KR20160017880A - Cultivating method of mushroom using sawdust media - Google Patents

Cultivating method of mushroom using sawdust media Download PDF

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KR20160017880A
KR20160017880A KR1020140101424A KR20140101424A KR20160017880A KR 20160017880 A KR20160017880 A KR 20160017880A KR 1020140101424 A KR1020140101424 A KR 1020140101424A KR 20140101424 A KR20140101424 A KR 20140101424A KR 20160017880 A KR20160017880 A KR 20160017880A
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mushroom
weight
sawdust
medium
cultivation
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KR101645802B1 (en
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김수문
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김수문
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/23Wood, e.g. wood chips or sawdust
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/02Other organic fertilisers from peat, brown coal, and similar vegetable deposits
    • C05F11/04Horticultural earth from peat

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  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The present invention provides a method for cultivating a mushroom using a sawdust medium. The method for cultivating a mushroom using a sawdust medium comprises: a step of manufacturing a sawdust medium for mushroom cultivation comprising oak tree sawdust, calcium carbonate, wheat bran, cottonseed bran, brown sugar, water, nano-detoxification sulfur, and anthocyan; a step of inserting the sawdust medium into a cultivation container, sealing the cultivation container, and then heating and cooling the cultivation container for sterilization; a step of injecting mushroom spawn into the cooled sawdust medium; a step of cultivating the mushroom spawn in the dark to cultivate a mycelium; a step of cultivating the mushroom mycelium which has undergone dark cultivation in a lit environment to allow browning; and a step of inserting the cultivation container containing the browned mushroom mycelium into a cultivation facility to grow the mushroom mycelium into a mushroom.

Description

톱밥배지를 이용한 버섯의 재배방법{CULTIVATING METHOD OF MUSHROOM USING SAWDUST MEDIA}TECHNICAL FIELD The present invention relates to a method for cultivating mushroom using a sawdust medium,

본 발명은 톱밥배지를 이용한 버섯의 재배방법에 관한 것으로, 보다 상세하게는 배지에 접종되는 종균의 활력을 부여하고, 잡균 오염 방지 및 버섯의 생장을 촉진시킴과 동시에 수확량을 증가시킬 수 있어 버섯재배농가의 소득증진에 기여하고, 나아가 유황 및 안토시안이 강화된 기능성 버섯을 재배할 수 있어 국민건강 증진효과도 제공하는 톱밥배지를 이용한 버섯의 재배방법에 관한 것이다.
More particularly, the present invention relates to a method of cultivating mushroom using a sawdust medium, and more particularly, to a method of cultivating mushroom, And also to a method of cultivating mushroom using sawdust medium which is capable of cultivating functional mushroom enhanced in sulfur and anthocyanin and also providing a health promotion effect to the public.

일반적으로, 버섯류는 광합성을 하지 못하고 유기물 형태의 탄소를 이용하는 영양 요구성 미생물로서 분류학상 담자균류에 속한다. In general, mushrooms are nutrient-requiring microorganisms that do not have photosynthesis but utilize organic carbon.

담자균류에 속하는 버섯(mushroom)은 각종 유기물을 분해하여 성장 발육하는 자실체를 가지고 있으며, 일반 채소류에 비해 높은 영양가를 가지고 있는 식품재료이면서, 고혈압, 당뇨 및 항암효과도 탁월한 것으로 알려져 있는 대표적인 건강식품의 하나이다.Mushroom belonging to the basidiomycetes has fruiting body that grows and develops by decomposing various organic materials. It is a food material which has a higher nutritional value than ordinary vegetables, and is a typical health food which is known to excel in hypertension, diabetes and anticancer effect It is one.

최근에는 생활수준이 향상되어 국민보건에 관심이 고조되면서 청정농산물 재배 및 고영양 식품에 점점 관심이 집중되고 있어, 원하는 영양소나, 원소 또는 물질을 기존의 농축산물에 함유시켜 그 부가가치를 높이려는 노력이 계속되고 있다.
In recent years, as the level of living has improved and interest in the public health has increased, interest in concentrating clean agricultural products and high nutritional foods has been increasing, and efforts have been made to add the desired nutrients, elements or substances to existing agricultural products, Is continuing.

한편, 이러한 버섯류의 재배방법으로는 활엽수 등의 원목을 이용하여 재배하는 원목재배법과 일정한 설비를 갖춘 시설에서 배양하는 시설재배법이 알려져 있다. 그러나, 위 원목재배법은 관리하기가 간편하고 시설비가 적게 드는 장점이 있는 반면에, 원료량에 비해 버섯의 회수율이 낮고 원목 구입이 곤란하여, 최근에는 다양한 종류의 용기에 배지를 넣고 접종하여 일정한 시설에서 재배하는 시설재배법이 널이 이용되고 있다.On the other hand, as a method of cultivating these mushrooms, there is known a method of growing the logs using hardwoods such as hardwoods and a method of cultivating them in facilities having certain facilities. However, the above-mentioned timber cultivation method is easy to manage and has a small facility cost. On the other hand, the recovery rate of mushroom is low compared to the amount of raw material, and it is difficult to purchase timber. Recently, Is growing rapidly in Korea.

