KR20140084502A - For improving liver function with aloe and Curcuma longa and A method of manufacturing the same and Using the formulations for improving liver function - Google Patents
For improving liver function with aloe and Curcuma longa and A method of manufacturing the same and Using the formulations for improving liver function Download PDFInfo
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- KR20140084502A KR20140084502A KR1020120154000A KR20120154000A KR20140084502A KR 20140084502 A KR20140084502 A KR 20140084502A KR 1020120154000 A KR1020120154000 A KR 1020120154000A KR 20120154000 A KR20120154000 A KR 20120154000A KR 20140084502 A KR20140084502 A KR 20140084502A
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- aloe
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- liver function
- improving liver
- fermented product
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
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- Tropical Medicine & Parasitology (AREA)
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- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
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Abstract
Description
본 발명은 간 기능을 개선시키는 발효물에 관한 것으로, 상세하게는 알로에와 울금로 이루어지는 혼합배지(기질)에 버섯종균을 접종한 후 배양 및 발효시켜 간 기능 개선용 혼합발효물을 획득하고, 이 혼합발효물의 추출물을 유효성분으로 하는 간 기능 개선용 제제에 관한 것이다.
The present invention relates to a fermentation product which improves liver function. More specifically, the present invention relates to a fermentation product obtained by inoculating a mushroom strain into a mixed medium (substrate) comprising aloe and corn, followed by cultivation and fermentation to obtain a mixed fermentation product for improving liver function, The present invention relates to a preparation for improving liver function comprising an extract of a mixed fermented product as an active ingredient.
본 발명에 사용된 울금은 생강과에 속한 여러해살이풀이고, 타원형의 잎이 뿌리에서 나고 줄기는 노란색을 띠며 여름과 가을에 입술 모양의 노란색 꽃이 핀다. 땅속줄기인 덩이뿌리는 양념이나 염료, 흥분제와 같은 의약품으로 이용된다. 황색 색소 쿠르쿠민 및 정유를 함유하며 간이나 담도 질환 및 지혈제로 사용되는 열대 아시아에 자생하는 다년초 Curcuma longa L.의 근경이며, 우리나라의 경우 제주도 및 진도지방에서 생산되어진다.The perennials used in the present invention are perennial plants belonging to the ginger family, oval-shaped leaves arising from the roots, yellow-stalked and yellow-lipped flowers in summer and autumn. The roots of root are used as medicines such as spices, dyes and stimulants. It is a rhizome of Curcuma longa L., a perennial plant native to tropical Asia, containing yellow pigmented curcumin and essential oils and used as a liver and biliary disease and hemostatic agent. In Korea, it is produced in Jeju and Jindo provinces.
울금에 대한 연구로는 (KR)2011-0069975(공개, 경희대학교 산학협력단)은 강황 추출물을 포함하는 전립선 비대증의 치료 또는 예방을 위한 약제학적 조성물에 대한 연구, (KR)2011-0062231(공개, 군산시)은 강황에서 추출되는 정유를 함유한 구강청정제 및 그 제조 방법에 대한 연구, (KR)2011-0059189(공개, 한국생명공학연구원)은 온울금으로부터 추출된 정유를 유효성분으로 함유하는 염증성 질환의 예방 및 치료용 조성물에 대한 연구, (KR)2011-0053590(공개, 한국인스팜(주))은 발효울금 추출물을 함유한 알코올성 간질환의치료 및 예방용 조성물에 대한 연구, (KR)2011-0038531(공개, 서원대학교산학협력단 외)은 상황버섯 - 울금 발효액을 함유하는 숙취해소용 조성물에 대한 연구, (KR)2011-0004763(공개, 한국생명공학연구원)은 울금 추출물을 포함하는 인플루엔자 바이러스 감염의 예방 및 치료용 조성물 및 뉴라미니데이즈 활성의 억제용 조성물에 대한 연구, (KR)2010-0114266(공개, 전남대학교산학협력단)은 체중증가의 방지 및 혈중 중성지질의 감소를 위한 울금 메탄올 추출물을 함유하는 식이 조성물에 대한 연구, (KR)2010-0100455(공개, 한국콜마 외 1)은 피부 진균증에 유효한 온울금 오일과 이를 포함하는 화장료 조성물에 대한 연구, (KR)2010-0086411(공개, 김선옥 외1)은 혈행개선 및 혈관확장 기능을 가지는 피부 도포용 조성물에 대한 연구, (KR)2007-0022403(공개, (주)태영이앤티)은 유산균을 이용한 발효 강황 조성물 및 그 제조 방법에 대한 연구, (KR)2002-0073847(공개, (주)바이오시너젠)은 쿠르쿠민 또는 울금 추출물을 포함하는 치매 예방 및 치료용 조성물에 대한 연구, (KR)10-0824615(등록, 동국대학교 산학협력단)은 울금 추출물을 함유하는 동맥경화 및 고혈압의 예방 및 치료용 약학 조성물에 대한 연구, (KR)10-0506427(등록, (주)뉴로넥스)은 강황 추출물을 함유한 위염, 위궤양 예방을 위한 건강보조식품에 대한 연구 등이 이루어져 있다.(KR) 2011-0069975 (published by Kyung Hee University Industry & Academy Collaboration Foundation) is a pharmaceutical composition for the treatment or prevention of hyperplasia of prostate, including turmeric extract, (KR) 2011-0059189 (published by Korea Research Institute of Bioscience and Biotechnology) is a research institute for the research and development of an oral cleaning agent containing essential oil extracted from turmeric and an inflammatory disease (KR) 2011-0053590 (published by Korea Inspam Co., Ltd.), a study on compositions for the treatment and prevention of alcoholic liver diseases containing fermented koi extract, (KR) 2011 -0038531 (published by Seowon University Industry & Academy Collaboration Foundation), Research on composition for hangover disease containing mushroom-ugum fermentation broth, (KR) 2011-0004763 (published by Korea Research Institute of Bioscience and Biotechnology) feeling (KR) 2010-0114266 (published by Chonnam National University Industry & Academy Collaboration Foundation) has been investigated for a composition for preventing and treating hyperlipidemia and for the inhibition of neuraminidase activity, (KR) 2010-0086411 (published by Kim, Sun-ok, et al.), (KR) 2010-0100455 (KR) 2007-0022403 (published by Taeyoung Lee < RTI ID = 0.0 > Ltd.), < / RTI > is a study of a composition of fermented sulfuric acid using lactic acid bacteria and a method for preparing the composition, (KR) 2002-0073847 (published by Bio Synergen Co., Ltd.) is a research for the composition for the prevention and treatment of dementia including a curcumin or uroguin extract, (KR 10-0824615 (registered by Dongguk University) Extract-containing (KR) 10-0506427 (Registered by Neuronex Co., Ltd.) is a pharmaceutical composition for prevention and treatment of arteriosclerosis and hypertension, including gastric juice containing a turmeric extract and a study on health supplements for the prevention of gastric ulcers consist of.
