KR20130023992A - Antibacterial composition for preventing growth of pathogenic microorganism of livestock comprising essential oil of chaemaecyparis obtusa as active ingredient - Google Patents
Antibacterial composition for preventing growth of pathogenic microorganism of livestock comprising essential oil of chaemaecyparis obtusa as active ingredient Download PDFInfo
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- A—HUMAN NECESSITIES
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- A23K20/195—Antibiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
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Abstract
Description
본 발명은 편백 정유를 유효성분으로 하는 가축 병원성 세균 억제용 항균 조성물, 특히 가축의 호흡기 질환을 유발하는 세균은 액티노바실러스 플로르뉴모니아(Actinobacillus pleuropneumoniae) 또는 파스투렐라 물토시다(Pasteurella multocida)를 억제하는 항균 조성물 및 그를 이용한 가축사료에 관한 것이다.
The present invention is an antimicrobial composition for inhibiting livestock pathogenic bacteria with an active ingredient of cypress essential oil, in particular, a bacterium causing respiratory diseases in cattle is Actinobacillus It relates to an antimicrobial composition that inhibits pleuropneumoniae ) or Pasteurella multocida and livestock feed using the same.
우리나라 남부지방에 널리 분포하고 있는 측백나무과의 상록교목인 편백(扁柏: Chamaecyparis obtusa Sieb. et Zucc)는 피톤치드라는 성분을 공기 중에 발산한다. 피톤치드(phytoncide)란 식물이 해충이나 미생물로부터 자신을 방어하기 위하여 만들어 내는 살균성 물질의 총칭으로, 편백의 피톤치드, 즉 편백 가지와 잎을 이용한 정유 또는 침출수에 여러 가지 미생물 억제능을 가진 것으로 밝혀져 있다. Chamaecyparis , an evergreen tree of the Cypressaceae, widely distributed in the southern part of Korea obtusa Sieb. et Zucc) emit a component called phytoncides into the air. Phytoncide is a generic name of a bactericidal substance produced by plants to protect themselves from pests and microorganisms. Phytoncide is known to have various microbial inhibitory effects on essential oils or leachate using cypress branches and leaves.
한국공개특허 제2005-0019649호는 편백 정유를 황색포도상구균, 리스테리아균 및 레지오넬라균에 대한 항미생물적 제재로 이용하는 방법을 개시하고 있다.Korean Laid-Open Patent Publication No. 2005-0019649 discloses a method of using a white protein essential oil as an antimicrobial agent against Staphylococcus aureus, Listeria and Legionella.
또한 한국공개특허 제2008-0112664호는 편백 오일이 충치의 주 원인균인 ㅅ스트렙토코커스 뮤탄스(Streptococcus mutans)의 생육을 억제하므로, 치약이나 구강용 제품에 이용될 수 있음을 개시하고 있다.In addition, Korean Laid-open Patent No. 2008-0112664 discloses that cypress oil inhibits the growth of Streptococcus mutans , which is the main cause of tooth decay, and thus can be used in toothpaste or oral products.
또한 한국공개특허 제2009-0038648호는 편백 오일이 여드름의 주 원인균인 프로피오니박테리움 아크네스(Propionibacterium acnes) 또는 스타필로코커스 에피더미디스(Staphylococcus epidermidis)의 성장 저해능을 가지므로, 여드름 피부용 화장료 조성물에 사용될 수 있음을 개시하고 있다.In addition, Korea Patent Publication No. 2009-0038648 is a PROFIBUS sludge cypress five hundred and twelve weeks cause of acne tumefaciens arc Ness (Propionibacterium Acnes ) or Staphylococcus epidermidis ( Staphylococcus epidermidis ) has the ability to inhibit the growth, it is disclosed that it can be used in cosmetic compositions for acne skin.