이때, 전술한 시설재배법에서는 통상 톱밥배지에 종균을 접종하여 제조된 배지를 이용하게 되고, 이 톱밥배지 자체를 상자에 담아 단상 재배를 하고 있으며, 이러한 단상 재배법은 이미 널리 공지된 기술이기 때문에 상세한 설명은 생략하기로 한다. 부가적으로 상기 재배법은 국내 공개특허공보 공개번호 제 85-909 호(활엽수 톱밥을 이용한 표고버섯 재배법) 및 공개번호 제 90-7303 호(표고의 톱밥 재배 방법)에 상세히 기술되어 있다.At this time, in the aforementioned facility cultivation method, the medium produced by inoculating the seeds into the sawdust medium is usually used, and the sawdust medium itself is packed in a box to carry out single-phase cultivation. Since such a single-phase cultivation is a well- Is omitted. In addition, the cultivation method is described in detail in Korean Patent Laid-Open Publication No. 85-909 (Method for cultivating shiitake using hardwood sawdust) and Publication No. 90-7303 (Method for cultivating sawdust in an elevation).

이러한 종래 단상 재배법에 의하면 종균의 활력이 떨어지고, 잡균 내지 곰팡이에 의한 오염문제와 더불어 수확량이 크지 않고, 더욱이 종래 버섯들과는 차별화를 기할 수 없는 획일적인 상품만을 수확할 수 있을 뿐이다. According to the conventional single-phase cultivation method, the vitality of seed bacterium is lowered, the problem of contamination by germs and fungi is not solved, and only a uniform product which can not differentiate from conventional mushrooms can be harvested.

농촌의 이촌현상과 노동인력의 노령화 경향이 심화되면서 버섯재배는 이와 같이 경제성에 한계를 보이고 있으며, 이에 과학적이고도 고부가가치의 버섯재배영농이 시급이 요청되고 있다.
As the trend of rural agriculture and the aging of the labor force are intensifying, mushroom cultivation is limited in economic efficiency, and scientific and high value added mushroom cultivation is urgently needed.

본 발명은 상기한 바와 같은 종래기술이 가지는 문제를 해결하기 위해 안출된 것으로, 그 목적은 배지에 접종되는 종균의 활력을 부여하고, 잡균 오염 방지 및 버섯의 생장을 촉진시킴과 동시에 수확량을 증가시킬 수 있어 버섯재배농가의 소득증진에 기여하고, 나아가 유황 및 안토시안이 강화된 기능성 버섯을 재배할 수 있어 국민건강 증진효과도 제공하는 톱밥배지를 이용한 버섯의 재배방법을 제공함에 있다.
DISCLOSURE Technical Problem The present invention has been conceived to solve the problems of the prior art as described above, and it is an object of the present invention to provide vigor of the seedling inoculated to the medium, to prevent contamination of the germs and to promote the growth of mushroom, The present invention provides a mushroom cultivation method using a sawdust medium which contributes to the promotion of income of a mushroom cultivator and further enables functional mushrooms enhanced in sulfur and anthocyanin to be cultivated, thereby providing a public health promotion effect.

상기한 바와 같은 본 발명의 기술적 과제는 다음과 같은 수단에 의해 달성되어진다.The technical problem of the present invention as described above is achieved by the following means.

(1) 참나무톱밥, 탄산칼슘, 소맥피, 면실피, 흑설탕, 물, 나노제독유황 및 안토시안을 포함하는 버섯재배용 톱밥배지를 제조하는 단계; 상기 톱밥배지를 배양용기에 담고 밀봉한 후, 살균을 위해 가열하고 냉각하는 단계;.상기 냉각된 톱밥배지에 버섯 종균을 접종하는 단계; 상기 버섯 종균을 암배양을 통해 균사체를 배양하는 단계; 상기 암배양을 거친 버섯 균사체를 명배양하여 갈변화를 진행시키는 단계; 및 상기 갈변화가 완료된 버섯균사체를 포함하는 배양용기를 재배사에 입사하여 버섯으로 배양하는 단계를 포함하는 톱밥배지를 이용한 버섯의 재배방법.
(1) preparing a sawdust medium for mushroom cultivation comprising oak sawdust, calcium carbonate, wheat bran, cotton bark, brown sugar, water, nano-desorbed sulfur and anthocyanin; The method comprising: inoculating the sawdust medium into a culture container, sealing the mixture, heating and cooling the paste medium for sterilization, inoculating the cooled sawdust medium with mushroom seeds, Culturing the mycelium by culturing the mushroom strain in an arm culture; Culturing the mushroom mycelium which has undergone the arm culturing and culturing the mushroom mycelium; And the mushroom mycelium which has undergone the change of browning is introduced into a grower and cultured in a mushroom.

