KR20060001369A - Preparation method for brand meat and identification method same - Google Patents

Preparation method for brand meat and identification method same Download PDF

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KR20060001369A
KR20060001369A KR1020040050473A KR20040050473A KR20060001369A KR 20060001369 A KR20060001369 A KR 20060001369A KR 1020040050473 A KR1020040050473 A KR 1020040050473A KR 20040050473 A KR20040050473 A KR 20040050473A KR 20060001369 A KR20060001369 A KR 20060001369A
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최창본
백경훈
이명훈
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학교법인 영남학원
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G01N33/12—Meat; Fish
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
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    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated

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Abstract

본 발명은 브랜드 쇠고기 제조방법 및 이의 식별방법에 관한 것이다. 특히 본 발명은 소에서 발현되지 않는 단백질 또는 펩타이드를 항원으로 사용하여 특정 품종의 소를 면역화시키는 것을 포함하는 상기 항원에 대한 항체로 표지된 브랜드 소 및 쇠고기 제조방법을 제공하며, 또한 특정 소 또는 쇠고기내 상기 항체의 존재여부를 확인하여 소 또는 쇠고기의 품종 및 품질을 식별하는 방법을 제공한다. The present invention relates to a method for producing brand beef and its identification method. In particular, the present invention provides a method for producing branded cows and beef labeled with antibodies to the antigens, comprising immunizing certain breeds of cows by using proteins or peptides not expressed in the cows as antigens. It provides a method for identifying the breed and quality of cattle or beef by checking the presence of the antibody.

소, 브랜드육, 식별Cow, brand meat, identification

Description

브랜드 쇠고기 제조방법 및 식별방법{PREPARATION METHOD FOR BRAND MEAT AND IDENTIFICATION METHOD SAME}Brand beef manufacturing method and identification method {PREPARATION METHOD FOR BRAND MEAT AND IDENTIFICATION METHOD SAME}

도 1은 면양의 비면역된 혈청, 면역된 혈청 및 육즙 내 항체 농도를 측정한 것이다.Figure 1 measures the antibody concentrations in sheep's non-immunized serum, immunized serum and gravy.

도 2는 면양의 비면역된 혈청 및 면역된 면양의 육즙에서의 항체 존재여부를 웨스턴 블롯으로 확인한 것이다.FIG. 2 shows Western blots for the presence of antibodies in sheep's non-immunized serum and immunized sheep's juice.

도 3은 한우의 면역된 혈청 및 비면역된 혈청내의 항체 검출결과를 나타낸 것이다.Figure 3 shows the results of antibody detection in the immunized and non-immunized serum of Hanwoo.

도 4는 브랜드 쇠고기 식별용 키트를 간략하게 도시한 것이다.4 is a simplified illustration of a brand beef identification kit.

도 5는 도 4의 키트를 이용한 브랜드 쇠고기 식별원리를 도시한 것이다. 5 illustrates the principle of brand beef identification using the kit of FIG. 4.

도 6은 도 4의 키트를 이용한 경우, 양성 및 음성 결과를 나타낸 것이다.6 shows positive and negative results when the kit of FIG. 4 is used.

[발명이 속하는 기술분야][TECHNICAL FIELD OF THE INVENTION]

본 발명은 브랜드 쇠고기 제조방법 및 식별방법에 관한 것으로, 보다 상세하게는 소에서 발현되지 않는 항원으로 소를 면역화시키므로써, 상기 항원에 대한 항 체로 표지된 브랜드 소의 제조방법 및 상기 항체를 이용한 브랜드 소의 식별방법에 관한 것이다. The present invention relates to a method for producing and identifying a brand beef, and more particularly, by immunizing a cow with an antigen that is not expressed in the cow, thereby producing a brand cow labeled with an antibody to the antigen and a brand cow using the antibody. It relates to an identification method.

[종래기술][Private Technology]

1993년 말 UR(Uruguay Round of Multinational Trade Negotiation)협상이 타결된 이후, 우리나라 한우 사육 두수는 1997년 2,9727 천두에서 쇠고기 수입이 완전 자유화된 2001년에 1,476 천두로 절반 가까운 감소 추세를 나타내었으며, 한우 쇠고기의 시장 점유율 또한 점차적으로 감소하는 추세에 있다. Since the conclusion of the Uruguay Round of Multinational Trade Negotiation (UR) negotiations at the end of 1993, the number of Korean cattle raised in Korea has declined by nearly half, from 2,9727 heads in 1997 to 1,476 heads in 2001. Hanwoo beef's market share is also gradually decreasing.

시장개방 체제는 국가 경쟁력을 갖춘 고품질 한우 쇠고기의 생산을 요구하게 되었으며, 이에 한우의 고급육 생산을 위한 연구(거세를 통한 육질개선, 사료급여 개선, 사료종류 다양화, 사양기간 장기화 등)들이 꾸준히 진행되어져 오고 있다. The market-opening system required the production of high-quality Korean beef, which has a national competitiveness, and therefore, researches for the production of high-quality beef of Hanwoo (steady meat quality through castration, improvement of feed benefits, diversification of feed types, and prolonged feeding period) It has been.

한편, 값싼 수입육이 한우육으로 둔갑되어 판매되는 경우가 발생되면서, 농림부는 2002년 1월부터 축산물 가공처리법 시행규칙을 개정, 육류 판매업자는 거래 내역서에 고기의 종류와 물량, 원산지, 매입처 등 거래내역을 기록하고 이를 1년간 보관해야 하는 이른바 식육거래기록의무제(거래내역 비치제)를 실시하고 있으나, 원산지 허위표시 및 미표시 등 위반사례가 매년 증가하고 있는 실정이다. 따라서, 간편한 조작만으로 현장에서 누구라도 쉽게 한우육과 수입육을 식별할 수 있는 기술의 개발이 요구되고 있다. On the other hand, when cheap imported meat was sold as beef cattle, the Ministry of Agriculture and Forestry revised the Enforcement Regulations of the Livestock Processing Act from January 2002, and meat sellers recorded the transaction details such as meat type, quantity, origin, and place of purchase in the transaction details. There is a so-called meat transaction record obligation (the transaction history system), which must be recorded and kept for one year, but the number of violations such as false and unmarked origin is increasing every year. Therefore, there is a demand for the development of a technology that allows anyone to easily identify Korean beef and imported meat by simple operation.

현재까지 알려진 육류의 식별기법들은 대부분 DNA의 다형성에 기초를 두고 있다. Wyman 등(Wyman A. R. and R. White. 1980. Proc. Natl. Acad. sci. 77:6754~6758)에 의해 최초로 RFLP(Restriction Fragment Length Polymorphisms)기 법이 보고 된 이후, 축종 특이적 DNA 프로브에 관한 연구(Chikuni, K., et al.,. 1990. Meat Sci. 27:119~128.)를 위시해, RAPD(Random Amplified Polymorphic DNA), AFLP(Amplified Fragment Length Polymorphism), MS(Microsatellite), SSCP(Single Strand Conformation Polymorphism), DAF(DNA Amplification Fingerprinting) 등과 같이 분자유전학 분야의 지속적인 발전을 통한 보다 정확하고 신뢰수준이 높은 분석기법들이 개발되어져왔다(Kirby, L. T. 1992. DNA Fingerprinting - An introduction. W. H. Freeman and Company, New York).The meat identification techniques known to date are mostly based on DNA polymorphism. Since the first Restriction Fragment Length Polymorphisms (RFLP) technique was reported by Wyman et al. (Wyman AR and R. White. 1980. Proc. Natl. Acad. Sci. 77: 6754-6758), Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Microsatellite (MS), SSCP, including studies (Chikuni, K., et al., 1990. Meat Sci. 27: 119-128.) More accurate and reliable analytical methods have been developed through continuous developments in the field of molecular genetics such as Single Strand Conformation Polymorphism (DNA) and DNA Amplification Fingerprinting (DAF) (Kirby, LT 1992. DNA Fingerprinting-An introduction.WH Freeman and Company, New York.

특히 Jeffreys 등(Jeffreys, A. J. et al., 1985. Nature. 314:67~73.)에 의해 개발된 DNA 핑거프린팅(fingerprinting)의 경우, 생물체의 유전체상에 나타나는 유전적 변이의 검출과 생물체간의 유전자 구조의 차이(다형성, polymorphism)에 대한 분석이 효율적이라는 장점이 있으며(Kuhnlein, U., et al., 1989. Theor. Appl. Genet. 77:669; Kikkawa, Y., et al., 1995. Biochem. Genet. 33:51.; Pitra, C., et al., 1997. Anim. Reserch. 45:28~36), AFLP기법 또한 실험결과에 대한 재현성과 신뢰도가 매우 높고 유전적 유사도가 가까운 종이나 품종 간에도 고도의 유전적 변이와 다형성을 나타내기 때문에, 종 또는 품종 특이적인 DNA 마커의 검출에 매우 효과적인 것으로 알려져 있다(Otsen, M., et al., 1996. Genomics. 37(3):289~294.). Especially in the case of DNA fingerprinting developed by Jeffreys et al. (Jeffreys, AJ et al., 1985. Nature. 314: 67-73.), The detection of genetic variation in the genome of an organism and the gene between organisms The advantage of efficient analysis of structural differences (polymorphism) is known (Kuhnlein, U., et al., 1989. Theor. Appl. Genet . 77: 669; Kikkawa, Y., et al., 1995. Biochem.Genet . 33:51 . ; Pitra, C., et al., 1997.Anim.Reserch . 45: 28-36), AFLP techniques also have very high reproducibility and reliability of experimental results and close genetic similarities. Because of their high genetic variation and polymorphism, they are known to be very effective for the detection of species- or variety-specific DNA markers (Otsen, M., et al., 1996. Genomics. 37 (3): 289) . ~ 294.).

우리나라의 경우, RAPD기법을 이용한 한우와 젖소(Holstein)의 판별 신뢰도는 거의 정확하다고 알려져 있으며(김태헌. 2001. 유전자 감식법 개발 및 활용. 월간한우 10:98~112), 돼지의 경우 마이크로세텔리트 마커(microsatellite marker)를 이용하여 재래돼지와 랜드레이스를 식별할 수 있는 유전자적 마커가 개발되어있다(김철욱 외, 2001. Korean J. Anim. Sci. 43(6):789~802). 그러나, 하지만 이러한 기법들은 품종에 따라 차이가 나타나며, 면역학적 기법과 비교해 분석에 소요되는 시간이 길며, 고가의 장비 및 분석에 필요한 전문 인력이 요구된다는 단점이 있다.In Korea, the reliability of discrimination between Korean cattle and cows using RAPD is known to be almost accurate (Kim Tae-heon, 2001. Development and application of genetic identification methods. Monthly Korean cattle 10: 98 ~ 112). Genetic markers have been developed to identify conventional pigs and land races using microsatellite markers (Chul-Wook Kim et al., 2001. Korean J. Anim. Sci. 43 (6): 789-802). However, these techniques differ in their varieties, and are disadvantageous in that the analysis time is longer compared to immunological techniques, and expensive equipment and specialized personnel are required.

한편 면역학적 기법은, 항원 또는 항체의 존재를 효소 활성을 이용하여 검출하는 방법으로, 방사성표지물 대신 효소를 사용하므로 안전하다. 특히, ELISA와 같은 진단 기법들은 여타 다른 기법들과 비교하여 보다 편리하고, 신속한 진단이 가능하기 때문에(Demeulemester, C., et al., 1991. J. Sci. Food. Agric. 56:325~333.; Hsieh, Y. H. P., Johnson, M. A., Wetzstein, C. J. and Green, N. R. 1996. J. Food. Qual. 19:1~13.; Janssen, F. W., Voortman, G. and Faaij, J. A. 1987. J. Agric. Food. Chem. 35:563~567.; Samarajeewa, U., Wei, C. I., Huang, T. S. and Marshall, M. R. 1991. Crit. Rev. Food. Sci. Nutr. 29:403~434), 진단 키트 제작 특히, 질병의 조기 진단관련 키트 개발에 가장 많이 활용되어지고 있다(Dong, L. V., et al., 2003. J. Immunol. Methods. 9439:1~19; Langedijk, J. P. M., et al., 2001. J. Clin. Microbiol. 39(3):906~912; Soutullo, A., et al., 2001. Vet. Microbiol. 79:111~121.; Drew, T. W., et al., 1999. Vet. Microbiol. 64:145~154; Eisler, M. C., et al., 1998. Vet. Parasitol. 79:187~201; Cheng, C., et al., 1996. Clin. Diagn. Virol. 6:137~145; Saravi, M. A., et al., 1995. Vet. Immunol. Immunopathol. 47:93~99). On the other hand, immunological techniques are a method of detecting the presence of an antigen or an antibody using enzymatic activity, and are safe because enzymes are used instead of radiolabels. In particular, diagnostic techniques such as ELISA are more convenient and faster to diagnose than other techniques (Demeulemester, C., et al., 1991. J. Sci. Food.Agric. 56: 325-333 Hsieh, YHP, Johnson, MA, Wetzstein, CJ and Green, NR 1996. J. Food.Qual . 19: 1-13 .; Janssen, FW, Voortman, G. and Faaij, JA 1987. J. Agric. Chem. 35: 563 ~ 567; Samarajeewa, U., Wei, CI, Huang, TS and Marshall, MR 1991. Crit. Rev. Food.Sci.Nutr. 29: 403 ~ 434) And early development kits for early diagnosis of disease (Dong, LV, et al., 2003. J. Immunol. Methods. 9439: 1 ~ 19; Langedijk, JPM, et al., 2001. J. Clin.Microbiol . 39 (3): 906-912; Soutullo, A., et al., 2001. Vet. Microbiol. 79: 111-121 . ; Drew, TW, et al., 1999. Vet. Microbiol. 64 : 145-154; Eisler, MC, et al., 1998. Vet. Parasitol. 79: 187-201 ; Cheng, C., et al., 1996. Clin. Diagn. Virol. 6: 137-145; Saravi, MA, et al., 1995. Vet. Immunol. Immunopathol. 47: 93-99 ).

상기한 방법외에도, 품종별 또는 개체별로 존재하는 특이 단백질을 이용한 단백질 다형현상 기법이 있으나, 이는 동일한 축종 내에서도 발현되는 단백질의 변이가 심하여 재현성이 상대적으로 낮은 것으로 알려져 있다.In addition to the above-described method, there is a protein polymorphism technique using a specific protein present in each breed or individual, but this is known to be relatively low in reproducibility due to the severe variation of the protein expressed in the same livestock.

따라서, 쇠고기의 품종 및 품질을 신속, 정확하게 식별할 수 있으며, 전문적인 지식없이도 쉽게 실시할 수 있는 방법의 개발이 요구된다. Therefore, the development of a method that can quickly and accurately identify the breed and quality of beef, and can be easily carried out without expert knowledge.

상기 종래기술의 문제점을 해결하기 위하여 안출된 것으로서, 본 발명은 소에서 발현되지 않는 항원으로 소를 면역화시키므로써 상기 항원에 대한 항체로 표지되는 브랜드 소 또는 브랜드 쇠고기 제조방법을 제공하는 것을 목적으로 한다.In order to solve the problems of the prior art, an object of the present invention is to provide a method for producing a brand cow or brand beef that is labeled with an antibody to the antigen by immunizing the cow with an antigen that is not expressed in the cow. .

또한 본 발명은 면역학적 기법을 이용하여 현장에서 특정 브랜드 육과 여타 다른 품종의 쇠고기를 신속, 정확하게 식별할 수 있는 방법을 제공하는 것을 목적으로 한다.It is also an object of the present invention to provide a method for quickly and accurately identifying specific branded meat and other types of beef in the field using immunological techniques.

또한 본 발명은 브랜드 소 제조를 위한 항원을 제공하는 것을 목적으로 한다.It is another object of the present invention to provide an antigen for producing brand cows.

상기 목적을 달성하기 위하여 본 발명은 (a) 소에서 발현되는 단백질 서열과, 양서류, 곤충, 파충류, 어류 및 식물류로 이루어진 군으로 선택되는 생물에서 발현되는 단백질 서열간의 상동성을 분석하는 단계, (b) 상기 분석결과로부터, 상기 생물에서만 특이적으로 발현되는 단백질 서열을 선별하는 단계, (c) 상기 선별한 단백질 서열에서 항원성 부위를 검색하고, 상기 항원성 부위를 포함하는 펩타이 드를 제작하는 단계 및 (d) 상기 펩타이드를 소에 주입하여 면역화시키는 단계를 포함하는 브랜드 소의 제조방법을 제공한다.In order to achieve the above object, the present invention comprises the steps of (a) analyzing the homology between the protein sequence expressed in cattle and the protein sequence expressed in an organism selected from the group consisting of amphibians, insects, reptiles, fish and plants, ( b) selecting a protein sequence specifically expressed in the organism from the analysis result, (c) searching for an antigenic site in the selected protein sequence, and preparing a peptide comprising the antigenic site And (d) injecting the peptide into the cow to immunize the cow.

또한 본 발명은 (a) 소에서 발현되는 단백질 서열과, 양서류, 곤충, 파충류, 어류 및 식물류로 이루어진 군으로 선택되는 생물에서 발현되는 단백질 서열간의 상동성을 분석하는 단계, (b) 상기 분석결과로부터, 상기 생물에서만 특이적으로 발현되는 단백질 서열을 선별하는 단계, (c) 상기 선별한 단백질 서열에서 항원성 부위를 검색하고, 상기 항원성 부위를 포함하는 펩타이드를 제작하는 단계, (d) 상기 펩타이드를 소에 주입하여 면역화시키는 단계, (e) 상기 소 또는 이의 일부로부터 육즙 또는 혈청을 수득한 후, 이를 상기 (d) 단계의 펩타이드와 반응시켜 면역반응이 형성됨을 확인하는 단계를 포함하는 브랜드 쇠고기의 식별방법을 제공한다.In another aspect, the present invention (a) analyzing the homology between the protein sequence expressed in cattle and the protein sequence expressed in an organism selected from the group consisting of amphibians, insects, reptiles, fish and plants, (b) the analysis result Selecting a protein sequence that is specifically expressed only in the organism, (c) searching for an antigenic site in the selected protein sequence, and preparing a peptide comprising the antigenic site, (d) Injecting a peptide into a cow to immunize, (e) obtaining juice or serum from the cow or a portion thereof, and then reacting it with the peptide of step (d) to confirm that an immune response is formed. Provides a way to identify beef.

이하 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.

본 발명은 소에서 발현되지 않는 단백질 또는 펩타이드에 대하여 면역화되어, 상기 항원(단백질 또는 펩타이드)에 대한 항체로 표지된 브랜드 소의 제조방법 및 상기 항체를 이용한 브랜드 소의 식별방법에 관한 것이다.The present invention relates to a method for producing a brand cow labeled with an antibody directed against an antigen (protein or peptide) that is immunized against a protein or peptide that is not expressed in a cow, and a method for identifying a brand cow using the antibody.

본 발명의 브랜드 소 또는 브랜드 쇠고기는, 검출가능하거나 식별가능한 표지물질로 표지된 소 또는 쇠고기를 의미한다. 상기 검출 또는 식별은, 면역화학방법일 수 있으며, 상기 표지물질은 항체일 수 있다.Brand cow or brand beef of the present invention means cow or beef labeled with a detectable or identifiable labeling substance. The detection or identification may be an immunochemical method, and the label may be an antibody.

본 발명에서는, 소 또는 쇠고기를 표지하는 표지물질을 제공하며, 상기 표지물질은 예컨대 소에서는 발현되지 않는 단백질, 펩타이드 또는 이들의 단편에 대한 항체일 수 있다. 상기 항체는 일차항체 또는 이차항체일 수 있다. 상기 소에서 발현되지 않는 단백질, 펩타이드 또는 이들의 단편은 항원으로 사용하여, 소에 투여하므로써, 표지물질인 항체를 함유하는 소 또는 쇠고기를 제조할 수 있다.In the present invention, a label for labeling a cow or beef is provided, and the label may be an antibody against a protein, peptide or fragment thereof that is not expressed in, for example, a cow. The antibody may be a primary antibody or a secondary antibody. Proteins, peptides or fragments thereof that are not expressed in cows can be used as antigens and administered to cows to produce cows or beef containing antibodies as markers.

본 발명에 따른 항원은, 소에서 발현되지 않는 단백질, 펩타이드 및 이들의 단편으로 이루어진 군으로부터 선택될 수 있으며, 바람직하기로는 양서류, 곤충, 파충류, 어류 및 식물류로 이루어진 군으로 선택되는 생물에서 발현되는 단백질, 펩타이드 또는 이들의 단편으로부터 선택될 수 있으며, 더욱 바람직하기로는 상기 생물은 개구리, 바퀴벌레, 초파리, 애기장대(Arabidopsis) 및 영원(Triturus)으로 이루어진 군으로부터 선택될 수 있다. 본 발명의 항원은 소에서 발현되는 단백질 서열과 상동성이 없는 것일 수 있으며, 바람직하기로는 항원성 부위를 포함한 것일 수 있으며, 더욱 바람직하기로는 서열번호 1 내지 344로 기재한 군으로부터 선택될 수 있다. 또한 본 발명의 항원은, 항체 형성을 용이하게 하기 위하여, 항원의 N-말단 또는 C-말단에 통상의 운반체 단백질, 예컨대, 오발부민 또는 KLH(Keyhole Limpet Hemacyanin)이 더욱 결합된 것일 수 있다. The antigen according to the present invention may be selected from the group consisting of proteins, peptides and fragments thereof which are not expressed in bovine, preferably expressed in organisms selected from the group consisting of amphibians, insects, reptiles, fish and plants. Proteins, peptides or fragments thereof, and more preferably the organism may be selected from the group consisting of frogs, cockroaches, fruit flies, Arabidopsis and Tritus. The antigen of the present invention may be one having no homology with the protein sequence expressed in bovine, preferably may include an antigenic site, more preferably may be selected from the group set forth in SEQ ID NOs: 1 to 344 . In addition, the antigen of the present invention, in order to facilitate the formation of the antibody, a common carrier protein, such as Ovalbumin or KLH (Keyhole Limpet Hemacyanin) may be further bound to the N-terminal or C-terminal of the antigen.

단백질 서열간의 상동성 분석은 공지의 분석프로그램으로 실시할 수 있으며, 항원성 부위는 AntheProt 또는 SciProtein 프로그램을 통하여 결정할 수 있다. Homologous analysis between protein sequences can be performed by known assay programs, and antigenic sites can be determined via the AntheProt or SciProtein program.

본 발명의 브랜드 소는 통상의 면역화방법으로 항원을 주입하여 제조할 수 있으며, 주입 방법은 경구투여, 주사주입 또는 비강주입일 수 있으며, 1회 주입 또는 2 내지 4회의 반복 주입일 수 있으나 이에 한정되는 것은 아니다. 항원의 주입량은 소의 체중 1 kg당 0.001 내지 0.01 mg일 수 있으나, 이는 주입 방법 및 소의 생육단계에 따라 적절히 조절하는 것이 바람직하다. 또한 소는 도축되기 3 내지 7 주 전에 면역화시킬 수 있으며, 주입시 항체 형성을 증강시키는 보강체(Adjuvant), 예컨대 프레운드's 보강체 또는 알루미나 하이드록사이드를 동시에 주입할 수 있다. 항원을 이용한 동물면역방법은 본원발명이 속하는 기술분야의 당업자라면 용이하게 실시할 수 있으므로, 공지의 방법을 모두 적용할 수 있다. Brand cow of the present invention can be prepared by injecting the antigen by a conventional immunization method, the injection method may be oral administration, injection or nasal injection, may be a single injection or repeated injection of 2 to 4 times, but is not limited thereto. It doesn't happen. The injection amount of the antigen may be 0.001 to 0.01 mg per kg body weight of the cow, but it is preferably adjusted according to the injection method and the growth stage of the cow. Cows can also be immunized 3 to 7 weeks before slaughter and concurrently inject adjuvants such as Freund's adjuvant or alumina hydroxide that enhance antibody formation upon injection. The animal immunization method using the antigen can be easily carried out by those skilled in the art to which the present invention belongs, and thus all known methods can be applied.

본 발명의 항원은, 천연에서 분리하거나, 화학적 또는 효소학적으로 합성하거나, 또는 미생물 또는 진핵생물에서 유래한 세포로부터 유전공학적인 방법으로 제조된 것일 수 있으며, 이의 구체적인 방법은 공지의 기술로부터 용이하게 실시할 수 있다. The antigen of the present invention may be isolated from nature, chemically or enzymatically synthesized, or may be prepared by genetic engineering method from cells derived from microorganisms or eukaryotes, and specific methods thereof may be easily obtained from known techniques. It can be carried out.

본 발명의 브랜드 소 또는 쇠고기는 상기 항원에 대한 항체를 함유하고 있으므로, 상기 소 또는 쇠고기의 혈청 또는 육즙으로부터 항체의 존재를 용이하게 확인할 수 있다. 확인방법은, 면역화학방법일 수 있으며, 구체적으로는 웨스턴블롯 또는 ELISA일 수 있으나 이에 한정되는 것은 아니다. Since the brand cow or beef of the present invention contains an antibody against the antigen, the presence of the antibody can be easily confirmed from the serum or gravy of the cow or beef. The identification method may be an immunochemical method, and specifically, Western blot or ELISA, but is not limited thereto.

본 발명의 방법으로, 항체로 표지된 브랜드 소 또는 쇠고기를 간단하게 제조할 수 있을 뿐만 아니라, 소의 품질 또는 품종에 따라 상이한 항원으로 면역화시키므로써, 발현되는 항체의 종류의 식별로 소의 품질 또는 품종을 신속, 정확하게 식별할 수 있다. 또한, 한우와, 수입육을 식별할 수 있는 방법으로도 적용가능하다. The method of the present invention not only makes it easy to produce a branded cow or beef labeled with an antibody, but also immunizes with different antigens depending on the quality or breed of the cow, thereby identifying the quality or breed of the cow by identification of the type of antibody expressed. Can be identified quickly and accurately. In addition, it can be applied as a method for identifying Korean cattle and imported meat.

