KR20100012361A - Preparation method of coated transglutaminase particles for air-stability - Google Patents

Preparation method of coated transglutaminase particles for air-stability Download PDF

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KR20100012361A
KR20100012361A KR1020080073708A KR20080073708A KR20100012361A KR 20100012361 A KR20100012361 A KR 20100012361A KR 1020080073708 A KR1020080073708 A KR 1020080073708A KR 20080073708 A KR20080073708 A KR 20080073708A KR 20100012361 A KR20100012361 A KR 20100012361A
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oil
transglutaminase
chitin
gum
cellulose
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문장조
조민호
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(주)지알엔지니어링
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII

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Abstract

PURPOSE: A method for preparing stable transglutaminase particle in the air is provided to prevent reaction or extinction of transglutaminase. CONSTITUTION: A method for preparing stable transglutaminase particle in the air comprises: a step of adding powder containing 1.0% or more transglumitanase into process machine; a step of dispersing 1-50% of first binding agent through bottom spray to granulate; a step of dispersing 1-50% of secondary coating agent using fluid layer to coat; and a step of drying a product which is secondarily coat. The transglutaminse particle contains 0.1-10% of transglutaminase and has 0.25-20 mm size.

Description

공기 중 안전성을 위해 피복 된 트랜스글루타미나아제입자의 제조방법.{Preparation method of coated Transglutaminase particles for air-stability}Preparation method of coated Transglutaminase particles for air-stability}

본 발명은 Transglutaminase효소제가 가지는 본래의 기능을 유지 하면서도 공기 중에서 안정하며, 환경적인 요인이 아닌 외부 압력에 의해 coating 물질이 깨지면서 효소제의 기능을 발휘하도록 하는 특성을 지닌 공기 중 안정한 Transglutaminase 효소입자의 제조 방법이다.The present invention is a method for producing a stable transglutaminase enzyme particles in the air having the characteristics of maintaining the original function of the transglutaminase enzyme is stable in the air, the coating material is broken by the external pressure rather than environmental factors to exhibit the function of the enzyme agent. to be.

본 발명은 유동층 공정기를 이용하여 저온에서 Transglutaminase효소를 1.0% 또는 그 이상의 함유하는 파우더를 유동화시켜 Granualating 하고 Coating하여 공기 중 안정한 Transglutaminase 입자를 제조하는 기술이다.The present invention is a technique for producing stable transglutaminase particles in the air by fluidizing and coating powder containing Transglutaminase enzyme 1.0% or more at low temperature using a fluidized bed process.

유동층 공정기는 화학공업에서의 합성, 반응뿐만 아니라 분체의 이송, 혼합, 열교환, 코팅, 건조, 분리, 과립, 흡착 등에 널리 사용되는 핵심공정으로 1926년에 처음으로 산업화에 적용된 분야이며 현재는 제약 및 비료산업에서 Granulating과 coating 목적으로 중요하게 사용되는 설비이다. Fluidized bed process is a core process widely used in chemical industry for synthesis, reaction as well as powder transfer, mixing, heat exchange, coating, drying, separation, granulation, and adsorption. It is an important equipment used for granulating and coating purposes in the fertilizer industry.

Tranaglutaminase효소제는 Streptoverticillium mobaraense의 배양물로 부터 추출한 후 찬 에틸 알콜로 처리하여 얻어진 효소제로 백색∼엷은 황색∼진한 갈색의 분말, 입상, 덩어리 또는 투명∼진한 갈색의 액체로 된 식품제조용 첨가물이다. 물에 용해되고 에탄올에 용해되지 않는다. 방선균에서 추출한 효소의 최적 pH는 6∼7이며, 최적온도는 50∼55℃이다. 역가조정, 품질보존 등을 위하여 희석제, 안정제 등을 첨가할 수 있으며, 흡습성이 강하므로 냉암소에 밀봉 보존하여야 한다. 이러한 Transglutaminase는 유당이나 dextrin 등의 부형제와 혼합되고 밀봉하여 Transglutaminase 1.0% 또는 그 이상의 함유량을 가지는 제품으로 시중에 판매되고 있다. 이러한 Transglutaminase 제품을 기존에 식품첨가물로 주로 사용하였으나 현재는 Transglutaminase의 특성을 이용하여 샴푸나, 에센스, 린스 등의 모발제품에 첨가하여 각종 유효성분이 두피에 밀착되어 그 성분을 지속적으로 발휘 될 수 있도록 가교 역할을 하는데 쓰이게 하는 목적이 있다. 하지만 공기 중에 불안정한 Transglutaminase효소의 특성 때문에 Transglutaminase효소제의 외부를 보호해 줄 수 있는 물질로 피복이 이루어져야 한다.Tranaglutaminase enzyme is an enzyme preparation obtained from the culture of Streptoverticillium mobaraense and treated with cold ethyl alcohol. It is a food additive in white to pale yellow to dark brown powder, granule, lump or transparent to dark brown liquid. Soluble in water and insoluble in ethanol. The optimum pH of the enzyme extracted from actinomycetes is 6-7, the optimum temperature is 50-55 ℃. Diluents, stabilizers, etc. can be added for potency adjustment and quality preservation. The hygroscopicity is strong and must be sealed and stored in a cool dark place. Transglutaminase is commercially available as a product having a content of 1.0% or more of Transglutaminase by mixing and sealing it with an excipient such as lactose or dextrin. Although Transglutaminase products have been mainly used as food additives, they are currently added to hair products such as shampoos, essences and rinses using the properties of Transglutaminase, so that various active ingredients adhere to the scalp so that their components can be continuously exerted. It is intended to be used to play a role. However, due to the nature of unstable transglutaminase enzymes in the air, the coating should be made of a material that can protect the outside of the transglutaminase enzyme.