또한 한국공개특허 제2009-0039458호는 편백 오일이 비듬의 주 원인균인 피티로스포름 오발레(Pityrosporum Ovale)의 성장 저해능을 가지므로, 비듬의 치료에 이용될 수 있음을 개시하고 있다.In addition, Korean Patent Laid-Open Publication No. 2009-0039458 discloses that cypress oil may be used for the treatment of dandruff because it has a growth inhibitory ability of Pityrosporum Ovale , which is a main cause of dandruff.
그러나 이러한 편백 정유가 가축의 병원성 세균을 억제하는 효과가 있다는 점에 대해서는 알려진 바가 없고, 특히 가축의 호흡기 질환을 유발하는 세균은 액티노바실러스 플로르뉴모니아(Actinobacillus pleuropneumoniae) 또는 파스투렐라 물토시다(Pasteurella multocida)를 억제하는 점에 대해서는 알려지지 않아, 이러한 편백 정유가 가축의 세균성 질환 예방을 위한 항균 조성물로서 사용될 수 있다는 점에 대해서는 밝혀진 바가 없다.
However, it is not known that these protein essential oils inhibit the livestock pathogenic bacteria, and in particular, the bacteria causing respiratory diseases in the livestock are actinobacillus. It is not known to inhibit pleuropneumoniae ) or Pasteurella multocida , and it is not known that such a protein essential oil can be used as an antimicrobial composition for the prevention of bacterial diseases in livestock.
본 발명의 목적은 가축의 병원성 세균을 억제하는 항균 조성물, 특히 가축의 호흡기 질환을 유발하는 세균은 액티노바실러스 플로르뉴모니아(Actinobacillus pleuropneumoniae) 또는 파스투렐라 물토시다(Pasteurella multocida)를 억제하는 항균 조성물을 제공하는 것이다.An object of the present invention is an antimicrobial composition that inhibits pathogenic bacteria in livestock, in particular, bacteria that cause respiratory diseases in cattle are actinobacillus florneumoniae ( Actinobacillus) pleuropneumoniae ) or Pasteurella multocida to provide an antimicrobial composition.
본 발명의 다른 목적은 가축 병원성 세균 억제용 항균 조성물을 가축 축사에서 분무 또는 기화시키는 것을 특징으로 하는 가축의 병원성 세균 유래 질병의 예방방법을 제공하는 것이다.Another object of the present invention is to provide a method for preventing pathogenic bacteria-derived diseases of a livestock, characterized in that the spraying or evaporating the antimicrobial composition for animal pathogenic bacteria in a livestock barn.
본 발명의 또다른 목적은 가축 병원성 세균 억제용 항균 조성물을 포함하는 가축 사료를 제공하는 것이다.
Another object of the present invention to provide a livestock feed comprising an antimicrobial composition for inhibiting livestock pathogenic bacteria.
본 발명의 가축 병원성 세균 억제용 항균 조성물은 편백 정유를 유효성분으로 포함하여 이루어진다.Antimicrobial composition for inhibiting livestock pathogenic bacteria of the present invention comprises a protein essential oil as an active ingredient.
본 발명의 가축 병원성 세균 억제용 항균 조성물에서, 상기 가축 병원성 세균은 호흡기 질환을 유발하는 세균인 것을 특징으로 한다.In the antimicrobial composition for inhibiting livestock pathogenic bacteria of the present invention, the livestock pathogenic bacteria is characterized in that the bacteria causing respiratory diseases.
본 발명의 가축 병원성 세균 억제용 항균 조성물에서, 상기 호흡기 질환을 유발하는 세균은 액티노바실러스 플로르뉴모니아(Actinobacillus pleuropneumoniae) 및 파스투렐라 물토시다(Pasteurella multocida) 중에서 선택된 어느 하나 이상인 것을 특징으로 한다.In the antimicrobial composition for inhibiting livestock pathogenic bacteria of the present invention, the bacterium causing the respiratory disease is Actinobacillus pleuropneumoniae) and La paseuturel the water Toshi (Pasteurella multocida ) characterized in that at least one selected from.