이에, 본 발명에서는 브랜드 소 또는 쇠고기 식별용 키트를 제공한다. 상기 키트는 브랜드 소의 제조시 사용한 항원 및 항원-항체 결합물을 검출할 수 있으며, 검출가능한 표지물로 표지된 이차항체를 포함하며, 상기 표지물에 따라, 이를 검출할 수 있는 기질을 더욱 포함할 수 있다. 상기 표지물은 금 입자 또는 효소, 예컨 대 페록시데이즈 또는 포스파테이즈일 수 있으나, 이에 한정되는 것은 아니며, 공지된 모든 종류를 사용할 수 있다. 본 발명의 키트는 96웰 플레이트 형태로 또는 스트립 형태로 제공될 수 있으나, 이에 한정되는 것은 아니다. Thus, the present invention provides a brand cow or beef identification kit. The kit may detect antigens and antigen-antibody combinations used in the preparation of the brand cow, and may include a secondary antibody labeled with a detectable label, and may further include a substrate capable of detecting the antigen, according to the label. . The label may be gold particles or enzymes, such as peroxidase or phosphatase, but is not limited thereto, and all known types may be used. The kit of the present invention may be provided in the form of a 96 well plate or in the form of a strip, but is not limited thereto.

이하 본 발명의 실시예를 기재한다. 하기 실시예는 본 발명을 예시하기 위한 것일 뿐 본 발명이 하기 실시예에 한정되는 것은 아니다.Hereinafter, examples of the present invention will be described. The following examples are only for illustrating the present invention and the present invention is not limited to the following examples.

실시예 1: 소 특이 표지성 항원의 탐색 및 선별Example 1 Screening and Screening of Bovine Specific Labeled Antigens

1-1. 항원 탐색1-1. Antigen search

여러 학술지를 검색하여 항체형성이 보고 된 펩타이드를 항원 후보로 선정하였으며(Lee, K. J. and Watson, R. D. 2002. 펩타이드s. 23:853~862; Tamar 등, 2002; Tooyama 등, 2000; Kramer, A., et al., 1998. Protein. Eng. 11(10):941~948; Mohri, H., et al., 1997. 펩타이드s. 18:1289~1293; Briand, J. P., et al., 1997. Proc. Natl. Acad. Sci. 94:12545~12550.; Benkirane, N., et al., 1995. J. Biol. Chem. 270(20):11921~11926), 한우에서는 전혀 발현되지 않는 단백질을 찾기 위해 소와 유전적인 상관성이 떨어지는 제노푸스, 초파리, 영원(Triturus) 및 애기장대를 대상으로 하였으며, 단백질을 선별하였다. Several journals were searched and peptides with reported antibody formation were selected as antigen candidates (Lee, KJ and Watson, RD 2002. Peptides s. 23: 853 ~ 862; Tamar et al., 2002; Tooyama et al., 2000; Kramer, A. , et al., 1998. Protein.Eng . 11 (10): 941-948; Mohri, H., et al., 1997. Peptides s. 18: 1289-1293; Briand, JP, et al., 1997. Proc. Natl. Acad. Sci. 94: 12545-12550; Benkirane, N., et al., 1995. J. Biol. Chem. 270 (20): 11921-11926), which are proteins that are not expressed at all in Korean cattle. We searched for genotypes, fruit flies, eternity (Triturus), and Arabidopsis, which are less genetically related to cows.

상기 선별한 단백질의 아미노산 서열을 기초로 하여 항원성 부위(epitope)를 검색하였다. 분석 프로그램으로 AntheProt와 SciProtein 등을 이용하였으며, 항원성 지수(antigenic index), 표면 가능성(surface probability), 친수성, 플랙서블 부위(flexible region) 등의 항목을 분석하였다.Antigenic epitopes were searched based on the amino acid sequences of the selected proteins. AntheProt and SciProtein were used as analysis programs, and items such as antigenic index, surface probability, hydrophilicity, and flexible region were analyzed.

상기 프로그램에서 얻어진 분석 자료를 기초로 항원으로 사용할 펩타이드 후 보군을 최종 선정하였으며, 이들 선정된 후보 펩타이드들의 프로테오소말 절단 예측(proteosomal cleavage prediction), T-세포 항원성 부위 예측 등을 웹을 기반으로 한 소프트웨어들(ProPrac, SYFPEITHI, MAPPP, PREDEP)로 분석하였다. 이후, 최종적으로 소의 단백질과 상동성이 없는 펩타이드 부위만을 항원으로 선별하였다. 최종 선택한 항원은 서열번호 1 내지 344로 기재하였다. Based on the analytical data obtained in the above program, a post-peptide post-competation group was finally selected, and proteosomal cleavage prediction and T-cell antigenic site prediction of the selected candidate peptides were based on the web. Analyzed with one software (ProPrac, SYFPEITHI, MAPPP, PREDEP). Afterwards, only peptide sites having no homology with bovine proteins were selected as antigens. The final selected antigen is set forth in SEQ ID NOs: 1-344.

1-2. 펩타이드 제조1-2. Peptide Manufacturing

상기에서 선별한 각각의 펩타이드는, (주)펩트론(대전, 대한민국)에 의뢰하여 D-형태로 합성하였다. D-형태의 아미노산이 자연계에 존재하는 L형태에 비하여 T-세포 면역반응성이 높은 것으로 알려져 있다(Van Rgenmortel, M. H. V. and Muller, S. 1998. Curr. Opin. Biotechnol. 9(4):377~382).Each peptide selected above was commissioned by Peptron (Daejeon, South Korea) and synthesized in D-form. D-type amino acids are known to have higher T-cell immunoreactivity compared to L-forms present in nature (Van Rgenmortel, MHV and Muller, S. 1998. Curr. Opin. Biotechnol. 9 (4): 377-382 ).

1-3. 운반체 단백질이 부착된 펩타이드 제조1-3. Peptide Preparation with Carrier Protein Attached

펩타이드의 크기가 작기 때문에 면역반응이 약하거나 반응을 일으키지 않을 수 있으므로 면역반응을 유발 또는 촉진시키기 위하여 분자량이 큰 단백질을 부착시켜 주었다. 면역주사를 위한 펩타이드에는 KLH(Keyhole Limpet Hemacyanin)를, ELISA 실험이나 웨스턴 블롯에 이용되는 펩타이드는 오발부민(ovalbumin)을 부착시켰다.Because of the small size of the peptide, the immune response may be weak or not occur. Therefore, a large molecular weight protein was attached to induce or promote the immune response. KLH (Keyhole Limpet Hemacyanin) was used as the peptide for immunization, and ovalbumin was attached to the peptide used in the ELISA experiment or Western blot.

실시예 2: 특이 항체로 표지된 면양 및 소 제조Example 2: Preparation of Sheep and Cattle Labeled with Specific Antibodies

2-1. 실험방법2-1. Experiment method

합성 제조한 펩타이드를 면양(Corriedale, ≒60㎏) 및 한우에게 각각 100 ㎍/㎖ 및 1 ㎎/㎖의 농도로 피하 면역주사를 실시하였다. 1차 면역주사 후 결합력 이 증진된 항체의 생성을 유도하기 위하여 3주 간격으로 2차와 3차 면역주사를 동일한 방법으로 실시하였으며(boosting), 마지막 면역주사 이후 12일경 도축하여 근육조직을 채취하였다. 채취한 근육조직을 냉동시킨 후 자연해동시킴으로써 육즙(meat juice)을 생산하며, ELISA, 웨스턴 면역블롯팅 및 점 ELISA를 실시하여 항체 검출 수준을 확인하였다. 대조구로는 면역주사하지 않은 면양 및 한우의 근육조직으로부터 분리한 육즙을 사용하였다.The synthetically prepared peptides were subjected to subcutaneous immunization at cotton (Corriedale, 60 kg) and Korean cattle at concentrations of 100 μg / ml and 1 mg / ml, respectively. In order to induce the production of antibodies with enhanced binding capacity after the first vaccination, the second and third vaccinations were performed in the same manner at three week intervals (boosting), and muscle tissues were collected by slaughtering about 12 days after the last vaccination. . The frozen muscle tissue was frozen and then thawed to produce a juice, and the antibody detection level was confirmed by ELISA, Western immunoblotting and dot ELISA. As a control, the juices isolated from the non-immunized cotton and muscle of Hanwoo were used.

가. ELISA 방법end. ELISA method

ELISA 테스트는 Flint 등(1986)과 Killefer 등(1990a, b)의 방법을 이용하여 실시하였다. 오발부민이 접합된 합성 펩타이드 각각을 TBA(tris-buffered saline: 50 mM Tris, 154 mM NaCl, pH 7.2)에 희석시킨 다음, 96-웰 플레이트에 웰 당 100 ㎕(1 ㎍/㎖)로 두었다. 저온 챔버(4 ℃)에서 하룻밤 방치한 후 플레이트에 단백질을 결합시킨 후, TBS-트윈(TBS + 0.1% Tween 20)으로 3회 세척하였다. 일차 항체로, 면양에서 생산한 비면역혈청과 항혈청, 비면역육즙 및 육즙을 각각 적정 희석배율로 희석하여 웰에 코팅된 단백질과 실온에서 2시간 배양한 후, TBS-트윈으로 다시 3회 세척하였다. 이차항체로, 10,000배 희석한 항-양 면역글로불린 G-알칼라인 포스파테이즈 접합체(Sigma Chemical, San Diego, USA)를 사용하였고, 실온에서 2시간 배양한 후 TBS-트윈으로 3회 세척하였다. ELISA tests were performed using the methods of Flint et al. (1986) and Killefer et al. (1990a, b). Each of the ovalbumin conjugated synthetic peptides was diluted in TBA (tris-buffered saline: 50 mM Tris, 154 mM NaCl, pH 7.2) and then placed in a 96-well plate at 100 μl per well (1 μg / ml). After standing overnight in a cold chamber (4 ° C.), proteins were bound to the plates and washed three times with TBS-Tween (TBS + 0.1% Tween 20). As the primary antibody, non-immune serum and antiserum, non-immune juice and gravy produced in sheep were diluted at appropriate dilution ratios, respectively, incubated with well-coated proteins at room temperature for 2 hours, and washed again with TBS-Twin three times. . As a secondary antibody, an anti-yang immunoglobulin G-alkaline phosphatase conjugate (Sigma Chemical, San Diego, USA) diluted 10,000-fold was used and incubated for 2 hours at room temperature and washed three times with TBS-twin.

반응이 끝난 각 웰에 알칼라인 포스파테이즈 기질 용액(49mM Glycine, 0.4 mM ZnCl2, 1.0 mM MgCl2, pH 9.5)에 4-NP(4-Nitro-phenyl phosphate, Sigma Chemical, San Diego, USA)를 첨가한 기질 용액을 웰당 250 ㎕씩 첨가 한 후, 실온의 암실에서 발색시켰다. 발색이 되면 0.5 M NaOH로 반응을 중지시킨 후, 마이크로플레이트 리더(Denley, Billingshurst West Sussex, UK)로 405㎚에서 흡광도 측정을 하였다. In each well, 4-NP (4-Nitro-phenyl phosphate, Sigma Chemical, San Diego, USA) was added to alkaline phosphate substrate solution (49 mM Glycine, 0.4 mM ZnCl 2 , 1.0 mM MgCl 2 , pH 9.5). 250 μl per well was added to the added substrate solution, followed by color development in the dark at room temperature. When the color was developed, the reaction was stopped with 0.5 M NaOH, and the absorbance was measured at 405 nm with a microplate reader (Denley, Billingshurst West Sussex, UK).

나. 웨스턴 면역블롯팅 방법I. Western immunoblotting method

오발부민이 접합된 펩타이드를 Laemmli(1970)의 방법에 따라 SDS-PAGE(sodium dodecyl sulfate polyacrylamide gel electrophoresis)하여 분자량에 따라 분리하였다. 분리 겔(12%)과 스텍킹 겔(3%)은 각각 4.0 ㎖ 30% 아크릴아마이드/N,N'-메틸렌-비스아크릴아마이드, 2.5 ㎖ 1.5 M Tris-HCl, pH 8.8, 3.4 ㎖ ddH2O, 100 ㎕ 10% SDS, 50 ㎕ 10% 암모늄 설페이트, 5 ㎕ N,N,N',N'-테트라메틸렌다이아민과, 1 ㎖ 30% 아크릴아마이드/N,N'-메틸렌비스아크릴아마이드, 1.25 ㎖ 1 M Tris-HCl(pH 6.8), 7.65 ㎖ ddH2O, 100 ㎕ 10% SDS, 50 ㎕ 10% 암모늄 설페이트 및 10 ㎕ TEMED로 만들었다.Ovalbumin conjugated peptide was isolated according to molecular weight by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) according to the method of Laemmli (1970). Separation gel (12%) and stacking gel (3%) were 4.0 mL 30% acrylamide / N, N'-methylene-bisacrylamide, 2.5 mL 1.5 M Tris-HCl, pH 8.8, 3.4 mL ddH 2 O, respectively. , 100 μl 10% SDS, 50 μl 10% ammonium sulfate, 5 μl N, N, N ′, N′-tetramethylenediamine, 1 mL 30% acrylamide / N, N'-methylenebisacrylamide, 1.25 Made with ml 1 M Tris-HCl, pH 6.8, 7.65 ml ddH 2 O, 100 μl 10% SDS, 50 μl 10% ammonium sulfate and 10 μl TEMED.

오발부민 접합된 펩타이드(10 ㎍)를 4x 시료 희석완충액(2% SDS, 5% β-mercaptoethanol, 62.5 mM Tris, 0.001 % bromophenol blue, 10 % glycerol)에 희석시킨 후, 90 내지 100℃의 물에 2 내지 3분간 둔 다음 상기 준비한 겔상에서 100 V에서 90분간 전기영동하였다. 이후 겔상에 전개된 단백질 밴드는 나이트로셀룰로스 막에 전사시켰다. 상기 나이트로셀룰로스 막은 증류수로 10분 정도 적신 다음, TBS-트윈(50 mM Tris-HCl, pH 7.4, 154 mM NaCl, 0.1 % Tween 20)으로 10분간 2회 세척하고, 5% 스킴 밀크/TBS-트윈으로 상온에서 1시간 정치시켰다. 이후 상기 막에 면양의 항혈청 및 생검 조직에서 생성한 육즙을 1% 스킴 밀크에 1:10,000배 희석 및 첨가하여 2시간 상온에 방치하였다. 이후 TBS-트윈 용액으로 2회 세척하고, 이차항체로 항-양 면역글로불린 G-알칼라인 포스파테이즈 접합체(Sigma Chemical, San Diego, USA)를 1% 스킴 밀크에 1:10,000으로 희석한 후 이를 반응시켰다. 상기 막에 TBS-트윈으로 3회 세척하고, 66 ㎕ NBT(Nitroblue tetrazolium, Sigma Chemical, San Diego, USA)과 33 ㎕ BCIP(5-Bromo-4-chloro-3-indoyl-phosphate, Sigma Chemical, San Diego, USA)를 함유한 10 ㎖ 알칼라인 포스파테이즈 기질 용액(100 mM Tris-HCl, 5 mM MgCl2, 100 mM NaCl, pH 9.5)을 첨가하여 암실에서 발색반응을 시켰다. 반응이 끝난 후, 증류수로 세척하여 반응을 중지시켰다.Ovalbumin conjugated peptide (10 μg) was diluted in 4 × sample dilution buffer (2% SDS, 5% β-mercaptoethanol, 62.5 mM Tris, 0.001% bromophenol blue, 10% glycerol) and then in water at 90-100 ° C. After 2-3 minutes, the gel was electrophoresed at 100 V for 90 minutes. Protein bands that then developed on the gel were transferred to nitrocellulose membranes. The nitrocellulose membrane was moistened with distilled water for about 10 minutes and then washed twice with TBS-Twin (50 mM Tris-HCl, pH 7.4, 154 mM NaCl, 0.1% Tween 20) for 10 minutes, and 5% Scheme Milk / TBS- It was allowed to stand at room temperature for 1 hour in twin. The membrane was then diluted 1: 10,000-fold in 1% skim milk and added to the antiserum and biopsy tissues of cotton sheep and left at room temperature for 2 hours. After washing twice with TBS-Twin solution, the anti-sheep immunoglobulin G-alkaline phosphatase conjugate (Sigma Chemical, San Diego, USA) was diluted 1: 10,000 in 1% skim milk as a secondary antibody and then reacted. I was. The membrane was washed three times with TBS-twin, 66 μl NBT (Nitroblue tetrazolium, Sigma Chemical, San Diego, USA) and 33 μl BCIP (5-Bromo-4-chloro-3-indoyl-phosphate, Sigma Chemical, San) Diego, USA) containing 10 ml alkaline phosphatase substrate solution (100 mM Tris-HCl, 5 mM MgCl 2, 100 mM NaCl, pH 9.5) was added to the color reaction in the dark. After the reaction, the reaction was stopped by washing with distilled water.

다. 점 ELISA 방법All. Dot ELISA Method

점 ELISA는 Macedo-Silva 등(Macedo-Silva, A., Barbosa, S. F. C., Alkmin, M. G. A., Vaz, A. J., Shimokomaki, M., Tenuta-Filho, A. 2000. Meat Sci. 56:189~192)의 방법을 수정하여 실시하였다. Point ELISA is described by Macedo-Silva et al. (Macedo-Silva, A., Barbosa, SFC, Alkmin, MGA, Vaz, AJ, Shimokomaki, M., Tenuta-Filho, A. 2000. Meat Sci. 56: 189-192). The method was modified.

실시예 1의 펩타이드를 나이트로셀룰로스 막에 5 ㎕씩 떨어뜨려 상온에서 2시간 흡착시킨 뒤, 지퍼 백에 밀폐 보관하여 4 ℃에서 하룻밤 방치하였다. 비특이적 결합을 방지하기 위하여 5 % 스킴 밀크를 사용하였으며, 항체들은 원샷(One-shot) 및 부스팅(Boosting) 처리구의 역가 측정(ELISA test)의 결과를 바탕으로 각 처리구별 가장 높은 역가를 가진 항혈청 및 육즙을 각각 1:1,000 및 1:100으로 희 석하여 사용하였다. 또한, 비 면역혈청 및 비 면역육즙도 점-ELISA를 실시해 육즙 내 항체의 반응 수준에 따른 대조군으로 사용하였다. 5 μl of the peptide of Example 1 was dropped onto the nitrocellulose membrane, and then adsorbed at room temperature for 2 hours. The peptide was kept sealed in a zipper bag and left at 4 ° C. overnight. 5% scheme milk was used to prevent nonspecific binding. Antibodies were tested for antiserum with the highest titer of each treatment based on the results of ELISA test of one-shot and boosting treatments. The juice was diluted with 1: 1,000 and 1: 100, respectively. In addition, non-immune serum and non-immune juice were also subjected to point-ELISA and used as a control according to the response level of the antibody in the juice.

2-2. 실험결과2-2. Experiment result

도 1은 면양의 비면역화된 혈청, 혈청 및 육즙 내 항체 농도를 측정한 것으로, 펩타이드를 주사하기 전(비 면역혈청)과 비교해 항체가 다량 검출됨을 확인할 수가 있었다. 상기 결과로, 특정항원을 면역주사하게되면 그 특정항원에 대한 특이항체의 생산이 가능함을 확인할 수 있다.1 is a measure of the antibody concentration in the non-immunized serum, serum and gravy of sheep, it was confirmed that a large amount of antibody compared to before the injection of the peptide (non-immune serum). As a result, it can be confirmed that the injection of specific antigens enables the production of specific antibodies to the specific antigens.

도 2는 면양의 비면역화된 혈청 및 면역화된 면양의 육즙에서의 항체 존재여부를 웨스턴 블롯으로 확인한 것으로, 항원펩타이드를 주사하지 않은 면양의 육즙(Non-immunized meat juice)에서는 면역반응을 확인할 수 없었으나, 면역주사한 면양의 육즙(Immunized meat juice)에서는 항원 제조 시 운반체 단백질로 함께 사용한 오발부민 위치(45kDa)에서 강한 항원-항체반응이 확인되었다. 상기 결과는, 면역주사를 통한 근육조직 특히, 근육조직에서 유리되는 육즙(meat juice)으로부터 항체의 검출이 가능함을 의미하며, 실험결과 처리구와 비처리구가 뚜렷히 구별되는 면역반응을 나타내어, 매우 안정적인 시스템임을 알 수 있다. 2 is a Western blot confirming the presence of antibodies in the non-immunized serum and immunized sheep juice of the sheep, it was not possible to confirm the immune response in non-immunized meat juice of non-immunized sheep However, in immunized meat juice, a strong antigen-antibody reaction was observed at the Ovalbumin site (45kDa) used as a carrier protein when the antigen was prepared. The results indicate that the antibody can be detected from free muscle juice from the muscle tissue, especially muscle tissue, through immunization, and the experimental results show a distinctly distinct immune response between the treatment and the non-treatment, resulting in a very stable system. It can be seen that.

도 3은 한우의 면역화된 혈청 및 비면역화된 혈청내의 항체 검출결과를 나타낸 것으로, 레인 1은 한우에서 얻은 혈청을 블롯한 것으로 혈청 내에 존재하는 IgG가 정상적으로 발색하는지를 확인한 대조군이다. 면역한우와 비면역한우 모두에서 진하게 발색되어 IgG에 대한 발색과정이 이상이 없음을 확인하였다. 레인 2는 항원 펩타이드-KLH 접합체를 이용한 것으로, 면역한우에서는 강한 반응이 관찰 되었으나 비면역한우에서는 약한 반응이 관찰되어, 상기 항원 펩타이드-KLH 접합체가 항체를 성공적으로 형성시킴을 알 수 있었다. 레인 3은 항원 펩타이드-오발부민 접합체를 이용한 것으로, 면역한우에서는 반응 신호가 감지되었지만 비면역한우에서는 발색이 되지 않아 항체가 형성되지 않았음을 알 수 있었다. 레인 4는 항원 펩타이드와는 아미노산 서열이 다른 펩타이드와 오발부민 접합체를 이용한 것으로, 면역한우 및 비면역한우 모두에서 항원-항체반응이 확인되지 않음을 나타낸 음성대조군이다. Figure 3 shows the results of antibody detection in the immunized serum and non-immunized serum of Hanwoo, lane 1 is a control of the blot obtained from the Hanwoo cattle and whether the IgG present in the serum normally developed. It was confirmed that there was no abnormality in the color development process for IgG due to the strong color development in both immune and non-immune cattle. In lane 2, the antigen peptide-KLH conjugate was used. A strong response was observed in the immuno-cow, but a weak reaction was observed in the non-immune cattle, indicating that the antigen-peptide-KLH conjugate successfully formed the antibody. In lane 3, antigen peptide-ovalbumin conjugate was used. The response signal was detected in the immunized cow but not developed in the non-immune cow. Lane 4 is a negative control group using an ovalbumin conjugate with a peptide having an amino acid sequence different from that of the antigenic peptide.

실시예 3: 쇠고기 식별 키트Example 3: Beef Identification Kit

도 4로 예시한 식별 키트를 제조하였다.An identification kit illustrated in FIG. 4 was prepared.

식별키트에서, "Sample pad"는 한우 육즙을 흡수하는 부위이고, "Conjugate pad"는 금 입자 결합된 항원과 금 입자 결합된 마우스 IgG를 코팅한 부위이고, "Membrane"는 분석물이 전개되는 부위이고, "Capture line"은 항원이 코팅되어 상기 항원에 특이적인 항체와 결합하는 부위이고, "Control line"은 염소 항-(마우스 IgG)가 코팅된 부위이다. In the identification kit, "Sample pad" is the site for absorbing Korean beef gravy, "Conjugate pad" is the site coated with gold particle-bound antigen and gold particle-bound mouse IgG, and "Membrane" is the site where the analyte is developed. Where "Capture line" is the site where the antigen is coated to bind to the antibody specific for the antigen, and "Control line" is the site where the goat anti- (mouse IgG) is coated.

검사원리는 선별한 항원으로 면역화된 한우에는 항원에 특이적인 항체가 존재하므로, 상기 항체의 존재 여부 확인으로 면역화된 한우(A)와 그렇지 않은 한우(B)를 분류하는 것이다. 한우 육즙을 "sample pad"에 소량 묻히면 "conjugate pad"를 지나 "membrane"을 따라 육즙의 전개가 일어난다. "Conjugate pad"에는 금 입자가 결합된 항원과, 금입자가 결합된 마우스 IgG가 건조된 상태로 코팅되어 있어, 육즙이 전개되면서 육즙 내에 존재하는 항체와 코팅된 항원사이에 면역반응이 일어나게 된다. 이때, 면역화된 한우(A)의 경우, 특이적 항체가 금 입자가 결합된 항원과 결합한 상태에서 전개되다가 "capture line"의 항원에 다시 결합하게 되므로, 금 입자 결합된 항원-항체 결합물이 "capture line"에 결합되어 육안으로 식별할 수 있는 띠를 형성한다. 반면 비면역화된 한우(B)의 경우, 특이적 항체가 존재하지 않으므로 "capture line"에는 띠가 형성되지 않는다. "Control line"에는 금 입자 결합된 마우스 IgG를 포획할 수 있는 염소 항-(마우스 IgG) 항체가 코팅되어 있어, 반응이 정상적으로 진행될 경우 육안으로 확인할 수 있는 띠가 형성된다(도 5 및 6).Principle of the test is that the Korean native cows immunized with the selected antigens have antibodies specific for the antigens. Therefore, the immunized Korean cattle (A) and the Koreans (B) are not classified by the presence of the antibody. A small amount of Korean beef gravy is placed on the "sample pad", passing through the "conjugate pad" and along the "membrane". The "conjugate pad" is coated with an antigen in which gold particles are bound and a mouse IgG in which gold particles are bound. As the juice develops, an immune reaction occurs between the antibody and the coated antigen in the juice. In this case, in the case of immunized Hanwoo (A), the specific antibody is developed in the state of binding with the antigen to which the gold particles are bound, and then binds back to the antigen of the "capture line". coupled to the capture line "to form a visible band. On the other hand, in the case of non-immunized Hanwoo (B), since no specific antibody is present, no band is formed in the "capture line". The "Control line" is coated with goat anti- (mouse IgG) antibody capable of capturing gold particle bound mouse IgG, so that a band is visible to the naked eye when the reaction proceeds normally (FIGS. 5 and 6).

이상 살펴본 바와 같이, 본 발명은 항체로 표지된 브랜드 소 또는 쇠고기를 간단하게 제조할 수 있을 뿐만 아니라, 소의 품질 또는 품종에 따라 상이한 항원으로 면역화시키므로써, 발현되는 항체의 종류의 식별로 소의 품질 또는 품종을 신속, 정확하게 식별할 수 있다. 또한, 한우와, 수입육을 식별할 수 있는 방법으로도 적용가능하다. As described above, the present invention can not only easily produce a branded cow or beef labeled with an antibody, but also immunize with different antigens according to the quality or breed of the cow, thereby identifying the type of the antibody expressed or the quality of the cow. Breeds can be identified quickly and accurately. In addition, it can be applied as a method for identifying Korean cattle and imported meat.