(2) 상기 (1)에 있어서,(2) In the above (1)

참나무톱밥 40~50 중량%, 탄산칼슘 0.1~1.0 중량%, 소맥피 5~10 중량%, 면실피 1~5 중량%, 흑설탕 0.1~1.0 중량%, 물 30~50 중량%, 나노제독유황 0.01~0.1 중량% 및 안토시안 0.01~0.1 중량%를 포함하는 톱밥배지를 이용한 버섯의 재배방법.
The present invention relates to a method for producing a fermented soybean oil, comprising the steps of: preparing a soybean oil containing 40 to 50% by weight of oak sawdust, 0.1 to 1.0% by weight of calcium carbonate, 5 to 10% by weight of wheat bran, 1 to 5% To 0.1% by weight of anthocyanins and 0.01 to 0.1% by weight of anthocyanins.

(3) 상기 (1)에 있어서, (3) In the above (1)

피트모스 10~20 중량%를 더 포함하는 것을 특징으로 하는 톱밥배지를 이용한 버섯의 재배방법.
And 10 to 20% by weight of peat moss.

(4) 상기 (1)에 있어서, (4) In the above (1)

목초액 1~5 중량%를 더 포함하는 것을 특징으로 하는 톱밥배지를 이용한 버섯의 재배방법.
And 1 to 5% by weight of a woodpecker. The method of cultivating mushroom using the sawdust medium.

(5) 상기 (1)에 있어서, (5) In the above (1)

실크펩타이드 1~5 중량%를 더 포함하는 것을 특징으로 하는 톱밥배지를 이용한 버섯의 재배방법.
And 1 to 5% by weight of a silk peptide.

(6) 상기 (1)에 있어서, (6) In the above (1)

피트모스 10~20 중량%, 목초액 1~5 중량% 및 실크펩타이드 1~5 중량%를 더 포함하는 것을 특징으로 하는 톱밥배지를 이용한 버섯의 재배방법.
A peach moss 10 to 20% by weight, a vinegar solution 1 to 5% by weight, and a silk peptide 1 to 5% by weight.

(7) 상기 어느 하나의 방법에 의해 재배된 버섯.
(7) The mushroom grown by any one of the above methods.

상기와 같이 본 발명에 의하면, 배지에 접종되는 종균의 활력을 부여하고, 잡균 오염 방지 및 버섯의 생장을 촉진시킴과 동시에 수확량을 증가시킬 수 있어 버섯재배농가의 소득증진에 기여하고, 나아가 유황 및 안토시안이 강화된 기능성 버섯을 재배할 수 있어 국민건강 증진효과도 제공하는 효과를 제공한다.
As described above, according to the present invention, it is possible to increase the yield of the mushroom cultivator by imparting the vigor of the seeds inoculated to the medium, preventing the contamination of various kinds of bacteria and promoting the growth of the mushroom and contributing to the increase of the income of the mushroom cultivator, This enhanced functional mushroom can be cultivated, thus providing the effect of enhancing public health.

도 1은 본 발명에 따른 톱밥배지를 이용한 버섯의 재배공정도이다. FIG. 1 is a flow chart of mushroom cultivation using a sawdust medium according to the present invention.

본 발명에 따른 톱밥배지를 이용한 버섯의 재배방법은,The method for growing mushroom using the sawdust medium according to the present invention comprises:

참나무톱밥, 탄산칼슘, 소맥피, 면실피, 흑설탕, 물, 나노제독유황 및 안토시안을 포함하는 버섯재배용 톱밥배지를 제조하는 단계; 상기 톱밥배지를 배양용기에 담고 밀봉한 후, 살균을 위해 가열하고 냉각하는 단계;.상기 냉각된 톱밥배지에 버섯 종균을 접종하는 단계; 상기 버섯 종균을 암배양을 통해 균사체를 배양하는 단계; 상기 암배양을 거친 버섯 균사체를 명배양하여 갈변화를 진행시키는 단계; 및 상기 갈변화가 완료된 버섯균사체를 포함하는 배양용기를 재배사에 입사하여 버섯으로 배양하는 단계를 포함한다.
Preparing a sawdust medium for mushroom cultivation comprising oak sawdust, calcium carbonate, wheat bran, cotton bark, brown sugar, water, nano-decontamination sulfur and anthocyanin; The method comprising: inoculating the sawdust medium into a culture container, sealing the mixture, heating and cooling the paste medium for sterilization, inoculating the cooled sawdust medium with mushroom seeds, Culturing the mycelium by culturing the mushroom strain in an arm culture; Culturing the mushroom mycelium which has undergone the arm culturing and culturing the mushroom mycelium; And a mushroom mycelium which has undergone the change of browning, is introduced into a grower and cultured in mushrooms.

