KR20130121242A - Dna oligomer that binds as an antisense to the capra hircus octamer-binding transcription factor 4 -oct-4 mrna - Google Patents

Dna oligomer that binds as an antisense to the capra hircus octamer-binding transcription factor 4 -oct-4 mrna Download PDF

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KR20130121242A
KR20130121242A KR1020120044269A KR20120044269A KR20130121242A KR 20130121242 A KR20130121242 A KR 20130121242A KR 1020120044269 A KR1020120044269 A KR 1020120044269A KR 20120044269 A KR20120044269 A KR 20120044269A KR 20130121242 A KR20130121242 A KR 20130121242A
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oct
mrna
octamer
transcription factor
dna
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KR1020120044269A
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Korean (ko)
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김승찬
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김승찬
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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Abstract

The present invention relates to a method for suppressing the expression of Capra hircus octamer-binding transcription factor 4-Oct-4 proteins which are related to inflammation by making DNA fragments which complementarily bind to mRNA generated during an expression of Capra hircus octamer-binding transcription factor 4-Oct-4 genes. The present invention enables easy reduction of expression levels of the proteins in in vitro cultured cells or tissues or in vivo by making the DNA oligomers with 10 base pair or more, which complementarily bind to mRNA of Capra hircus octamer-binding transcription factor 4 (Oct-4) proteins.

Description

DNA oligomers that binds as an anti-binding that binds to an anti-binding that binds to an anti-binding that binds to an anti-binding that binds to an anti-binding method that binds complementarily to mRNA for a 4- to 4--4.

The present invention is a method of inhibiting the expression of the protein by making a DNA fragment complementary to the mRNA produced during the expression of the Capra hircus octamer-binding transcription factor 4 -Oct-4 gene associated with the inflammatory response.

Induction of Retrovirus-mediated Four Transcription Factors Oct3 / 4 (164177), Sox2, C-Myc (190080), and Klf4 (602253) for induced pluripotent stem (IPS) cells Fbx15, and subsequent selection in mouse fibroblasts (609093) expression (Takahashi and Yamanaka in 2006). These IPS cells are similar to cell proliferation and teratoma formation in embryonic stem (ES) forms, hereinafter called Fbx15 IPS cells, but they differ in terms of gene expression and DNA methylation patterns, and failing to produce adult chimeras. Okita et al. (2007) showed increased gene expression as ES cells and their selection for DNA methylation patterns and Nanog (607937) expression results in germline-controlled IPS cells compared to Fbx15 IPS cells. Four transgenes were actively silenced in Nanog IPS cells.

Synthesis and Administration of Antisense DNA to mRNA to Reduce Capra hircus octamer-binding Transcription Factor 4 -Oct-4

Eighteen DNA nucleotides are synthesized for the front, middle and back of the gene sequence for mRNA. Dilute it and put it into cells or tissues.

1. The knock down effect can be augmented by looking at interference using DNA that is more stable than the conventional micro RNA method.

2. By regulating the expression level of Capra hircus octamer-binding transcription factor 4 -Oct-4, various amyloid beta infiltrations and their systems can be controlled and used to prevent apoptosis and dementia.

Figure 1 Figure of complementary 18 base pair generation method for mRNA coding region of Capra hircus octamer-binding transcription factor 4 -Oct-4

The reason for using 18 base pairs in FIG. 1 is that there are 3 billion base pairs in the human gene. In order to induce specific binding in these base pairs, there must be at least 14 specific lengths of nucleotides, i.e., one in the number of fourteenth-squared cases. Therefore, 18base pair was used more safely. By targeting these DNA oligomers at the front, middle, and end of the mRNA, it is possible to appropriately control the expression level by selecting the one with the highest knockdown efficiency or the lowest efficiency. At this time, since 18base pair is a short strand, it can be ordered like PCR primer synthesis.

Claims (4)

How to reduce the expression level of the protein in cultured cells, tissues and in vivo by constructing DNA oligomers of 10 base pairs or more complementary to mRNA of Capra hircus octamer-binding transcription factor 4 (Oct-4) protein Or its application The method of claim 1 wherein the DNA oligomer sequence of 18 base pair
3'-aaacggtt cgaggatttc-5 '(complementary DNA strand to the front)
3'-cgaaacgt cgagtcaaag-5 '(complementary DNA strand to the middle)
3'-cgggcttt ctctttcgcc-5 '(complementary DNA strand to the back) The method for preparing DNA oligomers complementary to Oct-4 mRNA is based on the use of PCR primers. The experimental device or method of claim 1, wherein the expression level of the protein is controlled through a DNA oligomer complementary to Oct-4 mRNA, thereby controlling the signaling system, cell cycle, and cell differentiation associated with the protein.
KR1020120044269A 2012-04-27 2012-04-27 Dna oligomer that binds as an antisense to the capra hircus octamer-binding transcription factor 4 -oct-4 mrna KR20130121242A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020120044269A KR20130121242A (en) 2012-04-27 2012-04-27 Dna oligomer that binds as an antisense to the capra hircus octamer-binding transcription factor 4 -oct-4 mrna

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020120044269A KR20130121242A (en) 2012-04-27 2012-04-27 Dna oligomer that binds as an antisense to the capra hircus octamer-binding transcription factor 4 -oct-4 mrna

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KR20130121242A true KR20130121242A (en) 2013-11-06

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR200481762Y1 (en) 2016-06-01 2016-11-07 주식회사 가나오케이 Ceiling fan
KR20190025409A (en) * 2017-09-01 2019-03-11 엘지전자 주식회사 Flow generator

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR200481762Y1 (en) 2016-06-01 2016-11-07 주식회사 가나오케이 Ceiling fan
KR20190025409A (en) * 2017-09-01 2019-03-11 엘지전자 주식회사 Flow generator

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