KR20110032715A - Antiaging cosmetic compositions and method for producing thereof - Google Patents
Antiaging cosmetic compositions and method for producing thereof Download PDFInfo
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- KR20110032715A KR20110032715A KR1020090090344A KR20090090344A KR20110032715A KR 20110032715 A KR20110032715 A KR 20110032715A KR 1020090090344 A KR1020090090344 A KR 1020090090344A KR 20090090344 A KR20090090344 A KR 20090090344A KR 20110032715 A KR20110032715 A KR 20110032715A
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
In addition to eliminating problems such as skin diseases due to irritation or troubles of sensitive skin, it is possible to obtain antioxidant function, cell proliferation and activation, collagen synthesis enhancement, and moisturizing function. When extracting by fermenting at least any one, but extracting by mixing two or more, it provides an anti-aging cosmetic composition and a method for producing the same comprising a herbal medicine fermented extract extracted by mixing, fermenting each in the same weight ratio.
Description
The present invention relates to an anti-aging cosmetic composition and a method for manufacturing the same, and more particularly, to eliminate problems such as skin diseases or problems with sensitive skin due to irritation, as well as to enhance antioxidant function, cell proliferation and activation, collagen synthesis, moisturizing It relates to an anti-aging cosmetic composition exhibiting a function and a manufacturing method thereof.
The desire for beauty is the instinct given to man. In particular, efforts to maintain skin and maintain youthfulness from the desire to have beautiful skin have been progressed variously with the history of mankind.
In the case of such skin care, there are two aspects that must be considered. The first is to meet the propensity of beauty-oriented people, and the second is to meet its value as a means of protecting one's body from the outside.
Looking at the traces of skin beauty in Korea's history, wormwood and garlic, which appeared in the Dangun mythology of Gojoseon, were also a way to survive, but the ingredients are not related to the skin protection function through smooth metabolism.
In addition, people in the towns who lived in the cold regions of the northeastern part of the Korean peninsula used pig oil to prevent skin damage, frostbite, etc., caused by cold, and Malgales washed with urine to whiten the skin.
Today's cremation began only in the Three Kingdoms era, and the technology of making paints developed in the Three Kingdoms period led to the emergence of the rendezvous, which led to the development of cremation as it is today.
Later, during the Goryeo Dynasty, the face was embellished with white powder. During the Joseon Dynasty, the face was made using cucumbers and honey. Especially, in the "The Union Book," various hair types, ten ways of drawing eyebrows, and various lips How to take a rouge is recorded.
Korea traditionally puts the standard of beauty in the spirit and fleshly ideology, and emphasized the harmony of the external beauty with the internal beauty. In particular, the skin not only has a close relationship through blood donation and meridians, but also directly connected to each blood donation of the five Jangyuk Yuk so that the state of mind and body was considered as a mirror. Therefore, fine skin or smooth skin, which is the standard of Korean traditional beauty, is directly related to the health of the person.
Recently, the use of herbal medicines is increasing in the cosmetic field in order to reflect such beauty standards. In the case of such herbal cosmetics, it is known that the moisturizing power, anti-aging function and antioxidant effects are excellent, and research on cosmetic compositions using herbal medicine extracts is being actively conducted.
However, in the case of herbal cosmetic compositions using herbal extracts extracted by conventional methods, the effects of whitening, wrinkle improvement, antioxidants, etc. are insignificant. It is true.
On the other hand, the fermentation broth extract does not use any other solvent, and if the sugar component in the herbal medicine is changed to alcohol through fermentation and aging process, the active ingredients such as flavonoids, vitamin C, vitamin P, amino acids, catechins in the herbal medicine By increasing the extraction rate, it is known that much superior cosmetic effects can be obtained.
In Korea, Hallasan has diverse geographic or geopolitical characteristics, including various plant groups in South and South Korea as continents, a plant group commonly distributed throughout China and Japan, a plant group in tropical or subtropical origin, and a plant group differentiated in Jeju Island or Japan. Are distributed.
Hallasan is vertically distributed from lowland vegetation to lowland highland or alpine plants according to the low to normal environmental gradient.
Moreover, among the plants distributed in the highlands of Mt. Halla, continental plants in Mt. Baekdu, Manchuria, Siberia, and Mongolia are distributed, and many of them are specialized for long-term isolation.