본 발명은 Transglutaminase효소제 기존 제품의 강한 흡습성과 공기 중 불안정으로 인한 모발제품 적용의 어려움을 해소하여 샴푸 및 에센스등 모발제품 속에서 장기적으로 안정하고 그 성능이 뛰어난 Transglutaminase 입자의 제조방법에 관 한 것으로써 1차적으로 Transglutaminase효소제 분말의 입자 화 과정을 거친 후 2차로 공기 중 산소와 반응하지 않는 2차 피복물질을 사용하여 바텀 스프레이(bottom spray)방식으로 Coating하여 안정한 Transglutaminase 입자를 제조한다.The present invention solves the difficulty of applying hair products due to the strong hygroscopicity and instability of the existing products of Transglutaminase enzyme, and relates to a method for producing long-term stable and excellent Transglutaminase particles in hair products such as shampoo and essence. First, after the granulation process of Transglutaminase enzyme powder, the second coating material that does not react with oxygen in the air is coated by bottom spray method to produce stable Transglutaminase particles.

Transglutaminase 의 coating을 통하여 샴푸나, 린스, 에센스, 헤어 팩 등 모발제품 속에서 장기적으로 안정하게 효소의 기능이 유지되고, 사용자가 사용 할 경우 피막물질이 깨져 내부의 Transglutaminase효소가 그 기능을 발휘하게 되면서 동시에 모발제품 내의 유효성분을 두피에 장기간에 걸쳐 결착시켜 샴푸 및 각종 미용제품의 성능을 지속적이고 보장 한다.Through the coating of Transglutaminase, enzyme function is maintained in long-term stable condition in hair products such as shampoo, rinse, essence, hair pack, and when the user uses it, the coating material is broken and the internal Transglutaminase enzyme exerts its function. At the same time, the active ingredients in the hair products are bound to the scalp for a long time to ensure the performance of shampoos and various beauty products.

본 발명의 제조방법을 공정별로 구분하면 다음과 같다.The production method of the present invention is classified as follows.

제 1 공정 (Transglutaminase의 granualating)First Process (Granualating of Transglutaminase)

본 발명에서는 시중에 판매되는 1.0%의 Transglutaminase를 포함하는 파우더를 유동층 공정기에 투입하고 Inlet Temp 40~80℃에서 바텀 스프레이(bottom spray)형태로 Transglutaminase파우더 중량 비 1~30%의 전분을 10 Brix 농도로 바텀스프레이하여 0.2~1.0mm의 입자를 만든다. In the present invention, a powder containing 1.0% of Transglutaminase, which is commercially available, is introduced into a fluidized bed process machine, and the starch of 1-30% by weight of Transglutaminase powder is in the form of a bottom spray at Inlet Temp 40 to 80 ° C. Bottom spray with particles to produce particles of 0.2-1.0 mm.

제 2 공정 (Transglutaminase 의 coating)Second Process (Transglutaminase Coating)

Inlet Temp 40~80℃에서 바텀 스프레이(bottom spray)형태로 제 1공정에 만들어진 Transglutaminase입자에 중량 비 5~50%의 팜 오일(palm oil)을 coating 한다.Inlet Temp At 40 ~ 80 ℃, palm oil of 5 ~ 50% by weight is coated on Transglutaminase particles made in the first process in the form of bottom spray.

[실험예 - 45℃에서 1주 보관된 제품 중 효소의 잔류 활성 측정 ]Experimental Example-Measurement of Residual Activity of Enzyme in a Product Stored at 45 ° C for One Week

상기 실시 예에서 제조된 트랜스 글루타미나아제 입자에 대한 안정성을 측정하였으며 실험 결과는 다음과 같다.Stability of the transglutaminase particles prepared in the above example was measured and the experimental results are as follows.