<110> YEUNGNAM EDUCATIONAL FOUNDATION <120> PREPARATION METHOD FOR BRAND MEAT AND IDENTIFICATION METHOD FOR SAME <130> dpp20042285kr <160> 344 <170> KopatentIn 1.71 <210> 1 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K000-1 peptide <400> 1 Lys Ser Ile Ala Ser Ser Ser Ser Trp Met His Ser Asn Gly Asp Glu 1 5 10 15 Gly Ser Ser Arg Pro Gly Val 20 <210> 2 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K000-2 peptide <400> 2 Arg Val Leu Phe Pro Asp Asn Met Lys Asp Ser Ser Thr Ser Ser Gly 1 5 10 15 Glu Gln Gly Arg Asn Met Val 20 <210> 3 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K000-3 peptide <400> 3 Phe Ile Asp Thr Tyr Gly His Val Thr Ser His Gly Asn Asp Asp Asn 1 5 10 15 Gly Gly Phe Pro Glu Gln Gln 20 <210> 4 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K000-4 peptide <400> 4 Lys Arg His Arg Asp Leu Leu Ser Pro Val Leu Asp Arg Arg Lys Asp 1 5 10 15 Lys Lys Leu Lys Arg Ala Ala 20 <210> 5 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K000-5 peptide <400> 5 Glu Gly Asp Asp Cys Met Thr Ser Arg Pro Ser Glu Ser Pro Glu Asp 1 5 10 15 Lys Asn Ile Cys Ala Ser Pro 20 <210> 6 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K001-1 peptide <400> 6 Ala Leu Arg Glu Gln Leu Asp Lys Phe Asn Lys Gln Gln Glu Lys Cys 1 5 10 15 Gln Ser Thr Leu Ser Ser Ile 20 <210> 7 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> K001-2 peptide <400> 7 Thr Ala Leu Ser Arg Ser Tyr Val Pro Ala Ala Thr Thr Ser Gln Lys 1 5 10 15 Pro Asn Val Phe Arg Gly 20 <210> 8 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K001-3 peptide <400> 8 Gln Leu His Gly Val Ser Asn Lys Pro Lys Asp Lys Lys Lys Lys Lys 1 5 10 15 Lys Glu Ile Thr Asn Arg Thr 20 <210> 9 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K002-1 peptide <400> 9 Ala Lys Thr Phe Tyr Asp Asn Tyr Thr Pro Lys Asn Asn Asn Gln Lys 1 5 10 15 Lys Ser Ala Lys Asn Gly Ser 20 <210> 10 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> K002-2 peptide <400> 10 Tyr Thr Pro Lys Asn Asn Asn Gln Lys Lys Ser Ala Lys Asn Gly Ser 1 5 10 15 Gly Gly <210> 11 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K002-3 peptide <400> 11 Glu Ala Lys Ala Asp Asp Gly Gly Ser Ser Met Glu Asn Gly Asp Asp 1 5 10 15 Asp Arg Asn Leu Lys Asp Glu 20 <210> 12 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K002-4 peptide <400> 12 Ala Ile Leu Glu Glu Gln Glu Leu Ala Glu Lys Leu Asn Gln Gln Glu 1 5 10 15 Ser Ala Gly Lys Val Thr Thr 20 <210> 13 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K002-5 peptide <400> 13 Ser Asp Arg Gln Val Gly Met Lys Ser Lys Arg Glu Glu Glu Glu Glu 1 5 10 15 Glu Glu Ala Ser Val Ala Ala 20 <210> 14 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K002-6 peptide <400> 14 Tyr Lys Val Asp Leu Asn Val Glu Ala Glu Glu Ala Glu Gln Asp Glu 1 5 10 15 Asn Asp Val Asp Trp Gln Glu 20 <210> 15 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K003-1 peptide <400> 15 Val Lys Leu Val Arg Leu Leu Val Arg Ile Phe Tyr Asp Asn Tyr Thr 1 5 10 15 Pro Glu Ser Asp Asn Gln Gln 20 <210> 16 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> K003-2 peptide <400> 16 Leu Leu Val Arg Ile Phe Tyr Asp Asn Tyr Thr Pro Glu Ser Asp Asn 1 5 10 15 Gln Gln <210> 17 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K003-3 peptide <400> 17 Asn Val Asp Gly Glu Ala Ala Lys Leu Ser Asp Asp Lys Lys Ser Asp 1 5 10 15 Glu Tyr Leu Lys Ala Tyr Tyr 20 <210> 18 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K003-4 peptide <400> 18 Thr Tyr Ser Asp Arg Gln Val Gly Met Lys Ser Lys Arg Glu Glu Glu 1 5 10 15 Glu Glu Asp Val Glu Trp Glu 20 <210> 19 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K003-5 peptide <400> 19 Asn Val Glu Ala Glu Glu Ala Glu Glu Lys Glu Asp Gly Asp Glu Asp 1 5 10 15 Asp Asp Ile Asp Trp Glu Glu 20 <210> 20 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K004-1 peptide <400> 20 Cys Gln Ser Thr Leu Ser Ser Ile Ala Ser Ser Arg Glu Arg Thr Ser 1 5 10 15 Ser Ser Arg Gln Asn Val Pro 20 <210> 21 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K004-2 peptide <400> 21 Ser Thr Leu Ser Ser Ile Ala Ser Ser Arg Glu Arg Thr Ser Ser Ser 1 5 10 15 Arg Gln Asn Val Pro Leu Pro 20 <210> 22 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K004-3 peptide <400> 22 Arg Arg Ala Ala Ala Gln Thr His Gly Ile Ser Asn Lys Pro Lys Asp 1 5 10 15 Lys Lys Lys Lys Lys Gln Glu 20 <210> 23 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K004-4 peptide <400> 23 Ala Gln Thr His Gly Ile Ser Asn Lys Pro Lys Asp Lys Lys Lys Lys 1 5 10 15 Lys Gln Glu Ile Ser Lys Arg 20 <210> 24 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K005-1 peptide <400> 24 Phe Leu Asp Leu Ala Tyr Phe Glu Gly Val Lys Thr Glu Ser Tyr Asp 1 5 10 15 Glu Asn Val Leu Ile Asp Ile 20 <210> 25 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K005-2 peptide <400> 25 Ser Val Gly Lys Val Asp Asp Ile Met Ala Gln Asn Lys Pro Asn Lys 1 5 10 15 Lys Leu Glu Ile Gln Ile Arg 20 <210> 26 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K005-3 peptide <400> 26 Ala Asp Phe Arg Leu Lys Arg Ser Thr Ser Ile Pro Lys Tyr Ser Ser 1 5 10 15 Ser Thr Phe Glu Ile Lys Phe 20 <210> 27 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K005-4 peptide <400> 27 Ser Thr Val Ala Arg Phe Pro Tyr Val Cys Arg Glu Gly Glu Thr Asp 1 5 10 15 Tyr Glu Ala Ile Tyr Val Thr 20 <210> 28 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K006-1 peptide <400> 28 Arg Thr Lys Cys Leu Leu Asn Asn Ala Glu Ile Pro Pro His His Pro 1 5 10 15 Thr Asp Pro Val Glu Met Arg 20 <210> 29 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K006-2 peptide <400> 29 Cys Leu Leu Asn Asn Ala Glu Ile Pro Pro His His Pro Thr Asp Pro 1 5 10 15 Val Glu Met Arg Arg Ile Asn 20 <210> 30 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K007-1 peptide <400> 30 Leu Glu Gln Ile Leu Glu Ala Ile Asn Gln Gly Thr Arg Arg His Arg 1 5 10 15 Arg Gln Thr Ser Lys Ile Lys 20 <210> 31 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> K007-2 peptide <400> 31 Leu Ser Tyr Thr Cys Phe Val Leu Ala Ala Leu Glu Asp Gly Asp Thr 1 5 10 15 Lys Lys Tyr Ala Ala Ser 20 <210> 32 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K007-3 peptide <400> 32 Asp Asp Ile Val Val Glu Val Ser Ser Leu Ala Lys Gln Gln Glu Glu 1 5 10 15 Pro Glu Met Leu Trp Val Met 20 <210> 33 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K007-4 peptide <400> 33 Ile Ile Ile Ile Val Ile Ala Ile Leu Leu Phe Lys Arg Lys Arg Thr 1 5 10 15 Asn Ser Pro Pro Gly Lys Asp 20 <210> 34 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K008-1 peptide <400> 34 Lys Ile Lys Gly Tyr Tyr Ile Val Ile Val Pro Leu Lys Lys Ser Arg 1 5 10 15 Gly Lys Phe Ile Lys Pro Trp 20 <210> 35 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K008-2 peptide <400> 35 Phe Ile Ile Cys Ile Val Ile Ala Ile Leu Leu Tyr Lys Arg Lys Arg 1 5 10 15 Thr Glu Ser Asp Ser Arg Lys 20 <210> 36 <211> 25 <212> PRT <213> Artificial Sequence <220> <223> K008-3 peptide <400> 36 Ile Val Ile Ala Ile Leu Leu Tyr Lys Arg Lys Arg Thr Glu Ser Asp 1 5 10 15 Ser Arg Lys Ser Ser Leu Pro Asn Ser 20 25 <210> 37 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K009-1 peptide <400> 37 Gly Gly His Glu Lys Leu Leu Leu Pro Gly Leu Gln Gly Thr Asp Lys 1 5 10 15 Gly Ser Gly Leu Ala Gly Gly 20 <210> 38 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K009-2 peptide <400> 38 Gly Ala Gly Gly Gly Gly Tyr Val Gln Ile Ile Ser Ser Pro Gly Pro 1 5 10 15 Pro Leu His His His Gly Leu 20 <210> 39 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K009-3 peptide <400> 39 Ala Arg Ala Gly Gln Leu Gln Pro Val Leu Gly Arg Pro Pro Ala Lys 1 5 10 15 Arg Arg Leu Met Glu Asn Val 20 <210> 40 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> K010-1 peptide <400> 40 Met Ile Tyr Met Val Ser Val Ser Met Met Val Leu Val Leu 1 5 10 <210> 41 <211> 17 <212> PRT <213> Artificial Sequence <220> <223> K010-2 peptide <400> 41 Met Val Ser Val Ser Met Met Val Leu Val Leu Gly Leu Val Ala Val 1 5 10 15 Ala <210> 42 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> K010-3 peptide <400> 42 Ile Asn Phe Val Cys Gly Ile Trp Val Asn Ser Lys Ser Met Trp Trp 1 5 10 15 Glu Ser Ser Cys Val 20 <210> 43 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K011-1 peptide <400> 43 Phe Asn Met Asn Ile Met Asn Leu His His Leu Ile Lys Thr Ser Val 1 5 10 15 Lys Thr Ala Phe Leu Ile Ser 20 <210> 44 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K012-1 peptide <400> 44 Leu Thr Ala Ser Tyr Ser Leu Tyr Met Phe Leu Met Thr Gln Arg Gly 1 5 10 15 Met Thr Pro Glu His Leu Asn 20 <210> 45 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K012-2 peptide <400> 45 Arg Gly Met Thr Pro Glu His Leu Asn Ala Ile Asn Pro Thr His Thr 1 5 10 15 Arg Glu His Thr Leu Met Thr 20 <210> 46 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> K013-1 peptide <400> 46 Ser Thr Ile Leu Ala Ile Leu Ser Phe Trp Leu Pro Gln Met Thr Pro 1 5 10 15 Asp Met <210> 47 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K013-2 peptide <400> 47 Ile Ala Leu Leu Leu Pro Phe Pro Trp Ala Ala Gln Leu Asn Thr Pro 1 5 10 15 Ser Ile Val Ile Leu Trp Ala 20 <210> 48 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K014-1 peptide <400> 48 Asp Pro Phe Thr Leu Ile Ser Trp Pro Ile Gln Ser Asn Gly Phe Asn 1 5 10 15 Asn Arg Leu Ile Thr Leu Gln 20 <210> 49 <211> 29 <212> PRT <213> Artificial Sequence <220> <223> K014-2 peptide <400> 49 Leu His Asn Phe Thr Thr Ile Phe Tyr Gln Leu Thr Ser Pro Gly His 1 5 10 15 Lys Trp Ala Leu Leu Leu Thr Ser Leu Met Leu Leu Leu 20 25 <210> 50 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K015-1 peptide <400> 50 Met Met Met Phe Ile Ile Trp Glu Ala Phe Ala Ala Lys Arg Glu Val 1 5 10 15 Thr Thr Tyr Glu Leu Thr Ser 20 <210> 51 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K015-2 peptide <400> 51 Ala Phe Ala Ala Lys Arg Glu Val Thr Thr Tyr Glu Leu Thr Ser Thr 1 5 10 15 Met Leu Glu Trp Leu Gln Gly 20 <210> 52 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K015-3 peptide <400> 52 Tyr Glu Leu Thr Ser Thr Met Leu Glu Trp Leu Gln Gly Cys Pro Thr 1 5 10 15 Pro Tyr His Thr Leu Lys Thr 20 <210> 53 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> K016-1 peptide <400> 53 Met Asn Pro Ile Thr Phe Ser Val Val Leu Thr Ser Leu Ala Ser Glu 1 5 10 15 Gln Phe Leu Ala Val Ser 20 <210> 54 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> K016-2 peptide <400> 54 Val Leu Thr Ser Leu Ala Ser Glu Gln Phe Leu Ala Val Ser Ser Ser 1 5 10 15 His Trp Leu Leu Ala Trp 20 <210> 55 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K016-3 peptide <400> 55 Gly Phe Val Pro Lys Trp Phe Ile Ile Gln Glu Leu Thr Ser Gln Asn 1 5 10 15 Thr Thr Ile Leu Ala Thr Thr 20 <210> 56 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K016-4 peptide <400> 56 Phe Tyr Leu Arg Leu Thr Tyr Ile Val Thr Leu Thr Ser Ser Pro Asn 1 5 10 15 Thr Ser Asn Ala Ser Leu Thr 20 <210> 57 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K016-5 peptide <400> 57 Ser Pro Asn Thr Ser Asn Ala Ser Leu Thr Trp Arg His His Ser Lys 1 5 10 15 Gln Pro Thr Leu Leu Leu Ser 20 <210> 58 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K017-1 peptide <400> 58 Ile Val Leu Val Leu Gly Leu Val Ala Val Ala Ser Asn Pro Ser Pro 1 5 10 15 Phe Tyr Ala Ala Leu Gly Leu 20 <210> 59 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-1 peptide <400> 59 His Asn Lys Ile Pro Pro Arg Trp Leu Asn Cys Pro Arg Arg Gly Gln 1 5 10 15 Pro Val Ala Ala Lys Phe Leu 20 <210> 60 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-2 peptide <400> 60 Leu Pro Leu Lys Thr Met Leu Gly Pro Lys Tyr Asp Asp Gln Val Pro 1 5 10 15 Glu Glu Asn Arg Phe His Pro 20 <210> 61 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-3 peptide <400> 61 Lys Pro Pro Glu Leu Pro Asp Trp Cys Phe Glu Glu Glu Asp Val Asp 1 5 10 15 Asp Glu Gly Asn Asn Val Phe 20 <210> 62 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-4 peptide <400> 62 Glu Ala Glu Ala Gly Ser Ser Gly Ala Thr Tyr Asn Arg Arg Lys Lys 1 5 10 15 Glu Arg Leu Lys Leu Gly Ala 20 <210> 63 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-5 peptide <400> 63 Gly Leu Leu Thr Arg Asn Val Gly Leu Leu Tyr Val Gly Lys Tyr Asp 1 5 10 15 Cys Pro Phe Ser Glu Ile Lys 20 <210> 64 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-6 peptide <400> 64 Val Gly Leu Leu Tyr Val Gly Lys Tyr Asp Cys Pro Phe Ser Glu Ile 1 5 10 15 Lys Asp Leu Tyr Leu Met Gly 20 <210> 65 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K019-1 peptide <400> 65 His Asn Lys Ile Pro Pro Arg Trp Leu Asn Cys Pro Arg Arg Gly Gln 1 5 10 15 Pro Val Ala Ala Lys Phe Leu 20 <210> 66 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K019-2 peptide <400> 66 Leu Pro Leu Lys Thr Met Leu Gly Pro Lys Tyr Asp Asp Gln Val Pro 1 5 10 15 Glu Glu Asn Arg Phe His Pro 20 <210> 67 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K019-3 peptide <400> 67 Pro Lys Pro Pro Glu Leu Pro Asp Trp Cys Phe Glu Glu Glu Asp Val 1 5 10 15 Asp Asp Glu Gly Asn Asn Val 20 <210> 68 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K020-1 peptide <400> 68 His Asn Lys Ile Pro Pro Arg Trp Leu Asn Cys Pro Arg Arg Gly Gln 1 5 10 15 Pro Val Ala Ala Lys Phe Leu 20 <210> 69 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K020-2 peptide <400> 69 Leu Pro Leu Lys Thr Met Leu Gly Pro Lys Tyr Asp Asp Gln Val Pro 1 5 10 15 Glu Glu Asn Arg Phe His Pro 20 <210> 70 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K020-3 peptide <400> 70 Lys Pro Pro Glu Leu Pro Asp Trp Cys Phe Glu Glu Glu Asp Val Asp 1 5 10 15 Asp Glu Gly Asn Asn Val Phe 20 <210> 71 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K020-4 peptide <400> 71 Glu Ala Glu Ala Gly Ser Ser Gly Ala Thr Tyr Asn Arg Arg Lys Lys 1 5 10 15 Glu Arg Leu Lys Leu Gly Ala 20 <210> 72 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K020-5 peptide <400> 72 Asp Phe Ile Asp Arg Cys Thr Ala Ala Asn His Arg Gln Asn Arg Lys 1 5 10 15 Asn Pro Leu Asp Pro Asp Ala 20 <210> 73 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> K021-1 peptide <400> 73 Leu Leu Pro Leu Leu Ser Gly Val His Cys Asp Val Gln Leu Ala Gln 1 5 10 15 Ser Glu Ser <210> 74 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K022-1 peptide <400> 74 Ala Lys Leu Lys Ala Asp Tyr Asn Leu Pro Met His Lys Met Thr Asn 1 5 10 15 Thr Asp Leu Thr Arg Ile Leu 20 <210> 75 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K022-2 peptide <400> 75 Gln Arg Ala Leu Arg Ala Pro Asn Lys Lys Val Lys Arg Arg Glu Leu 1 5 10 15 Lys Lys Asn Pro Leu Lys Asn 20 <210> 76 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-1 peptide <400> 76 Ser Leu Arg Pro Pro Val Pro Gly Gln Glu Thr Asn Asn Phe Lys Pro 1 5 10 15 Glu Lys Phe Thr Met Asp Ser 20 <210> 77 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-2 peptide <400> 77 Lys Phe Thr Met Asp Ser Gln His Leu Lys His Lys Glu Asp Phe Asn 1 5 10 15 Arg Glu Ala Glu Gly Cys Val 20 <210> 78 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-3 peptide <400> 78 Thr Asp Gly Ser Val Asp Gly Ile Pro Pro Tyr Arg Ser Lys Lys Gln 1 5 10 15 Arg Glu Ile His Arg Ser Val 20 <210> 79 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-4 peptide <400> 79 Ala Lys Leu Thr Ile Ala Leu Glu Lys Val Lys Lys Gln Arg Asp Ala 1 5 10 15 Glu Glu Lys Leu Glu Glu Lys 20 <210> 80 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-5 peptide <400> 80 Val Gln Arg Lys Gly Lys Phe Gln Pro Ala Phe Ser Lys Gly Gln Thr 1 5 10 15 Ser Thr Ser Cys His Leu Arg 20 <210> 81 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-6 peptide <400> 81 Thr Ser Ala Ala Val Leu Thr Thr Pro Leu Ser Pro Glu Gln Glu Ala 1 5 10 15 Glu Arg Asn His Ser Val Leu 20 <210> 82 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-7 peptide <400> 82 Lys Ser Arg Cys Val Gln Ser Thr Thr Leu Lys Glu Lys Gly Lys Thr 1 5 10 15 Ala Glu Ser Val Pro Ser Ser 20 <210> 83 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-8 peptide <400> 83 Val Pro Ser Ser Gly Phe Ser Thr Leu Lys Leu Ser Glu Glu Gln Lys 1 5 10 15 Thr Ala Lys Lys Pro Ser Ser 20 <210> 84 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K024-1 peptide <400> 84 Glu Pro Val Phe Glu Ser Leu Glu Pro Tyr Lys Gly Pro Gly Arg Glu 1 5 10 15 Asp Asn Ala Met Gln Ser Pro 20 <210> 85 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K024-2 peptide <400> 85 Ser Val Arg Ala Tyr Leu Gly Tyr Gln Thr Val Pro Ser Gly Ser Ser 1 5 10 15 Gly Asn Leu Ser Ser Ala Ser 20 <210> 86 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K024-3 peptide <400> 86 Ser Gly Ser Ser Gly Asn Leu Ser Ser Ala Ser Ser Ser Ser Ser Pro 1 5 10 15 Pro Gly Thr Pro Asn Pro Leu 20 <210> 87 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K024-4 peptide <400> 87 Ser Ser Ser Pro Pro Gly Thr Pro Asn Pro Leu Asp Ser Lys Ser Glu 1 5 10 15 Gly Pro Ser Gly Ala Ser Gly 20 <210> 88 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K024-5 peptide <400> 88 Glu Gly Pro Ser Gly Ala Ser Gly Thr Gly Tyr Arg Lys Ser Gly Ser 1 5 10 15 Gly Lys Ala Lys Lys Ser Leu 20 <210> 89 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> K024-6 peptide <400> 89 Ser Gly Ser Gly Lys Ala Lys Lys Ser Leu Asp Lys Gln Ser Asn Asp 1 5 10 15 Tyr Lys Leu Arg 20 <210> 90 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K025-1 peptide <400> 90 Ser His Leu Leu Gln Arg Cys Ser Ala Gln Leu Cys Thr Arg Gly Ser 1 5 10 15 Ser Leu Ile Leu Ser Leu Leu 20 <210> 91 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K025-2 peptide <400> 91 Leu Leu Leu Ser Val Cys Leu Ser Val Glu Gly Gln Tyr Asn Gly Glu 1 5 10 15 Lys Gly Ile Ser Ile Pro Asp 20 <210> 92 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K025-3 peptide <400> 92 Pro Ala Val Pro Glu Thr Trp Thr Ser Asn Ser Arg Thr Tyr Tyr Arg 1 5 10 15 Asp Lys Phe Met Cys Pro Arg 20 <210> 93 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K025-4 peptide <400> 93 Lys Val Pro Ala Tyr Val Asn Tyr His Phe Leu Gly Glu Lys Asp Cys 1 5 10 15 Gly Ala Pro Cys Glu Val Gly 20 <210> 94 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K026-1 peptide <400> 94 Asp Gly Asp Ser Lys Ala Glu Lys Ala Lys Ala Lys Asp Glu Pro Gln 1 5 10 15 Arg Arg Ser Ala Arg Leu Ser 20 <210> 95 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K026-2 peptide <400> 95 Asp Lys Ala Pro Lys Gly Lys Lys Gly Lys Ala Asp Ser Gly Lys Asp 1 5 10 15 Ser Ser Asn Ala Ala Glu Asn 20 <210> 96 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 96 Arg Lys Gln Val Asn Ala Asp Val Ala Asp Ala Lys Asp Glu Pro Lys 1 5 10 15 Arg Arg Ser Ala Arg Leu Ser 20 <210> 97 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 97 Leu Ser Ser Lys Pro Thr Pro Ala Lys Thr Glu Pro Lys Pro Lys Lys 1 5 10 15 Glu Lys Ala Pro Val Lys Glu 20 <210> 98 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 98 Pro Lys Lys Glu Lys Ala Pro Val Lys Glu Lys Pro Glu Glu Lys Glu 1 5 10 15 Lys Lys Val Pro Ala Lys Gly 20 <210> 99 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 99 Glu Glu Ala Asn Lys Glu Glu Ala Asn Glu Asp Gln Pro Ser Glu Asn 1 5 10 15 Gly Glu Thr Lys Ser Asp Glu 20 <210> 100 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 100 Lys Arg Lys Arg Glu Trp Ser Asp Glu Ser Glu Glu Glu Pro Glu Lys 1 5 10 15 Glu Leu Ala Pro Glu 20 <210> 101 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 101 Phe Cys Trp Lys Arg Lys Met Glu Trp Trp Asp Lys Ser Glu Glu Ser 1 5 10 15 Glu Glu Glu Pro Arg Lys Val 20 <210> 102 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 102 Phe Leu Ala Leu Tyr Leu Ala Asn Asp Met Glu Glu Asp Asp Glu Asp 1 5 10 15 Ser Lys Gln Asn Ile Phe His 20 <210> 103 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 103 Ser Asp Gly Thr Thr Ser Arg Lys Leu Arg Tyr Ser His His Asp Lys 1 5 10 15 Lys Asn Gly Arg Ser Ser Asn 20 <210> 104 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 104 Arg Lys Leu Arg Tyr Ser His His Asp Lys Lys Asn Gly Arg Ser Ser 1 5 10 15 Asn Gly Ala Leu Arg Gly Val 20 <210> 105 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 105 Leu Glu Ile Lys Ala Cys Val Leu Met Asn Met Lys Glu Lys Gly Thr 1 5 10 15 Glu Thr Val Lys Ala Glu Lys 20 <210> 106 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 106 Met Gly Arg Lys Lys Lys Leu Pro Thr Gly Val Ser Ser Gly 1 5 10 <210> 107 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 107 Asn Leu Glu Met Val Glu Leu Leu Val Val Met Gly Val Glu Thr Lys 1 5 10 15 Asp Ala Leu Leu His Ala Ile 20 <210> 108 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 108 Glu Leu Leu Leu Glu His Glu Glu Leu Ile Tyr Lys Glu Gly Glu Pro 1 5 10 15 Tyr Ser Trp Gln Lys Val Asp 20 <210> 109 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 109 Leu Glu His Glu Glu Leu Ile Tyr Lys Glu Gly Glu Pro Tyr Ser Trp 1 5 10 15 Gln Lys Val Asp Ile Asn Thr 20 <210> 110 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 110 Leu Ser Trp Glu Leu Arg Asn Leu Ala Leu Thr Glu Gln Glu Cys Lys 1 5 10 15 Ser Glu Tyr Met Asp Leu Arg 20 <210> 111 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 111 Gln Cys Gln Lys Phe Ala Val Asp Leu Leu Asp Gln Thr Arg Thr Ser 1 5 10 15 Asn Glu Leu Ala Ile Ile Leu 20 <210> 112 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 112 Arg Met Ser Leu Thr Arg Leu Val Gln Ala Ile Ser Tyr Lys Gln Lys 1 5 10 15 Lys Phe Val Ala His Ser Asn 20 <210> 113 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 113 Ser Ser Lys Thr Ile Asp Arg Gln Arg Ser Lys Lys Arg Lys Glu Gln 1 5 10 15 Glu Gln Phe Ser Glu Tyr Asp 20 <210> 114 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 114 Glu Asn Ser Gly Met Asp Val Ser Ser Ala Asn Lys Lys Glu Arg Gln 1 5 10 15 Pro Arg Pro Arg Arg Ile Lys 20 <210> 115 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 115 Ser Gly Arg Glu Ser Pro Ile Arg Ile Glu Phe Glu Asp Glu Lys Thr 1 5 10 15 Ser Thr Leu Leu Glu Leu Leu 20 <210> 116 <211> 24 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 116 Arg Ser Leu Thr Ala Pro Glu Leu Lys Ile Ser Arg Lys Ser Ser Pro 1 5 10 15 Ala Pro Thr Pro Thr Pro Thr Pro 20 <210> 117 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 117 Ser Asp Ile Ser Ser Ile Ala Ser Thr Ser Pro Gln Arg Pro Lys His 1 5 10 15 Arg Asn <210> 118 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 118 Pro Gln Leu Gln Arg Ile Gln Pro Tyr Leu Val Pro Gln Arg Asn Arg 1 5 10 15 Ser Gln Ala Gly Gly Leu Leu 20 <210> 119 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 119 Gln Pro Ser Leu Leu Ser Ser Ala Leu Met Pro Lys Arg Lys Arg Gly 1 5 10 15 Arg Pro Arg Lys Leu Ser Gly 20 <210> 120 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 120 Asn Gly Lys Met Cys Asn Glu Ser Tyr Ser Gly Asn Asp Asp Gly Ser 1 5 10 15 Asp Asp Asn Gln Pro Asn Ala 20 <210> 121 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 121 Asp Leu Asn Glu Ser Arg Asp Ser Leu Pro Ser Lys Arg Ser Lys Asn 1 5 10 15 Ser Lys Asp His Arg Val Val 20 <210> 122 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 122 Gln Ser Leu Pro Ala Thr Ser Asn Ala Leu Ser Thr Ser Ser Ser Ser 1 5 10 15 Ser Thr Ser Ser Ser Ser Gly 20 <210> 123 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 123 Pro Gln Leu Gln Arg Ile Gln Pro Tyr Leu Val Pro Gln Arg Asn Arg 1 5 10 15 Ser Gln Ala Gly Gly Leu Leu 20 <210> 124 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 124 Pro Ser Leu Leu Ser Ser Ala Leu Met Pro Lys Arg Lys Arg Gly Arg 1 5 10 15 Pro Arg Lys Leu Ser Gly Ser 20 <210> 125 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 125 Gly Lys Met Cys Asn Glu Ser Tyr Ser Gly Asn Asp Asp Gly Ser Asp 1 5 10 15 Asp Asn Gln Pro Asn Ala Gly 20 <210> 126 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 126 Asp Leu Asn Glu Ser Arg Asp Ser Leu Pro Ser Lys Arg Ser Lys Asn 1 5 10 15 Ser Lys Asp His Arg Val Val 20 <210> 127 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 127 Gln Ser Leu Pro Ala Thr Ser Asn Ala Leu Ser Thr Ser Ser Ser Ser 1 5 10 15 Ser Thr Ser Ser Ser Ser Gly 20 <210> 128 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 128 Pro Gln Leu Gln Arg Ile Gln Pro Tyr Leu Val Pro Gln Arg Asn Arg 1 5 10 15 Ser Gln Ala Gly Gly Leu Leu 20 <210> 129 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 129 Gln Pro Ser Leu Leu Ser Ser Ala Leu Met Pro Lys Arg Lys Arg Gly 1 5 10 15 Arg Pro Arg Lys Leu Ser Gly 20 <210> 130 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 130 Pro Lys Arg Lys Arg Gly Arg Pro Arg Lys Leu Ser Gly Ser Ser Asn 1 5 10 15 Gly Thr Gly Asn Asp Tyr Asp 20 <210> 131 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 131 Lys Arg Gly Arg Pro Arg Lys Leu Ser Gly Ser Ser Asn Gly Thr Gly 1 5 10 15 Asn Asp Tyr Asp Asp Phe Asp 20 <210> 132 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 132 Arg Pro Arg Lys Leu Ser Gly Ser Ser Asn Gly Thr Gly Asn Asp Tyr 1 5 10 15 Asp Asp Phe Asp Arg Glu Asn 20 <210> 133 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 133 Asn Asn Ser Met Leu Asn Thr Thr Asp Tyr Tyr Gln Gln Gln Gln Gln 1 5 10 15 Glu Ser Pro Ser Ser Ala Gly 20 <210> 134 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 134 Met Asp Asp Asp Leu Glu Leu Leu Ser Leu Asn Asp Gln Gln Asp Lys 1 5 10 15 Ser Asp Glu Pro Asp His Glu 20 <210> 135 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 135 Met Ala Ile Met Ile Val Ile Leu Cys Arg Met Gln Thr Pro His Ser 1 5 10 15 Lys Gln Thr Leu Gln Thr Pro 20 <210> 136 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 136 Ala Lys Phe Pro Leu Ile Arg Gln Phe Ser Leu Glu Ser Ser Ser Ser 1 5 10 15 Gly Lys <210> 137 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 137 Ile Asp Asn Leu Leu Gln Glu Met Leu Leu Gly Gly Ser Ala Asn Glu 1 5 10 15 Ala Ser His Thr His His His 20 <210> 138 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 