또한, 알로에는 식물학상으로 백합과의 알로에 속에 속하는 다년생초이며, 약 4천여년간 민간 및 한방약으로 애용되어온 최고의 식물로 일컬어지고 있다. In addition, aloe is a botanically perennial plant belonging to the genus Aloe of the lily, and has been regarded as the best plant that has been used as a private and herbal medicine for about 4,000 years.
많은 알로에 종류 가운데 현재 생즙을 직접 식용으로 사용하는 알로에로는 알로에 베라, 알로에 아보레센스, 사포나리아 등 3 ~ 4종이 있으며, 알로에가 가지는 주요성분으로는 고분자 다당체, alomichin, aloin, aloetic acid, aloe ulcin 등이 있어 고혈압 저하, 항종양, 건위정장작용, 세포재생작용 등의 효능 및 효과를 나타낸다. Among many aloe varieties, aloe vera, aloe vera senes, saponaria and other three or four species of aloe which are currently used for direct edible use, include aloe vermiculite, alomin, aloin, aloe ulcin and the like, and exhibit the efficacy and effects such as hypertension deterioration, antitumor, dryness regeneration, and cell regeneration.
상기와 같이 울금과 알로에를 각각 이용한 다양한 기술이 제공되어 있다.As described above, a variety of techniques using Ulema and Aloe are provided.
그러나 본 발명과 같이 알로에와 울금을 함께 이용하여 효과를 향상시킨 간 기능 개선용 소재는 아직 개발되어 있지 않다.However, as in the present invention, a material for improving liver function, in which the effects of Aloe and Uleum are used together, has not yet been developed.
또한, 본 발명과 같이 울금과 알로에로 이루어진 천연물 혼합배지에 버섯을 배양/발효시켜 간 기능 개선효능을 갖는 소재에 대한 연구도 없는 실정이다.
Further, as in the present invention, there is no research on a material having liver function improving effect by cultivating / fermenting mushroom in a natural material mixed medium consisting of Ulgus and Aloe.
본 발명은 상기와 같이 종래기술로 제시하고 있지 않은 울금과 알로에를 이용하여 간 기능을 개선 시킬 수 있는 발효물과 이의 제조방법 및 발효물의 추출물을 유효성분으로 하는 제제를 제공하고자 하는 것이다. The present invention is to provide a fermented product capable of improving liver function by using corn and aloe, which is not proposed in the prior art as described above, a process for producing the fermented product, and a preparation containing the extract of the fermented product as an active ingredient.
즉, 본 발명에서는 울금과 알로에로 이루어진 천연물배지로 버섯을 배양 및 발효시켜 간기능 개선용 제제에 사용되는 혼합 발효물과 이의 제조방법을 제공하는 목적과, 상기 혼합 발효물의 추출물을 유효성분으로 함유하는 간 기능을 개선시킬 수 있는 제제를 제공하는데 목적이 있다.
That is, the object of the present invention is to provide a mixed fermented product for use in a preparation for improving liver function by culturing and fermenting mushroom with a natural material medium consisting of ganoderma lucerum and aloe, and a process for producing the same, Which is capable of improving liver function.
상기와 같은 목적을 달성하고자 본 발명인 알로에와 울금을 이용한 간 기능 개선용 혼합발효물은 알로에와 울금으로 이루어지는 천연물 혼합배지에 버섯종균을 발효시켜 획득되며, 상기 천연물 혼합배지는 생엽을 이용한 알로에 70~99중량%와, 울금 1~30중량%의 비율로 혼합하고 이를 멸균처리한 혼합물로 이루어지며, 상기 알로에는 보통 알로에 베라를 사용한다.In order to achieve the above-mentioned object, the mixed fermented product for improving liver function using aloe and uroguine according to the present invention is obtained by fermenting mushroom seeds in a natural product mixed medium comprising aloe and cucumber, 99% by weight, and Ulgus at a ratio of 1 to 30% by weight, and sterilized. The aloe is usually aloe vera.
본 발명인 알로에와 울금을 이용한 간 기능 개선용 혼합발효물의 제조방법은, 알로에와 울금으로 이루어지는 천연물 혼합배지에 버섯종균을 발효시켜 간 기능 개선용 혼합발효물을 획득함을 특징으로 하는 것으로, 알로에와 울금을 혼합하여 가열멸균한 후 급랭하여 천연물 혼합배지를 제조하는 배지준비단계(S1)와; 상기 배지준비단계(S1)에서 준비된 천연물 혼합배지에 버섯종균을 접종하는 접종단계(S2)와; 상기 접종단계(S2)에서 버섯종균을 접종한 천연물 혼합배지를 온도 22~30℃에서 4~10일 동안 배양 및 발효시키는 발효단계(S3)를 포함한다.The method for producing a mixed fermented product for improving liver function using aloe and uroguine according to the present invention is characterized by obtaining a mixed fermented product for improving liver function by fermenting mushroom seeds in a natural product mixed medium comprising aloe and cucumber, (S1) for preparing a natural material mixed medium by mixing and heating sterilized water after quenching and quenching; A seeding step (S2) of inoculating the mushroom seedlings into the natural material mixed medium prepared in the medium preparation step (S1); And a fermentation step (S3) of cultivating and fermenting the natural material mixed medium inoculated with mushroom seeds at 22 to 30 ° C for 4 to 10 days in the seeding step (S2).
상기 배지준비단계(S1)에서 천연물 혼합배지는 알로에 70~99중량%와, 울금 1~30 중량%으로 혼합하여 발효 배양조에 투입하여 113~125℃로 10~40분간 가열하여 멸균처리하고, 20~25℃로 급랭 처리하여 이루어진다.In the medium preparation step (S1), the natural material mixture medium is mixed with 70 to 99% by weight of aloe and 1 to 30% by weight of corn, and the mixture is put into a fermentation culture tank and sterilized by heating at 113 to 125 ° C for 10 to 40 minutes, To < RTI ID = 0.0 > 25 C. < / RTI >
본 발명인 간 기능 개선용 제제는 상기와 같은 알로에와 울금을 이용한 간 기능 개선용 혼합발효물 및 그 제조방법으로 획득되어지는 간 기능 개선용 혼합발효물로부터 추출물을 획득하고, 이를 유효성분으로 함유하는 간 기능을 개선용 제제를 특징으로 한다.The agent for improving liver function of the present invention is obtained by obtaining an extract from a mixed fermented product for improving liver function using the above aloe and corn and the mixed fermented product for improving liver function obtained by the method for producing the same, Characterized by an agent for improving liver function.
