KR20150118049A - Composition for preventing and treating periodontal disease comprising Nardostachys jatamansi extract as an active ingredient - Google Patents

Abstract

The present invention relates to a novel use of a Nardostachys jatamansi extract and, more specifically to a pharmaceutical composition for preventing and treating periodontal diseases comprising a Nardostachys jatamansi extract as an active ingredient. The extract can prevent periodontal diseases such as gingivitis by blocking proliferation of dental plaque pathogenic microorganisms as well as not influencing growth and development of streptococcus oralis or the like which are intraoral normal oral bacteria.

Description

TECHNICAL FIELD The present invention relates to a composition for prevention and treatment of periodontal disease comprising an extract of Ganoderma lucidum as an active ingredient,

The present invention relates to a novel use of ginseng extract, and more particularly to a pharmaceutical composition for preventing and treating periodontal disease comprising ginseng extract as an active ingredient.

Gam - sung has traditionally been used in gastric, gastrointestinal, thoracic, gastrointestinal, vomiting, headache, and other conditions. Nardostachys jatamansi , NJ) has been used extensively as a tonic, stimulant, and anticonvulsant in several Asian countries, and has also been used to treat epilepsy, pathological excitement, tremor, and seizures (Bagchi, A., et al., Planta Med. , 57, 9697 (1991)). Various sesquiterpenes such as Jatamansic acid and Jatamansone, lignans, and neo lignans are present in the roots (Chatterji, A, et al., National Institute of Science Communication, vol. 5, pp 99-100 (1997); Arora, RB., Indian Council of Medical Research, vol. 51 (1965)).

The perennial herbaceous ginseng roots of the persimmon ferns include 1-aristolene-2-one, nardostachone, 1,8,9,10-tetrad-hydroaristolan-2- one) and the like are contained. Gyeongsanghyeong self-functioning abdominal pain and thoracic pain caused by the window to relieve pain, and also applies to nervous stomach and headache. Each leg (脚气) is dipped in water to wash the lesion. It works on the central nervous system and has a sedative effect like the heel root. If the heart rate is not constant, heart rate control effect is remarkable, and smooth muscle spasm such as bronchus, small intestine, large intestine and uterus is released. Because of the efficacy of the dog's satellite diet, it controls the symptoms of malnutrition, poor appetite, and so on. It also makes fragrance with all spices. Water, which is rooted in ginseng, has been used for mental disorders, insomnia, blood disorders and circulatory system disorders (Uniyal, MR, et al., J. Res. Indian Med. 4, 83 (1969) (Li, p. Et al., Journal of Pharmacology Science, 93, 122-125 (2003)), which enhances perfusion and central nervous system (CNS) effects. In addition, alcohol extracts of ganoderma lucidum protect rats from thioacetamide induced liver injury (Bagchi, A., et al.). However, it has not yet been disclosed or disclosed in any literature regarding periodontal disease of ginseng, especially periodontitis.

On the other hand, periodontal disease is often referred to as a style, depending on the degree of disease gingivitis (gingivitis) and periodontitis (periodontitis) is divided into. It is a relatively light and fast-acting periodontal disease called gingivitis that is limited to gingiva or soft tissue, and it is called periodontitis when the inflammation progresses to the gums and the gums. There is a V-shaped gap between the gum (gums) and the teeth. Periodontal disease is caused by bacteria attacking the lower part of the gum line of this sulcus and damaging the periodontal ligament and adjacent tissues. As the inflammation progresses and more tissue is damaged, the groove develops into a periodontal pocket, and the more the periodontal disease becomes, the deeper the depth of the periodontal pocket becomes. Periodontal disease is a periodontal disease that causes inflammation and bone loss in the periodontal ligament.

Gingivitis and periodontitis are caused by gingival associations and the activity of plaque bacteria in the plaque accumulated on the tooth surface adjacent to the gingival sulcus. Germicidal microorganisms, especially in anaerobic Gram negative microorganisms, secrete toxins and proteolytic enzymes to directly destroy the periodontal tissues or react with immune cells of our body to induce the production of various immune substances, It can lead to inflammation and destruction of tissue. The teeth and periodontal tissue can be treated once they are destroyed, but they are serious in that they are not restored to their original tissue state. Therefore, the process of propagation of the pathogenic microorganisms, which are representative of these local causes, Blocking may be a method of preventing or rapidly treating oral disease.