이하 본 발명의 내용을 도 1에 도시한 실시예를 참조하여 보다 상세하게 설명하면 다음과 같다.Hereinafter, the contents of the present invention will be described in more detail with reference to the embodiment shown in FIG.

본 발명에 따른 버섯재배용 톱밥배지는 바람직하게는 참나무톱밥 40~50 중량%, 탄산칼슘 0.1~1.0 중량%, 소맥피 5~10 중량%, 면실피 1~5 중량%, 흑설탕 0.1~1.0 중량%, 물 30~50 중량%, 나노제독유황 0.01~0.1 중량% 및 안토시안 0.01~0.1 중량%를 포함한다.The sawdust medium for mushroom cultivation according to the present invention preferably comprises 40 to 50% by weight of oak sawdust, 0.1 to 1.0% by weight of calcium carbonate, 5 to 10% by weight of wheat, 1 to 5% by weight of cottonseed, 0.1 to 1.0% 30 to 50% by weight of water, 0.01 to 0.1% by weight of nano-adsorbed sulfur and 0.01 to 0.1% by weight of anthocyanin.

상기 각 성분의 함량은 본 발명에서 바람직한 실시예로서 제시되는 범위로써, 상기 각 성분의 함량이 하한치를 미달하는 경우에는 버섯의 수확량이 감소하거나 원하는 생리활성을 제공하는 것이 어렵고, 상기 각 성분의 함량 범위의 상한치를 초과하게 되면 오히려 수확량이 떨어질 위험이 존재한다. When the content of each component is below the lower limit value, it is difficult to reduce the yield of the mushroom or to provide the desired physiological activity, and the content of each component If the upper limit of the range is exceeded, there is a risk that the yield will be lowered.

따라서, 본 발명의 바람직한 실시예로는 참나무톱밥 40~50 중량%, 탄산칼슘 0.1~1.0 중량%, 소맥피 5~10 중량%, 면실피 1~5 중량%, 흑설탕 0.1~1.0 중량%, 물 30~50 중량%를 조합하는 경우로써, 버섯의 활력증진과 수확량 증진 모두에 대하여 최적의 조건을 만족하게 된다.Accordingly, the preferred embodiment of the present invention is characterized by comprising 40 to 50 wt% of oak sawdust, 0.1 to 1.0 wt% of calcium carbonate, 5 to 10 wt% of wheat pest, 1 to 5 wt% of cottonseed, 0.1 to 1.0 wt% of brown sugar, 30 to 50% by weight of the mushroom is combined with the mushroom to satisfy the optimal conditions for both the enhancement of the vitality and the increase of the yield of the mushroom.

본 발명에서는 특히 나노제독유황을 톱밥배지에 첨가함으로써, 유황성분이 버섯의 내부로 이행할 수 있도록 함으로써, 유황성분이 제공하는 것으로 알려져 있는 항암작용, 각종 피부질환 개선, 정력회복, 콜레스테롤 합성억제 및 혈전분해, 뼈 강화, 염증제거 및 살균작용, 이뇨 및 변비억제작용 , 당뇨질환의 개선효과를 제공할 수 있다.In the present invention, especially by adding the nano-adsorbed sulfur to the sawdust medium, the sulfur component can be transferred to the inside of the mushroom, thereby improving the anticancer activity, various skin diseases, restoring tack, Thrombolysis, bone strengthening, inflammation removal and sterilization, diuretic and constipation-inhibiting action, and diabetic disease.

나노제독유황은 0.01~0.1 중량% 첨가하는 것이 바람직하며, 만일 0.01 중량% 미만으로 첨가할 경우 유황의 이행율이 현저히 떨어지고, 0.1 중량%를 초과할 경우에는 오히려 버섯의 생육을 저해하여 수확량을 떨어뜨릴 우려가 있어 상기 범위로 첨가한다.It is preferable to add 0.01 to 0.1% by weight of nano-adsorbed sulfur. If the amount is less than 0.01% by weight, the transition rate of sulfur is significantly lowered. If the content is more than 0.1% by weight, the growth of mushroom is inhibited, There is a possibility of kneading, and it is added in the above range.