138 Ser Gln Leu His Asn Gly Gln Met Ser Thr Pro Glu Thr Pro Gln Pro 1 5 10 15 Ser Pro Pro Ala Gly Ser Gly 20 <210> 139 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 139 Gly Thr Ile Ala Ser Ile Asn Lys Gln Pro Thr Ser Ile Pro Gln Ser 1 5 10 15 Thr Ile Thr Lys Gln Glu Ala 20 <210> 140 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 140 Gln Gly Met Gly Ile Cys Ala Gln Cys Pro Ala Asn Ser Arg Ser Thr 1 5 10 15 Ser Glu Ala Ser Pro Ile Cys 20 <210> 141 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 141 Ile Ile Val Ile Val Cys Phe Ser Arg Lys Gln Arg Asn Asp Ser Glu 1 5 10 15 Ser Glu Tyr Thr 20 <210> 142 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 142 His Lys Met Thr Asn Thr Asp Leu Thr Arg Ile Leu Lys Ser Gln Glu 1 5 10 15 Ile Gln Arg Ala Leu Arg Ala 20 <210> 143 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 143 Ile Leu Gln Gln Leu Glu Asn Ile Lys Ala Lys Glu Lys Lys Pro Asp 1 5 10 15 Asp Gly Lys Pro Lys Ala Lys 20 <210> 144 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 144 Lys Tyr Ser Gln Tyr Ile Pro Ala Asn Arg Tyr Pro Lys Asn Glu Asp 1 5 10 15 Thr Tyr Pro Glu Lys 20 <210> 145 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 145 Asn Arg Tyr Pro Lys Asn Glu Asp Thr Tyr Pro Glu Lys Thr Ser Glu 1 5 10 15 Thr Ala Thr Ala Pro Phe Gln 20 <210> 146 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 146 Asn Phe Asn Thr Glu Gln Gln Leu Ala Ile Ser Gly Asn Thr Gln Ser 1 5 10 15 Pro Leu Gly His Tyr Ser Leu 20 <210> 147 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 147 His Ser Thr Val Glu Lys Ala Ser Thr Arg Ala Ser Ser Ser Ala Ser 1 5 10 15 Lys Lys Gln Tyr Val 20 <210> 148 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 148 Val Pro Gly Gln Pro Thr Pro Asp Gly Cys Gln Gln Gln Glu Gly Gly 1 5 10 15 Gly Glu Asn Thr Asn Ser Ile 20 <210> 149 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 149 Gly Glu Asn Thr Asn Ser Ile Ser Ser Asn Gly Glu Asp Ser Asp Glu 1 5 10 15 Ala Gln Met Arg Leu Gln Leu 20 <210> 150 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 150 Glu Glu Glu Leu Arg Asn Gln Arg Arg Gln Ala Ser Asn Thr Pro Ser 1 5 10 15 His Ile <210> 151 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 151 Ile Ser Ser Ser Phe Ser Thr Ser Val Tyr Gln Pro Ile Pro Gln Pro 1 5 10 15 Thr Thr Pro Val Ser Phe Thr 20 <210> 152 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 152 Tyr Phe Cys Met Leu Pro Phe Ser Pro Ser Val Asn Gly Arg Ser Tyr 1 5 10 15 Asp Thr Tyr Thr Pro Leu His 20 <210> 153 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 153 Lys Val His Cys Leu Ser Glu Ile Ile Phe Arg Thr Lys Gln Gln Gln 1 5 10 15 Arg Glu Leu Arg Ala His Val 20 <210> 154 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 154 Ser Glu Ser Ile Thr Val Glu Trp Ile Glu Asn Gly Asp Thr Lys Gly 1 5 10 15 Lys Glu Ile Asp Leu Glu Ser 20 <210> 155 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 155 Asn Arg Arg Thr Val Ala Pro Val Lys Asn Glu Thr Pro Ala Lys Asp 1 5 10 15 Asn Arg Val Ala Ala Val Gly 20 <210> 156 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 156 Gln Pro Ile Glu Gln Ser Ala Ser Arg Gln Gln Asn Gly Ser Val Ser 1 5 10 15 Asp Ile Ser <210> 157 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 157 Gln Gln Asn Gly Ser Val Ser Asp Ile Ser Pro Asp Gln Pro Gly Lys 1 5 10 15 Lys Asp Phe Gly Leu Ala Ser 20 <210> 158 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 158 Ser Asn Cys Val Lys Glu Val Glu Lys Leu Gln Glu Lys Arg Glu Arg 1 5 10 15 Arg Arg Leu Gln Gln Gln Glu 20 <210> 159 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 159 Glu Leu Asp Pro Ser Arg Cys Arg Arg Pro Pro Pro His Asp Thr Ser 1 5 10 15 Cys Pro Gln <210> 160 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 160 Ala Pro Gln Arg Asp Asp Leu Lys Leu Leu Cys Glu Gln Asn Glu Glu 1 5 10 15 Glu Val Ser Pro Gln Leu Phe 20 <210> 161 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 161 Leu Gln Glu Glu Glu His Ala Ser Lys Gln Ile Asn Pro Lys Arg Pro 1 5 10 15 Arg Ala Leu <210> 162 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 162 Leu Ile Ile Gln Ser Leu Phe Ala Phe Leu Glu His Arg Lys Met Lys 1 5 10 15 Lys Ser Leu Glu Thr Pro Ile 20 <210> 163 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 163 Phe Ala Ile Ala Thr Ile Asn Lys Ala Gly Trp Gly Thr Ser Gly Arg 1 5 10 15 Lys Thr Ile Val Val Asn Phe 20 <210> 164 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 164 Thr Ile Leu Leu Gly Gly Val Ile Phe Thr Ile Tyr Lys Glu Ser Lys 1 5 10 15 Lys Pro Phe Ser Glu Ser Lys 20 <210> 165 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 165 Phe Thr Ile Tyr Lys Glu Ser Lys Lys Pro Phe Ser Glu Ser Lys Gln 1 5 10 15 Thr Val Leu Ile Val Gly Thr 20 <210> 166 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 166 Tyr Val Val Leu Ile Asn Lys Cys Gly Arg Arg Lys Lys Gly Gln Gln 1 5 10 15 Tyr Asp Met Val Leu Asp Val 20 <210> 167 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 167 His Leu Leu Ile Gln Ser Leu Phe Ala Phe Leu Glu His Arg Arg Met 1 5 10 15 Arg Arg Ala Gly Arg Pro Leu 20 <210> 168 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 168 Arg Arg Ala Gly Arg Pro Leu Lys Leu His Cys Ser Gln Arg Ser Arg 1 5 10 15 Ser Val Ala Leu 20 <210> 169 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 169 Phe Ala Ile Ala Thr Ile Asn Lys Ser Gly Trp Gly Thr Ser Gly Arg 1 5 10 15 Lys Thr Ile Val Val Asn Phe 20 <210> 170 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 170 Leu Tyr Leu Ala Ile Ile Ala Arg Arg Cys Gly Lys Lys Pro Glu Gln 1 5 10 15 Tyr Ser Leu Ala Phe Ala Glu 20 <210> 171 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 171 Phe Ile Ala Ala Leu Asp Asp Ile Thr Val Asp Leu Pro Pro Val Glu 1 5 10 15 Asp Tyr <210> 172 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 172 Leu Gly Asn Asn Thr Ala Cys Gly Thr Trp Val Gly Gln Lys Pro Asp 1 5 10 15 Gly Ser Leu Val Val Asn Ala 20 <210> 173 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 173 Val Val Asn Ala Ala Tyr Asp Gly Cys Tyr Val Arg Glu Glu Asn Gly 1 5 10 15 Asp His Val Met Thr Val Ser 20 <210> 174 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 174 Ser Leu Glu Glu Val Ile Asn Gly His Val Asp Tyr His Lys Gln Glu 1 5 10 15 Leu Lys Cys Pro Ile Met Glu 20 <210> 175 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 175 Ser Asp Gly Pro Val Asp Phe Ile Asp Gly Glu Glu Lys Arg Thr Glu 1 5 10 15 Thr Glu Gly Val Val Gly Phe 20 <210> 176 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 176 Thr Val Thr Phe Ile Gly Leu Trp Ile His Tyr Arg Asn Arg Ser Pro 1 5 10 15 Lys Thr His Asp Val Asn Ala 20 <210> 177 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 177 Val Phe Pro Gln Ile Leu Arg Ala Leu Glu Glu Asp Pro Glu Asp Asn 1 5 10 15 His Arg Leu Phe Ala Gln Asn 20 <210> 178 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 178 Phe Ala Gln Asn Pro Val Asp Val Thr Ser Leu Arg Pro Ser Asp Leu 1 5 10 15 Glu Glu Phe Val Lys Gly 20 <210> 179 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 179 Leu Leu Pro Asn Val Asp Ser Ile Ser Arg Ser Val Gln Asp Gln Glu 1 5 10 15 Asp Asp Val Pro Ile Ile Asp 20 <210> 180 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 180 His Ile Ser Ser Val Glu Thr Thr Lys Val Ala Ala Arg Gly Arg Lys 1 5 10 15 Lys Gln Ile Ile Gln Ser Trp 20 <210> 181 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 181 Gly Arg Lys Lys Gln Ile Ile Gln Ser Trp Asn Trp Glu Pro Gln Arg 1 5 10 15 Gly Arg Met Leu Asn Leu Ile 20 <210> 182 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 182 Glu Phe Glu Glu Lys Ile Asn Lys Phe His Met Glu Lys Lys Glu Gln 1 5 10 15 Glu Glu Thr Ala Arg Asn Ala 20 <210> 183 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 183 Pro Ser Met Glu Glu Ser Gly Asp Asn Leu Val Glu Thr Glu Ser Glu 1 5 10 15 Glu Glu Pro Ser Asp Ser Glu 20 <210> 184 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 184 Leu Val Glu Thr Glu Ser Glu Glu Glu Pro Ser Asp Ser Glu Glu Glu 1 5 10 15 Pro Asp Ser Ala Gln Cys Gly 20 <210> 185 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 185 Leu Asn Gln Lys Thr Ser Gly Asp Asn Leu Ile Glu Thr Glu Pro Glu 1 5 10 15 Glu Glu Gln Ser Asp Ser Glu 20 <210> 186 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 186 Thr Ser Gly Asp Asn Leu Ile Glu Thr Glu Pro Glu Glu Glu Gln Ser 1 5 10 15 Asp Ser Glu Pro Asp Ser Ala 20 <210> 187 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 187 Leu Asn Arg Lys Thr Ser Gly Asp Asn Leu Ile Glu Thr Glu Ser Glu 1 5 10 15 Glu Glu Gln Ser Asp Ser Glu 20 <210> 188 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 188 Glu Glu Gln Ser Asp Ser Glu Glu Glu Pro Ser Asp Ser Glu Glu Glu 1 5 10 15 Pro Asp Ser Ala Gln Cys Gly 20 <210> 189 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 189 Gln Cys Gly Thr Thr Asn Pro Arg Ser Leu Asn Gln Lys Thr Ser Gly 1 5 10 15 Gly Glu Glu Gly Glu Ser Glu 20 <210> 190 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 190 Ser Gly Gly Glu Glu Gly Glu Ser Glu Ser Lys Ser Thr Glu Ser Ser 1 5 10 15 Ser Ser Ile Arg Arg Asn Leu 20 <210> 191 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 191 Pro Phe Asn Gly Ala Gly Asn Arg Met Pro Pro Gln Glu Tyr Thr Asn 1 5 10 15 Pro Pro Ser Ser Asn Gln Asp 20 <210> 192 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 192 Met Pro Pro Gln Glu Tyr Thr Asn Pro Pro Ser Ser Asn Gln Asp Ile 1 5 10 15 Asp Leu Pro Ala Tyr Asn Lys 20 <210> 193 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 193 Gly Ile Ile Ser Asn Met Asp Ala Val Lys Ser Asn His Gln Thr Ser 1 5 10 15 Ser Lys Gln Gln Tyr Val 20 <210> 194 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 194 Thr Gln Ser Met Asp Glu Ile Gly Leu Ser Pro Arg Lys Asp Ser Tyr 1 5 10 15 Gln Ile Phe Pro Asp Pro Ser 20 <210> 195 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 195 Val Asp Glu Asp Lys Glu Gly Thr Cys Asp Gln Thr Lys Lys Glu Asn 1 5 10 15 Glu Gln <210> 196 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 196 Ile Asn Pro His Leu Ser Ile Gln Val Ser Ser Lys Asp Asn Glu Ser 1 5 10 15 Trp Asp Glu Gly Ile Glu Ser 20 <210> 197 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 197 His Asn Gly Thr Ile Leu Gly Glu Ala Glu Lys Glu Asp Asp Glu Ser 1 5 10 15 Gly Asn Ile Asp His Gln Leu 20 <210> 198 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 198 Arg Pro Glu Ile Gly Leu Met Gln Pro Ser Ala Pro Pro Asp Tyr Glu 1 5 10 15 His Glu Leu Ile Asn Pro His 20 <210> 199 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 199 Pro Gln Arg Arg Val Gln Ala Gln Leu Ser Pro His Tyr Gln Arg Arg 1 5 10 15 Arg Leu Pro Pro Pro Leu Glu 20 <210> 200 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 200 Pro Leu Glu Asp Gln Ile Leu Leu Glu Trp Glu Glu Arg Lys Arg Lys 1 5 10 15 Gln Pro Trp Val Phe Asn Gly 20 <210> 201 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 201 Phe Arg Leu His Ser Val Lys Ala Asp Leu Asn Gln Arg Glu Lys Ile 1 5 10 15 Asn Arg Glu Asn Thr Leu Gly 20 <210> 202 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 202 Gly Glu Ile Gly Asp His Ile Arg Gln Glu Ala Asp Met Asp Glu Gln 1 5 10 15 Gly Gly Leu Thr Leu Tyr Leu 20 <210> 203 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 203 Asn Trp Ser Gly Asn Ala Lys Ala Leu Gln Glu Arg Gly Gly Lys Glu 1 5 10 15 His Gly Asp Pro Glu Ala Tyr 20 <210> 204 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 204 Gln Cys Ser Asp Gly Arg Phe Val Leu Leu Arg Arg Ser Asn Arg Val 1 5 10 15 Gly Glu Ala Pro Gly Gln Leu 20 <210> 205 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 205 Ser Ala Glu Phe Tyr Val Arg Cys Ser Phe Ser Ser Glu Glu Val Arg 1 5 10 15 Glu Arg Tyr Leu Gln Gly Gly 20 <210> 206 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 206 Val Arg Glu Arg Tyr Leu Gln Gly Gly Pro Glu Ala Ser Glu Ser Thr 1 5 10 15 Asp Ile Met Phe Ile Asp Arg 20 <210> 207 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 207 Met Val Arg Asn Thr His Leu Asn Leu Gln Pro Gln Glu Arg Gln Ala 1 5 10 15 Phe Tyr Arg Leu Leu Glu 20 <210> 208 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 208 Val Ala Leu Tyr Leu Ala Asn Asp Met Glu Glu Asp Glu Glu Asp Tyr 1 5 10 15 Lys Tyr Glu Ile Phe Pro Trp 20 <210> 209 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 209 Val Ser Arg Arg Cys Cys Asp Glu Val Met Ser Lys Asp Pro Thr His 1 5 10 15 Trp Ala Trp Leu Arg Asp Arg 20 <210> 210 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 210 His His Ser Gly Ala Met Arg Gly Tyr Leu Arg Asn Glu Asp Asp Phe 1 5 10 15 Ser Pro Arg Gly Pro Gly Leu 20 <210> 211 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 211 Gly Val Cys Asp Ser Gly Gly Val Ser His Asn Asn Ser Ser Ser Pro 1 5 10 15 Glu Gln Glu Ile Phe His Tyr 20 <210> 212 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 212 Ser Ser Pro Glu Gln Glu Ile Phe His Tyr Thr Asn Arg Glu Trp Ser 1 5 10 15 Gln Glu Leu Leu Met Leu Ser 20 <210> 213 <211> 17 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 213 Met Gln Cys Asp Ile Gln Thr Ser Arg Arg Glu Cys Pro Ile Glu Pro 1 5 10 15 Thr <210> 214 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 214 Leu Pro Ala Ser Asn Ser Ala Val Leu Asn Pro Asp Gly Ser Asn Gln 1 5 10 15 Ser Gly Phe Ala Gln Gly Gly 20 <210> 215 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 215 Pro Tyr Asn Ser Ala Gly Asn Pro Tyr Pro Pro Ala Gly Gln Ser Ser 1 5 10 15 Pro Ala Gly Asn Gln Ser Ile 20 <210> 216 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 216 Pro Ala Gly Asn Gln Ser Ile Val Phe Gln Asn Ser Asn Gln Pro Gly 1 5 10 15 Ser Asn Thr Pro Gln Tyr Thr 20 <210> 217 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 217 Gln Asn Ser Asn Gln Pro Gly Ser Asn Thr Pro Gln Tyr Thr Ser Ser 1 5 10 15 Pro Ala Pro Ser Gly Ser Ser 20 <210> 218 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 218 Pro Gly Pro Val Gly Ala Gln Asn Ile Pro Gly Asn Tyr Pro Gln Ser 1 5 10 15 Ala Thr Ala Gly Asn Phe Asn 20 <210> 219 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 219 Ser Pro Ser Ser Gly Leu Gly Gln Phe Ser Arg Pro Ala Ser Ser Gly 1 5 10 15 Thr Pro Phe Asn Ser Gly Gln 20 <210> 220 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 220 His Gly Trp Asn Ala Ile Ser Asp Gly Lys Ser His Lys Arg Lys Gln 1 5 10 15 Pro Leu Pro Lys Arg Met Phe 20 <210> 221 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 221 Leu Ala Thr Ser Phe Leu Gln His Pro Trp Glu Glu Asn Arg Asn Asp 1 5 10 15 Tyr Leu Ser Asn Ser Ala Asn 20 <210> 222 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 222 Tyr Ser Leu Arg Lys Leu Ser Lys Ile Leu Ser Thr Thr Lys Glu Glu 1 5 10 15 Asp Ala Ala Cys Ser Ala Ser 20 <210> 223 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 223 Pro Arg Asp Ser Thr Val Thr Asn Gly Asp Asp Lys Gln Ser Ser Lys 1 5 10 15 Asn Ala Val Gln Gly Lys Arg 20 <210> 224 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 224 Ala Leu Gln Leu Ser Asn Ser Asp Gly Lys Pro Glu Pro Lys Ser Ser 1 5 10 15 Glu Ala Leu Glu Asn Met Phe 20 <210> 225 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 225 Val Lys Gly Asn Ser Asn Asn Asn Asn Asn Tyr Arg Arg Arg Asn Arg 1 5 10 15 Arg Gly Gly Arg Gln Ser Val 20 <210> 226 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 226 Thr Pro Ser Pro Asn Lys Pro Arg Met His Arg Lys Arg Asn Ser Arg 1 5 10 15 Gln Ser Leu Gly Ala Thr Pro 20 <210> 227 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 227 Leu Leu Leu Leu Phe Ala Phe Phe Ala Thr Tyr Glu Ala Lys Glu Tyr 1 5 10 15 Glu Arg Ile Ile Lys Glu Leu 20 <210> 228 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 228 Gly Val Val Leu Phe Asn Arg Thr Asp Thr Glu Val Gln Ser Glu Gln 1 5 10 15 Pro Ile Pro Pro Arg His Pro 20 <210> 229 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 229 Val Phe Pro Asp Ser Pro Ile Ala Lys Tyr Arg Pro Pro Gln Ser Pro 1 5 10 15 Ala Arg Pro Leu Arg Asn Asp 20 <210> 230 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 230 Pro Gln Ser Pro Ala Arg Pro Leu Arg Asn Asp Thr Lys Glu His Asn 1 5 10 15 Pro Cys Ala Lys Asp Glu Ser 20 <210> 231 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 231 Arg Ala Asp Asp Thr Trp Gln Leu Ile Val Asn Asn Asp Glu Tyr Lys 1 5 10 15 Gln Ala Ile Gln Ile Glu Glu 20 <210> 232 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 232 Phe Pro Gln Ser Tyr Asn Pro Ile Cys Lys Gln His Tyr Thr Gln Gln 1 5 10 15 Thr Leu Ala Ser Ile Lys Ser 20 <210> 233 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 233 Val Leu Leu Leu Leu Phe Ala Phe Phe Ala Thr Asp Glu Ser Gln His 1 5 10 15 Leu Arg Asn Phe Cys Thr Asn 20 <210> 234 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 234 Asn Asp Leu Gln Pro Thr Asp Val Ser Ser Arg Val Gly Gly Ser Asp 1 5 10 15 Glu Arg Phe Leu Cys Arg Ser 20 <210> 235 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 235 Asn Ile Asn Ser Asn Ser Ser His Ser Ser Asn Asn Asn Asn Asn Gly 1 5 10 15 Ser Ser Ser Asn Asn Asp Asn 20 <210> 236 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 236 Asn Asn Asn Asn Gly Ser Ser Ser Asn Asn Asp Asn Asn Ser Asn Asp 1 5 10 15 Asp Ala Ala Ser Ser Ser Asn 20 <210> 237 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 237 Ala Ala Ser Ser Ser Asn Ser Lys Asn Asn Asn Thr Ser Asn Glu Ser 1 5 10 15 Ser His Ser Asn Asn Asn Thr 20 <210> 238 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 238 Ser Arg Thr Thr Thr Pro Thr Thr Thr Thr Thr Pro Ser Ser Ser Ser 1 5 10 15 Ser Thr Ala Ser Asn Gly Phe 20 <210> 239 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 239 Asp Gly Lys Phe Ile Leu Glu Leu Ala Arg Ser Lys Asp Gly Asp Lys 1 5 10 15 Ser Gly Trp Val Ser Val Thr 20 <210> 240 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 240 Thr Pro Thr Ser Ala Val Thr Thr Ala Tyr Pro Lys Asn Glu Asn Ser 1 5 10 15 Thr Ser Leu Ser Phe Ser Asp 20 <210> 241 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 241 Gln Ser Ser Pro Trp Gln Arg Asp Gln Pro Trp Lys Gln Ser Arg Pro 1 5 10 15 Arg Arg Gly Ile Ser Lys Glu 20 <210> 242 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 242 Thr Leu Gly Arg Ala Ala Leu Arg Thr Ala Ala Arg Lys Arg Arg Arg 1 5 10 15 Pro His Glu Pro Leu Thr Thr 20 <210> 243 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 243 Thr Ile Lys Gly Glu Asp Asp Ala Glu Arg Leu Glu Lys Glu Pro Lys 1 5 10 15 Lys Ala Val Ser Asp Asp Ser 20 <210> 244 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 244 Ser Lys Glu Ala Ser Pro Gly Gln Gln Val Glu Pro Gln Pro Lys Asp 1 5 10 15 Glu Thr Val Asp Val Glu Met 20 <210> 245 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 245 Leu Asn Gly Asp Leu Lys Ala Ser Ile Gly Lys Pro Lys Ser Lys Pro 1 5 10 15 Lys Pro Lys Ala Lys Leu Ser 20 <210> 246 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 246 Leu Ala Pro Pro Gln Gly Lys Pro Ser Pro Ser Pro Gly Ser Ser Ser 1 5 10 15 Ser Ser Thr Ser Pro Ala Thr 20 <210> 247 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 247 Gly Gly Gly Lys Ser Pro Ser His Thr Gly Thr Lys Lys Arg Ser Pro 1 5 10 15 Pro Tyr Ser Ala Gly Ser Pro 20 <210> 248 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 248 Pro Ser Pro Gly Asp Arg Ser Ala Ser Pro Pro Glu Arg Arg His Met 1 5 10 15 Gln Gln Gln Pro His Leu Gln 20 <210> 249 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 249 Ser Ser Ser Ala Met Phe His Thr Ser Ser Leu Arg Asn Glu Gln Ser 1 5 10 15 Ser Asp Leu Pro Leu Asn Leu 20 <210> 250 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 250 Ser Val Ala Glu Cys Asn Arg Gln Thr Thr Met Thr Asp Glu His Lys 1 5 10 15 Ser Asn Ile Asn Ser Asn Ser 20 <210> 251 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 251 Ser Asn Ser Ser His Ser Ser Asn Asn Asn Asn Asn Gly Ser Ser Ser 1 5 10 15 Asn Asn Asp Asn Asn Ser Asn 20 <210> 252 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 252 Asn Ser Asn Asp Asp Ala Ala Ser Ser Ser Asn Ser Lys Asn Asn Asn 1 5 10 15 Thr Ser Asn Glu Ser Ser His 20 <210> 253 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 253 Leu Ser Arg Thr Thr Thr Pro Thr Thr Thr Thr Thr Pro Ser Ser Ser 1 5 10 15 Ser Ser Thr Ala Ser Asn Gly 20 <210> 254 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 254 Thr Val Thr Pro Thr Ser Ala Val Thr Thr Ala Tyr Pro Lys Asn Glu 1 5 10 15 Asn Ser Thr Ser Leu Ser Phe 20 <210> 255 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 255 Gln Ser Ser Pro Trp Gln Arg Asp Gln Pro Trp Lys Gln Ser Arg Pro 1 5 10 15 Arg Arg Gly Ile Ser Lys Glu 20 <210> 256 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 256 Arg Gly Ile Ser Lys Glu Leu Ser Leu Phe Phe His Arg Pro Arg Asn 1 5 10 15 Ser Thr Leu Gly Arg Ala Ala 20 <210> 257 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 257 Leu Gly Arg Ala Ala Leu Arg Thr Ala Ala Arg Lys Arg Arg Arg Pro 1 5 10 15 His Glu Pro Leu Thr Thr Ser 20 <210> 258 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 258 Thr Ile Lys Gly Glu Asp Asp Ala Glu Arg Leu Glu Lys Glu Pro Lys 1 5 10 15 Lys Ala Val Ser Asp Asp Ser 20 <210> 259 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 259 Glu Ser Lys Glu Ala Ser Pro Gly Gln Gln Val Glu Pro Gln Pro Lys 1 5 10 15 Asp Glu Thr Val Asp Val Glu 20 <210> 260 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 260 Asp Leu Asn Gly Asp Leu Lys Ala Ser Ile Gly Lys Pro Lys Ser Lys 1 5 10 15 Pro Lys Pro Lys Ala Lys Leu 20 <210> 261 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 261 Ala Pro Pro Gln Gly Lys Pro Ser Pro Ser Pro Gly Ser Ser Ser Ser 1 5 10 15 Ser Thr Ser Pro Ala Thr Leu 20 <210> 262 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 262 Asn Ser Ser Tyr Ser Ile His Ser Leu Leu Gly Gly Ser Ser Gly Ser 1 5 10 15 Gly Ser Ser Ser Phe Ser Ser 20 <210> 263 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 263 Gly Gly Lys Ser Pro Ser His Thr Gly Thr Lys Lys Arg Ser Pro Pro 1 5 10 15 Tyr Ser Ala Gly Ser Pro Val 20 <210> 264 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 264 Ser Pro Gly Asp Arg Ser Ala Ser Pro Pro Glu Arg Arg His Met Gln 1 5 10 15 Gln Gln Pro His Leu Gln Arg 20 <210> 265 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 265 Ser Ser Ala Met Phe His Thr Ser Ser Leu Arg Asn Glu Gln Ser Ser 1 5 10 15 Asp Leu Pro Leu Asn Leu Ser 20 <210> 266 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 266 Arg Val Thr Thr Cys Phe Gln Gln Leu Arg Asp Lys Leu Gln Ser Ser 1 5 10 15 Gly Ser Phe Arg Lys Ser Ser 20 <210> 267 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 267 Ser Ala Cys Gly Glu Arg Phe Arg Lys Ile Phe Lys Ser Ala Arg Lys 1 5 10 15 Thr Glu Leu Pro Glu Leu Trp 20 <210> 268 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 268 Trp Lys Val Pro Leu Val Ala His Glu Leu Thr Ser Arg Gln Ser Ser 1 5 10 15 Gln Gln Leu Gln Val Val Ala 20 <210> 269 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 269 Gln Ile Ser Thr Lys Glu Ile Thr Tyr Asn Ser Asn Ser Asn Thr Ser 1 5 10 15 Glu Asn Asn Met Thr Ile Ile 20 <210> 270 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 270 Lys Asn Gly His Leu Leu Asp Met Val Leu Asn Gln Lys Glu Arg Thr 1 5 10 15 Ser Asp Tyr Ser Ser Gly Ala 20 <210> 271 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 271 Ala Pro Ser Leu Glu Asn Ile Pro Arg Ala Pro Pro Arg Tyr Trp Lys 1 5 10 15 Asn Pro Phe Lys Arg Arg Ala 20 <210> 272 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 272 Leu Asn Thr Ser Pro Arg Thr Val Pro Lys Ile Thr Asp Glu Lys Thr 1 5 10 15 Lys Asp Ile Ala Thr Arg Pro 20 <210> 273 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 273 Pro Lys Met Pro Val Pro Ile Asn Ile Tyr Ser Pro Asp Ala Pro Glu 1 5 10 15 Glu Pro Ile Asn Leu Ala Pro 20 <210> 274 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 274 Pro Pro Gly His Gln Pro Ser Cys Arg Thr Gln Ser Gln Lys Thr Glu 1 5 10 15 Pro Thr Glu Asn Arg His Leu 20 <210> 275 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 275 Val His Pro Phe Asn Gly Met Asn Ile Met Lys Arg Arg His Glu Met 1 5 10 15 Thr Pro Thr Pro Thr Ile Leu 20 <210> 276 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 276 Asn Pro Ser Leu Val Ile Asp Val Tyr Gly Thr Arg Arg Gly Pro Lys 1 5 10 15 Ser Lys Asn Gly Gly Pro Ile 20 <210> 277 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 277 Pro Lys Ser Lys Asn Gly Gly Pro Ile Ala Ser Gly Gly Ser Gly Ser 1 5 10 15 Gly Ser Arg Lys Ser Asn Val 20 <210> 278 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 278 Ser Arg Lys Ser Asn Val Ala Ala Val Ala Pro Gln Gln Gln Gln Ser 1 5 10 15 Gln Pro Ala Gln Pro Val Ala 20 <210> 279 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 279 Gln Leu Ser Pro Arg Met Arg Glu Gln Leu Gln Gln Gln Asn Gln Gln 1 5 10 15 Gln Ser Asp Asn Glu Glu Glu 20 <210> 280 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 280 Gln Gln Gln Ser Asp Asn Glu Glu Glu Glu Gln Asp Asp Glu Tyr Glu 1 5 10 15 Arg Lys Ser Val Asp Ser Ala 20 <210> 281 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 281 Ala Met Asp Leu Ser Gln Gly Thr Pro Val Lys Glu Asp Glu Gln Gln 1 5 10 15 Gln Gln Pro Gln Gln Pro Leu 20 <210> 282 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 282 Ser Ser Asn Ala Ser Ser Asn Ser Asn Gly Asn Ser Ser Ser Asn Ser 1 5 10 15 Ser Ser Asn Gly Thr Thr Ser 20 <210> 283 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 283 Met Thr Ser Lys Lys Arg Lys Thr Leu Leu Asp Ala Asp Asp 1 5 10 <210> 284 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 284 Leu Asp Ala Asp Asp Asp Asn Asp Asn Phe Asp Glu Asp Asp Ser Gly 1 5 10 15 Ser Asp Phe Asp Asp Asp Glu 20 <210> 285 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 285 Asn Phe Asp Glu Asp Asp Ser Gly Ser Asp Phe Asp Asp Asp Glu Asp 1 5 10 15 Pro Asp Gln Ile Glu Val Pro 