Figure 112008054365791-PAT00001
Figure 112008054365791-PAT00001

도 1: 피복되어 공기 중 안정한 Transglutaminase 입자(0.2mm~1.5mm).1: Transglutaminase particles coated (0.2 mm-1.5 mm) which are stable in air.

도 2: 일반적으로 판매되는 Transglutaminase효소 함유량이 1.0%인 파우더(20μm~200μm).2: Powder (20 μm to 200 μm) of 1.0% Transglutaminase enzyme content commonly sold.

Claims (3)

transglutaminase효소를 1.0% 또는 그 이상 함유하는 파우더를 유동층 공정기에 투입하여 공기의 흐름에 따라 유동화 시키고 투입된 파우더 중량 비 1~50%의 1차 결착제를 바텀 스프레이(Bottom Spray)방식으로 분산시켜 과립화 한다. 1차 공정으로 과립 된 입자의 중량 비 1~50%의 2차 코팅제를 연속적으로 유동층 공정기를 이용해 바텀 스프레이(Bottomspray)방식으로 분산시켜 coating한다. 2차 coating까지 마친 제품을 건조 시키고 사이즈가 0.5~2.0mm 이며, transglutaminase효소를 0.1~10%를 포함하는 공기 중 안정한 transglutaminase 입자를 제조하는 방법.Powder containing 1.0% or more of transglutaminase enzyme is introduced into a fluidized bed process to fluidize according to the flow of air, and granulated by dispersing the primary binder of 1-50% by weight of the injected powder by the bottom spray method. do. The secondary coating agent with a weight ratio of 1 to 50% of the granulated particles by the first process is continuously coated by a bottom spray method using a fluidized bed process machine. Drying the finished product up to the second coating, the size is 0.5 ~ 2.0mm, the method for producing a stable transglutaminase particles in air containing 0.1 ~ 10% transglutaminase enzyme. 상기 제 1항에 있어서 상기 1차 결착제로서는 아크릴레이트 공중합 변성체, 아크릴산 소다, 아크릴계폴리머, 스티렌 공중합체, 폴리비닐 유도체, 메타크릴산, 아크릴산에틸공중합체류, 유드래지트 그리고 전분(Starch), 덱스트린(Dextrin), 제인(Zein) 등의 옥수수 단백추출물 및 이를 인위적으로 가공한 유사물질, 알긴산나트륨, 알긴산, 쉘락류, 카복시비닐폴리머, 하이드록시프로필메틸셀룰로즈프탈레이트류, 하이드록시프로필메틸셀룰로즈 아세테이트숙시네이트, 하이드록시프로필메틸 아세테이트숙시네이트, 카복시메틸셀룰로즈, 셀룰로즈아세테이트프탈레이트류, 하이드록시프로필셀룰로즈류, 에틸셀룰로즈류, 메틸셀룰로즈류, 폴리비닐아세테이트프탈레이트, 대두단백, 소맥단백, 대두단백 또는 소맥단백의 가공물질, 키틴, 키틴산, 키틴 또는 키틴산의 가공물질, 한천, 카라기난(Carrageenan), 펙틴(Pectin), 구아검(Guar gum), 로우커스트 빈 검(Locust bean gum), 크산탄 검(Xanthan gum), HPMC(hydroxypropyl methylcellulose), 젤라틴(gelatin), 젤란 검(Gellan gum), 아라비아 검(Arabic gum), 콜리코트(Kollicoat), 탄소수 6내지 12의 중쇄 지방산류 중 선택된 어느 하나 또는 둘 이상을 혼합하여 사용되는 바인더.The method of claim 1, wherein the primary binder is an acrylate copolymer modified product, an acrylic acid soda, an acrylic polymer, a styrene copolymer, a polyvinyl derivative, methacrylic acid, an ethyl acrylate copolymer, an eulogy and a starch, Corn protein extracts such as dextrin and zein, and artificially processed analogues thereof, sodium alginate, alginic acid, shellac, carboxyvinyl polymer, hydroxypropylmethylcellulose phthalate, hydroxypropylmethylcellulose acetate succinate Nate, hydroxypropylmethyl acetate succinate, carboxymethyl cellulose, cellulose acetate phthalates, hydroxypropyl cellulose, ethyl cellulose, methyl cellulose, polyvinylacetate phthalate, soy protein, wheat protein, soy protein or wheat protein Processing material, chitin, chitin, chitin or chitin Processed materials, agar, carrageenan, pectin, guar gum, locust bean gum, xanthan gum, hydroxypropyl methylcellulose (HPMC), gelatin (gelatin) ), A gellan gum (Gellan gum), Arabian gum (Arabic gum), Kollicoat (Kollicoat), a binder used by mixing any one or two or more selected from heavy chain fatty acids having 6 to 12 carbon atoms. 