또한 본 발명에서는 안토시안을 톱밥배지에 첨가하는데, 안토시안은 식물의 꽃이나 열매와 잎 등에 나타나는 수용성 색소를 말하며, 톱밥배지에 첨가하여 줌으로써 안토시안이 버섯내로 이행하도록 한다. 따라서, 안토시안이 내부에 포함된 버섯을 소비자가 섭취할 경우 상기 유황이 제공하는 생리활성효과에 더하여 추가적으로 강력한 항산화활성과 항암활성을 제공받게 된다.In the present invention, anthocyanin is added to the sawdust medium. Anthocyanin is a water-soluble pigment appearing on flower, fruit and leaf of a plant, and is added to sawdust medium to allow anthocyanin to migrate into the mushroom. Therefore, when the consumer consumes the mushroom contained in the anthocyanin, the antioxidant activity and the anticancer activity are additionally provided in addition to the physiological activity effect provided by the sulfur.

안토시안은 0.01~0.1 중량% 첨가하는 것이 바람직하며, 만일 0.01 중량% 미만으로 첨가할 경우 안토시안의 이행율이 현저히 떨어지고, 0.1 중량%를 초과할 경우에는 오히려 버섯의 생육을 저해하여 수확량을 떨어뜨릴 우려가 있어 상기 범위로 첨가한다.
Anthocyanin is preferably added in an amount of from 0.01 to 0.1% by weight, and if it is added in an amount of less than 0.01% by weight, the migration rate of anthocyanin is significantly lowered. If the content of the anthocyanin exceeds 0.1% by weight, And added in the above range.

또한 본 발명에 따른 톱밥배지는 피트모스, 목초액 및 실크펩타이드로 이루어진 군에서 선택되는 적어도 1이상의 성분을 더 포함하는 것이 바람직하다. 상기 피트모스, 목초액 및 실크펩타이드의 함량은 특별히 한정되는 것은 아니지만, 피트모스 10~20 중량%, 목초액 1~5 중량%, 실크펩타이드 1~5 중량%로 첨가하는 것이 바람직하다.The sawdust medium according to the present invention may further comprise at least one or more components selected from the group consisting of peat moss, vinegary and silk peptides. The content of the peat moss, vinegar solution and silk peptide is not particularly limited, but it is preferably 10 to 20 wt% peat moss, 1 to 5 wt% of wood vinegar, and 1 to 5 wt% of silk peptide.

상기 피트모스, 목초액 및 실크펩타이드를 톱밥배지에 첨가하는 것에 의해, 잡균이나 곰팡이류로부터 오염을 보다 완벽히 차단하는 것이 가능하고, 버섯의 생장에 필요한 각종 무기질, 및 영양성분을 충분히 공급받아 활력증진 효과뿐만 아니라 수확량을 보다 늘릴 수 있는 효과를 제공한다.
By adding the peat moss, vinegar solution and silk peptide to the sawdust medium, it is possible to more completely block contamination from germs and fungi, and it is possible to sufficiently supply various minerals and nutrients necessary for the growth of mushroom, It also has the effect of increasing the yield.

상기와 같은 본 발명에 따른 톱밥배지를 이용한 버섯의 재배방법은 도 1에 도시한 바와 같이, 먼저, 참나무톱밥, 탄산칼슘, 소맥피, 면실피, 흑설탕, 물, 나노제독유황 및 안토시안을 포함하는 배지조성물을 조제하는 과정이 필요하다.As shown in FIG. 1, the method for cultivating mushroom using the sawdust medium according to the present invention is as follows. First, as shown in FIG. 1, A process for preparing the medium composition is necessary.

이와 같이 조성한 톱밥배지를 배양용기에 담고 묶어 준 후, 배지용기를 살균기에 넣어 90~110℃, 바람직하게는 100℃에서 50~70분, 바람직하게는 60분, 120~125℃, 바람직하게는 121℃에서 80~100분, 바람직하게는 90분간 추가 살균한 후, 배지를 20~25℃로 냉각시켜 톱밥배지를 제조한다.
The sawdust medium thus prepared is placed in a culture container and bundled. The medium container is placed in a sterilizer at 90 to 110 ° C, preferably 100 ° C for 50 to 70 minutes, preferably 60 minutes, 120 to 125 ° C, After further sterilization at 121 DEG C for 80 to 100 minutes, preferably 90 minutes, the medium is cooled to 20 to 25 DEG C to prepare a sawdust medium.

상기와 같이 멸균된 톱밥배지를 이용하여 버섯을 재배하는 방법은 아래와 같다. 본 발명에 따른 톱밥배지는 다양한 버섯에 대하여 재배가 가능하지만, 바람직하게는 표고버섯을 들 수 있다.The method of growing the mushroom using the sterilized sawdust medium as described above is as follows. The sawdust medium according to the present invention can be cultivated on various mushrooms, but preferably includes mushroom.