20 <210> 286 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 286 Asn Asn Asn Asn Asn Asn Ile Val Thr Ser Val Asn Asn Arg Asn Asn 1 5 10 15 Asn Asn Ile Ile Ser Thr Asn 20 <210> 287 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 287 Asn Asn Arg Asn Asn Asn Asn Ile Ile Ser Thr Asn Gly Ser Ser Asn 1 5 10 15 Asn Asn Asn Asn Ser Ile Asn 20 <210> 288 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 288 Ala Ala Ala Ala Ala Ala Ala Ala Ala Leu Gln Gln Gln Gln Gln Gln 1 5 10 15 Gln Ser Pro Leu His Ser Pro 20 <210> 289 <211> 25 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 289 Ser Asn Asn Ser His Gln Asp Glu Glu Glu Leu Asp Asp Glu Glu Glu 1 5 10 15 Asp Glu Glu Glu Asp Glu Asp Glu Asp 20 25 <210> 290 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 290 Glu Leu Asp Asp Glu Glu Glu Asp Glu Glu Glu Asp Glu Asp Glu Asp 1 5 10 15 Asp Glu Glu Glu Asn Ala Ser 20 <210> 291 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 291 Gln Glu Thr Arg Thr Glu Pro Ser Ala Thr Thr Gln Gln Gln His Gln 1 5 10 15 Gln Gln Asp Thr Glu Asp Leu 20 <210> 292 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 292 Thr Ala Thr Ser Asn Thr Asn Asn Asn Asn Asn Asn Asn Ser Ser Ser 1 5 10 15 Gly Asn Ser Glu Lys Arg Lys 20 <210> 293 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 293 Asn Asn Asn Asn Ser Ser Ser Gly Asn Ser Glu Lys Arg Lys Lys Lys 1 5 10 15 Asn Asn Asn Asn Asn Asn Gly 20 <210> 294 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 294 Arg Arg Ala Asp Gly Ala Ala Ser Pro Thr Pro Ser Asp Ser Ser Asp 1 5 10 15 Ser Asp Ile Ser Leu Gly Thr 20 <210> 295 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 295 Ser Pro Val Pro Ser Ser Leu Gln Leu Gln His Ser Pro Gly Ser Thr 1 5 10 15 Ser Asn Gly Ala Asn Asp Arg 20 <210> 296 <211> 24 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 296 Gly Ala Asn Asp Arg Glu Glu Ser Leu Ser Val Asp Asp Asp Lys Pro 1 5 10 15 Arg Asp Leu Ser Gly Ser Leu Pro 20 <210> 297 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 297 Asn Ser Thr Ala Val Ala Ala Ala Ala Ser Val Gly Gly Gly Gly Gly 1 5 10 15 Asn Gln His His Glu Pro Thr 20 <210> 298 <211> 17 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 298 Met Ala Asp Val Ala Glu Gln Lys Asn Glu Thr Pro Val Val Glu Lys 1 5 10 15 Val <210> 299 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 299 Glu Pro Thr Val Ser Phe Ala Ala Asp Lys Asp Glu Lys Lys Asp Glu 1 5 10 15 Asp Lys Lys Glu Asp Ser Ala 20 <210> 300 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 300 Lys Lys Glu Asp Ser Ala Ala Asp Gly Glu Asp Thr Lys Lys Glu Ser 1 5 10 15 Ser Glu Ala Val Leu Pro Ala 20 <210> 301 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 301 Lys Glu Ser Ser Glu Ala Val Leu Pro Ala Val Glu Asn Gly Ser Glu 1 5 10 15 Glu Val Thr Asn Gly Asp Ser 20 <210> 302 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 302 Cys Phe Gly Gly Lys Ser Ile Lys Thr Pro Thr Asn Glu Thr Ser Asp 1 5 10 15 Thr Glu Ser Ala Asp Ile Lys 20 <210> 303 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 303 Val Ser Ala Glu Glu Glu Glu Thr Val Asn Glu Gln Glu Ser Pro Gln 1 5 10 15 Glu Gln Gln Gln Gln Gln Gln 20 <210> 304 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 304 Thr Val Asn Glu Gln Glu Ser Pro Gln Glu Gln Gln Gln Gln Gln Gln 1 5 10 15 Lys Lys Ala Lys Arg Leu Pro 20 <210> 305 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 305 Ala Ile Leu Arg Arg Phe Asn Glu Lys Asp Ser Arg Lys Tyr Lys Asp 1 5 10 15 Asn Gln Glu Ser Leu Asn Ile 20 <210> 306 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 306 Gln Glu Ile Pro Ala Thr Asn Asn Ala Ile Asp Gly Asn Asp Asn Glu 1 5 10 15 Pro Lys Ala Ile Val Trp Gln 20 <210> 307 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 307 Asp Glu Pro Leu Asn Asn Thr Tyr Ile Glu Ser Pro Pro Glu Ser Ser 1 5 10 15 Asp Arg Glu Val Ser Pro Lys 20 <210> 308 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 308 Pro Lys Leu Val Val Gly Thr Lys Lys Tyr Gly Arg Arg Ser Arg Pro 1 5 10 15 Gln Gln Gly Ile Tyr Glu Leu 20 <210> 309 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 309 Gln Gln Gly Ile Tyr Glu Leu Ser Val Thr Asp Ser Asp Asn Thr Asp 1 5 10 15 Glu Asp Gln Leu Gln Gln Gln 20 <210> 310 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 310 Asp Ser Asp Asn Thr Asp Glu Asp Gln Leu Gln Gln Gln Gln Asn Gln 1 5 10 15 Arg Ser Leu Asn Gly Cys Asp 20 <210> 311 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 311 Asn Gly Cys Asp Glu Leu Gly Val Gln Val Gln Arg Ser Ser Ser Gln 1 5 10 15 Ser Asp Leu Pro Gly Ser Arg 20 <210> 312 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 312 Ser Lys Leu Lys Arg Cys Ala Ser Leu Pro Ala Arg Arg Lys Met Asn 1 5 10 15 Ser Asn Thr Thr Gly Ala Thr 20 <210> 313 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 313 Pro Gln Leu Gln Arg Ile Gln Pro Tyr Leu Val Pro Gln Arg Asn Arg 1 5 10 15 Ser Gln Ala Gly Gly Leu Leu 20 <210> 314 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 314 Pro Ser Leu Leu Ser Ser Ala Leu Met Pro Lys Arg Lys Arg Gly Arg 1 5 10 15 Pro Arg Lys Leu Ser Gly Ser 20 <210> 315 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 315 Gly Lys Met Cys Asn Glu Ser Tyr Ser Gly Asn Asp Asp Gly Ser Asp 1 5 10 15 Asp Asn Gln Pro Asn Ala Gly 20 <210> 316 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 316 Asp Leu Asn Glu Ser Arg Asp Ser Leu Pro Ser Lys Arg Ser Lys Asn 1 5 10 15 Ser Lys Asp His Arg Val Val 20 <210> 317 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 317 Lys Asn Ser Lys Asp His Arg Val Val Ser His His Glu Asp Asn Ser 1 5 10 15 Thr Ser Asp Gly Asn Asp Ser 20 <210> 318 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 318 Val Val Ser His His Glu Asp Asn Ser Thr Ser Asp Gly Asn Asp Ser 1 5 10 15 Asp Gly Glu Gly Leu Asp Thr 20 <210> 319 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 319 Ala Gly Ser Phe Ser Thr Ala Thr Val Lys Pro Arg Val Gln Lys Arg 1 5 10 15 Pro Lys Leu Gly Lys Gln Asn 20 <210> 320 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 320 Ser Leu Pro Ala Thr Ser Asn Ala Leu Ser Thr Ser Ser Ser Ser Ser 1 5 10 15 Thr Ser Ser Ser Ser Gly Ser 20 <210> 321 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 321 Ser Leu Val Gly Gln Glu His Ile Val Met Pro Glu Ser Ser Asp Ser 1 5 10 15 Asn Thr Leu Cys Pro Ile Ser 20 <210> 322 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 322 Glu Pro Leu Thr Asn Ser Asp Gly Cys Ser Glu Asp Asn Asn Lys Glu 1 5 10 15 Pro Glu Lys Ser Asn Ser Ser 20 <210> 323 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 323 Cys Ser Glu Asp Asn Asn Lys Glu Pro Glu Lys Ser Asn Ser Ser Gln 1 5 10 15 Ser Ser Asp His Ile Ala Ser 20 <210> 324 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 324 Pro Ser Gly Gly Ser Ser Leu Leu Asn Ile Asn Pro Ser Glu Asn Arg 1 5 10 15 Ser Asp Val Glu Leu Asn Leu 20 <210> 325 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 325 Gly Ser Gly Leu Ile Asn Ser Ser Tyr Tyr Tyr Glu Ser Gly Gln Thr 1 5 10 15 Gln Ser Pro Leu Thr His Asp 20 <210> 326 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 326 Asn Leu Asn Gly Asp Gly Ser Ala Ala Gln Ser Thr Ser Gln Ser Gln 1 5 10 15 Ser Gln Ser Pro Ala Ala Thr 20 <210> 327 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 327 Asp Ala Gly Val Pro Pro Leu Pro Leu Pro Leu Pro Gln Ser Thr Pro 1 5 10 15 Gln Pro Leu Leu Met Ala Leu 20 <210> 328 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 328 Ala Met Gly Val Ile Pro Ser Thr Asn Asp Ser Asn Asn Asn Asn Asn 1 5 10 15 Asn Asn Asn Asn Val Asp Asn 20 <210> 329 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 329 Asn Asn Asn Asn Asn Asn Asn Asn Asn Val Asp Asn Thr Ser Asp Glu 1 5 10 15 Ser Asn Pro Val Thr Ile Tyr 20 <210> 330 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 330 Leu Glu Leu Gln Arg Lys Gln Leu Glu Ala Ser Ala Gln Glu Glu Ala 1 5 10 15 Asp Gln Leu His Pro Asp Thr 20 <210> 331 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 331 Ala Gln Glu Glu Ala Asp Gln Leu His Pro Asp Thr Asp Pro Asn Pro 1 5 10 15 Asp Ser Gly Gly Gln Leu Pro 20 <210> 332 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 332 Glu Thr Val Thr His Thr Gly Glu Pro Pro Pro Asp Pro Ser Ser Ser 1 5 10 15 Ser Thr Pro Asp Ser Thr Thr 20 <210> 333 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 333 Ser Ser Ser Thr Pro Asp Ser Thr Thr Pro Ser Pro Asn Asp Lys Glu 1 5 10 15 Thr Glu Ile Gln Met Leu Pro 20 <210> 334 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 334 Ala Ala Val Phe Met Leu Arg Asn Val Pro Asp Tyr Arg Arg Lys Gln 1 5 10 15 Gln Gln Leu His Leu His Lys 20 <210> 335 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 335 Thr Thr Ser Ala Gly Asn Arg Arg Gln His Arg Tyr Arg Pro Arg Lys 1 5 10 15 Leu Ile Lys Ser Asp 20 <210> 336 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 336 Gln His Arg Tyr Arg Pro Arg Lys Leu Ile Lys Ser Asp Asp Glu Asp 1 5 10 15 Asp Asp Ser Asn Ser Glu Lys 20 <210> 337 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 337 Ser His His His Gln Ser Gln Ser Ala Arg Tyr His Gln Lys Asn Asn 1 5 10 15 Ser Arg Ala Glu Gln Cys Cys 20 <210> 338 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 338 Ala Thr Gln Arg Val Lys Met Leu Arg Arg Ala Arg Lys Gln Lys Tyr 1 5 10 15 Gln Asp His Cys Leu Glu Thr 20 <210> 339 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 339 Ser Leu Thr Pro His Gln His Gln Gln Gln Asn Gln Gln Gln Gln Gln 1 5 10 15 Gln Gln Arg Lys Ser Ala Glu 20 <210> 340 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 340 Pro Ile Arg Gln Pro Ala Ala Gln Gln Gln Pro Gln Gln Gln Gln Arg 1 5 10 15 Gln Arg Cys Asn Thr Gly Ser 20 <210> 341 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 341 Lys Asn Leu Leu Gln Gly Tyr Gln Arg Leu Glu Gln Ser Gln Gln Gln 1 5 10 15 Gln Gln Gln Gln Arg Ser Ser 20 <210> 342 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 342 Tyr Ala Lys Leu Ser Glu Gln Leu Gln Gln Lys Asp Arg Glu Gln Arg 1 5 10 15 Arg Gln Arg His Lys Gln 20 <210> 343 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 343 Gln Met Leu Gln Asp Gln Pro Lys Asp Ala Ser Arg Ser Glu Gln Arg 1 5 10 15 Pro Pro Thr Ser Asn Ser Ser 20 <210> 344 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 344 Phe Arg Gly Ser Pro Leu Lys Val Lys Arg Ser Asp 1 5 10 <110> YEUNGNAM EDUCATIONAL FOUNDATION <120> PREPARATION METHOD FOR BRAND MEAT AND IDENTIFICATION METHOD FOR          SAME <130> dpp20042285kr <160> 344 <170> KopatentIn 1.71 <210> 1 <211> 23 <212> PRT <213> Artificial Sequence <220> <0002> K000-1 peptide <400> 1 Lys Ser Ile Ala Ser Ser Ser Ser Trp Met His Ser Asn Gly Asp Glu   1 5 10 15 Gly Ser Ser Arg Pro Gly Val              20 <210> 2 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K000-2 peptide <400> 2 Arg Val Leu Phe Pro Asp Asn Met Lys Asp Ser Ser Thr Ser Ser Gly   1 5 10 15 Glu Gln Gly Arg Asn Met Val              20 <210> 3 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K000-3 peptide <400> 3 Phe Ile Asp Thr Tyr Gly His Val Thr Ser His Gly Asn Asp Asp Asn   1 5 10 15 Gly Gly Phe Pro Glu Gln Gln              20 <210> 4 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K000-4 peptide <400> 4 Lys Arg His Arg Asp Leu Leu Ser Pro Val Leu Asp Arg Arg Lys Asp   1 5 10 15 Lys Lys Leu Lys Arg Ala Ala              20 <210> 5 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K000-5 peptide <400> 5 Glu Gly Asp Asp Cys Met Thr Ser Arg Pro Ser Glu Ser Pro Glu Asp   1 5 10 15 Lys Asn Ile Cys Ala Ser Pro              20 <210> 6 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K001-1 peptide <400> 6 Ala Leu Arg Glu Gln Leu Asp Lys Phe Asn Lys Gln Gln Glu Lys Cys   1 5 10 15 Gln Ser Thr Leu Ser Ser Ile              20 <210> 7 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> K001-2 peptide <400> 7 Thr Ala Leu Ser Arg Ser Tyr Val Pro Ala Ala Thr Thr Ser Gln Lys   1 5 10 15 Pro Asn Val Phe Arg Gly              20 <210> 8 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K001-3 peptide <400> 8 Gln Leu His Gly Val Ser Asn Lys Pro Lys Asp Lys Lys Lys Lys Lys   1 5 10 15 Lys Glu Ile Thr Asn Arg Thr              20 <210> 9 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K002-1 peptide <400> 9 Ala Lys Thr Phe Tyr Asp Asn Tyr Thr Pro Lys Asn Asn Asn Gln Lys   1 5 10 15 Lys Ser Ala Lys Asn Gly Ser              20 <210> 10 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> K002-2 peptide <400> 10 Tyr Thr Pro Lys Asn Asn Asn Gln Lys Lys Ser Ala Lys Asn Gly Ser   1 5 10 15 Gly gly <210> 11 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K002-3 peptide <400> 11 Glu Ala Lys Ala Asp Asp Gly Gly Ser Ser Met Glu Asn Gly Asp Asp   1 5 10 15 Asp Arg Asn Leu Lys Asp Glu              20 <210> 12 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K002-4 peptide <400> 12 Ala Ile Leu Glu Glu Gln Glu Leu Ala Glu Lys Leu Asn Gln Gln Glu   1 5 10 15 Ser Ala Gly Lys Val Thr Thr              20 <210> 13 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K002-5 peptide <400> 13 Ser Asp Arg Gln Val Gly Met Lys Ser Lys Arg Glu Glu Glu Glu Glu   1 5 10 15 Glu Glu Ala Ser Val Ala Ala              20 <210> 14 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K002-6 peptide <400> 14 Tyr Lys Val Asp Leu Asn Val Glu Ala Glu Glu Ala Glu Gln Asp Glu   1 5 10 15 Asn Asp Val Asp Trp Gln Glu              20 <210> 15 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K003-1 peptide <400> 15 Val Lys Leu Val Arg Leu Leu Val Arg Ile Phe Tyr Asp Asn Tyr Thr   1 5 10 15 Pro Glu Ser Asp Asn Gln Gln              20 <210> 16 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> K003-2 peptide <400> 16 Leu Leu Val Arg Ile Phe Tyr Asp Asn Tyr Thr Pro Glu Ser Asp Asn   1 5 10 15 Gln Gln <210> 17 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K003-3 peptide <400> 17 Asn Val Asp Gly Glu Ala Ala Lys Leu Ser Asp Asp Lys Lys Ser Asp   1 5 10 15 Glu Tyr Leu Lys Ala Tyr Tyr              20 <210> 18 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K003-4 peptide <400> 18 Thr Tyr Ser Asp Arg Gln Val Gly Met Lys Ser Lys Arg Glu Glu Glu   1 5 10 15 Glu Glu Asp Val Glu Trp Glu              20 <210> 19 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K003-5 peptide <400> 19 Asn Val Glu Ala Glu Glu Ala Glu Glu Lys Glu Asp Gly Asp Glu Asp   1 5 10 15 Asp Asp Ile Asp Trp Glu Glu              20 <210> 20 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K004-1 peptide <400> 20 Cys Gln Ser Thr Leu Ser Ser Ile Ala Ser Ser Arg Glu Arg Thr Ser   1 5 10 15 Ser Ser Arg Gln Asn Val Pro              20 <210> 21 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K004-2 peptide <400> 21 Ser Thr Leu Ser Ser Ile Ala Ser Ser Arg Glu Arg Thr Ser Ser Ser   1 5 10 15 Arg Gln Asn Val Pro Leu Pro              20 <210> 22 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K004-3 peptide <400> 22 Arg Arg Ala Ala Ala Gln Thr His Gly Ile Ser Asn Lys Pro Lys Asp   1 5 10 15 Lys Lys Lys Lys Lys Gln Glu              20 <210> 23 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K004-4 peptide <400> 23 Ala Gln Thr His Gly Ile Ser Asn Lys Pro Lys Asp Lys Lys Lys Lys   1 5 10 15 Lys Gln Glu Ile Ser Lys Arg              20 <210> 24 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K005-1 peptide <400> 24 Phe Leu Asp Leu Ala Tyr Phe Glu Gly Val Lys Thr Glu Ser Tyr Asp   1 5 10 15 Glu Asn Val Leu Ile Asp Ile              20 <210> 25 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K005-2 peptide <400> 25 Ser Val Gly Lys Val Asp Asp Ile Met Ala Gln Asn Lys Pro Asn Lys   1 5 10 15 Lys Leu Glu Ile Gln Ile Arg              20 <210> 26 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K005-3 peptide <400> 26 Ala Asp Phe Arg Leu Lys Arg Ser Thr Ser Ile Pro Lys Tyr Ser Ser   1 5 10 15 Ser Thr Phe Glu Ile Lys Phe              20 <210> 27 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K005-4 peptide <400> 27 Ser Thr Val Ala Arg Phe Pro Tyr Val Cys Arg Glu Gly Glu Thr Asp   1 5 10 15 Tyr Glu Ala Ile Tyr Val Thr              20 <210> 28 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K006-1 peptide <400> 28 Arg Thr Lys Cys Leu Leu Asn Asn Ala Glu Ile Pro Pro His His Pro   1 5 10 15 Thr Asp Pro Val Glu Met Arg              20 <210> 29 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K006-2 peptide <400> 29 Cys Leu Leu Asn Asn Ala Glu Ile Pro Pro His His Pro Thr Asp Pro   1 5 10 15 Val Glu Met Arg Arg Ile Asn              20 <210> 30 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K007-1 peptide <400> 30 Leu Glu Gln Ile Leu Glu Ala Ile Asn Gln Gly Thr Arg Arg His Arg   1 5 10 15 Arg Gln Thr Ser Lys Ile Lys              20 <210> 31 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> K007-2 peptide <400> 31 Leu Ser Tyr Thr Cys Phe Val Leu Ala Ala Leu Glu Asp Gly Asp Thr   1 5 10 15 Lys Lys Tyr Ala Ala Ser              20 <210> 32 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K007-3 peptide <400> 32 Asp Asp Ile Val Val Glu Val Ser Ser Leu Ala Lys Gln Gln Glu Glu   1 5 10 15 Pro Glu Met Leu Trp Val Met              20 <210> 33 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K007-4 peptide <400> 33 Ile Ile Ile Ile Val Ile Ala Ile Leu Leu Phe Lys Arg Lys Arg Thr   1 5 10 15 Asn Ser Pro Pro Gly Lys Asp              20 <210> 34 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K008-1 peptide <400> 34 Lys Ile Lys Gly Tyr Tyr Ile Val Ile Val Pro Leu Lys Lys Ser Arg   1 5 10 15 Gly Lys Phe Ile Lys Pro Trp              20 <210> 35 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K008-2 peptide <400> 35 Phe Ile Ile Cys Ile Val Ile Ala Ile Leu Leu Tyr Lys Arg Lys Arg   1 5 10 15 Thr Glu Ser Asp Ser Arg Lys              20 <210> 36 <211> 25 <212> PRT <213> Artificial Sequence <220> <223> K008-3 peptide <400> 36 Ile Val Ile Ala Ile Leu Leu Tyr Lys Arg Lys Arg Thr Glu Ser Asp   1 5 10 15 Ser Arg Lys Ser Ser Leu Pro Asn Ser              20 25 <210> 37 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K009-1 peptide <400> 37 Gly Gly His Glu Lys Leu Leu Leu Pro Gly Leu Gln Gly Thr Asp Lys   1 5 10 15 Gly Ser Gly Leu Ala Gly Gly              20 <210> 38 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K009-2 peptide <400> 38 Gly Ala Gly Gly Gly Gly Tyr Val Gln Ile Ile Ser Ser Pro Gly Pro   1 5 10 15 Pro Leu His His His Gly Leu              20 <210> 39 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K009-3 peptide <400> 39 Ala Arg Ala Gly Gln Leu Gln Pro Val Leu Gly Arg Pro Pro Ala Lys   1 5 10 15 Arg Arg Leu Met Glu Asn Val              20 <210> 40 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> K010-1 peptide <400> 40 Met Ile Tyr Met Val Ser Val Ser Met Met Val Leu Val Leu   1 5 10 <210> 41 <211> 17 <212> PRT <213> Artificial Sequence <220> <223> K010-2 peptide <400> 41 Met Val Ser Val Ser Met Met Val Leu Val Leu Gly Leu Val Ala Val   1 5 10 15 Ala <210> 42 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> K010-3 peptide <400> 42 Ile Asn Phe Val Cys Gly Ile Trp Val Asn Ser Lys Ser Met Trp Trp   1 5 10 15 Glu Ser Ser Cys Val              20 <210> 43 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K011-1 peptide <400> 43 Phe Asn Met Asn Ile Met Asn Leu His His Leu Ile Lys Thr Ser Val   1 5 10 15 Lys Thr Ala Phe Leu Ile Ser              20 <210> 44 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K012-1 peptide <400> 44 Leu Thr Ala Ser Tyr Ser Leu Tyr Met Phe Leu Met Thr Gln Arg Gly   1 5 10 15 Met Thr Pro Glu His Leu Asn              20 <210> 45 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K012-2 peptide <400> 45 Arg Gly Met Thr Pro Glu His Leu Asn Ala Ile Asn Pro Thr His Thr   1 5 10 15 Arg Glu His Thr Leu Met Thr              20 <210> 46 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> K013-1 peptide <400> 46 Ser Thr Ile Leu Ala Ile Leu Ser Phe Trp Leu Pro Gln Met Thr Pro   1 5 10 15 Asp met <210> 47 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K013-2 peptide <400> 47 Ile Ala Leu Leu Leu Pro Phe Pro Trp Ala Ala Gln Leu Asn Thr Pro   1 5 10 15 Ser Ile Val Ile Leu Trp Ala              20 <210> 48 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K014-1 peptide <400> 48 Asp Pro Phe Thr Leu Ile Ser Trp Pro Ile Gln Ser Asn Gly Phe Asn   1 5 10 15 Asn Arg Leu Ile Thr Leu Gln              20 <210> 49 <211> 29 <212> PRT <213> Artificial Sequence <220> <223> K014-2 peptide <400> 49 Leu His Asn Phe Thr Thr Ile Phe Tyr Gln Leu Thr Ser Pro Gly His   1 5 10 15 Lys Trp Ala Leu Leu Leu Thr Ser Leu Met Leu Leu Leu              20 25 <210> 50 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K015-1 peptide <400> 50 Met Met Met Phe Ile Ile Trp Glu Ala Phe Ala Ala Lys Arg Glu Val   1 5 10 15 Thr Thr Tyr Glu Leu Thr Ser              20 <210> 51 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K015-2 peptide <400> 51 Ala Phe Ala Ala Lys Arg Glu Val Thr Thr Tyr Glu Leu Thr Ser Thr   1 5 10 15 Met Leu Glu Trp Leu Gln Gly              20 <210> 52 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K015-3 peptide <400> 52 Tyr Glu Leu Thr Ser Thr Met Leu Glu Trp Leu Gln Gly Cys Pro Thr   1 5 10 15 Pro Tyr His Thr Leu Lys Thr              20 <210> 53 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> K016-1 peptide <400> 53 Met Asn Pro Ile Thr Phe Ser Val Val Leu Thr Ser Leu Ala Ser Glu   1 5 10 15 Gln Phe Leu Ala Val Ser              20 <210> 54 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> K016-2 peptide <400> 54 Val Leu Thr Ser Leu Ala Ser Glu Gln Phe Leu Ala Val Ser Ser Ser   1 5 10 15 His Trp Leu Leu Ala Trp              20 <210> 55 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K016-3 peptide <400> 55 Gly Phe Val Pro Lys Trp Phe Ile Ile Gln Glu Leu Thr Ser Gln Asn   1 5 10 15 Thr Thr Ile Leu Ala Thr Thr              20 <210> 56 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K016-4 peptide <400> 56 Phe Tyr Leu Arg Leu Thr Tyr Ile Val Thr Leu Thr Ser Ser Pro Asn   1 5 10 15 Thr Ser Asn Ala Ser Leu Thr              20 <210> 57 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K016-5 peptide <400> 57 Ser Pro Asn Thr Ser Asn Ala Ser Leu Thr Trp Arg His His Ser Lys   1 5 10 15 Gln Pro Thr Leu Leu Leu Ser              20 <210> 58 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K017-1 peptide <400> 58 Ile Val Leu Val Leu Gly Leu Val Ala Val Ala Ser Asn Pro Ser Pro   1 5 10 15 Phe Tyr Ala Ala Leu Gly Leu              20 <210> 59 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-1 peptide <400> 59 His Asn Lys Ile Pro Pro Arg Trp Leu Asn Cys Pro Arg Arg Gly Gln   1 5 10 15 Pro Val Ala Ala Lys Phe Leu              20 <210> 60 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-2 peptide <400> 60 Leu Pro Leu Lys Thr Met Leu Gly Pro Lys Tyr Asp Asp Gln Val Pro   1 5 10 15 Glu Glu Asn Arg Phe His Pro              20 <210> 61 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-3 peptide <400> 61 Lys Pro Pro Glu Leu Pro Asp Trp Cys Phe Glu Glu Glu Asp Val Asp   1 5 10 15 Asp Glu Gly Asn Asn Val Phe              20 <210> 62 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-4 peptide <400> 62 Glu Ala Glu Ala Gly Ser Ser Gly Ala Thr Tyr Asn Arg Arg Lys Lys   1 5 10 15 Glu Arg Leu Lys Leu Gly Ala              20 <210> 63 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-5 peptide <400> 63 Gly Leu Leu Thr Arg Asn Val Gly Leu Leu Tyr Val Gly Lys Tyr Asp   1 5 10 15 Cys Pro Phe Ser Glu Ile Lys              20 <210> 64 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K018-6 peptide <400> 64 Val Gly Leu Leu Tyr Val Gly Lys Tyr Asp Cys Pro Phe Ser Glu Ile   1 5 10 15 Lys Asp Leu Tyr Leu Met Gly              20 <210> 65 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K019-1 peptide <400> 65 His Asn Lys Ile Pro Pro Arg Trp Leu Asn Cys Pro Arg Arg Gly Gln   1 5 10 15 Pro Val Ala Ala Lys Phe Leu              20 <210> 66 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K019-2 peptide <400> 66 Leu Pro Leu Lys Thr Met Leu Gly Pro Lys Tyr Asp Asp Gln Val Pro   1 5 10 15 Glu Glu Asn Arg Phe His Pro              20 <210> 67 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K019-3 peptide <400> 67 Pro Lys Pro Pro Glu Leu Pro Asp Trp Cys Phe Glu Glu Glu Asp Val   1 5 10 15 Asp Asp Glu Gly Asn Asn Val              20 <210> 68 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K020-1 peptide <400> 68 His Asn Lys Ile Pro Pro Arg Trp Leu Asn Cys Pro Arg Arg Gly Gln   1 5 10 15 Pro Val Ala Ala Lys Phe Leu              20 <210> 69 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K020-2 peptide <400> 69 Leu Pro Leu Lys Thr Met Leu Gly Pro Lys Tyr Asp Asp Gln Val Pro   1 5 10 15 Glu Glu Asn Arg Phe His Pro              20 <210> 70 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K020-3 peptide <400> 70 Lys Pro Pro Glu Leu Pro Asp Trp Cys Phe Glu Glu Glu Asp Val Asp   1 5 10 15 Asp Glu Gly Asn Asn Val Phe              20 <210> 71 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K020-4 peptide <400> 71 Glu Ala Glu Ala Gly Ser Ser Gly Ala Thr Tyr Asn Arg Arg Lys Lys   1 5 10 15 Glu Arg Leu Lys Leu Gly Ala              20 <210> 72 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K020-5 peptide <400> 72 Asp Phe Ile Asp Arg Cys Thr Ala Ala Asn His Arg Gln Asn Arg Lys   1 5 10 15 Asn Pro Leu Asp Pro Asp Ala              20 <210> 73 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> K021-1 peptide <400> 73 Leu Leu Pro Leu Leu Ser Gly Val His Cys Asp Val Gln Leu Ala Gln   1 5 10 15 Ser Glu Ser <210> 74 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K022-1 peptide <400> 74 Ala Lys Leu Lys Ala Asp Tyr Asn Leu Pro Met His Lys Met Thr Asn   1 5 10 15 Thr Asp Leu