상기에서 사용되는 버섯 종균은 영지버섯(Ganoderma lucidum), 상황버섯(Phellinus linteus), 붉은 동충하초(Cordyceps militaris ), 누에 동충하초(Paecilomyces japonica), 노루궁뎅이버섯(Hericium erinaceum), 아가리쿠스 브라제(Agaricus blazei)으로 이루어진 군으로부터 1종을 선택하여 단독사용되며, 상기 버섯 종균은 온도 22~30℃, 통기량은 0.3~0.8vvm으로 4~8일간 배양한 버섯종균을 사용한다.
The mushroom seeds used in the above are ganoderma lucidum ), mushroom ( Phellinus linteus ), red cordyceps ( Cordyceps militaris , Paecilomyces japonica , Wherein the mushroom strain is selected from the group consisting of Hericium erinaceum and Agaricus blazei , and the mushroom strain is used at a temperature of 22 to 30 ° C and aeration amount of 0.3 to 0.8 vvm for 4 to 8 days Cultivated mushroom seeds are used.
상기와 같이 알로에와 울금으로 이루어진 천연물 혼합배지를 기질로 하여 버섯을 발효시킨 획득한 혼합발효물은 간 기능 개선효능분야 중 알코올 분해 및 숙취해소 능력이 우수하며, 관능적인 측면에서도 발효 전(前)인 천연물 혼합물보다 우수하므로 간 기능개선 목적에 매우 유용한 소재로 사용될 수 있다.As described above, the obtained mixed fermented product obtained by fermenting mushroom using a natural material mixed medium consisting of aloe and urea as a substrate has excellent ability of decomposing alcohol and hanging out in the field of improving liver function, Which is very useful for the purpose of improving liver function.
특히 알로에와 울금으로 이루어진 천연물 혼합배지를 이용한 혼합발효물은 울금이나 알로에가 가지는 각각의 특유한 강한 맛에 비하여 부드러운 특성이 있어 직접 또는 간접적으로 식품의 원료로 활용이 가능할 것이며, 효과도 클 것으로 기대된다. In particular, the mixed fermented product using a natural material mixed medium consisting of aloe and urea has a soft characteristic as compared with the characteristic strong taste of each of corn and aloe, so that it can be used directly or indirectly as a raw material of food and its effect is expected to be great .
도 1은 본 발명의 바람직한 실시예를 나타낸 알로에-울금으로 이루어진 천연혼합물을 천연물 혼합배지로 하여 간 기능 개선에 효과가 있는 간 기능 개선용 혼합발효물을 획득하는 방법의 블록도.
도 2는 본 발명의 실시예인 알로에-울금으로 이루어진 천연혼합물을 천연물 혼합배지로 하여 획득한 혼합발효물의 ADH의 활성측정비교표.
도 3은 본 발명의 실시예인 알로에-울금으로 이루어진 천연혼합물을 천연물 혼합배지로 하여 획득한 혼합발효물의 ALDH의 활성측정비교표. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a block diagram of a method for obtaining a mixed fermented product for improving liver function, which is effective for improving liver function, using a natural mixture of aloe-ugum as a preferred embodiment of the present invention.
FIG. 2 is a comparison chart of ADH activity measurement of mixed fermented products obtained by using a natural mixture made of aloe-ugum, which is an embodiment of the present invention, as a natural material mixed medium.
FIG. 3 is a comparative table for measuring the activity of ALDH in a mixed fermented product obtained by mixing a natural mixture of aloe-ugum, which is an embodiment of the present invention, with a natural material mixture.
첨부된 도면을 참조로 본 발명의 바람직한 실시예를 상세히 설명하도록 한다. 이에 앞서, 본 명세서 및 청구범위에 사용된 용어나 단어는 통상적이거나 사전적인 의미로 한정해서 해석되어서는 아니되며, 발명자는 그 자신의 발명을 가장 최선의 방법으로 설명하기 위해 용어의 개념을 적절하게 정의할 수 있다는 원칙에 입각하여 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 한다.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Prior to this, terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms, and the inventor should appropriately interpret the concepts of the terms appropriately It should be interpreted in accordance with the meaning and concept consistent with the technical idea of the present invention based on the principle that it can be defined.
울금과 알로에로 이루어지는 천연물 혼합배지에 버섯 종균을 접종하여 발효시켜 획득되는 울금-알로에 천연혼합발효물(이하, '혼합발효물'이라 함)은 아래와 같은 방법으로 제조된다.A natural fermented product of Ulleung-Aloe (hereinafter referred to as "mixed fermented product") obtained by fermenting mushroom seedlings by inoculating a natural material mixed medium consisting of corn and aloe is produced by the following method.
천연물 혼합배지로 한 혼합발효물의 제조방법은 크게 천연물 혼합배지를 제조하는 배지준비단계(S1)와, 상기 천연물 혼합배지에 배양할 버섯종균을 준비하는 버섯종균준비단계(S1')와, 상기 천연물 혼합배지에 버섯종균준비단계(S1')에서 준비된 버섯종균을 접종하는 접종단계(S2)와, 상기 버섯종균을 접종한 천연물 혼합배지를 배양 및 발효시키는 발효단계(S3)와, 혼합발효물을 습득하는 습득단계(S4)로 이루어진다.A method for producing a mixed fermented product comprising a natural product mixed medium comprises a medium preparation step (S1) for preparing a natural product mixed medium, a mushroom seed preparation step (S1 ') for preparing a mushroom seed culture to be cultivated in the natural product mixed medium, (S2) inoculating the mushroom seedlings prepared in the mushroom seedling preparation step (S1 '), a fermentation step (S3) of culturing and fermenting the natural material mixed medium inoculated with the mushroom seedlings, and a fermentation step (S4).
상기 천연물 혼합배지로 사용하기 위한 배지준비단계(S1)는, 울금을 선별-세척-마쇄단계를 거쳐서 얻은 마쇄물과, 알로에를 선별-세척- 제피 - 마쇄 단계를 거쳐서 얻은 점질상 겔을 발효배양조에 울금을 1~30중량%와 알로에를 70~99중량%로 투입하여 120~125℃로 20~50분간 가열멸균처리하고, 실온(20~25℃)으로 급랭 처리한다.The medium preparation step (S1) for use as the natural material mixed medium is characterized in that the viscous gel obtained through the step of screening-washing-grinding the seedlings and the aloe-selective-washing- The mixture is heated to 120 to 125 ° C for 20 to 50 minutes and sterilized at room temperature (20 to 25 ° C).
본 천연물 혼합배지는 다른 화학적인 첨가물질을 전혀 첨가하지 않고, 울금과 알로에로 이루어진 100% 천연물 혼합배지로 사용하게 된다.This natural substance mixed medium will be used as a 100% natural material mixed medium consisting of corn and aloe without adding any chemical additives.
상기 알로에는 생엽을 사용하며, 보통 잎을 자르면 나오는 맑은 겔(젤) 형태의 수액이 나오며, 상기 수액은 화상과 관절염을 치료하는 효과가 있는 알로에 베라(aloe vera)를 사용한다.