본 발명의 가축 병원성 세균 억제용 항균 조성물에서, 상기 편백 정유는 초임계 추출된 것을 특징으로 한다.In the antimicrobial composition for inhibiting livestock pathogenic bacteria of the present invention, the essential protein is characterized in that the supercritical extraction.
본 발명의 가축 병원성 세균 유래 질병의 예방방법은, 상기 가축 병원성 세균 억제용 항균 조성물을 가축 축사에서 분무 또는 기화시키는 것을 특징으로 한다.The method of preventing livestock pathogenic bacteria-derived diseases of the present invention is characterized in that the antimicrobial composition for inhibiting livestock pathogenic bacteria is sprayed or vaporized in a livestock barn.
본 발명의 가축 사료는, 상기 가축 병원성 세균 억제용 항균 조성물을 포함하여 이루어진다.Livestock feed of the present invention comprises the antimicrobial composition for inhibiting livestock pathogenic bacteria.
본 발명의 가축 사료는, 상기 가축 병원성 세균 억제용 항균 조성물을 함침시킨 편백잎 분말을 0.1 ~ 5 중량% 포함하는 것을 특징으로 한다.
Livestock feed of the present invention is characterized in that it comprises 0.1 to 5% by weight of cypress leaf powder impregnated with the antimicrobial composition for inhibiting livestock pathogenic bacteria.
본 발명은 가축의 병원성 세균, 특히 가축의 호흡기 질환을 유발하는 세균은 액티노바실러스 플로르뉴모니아(Actinobacillus pleuropneumoniae) 또는 파스투렐라 물토시다(Pasteurella multocida)를 억제하는 항균 조성물을 제공함으로써, 상기 항균 조성물을 가축 축사에서 분무 또는 기화시키는 것을 특징으로 하는 가축의 병원성 세균 유래 질병의 예방방법을 제공하고, 또한 상기 항균 조성물을 포함하는 가축 사료를 제공한다.
The present invention is a pathogenic bacterium of the livestock, in particular, a bacterium causing respiratory diseases of the livestock is Actinobacillus ( Actinobacillus Providing an antimicrobial composition that inhibits pleuropneumoniae ) or Pasteurella multocida , thereby providing a method for preventing pathogenic bacterial diseases of livestock, characterized in that the antimicrobial composition is sprayed or vaporized in a livestock barn. It provides a livestock feed comprising the antimicrobial composition.
이하, 본 발명을 하기 실시예를 참조하여 더욱 구체적으로 설명하기로 한다. 다만, 하기 실시예는 본 발명의 이해를 돕기 위한 것일 뿐, 본 발명의 범위가 하기 실시예에 한정되는 것은 아니다.
Hereinafter, the present invention will be described more specifically with reference to the following examples. However, the following examples are provided only to aid understanding of the present invention, and the scope of the present invention is not limited to the following examples.
공시 세균Disclosure Bacteria
전남소재 양돈장에서 분리된 Pasteurella , multocida 13개 야외분리주, Actinobacillus pleuropneumoniae 13개 야외분리주에 대해 편백수의 항생제 감수성 평가를 하였다.
Pasteurella , multocida isolated from pig farm in Jeonnam 13 outdoor isolates, Actinobacillus pleuropneumoniae Antimicrobial susceptibility evaluation of cyanobacteria was performed in 13 field isolates.
실험예Experimental Example 1: One: ActinobacillusActinobacillus pleuropneumoniaepleuropneumoniae 에 대한 항생제 감수성 검사Antibiotic Susceptibility Testing
선택배지를 96 microwell plate에 140 ㎕씩 넣은 후 최초의 well에 편백 정유를 140 ㎕를 넣어 2배수 계단희석하였다. Actinobacillus pleuropneumoniae 세균을 McFarland No. 0.5에 맞추어(1.5 *108/ml) 준비한 후 각각의 세균140 ㎕를 96 microwell plate에 접종하여 37℃, 5% CO2 incubator 에서 24시간 배양하였다. 배양 24 시간 후 계단 희석된 편백수의 농도별 세균 증식 유무를 고체 선택 배지에 도말, 배양하여 증식 유무를 통해 편백수의 세균 증식을 억제하는 최소 농도로 결정하였다. 140 μl of the selective medium was added to a 96 microwell plate, and 140 μl of the cypress essential oil was added to the first well, followed by two-fold step dilution. Actinobacillus pleuropneumoniae Bacteria in McFarland No. After preparing to 0.5 (1.5 * 10 8 / ml), 140 μl of each bacterium was inoculated into a 96 microwell plate and incubated in 37 ° C. and 5% CO 2 incubator for 24 hours. After 24 hours of cultivation, the presence or absence of bacterial growth by concentration of the stepwise diluted cypresses was determined by smearing and culturing in a solid selection medium to determine the minimum concentration that inhibited the bacterial growth of cyanobacteria through proliferation.
