases in size from one end to the other end. Or the first hollow may have the same cross-sectional size in some regions and the smaller cross-sectional size in the other regions.

The first hollow section is shown in a circular shape. However, it is not limited thereto. The cross section of the first hollow may be polygonal (e.g., triangular, square, etc.), circular or elliptical.

The second sidewall S2 may be a closed curved surface such that a second hollow is formed therein. One end of the second hollow may be opened and the other end may be closed or opened by a valve 130 described later. At least a portion of the second hollow by the second sidewall S2 and the valve 130 may be a metering chamber 210.

The second hollow is shown in a columnar shape in the longitudinal direction (L) of the auxiliary member (110). However, it is not limited thereto. The second hollow may include a tapered shape. Or the second hollow may have the same cross-sectional size in some regions and the smaller cross-sectional size in the other regions. The cross section of the second hollow is shown as a circle. However, it is not limited thereto. The cross section of the second hollow may be polygonal (e.g., triangular, square, etc.), circular or elliptical.

The height of the second sidewall S2 may be less than or equal to the height of the first sidewall S1. Although the height of the second sidewall S2 is smaller than the height of the first sidewall S1, the present invention is not limited thereto. The height of the second sidewall S2 may be equal to the height of the first sidewall S1 or may be less than the height of the first sidewall S1.

Thus, the assistance member 110 may include a metering chamber 210 capable of receiving a certain amount of sample and a remaining chamber 220 capable of accommodating a remaining amount of sample overflowing from the metering chamber 210. The metering chamber 210 and the remaining chamber 220 may be separated by a second sidewall S2. Although the figures show the metering chamber 210 as being surrounded by the remaining chamber 220, it is not limited thereto. The remaining chamber 220 and the metering chamber 210 may be sequentially arranged.

The assay cartridge 100 may further include a valve 130 disposed between the metering chamber 210 and the body 120 and for controlling whether the sample flows into the body 120 from the metering chamber 210 . The valve 130 may include a blocking portion 131 for blocking the second hollow. The capacity of the metering chamber 210 can be determined by the blocking portion 131 and the second side wall S2. The cross-sectional size of the blocking portion 131 may be greater than the cross-sectional size of the second hollow.

The valve 130 may further include a protrusion 132 connected to the blocking portion 131 and projecting to the outside of the assay cartridge 100. The user can open the second hollow by opening the blocking portion 131 by pulling the projection 132 in a direction transverse to the longitudinal direction L of the auxiliary member 110 while grasping the projection 132. [ For example, the user can pull the protrusion 132 in a direction perpendicular to the longitudinal direction L of the auxiliary member 110 while holding the protrusion 132. Then, the blocking portion 131 can also open the second hollow while moving in the direction perpendicular to the longitudinal direction L of the auxiliary member 110. Thus, the sample may flow from the metering chamber 210 to the body 120.

The valve not only removes the flow of the sample contained in the metering chamber 210 to the main body, but also serves to prevent the opening of the main body 120 from being exposed to air and foreign matter.

Although the valve 130 is shown in the figure as being disposed on the lower wall S3 of the auxiliary member 110, it is not limited thereto. The valve 130 may be arranged passing through a part of the first sidewall S1 and the second sidewall S2 of the frame.

The user may manually open the metering chamber 210 by pulling on the valve 130, but is not limited thereto. The valve 130 may also operate automatically.

3, the main body 120 includes a reaction member 300 that reacts with a sample, a first housing 410 that receives the reaction member 300 therein, and a second housing 410 that covers the upper end of the first housing 410 And may include a second housing 420.

The first housing 410 may include a strip receiving portion 411 for receiving the reaction member 300 and a first engaging portion 412 for engaging with the second housing 420. The strip receiving portion 411 may include a plurality of guides 413 protruding from the first housing 410. The guides 413 can prevent the reaction member 300 from being shaken as well as allowing the reaction member 300 to be positioned at a predetermined position. The first engaging portion 412 is engaged with the second engaging portion (not shown) disposed on the second housing 420 so that the second housing 420 is engaged with the first housing 410 to be closed.