The aloe uses a fresh leaf, and a clear gel (gel) -type liquid which comes out when cutting leaves is usually used. The sap uses an aloe vera which has an effect of treating burns and arthritis.
상기 버섯종균을 준비하는 버섯종균준비단계(S1')는, 버섯종균을 본 발명의 발효 종균으로 사용할 수 있게 최적조건에서 배양하는 것으로, 상기 버섯종균을 온도 22~30℃, 통기량 0.3~0.8vvm, shaking rate 100~140 rpm 조건하에서 4 ~ 7일간 배양하며, 상기 버섯종균은 영지버섯(Ganoderma lucidum), 상황버섯(Phellinus linteus), 붉은 동충하초(Cordyceps militaris ), 누에 동충하초(Paecilomyces japonica), 노루궁뎅이버섯(Hericium erinaceum), 아가리쿠스 브라제(Agaricus blazei)으로 이루어진 군으로부터 1종을 선택하여 단독으로 사용한다.
The mushroom seedling preparation step (S1 ') for preparing the mushroom seedlings comprises culturing the mushroom seedlings at an optimum condition so as to be usable as the fermentation seedlings of the present invention, wherein the mushroom seedling is cultured at a temperature of 22 to 30 ° C, vancomycin, vvm,
상기 버섯종균을 접종하는 접종단계(S2)는, 배지준비단계(S1)에서 준비된 천연물 혼합배지가 들어있는 배양조에 버섯종균준비단계(S1')에서 기 배양한 버섯 전배양액(종균)을 접종하는 것으로, 상기 천연물 혼합배지에 6~10%(w/w)로 버섯종균을 접종한다.
The inoculation step (S2) of inoculating the mushroom seedlings is performed by inoculating the mushroom precursor culture (seeds) cultured in the mushroom seedling preparation step (S1 ') into a culture tank containing the natural material mixed medium prepared in the medium preparation step (S1) , And the mushroom seedlings are inoculated to the natural material mixed medium at 6 to 10% (w / w).
상기 발효단계(S3)는, 버섯 전배양액(종균)을 접종한 천연물 혼합배지를 최적조건(온도 : 22~30℃, 통기량 0.4~0.8vvm)에서 3~10일 동안 배양 및 발효시킨다.The fermentation step (S3) comprises culturing and fermenting a natural material mixed medium inoculated with a mushroom preculture (seed culture) for 3 to 10 days under optimum conditions (temperature: 22 to 30 DEG C, aeration amount: 0.4 to 0.8 vvm).
상기 천연물 혼합배지가 들어있는 배양조에 배양되는 버섯종균(전배양액)은 그 종균의 종류에 따라 발효단계(S3)의 온도 및 기간에서 약간의 차이가 있다.The mushroom seed culture (pre-culture solution) cultured in the culture tank containing the natural material mixed medium has a slight difference in the temperature and duration of the fermentation stage (S3) depending on the kind of the seed culture.
상세하게는 영지버섯(Ganoderma lucidum)의 경우 종균접종 후 최적배양온도는 26~28℃, 통기량은 0.3 ~ 0.5vvm 조건하에서 배양하는 것이 적당하며, 붉은 동초하초(코디셉스 밀리타리스;Cordyceps militaris )의 경우 종균접종 후 최적배양온도는 23~25℃, 통기량은 0.3 ~ 0.5vvm 조건 하에서 배양하는 것이 적당하며, 상황버섯(페리누스 린테우스;Phellinus linteus)의 경우 종균접종 후 최적배양온도는 28~30℃, 통기량은 0.4 ~ 0.6vvm 조건 하에서 배양하는 것이 적당하며, 노루궁뎅이버섯(헤리시움 에리나세움;Hericium erinaceum)의 경우 최적배양온도는 22~24℃, 통기량은 0.4 ~ 0.6vvm 조건 하에서 배양하는 것이 바람직하다.In detail, ganoderma ( Ganoderma lucidum ), the optimal culture temperature after inoculation is 26 ~ 28 ℃ and the aeration is 0.3 ~ 0.5 vvm. In case of Cordyceps militaris , Culturing is preferably carried out at a temperature of 23 to 25 ° C and an aeration rate of 0.3 to 0.5 vvm. The culture is carried out under conditions of a mushroom ( Phellinus linteus ), it is appropriate to cultivate under the conditions of optimal culture temperature 28 ~ 30 ℃ and aeration 0.4 ~ 0.6vvm after inoculation of seedling , and it is suitable to cultivate Hericium mushroom ( Hericium erinaceum ; erinaceum ), it is preferable to cultivate under the conditions of optimum culture temperature of 22 to 24 ° C and aeration amount of 0.4 to 0.6 vvm.
이와 같이 천연물 혼합배지에 (약용)버섯 종균을 접종할 때는 각각의 버섯종균의 특성 및 배양 정도에 따라 차이가 있으나, 보통 22~30℃의 온도와, 0.3~0.8vvm의 통기량의 조건하에서 3~10의 배양일수의 범위 내에서 조절하면서 발효배양조에 배양되는 버섯종균을 준비한다.When the mushroom seedlings are inoculated to the natural medium mixture medium, the mushroom seedlings are differentiated according to the characteristics and the degree of cultivation of each mushroom seedlings. However, under the condition of the temperature of 22 to 30 ° C and the aeration amount of 0.3 to 0.8 vvm, Prepare the mushroom seeds cultured in the fermentation incubator while adjusting within the range of ~ 10 culture days.
상기와 같은 제조방법으로 제조된 혼합발효물(울금-알로에 천연혼합발효물)을 이용하여 직접 및 간접의 방식으로 섭취할 수 있도록 식품의 원료로 제공될 수 있다.Can be provided as a raw material for foods to be ingested directly or indirectly by using the mixed fermented product (natural fermented product of Ulgum-Aloe) manufactured by the above-described production method.
상기 혼합발효물을 이용한 기능성 식품의 제조방법은,A method for producing a functional food using the mixed fermented product,
첫째, 혼합발효물을 직접섭취가 가능한 기능성 식품으로의 제조방법으로, 수득한 혼합발효물을 직접 섭취가 가능하도록 포장하여 제품화한다.First, as a method for manufacturing a functional food capable of directly ingesting a mixed fermented product, the obtained fermented product is packaged and manufactured into a product so that it can be directly ingested.
둘째, 혼합발효물을 동결건조하여 기능성 식품의 원료로 사용하는 방법으로, 수득한 혼합발효물을 간접 섭취가 가능하도록 식품의 부원료로 동결건조된 혼합발효물을 식품의 부원료로 첨가하여 기능성 식품을 제조한다.Second, the method of freeze-drying the mixed fermented product to use as a raw material of the functional food, the mixed fermented product which is freeze-dried as an auxiliary ingredient of the food is added as an additive to the food so that the indirectly ingested mixed fermented product can be used, .