A variety of antimicrobial agents including antibiotics having sterilization and bacteriostatic action against such microorganisms have been developed as inhibitors and remedies for cavities, periodontal diseases or gingivitis. Conventional materials include sodium copper chlorophyllin, sodium fluoride or benzethonium chloride,

(Benzethonium chloride), aromatic carboxylic acids including benzoic acid and the like, allantoin, tocopherol acetate, antiplasmic agents, and antibiotics. However, in the case of the fluorine-chlorine-containing compound, safety of the human body is problematic. Antibiotics can cause systemic side effects, including diarrhea and vomiting, in the whole body, It is difficult to use it for a long period of time because it can induce homozygosity, so that it can be used only as a therapeutic agent. In addition, in the case of treatment by surgical treatment or mechanical therapy, there is a disadvantage that treatment is not easy. Therefore, it is easy to prevent or treat as compared with surgical therapy or mechanical therapy, and there is no problem of tolerance or stability such as antibiotics even when used for a long time, and there is no problem of side effects such as a compound containing fluorine and chlorine components There is an urgent need to develop a composition for the prevention or treatment of oral diseases derived from natural materials, and there is an increasing demand for developing a composition capable of preventing or treating oral diseases from natural materials. On the other hand, natural substances such as green tea extract and yacon extract are known in this connection. However, development of more effective substances is continuously required.

Accordingly, the inventors of the present invention have completed the present invention by confirming that ginseng root extract is an effective substance for the prevention and treatment of periodontal disease while conducting herbal medicine studies on ginseng root.

In order to solve the above problems, the present invention aims to inhibit the development of periodontal disease such as gingivitis by inhibiting the propagation of pathogenic microorganisms in the oral cavity by containing the ginseng extract as active active ingredient.

In order to accomplish the above object, the present invention provides a composition for preventing and treating periodontal disease, comprising an extract of ginseng root as an active ingredient.

The periodontal disease may be selected from the group consisting of Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans) ). ≪ / RTI >

Alternatively, the periodontal disease is caused by Prevotella intermedia.

The ginseng root extract is preferably obtained by extracting roots of ginseng root with water or an organic solvent, and then extracting the extract from a hexane fraction layer or an ethyl acetate fraction layer.

And, the composition may further comprise a pharmaceutically acceptable carrier.

The present invention may take the form of a food composition comprising the composition.

Hereinafter, the present invention will be described in detail.

The ginseng extract of the present invention can be applied to any part of the ginseng line, but is preferably extracted from the root. The extraction solution can be obtained by extraction with water or an organic solvent. Examples of the organic solvent include lower alcohol, acetone, chloroform, methylene chloride, ether, ethyl acetate, hexane and the like. As the lower alcohol, methanol, ethanol, propanol and butanol can be mentioned, and ethanol is preferable.

When water is used, 1 to 20 times, preferably 5 to 15 times, more preferably 10 times, water is added to the ginseng root dried powder or powder and heated at 80 to 100 ° C for 1 to 24 hours, preferably For 2 to 6 hours, more preferably for 2 hours, and then filtered to produce a hot-water extract of Rosemary root. In the case of the organic solvent extract, 1 to 5 times, preferably 3 times, of the organic solvent is added to the dried or powdered gherkin-like product or powder, and the mixture is heated at room temperature (20 to 30 ° C) or warmed for 10 to 100 hours, 40 hours, more preferably 24 hours, followed by filtration and concentration under reduced pressure. In the above extraction methods, the extraction process may be repeated twice or more as necessary, and the extract obtained after filtration may be prepared into a powder form by lyophilization, spray drying or vacuum drying.

The ginseng root extract of the present invention is useful for periodontal diseases such as periodontitis and gingivitis. Pathogenic microorganisms causing periodontitis include, but are not limited to, Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans ), And they are effective against periodontitis caused by them. An extract of ginseng extract is effective against diseases caused by Prevotella intermedia, a typical microorganism causing gingivitis. More than 300 microorganisms are known in the mouth of a person. When a tooth is formed on the surface of a tooth, pathogenic microorganisms form an acid by using the sugar introduced into the oral cavity. This acid decarboxylates the main component of the tooth, . Anaerobic Gram-negative microorganisms secrete toxins and proteolytic enzymes, directly destroying periodontal tissue, or react with immune cells to produce inflammatory cytokines, which can lead to inflammation and destruction of periodontal tissue. Thus, gingivitis and periodontitis may be a complex action of multiple, more pathogenic microorganisms. However, since the main mechanism is thought to be similar, the ginseng root extract of the present invention is considered to be useful for periodontal disease.