Therefore, the fermentation or aging of various specialty herbal medicines adapting to the wild environment of the mountainous region of Jeju Island and applying the extract to the cosmetics, the dog for a new concept cosmetic composition that can increase the moisturizing power without irritating the skin The feet are desperately needed.
The present invention has been made in view of the background art, it is safe to the skin, there is no problem of causing skin inflammation, and anti-aging cosmetic composition excellent in antioxidant, cell activation through cell proliferation, collagen synthesis enhancement and moisturizing function and its manufacturing method The purpose is to provide.
In addition, the present invention can not only anticipate the anti-aging effect within a short time, but another object of the present invention is to provide an anti-aging cosmetic composition and a method for producing the same that can ensure the persistence of the effect.
The anti-aging cosmetic composition proposed by the present invention is extracted by fermenting at least one of yangyang, thyme, non-tree, rape blossoms, scabies, when extracted by mixing two or more at the same weight ratio, respectively, extracted by fermentation It consists of Chinese herbal fermented extract.
The herbal fermented extract may be contained in a range of 0.01 to 10 parts by weight based on 100 parts by weight of the solvent as a dry weight.
Here, the medicinal herb fermentation extract is inoculated with yeast (Saccharomyces cerevisiae 11201) inoculated by fermentation at 30 ℃ to 60 ℃ for 48 hours, or after fermentation for 3 hours at 40 ℃ to 50 ℃ including the process of extraction It may be prepared in the form.
And, the solvent may be carried out with one or more selected from the group consisting of distilled water, ethanol, methanol, hydrous ethanol, hydrous propylene glycol, hydrous butylene glycol, glycerin. In this case, the hydrous butylene glycol is preferably applied to a 30% hydrous solution.
The herbal fermented extract according to the present invention can be implemented in formulations such as astringent cosmetics, softening cosmetics, lotions, creams, eye creams, essences, foundations and the like.
According to the anti-aging cosmetic composition according to the present invention, it is not irritating, safe for the skin, and prevents the occurrence of various skin diseases, as well as anti-oxidation, activation through cell proliferation, collagen synthesis enhancement and moisturizing ability, the skin aging prevent.
Furthermore, the anti-aging cosmetic composition of the present invention exhibits advantages such as not only the effect appears in a short time but also lasts for a long time.
Next, a preferred embodiment of the anti-aging cosmetic composition according to the present invention will be described in detail.
The anti-aging cosmetic composition proposed by the present invention is characterized in that it comprises a herbal medicine fermented extract obtained by adding and culturing the herbal medicine in the yeast culture as an active ingredient. Here, in the case of the herbal medicine fermentation extract, it may be carried out in a variety of 0.0001 to 100% by weight.
When the herbal medicine fermented extract obtained by fermenting the herbal medicine according to the present invention is applied to the cosmetic composition, the extraction rate of the active ingredient content of flavonoids, vitamin C, vitamin P, amino acids, catechins, etc. contained in the herbal medicine increases, thus preventing wrinkles and the like. It is excellent and safety to skin is also ensured.
Anti-aging cosmetic composition according to the present invention is a herbal medicine fermented extract as an active ingredient. The medicinal herb fermentation extract is obtained by adding and culturing the medicinal herb during yeast culture.
As described above, the herbal fermentation extract added with yeast may be obtained using at least one of yangyang, thyme, non-tree, rape blossoms, and stamens. At this time, the raw materials of the herbal medicine fermentation extract is preferably obtained by mixing in the same weight ratio.
Zingiber mioga is a ginger plant native to tropical Asia. The rhizome stretches sideways and covered with scaly leaves. The leaves are lanceolate or long ellipse shape, and the bottom part is leaf house, which is wrapped around each other and grows into a stem shape, reaching 40 ~ 100cm in height. Flower is yellow in August-October, 5cm in diameter, and long oval-shaped flower runs in stalk. The stalk is wrapped in scaly leaves at the end of the rhizome, 5 ~ 7cm long. Po is narrow egg-shaped and pointed at the end. Calyx is tubular, corolla is divided into three. Lip petals split into three, the largest of the split pieces, with one operation. The stalk is edible before flowering and the stem is edible before spring.