셋째, 혼합발효물을 음료의 주원료로 함으로써 기능성 식품의 음료로 제조한다.Third, the mixed fermented product is made into beverage of functional food by making it the main ingredient of beverage.
즉, 상기와 같은 제조방법으로 제조된 혼합발효물은 음료형태, 캡술 형태 및 정제형태 등과 같이 다양하게 가공하여 제품화할 수 있는 것으로, 상기 혼합발효물의 추출물을 유효성분으로 하는 다양한 간 기능 개선용 제제로 사용될 수 있다.
That is, the mixed fermented product produced by the above-described production method can be processed into various products such as beverage form, capsulse form and tablet form, and it can be produced by various kinds of preparations for improving liver function .
이하, 실시예를 통해 본 발명인 간 기능 개선용 혼합발효물(울금-알로에 천연혼합발효물) 제조와 이를 이용한 기능성 식품을 상세히 설명한다.
Hereinafter, the preparation of the mixed fermented product for improving liver function (natural fermented product of Ulleung-Aloe) according to the present invention and the functional food using the same will be described in detail.
실시예Example 1. One.
울금Kool -- 알로에으로By Aloe 이루어진 천연물 Made natural product 혼합배지와Mixed media and 영지버섯( Ganoderma lucidum ( GanodermaGanoderma lucidumlucidum )을 종균() Was used as a seed ( 전배양액Pre-culture solution )으로 한 ) 혼합발효물Mixed fermentation product 제조방법, Manufacturing method,
① 천연물 혼합배지를 준비하는 단계(S1) : 먼저 알로에 생엽을 선별 - 세척 - 제피 - 마쇄 단계를 거쳐서 점질상의 겔을 준비하고, 울금도 선별-세척-마쇄하는 단계를 거쳐서 마쇄액을 얻은 다음, 알로에 겔과 울금 마쇄액을 8:2의 중량비율로 혼합하여 발효배양조에 투입한 다음 120℃에서 20분간 멸균한 다음 온도를 22℃까지 급랭하여 100% 천연물 혼합배지를 준비한다.(1) Step of preparing a natural material mixed medium (S1): First, a gel of a viscous gel is prepared through a step of screening-washing-skin-dressing the raw material of aloe, and a step of screening, washing, Aloe gel and corn starch are mixed at a weight ratio of 8: 2, put into a fermentation culture tank, sterilized at 120 ° C for 20 minutes, and then cooled to 22 ° C to prepare a 100% natural material mixed medium.
② 영지버섯 종균을 준비하는 단계(S1') : 영지버섯 균을 종균(전배양액)으로 사용하기 위해, 온도 28℃, Shaking Rate 120 RPM에서 4일간 배양하여 종균(전배양액)을 준비한다.② Preparation step (S1 '): Prepare the seed culture (pre-culture solution) for 4 days at a temperature of 28 ° C and a shaking rate of 120 RPM to use the mushroom as a seed culture.
③ 영지버섯균을 접종하는 접종단계(S2) : 상기 단계(S1)에서 준비된 천연물 혼합배지에 단계(S1')에서 준비한 영지버섯 전배양액(종균)을 8%(w/w)를 접종한다.(3) Inoculation step of inoculating Ganoderma lucidum (S2): 8% (w / w) of the Ganoderma lucidum preparation prepared in step (S1 ') is inoculated into the natural material mixed medium prepared in step (S1).
④ 영지버섯 종균을 접종한 천연물 혼합배지를 배양 및 발효시키는 발효단계(S3) : 상기 단계(S2)에서 영지버섯을 접종한 천연물 혼합배지를 최적조건(Temperature : 28℃, 통기량은 0.5vvm)에서 6일 동안 배양 발효시킨다.(3) Fermentation step (S3) of cultivating and fermenting the natural material mixed medium inoculated with the mushroom seedlings of Ganoderma lucidum: In the step (S2), the natural medium mixed medium inoculated with Ganoderma lucidum was subjected to an optimum condition (temperature: 28 ° C, aeration amount: 0.5 vvm) Lt; / RTI > for 6 days.
⑤ 마지막으로 혼합발효물을 수득하는 단계(S4) : 상기 단계(S3)에서 배양이 끝난 간 기능을 개선시킬 수 있는 혼합발효물(울금-알로에 천연혼합발효물)을 수득한다.
(5) Finally, step (S4) of obtaining a mixed fermented product: In step (S3), a mixed fermented product (natural fermented product of Ulgi-Aloe) which can improve the liver function after cultivation is obtained.
실시예Example 2. 2.
울금Kool -- 알로에으로By Aloe 이루어진 천연물 Made natural product 혼합배지와Mixed media and 상황버섯( Situ mushroom ( PhellinusPhellinus linteuslinteus )을 종균() Was used as a seed ( 전배양액Pre-culture solution )으로 한 ) 혼합발효물Mixed fermentation product 제조방법, Manufacturing method,
① 천연물 혼합배지를 준비하는 단계(S1) : 먼저 알로에 생엽을 선별 - 세척 - 제피 - 마쇄 단계를 거쳐서 점질상의 겔을 준비하고, 울금도 선별-세척-마쇄하는 단계를 거쳐서 마쇄액을 얻은 다음, 알로에 겔과 울금마쇄액을 8:2의 중량비율로 혼합하여 발효배양조에 투입한 다음 120℃에서 20분간 멸균한 다음 온도를 22℃까지 급랭하여 100% 천연물 혼합배지를 준비한다.(1) Step of preparing a natural material mixed medium (S1): First, a gel of a viscous gel is prepared through a step of screening-washing-skin-dressing the raw material of aloe, and a step of screening, washing, Aloe gel and corn starch are mixed at a weight ratio of 8: 2, put into a fermentation culture tank, sterilized at 120 ° C for 20 minutes, and then cooled to 22 ° C to prepare a 100% natural material mixed medium.
② 상황버섯 종균을 준비하는 단계(S1') : 상황버섯 균을 종균(전배양액)으로 사용하기 위해, 온도 28℃, Shaking Rate 120 RPM에서 5일간 배양하여 종균(전배양액)을 준비한다.② Preparation phase (S1 '): Prepare the seed culture (pre-culture solution) for 5 days at a temperature of 28 ° C and a shaking rate of 120 RPM in order to use the mushroom as a seed culture (pre-culture solution).
③ 상황버섯을 접종하는 접종단계(S2) : 상기 단계(S1)에서 준비된 천연물 혼합배지에 단계(S1')에서 준비한 상황버섯 전배양액(종균)을 7%(w/w)를 접종한다.(3) Inoculation step (S2): Inoculate 7% (w / w) of the mushroom preculture liquid prepared in step (S1 ') into the natural material mixed medium prepared in step (S1).
