KR20140073835A - Cosmetic composition comprising of Salicornia herbacea extracts for inhibiting secretion of sebum - Google Patents

Abstract

The present invention relates to a novel use of an extract of Salicornia herbacea and a fraction thereof to inhibit sebum secretion, and specifically, to a cosmetic composition for inhibiting sebum secretion containing an extract of Salicornia herbacea and a fraction thereof as an active ingredient. The extract of Salicornia herbacea or the fraction thereof of the present invention has an antioxidant activity through active oxygen species scavenging potencies; and has functionality to control excessive sebum through effects of inhibiting triglyceride production and fat accumulation in sebum cells, so that the composition of the present invention containing the extract of Salicornia herbacea and the fraction thereof as an active ingredient has excellent effects of inhibiting inflammation and sebum secretion, and thus can be useful as a composition for functional cosmetics. Particularly, the extract of Salicornia herbacea and the fraction isolated therefrom are materials derived from a natural product and have safety without cytotoxicity, and thus the composition of the present invention containing the same as an active ingredient can be safe without skin irritation even after a long-term usage.

Description

[0001] The present invention relates to a cosmetic composition for inhibiting the production of sebum comprising Salicornia herbacea extract as an active ingredient,

The present invention relates to a novel use of the green tea extract and its fractions for inhibiting sebum production. More particularly, the present invention relates to a cosmetic composition for inhibiting sebum production comprising a green tea extract and a fraction thereof as an active ingredient.

The skin consists largely of three layers of epidermis, dermis, and subcutaneous tissue, and it is composed of hair follicles, hair follicles, sebaceous glands, etc., and is responsible for chemical, physical and biological barrier functions. The double sebaceous gland is one of the skin appendages. It is called the follicular gland and the branch line. It is formed by differentiation of the epidermis at the time of birth. It is located in the dermal layer and discharges the lipid through the pore.

If the function of the sebaceous gland is activated more than necessary, it becomes oily skin with a large amount of sebum secretion. If the excess sebum is not discharged normally, it develops into acne or causes various skin diseases such as seborrheic dermatitis due to excess sebum. The oily skin is always shiny, the pores are wide and the stratum corneum is thick, so it looks as if the skin is thick, but it does not easily develop wrinkles due to ultraviolet rays and dryness, and does not easily aging.

Acne is a chronic inflammatory disease of the skin adnexa, especially folliculitis. It is also called acne. It is a very common skin disease. It usually starts to appear in puberty and usually disappears in the mid 20s. Men are most frequently encountered between the ages of 16-19 years and women between 14-16 years of age, and severe forms of acne are known to occur more frequently in men. Clinically, it usually appears on face, neck, and chest, which are areas of sebaceous glands, which are cotton, pustules, cysts, nodules and sometimes scarring.

The exact cause of acne is not known, but it is known that various symptoms are involved and clinical symptoms are manifested by the interaction of various factors. These pathogenetic factors include 1) abnormally increased hair follicle keratinization and abnormal elimination, 2) increased secretion of sebum induced by androgens, 3) proliferation of propionibacterium acne , the causative agent of acne , 4 ) Inflammation, 5) inheritance, and 6) hair follicle reactivity.

In particular, methods for improving acne by suppressing an increase in the production of sebum (boring) are currently being studied variously. Salicylic acid, triclosan, and trihydroxy palmitic acid are used as cosmetic (Korean Patent Laid-Open Publication No. 2004-0089072) discloses a method for preventing and treating acne by opening pores and promoting secretion of sebum by applying the composition to a composition, thereby reducing sebum secretion amount in sebaceous glands by taking oligosaccharide, (Japanese Patent Application Laid-Open No. 05-294836).