The second housing 420 may include an opening 421 through which a sample can be introduced from the metering chamber 210 and a measurement window 422 through which the reaction result generated in the reaction member 300 can be measured or confirmed .

The opening 422 may be located at a location corresponding to the metering chamber 210 when the second housing 420 engages the first housing 410. The cross-sectional size of the opening 422 may be greater than or equal to the cross-sectional size of the metering chamber 210. The cross-sectional shape of the opening 422 may also depend on the cross-sectional shape of the metering chamber 210. The opening 422 may be formed in a circular shape, but is not limited thereto and may be formed in a polygonal shape.

The measurement window 422 is a window in which the reaction result of the reaction member 300 is exposed. Thus, the user can confirm the reaction result of the reaction member 300 through the measurement window 422. Although the measurement window 422 is shown as an ellipse, it is not limited thereto, and it may be formed in a polygonal shape or the like.

The shape of the first housing 410 and the second housing 420 may correspond to the shape of the reaction member 300. For example, when the reaction member 300 is polygonal, the first and second housings 210 and 220 may be polygonal, and when the reaction member 300 is circular, the first and second housings 210 and 210 , 220 may also have a circular shape. However, it is not limited thereto. The shapes of the first and second housings 210 and 220 may be irrelevant to the shape of the reaction member 300.

The auxiliary housing 110 of the cartridge for analysis, the first housing 410 and the second housing 420 of the main body 120 and the valve 130 may be made of a chemically stable synthetic resin and a combination thereof. For example, the auxiliary member 110 and the valve 130 may be made of a material selected from the group consisting of polyethylene, polypropylene, polystyrene, polyethylene terephthalide, polyamide, polyester, polyvinyl chloride, polyurethane, polycarbonate, polyvinylidene chloride, May be prepared using known molding methods using various thermosetting and thermoplastic plastics such as methylene, polyetherimide, and combinations thereof. However, the present invention is not limited thereto, and any material suitable for the purpose of the cartridge 100 can be used.

The reaction member 300 may be in the form of a strip capable of analyzing a sample using immunochromatography. 4, the reaction member 300 is arranged on the base member 310 and the base member 310 in a first direction, for example, in the longitudinal direction L A loading pad 320, a bonding pad 330, a membrane 340, and an absorbent pad 350. Here, the strip may mean a piece of material cut from a sheet to a required width. When the sample flows into the loading pad 320 through the opening 421, flow of the sample starts due to the capillary phenomenon. Thus, the sample may move along the bonding pad 330, the membrane 340, and absorbed by the absorbent pad 350, thus ending the movement.

In particular, the bonding pad 330 may be impregnated with a bonding agent capable of bonding with the target material in the sample. The conjugate may comprise a detector (e.g., a detection antibody) and chromogenic particles that are specifically attached to the target material. In the conjugate, the detectors and the coloring particles may be conjugated to each other. The sample introduced from the loading pad 320 is diffused into the bonding pad 330, and the first composite material can be formed by bonding the target material in the sample with the detector in the bonding agent.

The membrane 340 may include a test region 360 for detecting the target material and a control region 370 for detecting the conjugate. For example, in the examination region 360, a plurality of first capturing molecules (for example, a primary antibody) capable of specifically binding to a target substance in a sample may be arranged in a fixed state, The region 370 may be provided with a plurality of second captures that are specifically coupled to detectors of the conjugate. The inspection region 360 and the verification region 370 may be formed in a line shape across the longitudinal direction L of the membrane 340. The inspection region 360 and the verification region 370 can pass through the width direction of the membrane 340.

A plurality of first capturing members immobilized in a spot shape may be arranged in the inspection region 360 over a predetermined section of the membrane 340. The plurality of first capturing elements may be arranged in one-dimensional or two-dimensional arrangement.