상기와 같이 제조한 톱밥배지에 대하여 무균실에서 표고버섯의 종균을 접종한 후, 접종된 배지를 20~25℃, 바람직하게는 21~22℃, 습도 50% 이상, 바람직하게는 60~70%에서 30~60일 바람직하게는 30일 동안 암배양한다.The thus prepared sawdust medium is inoculated with a mildew of the shiitake in a clean room and then the inoculated medium is cultured at 20 to 25 ° C, preferably at 21 to 22 ° C, at a humidity of 50% or more, preferably 60 to 70% 30 to 60 days, preferably 30 days.

암배양이 종료된 후, 갈변을 발생시키고 배지 전체로 확장시키기 위해 30~60일, 바람직하게는 30일간 햇빛을 제공하여 명배양을 진행시킨다. 이와 같이, 갈변을 배지 전체적으로 진행시킨 후, 재배사에 입상하게 된다.After termination of the dark culture, light cultivation is carried out by providing sunshine for 30 to 60 days, preferably 30 days, in order to cause browning and extend to the entire medium. As described above, browning proceeds on the whole of the culture medium, and then the culture is started.

본 발명에 의하면, 입사이후 대략 5~8일, 바람직하게는 1주일 배양한 후 버섯을 수확할 수 있다.
According to the present invention, the mushroom can be harvested after culturing for about 5 to 8 days, preferably one week, after incidence.

이하 본 발명의 내용을 실시예에 의해 보다 상세하게 설명하기로 한다. 다만 이들 실시예는 본 발명의 내용을 이해하기 위해 제시되는 것일 뿐 본 발명의 권리범위가 이들 실시예에 한정되어지는 것으로 해석되어져서는 아니된다.
Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood, however, that these examples are provided so that the scope of the present invention is not limited thereto.

[실시예 1] [Example 1]

참나무톱밥 2280kg, 탄산칼슘 28kg, 소맥피 400kg, 면실피 100kg, 흑설탕 24kg, 물 1700L, 나노제독유황 1.7L 및 안토시안 1.7L를 배합하여 1400개의 버섯재배용 톱밥배지를 조성하였다.
1400 pieces of sawdust medium for mushroom cultivation were prepared by blending 2280 kg of oak sawdust, 28 kg of calcium carbonate, 400 kg of wheat bran, 100 kg of cottonseed, 24 kg of brown sugar, 1700 L of water, 1.7 L of nadoxid sulfur and 1.7 L of anthocyanin.

[실시예 2] [Example 2]

참나무톱밥 2280kg, 피트모스 100kg, 탄산칼슘 28kg, 소맥피 400kg, 면실피 100kg, 흑설탕 24kg, 물 1700L, 나노제독유황 1.7L 및 안토시안 1.7L를 배합하여 1400개의 버섯재배용 톱밥배지를 조성하였다.
1400 pieces of sawdust medium for mushroom cultivation were prepared by blending 2280 kg of oak sawdust, 100 kg of peat moss, 28 kg of calcium carbonate, 400 kg of wheat bran, 100 kg of cottonseed, 24 kg of brown sugar, 1700 L of water, 1.7 L of nano-demineralized sulfur and 1.7 L of anthocyanin.

[실시예 3] [Example 3]

참나무톱밥 2280kg, 탄산칼슘 28kg, 소맥피 400kg, 면실피 100kg, 흑설탕 24kg, 물 1700L, 목초액 25kg, 나노제독유황 1.7L 및 안토시안 1.7L를 배합하여 1400개의 버섯재배용 톱밥배지를 조성하였다.
1400 pieces of sawdust medium for mushroom cultivation were prepared by blending 2280 kg of oak sawdust, 28 kg of calcium carbonate, 400 kg of wheat bran, 400 kg of cottonseed flour, 24 kg of brown sugar, 1700 L of water, 25 kg of wood vinegar, 1.7 L of sulfurized nano and 1.7 L of anthocyanin.

[실시예 4] [Example 4]

참나무톱밥 2280kg, 탄산칼슘 28kg, 소맥피 400kg, 면실피 100kg, 흑설탕 24kg, 물 1700L, 실크펩타이드 25kg, 나노제독유황 1.7L 및 안토시안 1.7L를 배합하여 1400개의 버섯재배용 톱밥배지를 조성하였다.
1400 pieces of sawdust medium for mushroom cultivation were prepared by blending 2280 kg of oak sawdust, 28 kg of calcium carbonate, 400 kg of cottonseed, 400 kg of cottonseed, 24 kg of brown sugar, 1700 L of water, 25 kg of silk peptide, 1.7 L of nano-demolished sulfur and 1.7 L of anthocyanin.