Herbal medicine is mainly used as a medicinal herb of the root stem and seeds, the root stem is used to treat women's menstrual irregularities and white crabs, have an antitussive effect, expectoration, boils and ocular hyperemia. Seeds are taken by adding sugar and water when the stomachache is severe.
The thyme (Thymus quinquecostatus) is a plant of the family Lamiaceae grows in high mountain peaks of Korea, Japan, China, Mongolia, India, etc. or rocky seashore, the origin is Korea. It is 3 ~ 15cm high, and main stem spreads out on the ground, young branches stand at an angle, and smell. The leaves are opposite, long oval or lanceolate, 5-12mm long, 3-8mm butterfly. There is a preemption on both sides, the edges are flat or wavy teeth and hairy. Flowers bloom in June in pink, running 2 to 4 on the axilla, 7 ~ 9mm in diameter, gathered at the ends of branches, so they look like inflorescences. Petioles are hairy, about 3mm long. There are ten ridges on the calyx. Corolla is reddish purple, 7 ~ 8mm long, with fine hairs and preoccupation on the outside. There are four surgeries. Fruits are small nuts that ripen in dark brown in September. The stem is thicker, about 15mm long, and about 1cm long, and is called island thyme (var. Japonica). It is planted for ornamental purposes because of its fragrance, and it is used for Jinhae, Jinkyung, and Gupung because it has essential oils. Like the thyme, it is distributed in Korea, Japan, China, Mongolia, India, and the like.
The torrent tree (Torreya nucifera) is an evergreen tree of the creeper's conifer tree family, also known as Yasam (野 杉), as a tree specializing in Korea. The fruit is used as a medicine for insect repellent, hair growth, dryness, landscaping, and creation blood, and seeds are used for food by squeezing oil. The leaves have a unique scent, which can be used to burn off branches or fresh leaves to create smoke that prevents mosquitoes from approaching.
Such non-trees are applicable to the preparation of therapeutic or prophylactic extracts, and can be variously applied to pesticides, medicines, cosmetics, household goods, foods, etc., which are commonly used as antimicrobial agents. For example, it can be applied to fungicides, antibacterial and disinfectants in medicine, antibiotics or anti-fouling agents in medicines, preservatives in foods, anti-dandruff or athlete's foot in cosmetics and household goods, anti-armpit extraction, acne. In particular, it can be applied to antiseptic or antibacterial or sterilizing purposes such as cleaning or dish washing detergents, as well as products directly related to microorganisms.
The rapeseed is a representative spring flower coloring yellow places around Jeju Island in mid April. It grows up to 80-130cm in height and has a smooth green surface. Around 15 primary branches come out from the main stem, and 2 to 4 secondary branches come out again from this branch. The leaves are lanceolate and have a dull tip. The leaves on the lower stem have long petioles, and the leaf edges are deeply split. Flowers hang in the end of branch branching inflorescence in March-April. The length of mature ears varies depending on the location of the branches, planting methods, varieties, etc., but it is usually 35-45cm, and 30-40 fruits per ear. Fruit is a convex, cylindrical, about 8cm long.
The main components of the rapeseed, fat, protein, seeds contain 38-45% oil, containing 15-20% soluble nitrogen and 20% protein, the second consumption of edible oil after soybean oil. It contains rutin, erucic acid, campesterol, and brassicasteol. Known pharmacological action is that the pancreas is bleeding due to excessive labor, or when blood is mixed with feces due to dysentery, hemostasis is excellent. It is also used for solo, febrile, swelling and mastitis. In addition, the seed is called a pan head and is used for boils.
The Agatho panax (Acantho panax) is a woody plant of the family Ogapi, that is, called the Ogapi trees, looks and ecology resemble wild ginseng. It is used as a medicine to control strokes and weaknesses in civilian and oriental medicine. Especially, thorny scabies are used to detoxify various chemicals including radioactivity, lower cholesterol in blood, lower blood sugar levels, treat neurological disorders, and improve endurance and concentration. It is known to relieve fatigue of the brain and vitalize all the body's functions.
Ogapi contains acanthosides (Acanthoside B, D) and water-soluble polysaccharides that increase immunity. Chisanoside on the leaves has a pharmacological function, and in the roots there are not only Ogapi glycosides, but also Syringin and coumarin glycosides. Types of Ogapi include Prickly Ogapi, Common Ogapi and Scented Ogapi. Eleutherococcus Senticosus and Acanthop anax senticosus are deciduous broad-leaved shrubs belonging to the elm family, such as ginseng. Use and peel as medicine.