The sample introduced from the bonding pad 330 may be selectively combined with the first capturing element of the inspection region 360 while being moved in the longitudinal direction L along the membrane 340 by capillary flow. For example, the target material in the sample may be bound to the conjugate through an antigen-antibody reaction at the bond pad 330 to become the first complex, and the first complex formed at the bond pad 330 may be the first Can be combined with the captor and become a second complex by the sandwich assay principle. Then, the second composite can be fixed in the inspection area 360. The first capturing member 120 is colored by the first coloring particles of the first complex. The number of the first captured captures may be proportional to the concentration of the target substance in the sample. Thus, it is possible to analyze the sample by measuring the density of the colored first captor.

Particularly, in order to quantitatively analyze the sample, information on the amount of the sample flowing into the reaction member 300 is required. Information on the sample can be obtained by measuring the amount of the sample flowing into the reaction member 300. Or the amount of the sample flowing into the reaction member 300 can be made constant. Since the assay cartridge 100 according to one embodiment includes the auxiliary member 110 including the metering chamber 210, a certain amount of sample can be introduced into the main body 120.

The capacity of the metering chamber 210 may be determined by the amount of the sample to be quantitatively analyzed. The capacity of the residual chamber 220 can be determined by the amount of the sample to be applied to the auxiliary member 110. Generally, the capacity of the residual chamber 220 may be greater than the capacity of the metering chamber 210. However, it is not limited thereto. The capacity of the remaining chamber 220 may be less than or equal to the capacity of the metering chamber 210.

For example, when the amount of the sample to be quantitatively analyzed is about 100 mu m and the amount of the sample that can be applied to the auxiliary member 110 is about 300 mu m, the capacity of the metering chamber 210 is about 100 mu m, The capacity of the chamber 220 may be about 250 탆 or more. Or the amount of the sample to be quantitatively analyzed is about 100 mu m and the amount of the sample that can be applied to the auxiliary member 110 is about 150 mu m, the capacity of the metering chamber 210 is about 100 mu m and the remaining chamber 220 ) May be about 50 탆 to about 100 탆.

5A to 5D are reference views illustrating a process of filling the metering chamber 210 with a sample according to one embodiment.

The sample 500 may enter the metering chamber 210. When the capacity of the sample 500 is smaller than the capacity of the metering chamber 210, the sample 500 may be filled only in the metering chamber 210, as shown in FIG. 5A. The sample 500 can continue to flow into the metering chamber 210. When the capacity of the sample 500 is larger than the capacity of the metering chamber 210, the sample 500 can be swollen upwards of the metering chamber 210 by surface tension, as shown in FIG. 5B.

If the amount of the sample 500 continuously increases, the sample remaining after filling the metering chamber 210 can be introduced into the remaining chamber 220 as shown in FIG. 5C. The surface of the sample 500 filled in the metering chamber 210 can be flattened as the sample 500 flows into the remaining chamber 220. [ Therefore, even if the sample 500 continues to flow into the auxiliary member 110, the sample 520 exceeding the metering chamber 210 is not filled in the metering chamber 210 but flows into the remaining chamber 220, (110) can separate the sample (500) from the sample (510) and the remaining sample (520) by a predetermined amount.

On the other hand, the user can open the metering chamber 210 by pulling the valve 130. 5D, a predetermined amount of the sample 510 accommodated in the metering chamber 210 may be introduced into the reaction member 300 through the opening 421 of the main body 120. Only a predetermined amount of the sample 510 stored in the metering chamber 210 may be introduced into the main body 120. Therefore, it is possible to quantitatively analyze the sample with the result of the reaction of the analysis cartridge 100.