On the other hand, ginseng extract has antimicrobial effect against various microorganisms such as various gram-positive and negative bacteria and yeast. Especially, the hexane fraction layer of hot water extract showed good antimicrobial activity. And, the ethyl acetate fraction was weaker than the hexane fraction but showed some antimicrobial activity.

The term "pharmaceutically acceptable carrier" refers to a pharmaceutically acceptable material, such as a liquid or solid filler, diluent, excipient or solvent, which serves to transport the active ingredient from one organ or portion of the body to another organ or portion of the body. , Composition or vehicle. The composition of the present invention can be prepared by adding a pharmaceutically acceptable carrier together with at least one pharmaceutically acceptable carrier. The term "pharmaceutically acceptable" as used herein means physiologically acceptable and does not normally cause an allergic reaction or a similar reaction when administered to humans. Such carriers include, but are not limited to, saline, buffered saline, water, glycerol, and ethanol, and any suitable formulation known in the art (Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA) .

The pharmaceutical composition for the preparation of the medicinal herb extract of the present invention can be administered orally at the time of clinical administration and can be used in the form of a general medicinal preparation. In the case of formulation, a filler, an extender, a binder, a wetting agent, , A surfactant, and the like. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like. Examples of liquid formulations for oral use include suspensions, solutions, emulsions and syrups. Common diluents such as water, In addition to liquids and paraffins, various excipients such as wetting agent sweetening agents, perfumes, preservatives and the like may be included.

In addition, the herbal medicine which may be added to the composition of the present invention may be any pharmaceutically acceptable herbal medicine, for example, Angelicae tenuissimae Radix, Gastrodiae Rhizoma, Bapleuri Radix, (Rhizoma), Angelicae gigantis Radix, Persicae Semen, Cinnamomi Ramulus, Rhei Rhizoma, Glycyrrhizae Radix, Cnidii Rhizoma, Aurantii nobilis Pericarpium, Alismatis Rhizoma, Coptidis Rhizoma, Scutellariae Radix, Hoelen, Paeoniae Radix, Atractylodis Rhizoma alba, Phellodendri Cortex, Gardeniae Fructus, Pinelliae Tuber, Uncaria Ramuluset, Uncus, Ponciri Fructus, Ginseng, Liriopis Tuber, Polygalae Radix, Acori graminei Rhizoma, Atractylodis Rhizoma alba, Chrysanthemi Flos, Ledebouriellae Radix, ), Ginger (Zingiberis Rhizoma crudus), ganoderma (Natrii sulfas), control yphi Fructus, Salviae Radix, Mautan Radicis Cortex, Rehmanniae Radix, Menthae Herba, Dioscoreae Rhizoma, Polyporus, Polygonimultiflori Radix, Allii tuberosi Semen, Cassiae Semen, Lycii Fructus, Araliae cordatae Radix, Eucommiae Cortex, Hedyotis Herba, Saururus Herba, Artemisiaecapillaris Herba, Anemarrhenae, Rhizoma, Rhizoma, Carthami Flos, Astragali Radix, Lycopodium, Ginkgonis Folium, Polygonati Rhizoma, Nelumbinis Semen, Fossilia ossis Mastodi, Lycii radicis Cortex ), Achyranthis Radix, Rehmanniae Radix preparata, Perillae Semen, Thujae Semen, Hordei Fructus germinatus, Cuscutae Semen, Morindae Radix, Pini koraiensis Radix) may be used alone or in combination.