Thr Arg Ile Leu              20 <210> 75 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K022-2 peptide <400> 75 Gln Arg Ala Leu Arg Ala Pro Asn Lys Lys Val Lys Arg Arg Glu Leu   1 5 10 15 Lys Lys Asn Pro Leu Lys Asn              20 <210> 76 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-1 peptide <400> 76 Ser Leu Arg Pro Pro Val Pro Gly Gln Glu Thr Asn Asn Phe Lys Pro   1 5 10 15 Glu Lys Phe Thr Met Asp Ser              20 <210> 77 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-2 peptide <400> 77 Lys Phe Thr Met Asp Ser Gln His Leu Lys His Lys Glu Asp Phe Asn   1 5 10 15 Arg Glu Ala Glu Gly Cys Val              20 <210> 78 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-3 peptide <400> 78 Thr Asp Gly Ser Val Asp Gly Ile Pro Pro Tyr Arg Ser Lys Lys Gln   1 5 10 15 Arg Glu Ile His Arg Ser Val              20 <210> 79 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-4 peptide <400> 79 Ala Lys Leu Thr Ile Ala Leu Glu Lys Val Lys Lys Gln Arg Asp Ala   1 5 10 15 Glu Glu Lys Leu Glu Glu Lys              20 <210> 80 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-5 peptide <400> 80 Val Gln Arg Lys Gly Lys Phe Gln Pro Ala Phe Ser Lys Gly Gln Thr   1 5 10 15 Ser Thr Ser Cys His Leu Arg              20 <210> 81 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-6 peptide <400> 81 Thr Ser Ala Ala Val Leu Thr Thr Pro Leu Ser Pro Glu Gln Glu Ala   1 5 10 15 Glu Arg Asn His Ser Val Leu              20 <210> 82 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-7 peptide <400> 82 Lys Ser Arg Cys Val Gln Ser Thr Thr Leu Lys Glu Lys Gly Lys Thr   1 5 10 15 Ala Glu Ser Val Pro Ser Ser              20 <210> 83 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K023-8 peptide <400> 83 Val Pro Ser Ser Gly Phe Ser Thr Leu Lys Leu Ser Glu Glu Gln Lys   1 5 10 15 Thr Ala Lys Lys Pro Ser Ser              20 <210> 84 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K024-1 peptide <400> 84 Glu Pro Val Phe Glu Ser Leu Glu Pro Tyr Lys Gly Pro Gly Arg Glu   1 5 10 15 Asp Asn Ala Met Gln Ser Pro              20 <210> 85 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K024-2 peptide <400> 85 Ser Val Arg Ala Tyr Leu Gly Tyr Gln Thr Val Pro Ser Gly Ser Ser   1 5 10 15 Gly Asn Leu Ser Ser Ala Ser              20 <210> 86 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K024-3 peptide <400> 86 Ser Gly Ser Ser Gly Asn Leu Ser Ser Ala Ser Ser Ser Ser Ser Pro   1 5 10 15 Pro Gly Thr Pro Asn Pro Leu              20 <210> 87 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K024-4 peptide <400> 87 Ser Ser Ser Pro Pro Gly Thr Pro Asn Pro Leu Asp Ser Lys Ser Glu   1 5 10 15 Gly Pro Ser Gly Ala Ser Gly              20 <210> 88 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K024-5 peptide <400> 88 Glu Gly Pro Ser Gly Ala Ser Gly Thr Gly Tyr Arg Lys Ser Gly Ser   1 5 10 15 Gly Lys Ala Lys Lys Ser Leu              20 <210> 89 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> K024-6 peptide <400> 89 Ser Gly Ser Gly Lys Ala Lys Lys Ser Leu Asp Lys Gln Ser Asn Asp   1 5 10 15 Tyr Lys Leu Arg              20 <210> 90 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K025-1 peptide <400> 90 Ser His Leu Leu Gln Arg Cys Ser Ala Gln Leu Cys Thr Arg Gly Ser   1 5 10 15 Ser Leu Ile Leu Ser Leu Leu              20 <210> 91 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K025-2 peptide <400> 91 Leu Leu Leu Ser Val Cys Leu Ser Val Glu Gly Gln Tyr Asn Gly Glu   1 5 10 15 Lys Gly Ile Ser Ile Pro Asp              20 <210> 92 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K025-3 peptide <400> 92 Pro Ala Val Pro Glu Thr Trp Thr Ser Asn Ser Arg Thr Tyr Tyr Arg   1 5 10 15 Asp Lys Phe Met Cys Pro Arg              20 <210> 93 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K025-4 peptide <400> 93 Lys Val Pro Ala Tyr Val Asn Tyr His Phe Leu Gly Glu Lys Asp Cys   1 5 10 15 Gly Ala Pro Cys Glu Val Gly              20 <210> 94 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K026-1 peptide <400> 94 Asp Gly Asp Ser Lys Ala Glu Lys Ala Lys Ala Lys Asp Glu Pro Gln   1 5 10 15 Arg Arg Ser Ala Arg Leu Ser              20 <210> 95 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> K026-2 peptide <400> 95 Asp Lys Ala Pro Lys Gly Lys Lys Gly Lys Ala Asp Ser Gly Lys Asp   1 5 10 15 Ser Ser Asn Ala Ala Glu Asn              20 <210> 96 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 96 Arg Lys Gln Val Asn Ala Asp Val Ala Asp Ala Lys Asp Glu Pro Lys   1 5 10 15 Arg Arg Ser Ala Arg Leu Ser              20 <210> 97 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 97 Leu Ser Ser Lys Pro Thr Pro Ala Lys Thr Glu Pro Lys Pro Lys Lys   1 5 10 15 Glu Lys Ala Pro Val Lys Glu              20 <210> 98 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 98 Pro Lys Lys Glu Lys Ala Pro Val Lys Glu Lys Pro Glu Glu Lys Glu   1 5 10 15 Lys Lys Val Pro Ala Lys Gly              20 <210> 99 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 99 Glu Glu Ala Asn Lys Glu Glu Ala Asn Glu Asp Gln Pro Ser Glu Asn   1 5 10 15 Gly Glu Thr Lys Ser Asp Glu              20 <210> 100 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 100 Lys Arg Lys Arg Glu Trp Ser Asp Glu Ser Glu Glu Glu Pro Glu Lys   1 5 10 15 Glu Leu Ala Pro Glu              20 <210> 101 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 101 Phe Cys Trp Lys Arg Lys Met Glu Trp Trp Asp Lys Ser Glu Glu Ser   1 5 10 15 Glu Glu Glu Pro Arg Lys Val              20 <210> 102 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 102 Phe Leu Ala Leu Tyr Leu Ala Asn Asp Met Glu Glu Asp Asp Glu Asp   1 5 10 15 Ser Lys Gln Asn Ile Phe His              20 <210> 103 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 103 Ser Asp Gly Thr Thr Ser Arg Lys Leu Arg Tyr Ser His His Asp Lys   1 5 10 15 Lys Asn Gly Arg Ser Ser Asn              20 <210> 104 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 104 Arg Lys Leu Arg Tyr Ser His His Asp Lys Lys Asn Gly Arg Ser Ser   1 5 10 15 Asn Gly Ala Leu Arg Gly Val              20 <210> 105 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 105 Leu Glu Ile Lys Ala Cys Val Leu Met Asn Met Lys Glu Lys Gly Thr   1 5 10 15 Glu Thr Val Lys Ala Glu Lys              20 <210> 106 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 106 Met Gly Arg Lys Lys Lys Leu Pro Thr Gly Val Ser Ser Gly   1 5 10 <210> 107 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 107 Asn Leu Glu Met Val Glu Leu Leu Val Val Met Gly Val Glu Thr Lys   1 5 10 15 Asp Ala Leu Leu His Ala Ile              20 <210> 108 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 108 Glu Leu Leu Leu Glu His Glu Glu Leu Ile Tyr Lys Glu Gly Glu Pro   1 5 10 15 Tyr Ser Trp Gln Lys Val Asp              20 <210> 109 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 109 Leu Glu His Glu Glu Leu Ile Tyr Lys Glu Gly Glu Pro Tyr Ser Trp   1 5 10 15 Gln Lys Val Asp Ile Asn Thr              20 <210> 110 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 110 Leu Ser Trp Glu Leu Arg Asn Leu Ala Leu Thr Glu Gln Glu Cys Lys   1 5 10 15 Ser Glu Tyr Met Asp Leu Arg              20 <210> 111 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 111 Gln Cys Gln Lys Phe Ala Val Asp Leu Leu Asp Gln Thr Arg Thr Ser   1 5 10 15 Asn Glu Leu Ala Ile Ile Leu              20 <210> 112 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 112 Arg Met Ser Leu Thr Arg Leu Val Gln Ala Ile Ser Tyr Lys Gln Lys   1 5 10 15 Lys Phe Val Ala His Ser Asn              20 <210> 113 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 113 Ser Ser Lys Thr Ile Asp Arg Gln Arg Ser Lys Lys Arg Lys Glu Gln   1 5 10 15 Glu Gln Phe Ser Glu Tyr Asp              20 <210> 114 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 114 Glu Asn Ser Gly Met Asp Val Ser Ser Ala Asn Lys Lys Glu Arg Gln   1 5 10 15 Pro Arg Pro Arg Arg Ile Lys              20 <210> 115 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 115 Ser Gly Arg Glu Ser Pro Ile Arg Ile Glu Phe Glu Asp Glu Lys Thr   1 5 10 15 Ser Thr Leu Leu Glu Leu Leu              20 <210> 116 <211> 24 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 116 Arg Ser Leu Thr Ala Pro Glu Leu Lys Ile Ser Arg Lys Ser Ser Pro   1 5 10 15 Ala Pro Thr Pro Thr Pro Thr Pro              20 <210> 117 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 117 Ser Asp Ile Ser Ser Ile Ala Ser Thr Ser Pro Gln Arg Pro Lys His   1 5 10 15 Arg asn <210> 118 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 118 Pro Gln Leu Gln Arg Ile Gln Pro Tyr Leu Val Pro Gln Arg Asn Arg   1 5 10 15 Ser Gln Ala Gly Gly Leu Leu              20 <210> 119 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 119 Gln Pro Ser Leu Leu Ser Ser Ala Leu Met Pro Lys Arg Lys Arg Gly   1 5 10 15 Arg Pro Arg Lys Leu Ser Gly              20 <210> 120 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 120 Asn Gly Lys Met Cys Asn Glu Ser Tyr Ser Gly Asn Asp Asp Gly Ser   1 5 10 15 Asp Asp Asn Gln Pro Asn Ala              20 <210> 121 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 121 Asp Leu Asn Glu Ser Arg Asp Ser Leu Pro Ser Lys Arg Ser Lys Asn   1 5 10 15 Ser Lys Asp His Arg Val Val              20 <210> 122 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 122 Gln Ser Leu Pro Ala Thr Ser Asn Ala Leu Ser Thr Ser Ser Ser Ser   1 5 10 15 Ser Thr Ser Ser Ser Ser Gly              20 <210> 123 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 123 Pro Gln Leu Gln Arg Ile Gln Pro Tyr Leu Val Pro Gln Arg Asn Arg   1 5 10 15 Ser Gln Ala Gly Gly Leu Leu              20 <210> 124 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 124 Pro Ser Leu Leu Ser Ser Ala Leu Met Pro Lys Arg Lys Arg Gly Arg   1 5 10 15 Pro Arg Lys Leu Ser Gly Ser              20 <210> 125 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 125 Gly Lys Met Cys Asn Glu Ser Tyr Ser Gly Asn Asp Asp Gly Ser Asp   1 5 10 15 Asp Asn Gln Pro Asn Ala Gly              20 <210> 126 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 126 Asp Leu Asn Glu Ser Arg Asp Ser Leu Pro Ser Lys Arg Ser Lys Asn   1 5 10 15 Ser Lys Asp His Arg Val Val              20 <210> 127 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 127 Gln Ser Leu Pro Ala Thr Ser Asn Ala Leu Ser Thr Ser Ser Ser Ser   1 5 10 15 Ser Thr Ser Ser Ser Ser Gly              20 <210> 128 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 128 Pro Gln Leu Gln Arg Ile Gln Pro Tyr Leu Val Pro Gln Arg Asn Arg   1 5 10 15 Ser Gln Ala Gly Gly Leu Leu              20 <210> 129 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 129 Gln Pro Ser Leu Leu Ser Ser Ala Leu Met Pro Lys Arg Lys Arg Gly   1 5 10 15 Arg Pro Arg Lys Leu Ser Gly              20 <210> 130 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 130 Pro Lys Arg Lys Arg Gly Arg Pro Arg Lys Leu Ser Gly Ser Ser Asn   1 5 10 15 Gly Thr Gly Asn Asp Tyr Asp              20 <210> 131 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 131 Lys Arg Gly Arg Pro Arg Lys Leu Ser Gly Ser Ser Asn Gly Thr Gly   1 5 10 15 Asn Asp Tyr Asp Asp Phe Asp              20 <210> 132 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 132 Arg Pro Arg Lys Leu Ser Gly Ser Ser Asn Gly Thr Gly Asn Asp Tyr   1 5 10 15 Asp Asp Phe Asp Arg Glu Asn              20 <210> 133 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <133> 133 Asn Asn Ser Met Leu Asn Thr Thr Asp Tyr Tyr Gln Gln Gln Gln Gln   1 5 10 15 Glu Ser Pro Ser Ser Ala Gly              20 <210> 134 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 134 Met Asp Asp Asp Leu Glu Leu Leu Ser Leu Asn Asp Gln Gln Asp Lys   1 5 10 15 Ser Asp Glu Pro Asp His Glu              20 <210> 135 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 135 Met Ala Ile Met Ile Val Ile Leu Cys Arg Met Gln Thr Pro His Ser   1 5 10 15 Lys Gln Thr Leu Gln Thr Pro              20 <210> 136 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 136 Ala Lys Phe Pro Leu Ile Arg Gln Phe Ser Leu Glu Ser Ser Ser Ser   1 5 10 15 Gly lys <210> 137 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 137 Ile Asp Asn Leu Leu Gln Glu Met Leu Leu Gly Gly Ser Ala Asn Glu   1 5 10 15 Ala Ser His Thr His His His              20 <210> 138 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 138 Ser Gln Leu His Asn Gly Gln Met Ser Thr Pro Glu Thr Pro Gln Pro   1 5 10 15 Ser Pro Pro Ala Gly Ser Gly              20 <139> <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 139 Gly Thr Ile Ala Ser Ile Asn Lys Gln Pro Thr Ser Ile Pro Gln Ser   1 5 10 15 Thr Ile Thr Lys Gln Glu Ala              20 <210> 140 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 140 Gln Gly Met Gly Ile Cys Ala Gln Cys Pro Ala Asn Ser Arg Ser Thr   1 5 10 15 Ser Glu Ala Ser Pro Ile Cys              20 <210> 141 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 141 Ile Ile Val Ile Val Cys Phe Ser Arg Lys Gln Arg Asn Asp Ser Glu   1 5 10 15 Ser Glu Tyr Thr              20 <210> 142 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 142 His Lys Met Thr Asn Thr Asp Leu Thr Arg Ile Leu Lys Ser Gln Glu   1 5 10 15 Ile Gln Arg Ala Leu Arg Ala              20 <210> 143 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 143 Ile Leu Gln Gln Leu Glu Asn Ile Lys Ala Lys Glu Lys Lys Pro Asp   1 5 10 15 Asp Gly Lys Pro Lys Ala Lys              20 <210> 144 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 144 Lys Tyr Ser Gln Tyr Ile Pro Ala Asn Arg Tyr Pro Lys Asn Glu Asp   1 5 10 15 Thr Tyr Pro Glu Lys              20 <210> 145 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 145 Asn Arg Tyr Pro Lys Asn Glu Asp Thr Tyr Pro Glu Lys Thr Ser Glu   1 5 10 15 Thr Ala Thr Ala Pro Phe Gln              20 <210> 146 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 146 Asn Phe Asn Thr Glu Gln Gln Leu Ala Ile Ser Gly Asn Thr Gln Ser   1 5 10 15 Pro Leu Gly His Tyr Ser Leu              20 <210> 147 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 147 His Ser Thr Val Glu Lys Ala Ser Thr Arg Ala Ser Ser Ser Ala Ser   1 5 10 15 Lys Lys Gln Tyr Val              20 <210> 148 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 148 Val Pro Gly Gln Pro Thr Pro Asp Gly Cys Gln Gln Gln Glu Gly Gly   1 5 10 15 Gly Glu Asn Thr Asn Ser Ile              20 <210> 149 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 149 Gly Glu Asn Thr Asn Ser Ile Ser Ser Asn Gly Glu Asp Ser Asp Glu   1 5 10 15 Ala Gln Met Arg Leu Gln Leu              20 <210> 150 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 150 Glu Glu Glu Leu Arg Asn Gln Arg Arg Gln Ala Ser Asn Thr Pro Ser   1 5 10 15 His ile <210> 151 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 151 Ile Ser Ser Ser Phe Ser Thr Ser Val Tyr Gln Pro Ile Pro Gln Pro   1 5 10 15 Thr Thr Pro Val Ser Phe Thr              20 <210> 152 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 152 Tyr Phe Cys Met Leu Pro Phe Ser Pro Ser Val Asn Gly Arg Ser Tyr   1 5 10 15 Asp Thr Tyr Thr Pro Leu His              20 <210> 153 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 153 Lys Val His Cys Leu Ser Glu Ile Ile Phe Arg Thr Lys Gln Gln Gln   1 5 10 15 Arg Glu Leu Arg Ala His Val              20 <210> 154 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 154 Ser Glu Ser Ile Thr Val Glu Trp Ile Glu Asn Gly Asp Thr Lys Gly   1 5 10 15 Lys Glu Ile Asp Leu Glu Ser              20 <210> 155 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 155 Asn Arg Arg Thr Val Ala Pro Val Lys Asn Glu Thr Pro Ala Lys Asp   1 5 10 15 Asn Arg Val Ala Ala Val Gly              20 <210> 156 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 156 Gln Pro Ile Glu Gln Ser Ala Ser Arg Gln Gln Asn Gly Ser Val Ser   1 5 10 15 Asp Ile Ser <210> 157 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 157 Gln Gln Asn Gly Ser Val Ser Asp Ile Ser Pro Asp Gln Pro Gly Lys   1 5 10 15 Lys Asp Phe Gly Leu Ala Ser              20 <210> 158 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 158 Ser Asn Cys Val Lys Glu Val Glu Lys Leu Gln Glu Lys Arg Glu Arg   1 5 10 15 Arg Arg Leu Gln Gln Gln Glu              20 <210> 159 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 159 Glu Leu Asp Pro Ser Arg Cys Arg Arg Pro Pro Pro His Asp Thr Ser   1 5 10 15 Cys Pro Gln <210> 160 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 160 Ala Pro Gln Arg Asp Asp Leu Lys Leu Leu Cys Glu Gln Asn Glu Glu   1 5 10 15 Glu Val Ser Pro Gln Leu Phe              20 <210> 161 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 161 Leu Gln Glu Glu Glu His Ala Ser Lys Gln Ile Asn Pro Lys Arg Pro   1 5 10 15 Arg Ala Leu <210> 162 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 162 Leu Ile Ile Gln Ser Leu Phe Ala Phe Leu Glu His Arg Lys Met Lys   1 5 10 15 Lys Ser Leu Glu Thr Pro Ile              20 <210> 163 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 163 Phe Ala Ile Ala Thr Ile Asn Lys Ala Gly Trp Gly Thr Ser Gly Arg   1 5 10 15 Lys Thr Ile Val Val Asn Phe              20 <210> 164 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 164 Thr Ile Leu Leu Gly Gly Val Ile Phe Thr Ile Tyr Lys Glu Ser Lys   1 5 10 15 Lys Pro Phe Ser Glu Ser Lys              20 <210> 165 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 165 Phe Thr Ile Tyr Lys Glu Ser Lys Lys Pro Phe Ser Glu Ser Lys Gln   1 5 10 15 Thr Val Leu Ile Val Gly Thr              20 <210> 166 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 166 Tyr Val Val Leu Ile Asn Lys Cys Gly Arg Arg Lys Lys Gly Gln Gln   1 5 10 15 Tyr Asp Met Val Leu Asp Val              20 <210> 167 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 167 His Leu Leu Ile Gln Ser Leu Phe Ala Phe Leu Glu His Arg Arg Met   1 5 10 15 Arg Arg Ala Gly Arg Pro Leu              20 <210> 168 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 168 Arg Arg Ala Gly Arg Pro Leu Lys Leu His Cys Ser Gln Arg Ser Arg   1 5 10 15 Ser val ala leu              20 <210> 169 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 169 Phe Ala Ile Ala Thr Ile Asn Lys Ser Gly Trp Gly Thr Ser Gly Arg   1 5 10 15 Lys Thr Ile Val Val Asn Phe              20 <210> 170 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 170 Leu Tyr Leu Ala Ile Ile Ala Arg Arg Cys Gly Lys Lys Pro Glu Gln   1 5 10 15 Tyr Ser Leu Ala Phe Ala Glu              20 <210> 171 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 171 Phe Ile Ala Ala Leu Asp Asp Ile Thr Val Asp Leu Pro Pro Val Glu   1 5 10 15 Asp Tyr <210> 172 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 172 Leu Gly Asn Asn Thr Ala Cys Gly Thr Trp Val Gly Gln Lys Pro Asp   1 5 10 15 Gly Ser Leu Val Val Asn Ala              20 <210> 173 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 173 Val Val Asn Ala Ala Tyr Asp Gly Cys Tyr Val Arg Glu Glu Asn Gly   1 5 10 15 Asp His Val Met Thr Val Ser              20 <210> 174 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 174 Ser Leu Glu Glu Val Ile Asn Gly His Val Asp Tyr His Lys Gln Glu   1 5 10 15 Leu Lys Cys Pro Ile Met Glu              20 <175> 175 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 175 Ser Asp Gly Pro Val Asp Phe Ile Asp Gly Glu Glu Lys Arg Thr Glu   1 5 10 15 Thr Glu Gly Val Val Gly Phe              20 <210> 176 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 176 Thr Val Thr Phe Ile Gly Leu Trp Ile His Tyr Arg Asn Arg Ser Pro   1 5 10 15 Lys Thr His Asp Val Asn Ala              20 <210> 177 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 177 Val Phe Pro Gln Ile Leu Arg Ala Leu Glu Glu Asp Pro Glu Asp Asn   1 5 10 15 His Arg Leu Phe Ala Gln Asn              20 <210> 178 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 178 Phe Ala Gln Asn Pro Val Asp Val Thr Ser Leu Arg Pro Ser Asp Leu   1 5 10 15 Glu Glu Phe Val Lys Gly              20 <210> 179 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 179 Leu Leu Pro Asn Val Asp Ser Ile Ser Arg Ser Val Gln Asp Gln Glu   1 5 10 15 Asp Asp Val Pro Ile Ile Asp              20 <210> 180 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 180 His Ile Ser Ser Val Glu Thr Thr Lys Val Ala Ala Arg Gly Arg Lys   1 5 10 15 Lys Gln Ile Ile Gln Ser Trp              20 <210> 181 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 181 Gly Arg Lys Lys Gln Ile Ile Gln Ser Trp Asn Trp Glu Pro Gln Arg   1 5 10 15 Gly Arg Met Leu Asn Leu Ile              20 <210> 182 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 182 Glu Phe Glu Glu Lys Ile Asn Lys Phe His Met Glu Lys Lys Glu Gln   1 5 10 15 Glu Glu Thr Ala Arg Asn Ala              20 <210> 183 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 183 Pro Ser Met Glu Glu Ser Gly Asp Asn Leu Val Glu Thr Glu Ser Glu   1 5 10 15 Glu Glu Pro Ser Asp Ser Glu              20 <210> 184 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 184 Leu Val Glu Thr Glu Ser Glu Glu Glu Pro Ser Asp Ser Glu Glu Glu   1 5 10 15 Pro Asp Ser Ala Gln Cys Gly              20 <210> 185 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 185 Leu Asn Gln Lys Thr Ser Gly Asp Asn Leu Ile Glu Thr Glu Pro Glu   1 5 10 15 Glu Glu Gln Ser Asp Ser Glu              20 <210> 186 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 186 Thr Ser Gly Asp Asn Leu Ile Glu Thr Glu Pro Glu Glu Glu Gln Ser   1 5 10 15 Asp Ser Glu Pro Asp Ser Ala              20 <210> 187 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 187 Leu Asn Arg Lys Thr Ser Gly Asp Asn Leu Ile Glu Thr Glu Ser Glu   1 5 10 15 Glu Glu Gln Ser Asp Ser Glu              20 <210> 188 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 188 Glu Glu Gln Ser Asp Ser Glu Glu Glu Pro Ser Asp Ser Glu Glu Glu   1 5 10 15 Pro Asp Ser Ala Gln Cys Gly              20 <210> 189 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 189 Gln Cys Gly Thr Thr Asn Pro Arg Ser Leu Asn Gln Lys Thr Ser Gly   1 5 10 15 Gly Glu Glu Gly Glu Ser Glu              20 <210> 190 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 190 Ser Gly Gly Glu Glu Gly Glu Ser Glu Ser Lys Ser Thr Glu Ser Ser   1 5 10 15 Ser Ser Ile Arg Arg Asn Leu              20 <210> 191 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 191 Pro Phe Asn Gly Ala Gly Asn Arg Met Pro Pro Gln Glu Tyr Thr Asn   1 5 10 15 Pro Pro Ser Ser Asn Gln Asp              20 <210> 192 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 192 Met Pro Pro Gln Glu Tyr Thr Asn Pro Pro Ser Ser Asn Gln Asp Ile   1 5 10 15 Asp Leu Pro Ala Tyr Asn Lys              20 <210> 193 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 193 Gly Ile Ile Ser Asn Met Asp Ala Val Lys Ser Asn His Gln Thr Ser   1 5 10 15 Ser Lys Gln Gln Tyr Val              20 <210> 194 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 194 Thr Gln Ser Met Asp Glu Ile Gly Leu Ser Pro Arg Lys Asp Ser Tyr   1 5 10 15 Gln Ile Phe Pro Asp Pro Ser              20 <210> 195 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 195 Val Asp Glu Asp Lys Glu Gly Thr Cys Asp Gln Thr Lys Lys Glu Asn   1 5 10 15 Glu gln <210> 196 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 196 Ile Asn Pro His Leu Ser Ile Gln Val Ser Ser Lys Asp Asn Glu Ser   1 5 10 15 Trp Asp Glu Gly Ile Glu Ser              20 <210> 197 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 197 His Asn Gly Thr Ile Leu Gly Glu Ala Glu Lys Glu Asp Asp Glu Ser   1 5 10 15 Gly Asn Ile Asp His Gln Leu              20 <210> 198 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 198 Arg Pro Glu Ile Gly Leu Met Gln Pro Ser Ala Pro Pro Asp Tyr Glu   1 5 10 15 His Glu Leu Ile Asn Pro His              20 <210> 199 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 199 Pro Gln Arg Arg Val Gln Ala Gln Leu Ser Pro His Tyr Gln Arg Arg   1 5 10 15 Arg Leu Pro Pro Pro Leu Glu              20 <210> 200 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 200 Pro Leu Glu Asp Gln Ile Leu Leu Glu Trp Glu Glu Arg Lys Arg Lys   1 5 10 15 Gln Pro Trp Val Phe Asn Gly              20 <210> 201 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 201 Phe Arg Leu His Ser Val Lys Ala Asp Leu Asn Gln Arg Glu Lys Ile   1 5 10 15 Asn Arg Glu Asn Thr Leu Gly              20 <210> 202 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 202 Gly Glu Ile Gly Asp His Ile Arg Gln Glu Ala Asp Met Asp Glu Gln   1 5 10 15 Gly Gly Leu Thr Leu Tyr Leu              20 <210> 203 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 203 Asn Trp Ser Gly Asn Ala Lys Ala Leu Gln Glu Arg Gly Gly Lys Glu   1 5 10 15 His Gly Asp Pro Glu Ala Tyr              20 <210> 204 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 204 Gln Cys Ser Asp Gly Arg Phe Val Leu Leu Arg Arg Ser Asn Arg Val   1 5 10 15 Gly Glu Ala Pro Gly Gln Leu              20 <210> 205 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 205 Ser Ala Glu Phe Tyr Val Arg Cys Ser Phe Ser Ser Glu Glu Val Arg   1 5 10 15 Glu Arg Tyr Leu Gln Gly Gly              20 <206> 206 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 206 Val Arg Glu Arg Tyr Leu Gln Gly Gly Pro Glu Ala Ser Glu Ser Thr   1 5 10 15 Asp Ile Met Phe Ile Asp Arg              20 <210> 207 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 207 Met Val Arg Asn Thr His Leu Asn Leu Gln Pro Gln Glu Arg Gln Ala   1 5 10 15 Phe Tyr Arg Leu Leu Glu              20 <210> 208 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 208 Val Ala Leu Tyr Leu Ala Asn Asp Met Glu Glu Asp Glu Glu Asp Tyr   1 5 10 15 Lys Tyr Glu Ile Phe Pro Trp              20 <210> 209 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 209 Val Ser Arg Arg Cys Cys Asp Glu Val Met Ser Lys Asp Pro Thr His   1 5 10 15 Trp