On the other hand, green tea grows around the coast, mudflats and salt springs of the waters of the islands such as the west coast of Korea, the south coast, Jeju Island, Ulleungdo and Baekryeong Island. In our words, it is called "Tungtung Mardi" because it is a grass that comes out from the mouth. The old medicine book of Chinese medicine, Shingon Sangbong Sung said that the taste was very squeezed, and it was called 草 and 草, and it was regarded as a very rare and spiritual grass. It has been known since about 3,000 years ago in China that the health benefits of green tea are excellent. There is a record in the circumcision that the King of kings gave sacrifices to the heaven in the sky. It is said that the green tea is a precious longevity which makes longevity live by fire in Kaibara 's Daisai Hokusa, which is called "the medicine of the Japanese". There are various names such as. The common components of green tea are mainly carbohydrates, ash, crude protein and crude fat, and salt accounts for 13.5% by weight in ash. In addition, it contains various minerals in green tea. It is known that 100 g of green tea contains 670 mg of calcium, 70 mg of iodine, 6.5% of sodium, 16% of salt, and 50% of vegetable fiber.

Accordingly, the present inventors have searched for a material derived from a natural substance that is stable to human body and exhibits an excellent effect of suppressing sebum production, while the antioxidant activity and antioxidative activity of the Hamchon extract and the functional fraction isolated therefrom by high- The present inventors have completed the present invention by confirming that the production of sebum can be effectively inhibited.

Korean Patent Publication No. 10-2004-0089072

Accordingly, an object of the present invention is to provide a cosmetic composition which is excellent in activity to regulate excessive sebum secretion in sebaceous cells and is stable to human skin, and is effective in inhibiting sebum production.

In order to accomplish the object of the present invention, the present invention provides a cosmetic composition for inhibiting sebum production, comprising a green tea extract or a fraction thereof as an active ingredient.

In one embodiment of the present invention, the green tea extract may be prepared by extracting a dried green tea powder with a solvent of ethanol (ethanol); Filtering the extracted filtrate, and then concentrating the filtrate by distillation under reduced pressure; And lyophilizing and pulverizing the concentrated filtrate.

In one embodiment of the present invention, the fraction is obtained by extracting a dried green tea powder with a solvent of ethanol (ethanol); Filtering the extracted filtrate, and then concentrating the filtrate by distillation under reduced pressure; Performing high-speed chromatography on the concentrate to separate the functional fractions; And lyophilizing the separated fractions and pulverizing them.

In one embodiment of the present invention, the green tea extract or its fraction may be contained in an amount of 0.001 to 10% by weight based on the dry weight of the composition.

In one embodiment of the present invention, the green tea extract or fraction thereof has antioxidant activity; And the ability to regulate excess sebum through inhibition of triglyceride formation and inhibition of fat accumulation in sebaceous cells.

In one embodiment of the present invention, the green tea extract or its fractions may inhibit fat production in sebum cells through a mechanism of reducing the expression of SREBP-1 PPAR?, PPAR ?, C / EBP? .

In one embodiment of the present invention, the composition may be a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritive cream, And may be formulated into one selected from the group consisting of a pack, a soap, a shampoo, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, a latex, a lipstick, a makeup base, a foundation, a press powder and a loose powder .

The green tea extract or its fractions of the present invention have antioxidant activity through active oxygen species scavenging ability; And the ability to control hyperglycemia through inhibition of triglyceride formation and lipid accumulation in sebaceous cells. Therefore, the composition of the present invention containing it as an active ingredient is excellent in the effect of inhibiting inflammation and sebum formation, . ≪ / RTI > In particular, the green tea extract or the fraction isolated therefrom of the present invention is a substance derived from a natural product and has stability without cytotoxicity, and therefore, the composition of the present invention containing it as an effective ingredient has a safety advantage over long-term use without irritation to the skin .

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view showing a manufacturing process of a green tea leaf extract of the present invention.
FIG. 2A is a graph showing the cell survival rate measured 48 hours after treating the human sebaceous gland cells with the green tea extract of the present invention by concentration.
FIG. 2B is a graph showing the cell survival rate at 24 hours and 48 hours after treating the human sebaceous cell with the linoleic acid and the concentration of the green tea extract of the present invention.
FIG. 3 is a graph showing the activity of scavenging the active oxygen species according to the concentration of the green tea extract of the present invention.
FIG. 4 is a graph showing the degree of suppression of triglyceride production according to the concentration of the green tea extract of the present invention.
5 is a photograph showing adipose cells accumulated in sebum cells after treatment with human leishmania extract (100 μg / ml) of the present invention together with linoleic acid (100 μg / ml) to human sebum cells.
FIG. 6 shows the results of Western blot analysis of the amount of protein expressed in the cells, SREBP-1 PPARα, PPARγ, and C / EBPβ, after treating the human sebaceous cell with linoleic acid (100 μg / ml) It is.
FIG. 7 is a data graph obtained by experimentally examining the suppression effect of sebum secretion of the green tea extract of the present invention.