The compositions of the present invention may be administered orally or parenterally. Parenteral administration methods include, but are not limited to, intravenous, intramuscular, intraarterial, intramedullary, intrathecal, intracardiac, transdermal, subcutaneous, intraperitoneal, intranasal, enteral, topical, sublingual or rectal administration Lt; / RTI > Preferably, the composition of the present invention can be administered transdermally. The term " transdermal administration " as used herein refers to administration of the composition of the present invention to cells or skin to allow the active ingredient to be delivered into the skin. In the case of a preparation for oral administration, the composition of the present invention may be formulated into a powder, a granule, a tablet, a pill, a sugar, a tablet, a liquid, a gel, a syrup, a slurry, . For example, an oral preparation can be obtained by combining the active ingredient with a solid excipient, then milling it, adding suitable auxiliaries, and then processing the mixture into a granular mixture. Examples of suitable excipients include sugars including lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol and maltitol, and starches including corn starch, wheat starch, rice starch and potato starch, Cellulose such as methylcellulose, sodium carboxymethylcellulose and hydroxypropylmethyl-cellulose and the like, fillers such as gelatin, polyvinylpyrrolidone and the like. In addition, crosslinked polyvinylpyrrolidone, agar, alginic acid, or sodium alginate may optionally be added as a disintegrant. Further, the pharmaceutical composition of the present invention may further comprise an anti-coagulant, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent and an antiseptic agent.

In the case of a preparation for parenteral administration, it can be formulated by a method known in the art in the form of injection, cream, lotion, external ointment, oil, moisturizer, gel, aerosol and nasal aspirate. These formulations are described in Remington's Pharmaceutical Science, 15th edition, 1975. Mack Publishing Company, Easton, Pennsylvania 18042, Chapter 87: Blaug, Seymour, a commonly known formulary for all pharmaceutical chemistries.

The formulations of the present invention may be applied differently depending on the age, sex, condition of the subject, the degree of absorption of the active ingredient in the body, the rate of inactivation and the rate of excretion, and the drugs used in combination. The present invention also includes formulations of dosage units. The formulations are presented in separate dosage forms, such as tablets, coated tablets, capsules, pills, suppositories, and ampoules, and the content of active compound in the drug is a fraction or multiple of the individual dosage. Dosage units may contain, for example, 1, 2, 3 or 4 times, or 1/2, 1/3 or 1/4 times the individual doses. The individual doses preferably contain amounts in which the active compound is administered in a single dose, which usually corresponds to the full, half, one-third or one-fourth of the daily dose.

As used herein, the term "extract " means an active ingredient isolated from a natural product. The extract can be obtained by an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes an extract, a dry powder thereof, or all the forms formulated with it. Substances which are obtained by extracting with water or an organic solvent, then suspended in the extract, and then fractionated using hexane, chloroform, butanol, ethyl acetate or the like are also included in the scope of the extract of the present invention. Particularly, in the present invention, after extraction with hot water, hexane and ethylacetate fraction materials showed various antimicrobial activities against various microorganisms such as gram-positive and negative bacteria and yeast.

In addition, the ginseng extract according to the present invention can be provided in the form of a food composition for the purpose of preventing and improving periodontal disease. The food composition of the present invention includes all forms such as functional food, nutritional supplement, health food and food additives. Food compositions of this type may be prepared in a variety of forms according to conventional methods known in the art.

For example, as a health food, the ginseng extract extract of the present invention itself can be prepared in the form of tea, juice and drink and then consumed for drinking, granulated, encapsulated and powdered. In addition, it can be prepared in the form of a composition by mixing together the ginseng extract of the present invention and a known substance or active ingredient known to be effective for the prevention and improvement of periodontal disease.

Functional foods also include beverages (including alcoholic beverages), fruits and their processed foods (e.g., canned fruits, bottled, jam, maalmalade, etc.), fish, meat and processed foods such as ham, Etc.), breads and noodles (eg udon, buckwheat noodles, ramen noodles, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, Retort food, frozen food, various kinds of seasonings (e.g., miso, soy sauce, sauce, etc.) by adding the ginseng extract of the present invention.

Further, in order to use the reducing agent of the present invention in the form of a food additive, it may be used in the form of powder or concentrate.

In the present invention, by containing the ginseng extract as active active ingredient, it inhibits the propagation of phytopathogenic microorganisms without affecting the growth of Streptococcus arales, which is a normal oral microorganism in the oral cavity, and suppresses the occurrence of periodontal disease such as gingivitis can do.

Fig. 1 shows the measurement results of IL-6 when the extract of the present invention was treated.
Fig. 2 shows the measurement results of IL-1? When the extract of the present invention was treated.