The herbal medicines as described above can be used in the form of an extract prepared by filtration or separation by pulverizing or immersing the powdered state of the mixture by mixing in the same ratio by 5 to 40% by weight in a solvent such as water, alcohol, ethyl ether and the like.
The medicinal herb fermentation extract used in the anti-aging cosmetic composition according to the present invention may be obtained by adding the medicinal herb to the basic medium when culturing yeast, lactic acid bacteria or bacteria, and culturing the fermentation. At this time, the total content of the herbal medicines is preferably included in 1 to 20% by weight based on the basal medium.
Herbal fermentation extract obtained as described above is preferably included in the range of 0.0001 to 100% by weight in the anti-aging cosmetic composition of the present invention. At this time, when the content is less than 0.0001% by weight, it is difficult to expect a substantial aging improvement effect.
The formulation of the anti-aging cosmetic composition containing the herbal fermented extract of the present invention as an active ingredient is not particularly limited and may be appropriately selected as desired. For example, the cosmetic composition of the present invention may be prepared in the form of external skin ointment, softening longevity, nourishing longevity, nutrition cream, massage cream, essence, pack, emulsion, oil gel and the like.
At this time, the external skin ointment may be carried out in the form of 50 to 97% by weight of petrolatum and 0.1 to 5% by weight of polyoxy ethylene oleyl-ether phosphate in addition to the herbal fermentation extract of the present invention. In addition to the fermented extract of the herbal medicine of the present invention, the flexible softener is 1-10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05-2% by weight of surfactant (polyethylene oleyl ether, polyoxyethylene hydrogenated castor oil, etc.) It may also be prepared in a form containing. In addition, nutritional longevity and nourishing cream is 5-20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and wax components (cetanol, stearyl alcohol, beeswax, etc.) in addition to the herbal fermentation extract of the present invention It may be prepared in a form containing more%, the essence may be prepared to further contain 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol in addition to the herbal medicine fermentation extract of the present invention.
Massage cream is prepared by containing 30 to 70% by weight of oil, such as liquid paraffin, petrolatum, isononyl isononanoate in addition to the herbal medicinal fermentation extract of the present invention, the pack is polyvinyl alcohol 5 Produced in a peel off pack containing -20% by weight. In addition to the herbal fermentation extract of the present invention in general emulsified cosmetics can be prepared as a wash off pack containing 5-30% by weight of pigments such as kaolin, talc, zinc oxide, titanium dioxide.
The anti-aging cosmetic composition according to the present invention contains oils, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, or fragrances, etc., which are formulated in general skin cosmetics in addition to the components described above in each formulation. It can also be implemented in the form which mix | blends suitably as needed.
Anti-aging cosmetic composition according to the present invention is excellent in the cell proliferation effect, antioxidant effect, collagen synthesis increase effect, moisturizing effect, as well as the effect of improving the aging of the skin, it can ensure the sustainability of the effect.
In the following, preferred embodiments of the present invention will be described in detail, but the scope of the present invention is not limited only to the following preferred embodiments.
Example 1. Manufacture of Chinese Herb Fermented Extract
First, 100 g mixed with at least one of the dried sheep, thyme, non-tree, rapeseed, and agap in the same amount is sterilized by washing three times with water at 100 ℃ (S10). The sterilized raw material mixture was inoculated with a certain amount of yeast (Saccharomyces cerevisiae 11201) in a predetermined amount (1 × 10 7 per 100 g of raw material mixture) and fermented at 40 ° C. for 48 hours (S20). The fermented raw material was left to dry at 50 ° C. for 3 hours, dried, pulverized to obtain fermented powder. 500 g of distilled water, which was a solvent, was aged at room temperature for 7 days, and then filtered through a 400 mesh filter cloth. Filtration again with a 0.45 μm filter gave 420 g of an extract having a solid content of 1.45 (w / v)%.
Table 1 shows examples obtained by extracting by the same amount and method as in Example 1 with each corresponding extraction solvent.