The present invention relates to a new use for inhibiting sebum production of a green tea extract or a fraction thereof, and is characterized by providing a sebum production inhibiting cosmetic composition comprising a green tea extract or a fraction thereof as an active ingredient.

The term " Salicornia & herbacea 'is an annual herbaceous herbaceous plant of the dicotyledonous plant, and grows around the coast, mudflats and salt marshes that reach the waters of the islands such as the west coast of Korea, the south coast, Jeju Island, Ulleungdo and Baekryeong Island. In our words, it is called "Tungtung Mardi" because it is a grass that comes out from the mouth. The stem has many nodes, prominent, split 1-2 times, the branch is larval, fleshy, hypertrophic, dark green, later red, no leaf. The flowers are green, ears, lanceolate, hanging on the end of branches, and 2-3 small flowers hiding in hollows facing each other. The horns are inverted cones, fleshy, plump, and grow after the flowers and surround the fruit. Surgery is 1-2, ovary is ovate, style is 2 long. Fruits are covered with feces, flat ovate,

The inventors of the present invention found that the extracts of the green tea extract and the fractions isolated therefrom exhibit the antioxidant activity and the functionality capable of controlling the excess sebum by inhibiting the formation of triglycerides and inhibiting fat accumulation in sebaceous cells , Thereby effectively inhibiting the production of sebum in the cells.

As a result of examining the antioxidative activity of the extract of the present invention through the DCFH-DA essence, it was found that in the test group treated with the extract of the present invention, H ₂ O ₂ (See Fig. 3).

In another embodiment of the present invention, the sebum production inhibitory activity of the extract of the present invention was examined. In the test group treated with linoleic acid and the extract of the present invention, (See FIG. 4), inhibiting the accumulation of fat in the cells (see FIG. 5), and confirming that protein expression of SREBP-1 PPARα, PPARγ, and C / EBPβ, which are fat production-related factors, (See FIG. 6).

In another embodiment of the present invention, in order to clinically confirm whether or not the extract of the present invention has the effect of improving sebum actually, each of 10 male and 20 female subjects aged 20-30 who are active in sebum secretion, The extracts (100 μg / ml) were applied to the skin, and after 1 week and 2 weeks, the degree of decrease in oiliness was measured at the brow and nodule. As a result, (See Fig. 7).

From these results, the present inventors have experimentally proved that the green tea extract and its fractions simultaneously have antioxidative activity and excessive sebum control function.

Therefore, the composition of the present invention containing the green tea extract and the fraction thereof as an active ingredient can effectively inhibit sebum production.

The extract of the present invention can be obtained by extracting and isolating from nature using an extraction and separation method known in the art, and the 'extract' defined in the present invention can be obtained by extracting from a green tea For example, crude extracts of green tea, polar solvent-soluble extracts, or non-polar solvent-soluble extracts. Preferably a non-polar solvent-soluble extract of dried green tea.

Examples of suitable solvents for extracting the extract from green tea include any pharmaceutically acceptable organic solvent, and water or an organic solvent may be used. Examples of the solvent include, but are not limited to, purified water, methanol acetone, ether, benzene, chloroform (such as methanol, ethanol, propanol, isopropanol, and butanol) chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane may be used alone or in combination.

In one embodiment of the present invention, when the green tea extract of the present invention is a non-polar solvent-soluble extract, it can be extracted using ethanol (alcohol).

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the method for producing the green tea extract of the present invention, and any known method can be used.

For example, the green tea extract contained in the composition of the present invention can be prepared into a powdery state by an additional process such as vacuum distillation, freeze-drying, spray drying, or the like, with the primary extract extracted by the hot water extraction or solvent extraction method described above . Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, in the present invention, the leek extract is a concept including all the extracts, fractions and tablets obtained in each step of extraction, fractionation or purification, their diluted solutions, concentrates or dried products.

The method for producing the green tea extract according to one embodiment of the present invention will be described in more detail as follows.