④ 상황버섯 종균을 접종한 천연물 혼합배지를 배양 및 발효시키는 발효단계(S3) : 상기 단계(S2)에서 상황버섯을 접종한 천연물 혼합배지를 최적조건(Temperature : 28℃, 통기량은 0.5vvm)에서 7일 동안 배양 발효시킨다.(4) Fermentation step (S3) of cultivating and fermenting the natural material mixed medium inoculated with the mushroom seedlings: In the step (S2), the natural material mixed medium inoculated with the mushroom was subjected to an optimum condition (temperature: 28 ° C, aeration amount: 0.5 vvm) Lt; / RTI > for 7 days.
⑤ 마지막으로 혼합발효물을 수득하는 단계(S4) : 상기 단계(S3)에서 배양이 끝난 간 기능을 개선시킬 수 있는 혼합발효물(울금-알로에 천연혼합발효물)을 수득한다.(5) Finally, step (S4) of obtaining a mixed fermented product: In step (S3), a mixed fermented product (natural fermented product of Ulgi-Aloe) which can improve the liver function after cultivation is obtained.
실시예Example 3. 3.
울금Kool -- 알로에으로By Aloe 이루어진 천연물 Made natural product 혼합배지와Mixed media and 붉은( Red( 밀리타리스Millitaris )동충하초() Cordyceps ( Cordyceps militarisCordyceps militaris )을)of 종균( Seed bacteria ( 전배양액Pre-culture solution )으로 한 ) 천연물혼합발효물Natural fermented product 제조방법, Manufacturing method,
① 천연물 혼합배지를 준비하는 단계(S1) : 먼저 알로에 생엽을 선별 - 세척 - 제피 - 마쇄 단계를 거쳐서 점질상의 겔을 준비하고, 울금도 선별-세척-마쇄하는 단계를 거쳐서 마쇄액을 얻은 다음, 알로에 겔과 울금마쇄액을 8:2의 중량비율로 혼합하여 발효배양조에 투입한 다음 120℃에서 20분간 멸균한 다음 온도를 22℃까지 급랭하여 100% 천연물 혼합배지를 준비한다. (1) Step of preparing a natural material mixed medium (S1): First, a gel of a viscous gel is prepared through a step of screening-washing-skin-dressing the raw material of aloe, and a step of screening, washing, Aloe gel and corn starch are mixed at a weight ratio of 8: 2, put into a fermentation culture tank, sterilized at 120 ° C for 20 minutes, and then cooled to 22 ° C to prepare a 100% natural material mixed medium.
② 붉은(밀리타리스)동충하초 종균을 준비하는 단계(S1') : 붉은(밀리타리스)동충하초 균을 종균(전배양액)으로 사용하기 위해, 온도 24℃, Shaking Rate 120 RPM에서 6일간 배양하여 종균(전배양액)을 준비한다.② Preparation stage of red (Millitaris) Cordyceps spp. (S1 '): In order to use Red (Millitaris) Cordyceps spp. As a seed culture (pre-culture solution), it was cultured at 24 ℃ and Shaking
③ 붉은(밀리타리스)동충하초을 접종하는 접종단계(S2) : 상기 단계(S1)에서 준비된 천연물 혼합배지에 단계(S1')에서 준비한 붉은(밀리타리스)동충하초 전배양액(종균)을 8%(w/w)를 접종한다.3) Inoculation step (S2) of inoculating red (Millitaris) Cordyceps: The red (Millitaris) pre-culture solution prepared in step S1 'is added to the natural material mixed medium prepared in step S1 in an amount of 8% w / w).
④ 붉은(밀리타리스)동충하초 종균을 접종한 천연물 혼합배지를 배양 및 발효시키는 발효단계(S4) : 상기 단계(S2)에서 붉은(밀리타리스)동충하초을 접종한 천연물 혼합배지를 최적조건(Temperature : 24℃, 통기량은 0.5vvm)에서 6일 동안 배양 발효시킨다. (4) Fermentation step (S4) of cultivating and fermenting a natural material mixed medium inoculated with red (Millitaredis) seedlings: In the step (S2), a natural material mixed medium inoculated with red (Millitaris) 24 ° C, aeration volume 0.5 vvm) for 6 days.
⑤ 마지막으로 혼합발효물을 수득하는 단계(S4) : 상기 단계(S3)에서 배양이 끝난 간 기능을 개선시킬 수 있는 혼합발효물(울금-알로에 천연혼합발효물)을 수득한다.
(5) Finally, step (S4) of obtaining a mixed fermented product: In step (S3), a mixed fermented product (natural fermented product of Ulgi-Aloe) which can improve the liver function after cultivation is obtained.
실시예Example 4 4
울금-알로에으로 이루어진 천연물 혼합배지와 노루궁뎅이버섯(Hericeum erinaceum)을 종균(전배양액)으로 한 천연물혼합발효물 제조방법,A method for producing a fermented natural product mixture comprising a mixture of a natural material consisting of ganoderma lucidum and aloe, and Hericeum erinaceum as a seed culture,
① 천연물 혼합배지를 준비하는 단계(S1) : 먼저 알로에 생엽을 선별 - 세척 - 제피 - 마쇄 단계를 거쳐서 점질상의 겔을 준비하고, 울금도 선별-세척-마쇄하는 단계를 거쳐서 마쇄액을 얻은 다음, 알로에 겔과 울금마쇄액을 8:2의 중량비율로 혼합하여 발효배양조에 투입한 다음 120℃에서 20분간 멸균한 다음 온도를 22℃까지 급랭하여 100% 천연물 혼합배지를 준비한다. (1) Step of preparing a natural material mixed medium (S1): First, a gel of a viscous gel is prepared through a step of screening-washing-skin-dressing the raw material of aloe, and a step of screening, washing, Aloe gel and corn starch are mixed at a weight ratio of 8: 2, put into a fermentation culture tank, sterilized at 120 ° C for 20 minutes, and then cooled to 22 ° C to prepare a 100% natural material mixed medium.
② 노루궁뎅이버섯 종균을 준비하는 단계(S1') : 노루궁뎅이버섯 균을 종균(전배양액)으로 사용하기 위해, 온도 24℃, Shaking Rate 120 RPM에서 5일간 배양하여 종균(전배양액)을 준비한다.② Step (S1 ') for preparation of seedling mushroom seedlings: For use as seedling mushroom strain (pre-culture solution), seed culture (pre-culture solution) is prepared by culturing at 24 ° C. and shaking
③ 노루궁뎅이버섯을 접종하는 접종단계(S2) : 상기 단계(S1)에서 준비된 천연물 혼합배지에 단계(S1')에서 준비한 노루궁뎅이버섯 전배양액(종균)을 7%(w/w)를 접종한다. (3) Inoculation step (S2): Inoculation of 7% (w / w) pre-culture medium (seedling) prepared in step (S1 ') in the natural material mixed medium prepared in step (S1) .