Here, the herbal fermentation extract may be contained in a range of 0.01 to 10 parts by weight based on 100 parts by weight of each solvent as a dry weight. When the content range of the medicinal herb fermentation extract is less than 0.01 parts by weight or more than 10 parts by weight of each solvent, the anti-aging effect such as antioxidant, cell activation through cell proliferation, collagen synthesis enhancement is more than within the range Problems with falling
And it is also possible to obtain an example obtained by extracting in the same manner as in Example 1 each raw material to the content as shown in Table 2 below. The units of each content here are by weight.
Comparative Example 1
The dried yangja, thyme, non-tree, rape blossoms and the content of Ogapi are mixed as shown in any one of the above Table 2, extracting 500g of distilled water as an extraction solvent by connecting a cooling condenser in a water bath at 80 ℃ 3 hours After cooling for 1 day and then precipitated with a filter of 0.2 ㎛ to prevent the formation, and UV sterilization for 3 days for sterilization effect to obtain the final herbal extract, that is, Comparative Example 1 for comparison with the composition according to the present invention.
Comparative example 2 to 18
In order to measure the activity of the cell proliferation effect, antioxidant effect, collagen synthesis increase effect, moisturizing effect of Examples 2 to 19, a predetermined herbal extract obtained by excluding fermentation is used as Comparative Examples 2 to 18.
Experimental Example 1. Experiments on Cytotoxicity
MTT (3- [4,5-dimethylthiazole-2), a water-soluble and yellow salt, was succinate dehydrogenase (succinatedehydrogenase or mitochondrial dehydrogenase) present in the mitochondria of living cells. The MTT assay is carried out using the principle that -yl] -2,5-diphenyl tetrazolium bromide) is reduced to a water-insoluble blue formazan derivative.
The MTT assay is a test method that is widely used for cell proliferation or toxicity by measuring the number of living cells. Formazan derivatives are dissolved in a solubilizer (usually dimethylsulfoxide) and then measured for absorbance.
More specifically, human fibroblasts cell line (ATCC, CRL-2310) is inoculated in a 24-well plate at a concentration of 1 × 10 4 cells / ml. The medium uses DMEM (Dubelcco's Modified Eagle Medium, BRL, USA) containing 10% bovine serum. 24 hours after inoculation, the cells were replaced with DMEM containing 2% bovine serum, and 10 μl of the test sample was added at a concentration of 50 μg / ml, followed by incubation in a 37 ° C., 5% CO 2 incubator for 3 days. After the incubation, the supernatant was removed, washed again by adding 200 µl of PBS (phosphate buffered saline), 1.0 ml of MTT solution was added per well, and after 4 hours, MTT was removed and DMSO was welled. After 1.0 ml of sugar was added, absorbance was measured at 570 nm after incubation at 37 ° C. overnight. The results are shown in Table 3 below. The cell survival rate (%) is calculated by the following equation (1).
As a result of these experiments, in Examples 1 to 18, as well as almost no cytotoxicity, it can be seen that the cell viability is very excellent.
Experimental Example 2. PGE 2 , IL -6 suppression effect
Human fibroblasts are seeded in 6-well culture plates at a density of 1 × 10 5 cells and incubated in 37 ° C., 5% CO 2 incubator for 24 hours. After treatment with 500 μM of H 2 O 2, the cells were stimulated for 24 hours, the samples were treated by concentration, reacted for 48 hours, and the culture solution was collected and subjected to an ELISA assay. Here, PGE 2 is used as a kit of Assay Design, IL-6 is used as a kit of Endogen (Endogen), and tested according to the manual of each company, the samples are the Examples 1 to 18 and Comparative Example 1 To 18 extracts are used. Inhibitory effect is calculated according to the following equation (2), the results are shown in Table 4.