First, the green tea extract of the present invention is prepared by extracting a dried green tea powder with a solvent of ethanol (ethanol); Filtering the extracted filtrate, and then concentrating the filtrate by distillation under reduced pressure; And lyophilizing and pulverizing the concentrated filtrate.

The fraction isolated from the green tea extract of the present invention may be obtained by extracting a dried green tea powder with a solvent of ethanol (ethanol); Filtering the extracted filtrate, and then concentrating the filtrate by distillation under reduced pressure; Performing high-speed chromatography on the concentrate to separate the functional fractions; And lyophilizing the separated fractions and pulverizing them.

When the composition of the present invention is prepared with a cosmetic composition, the composition of the present invention may contain not only the above-mentioned green tea extract or its fractions, but also components commonly used in cosmetic compositions such as antioxidants, stabilizers, , Customary adjuvants such as vitamins, pigments and flavoring agents, and carriers.

In addition, the composition of the present invention may be mixed with a functional ingredient having a sebum branch inhibiting application, which has been conventionally used as long as the skin protecting effect is not impaired, in addition to the above-described green tea extract or its fractions.

Examples of products to which the cosmetic composition of the present invention can be added include cosmetics such as astringent lotion, softening longevity lotion, nutrition lotion, various creams, essences, packs, foundation and the like, cleansing, cleanser, soap, .

The cosmetic composition of the present invention can be prepared into any of the formulations manufactured in the art and can be formulated into a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant- However, it is not limited thereto. As a specific formulation of the cosmetic composition of the present invention, there may be mentioned a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, It includes formulations such as soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, lipstick, makeup base, foundation, press powder, loose powder, eye shadow.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycerol aliphatic esters, polyethylene glycols or fatty acid esters of sorbitan.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane, < / RTI > butane or dimethyl ether.

When the formulation of the present invention is a suspension, a carrier such as water, ethanol, or a liquid diluent such as propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing kling, the carrier component is selected from aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, sarcosinates, fatty acids Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

In one embodiment of the present invention, the content of the green tea extract or the fraction thereof of the present invention is 0.0001-20% by weight, preferably 0.5-20%, more preferably 1.0-20% to be. If the content of the green tea extract or the fraction thereof is less than 0.0001% by weight, the effect of inhibiting the sebum production by the green tea extract or the fraction thereof is greatly reduced. If the content is more than 20% by weight, skin irritation may be caused. Lt; / RTI >

Further, in another embodiment of the present invention, the green tea extract of the present invention or a fraction thereof may be contained in the composition at a concentration of 10 to 300 μM, preferably 100 μM.

Meanwhile, the cosmetic composition according to the present invention may be formulated by incorporating a green tea extract or a fraction thereof into a nanoliposome to stabilize it. When the above-described green tea extract or its fraction is contained in the inside of the nanoliposome, the components of the green tea extract or its fractions can be stabilized to solve problems such as precipitation formation, discoloration, and deterioration during formulation and increase the solubility and transdermal absorption So that the efficacy expected from the compound can be maximally expressed.

In the present invention, nanoliposome refers to a liposome having a typical liposome form and having an average particle diameter of 10 to 500 nm. According to a preferred embodiment of the present invention, the average particle diameter of the nanoliposome is 50 to 300 nm. When the average particle diameter of the nanoliposome is more than 300 nm, improvement of skin penetration and improvement of formulation stability is very weak among technological effects to be achieved in the present invention.

The nanoliposomes used to stabilize the leek extract or its fractions according to the present invention may be prepared by a mixture comprising a polyol, an oily component, a surfactant, a phospholipid, a fatty acid and water.

The polyol used in the nanoliposome of the present invention is not particularly limited and is preferably a polyol such as propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isopropylene glycol, pentylene glycol, erythritol, , Sorbitol, and mixtures thereof. The amount thereof is 10 to 80% by weight, preferably 30 to 70% by weight, based on the total weight of the nanoliposome.