상기 제 1 항에 상기 2차 코팅제로서는 쇠기름(beef tallow), 버터기름(butter fat), 파마자유(castor oil), 코코넛 야자유(coconut oil), 옥수수유(corn oil), 면실유(cotton oil), 아마인유(linseed oil), 올리브유(olive oil), 동유(tung oil), 팜 야자유(palm oil), 땅콩유(peanuit oil), 참기름(sesame oil), 대두유(soy oil), 평지씨기름(rapeseed oil), 해바라기기름(sunflower oil), 돼지기름(lard oil), 간유(cod liver oil), 고래기름, 아크릴레이트 공중합 변성체, 아크릴산 소다, 아크릴계폴리머, 스티렌 공중합체, 폴리비닐 유도체, 메타크릴산, 아크릴산에틸공중합체류, 유드래지트 그리고 전분(Starch), 덱스트린(Dextrin), 제인(Zein) 등의 옥수수 단백추출물 및 이를 인위적으로 가공한 유사물질, 알긴산나트륨, 알긴산, 쉘락류, 카복시비닐폴리머, 하이드록시프로필메틸셀룰로즈프탈레이트류, 하이드록시프로필메틸셀룰로즈 아세테이트숙시네이트, 하이드록시프로필메틸 아세테이트숙시네이트, 카복시메틸셀룰로즈, 셀룰로즈아세테이트프탈레이트류, 하이드록시프로필셀룰로즈류, 에틸셀룰로즈류, 메틸셀룰로즈류, 폴리비닐아세테이트프탈레이트, 대두단백, 소맥단백, 대두단백 또는 소맥단백의 가공물질, 키틴, 키틴산, 키틴 또는 키틴산의 가공물질, 한천, 카라기난(Carrageenan), 펙틴(Pectin), 구아검(Guar gum), 로우커스트 빈 검(Locust bean gum), 크산탄 검(Xanthan gum), HPMC(hydroxypropyl methylcellulose), 젤라틴(gelatin), 젤란 검(Gellan gum), 아라비아 검(Arabic gum), 콜리코트(Kollicoat), 탄소수 6내지 12의 중쇄 지방산류 중 선택된 어느 하나 또는 둘 이상을 혼합하여 사용되는 바인더.According to claim 1, wherein the secondary coating agent is beet tallow, butter fat, butter oil (castor oil), coconut palm oil (coconut oil), corn oil (cotton oil), cotton oil (cotton oil), Linseed oil, olive oil, tung oil, palm palm oil, peanut oil, sesame oil, soy oil, rapeseed oil oil, sunflower oil, lard oil, cod liver oil, whale oil, acrylate copolymer modified product, soda acrylate, acrylic polymer, styrene copolymer, polyvinyl derivative, methacrylic acid , Ethyl acrylate copolymers, Eudrats, and corn protein extracts such as starch, dextrin, and zein, and artificially processed analogs thereof, sodium alginate, alginic acid, shellac, carboxyvinyl polymer, Hydroxypropylmethylcellulose phthalates, hydroxypropylmeth Tylcellulose acetate succinate, hydroxypropylmethyl acetate succinate, carboxymethyl cellulose, cellulose acetate phthalates, hydroxypropyl cellulose, ethyl cellulose, methyl cellulose, polyvinylacetate phthalate, soy protein, wheat protein, soy protein Or the processing material of wheat protein, chitin, chitin, chitin or chitin, agar, carrageenan, pectin, guar gum, low cust bean gum, Xanthan gum, hydroxypropyl methylcellulose (HPMC), gelatin, gellan gum, Arabic gum, Kollicoat, any one of 6 to 12 carbon chain fatty acids Or a binder used by mixing two or more.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016006844A1 (en) * 2014-07-07 2016-01-14 유한책임회사 암브로티아 Wet granulated cell culture medium and preparation method therefor
WO2023076541A3 (en) * 2021-10-29 2023-06-29 Corn Products Development, Inc. Method for obtaining a gelled plant protein composition from sheared plant protein using transglutaminase

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016006844A1 (en) * 2014-07-07 2016-01-14 유한책임회사 암브로티아 Wet granulated cell culture medium and preparation method therefor
EP3168294B1 (en) * 2014-07-07 2020-10-14 Ambrothia LLC Wet granulated cell culture medium and preparation method therefor
WO2023076541A3 (en) * 2021-10-29 2023-06-29 Corn Products Development, Inc. Method for obtaining a gelled plant protein composition from sheared plant protein using transglutaminase

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