sample
(Inhibition effect)
(Inhibition effect)
sample
(Inhibition effect)
(Inhibition effect)
(62.7%)
(85.7%)
(37.9%)
(39.0%)
(59.8%)
(85.2%)
(24.3%)
(37.1%)
(66.9%)
(87.9%)
(24.2%)
(32.6%)
(65.3%)
(87.4%)
(30.8%)
(32.3%)
(64.2%)
(86.7%)
(64.2%)
(33.2%)
(71.5%)
(88.9%)
(17.0%)
(35.0%)
(67.5%)
(88.4%)
(19.2%)
(33.9%)
(64.7%)
(87.6%)
(16.7%)
(31.2%)
(56.2%)
(87.6%)
(16.0%)
(33.4%)
(58.7%)
(85.5%)
(19.5%)
(34.7%)
(67.4%)
(89.5%)
(19.4%)
(36.4%)
(69.7%)
(87.7%)
(19.7%)
(35.2%)
(66.4%)
(86.2%)
(13.0%)
(32.4%)
(66.8%)
(86.8%)
(19.4%)
(34.1%)
(67.8%)
(87.6%)
(18.7%)
(32.8%)
(67.4%)
(87.1%)
(18.0%)
(31.6%)
(66.5%)
(84.2%)
(17.4%)
(30.3%)
(64.8%)
(85.3%)
(16.7%)
(29.0%)
As shown in Table 4, the production amount of the inflammatory mediators PGE 2 and IL-6 is significantly reduced than that of the comparative examples 1 to 18 extracts of Examples 1 to 18, showing a high inhibitory effect. Among them, it can be confirmed that the effect of Example 6 extracted with 30% hydrous butylene glycol is the best.
Experimental Example 3. Antioxidant Measurement Experiment
Free radical scavenging activity can be measured to confirm the antioxidant activity of the herbal fermentation extract of each of the Examples 1 to 18.
The test sample uses the herbal fermentation extract of each of Examples 1 to 18, and the free radical scavenging activity is measured by using DPPH. DPPH is purchased from Sigma Co., Ltd, USA.
First, a standard DPPH solution of 400 μm concentration is made. In addition, ethanol was added to the fermented medicinal herb extracts of Examples 1 to 18 and ascorbic acid as an antioxidant, respectively, to prepare a sample at a concentration of 200 μg / ml.
Next, the sample and the standard DPPH solution are added in the same ratio and stirred well, followed by reaction at 37 ° C. for 30 minutes, and the absorbance at 520 nm. At this time, Comparative Examples 1 to 18 are used as a control. Using the following equation (3) to obtain the free radical erasure rate (%), the results are shown in Table 5.
As shown in Table 5, in the case of the extraction solvent, 30% hydrous butylene glycol and propylene glycol applied in Examples 5 and 6 showed high antioxidant effects of 30% or more, respectively, and in the case of composition ratios, Examples 16, 17, and 18 Shows a relatively high antioxidant effect, which corresponds to 30% of each.
Experiment 4. Desiccator Used Moisturizing Measure
In order to determine the moisturizing effect of the herbal medicinal herb extracts of Examples 1 to 18, the moisturizing power can be measured using the difference in the rate of evaporation of water in the liquid as follows. Here, each of Comparative Examples 1 to 18 is used as a control, the specific process is as follows.
After 5 ml of the medicinal herb fermentation extracts of Examples 1 to 18 were titrated in a cup having a constant surface area, completely dried calcium chloride was used as an absorbent, and left for 24 hours in a desiccator adjusted to a temperature of 35 ° C. The moisturizing power is measured as shown in Equation 4 using the average value of 5 times the weight of, and the rate of increase in moisturizing power as shown in Equation 5 below. The results are shown in Table 6.
Where S is the weight of the initial sample and ΔS is the weight of the final sample minus the weight of the final sample.
Moisturizing capacity increase rate (times)
As a result of measuring the moisturizing power by using a desiccator, it was found to be higher in all examples than the control, and in particular, when the 30% hydrous butylene glycol was applied, the moisturizing power increased by 1.61 times. As a result, it can be seen that when the 30% hydrous butylene glycol is applied, the moisture content lasts for the longest and the best moisturizing effect is exerted.
Experiment 5. Determination of Moisturizing Power by Using a Corneometer
The moisturizing effect on the herbal fermented extracts of Examples 1 to 18 may also be measured using a moisturizing power meter (Courage + Khazaka Electronic, CM 825). The control herein also uses Comparative Examples 1 to 18, respectively.
The test method was divided into 8 sections of 2x2 cm 2, upper arm of 8 children (average 7 years old) per each sample, divided into 8 sections, washed several times with water, and then removed moisture to avoid irritation. After 15 minutes, the sample is applied evenly by dropping 10 µl into each of the two sections. After another 2 hours, measure 5 times per one compartment using the moisturizing force meter.