[실시예 5] [Example 5]

참나무톱밥 2280kg, 피트모스 100kg, 탄산칼슘 28kg, 소맥피 400kg, 면실피 100kg, 흑설탕 24kg, 물 1700L, 목초액 25kg, 실크펩타이드 25kg, 나노제독유황 1.7L 및 안토시안 1.7L를 배합하여 1400개의 버섯재배용 톱밥배지를 조성하였다.
1400 pieces of sawdust for cultivation of mushrooms were prepared by blending 2280 kg of oak sawdust, 100 kg of peat moss, 28 kg of calcium carbonate, 400 kg of wheat bran, 100 kg of cottonseed, 24 kg of brown sugar, 1700 L of water, 25 kg of wood vinegar, 25 kg of silk peptide, Respectively.

[실험예 1] 톱밥배지를 이용한 표고버섯의 재배[Experimental Example 1] Cultivation of shiitake mushroom using sawdust medium

실시예 1 내지 5에서 조성한 톱밥배지를 지름 14㎝, 높이 12㎝의 원통의 비닐봉지에 담고 묶어 주었다. 배지용기를 살균기에 넣어 100℃에서 60분 121℃에서 90분간 살균한 후, 배지를 20~25℃로 냉각시켰다.The sawdust media prepared in Examples 1 to 5 were placed in a cylindrical plastic bag having a diameter of 14 cm and a height of 12 cm and bundled. The medium container was placed in a sterilizer and sterilized at 100 ° C for 60 minutes at 121 ° C for 90 minutes, and then the medium was cooled to 20 to 25 ° C.

상기와 같이 멸균된 배지에 무균실에서 표고버섯의 종균을 접종한 후, 접종된 배지를 22℃, 습도 60%에서 30일 동안 암배양하였다. 암배양이 종료된 후, 추가적으로 30일 간 명배양을 통해 갈변을 배지 전체적으로 진행시킨 후, 재배사에 입상하여 1주일 배양한 후 표고버섯을 수확하였다.After the inoculated medium was inoculated to the sterilized medium as described above, the inoculated medium was cultured for 30 days at 22 ° C and 60% humidity for 30 days. After the completion of the incubation period, the browning was progressed through the medium for 30 days, followed by incubation for 1 week in a cultivar, and shiitake was harvested.

비교예로 참나무톱밥과 미강을 주원료로 하는 시판 톱밥배지를 이용하여 동일한 조건하에 표고버섯을 재배한 결과 수확량에서 실시예 1은 용기 1개당 평균 10%, 실시예 2는 12%, 실시예 3 및 실시예 4는 14%, 및 실시예 5는 17%의 증가가 관찰되었다.
As a comparative example, when mushroom was cultivated under the same conditions using oak sawdust and commercial sawdust medium containing rice bran as the main raw material, the yield was 10% on average in Example 1, 12% in Example 2, An increase of 14% for Example 4 and 17% for Example 5 was observed.

[실험예 2] 유황 및 안토시안 함량[Experimental Example 2] Sulfur and anthocyanin content

실시예 1 내지 5에서 수확한 표고버섯을 대상으로 비교예에 의한 표고버섯과 대비하여 버섯내 함황 아미노산 및 안토시안의 조성을 공시된 방법에 따라 측정하였으며, 그 결과는 각각 하기 표 1 및 2에 나타낸 바와 같다.
Compared with the shiitake mushroom according to the comparative example, the mushroom harvested in Examples 1 to 5 was measured for the composition of the sulfamic amino acid and anthocyanin in the mushroom according to the published method, and the results are shown in Tables 1 and 2 same.

(단위 : 습물기준, mg/g)(Unit: wet basis, mg / g) 샘플Sample 시스테인Cysteine 메티오닌Methionine 실시예 1Example 1 0.1340.134 0.0110.011 실시예 2Example 2 0.1350.135 0.0100.010 실시예 3Example 3 0.1350.135 0.0100.010 실시예 4Example 4 0.1370.137 0.0110.011 실시예 5Example 5 0.1430.143 0.0200.020 실시예 6Example 6 0.0580.058 0.0670.067

(단위 : 습물기준, mg/g)(Unit: wet basis, mg / g) 샘플Sample 안토시안Anthosian 실시예 1Example 1 0.0230.023 실시예 2Example 2 0.0250.025 실시예 3Example 3 0.0240.024 실시예 4Example 4 0.0330.033 실시예 5Example 5 0.0510.051 실시예 6Example 6 0.0030.003

상기와 같이, 본 발명에 따른 톱밥배지를 이용하여 표고버섯을 재배한 결과 재배기간이 단축되고 수확량이 증가한 것 뿐만 아니라 함황 아미노산 및 안토시안 함량이 증가된 것으로부터 유황 성분과 안토시안이 버섯내로 흡수가 잘 이루어진 것을 알 수 있다.
As described above, cultivation of shiitake mushroom using the sawdust medium according to the present invention resulted in shortening of cultivation period and increase in yield, as well as increased sulfur amino acid and anthocyanin content, so that the sulfur component and anthocyanin were absorbed into the mushroom well .