The oil component used in the preparation of the nanoliposome of the present invention may be selected from a variety of oils known in the art and is preferably a hydrocarbon oil such as hexadecane and paraffin oil, Vegetable oils such as silicon oil such as dimethicone and cyclomethicone, sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil, ethoxylated alkyl ether oils, propoxylated alkyl ether oils ₄₀ is a fatty alcohol, and mixtures thereof -, phytosphingosine, sphingosine, and scan non-ping the same scan pinggo cannabinoid lipid, cholesterol side-by celebrity, sitosterol cholesteryl sulfate, cholesteryl sulfate, cytokines, C _10. The amount thereof may be 1.0 to 30.0% by weight, and preferably 3.0 to 20.0% by weight based on the total weight of the nanoliposome.

Any surfactant known in the art may be used as the surfactant used in the preparation of the nanoliposome of the present invention. For example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants can be used. Preferred are anionic surfactants and nonionic surfactants. Specific examples of anionic surfactants include alkyl acyl glutamates, alkyl phosphates, alkyl lactylates, dialkyl phosphates and trialkyl phosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkyl polyglycosides, polyglyceryl esters and sugar esters. Particularly preferred surfactants are polysorbates belonging to nonionic surfactants. The amount thereof may be 0.1 to 10% by weight, preferably 0.5 to 5.0% by weight, based on the total weight of the nanoliposome.

The phospholipid, another component used in the preparation of the nanoliposomes of the present invention, is a lipophilic lipid that is used with both affinity lipids and is composed of natural phospholipids (e.g., egg yolk lecithin or soy lecithin, sphingomyelin) Dipalmitoyl phosphatidylcholine or hydrogenated lecithin), preferably lecithin. In particular, unsaturated lecithin or saturated lecithin derived from natural origin extracted from soybean or egg yolk is preferable. Normally, the amount of phosphatidylcholine is 23 to 95% and the amount of phosphatidylethanolamine is 20% or less in natural derived lecithin. In the production of the nanoliposome of the present invention, the amount of the phospholipid to be used is 0.5 to 20.0% by weight, preferably 2.0 to 8.0% by weight based on the total weight of the nanoliposome.

Fatty acid to be used in nano-liposome preparation of the present invention is a higher fatty acid, preferably a C ₁₂ - ₂₂ as a saturated or unsaturated fatty acid of the alkyl chain, e.g., lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid . The amount thereof may be 0.05 to 3.0% by weight, preferably 0.1 to 1.0% by weight, based on the total weight of the nanoliposome.

The water used in the preparation of the nanoliposome of the present invention is generally deionized distilled water, and the amount thereof may be 5.0-40 wt% based on the total weight of the nanoliposome.

The preparation of nanoliposomes can be accomplished through a variety of methods known in the art, but most preferably is made by applying a mixture comprising the ingredients to a high pressure homogenizer. The preparation of a nanoliposome by a high pressure homogenizer can be carried out under various conditions (for example, pressure, number of times, etc.) depending on a desired particle size, and preferably at a high pressure homogenizer So that the nanoliposome can be produced.

The cosmetic composition for inhibiting sebum formation according to the present invention may contain the above-described green tea extract or its fraction in an amount of 0.1 to 20% by weight based on the weight of the nanoliposome.

The present invention also provides a health functional food for inhibiting sebum production comprising a green tea extract or a fraction thereof as an active ingredient.

The health functional food of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and circles for the purpose of inhibiting sebum production.

In the present invention, the term " health functional food " refers to foods manufactured and processed using raw materials or ingredients having useful functions in accordance with Law No. 6727 on Health Functional Foods. Or to obtain a beneficial effect in health use such as physiological action.

The health functional foods of the present invention may contain conventional food additives and, unless otherwise specified, whether or not they are suitable as food additives are classified according to the General Rules for Food Additives approved by the Food and Drug Administration, Standards and standards.

Examples of the items listed in the above-mentioned "food additives" include chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; Natural additives such as persimmon extract, licorice extract, crystalline cellulose, high color pigment and guar gum; L-glutamic acid sodium preparations, noodle-added alkalis, preservative preparations, tar coloring preparations and the like.

For example, the health functional food in the form of tablets may be prepared by granulating a mixture of the green tea extract or the fraction thereof, which is an active ingredient of the present invention, with an excipient, a binder, a disintegrant and other additives in a usual manner, Or the mixture can be directly compression-molded. In addition, the health functional food of the tablet form may contain a mating agent or the like if necessary.