Do this twice a day, twice a week, for four weeks. The experiment is conducted in a constant temperature and humidity room with a room temperature of 21 ± 2 ° C and a relative humidity of 50 ± 5%. The measured value is obtained by averaging the values obtained by measuring five times for 10 subjects, and the numerical values are shown as values of 60 for normal normal skin and 50 to 60 for dry. Before application, it is represented by the value of 55 (average value). Moisturizing power and the amount of increase in moisturizing power are calculated according to the following Equations 6 and 7.
Here, S corresponds to the moisturizing power before applying the sample, ΔS corresponds to the moisturizing power after 2 hours after applying the sample.
As a result of measuring the skin's moisturizing power and the amount of increase using the moisturizing power meter, it can be seen that the moisturizing power after application is increased more than before application. The most increased to 10, the moisturizing effect appears to be the best.
Experiment 6. Collagen Synthesis Effect
The fermentation extract of herbal medicines as in Examples 1 to 18 may be added to the culture solution of human-derived fibroblasts to confirm collagen synthesis promoting effect at a cellular level through a predetermined experiment. Biosynthetic collagen measurements were quantified using the POCP EIA kit (Procollagen Type I C-e Enzyme Immuno Assay KIT). The experimental process is as follows.
Each of the medicinal herb fermentation extracts corresponding to Examples 1 to 18 was produced at a concentration of 2.0% by weight, and added to each culture medium of human-derived fibroblasts, and then cultured for 1 day.
Subsequently, the culture medium is taken and measured at 450 nm using a spectrophotometer to measure the degree of collagen biosynthesis at each concentration with a PECP EIA Kit. In this case, in order to compare the collagen biosynthesis effect, the same procedure is measured in the culture medium and the control group containing no extract to which vitamin C was added.
Collagen biosynthesis was calculated as the relative synthetic ability relative to the control (no addition) and the results are shown in Table 8 below. The experimental value of Table 8 is an average value which was repeated 6 times.
division
% Increase
division
% Increase
Control group (serum-free medium)
100
(Final concentration 51.9 ug / mL)
118.3
As shown in Table 8, the herbal fermented extract included in the anti-aging cosmetic composition according to the present invention can be confirmed that the collagen synthesis enhancement effect at the cellular level.
Formulation Example One.
Formulation examples of astringent cosmetic water in the cosmetic composition containing the herbal medicine fermentation extract of Example 6 described above can be carried out as shown in Table 9. Here, a comparative example is a case where Example 6 is not included.
division
Raw material
(weight%)
(weight%)
Formulation Example 2.
In addition, the formulation examples of the flexible cosmetics in the cosmetics containing the herbal medicine fermentation extract of Example 6 can be carried out as shown in Table 10. At this time, the comparative example is a case where Example 6 is not included.
division
Raw material
(weight%)
(weight%)
Formulation Example 3.
Formulation example of the lotion in the cosmetic composition containing the herbal medicine fermentation extract of Example 6 can be carried out as shown in Table 11. At this time, the comparative example is a case where Example 6 is not included.
(weight%)
(weight%)
Formulation Example 4.
Formulation example of the cream in the cosmetic containing the herbal medicine fermentation extract of Example 6 can be carried out as shown in Table 12. At this time, the comparative example is a case where Example 6 is not included.
(weight%)
(weight%)
Formulation Example 5.
Formulation examples of the essence in the cosmetic containing the herbal medicine fermentation extract of Example 6 can be carried out as shown in Table 13. At this time, the comparative example is a case where Example 6 is not included.
(weight%)
(weight%)
Formulation Example 6.
Formulation examples of the eye cream in the cosmetic containing the herbal medicine fermentation extract of Example 6 may be carried out as shown in Table 14. At this time, the comparative example is a case where Example 6 is not included.
(weight%)
(weight%)
Formulation Example 7.
Formulation examples of the foundation in the cosmetic composition containing the herbal medicine fermentation extract of Example 6 can be carried out as shown in Table 15. At this time, the comparative example is a case where Example 6 is not included.