Ala Trp Leu Arg Asp Arg              20 <210> 210 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 210 His His Ser Gly Ala Met Arg Gly Tyr Leu Arg Asn Glu Asp Asp Phe   1 5 10 15 Ser Pro Arg Gly Pro Gly Leu              20 <210> 211 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 211 Gly Val Cys Asp Ser Gly Gly Val Ser His Asn Asn Ser Ser Ser Pro   1 5 10 15 Glu Gln Glu Ile Phe His Tyr              20 <210> 212 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 212 Ser Ser Pro Glu Gln Glu Ile Phe His Tyr Thr Asn Arg Glu Trp Ser   1 5 10 15 Gln Glu Leu Leu Met Leu Ser              20 <210> 213 <211> 17 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 213 Met Gln Cys Asp Ile Gln Thr Ser Arg Arg Glu Cys Pro Ile Glu Pro   1 5 10 15 Thr <210> 214 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 214 Leu Pro Ala Ser Asn Ser Ala Val Leu Asn Pro Asp Gly Ser Asn Gln   1 5 10 15 Ser Gly Phe Ala Gln Gly Gly              20 <210> 215 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 215 Pro Tyr Asn Ser Ala Gly Asn Pro Tyr Pro Pro Ala Gly Gln Ser Ser   1 5 10 15 Pro Ala Gly Asn Gln Ser Ile              20 <210> 216 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 216 Pro Ala Gly Asn Gln Ser Ile Val Phe Gln Asn Ser Asn Gln Pro Gly   1 5 10 15 Ser Asn Thr Pro Gln Tyr Thr              20 <210> 217 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 217 Gln Asn Ser Asn Gln Pro Gly Ser Asn Thr Pro Gln Tyr Thr Ser Ser   1 5 10 15 Pro Ala Pro Ser Gly Ser Ser              20 <210> 218 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 218 Pro Gly Pro Val Gly Ala Gln Asn Ile Pro Gly Asn Tyr Pro Gln Ser   1 5 10 15 Ala Thr Ala Gly Asn Phe Asn              20 <210> 219 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 219 Ser Pro Ser Ser Gly Leu Gly Gln Phe Ser Arg Pro Ala Ser Ser Gly   1 5 10 15 Thr Pro Phe Asn Ser Gly Gln              20 <210> 220 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 220 His Gly Trp Asn Ala Ile Ser Asp Gly Lys Ser His Lys Arg Lys Gln   1 5 10 15 Pro Leu Pro Lys Arg Met Phe              20 <210> 221 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 221 Leu Ala Thr Ser Phe Leu Gln His Pro Trp Glu Glu Asn Arg Asn Asp   1 5 10 15 Tyr Leu Ser Asn Ser Ala Asn              20 <210> 222 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 222 Tyr Ser Leu Arg Lys Leu Ser Lys Ile Leu Ser Thr Thr Lys Glu Glu   1 5 10 15 Asp Ala Ala Cys Ser Ala Ser              20 <210> 223 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 223 Pro Arg Asp Ser Thr Val Thr Asn Gly Asp Asp Lys Gln Ser Ser Lys   1 5 10 15 Asn Ala Val Gln Gly Lys Arg              20 <210> 224 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 224 Ala Leu Gln Leu Ser Asn Ser Asp Gly Lys Pro Glu Pro Lys Ser Ser   1 5 10 15 Glu Ala Leu Glu Asn Met Phe              20 <210> 225 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 225 Val Lys Gly Asn Ser Asn Asn Asn Asn Asn Tyr Arg Arg Arg Asn Arg   1 5 10 15 Arg Gly Gly Arg Gln Ser Val              20 <210> 226 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 226 Thr Pro Ser Pro Asn Lys Pro Arg Met His Arg Lys Arg Asn Ser Arg   1 5 10 15 Gln Ser Leu Gly Ala Thr Pro              20 <210> 227 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 227 Leu Leu Leu Leu Phe Ala Phe Phe Ala Thr Tyr Glu Ala Lys Glu Tyr   1 5 10 15 Glu Arg Ile Ile Lys Glu Leu              20 <210> 228 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 228 Gly Val Val Leu Phe Asn Arg Thr Asp Thr Glu Val Gln Ser Glu Gln   1 5 10 15 Pro Ile Pro Pro Arg His Pro              20 <210> 229 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 229 Val Phe Pro Asp Ser Pro Ile Ala Lys Tyr Arg Pro Pro Gln Ser Pro   1 5 10 15 Ala Arg Pro Leu Arg Asn Asp              20 <210> 230 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 230 Pro Gln Ser Pro Ala Arg Pro Leu Arg Asn Asp Thr Lys Glu His Asn   1 5 10 15 Pro Cys Ala Lys Asp Glu Ser              20 <210> 231 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 231 Arg Ala Asp Asp Thr Trp Gln Leu Ile Val Asn Asn Asp Glu Tyr Lys   1 5 10 15 Gln Ala Ile Gln Ile Glu Glu              20 <210> 232 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 232 Phe Pro Gln Ser Tyr Asn Pro Ile Cys Lys Gln His Tyr Thr Gln Gln   1 5 10 15 Thr Leu Ala Ser Ile Lys Ser              20 <210> 233 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 233 Val Leu Leu Leu Leu Phe Ala Phe Phe Ala Thr Asp Glu Ser Gln His   1 5 10 15 Leu Arg Asn Phe Cys Thr Asn              20 <210> 234 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 234 Asn Asp Leu Gln Pro Thr Asp Val Ser Ser Arg Val Gly Gly Ser Asp   1 5 10 15 Glu Arg Phe Leu Cys Arg Ser              20 <210> 235 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 235 Asn Ile Asn Ser Asn Ser Ser His Ser Ser Asn Asn Asn Asn Asn Gly   1 5 10 15 Ser Ser Ser Asn Asn Asp Asn              20 <210> 236 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 236 Asn Asn Asn Asn Gly Ser Ser Ser Asn Asn Asp Asn Asn Ser Asn Asp   1 5 10 15 Asp Ala Ala Ser Ser Ser Asn              20 <210> 237 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 237 Ala Ala Ser Ser Ser Asn Ser Lys Asn Asn Asn Thr Ser Asn Glu Ser   1 5 10 15 Ser His Ser Asn Asn Asn Thr              20 <210> 238 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 238 Ser Arg Thr Thr Thr Pro Thr Thr Thr Thr Thr Thr Pro Ser Ser Ser Ser   1 5 10 15 Ser Thr Ala Ser Asn Gly Phe              20 <210> 239 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 239 Asp Gly Lys Phe Ile Leu Glu Leu Ala Arg Ser Lys Asp Gly Asp Lys   1 5 10 15 Ser Gly Trp Val Ser Val Thr              20 <210> 240 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 240 Thr Pro Thr Ser Ala Val Thr Thr Ala Tyr Pro Lys Asn Glu Asn Ser   1 5 10 15 Thr Ser Leu Ser Phe Ser Asp              20 <210> 241 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 241 Gln Ser Ser Pro Trp Gln Arg Asp Gln Pro Trp Lys Gln Ser Arg Pro   1 5 10 15 Arg Arg Gly Ile Ser Lys Glu              20 <210> 242 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 242 Thr Leu Gly Arg Ala Ala Leu Arg Thr Ala Ala Arg Lys Arg Arg Arg   1 5 10 15 Pro His Glu Pro Leu Thr Thr              20 <210> 243 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 243 Thr Ile Lys Gly Glu Asp Asp Ala Glu Arg Leu Glu Lys Glu Pro Lys   1 5 10 15 Lys Ala Val Ser Asp Asp Ser              20 <210> 244 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 244 Ser Lys Glu Ala Ser Pro Gly Gln Gln Val Glu Pro Gln Pro Lys Asp   1 5 10 15 Glu Thr Val Asp Val Glu Met              20 <210> 245 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 245 Leu Asn Gly Asp Leu Lys Ala Ser Ile Gly Lys Pro Lys Ser Lys Pro   1 5 10 15 Lys Pro Lys Ala Lys Leu Ser              20 <210> 246 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 246 Leu Ala Pro Pro Gln Gly Lys Pro Ser Pro Ser Pro Gly Ser Ser Ser   1 5 10 15 Ser Ser Thr Ser Pro Ala Thr              20 <210> 247 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 247 Gly Gly Gly Lys Ser Pro Ser His Thr Gly Thr Lys Lys Arg Ser Pro   1 5 10 15 Pro Tyr Ser Ala Gly Ser Pro              20 <210> 248 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 248 Pro Ser Pro Gly Asp Arg Ser Ala Ser Pro Pro Glu Arg Arg His Met   1 5 10 15 Gln Gln Gln Pro His Leu Gln              20 <210> 249 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 249 Ser Ser Ser Ala Met Phe His Thr Ser Ser Leu Arg Asn Glu Gln Ser   1 5 10 15 Ser Asp Leu Pro Leu Asn Leu              20 <210> 250 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 250 Ser Val Ala Glu Cys Asn Arg Gln Thr Thr Met Thr Asp Glu His Lys   1 5 10 15 Ser Asn Ile Asn Ser Asn Ser              20 <210> 251 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 251 Ser Asn Ser Ser His Ser Ser Asn Asn Asn Asn Asn Gly Ser Ser Ser   1 5 10 15 Asn Asn Asp Asn Asn Ser Asn              20 <210> 252 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 252 Asn Ser Asn Asp Asp Ala Ala Ser Ser Ser Asn Ser Lys Asn Asn Asn   1 5 10 15 Thr Ser Asn Glu Ser Ser His              20 <210> 253 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 253 Leu Ser Arg Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Ser Ser Ser Ser   1 5 10 15 Ser Ser Thr Ala Ser Asn Gly              20 <210> 254 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 254 Thr Val Thr Pro Thr Ser Ala Val Thr Thr Ala Tyr Pro Lys Asn Glu   1 5 10 15 Asn Ser Thr Ser Leu Ser Phe              20 <210> 255 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 255 Gln Ser Ser Pro Trp Gln Arg Asp Gln Pro Trp Lys Gln Ser Arg Pro   1 5 10 15 Arg Arg Gly Ile Ser Lys Glu              20 <210> 256 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 256 Arg Gly Ile Ser Lys Glu Leu Ser Leu Phe Phe His Arg Pro Arg Asn   1 5 10 15 Ser Thr Leu Gly Arg Ala Ala              20 <210> 257 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 257 Leu Gly Arg Ala Ala Leu Arg Thr Ala Ala Arg Lys Arg Arg Arg Pro   1 5 10 15 His Glu Pro Leu Thr Thr Ser              20 <210> 258 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 258 Thr Ile Lys Gly Glu Asp Asp Ala Glu Arg Leu Glu Lys Glu Pro Lys   1 5 10 15 Lys Ala Val Ser Asp Asp Ser              20 <210> 259 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 259 Glu Ser Lys Glu Ala Ser Pro Gly Gln Gln Val Glu Pro Gln Pro Lys   1 5 10 15 Asp Glu Thr Val Asp Val Glu              20 <210> 260 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 260 Asp Leu Asn Gly Asp Leu Lys Ala Ser Ile Gly Lys Pro Lys Ser Lys   1 5 10 15 Pro Lys Pro Lys Ala Lys Leu              20 <210> 261 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 261 Ala Pro Pro Gln Gly Lys Pro Ser Pro Ser Pro Gly Ser Ser Ser Ser   1 5 10 15 Ser Thr Ser Pro Ala Thr Leu              20 <210> 262 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 262 Asn Ser Ser Tyr Ser Ile His Ser Leu Leu Gly Gly Ser Ser Gly Ser   1 5 10 15 Gly Ser Ser Ser Phe Ser Ser              20 <210> 263 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 263 Gly Gly Lys Ser Pro Ser His Thr Gly Thr Lys Lys Arg Ser Pro Pro   1 5 10 15 Tyr Ser Ala Gly Ser Pro Val              20 <210> 264 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 264 Ser Pro Gly Asp Arg Ser Ala Ser Pro Pro Glu Arg Arg His Met Gln   1 5 10 15 Gln Gln Pro His Leu Gln Arg              20 <210> 265 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 265 Ser Ser Ala Met Phe His Thr Ser Ser Leu Arg Asn Glu Gln Ser Ser   1 5 10 15 Asp Leu Pro Leu Asn Leu Ser              20 <210> 266 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 266 Arg Val Thr Thr Cys Phe Gln Gln Leu Arg Asp Lys Leu Gln Ser Ser   1 5 10 15 Gly Ser Phe Arg Lys Ser Ser              20 <210> 267 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 267 Ser Ala Cys Gly Glu Arg Phe Arg Lys Ile Phe Lys Ser Ala Arg Lys   1 5 10 15 Thr Glu Leu Pro Glu Leu Trp              20 <210> 268 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 268 Trp Lys Val Pro Leu Val Ala His Glu Leu Thr Ser Arg Gln Ser Ser   1 5 10 15 Gln Gln Leu Gln Val Val Ala              20 <210> 269 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 269 Gln Ile Ser Thr Lys Glu Ile Thr Tyr Asn Ser Asn Ser Asn Thr Ser   1 5 10 15 Glu Asn Asn Met Thr Ile Ile              20 <210> 270 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 270 Lys Asn Gly His Leu Leu Asp Met Val Leu Asn Gln Lys Glu Arg Thr   1 5 10 15 Ser Asp Tyr Ser Ser Gly Ala              20 <210> 271 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 271 Ala Pro Ser Leu Glu Asn Ile Pro Arg Ala Pro Pro Arg Tyr Trp Lys   1 5 10 15 Asn Pro Phe Lys Arg Arg Ala              20 <210> 272 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 272 Leu Asn Thr Ser Pro Arg Thr Val Pro Lys Ile Thr Asp Glu Lys Thr   1 5 10 15 Lys Asp Ile Ala Thr Arg Pro              20 <210> 273 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 273 Pro Lys Met Pro Val Pro Ile Asn Ile Tyr Ser Pro Asp Ala Pro Glu   1 5 10 15 Glu Pro Ile Asn Leu Ala Pro              20 <210> 274 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 274 Pro Pro Gly His Gln Pro Ser Cys Arg Thr Gln Ser Gln Lys Thr Glu   1 5 10 15 Pro Thr Glu Asn Arg His Leu              20 <210> 275 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 275 Val His Pro Phe Asn Gly Met Asn Ile Met Lys Arg Arg His Glu Met   1 5 10 15 Thr Pro Thr Pro Thr Ile Leu              20 <210> 276 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 276 Asn Pro Ser Leu Val Ile Asp Val Tyr Gly Thr Arg Arg Gly Pro Lys   1 5 10 15 Ser Lys Asn Gly Gly Pro Ile              20 <210> 277 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 277 Pro Lys Ser Lys Asn Gly Gly Pro Ile Ala Ser Gly Gly Ser Gly Ser   1 5 10 15 Gly Ser Arg Lys Ser Asn Val              20 <210> 278 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 278 Ser Arg Lys Ser Asn Val Ala Ala Val Ala Pro Gln Gln Gln Gln Ser   1 5 10 15 Gln Pro Ala Gln Pro Val Ala              20 <210> 279 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 279 Gln Leu Ser Pro Arg Met Arg Glu Gln Leu Gln Gln Gln Asn Gln Gln   1 5 10 15 Gln Ser Asp Asn Glu Glu Glu              20 <210> 280 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 280 Gln Gln Gln Ser Asp Asn Glu Glu Glu Glu Gln Asp Asp Glu Tyr Glu   1 5 10 15 Arg Lys Ser Val Asp Ser Ala              20 <210> 281 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 281 Ala Met Asp Leu Ser Gln Gly Thr Pro Val Lys Glu Asp Glu Gln Gln   1 5 10 15 Gln Gln Pro Gln Gln Pro Leu              20 <210> 282 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 282 Ser Ser Asn Ala Ser Ser Asn Ser Asn Gly Asn Ser Ser Ser Asn Ser   1 5 10 15 Ser Ser Asn Gly Thr Thr Ser              20 <210> 283 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 283 Met Thr Ser Lys Lys Arg Lys Thr Leu Leu Asp Ala Asp Asp   1 5 10 <210> 284 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 284 Leu Asp Ala Asp Asp Asp Asn Asp Asn Phe Asp Glu Asp Asp Ser Gly   1 5 10 15 Ser Asp Phe Asp Asp Asp Glu              20 <210> 285 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 285 Asn Phe Asp Glu Asp Asp Ser Gly Ser Asp Phe Asp Asp Asp Glu Asp   1 5 10 15 Pro Asp Gln Ile Glu Val Pro              20 <210> 286 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 286 Asn Asn Asn Asn Asn Asn Asn Ile Val Thr Ser Ser Val Asn Asn Arg Asn Asn   1 5 10 15 Asn Asn Ile Ile Ser Thr Asn              20 <210> 287 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 287 Asn Asn Arg Asn Asn Asn Asn Ile Ile Ser Thr Asn Gly Ser Ser Asn   1 5 10 15 Asn Asn Asn Asn Ser Ile Asn              20 <210> 288 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 288 Ala Ala Ala Ala Ala Ala Ala Ala Ala Leu Gln Gln Gln Gln Gln Gln   1 5 10 15 Gln Ser Pro Leu His Ser Pro              20 <210> 289 <211> 25 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 289 Ser Asn Asn Ser His Gln Asp Glu Glu Glu Leu Asp Asp Glu Glu Glu   1 5 10 15 Asp Glu Glu Glu Asp Glu Asp Glu Asp              20 25 <210> 290 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 290 Glu Leu Asp Asp Glu Glu Glu Asp Glu Glu Glu Asp Glu Asp Glu Asp   1 5 10 15 Asp Glu Glu Glu Asn Ala Ser              20 <210> 291 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 291 Gln Glu Thr Arg Thr Glu Pro Ser Ala Thr Thr Gln Gln Gln His Gln   1 5 10 15 Gln Gln Asp Thr Glu Asp Leu              20 <210> 292 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 292 Thr Ala Thr Ser Asn Thr Asn Asn Asn Asn Asn Asn Asn Ser Ser Ser   1 5 10 15 Gly Asn Ser Glu Lys Arg Lys              20 <210> 293 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 293 Asn Asn Asn Asn Asn Ser Ser Ser Gly Asn Ser Glu Lys Arg Lys Lys Lys   1 5 10 15 Asn Asn Asn Asn Asn Asn Gly              20 <210> 294 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 294 Arg Arg Ala Asp Gly Ala Ala Ser Pro Thr Pro Ser Asp Ser Ser Asp   1 5 10 15 Ser Asp Ile Ser Leu Gly Thr              20 <210> 295 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 295 Ser Pro Val Pro Ser Ser Leu Gln Leu Gln His Ser Pro Gly Ser Thr   1 5 10 15 Ser Asn Gly Ala Asn Asp Arg              20 <210> 296 <211> 24 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 296 Gly Ala Asn Asp Arg Glu Glu Ser Leu Ser Val Asp Asp Asp Lys Pro   1 5 10 15 Arg Asp Leu Ser Gly Ser Leu Pro              20 <210> 297 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 297 Asn Ser Thr Ala Val Ala Ala Ala Ala Ser Val Gly Gly Gly Gly Gly   1 5 10 15 Asn Gln His His Glu Pro Thr              20 <210> 298 <211> 17 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 298 Met Ala Asp Val Ala Glu Gln Lys Asn Glu Thr Pro Val Val Glu Lys   1 5 10 15 Val <210> 299 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 299 Glu Pro Thr Val Ser Phe Ala Ala Asp Lys Asp Glu Lys Lys Asp Glu   1 5 10 15 Asp Lys Lys Glu Asp Ser Ala              20 <210> 300 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 300 Lys Lys Glu Asp Ser Ala Ala Asp Gly Glu Asp Thr Lys Lys Glu Ser   1 5 10 15 Ser Glu Ala Val Leu Pro Ala              20 <210> 301 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 301 Lys Glu Ser Ser Glu Ala Val Leu Pro Ala Val Glu Asn Gly Ser Glu   1 5 10 15 Glu Val Thr Asn Gly Asp Ser              20 <210> 302 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 302 Cys Phe Gly Gly Lys Ser Ile Lys Thr Pro Thr Asn Glu Thr Ser Asp   1 5 10 15 Thr Glu Ser Ala Asp Ile Lys              20 <210> 303 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 303 Val Ser Ala Glu Glu Glu Glu Thr Val Asn Glu Gln Glu Ser Pro Gln   1 5 10 15 Glu Gln Gln Gln Gln Gln Gln              20 <210> 304 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 304 Thr Val Asn Glu Gln Glu Ser Pro Gln Glu Gln Gln Gln Gln Gln Gln   1 5 10 15 Lys Lys Ala Lys Arg Leu Pro              20 <210> 305 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 305 Ala Ile Leu Arg Arg Phe Asn Glu Lys Asp Ser Arg Lys Tyr Lys Asp   1 5 10 15 Asn Gln Glu Ser Leu Asn Ile              20 <210> 306 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 306 Gln Glu Ile Pro Ala Thr Asn Asn Ala Ile Asp Gly Asn Asp Asn Glu   1 5 10 15 Pro Lys Ala Ile Val Trp Gln              20 <210> 307 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 307 Asp Glu Pro Leu Asn Asn Thr Tyr Ile Glu Ser Pro Pro Glu Ser Ser   1 5 10 15 Asp Arg Glu Val Ser Pro Lys              20 <210> 308 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 308 Pro Lys Leu Val Val Gly Thr Lys Lys Tyr Gly Arg Arg Ser Arg Pro   1 5 10 15 Gln Gln Gly Ile Tyr Glu Leu              20 <210> 309 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 309 Gln Gln Gly Ile Tyr Glu Leu Ser Val Thr Asp Ser Asp Asn Thr Asp   1 5 10 15 Glu Asp Gln Leu Gln Gln Gln              20 <210> 310 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 310 Asp Ser Asp Asn Thr Asp Glu Asp Gln Leu Gln Gln Gln Gln Asn Gln   1 5 10 15 Arg Ser Leu Asn Gly Cys Asp              20 <210> 311 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 311 Asn Gly Cys Asp Glu Leu Gly Val Gln Val Gln Arg Ser Ser Ser Gln   1 5 10 15 Ser Asp Leu Pro Gly Ser Arg              20 <210> 312 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 312 Ser Lys Leu Lys Arg Cys Ala Ser Leu Pro Ala Arg Arg Lys Met Asn   1 5 10 15 Ser Asn Thr Thr Gly Ala Thr              20 <210> 313 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 313 Pro Gln Leu Gln Arg Ile Gln Pro Tyr Leu Val Pro Gln Arg Asn Arg   1 5 10 15 Ser Gln Ala Gly Gly Leu Leu              20 <210> 314 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 314 Pro Ser Leu Leu Ser Ser Ala Leu Met Pro Lys Arg Lys Arg Gly Arg   1 5 10 15 Pro Arg Lys Leu Ser Gly Ser              20 <210> 315 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 315 Gly Lys Met Cys Asn Glu Ser Tyr Ser Gly Asn Asp Asp Gly Ser Asp   1 5 10 15 Asp Asn Gln Pro Asn Ala Gly              20 <210> 316 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 316 Asp Leu Asn Glu Ser Arg Asp Ser Leu Pro Ser Lys Arg Ser Lys Asn   1 5 10 15 Ser Lys Asp His Arg Val Val              20 <210> 317 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 317 Lys Asn Ser Lys Asp His Arg Val Val Ser His His Glu Asp Asn Ser   1 5 10 15 Thr Ser Asp Gly Asn Asp Ser              20 <210> 318 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 318 Val Val Ser His His Glu Asp Asn Ser Thr Ser Asp Gly Asn Asp Ser   1 5 10 15 Asp Gly Glu Gly Leu Asp Thr              20 <210> 319 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 319 Ala Gly Ser Phe Ser Thr Ala Thr Val Lys Pro Arg Val Gln Lys Arg   1 5 10 15 Pro Lys Leu Gly Lys Gln Asn              20 <210> 320 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 320 Ser Leu Pro Ala Thr Ser Asn Ala Leu Ser Thr Ser Ser Ser Ser Ser   1 5 10 15 Thr Ser Ser Ser Ser Gly Ser              20 <210> 321 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 321 Ser Leu Val Gly Gln Glu His Ile Val Met Pro Glu Ser Ser Asp Ser   1 5 10 15 Asn Thr Leu Cys Pro Ile Ser              20 <210> 322 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 322 Glu Pro Leu Thr Asn Ser Asp Gly Cys Ser Glu Asp Asn Asn Lys Glu   1 5 10 15 Pro Glu Lys Ser Asn Ser Ser              20 <210> 323 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 323 Cys Ser Glu Asp Asn Asn Lys Glu Pro Glu Lys Ser Asn Ser Ser Gln   1 5 10 15 Ser Ser Asp His Ile Ala Ser              20 <210> 324 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 324 Pro Ser Gly Gly Ser Ser Leu Leu Asn Ile Asn Pro Ser Glu Asn Arg   1 5 10 15 Ser Asp Val Glu Leu Asn Leu              20 <210> 325 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 325 Gly Ser Gly Leu Ile Asn Ser Ser Tyr Tyr Tyr Glu Ser Gly Gln Thr   1 5 10 15 Gln Ser Pro Leu Thr His Asp              20 <210> 326 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 326 Asn Leu Asn Gly Asp Gly Ser Ala Ala Gln Ser Thr Ser Gln Ser Gln   1 5 10 15 Ser Gln Ser Pro Ala Ala Thr              20 <210> 327 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 327 Asp Ala Gly Val Pro Pro Leu Pro Leu Pro Leu Pro Gln Ser Thr Pro   1 5 10 15 Gln Pro Leu Leu Met Ala Leu              20 <210> 328 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 328 Ala Met Gly Val Ile Pro Ser Thr Asn Asp Ser Asn Asn Asn Asn Asn   1 5 10 15 Asn Asn Asn Asn Val Asp Asn              20 <210> 329 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 329 Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Val Asp Asn Thr Ser Asp Glu   1 5 10 15 Ser Asn Pro Val Thr Ile Tyr              20 <210> 330 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 330 Leu Glu Leu Gln Arg Lys Gln Leu Glu Ala Ser Ala Gln Glu Glu Ala   1 5 10 15 Asp Gln Leu His Pro Asp Thr              20 <210> 331 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 331 Ala Gln Glu Glu Ala Asp Gln Leu His Pro Asp Thr Asp Pro Asn Pro   1 5 10 15 Asp Ser Gly Gly Gln Leu Pro              20 <210> 332 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 332 Glu Thr Val Thr His Thr Gly Glu Pro Pro Pro Asp Pro Ser Ser Ser   1 5 10 15 Ser Thr Pro Asp Ser Thr Thr              20 <210> 333 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 333 Ser Ser Ser Thr Pro Asp Ser Thr Thr Pro Ser Pro Asn Asp Lys Glu   1 5 10 15 Thr Glu Ile Gln Met Leu Pro              20 <210> 334 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 334 Ala Ala Val Phe Met Leu Arg Asn Val Pro Asp Tyr Arg Arg Lys Gln   1 5 10 15 Gln Gln Leu His Leu His Lys              20 <210> 335 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 335 Thr Thr Ser Ala Gly Asn Arg Arg Gln His Arg Tyr Arg Pro Arg Lys   1 5 10 15 Leu Ile Lys Ser Asp              20 <210> 336 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 336 Gln His Arg Tyr Arg Pro Arg Lys Leu Ile Lys Ser Asp Asp Glu Asp   1 5 10 15 Asp Asp Ser Asn Ser Glu Lys              20 <210> 337 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 337 Ser His His His Gln Ser Gln Ser Ala Arg Tyr His Gln Lys Asn Asn   1 5 10 15 Ser Arg Ala Glu Gln Cys Cys              20 <210> 338 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 338 Ala Thr Gln Arg Val Lys Met Leu Arg Arg Ala Arg Lys Gln Lys Tyr   1 5 10 15 Gln Asp His Cys Leu Glu Thr              20 <210> 339 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 339 Ser Leu Thr Pro His Gln His Gln Gln Gln Asn Gln Gln Gln Gln Gln   1 5 10 15 Gln Gln Arg Lys Ser Ala Glu              20 <210> 340 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 340 Pro Ile Arg Gln Pro Ala Ala Gln Gln Gln Pro Gln Gln Gln Gln Arg   1 5 10 15 Gln Arg Cys Asn Thr Gly Ser              20 <210> 341 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 341 Lys Asn Leu Leu Gln Gly Tyr Gln Arg Leu Glu Gln Ser Gln Gln Gln   1 5 10 15 Gln Gln Gln Gln Arg Ser Ser              20 <210> 342 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 342 Tyr Ala Lys Leu Ser Glu Gln Leu Gln Gln Lys Asp Arg Glu Gln Arg   1 5 10 15 Arg Gln Arg His Lys Gln              20 <210> 343 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 343 Gln Met Leu Gln Asp Gln Pro Lys Asp Ala Ser Arg Ser Glu Gln Arg   1 5 10 15 Pro Pro Thr Ser Asn Ser Ser              20 <210> 344 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> peptide <400> 344 Phe Arg Gly Ser Pro Leu Lys Val Lys Arg Ser Asp   1 5 10