6 is a view showing an assay cartridge 100a according to another embodiment. As shown in FIG. 6, the assay cartridge 100a may include an inlet 140 connected to the auxiliary member 110, through which the sample flows from the outside. One end of the inflow portion 140 is exposed to the outside and the other end of the inflow portion 140 is in contact with the auxiliary member 110. The inlet 140 also has a hollow to allow the fluid to flow.

The inlet 140 may have a tapered shape having a different cross-sectional size from the outside to the auxiliary member 110. For example, the inflow portion 140 may have a larger sectional size from the outside to the auxiliary member 110. Thus, the user can collect samples such as saliva and spit them to the inflow unit 140 at a time. However, it is not limited thereto. The inlet 140 may have a smaller cross-sectional size from the outside to the auxiliary member 110. The inflow portion 140 may be formed integrally with the auxiliary member 110 or may be separated. The inflow conduit 140 may also be made of chemically stable synthetic resin and combinations thereof.

7 is a view showing an assay cartridge 100b including an auxiliary member 110 according to another embodiment. As shown in FIG. 7, the auxiliary member 110a shown in FIG. 7 is compared with the auxiliary member 110 shown in FIG. 1, and the second side wall S2 of the auxiliary member 110 shown in FIG. And the second sidewall S2 and the blocking portion 131 form a metering chamber 210. The metering chamber 210 is formed by a first sidewall S2 and a second sidewall S2. On the other hand, the auxiliary member 110a shown in FIG. 7 does not form the closed curved surface of the second side wall S2. One end of the second sidewall S2 is in contact with the first sidewall S1 and the other end of the second sidewall S2 is in contact with the first sidewall S1. Thus, the second sidewall S2, a portion of the first sidewall S1, and the blocking portion 131 form the metering chamber 210, and the second sidewall S2, the remainder of the first sidewall S1, (S3) can form the remaining chamber 220.

The length of at least a portion of the second sidewall S2 may be less than the length of the first sidewall S1. Thus, the channel 230 may be formed on the second sidewall S2. A sample exceeding the metering chamber 210 may flow to the remaining chamber 220 through the channel 230.

FIG. 8 is a view showing a structure in which the valve 130 according to one embodiment passes through the auxiliary member 110. FIG. As shown in FIG. 8, the valve 130 may block the second hollow through the openings 230 of the first sidewall S1 and the second sidewall S2. Since the valve 130 is disposed within the auxiliary member 110, a portion of the second hollow can be the metering chamber 210.

A plurality of metering chambers 210 may be arranged in the auxiliary member 110. 9 is a diagram illustrating a plurality of metering chambers arranged in parallel according to another embodiment.

As shown in FIG. 9, the assistance member 110 may include a first metering chamber 211 of a first capacity, a second metering chamber 212 of a second capacity, and a remaining chamber 220. The first metering chamber 211 corresponds to a second hollow defined by the second sidewall S2 and the second metering chamber 212 may correspond to a third hollow formed by the third sidewall S3. And, the remaining chamber 220 may correspond to a space excluding the second and third hollows in the first hollow formed by the first side wall S1. That is, the remaining chamber 220 may be disposed so as to surround the first and second metering chambers 211 and 212.

The capacity of the first metering chamber 211 and the second metering chamber 212 may vary depending on the type of target material to be analyzed. Thus, the capacities of the first metering chamber 211 and the second metering chamber 212 may be the same or different.

The first and second metering chambers 211 and 212 shown in FIG. 9 may be similar in shape to the metering chamber 210 shown in FIG. 1, but are not limited thereto. The first and second metering chambers 211 and 212 may be similar to the metering chamber 210 shown in Figure 6 and any of the first and second metering chambers 211 and 212 may be similar to the metering chambers shown in Figure 1 Similar to the metering chamber 210, and the remainder may be similar to the metering chamber 210 shown in FIG. The reaction members 300 may be connected to each of the plurality of metering chambers 210 one by one.