(weight%)
(weight%)
Moisture Meter corneometer Moisturizing effect
Formulation Example 4, the moisturizing effect when applying the skin of nourishing cream can be confirmed through the following clinical experiments. 20 healthy men, women and children aged 5 or older (age distribution: 5-10 years, average age: 6 years, 7 men, 13 women) who have no history of atopic dermatitis or other eczema. The subject was made.
After measuring the amount of water in the arm of the subject using a coronometer (Corneometer), 20 μl of each sample was applied to the inside of the arm 2 × 2 cm 2. All experiments were performed three times in a sterile chamber with a temperature of 25 ° C. and a humidity of 35.5%, and were measured after 30 minutes, 2 hours, and 4 hours after application of the product. As a control, a cream formulation containing no herbal fermentation extract was used as described above.
As shown in Table 16, it can be seen that the moisturizing effect is much higher in the product containing the herbal medicine fermentation extract according to the present invention.
Measurement of skin elasticity effect
In order to measure the skin elasticity effect on the nutrition cream corresponding to Formulation Example 3 and Comparative Formulation Example 3, the experiment of the following method was conducted in healthy women aged 35 to 50 years.
40 women aged 35 to 50 were divided into two groups of 20 people each, and the first group continued to apply Comparative Formulation Example 3 and the second group to Formulation Example 3 twice a day for 1 month. . Skin elasticity was measured by Cutometer SEM575 and the results are shown in Table 14. Here, in the case of the measured elasticity and moisturizing power, it was expressed as increased elasticity and moisturizing power compared to the initial.
The skin elasticity of the formulation Example 3 containing the herbal fermented extract according to the present invention is compared to Comparative Formulation Example 3, which does not contain the herbal fermented extract of the present invention. You can see that it is three times better.
Skin irritation test result
The test method was a skin patch test on the left upper arm of 30 women aged 35 to 50 years. The patch was used as a finn chamber, and the applied amount of the product was 0.2 ml each. The patch was removed after 48 hours, the skin irritation index was determined according to the criteria of Table 18, and the average skin reactivity was calculated according to each formulation. The results are shown in Table 19.
As shown in Table 19 above, it can be sufficiently guessed how safe the anti-aging cosmetic composition of the present invention in terms of the non-irritating point in the Examples for all formulations.
In the above, a preferred embodiment of the inorganic hydraulic non-abrasive material composition according to the present invention has been described. However, the present invention is not limited thereto, and various modifications can be made within the scope of the claims and the detailed description of the invention. This also belongs to the scope of the present invention.
Figure 1 is a process flow diagram schematically showing the herbal medicine fermentation extraction process of the anti-aging cosmetic composition according to the present invention.
Claims (6)
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101155714B1 (en) * | 2009-11-17 | 2012-06-12 | (주)아모레퍼시픽 | Cosmetic composition containing immature fruit or seed extract of torreya nucifera |
WO2015115687A1 (en) * | 2014-01-29 | 2015-08-06 | 주식회사 유니베라 | Composition for treating and alleviating obesity, containing zingiber moiga leaf extract |
KR102095031B1 (en) * | 2018-11-07 | 2020-03-31 | 주식회사 프롬바이오 | Composition for moisturing, anti-wrinkle or whitening with the extract of Zingiber mioga |
EP4043023A4 (en) * | 2019-09-25 | 2023-11-22 | Murata Manufacturing Co., Ltd. | Collagen production enhancer, pharmaceutical, cosmetic, and method for producing collagen production enhancer |
-
2009
- 2009-09-24 KR KR1020090090344A patent/KR20110032715A/en not_active Application Discontinuation
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101155714B1 (en) * | 2009-11-17 | 2012-06-12 | (주)아모레퍼시픽 | Cosmetic composition containing immature fruit or seed extract of torreya nucifera |
WO2015115687A1 (en) * | 2014-01-29 | 2015-08-06 | 주식회사 유니베라 | Composition for treating and alleviating obesity, containing zingiber moiga leaf extract |
KR102095031B1 (en) * | 2018-11-07 | 2020-03-31 | 주식회사 프롬바이오 | Composition for moisturing, anti-wrinkle or whitening with the extract of Zingiber mioga |
EP4043023A4 (en) * | 2019-09-25 | 2023-11-22 | Murata Manufacturing Co., Ltd. | Collagen production enhancer, pharmaceutical, cosmetic, and method for producing collagen production enhancer |
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