④ 노루궁뎅이버섯 종균을 접종한 천연물 혼합배지를 배양 및 발효시키는 단계(S3) : 상기 단계(S2)에서 노루궁뎅이버섯을 접종한 천연물 혼합배지를 최적조건(Temperature : 24℃, 통기량은 0.5vvm)에서 6일 동안 배양 발효시킨다. (4) Culturing and fermenting the natural material mixed medium inoculated with the seedling mushroom seedlings (S3): In the step (S2), the natural material mixed medium inoculated with the seedling mushroom was subjected to an optimum condition (temperature: 24 캜, aeration amount of 0.5 vvm ) For 6 days.
⑤ 마지막으로 혼합발효물을 수득하는 단계(S4) : 상기 단계(S3)에서 배양이 끝난 간 기능을 개선시킬 수 있는 혼합발효물(울금-알로에 천연혼합발효물)을 수득한다.
(5) Finally, step (S4) of obtaining a mixed fermented product: In step (S3), a mixed fermented product (natural fermented product of Ulgi-Aloe) which can improve the liver function after cultivation is obtained.
[비교 실험][Comparative experiment]
1. One. 혼합발효물의Mixed fermented ADHADH 의 활성측정Activity measurement
① 혼합발효물(실시예1 ~ 실시예4)을 추출한 다음 여과 및 농축하여 동결건조기를 이용하여 sample화하여 사용한다. (1) Extraction of mixed fermented products (Examples 1 to 4), followed by filtration and concentration, followed by sampling using a freeze dryer.
효소준비 : 효소원은 동결 건조된 S9 rat liver homogenate(MOLTOX Co., USA)를 0.1% bovine serum albumin 용액 8ml에 녹여 0.45mcm syringe filter로 여과하여 사용. Enzyme preparation: Lyophilized S9 rat liver homogenate (MOLTOX Co., USA) was dissolved in 8 ml of 0.1% bovine serum albumin solution and filtered with 0.45mcm syringe filter.
ADH활성의 측정 : Blandino가 사용한 방법을 변형한 것으로 흡광도 340nm에서 NADH의 생성속도를 지표로 사용하였다. 반응액의 조성은 증류수가 1.4ml, 1.0 M tris-HCl buffer(pH 8.8) 0.75ml, 20 mM NAD+ 0.3ml, ethanol 0.3ml, Sample 0.1ml의 혼합액과 효소원 0.15ml를 cuvette에 넣어 총 3ml가 되게 조절하여 30℃에서 5분간 pre-incubation 한 후, 5분간 340nm에서 흡광도의 변화를 측정하였다. 이때 시료를 첨가하지 않은 것을 대조군으로 하였다. Measurement of ADH activity: A modification of the method used by Blandino, the rate of NADH formation at 340 nm absorbance was used as an indicator. The reaction mixture was prepared by adding 1.4 ml of distilled water, 0.75 ml of 1.0 M tris-HCl buffer (pH 8.8), 0.3 ml of 20 mM NAD +, 0.3 ml of ethanol and 0.1 ml of sample and 0.15 ml of enzyme source into a cuvette. After pre-incubation at 30 ° C for 5 minutes, the absorbance was measured at 340 nm for 5 minutes. At this time, a sample to which no sample was added was used as a control.
시료의 ADH활성은 control에 대한 상대활성(%)으로 측정하였다.The ADH activity of the sample was measured as the relative activity (%) relative to the control.
결과 : 100ppm 농도에서 실시예 1인 천연물 혼합배지-영지버섯의 혼합발효물과, 실시예 2인 천연물혼합배지-상황버섯의 혼합발효물과, 실시예 3인 천연물 혼합배지-동충하초의 혼합발효물과, 실시예 4인 천연물 혼합배지-노루궁뎅이버섯의 혼합발효물의 ADH activity 이 각각 115%( 실시예 1), 109%( 실시예 2), 114%( 실시예 3) 및 121%(실시예 4)로 나타내었으며, 숙취해소음료의 소재로 잘 알려진 구르메(Gurume)를 같은 방법으로 실험하여 ADH 의 활성이 108%로 나타났음.
Results: The mixed fermented product of natural product mixed medium-gonorrhea mushroom of Example 1, mixed product of natural product mixed medium and mushroom of Example 2, mixed product of natural product of fermented product of Example 3 and mixed product of cucumber And the mixed product of the natural product of Example 4 and the ADH activity was shown by 115% ( Example 1), 109% ( Example 2), 114% ( Example 3) and 121% (Example 4 ) And the activity of ADH was 108% .
2. 2. 혼합발효물의Mixed fermented ALDHALDH 의 활성측정Activity measurement
① 혼합발효물(실시예1 ~ 실시예4)을 추출한 다음 여과 및 농축하여 동결건조기를 이용하여 sample화하여 사용한다.(1) Extraction of mixed fermented products (Examples 1 to 4), followed by filtration and concentration, followed by sampling using a freeze dryer.
효소준비 : 효소원은 동결 건조된 S9 rat liver homogenate(MOLTOX Co., USA)를 0.1% bovine serum albumin 용액 8ml에 녹여 0.45mcm syringe filter로 여과하여 사용.Enzyme preparation: Lyophilized S9 rat liver homogenate (MOLTOX Co., USA) was dissolved in 8 ml of 0.1% bovine serum albumin solution and filtered with 0.45mcm syringe filter.
ALDH의 활성측정 : Bostian의 방법을 변형하여 측정하는 것으로, 흡광도 340nm에서 ADH 생성속도를 지표로 사용하였다. 반응액의 조성은 증류수 2.1ml, 1.0M tris-HCl buffer(pH 8.0) 0.3ml, 20mM NAD+ 0.1ml, 1.0 M acetaldehyde 0.1ml, 3.0M KCl 0.1ml, 0.33 M 2-mercaptoethanol 0.1ml, Sample 0.1ml의 혼합액과 효소원 0.1ml를 cuvette에 넣어 총 3ml가 되게 조절하여 30℃에서 5분간 pre-incubation 한 후, 5분간 340nm에서 흡광도의 변화를 측정하였다. 이때 시료를 첨가하지 않은 것을 대조군으로 하였다. 시료의 ALDH활성은 control에 대한 상대활성(%)으로서 측정하였다.Measurement of the activity of ALDH: It was measured by modifying the Bostian method. The ADH production rate was used as an index at an absorbance of 340 nm. The composition of the reaction solution was 2.1 ml of distilled water, 0.3 ml of 1.0 M tris-HCl buffer (pH 8.0), 0.1 ml of 20 mM NAD + 0.1 ml of 1.0 M acetaldehyde, 0.1 ml of 3.0 M KCl, 0.1 ml of 0.33 M 2-mercaptoethanol, And 0.1 ml of the enzyme source were added to the cuvette to adjust the total volume to 3 ml. After preincubation at 30 ° C for 5 minutes, the absorbance was measured at 340 nm for 5 minutes. At this time, a sample to which no sample was added was used as a control. The ALDH activity of the sample was measured as a relative activity (%) relative to the control.