One kind of sample may be introduced into the plurality of metering chambers 210. A plurality of target materials may be included in one sample. For example, if blood is a sample, blood may include TnI (Troponin I) and Myoglobin to check for cardiovascular disease, glucose to check for diabetes, and CTx (C-terminal telopeptide) to diagnose osteoporosis.

Diabetes can be checked for glucose and CTx (C-terminal telopeptide) for diagnosis of osteoporosis can be included. Thus, the sample housed in the first metering chamber 211 can be used to detect glucose, and the sample housed in the second metering chamber 212 can be used to detect CTx. Of course, the plurality of metering chambers 210 may be used to analyze one target material. The assistant member 110 may include a plurality of metering chambers 210 to reduce the number of times of repeated analysis of the sample.

10 is a view illustrating a structure in which a plurality of metering chambers are arranged in series according to another embodiment.

10, the auxiliary member 110d includes a first auxiliary member 111 and a second auxiliary member 112 sequentially disposed in the longitudinal direction L of the auxiliary member 110 can do. The first auxiliary member 111 includes a first metering chamber 213 of a first capacity and a first remaining chamber 221 for accommodating a remaining amount of sample exceeding the first metering chamber 213, The member 112 may include a second metering chamber 214 of a second capacity and a second remaining chamber 222 to receive a remaining amount of sample beyond the second metering chamber 214. The second capacity may be smaller than the first capacity.

At least a portion of the region of the first metering chamber 213 may overlap the second metering chamber 210 in the longitudinal direction L of the assistance member 110d. A first valve 131 may be disposed between the first metering chamber 213 and the second metering chamber 214. Thus, the first valve 131 may block or open the sample flow between the first metering chamber 213 and the second metering chamber 214. Thus, a sample in the first metering chamber 213 can be introduced into the second metering chamber 214 when the first metering chamber 213 is opened. The sample may fill the second metering chamber 214 and the remaining sample may flow to the second remaining chamber 222.

The first metering chamber 213 and the second metering chamber 214 are arranged in series so that the sample can be pretreated before entering the reaction member 300. For example, a reagent for pretreatment of the sample may be placed in the first metering chamber 213. After the sample is introduced into the first metering chamber 213 and is pretreated with reagents, a predetermined amount of the pretreated reagent may be introduced into the second metering chamber 214. A predetermined amount of pre-treated reagent flows into the reaction member 300, thereby increasing the accuracy of quantitative analysis. Of course, even if the auxiliary member has one metering chamber, the reagent for the pretreatment may be placed in the metering chamber.

In Fig. 3, a reaction member which can be separated from the body is shown. However, it is not limited thereto. The reaction member can be integrated with the body. That is, the reaction chamber may be disposed in a part of the main body.

11 is a view showing an assay cartridge including a main body in which a reaction member according to another embodiment is integrated. Comparing FIG. 1 and FIG. 11, the main body 120a may include at least one flow path 622 through which the sample flows, and at least one reaction chamber 625 in which the reagent reacts with the sample. Specifically, the main body 120a may be formed as a structure in which three plates are joined as shown in Fig. The first plate 610a and the second plate 610b may be divided into a first plate 610a, a second plate 610b and a third plate 610c. have. Thus, it is possible to protect the sample in flow from the external light from the reaction chamber 625 or to prevent errors in the measurement of the optical characteristics in the reaction chamber 625.

The first plate 610a and the second plate 610b may be formed in a film form. However, the thicknesses of the first plate 610a, the second plate 610b, and the third plate 610c are merely examples, and the thickness of the main body 120a is not limited. The flow path 622 and the reaction chamber 625 may be formed in the third plate 610c by an opening.