Hereinafter, the present invention will be described in more detail based on the following examples. It should be noted, however, that the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The present invention is not limited to the following examples. Will be apparent to those skilled in the art to which the present invention pertains.

< Gam-cho  Preparation of Extract>

Three kilograms of Gamsohyang (Chinese origin, root and stem) purchased from Seoul Medicinal Plant were dried and pulverized for 5 days at shade and room temperature. The pulverized ginseng slices were washed with distilled water for about 2 hours. The extract was frozen at -80 DEG C, and then a freeze-dried powder was prepared.

In addition, 30% ethanol was used instead of hot water, and a lyophilized powder was similarly prepared.

< Gam-cho  By extract Acute toxicity  Test>

Although the ginseng root used in the present invention is widely used as a medicament, it was considered that there would be no problem in stability, but the toxicity test was conducted during oral administration and intraperitoneal administration to confirm this.

Acute toxicity tests were carried out using 6-week-old SPF SD rats. The ginseng root extract of Example 1 of the present invention was suspended in a 0.5% methylcellulose solution and administered to a 2-group animal at a dose of 5 g / kg in a single oral dose. After the administration of the test substance, the mortality, clinical symptoms, and weight changes of the animals were observed, and hematological tests and blood biochemical tests were carried out, and autopsy was performed to observe the abnormalities of the abdominal organs and thoracic organs. As a result of the test, there were no clinically symptomatic or dead animals in all the animals to which the test substance was administered, and no toxic change was observed in weight change, blood test, blood biochemical test, and autopsy findings. These results sense songhyang extract does not represent all the changes in toxicity in rat up to 5 g / ㎏ was determined orally minimum lethal dose (LD ₅₀₎ is to be a safe substance less than 5 g / ㎏.

<Antibacterial test for periodontitis>

The types and characteristics of periodontal bacteria are shown in Table 1. In addition, Streptococcus aralis , a normal oral bacterium, was also tested.

Strain Culture medium Culture characteristics Porphyromonas
gingivalis Brucella broth supplemented with yeast extract + hemin + menadione gram (-) / anaerobic Tannerella
forsythia Brucella broth supplemented with yeast extract + hemin + menadione + N-acetylmuramic acid + fetal bovine serum gram (-) / anaerobic Fusobacterium
nucleatum Brucella broth supplemented with yeast extract + hemin + menadione gram (-) / anaerobic Actinobacillus actinomycetemcimitans Brucella broth supplemented with yeast extract + 10% serum gram (-) / anaerobic

The antimicrobial activity of periodontal bacteria was assayed using the minimum inhibition concentration measurement using microdilution method. For the periodontal bacteria and the normal oral bacteria, the microorganisms and the sample were incubated in a liquid medium, and the antibacterial activity was measured by measuring the absorbance at 600 nm using a UV spectrometer to measure the viability of the cells.

Ml, 1 mg / ml, 2 mg / ml, 4 mg / ml, 8 mg / ml, 16 mg / ml and 32 mg / ml To the culture medium for each microorganism. 100 [mu] L of the culture medium to which the extract was added was added at a concentration of 1 x 10 &lt; ⁶ &gt; CFU / ml, and the mixture was incubated at 37 [deg.] C for 48 hours under anaerobic conditions.

As a result, the bacterial growth of the plaque pathogenic microorganism, Table 1, was suppressed, but the streptococcus arales, which is a normal oral bacteria, was hardly affected. The minimum inhibitory concentration was about 0.5 to 1 mg / ml, though slightly different, and more than 90% inhibition was more effective than 2 mg / ml.

&Lt; Anti-

In gingival epithelial cells (YD-38 cell, KCLB No. 60508, human lower gingival epithelial cell), Prevotella induced inflammation by stimulation of intermedia . Dissect the YD-38 cells in a 24-well dish at 1.0 × 10 ⁵ per well and incubate overnight. The culture medium is exchanged with assay medium (2% FBS medium) supplemented with ginseng root extract and related reagents and incubated for 3 h. Pre-treated cells are treated with P. intermedia at a concentration of 0.1% to induce an inflammatory response, and the medium is collected after 24 h. The concentration of IL-8 in the collected medium was analyzed using a human CXCL8 / IL-8 Duoset (R) kit (DY208, R & D Systems, USA). The details of the experiment were followed by the protocol provided by the manufacturer.