The hard capsule of the capsule type health functional food may be prepared by filling a normal hard capsule with a mixture of the green tea extract or the fraction thereof, which is an active ingredient of the present invention, with an additive such as an excipient. The soft capsule may contain a green tea extract, The fraction may be prepared by mixing a mixture obtained by mixing the fraction with an additive such as an excipient into a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a coloring agent, a preservative and the like, if necessary.

The ring-shaped health functional food can be prepared by molding a mixture of the extract of the present invention, which is an effective ingredient of the present invention, or a fraction thereof with an excipient, a binder, a disintegrant, etc. by a known method, It may be applied to the skin, or the surface may be coated with a substance such as starch or talc.

The granular health functional food may be prepared by granulating a mixture of the extract of the present invention or its fractions with an excipient, a binder, a disintegrant and the like in a granular form by a conventionally known method, And a mating agent.

The health functional food may be a beverage, a meat, a chocolate, a food, a confectionery, a pizza, a ramen, a noodle, a gum, a candy, an ice cream, an alcoholic beverage, a vitamin complex and a health supplement food.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example  1>

Green tea  Alcohol extract and its Fraction  Produce

The collected green tea leaves were washed to remove the salts and extracted with a fermented alcohol which was harmless to the human body. The extracts containing the functional ingredients were prepared and the functional components were separated from the green tea extract by high speed chromatography. The thus prepared green tea extract was used as a sample in the following experiment (see FIG. 1).

< Example  2>

Green tea  Evaluation of cytotoxicity of alcohol extracts

The cytotoxicity of the Hamcho seed extract prepared in Example 1 was evaluated.

In detail, the cell viability of human sebum cells prepared in Example 1 was measured at 48 hours after treatment with human sebum cells (6.5, 12.5, 25, 50, and 100 μg / ml). Cell viability was also measured at 12 and 48 hours after treatment with linoleic acid and the concentration of the present invention (6.5, 12.5, 25, 50, and 100 μg / ml). Linoleic acid is a substance capable of inducing fat production of sebum cells without cytotoxicity.

As a result, as shown in Fig. 2a, no cytotoxicity was observed in all the experimental groups even after 48 hours from the treatment with the Hamcho extract. In addition, as shown in FIG. 2B, the cell survival rate of 100% was observed in the experimental group treated with linoleic acid and the extract of Hamcho seed extract of the present invention at 48 hours, indicating that the extract of Hamcho seed extract of the present invention is safe for skin I could confirm.

< Example  3>

Green tea  Antioxidant activity of alcohol extract

In order to examine the antioxidant activity of the green tea extract prepared in Example 1, the active oxygen scavenging ability of the green tea extract was measured in the sebaceous cells.

In detail, the cells were pretreated with linoleic acid and the extract of the present invention at concentrations of 6.25, 12.5, 25, 50, and 100 μg / ml, treated with H ₂ O ₂ to measure intracellular ROS levels , And a DCFH-DA (2 ', 7'-dichlorodihydrofluorescin diacetate) assay was used to measure intracellular reactive oxygen species. DCFH-DA reacts with reactive oxygen species in the cell to produce DCF (dichlorofluorescein). By measuring the fluorescence generated by introducing the reagent into the cell, active oxygen species in the cell can be measured.

As a result, as shown in FIG. 3, it was confirmed that the active oxygen generated by H ₂ O ₂ was inhibited by the extract in a concentration-dependent manner.

< Example  4>

Green tea  Inhibitory activity of sebum production of alcohol extracts

<4-1> Green tea  Inhibitory activity on the triglyceride formation of alcohol extracts

In order to examine the inhibitory activity on the production of triglyceride by the Hamcho extract of Example 1, 6.25, 12.5, 25, 50, and 100 μg / ml of linoleic acid and human leishmania extract of the present invention were added to human sebum cells, To induce triglyceride production, and the triglyceride content was determined using a triglyceride kit.

As a result, as shown in FIG. 4, it was confirmed that the production of triglyceride was inhibited in sebaceous cells depending on the treatment concentration of the extract of the present invention. Thus, It could be useful as an adjustable cosmetic material.