상기와 같이, 본 발명의 바람직한 실시 예를 참조하여 설명하였지만 해당 기술 분야의 숙련된 당업자라면 하기의 특허청구범위에 기재된 본 발명의 사상 및 영역으로부터 벗어나지 않는 범위 내에서 본 발명을 다양하게 수정 및 변경시킬 수 있음을 이해할 수 있을 것이다.While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims. It can be understood that

Claims (7)

참나무톱밥, 탄산칼슘, 소맥피, 면실피, 흑설탕, 물, 나노제독유황 및 안토시안을 포함하는 버섯재배용 톱밥배지를 제조하는 단계; 상기 톱밥배지를 배양용기에 담고 밀봉한 후, 살균을 위해 가열하고 냉각하는 단계;.상기 냉각된 톱밥배지에 버섯 종균을 접종하는 단계; 상기 버섯 종균을 암배양을 통해 균사체를 배양하는 단계; 상기 암배양을 거친 버섯 균사체를 명배양하여 갈변화를 진행시키는 단계; 및 상기 갈변화가 완료된 버섯균사체를 포함하는 배양용기를 재배사에 입사하여 버섯으로 배양하는 단계를 포함하는 톱밥배지를 이용한 버섯의 재배방법. Preparing a sawdust medium for mushroom cultivation comprising oak sawdust, calcium carbonate, wheat bran, cotton bark, brown sugar, water, nano-decontamination sulfur and anthocyanin; The method comprising: inoculating the sawdust medium into a culture container, sealing the mixture, heating and cooling the paste medium for sterilization, inoculating the cooled sawdust medium with mushroom seeds, Culturing the mycelium by culturing the mushroom strain in an arm culture; Culturing the mushroom mycelium which has undergone the arm culturing and culturing the mushroom mycelium; And the mushroom mycelium which has undergone the change of browning is introduced into a grower and cultured in a mushroom. 제 1항에 있어서,
참나무톱밥 40~50 중량%, 탄산칼슘 0.1~1.0 중량%, 소맥피 5~10 중량%, 면실피 1~5 중량%, 흑설탕 0.1~1.0 중량%, 물 30~50 중량%, 나노제독유황 0.01~0.1 중량% 및 안토시안 0.01~0.1 중량%를 포함하는 톱밥배지를 이용한 버섯의 재배방법.
The method according to claim 1,
The present invention relates to a method for producing a fermented soybean oil, comprising the steps of: preparing a soybean oil containing 40 to 50% by weight of oak sawdust, 0.1 to 1.0% by weight of calcium carbonate, 5 to 10% by weight of wheat bran, 1 to 5% To 0.1% by weight of anthocyanins and 0.01 to 0.1% by weight of anthocyanins.
제 1항에 있어서,
피트모스 10~20 중량%를 더 포함하는 것을 특징으로 하는 톱밥배지를 이용한 버섯의 재배방법.
The method according to claim 1,
And 10 to 20% by weight of peat moss.
제 1항에 있어서,
목초액 1~5 중량%를 더 포함하는 것을 특징으로 하는 톱밥배지를 이용한 버섯의 재배방법.
The method according to claim 1,
And 1 to 5% by weight of a woodpecker. The method of cultivating mushroom using the sawdust medium.
제 1항에 있어서,
실크펩타이드 1~5 중량%를 더 포함하는 것을 특징으로 하는 톱밥배지를 이용한 버섯의 재배방법.
The method according to claim 1,
And 1 to 5% by weight of a silk peptide.
제 1항에 있어서,
피트모스 10~20 중량%, 목초액 1~5 중량% 및 실크펩타이드 1~5 중량%를 더 포함하는 것을 특징으로 하는 톱밥배지를 이용한 버섯의 재배방법.
The method according to claim 1,
A peach moss 10 to 20% by weight, a vinegar solution 1 to 5% by weight, and a silk peptide 1 to 5% by weight.
제 1항 내지 제6항 중 선택된 어느 한 항에 의한 방법에 의해 재배된 버섯.A mushroom cultivated by the method according to any one of claims 1 to 6.
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CN106105775A (en) * 2016-06-30 2016-11-16 广西仁泰生物科技有限公司 A kind of method utilizing Eucalyptus waste material and wheat straw to produce Agaricus Bisporus
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KR20220076769A (en) * 2020-12-01 2022-06-08 경상국립대학교산학협력단 Culture method for enhancing content of Cordyceps militaris mycelium using woody liquid medium and Light-Emitting Diode
KR20220076772A (en) * 2020-12-01 2022-06-08 경상국립대학교산학협력단 Culture method for enhancing cordycepin content of Paecilomyces javanicus mycelium using woody liquid medium and Light-Emitting Diode

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