Claims (11)

(a) 소에서 발현되는 단백질 서열과, 양서류, 곤충, 파충류, 어류 및 식물로 이루어진 군으로 선택되는 생물에서 발현되는 단백질 서열간의 상동성을 분석하는 단계;(a) analyzing homology between protein sequences expressed in cattle and protein sequences expressed in organisms selected from the group consisting of amphibians, insects, reptiles, fish and plants; (b) 상기 분석결과로부터, 상기 생물에서만 특이적으로 발현되는 단백질 서열을 선별하는 단계;(b) selecting a protein sequence specifically expressed in the organism from the analysis result; (c) 상기 선별한 단백질 서열에서 항원성 부위를 검색하고, 상기 항원성 부위를 포함하는 펩타이드를 제작하는 단계; 및(c) searching for an antigenic site in the selected protein sequence and preparing a peptide comprising the antigenic site; And (d) 상기 펩타이드를 소에 주입하여 면역화시키는 단계(d) immunizing the peptide by injecting it into a cow 를 포함하는 브랜드 소의 제조방법.Brand cow manufacturing method comprising a. 제 1항에 있어서, 상기 (a) 단계에서 상기 생물은, 개구리, 바퀴벌레, 초파리, 애기장대(Arabidopsis) 및 영원(Triturus)으로 이루어진 군으로부터 선택되는 것인 방법.The method of claim 1, wherein in the step (a), the organism is selected from the group consisting of frogs, cockroaches, fruit flies, Arabidopsis, and Tritus. 제 1항에 있어서, 상기 (c) 단계의 펩타이드는 서열번호 1 내지 344로 기재된 펩타이드로 이루어진 군으로부터 선택되는 것인 방법.The method of claim 1, wherein the peptide of step (c) is selected from the group consisting of the peptides set forth in SEQ ID NOs: 1 to 344. 제 1항에 있어서, 상기 (d) 단계에서 면역은, 1회 면역 또는 2 내지 4회의 연속된 면역인 것인 방법.The method of claim 1, wherein the immunization in step (d) is one immunization or two to four consecutive immunizations. 제 1항에 있어서, 상기 (d) 단계의 주입은 경구투여 또는 주사주입인 방법.The method of claim 1, wherein the infusion of step (d) is oral or injection. 제 1항에 있어서, 상기 (d) 단계의 상기 펩타이드는 합성 펩타이드, 천연 펩타이드 또는 유전공학적인 방법으로 제조된 재조합 펩타이드인 방법.The method of claim 1, wherein the peptide of step (d) is a synthetic peptide, natural peptide or recombinant peptide prepared by genetic engineering method. 제 1항에 있어서, 상기 (d) 단계의 상기 펩타이드는 보강제(Adjuvant)와 동시에 주입되는 것인 방법.  The method of claim 1, wherein the peptide of step (d) is injected simultaneously with an adjuvant. 제 1항에 있어서, 상기 (d) 단계의 상기 펩타이드는 펩타이드 N-말단 또는 C-말단에 운반체 단백질이 더욱 접합된 것인 방법.The method of claim 1, wherein the peptide of step (d) is a carrier protein further conjugated to the peptide N-terminus or C-terminus. 제 1항에 있어서, 상기 고기는 육즙 및 혈청에 상기 펩타이드에 대한 항체가 포함된 것인 방법.The method of claim 1, wherein the meat comprises an antibody against the peptide in gravy and serum. 제 1항 내지 9항의 방법으로 제조된 브랜드 소.A brand cow prepared by the method of claims 1-9. (a) 소에서 발현되는 단백질 서열과, 양서류, 곤충, 파충류, 어류 및 식물류로 이루어진 군으로 선택되는 생물에서 발현되는 단백질 서열간의 상동성을 분석 하는 단계; (a) analyzing homology between protein sequences expressed in cattle and protein sequences expressed in organisms selected from the group consisting of amphibians, insects, reptiles, fish and plants; (b) 상기 분석결과로부터, 상기 생물에서만 특이적으로 발현되는 단백질 서열을 선별하는 단계;(b) selecting a protein sequence specifically expressed in the organism from the analysis result; (c) 상기 선별한 단백질 서열에서 항원성 부위를 검색하고, 상기 항원성 부위를 포함하는 펩타이드를 제작하는 단계; (c) searching for an antigenic site in the selected protein sequence and preparing a peptide comprising the antigenic site; (d) 상기 펩타이드를 소에 주입하여 면역화시키는 단계;(d) immunizing the peptide by injecting it into a cow; (e) 상기 소 또는 이의 일부로부터 육즙 또는 혈청을 수득한 후, 이를 상기 (d) 단계의 펩타이드와 반응시켜 면역반응이 형성됨을 확인하는 단계를 포함하는 브랜드 쇠고기의 식별방법. (e) obtaining broth or serum from the cow or a portion thereof, and then reacting it with the peptide of step (d) to confirm that an immune response is formed.
KR1020040050473A 2004-06-30 2004-06-30 Preparation method for brand meat and identification method same KR20060001369A (en)

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