Actinobacillus pleuropneumoniae 13개 야외주에 대한 최소억제농도를 표 1에 나타내었다. Actinobacillus pleuropneumoniae The minimum inhibitory concentrations for the thirteen outdoor stocks are shown in Table 1.
표 1에서와 같이 총 13개의 분리주는 2-1의 최소 억제농도에서 8개 분리주가 증식이 억제 되었으며 최소 억제농도 2- 8 에서 2개 분리주가 억제 되었다. 이러한 결과를 동일한 야외 분리주를 국내에서 치료제로 사용되고 있는 페니실린을 포함한 22개의 항생제의 표준 사용농도에서의 항생제 감수성 결과와 비교하여 표 2에 나타내었다. Was a total of 13 separate to two MICs grown in separate eight weeks of -1 suppressed as shown in Table 12 The minimum inhibitory concentration-weeks was inhibited in two separate 8. These results are shown in Table 2 by comparing the antibiotic susceptibility results with the standard concentrations of 22 antibiotics, including penicillin, which is used as a therapeutic agent in Korea.
클리코사이드Cliccoside
트리메토프림Trimethoprim
트리메토프림(Trimethoprim (
SXTSXT
))
표 2에 나타난 봐와 같이 국내에서 분리된 흉막 폐렴 원인균 Actinobacillus pleuropneumoniae 는 플로르페니콜에서 9개 분리주가, 아미카닌에서 6개 분리주가 증식이 억제되는 감수성을 나타 내었고 대부분의 항생제에 대해서는 내성을 나타내었다. 편백 정유의 2-1의 최소 억제농도에서 확인된 8주의 증식억제 능력은 항생제 감수성 비교할 때 내성 항생제의 대체제로서 선택할 수 있는 가능성을 확인할 수 있었다.
As shown in Table 2, actinobacillus pleuropneumoniae, a pleural pneumococcal bacterium isolated from Korea, showed susceptibility to inhibiting proliferation in 9 isolates from florfenicol and 6 isolates from amicanin, and resistant to most antibiotics. It was. The 8-week antiproliferative capacity identified at a minimum inhibitory concentration of 2 -1 of cypress essential oil was confirmed to be a viable alternative to resistant antibiotics when compared to antibiotic susceptibility.
실험예Experimental Example 2: 2: PasteurallPasteurall multocidamultocida 에 대한 항생제 감수성 검사Antibiotic Susceptibility Testing
실험예 1과 동일하게 실험을 실시하되 세균만 Pasteurella , multocida 세균을 사용하였다.Experiment was carried out as in Experimental Example 1, but only bacteria Pasteurella , multocida Bacteria were used.
Pasteurall multocida 13개 야외주에 대한 최소억제농도를 표 2에 나타내었다. Pasteurall multocida The minimum inhibitory concentrations for the thirteen outdoor stocks are shown in Table 2.