As a result of IL-8 measurement using immunofluorescence staining, the secretion of IL-8 was suppressed as in ELISA. That is, the inflammatory response was reduced.

In the hunman gingival fibroblast cell line (HGF-1), the gingivitis-inducing bacteria Prevotella Intermedia and LPS stimulated the inflammatory response. HGF-1 was cultured in DMEM medium supplemented with 20% FBS. The culture conditions of Prevotella intermedia were TSA with 5% defibrinated Sheep Blood + 5.0 mg / L (final) with 10% FBS + 5.0 mg / L (final) hemin soluttion + 1.0 mg / L (final) menadione. Add HGF to a 24-well plate at 1.0 × 10 ⁵ per well and incubate overnight. The ginseng root extract is named WSY-1, the hot water extract is designated WSY-1W, and the 30% ethanol extract is designated WSY-1E. The WSY-1W and WSY-1E samples are exchanged with DMEM medium (with 2% FBS) and incubated for 3 h for pre-treatment. Prevotella intermedia at a concentration of 0.1% (1 ul / 1000ul), and LPS (1 ug / mL), cultured for 1 hour, and cytokine (IL-6, IL-1beta) was measured after -80 ° C. W_0.25 represents 0.25 mg / ml as a hot water extract, E_0.25 represents 0.25 mg / ml as a 30% ethanol extract, NC (non-treatment) has no treatment and LPS + , 0.1% of P. i +, and 1 μg / mL of LPS and 0.1% of Prevotella intermedia . Cytokines were measured using an ELISA kit product of the R & D System, which is shown in FIGS. 1 and 2. It is confirmed that both IL-1? And IL-6 are reduced by the composition of the present invention.

< Prevotella intermedia For WSY -1 W, WSY -1E MIC (minimum inhibitory concentration) measurement>

Prevotella The cultures were incubated in TSB with 10% FBS + hemin + menadione medium for 3 days (37 ° C, anaerobic condition) and cultured in WSY-1W or 5% sheep blood + WSY-1E was diluted in the culture medium and diluted to 100 μl per well. 100 μl of each of the wells were diluted to a concentration of OD _{600 nm} = 0.1, and the mixture was incubated for 48 hours (37 ° C., anaerobic condition). The absorbance was measured by a UV spectorophotometer to measure the viability of the cells (OD _{600 nm} ). The results are as follows. Table 2 is for WSY-1W (hot water extract of ginseng root) and Table 2 is for WSY-1E (30% ethanol extract of ginseng root).

In the case of the hot-water extract, inhibition of the growth of the gingival sulphate strain can be confirmed at a concentration of WSY-1_W0.25 mg / ml or more. However, in the case of excess, different results are shown. 30% ethanol extract showed inhibition of the growth of gingival fungus within a certain range, but it did not show consistent results, and excessive amount of cytotoxicity seems to be necessary and additional experiments and studies are needed .

On the other hand, it is considered that not only Prebotella intermedia but also other pathogenic microorganisms mentioned above are considered to be similar and thus, the effect is likely to be effective. In addition, the effect of the hexane fraction layer of the hot-water extract was better, and the ethyl acetate fraction was also less effective than the hexane fraction layer, but showed excellent results.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the scope of the present invention as defined by the appended claims. It should be understood that it can be performed within a range.

Claims (6)

A composition for preventing and treating periodontal disease, comprising an extract of ginseng root as an active ingredient. The method according to claim 1,
The periodontal disease may be selected from the group consisting of Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans. Lt; RTI ID = 0.0 &gt; and / or &lt; / RTI &gt; The method according to claim 1,
Wherein the periodontal disease is caused by Prevotella intermedia. The method according to claim 1,
Wherein the ginseng root extract is obtained by extracting ginseng root with hot water or an organic solvent. 5. The method of claim 4,
Wherein the extract is extracted with hot water or an organic solvent and is obtained from a hexane fraction layer or an ethyl acetate fraction layer of the extract. A health functional food for preventing or ameliorating periodontal disease, comprising a composition according to any one of claims 1 to 4, including a food-acceptable food supplementary additive, as an active ingredient.

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