결과 : 100ppm 농도에서 실시예 1인 천연물 혼합배지-영지버섯의 혼합발효물과, 실시예 2인 천연물혼합배지-상황버섯의 혼합발효물과, 실시예 3인 천연물 혼합배지-동충하초의 혼합발효물과, 실시예 4인 천연물 혼합배지-노루궁뎅이버섯의 혼합발효물의 ALDH activity 이 각각 96%, 94%, 92% 및 98%로 나타내었으며, 현재 숙취해소음료의 소재로 잘 알려진 구르메(Gurume)를 같은 방법으로 실험하여 ALDH 의 활성이 90%로 나타났음.
Results: The mixed fermented product of natural product mixed medium-gonorrhea mushroom of Example 1, mixed product of natural product mixed medium and mushroom of Example 2, mixed product of natural product of fermented product of Example 3 and mixed product of cucumber And the mixed fermented product of the natural product mixed medium of Example 4, Herring mushroom, ALDH activity was 96%, 94%, 92%, and 98% , respectively. The activity of ALDH was shown to be 90% by the same method as Gurume which is well known as hangover drink.
3. 3. 혼합발효물의Mixed fermented 관능평가 Sensory evaluation
알로에와 울금을 혼합한 천연물 혼합물과, 천연물 혼합배지로하여 버섯종균으로 발효한 혼합발효물(실시예 1~실시예 4)의 추출물에 대한 관능평가로, 참여한 패널은 식품연구개발에 3년 이상 종사하고 있는 연구원 6인이 5점 만점으로 평가하였다.The sensory evaluation of the extracts of the natural fermented products (Examples 1 to 4) fermented with mushroom seed bacterium using natural product mixtures mixed with aloe and cucumber, and natural product mixed medium, Six researchers who were engaged in the research were rated as 5 out of 5.
상기에서와 같이 알로에와 울금로 이루어진 천연물 혼합배지에 버섯종균을 접종한 후 발효시켜 획득되는 혼합발효물(실시예 1~실시예 4)은 종래에 숙취해소음료의 소재로 잘 알려진 구르메(Gurume)에 보다 ADH와 ALDH의 활성이 높았으며, 알로에와 울금로 이루어진 천연물 혼합배지에 버섯종균을 접종한 후 발효시켜 획득되는 혼합발효물의 추출물은 알로에와 울금을 혼합한 천연물 혼합물의 추출물보다 관능미 평가(검사)에서 월등히 좋음을 알 수 있다.The mixed fermented product obtained by fermenting the mushroom seedlings after inoculating the mushroom seedlings into a natural material mixed medium consisting of aloe and cucumber as described above (Examples 1 to 4) The extracts of the mixed fermented product obtained by fermenting the mushroom seedlings after inoculation of the mushroom seeds in the mixed natural media of aloe and uroguan were higher than the extracts of the mixture of natural products mixed with aloe and cucumber, ).
그러므로 혼합발효물의 추출물은 간기능을 개선시킬 수 있는 효능과, 섭취가 용이한 동시에 음식, 음료의 보조첨가물로 용이하기 사용될 수 있어 아주 널리 사용될 수 있을 것이다.Therefore, the extract of the mixed fermentation product can be widely used because it can improve the liver function and can be easily used as an auxiliary additive for food and beverage while being easy to ingest.
Claims (7)
상기 천연물 혼합배지는 알로에 70~99중량%와 울금 1~30중량%의 비율로 혼합하고 이를 멸균처리한 혼합물로 이루어짐을 특징으로 하는 알로에와 울금을 이용한 간 기능 개선용 혼합발효물.The method according to claim 1,
Wherein the natural material mixed medium is a mixture of 70 to 99% by weight of aloe and 1 to 30% by weight of Ulgus, and the mixture is sterilized.
상기 배지준비단계(S1)에서 준비된 천연물 혼합배지에 버섯종균을 접종하는 접종단계(S2)와;
상기 접종단계(S2)에서 버섯종균을 접종한 천연물 혼합배지를 온도 22~30℃에서 4~10일 동안 배양 및 발효시키는 발효단계(S3)
를 포함하는 것을 특징으로 하는 알로에와 울금을 이용한 간 기능 개선용 혼합발효물의 제조방법.(S1) for preparing a natural material mixed medium by mixing aloe and corn using raw leaves, heating and sterilizing the mixture, and quenching;
A seeding step (S2) of inoculating the mushroom seedlings into the natural material mixed medium prepared in the medium preparation step (S1);
A fermentation step (S3) of cultivating and fermenting the natural material mixed medium inoculated with mushroom seeds at 22 to 30 ° C for 4 to 10 days in the seeding step (S2)
Wherein the aloe fermentation is carried out in the presence of an aloe.
상기 배지준비단계(S1)에서 천연물 혼합배지는 알로에 70~99중량%와, 울금 1~30 중량%으로 혼합하여 발효 배양조에 투입하여 113~125℃로 10~40분간 가열하여 멸균처리하고, 20~25℃로 급랭 처리하여 이루어짐을 특징으로 하는 알로에와 울금을 이용한 간 기능 개선용 혼합발효물의 제조방법.5. The method of claim 4,
In the medium preparation step (S1), the natural material mixture medium is mixed with 70 to 99% by weight of aloe and 1 to 30% by weight of corn, and the mixture is put into a fermentation culture tank and sterilized by heating at 113 to 125 ° C for 10 to 40 minutes, To 25 < [deg.] ≫ C. The method for producing a mixed fermented product for improving liver function using aloe and cucumber.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20220139082A (en) | 2021-04-07 | 2022-10-14 | 주식회사 휴럼 | Curcumae Radix Extract Powder Material for Liver Function Improvement Using Curcumae Radix and Lava Sea Water, HEPATIC FUNCTION-IMPROVING COMPOSITION USING THE SAME |
| KR102603876B1 (en) * | 2022-08-03 | 2023-11-20 | 농업회사법인 권도영알로에 주식회사 | Preparing method of a chicken feed composition, a chicken feed composition prepared therefrom and a method for raising chickens |
-
2012
- 2012-12-27 KR KR1020120154000A patent/KR20140084502A/en not_active Application Discontinuation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20220139082A (en) | 2021-04-07 | 2022-10-14 | 주식회사 휴럼 | Curcumae Radix Extract Powder Material for Liver Function Improvement Using Curcumae Radix and Lava Sea Water, HEPATIC FUNCTION-IMPROVING COMPOSITION USING THE SAME |
| KR102603876B1 (en) * | 2022-08-03 | 2023-11-20 | 농업회사법인 권도영알로에 주식회사 | Preparing method of a chicken feed composition, a chicken feed composition prepared therefrom and a method for raising chickens |
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