A reaction chamber 625 is formed in a region of the third plate 610c opposite to the inlet 621c. In one embodiment, a region of the third plate 610c corresponding to the reaction chamber 625 The reaction chamber 625 can be formed by removing a predetermined shape such as a circle or a square. Since portions corresponding to the reaction chambers 625 of the first and second plates 610a and 610b are not pierced, when a predetermined region is removed from the third plate 610c, a reaction chamber 625 may be formed. For example, if a hole is formed in the third plate 610c, it can be a reaction chamber 625 soon. Alternatively, the fine storage container may be disposed in the removed area of the third plate 610c and used as the reaction chamber 625.

In addition, when a region of the first plate 610a and the second plate 610b corresponding to the reaction chamber 625 is transparent, the reaction occurring in the reaction chamber 625 or the resultant can be easily exposed to the outside.

Various reactions for fluid analysis can be performed in the reaction chamber 625. In an embodiment of the present invention in which the blood is used as a fluid, the reaction chamber 625 reacts with specific components of blood (particularly plasma) Or the reagent which discolors in advance can be accommodated in the reaction chamber and the color expressed in the other reaction chamber can be optically detected and quantified. Through the above-described numerical values, it is possible to confirm the presence of a specific component in the blood or the ratio of a specific component.

Many embodiments other than the above-described embodiments are within the scope of the claims of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

100, 100a, 100b, 100c: Cartridge for analysis
110:
120:
130: Valve
210: metering chamber
220: Residual chamber

Claims (20)

An auxiliary member including a metering chamber capable of accommodating a certain amount of sample and a remaining chamber capable of accommodating a sample of the remaining amount exceeding the metering chamber;
A body through which a predetermined amount of the sample can be introduced into the metering chamber; And
And a valve for controlling the flow of the predetermined amount of sample to the main body. The method according to claim 1,
Wherein the valve comprises:
And a blocking portion capable of blocking between the metering chamber and the main body. 3. The method of claim 2,
The cut-
And movable in a direction transverse to the longitudinal direction of the auxiliary member. 3. The method of claim 2,
Wherein the valve comprises:
And a protrusion connected to the blocking portion and projecting to the outside of the analysis cartridge. The method according to claim 1,
Wherein the valve comprises:
And an analyzing cartridge disposed between the auxiliary member and the main body. The method according to claim 1,
Wherein the capacity of the metering chamber is less than or equal to the capacity of the remaining chamber. The method according to claim 1,
Wherein the metering chamber and the remaining chamber comprise:
Wherein the auxiliary member is not overlapped in the longitudinal direction of the auxiliary member. The method according to claim 1,
The auxiliary member
A first sidewall forming an outer wall of the auxiliary member; And
And a second sidewall defining the metering chamber and the remaining chamber. 9. The method of claim 8,
Wherein the length of at least some of the second sidewalls is less than or equal to the length of the first sidewall. The method according to claim 1,
Wherein the metering chamber comprises:
And an analyzing cartridge surrounded by the residual chamber. The method according to claim 1,
Wherein the metering chamber comprises:
A first metering chamber of a first volume and a second metering chamber of a second volume. 12. The method of claim 11,
Wherein the first metering chamber and the second metering chamber,
Wherein the auxiliary member is not overlapped in the longitudinal direction of the auxiliary member. 13. The method of claim 12,
Wherein the sample entering the first metering chamber and the second metering chamber are identical. 12. The method of claim 11,
Wherein the first metering chamber and the second metering chamber,
And at least a part of the area overlaps the longitudinal direction of the auxiliary member. 15. The method of claim 14,
Wherein the sample flowing into the first metering chamber and the sample flowing into the second metering chamber are different from each other. 12. The method of claim 11,
Wherein the first capacity and the second capacity are different from each other. The method according to claim 1,
And an inlet connected to the auxiliary member and through which the sample flows from the outside. 18. The method of claim 17,
The inlet
An analytical cartridge comprising a tapered shape. The method according to claim 1,
The main body includes:
And a reaction member for reacting with the predetermined amount of the sample. A metering chamber capable of receiving a quantity of sample; And
And a remaining chamber capable of receiving a sample of the remaining amount overflowing from the metering chamber.

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