표 3에서 Pasteurall multocida 13개 야외주에 대한 편백수의 최소억제농도를 평가한 결과, 총 13개의 분리주는 2-1의 최소 억제농도에서 13개 분리주가 증식이 억제 되었으며 최소 억제농도 2- 8 에서 2개 분리주가 억제 되었다. 이러한 결과를 동일한 야외 분리주를 국내에서 치료제로 사용되고 있는 페니실린을 포함한 22개의 항생제의 표준 사용농도에서의 항생제 감수성 결과와 비교하여 표 4에 나타내었다. Pasteurall in Table 3 multocida 13 evaluation results of the minimum inhibitory concentration of cypress for open shares, a total of 13 separate to 2-1 were isolated from the 13 weeks minimum inhibitory concentration inhibiting the growth minimal inhibitory concentrations of 2-suppressed Removing 2-8 weeks It became. These results are shown in Table 4 in comparison with the antibiotic susceptibility results at the standard concentration of 22 antibiotics, including penicillin, which is used as a therapeutic agent in Korea.
클리코사이드Amino
Cliccoside
트리메토프림Sulfonylamide +
Trimethoprim
트리메토프림(SXT)Sulfamethoxazole
Trimethoprim (SXT)
Etc
표4에 나타난 봐와 같이 국내에서 분리된 폐렴 원인균 Pasteurall multocida는 플로르페니콜에서 9개 분리주가, 아미카닌에서 6개 분리주가 증식이 억제되는 감수성을 나타냈으며 대부분의 항생제에 대해서는 내성을 나타내었다. 편백 정유의 2-1의 최소 억제농도에서 확인된 전체 균주 13주에 대한 증식억제 능력은 항생제 감수성 비교할 때 내성 항생제의 대체제로서 선택할 수 있는 가능성을 확인 할 수 있었다.
As shown in Table 4, isolated pneumonia-causing bacteria in Korea Pasteurall multocida was susceptible to inhibition of proliferation in 9 isolates from florfenicol and 6 isolates from amicanin and resistant to most antibiotics. The antiproliferative ability of all strains identified at the minimum inhibitory concentration of 2 -1 of cypress essential oil was found to be an alternative to resistant antibiotics when compared to antibiotic susceptibility.
상기 실험예 1 및 2의 결과를 종합해 볼 때 편백 정유는 돼지에서 분리된 Actinobacillus pleuropneumoniae , Pasteurall multocida에 대하여 종래 사용중인 22 종의 항생제와 비교하였을 때 감수성이 현저히 뛰어나, 종래 항생제를 대체할 수 있는 천연 항균 조성물로 사용될 수 있다고 평가되었다. Putting together the results of Experimental Examples 1 and 2 , the protein of essential oil is Actinobacillus pleuropneumoniae , Pasteurall isolated from pigs. Compared with 22 antibiotics in multocida , multocida has excellent sensitivity and was evaluated as a natural antimicrobial composition that can replace conventional antibiotics.
Claims (7)
Antimicrobial composition for inhibiting livestock pathogenic bacteria with an active ingredient of protein in essential oil.
The antimicrobial composition for inhibiting livestock pathogenic bacteria according to claim 1, wherein the livestock pathogenic bacteria are bacteria that cause respiratory diseases.
The method of claim 2, wherein the bacterium causing the respiratory disease is Actinobacillus pleuropneumoniae) and La paseuturel the water Toshi (Pasteurella multocida ) antimicrobial composition for inhibiting livestock pathogenic bacteria, characterized in that any one or more selected from.
The antimicrobial composition for inhibiting livestock pathogenic bacteria according to claim 1, wherein the protein is essential oil.
A method for preventing pathogenic bacteria-derived diseases of a livestock, characterized by spraying or vaporizing the antimicrobial composition for inhibiting livestock pathogenic bacteria of any one of claims 1 to 4.
Claims 1 to 4 livestock feed comprising an antimicrobial composition for inhibiting livestock pathogenic bacteria.
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CN103704514A (en) * | 2013-12-23 | 2014-04-09 | 山东新希望六和集团有限公司 | Feed for treating porcine contagious pleuropneumonia and preparation method of feed |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103704514A (en) * | 2013-12-23 | 2014-04-09 | 山东新希望六和集团有限公司 | Feed for treating porcine contagious pleuropneumonia and preparation method of feed |
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