<4-2> Green tea  Foam of alcohol extract Intracellular  Fat accumulation inhibitory activity

In order to confirm whether the extract of Example 1 could inhibit fat accumulation in sebaceous cells, human sebaceous gland cells were treated with linoleic acid at a concentration of 100 μg / ml and 100 μg / ml of the extract of the present invention After 24 hours of treatment, lipid changes in the cells were observed through oily red O staining, a local staining reagent.

As a result, as shown in FIG. 5, it was confirmed that the accumulation of fat in human sebum cells was significantly inhibited in the experimental group treated with the green tea extract of the present invention.

<4-3> Green tea  The effect of alcohol extract on the expression of fat-related factors

In order to investigate the effect of the Hamcho seed extract prepared in Example 1 on the expression of SREBP-1 PPARα, PPARγ and C / EBPβ, lipogenesis-related factors, human liposomal cells were treated with linoleic acid (100 μg / ml) The amount of SREBP-1 PPARα, PPARγ, and C / EBPβ expressed in the cells was analyzed by Western blot analysis after treating the green tea extract of the present invention by concentration.

SREBP-1 and C / EBPβ, which are regulators of lipid enzymes, are increased by external stimuli to regulate the maturation of sebaceous glands and increase the expression of the PPAR gene, which stimulates lipid formation in the epidermis, It is known.

As a result, as shown in FIG. 6, the protein expression of SREBP-1 PPARα, PPARγ, and C / EBPβ related to intracellular fat production in the experimental group treated with the green tea extract of the present invention was found to be reduced I could. Based on these results, it was determined that the green tea extract of the present invention inhibited the expression of these factors and consequently inhibited lipogenesis in sebaceous cells.

< Example  5>

Green tea  Assessment of sebum improvement efficacy of alcohol extracts Human test

In order to clinically confirm whether or not the extract of the green tea produced by Example 1 was actually effective to improve the sebum, 10 kinds of green tea extracts of the present invention (100 μg / ml) was applied to the skin, and the degree of decrease in oiliness was measured at the point of 1 week and 2 weeks after the application to the skin.

As a result, as shown in FIG. 7, it was confirmed that the oil content of the skin surface was reduced in both regions compared with the use of the extract of the present invention.

In conclusion, it was confirmed that the green tea extract of the present invention had an effect of reducing intracellular fat production through reduction of expression of SREBP-1, PPARα, PPARγ and C / EBPβ proteins in sebum cells inducing lipogenesis, In clinical trials based on in vitro experiments, it was found that the extracts of Korean mackerel juice were useful as functional materials for cosmetic products.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (7)

A cosmetic composition for inhibiting sebum production, comprising a green tea extract or a fraction thereof as an active ingredient. The method according to claim 1,
The green tea extract may be prepared by extracting a dried green tea powder with a solvent of ethanol (ethanol); Filtering the extracted filtrate, and then concentrating the filtrate by distillation under reduced pressure; And a step of lyophilizing and pulverizing the concentrated filtrate to obtain a cosmetic composition for inhibiting sebum formation. The method according to claim 1,
Extracting the fractions by adding a spirit (ethanol) solvent to the dried green tea powder; Filtering the extracted filtrate, and then concentrating the filtrate by distillation under reduced pressure; Performing high-speed chromatography on the concentrate to separate the functional fractions; And a step of lyophilizing and separating the separated fractions to obtain a cosmetic composition for suppressing sebum formation. The method according to claim 1,
The cosmetic composition for inhibiting sebum formation, wherein the green tea extract or the fraction thereof is contained in an amount of 0.001 to 10% by weight based on the dry weight of the composition. The method according to claim 1,
The green tea extract or its fractions have antioxidant activity; And a function of controlling excess sebum through inhibition of triglyceride formation and suppression of fat accumulation in sebaceous cells. The method according to claim 1,
Wherein the green tea extract or the fraction thereof inhibits lipogenesis in sebum cells through a mechanism of decreasing protein expression of SREBP-1 PPAR [alpha], PPAR [gamma], and C / EBP [beta]. 7. The method according to any one of claims 1 to 6,
The composition may be in the form of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Wherein the composition is formulated into one selected from the group consisting of cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, lipstick, makeup base, foundation, press powder and loose powder.

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