KR20040064006A - Bactericide and insecticide composition comprising chitosan and pepper solution and Method thereof - Google Patents

Bactericide and insecticide composition comprising chitosan and pepper solution and Method thereof Download PDF

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KR20040064006A
KR20040064006A KR1020030000820A KR20030000820A KR20040064006A KR 20040064006 A KR20040064006 A KR 20040064006A KR 1020030000820 A KR1020030000820 A KR 1020030000820A KR 20030000820 A KR20030000820 A KR 20030000820A KR 20040064006 A KR20040064006 A KR 20040064006A
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South Korea
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chitosan
red pepper
stock solution
insecticide composition
purified water
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KR1020030000820A
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Korean (ko)
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김진홍
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김진홍
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Publication of KR20040064006A publication Critical patent/KR20040064006A/en

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    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16H—GEARING
    • F16H1/00—Toothed gearings for conveying rotary motion
    • F16H1/28—Toothed gearings for conveying rotary motion with gears having orbital motion
    • F16H1/32—Toothed gearings for conveying rotary motion with gears having orbital motion in which the central axis of the gearing lies inside the periphery of an orbital gear
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16D—COUPLINGS FOR TRANSMITTING ROTATION; CLUTCHES; BRAKES
    • F16D3/00—Yielding couplings, i.e. with means permitting movement between the connected parts during the drive
    • F16D3/16—Universal joints in which flexibility is produced by means of pivots or sliding or rolling connecting parts
    • F16D3/26—Hooke's joints or other joints with an equivalent intermediate member to which each coupling part is pivotally or slidably connected
    • F16D3/30—Hooke's joints or other joints with an equivalent intermediate member to which each coupling part is pivotally or slidably connected in which the coupling is specially adapted to constant velocity-ratio
    • F16D3/32—Hooke's joints or other joints with an equivalent intermediate member to which each coupling part is pivotally or slidably connected in which the coupling is specially adapted to constant velocity-ratio by the provision of two intermediate members each having two relatively perpendicular trunnions or bearings
    • F16D3/33—Hooke's joints or other joints with an equivalent intermediate member to which each coupling part is pivotally or slidably connected in which the coupling is specially adapted to constant velocity-ratio by the provision of two intermediate members each having two relatively perpendicular trunnions or bearings with ball or roller bearings

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  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

PURPOSE: Provided is a fungi-insecticide composition which completely exterminates various kinds of pathogenic bacteria and injurious insects in an environment friendly manner while complementing minerals and vitamins to the crops, and exerts extraordinary fungicidal and insecticidal effects with respect to the lawn grass. CONSTITUTION: The fungi-insecticide composition comprises 20-40wt.% of chitosan diluent, 10-30wt.% of hot pepper extract, and 40-60wt.% of purified water. The chitosan diluent is made by diluting a chitosan extract by 1800-2200 times. The hot pepper extract contains hot pepper, liquor with an alcoholic degree of 35-40, and purified water.

Description

키토산과 고추액을 포함하는 살균ㆍ살충제 조성물 및 그 제조방법{Bactericide and insecticide composition comprising chitosan and pepper solution and Method thereof}Bactericide and insecticide composition comprising chitosan and pepper solution and Method

본 발명은 키토산 희석액과 고추원액을 포함하는 살균ㆍ살충제 조성물 및 그 제조방법에 관한 것이다.The present invention relates to a bactericidal / insecticide composition comprising a chitosan diluent and a red pepper stock solution and a method for producing the same.

키토산은 키틴 유도체의 하나로 키틴(Chitin)으로부터 얻어지는 폴리머이다.키틴은 하등생물의 껍데기를 구성하는 자연계에 존재하는 천연 동물성 섬유질로서 년간 1000억톤 이상이 생산되고 있으며, N-아세틸-글루코사민(N-Acetyl Glucosamine) 이라는 당이 β1-4 형태로 결합한 분자량 100만 이상의 고분자 물질이다. 키토산(Chitosan)은 키틴을 탈아세틸화 반응을 거쳐 키틴의 거대분자가 저분자화된 형태라고 할 수 있다. 이렇게 만들어진 키토산은 물, 알코올 용매에는 녹지 않으며 약산에 녹는 특성을 갖고 있으며, 무공해·무독성의 생분해성 물질이다.Chitosan is one of the chitin derivatives and is a polymer obtained from chitin. Chitin is a natural animal fiber that exists in nature that constitutes the shell of lower organisms. It is produced over 100 billion tons per year, and N-Acetyl Glucosamine) is a high molecular weight polymer with a molecular weight of 1 million or more in the form of β1-4. Chitosan is a form of low molecular weight of chitin's macromolecules through deacetylation of chitin. The chitosan made in this way is insoluble in water and alcohol solvents, has a characteristic of being soluble in weak acid, and is a pollution-free and non-toxic biodegradable substance.

게나 새우를 퇴비로 쓰는 경우 토양의 지력(地力)이 강화된다는 사실이 농부들의 경험에서 인정되어 왔다. 그리고 키틴이나 키토산의 저분자물과 올리고당들은 병원균과 병해충에 대하여 상당한 통제력이 있음이 여러 실험에서 밝혀져 있다. 키틴이나 키토산은 토양중의 미생물 가운데 식물에 해로운 위루스균, 사상균(絲狀菌)을 억제하고 식물에 유익한 방선균(放線菌)을 증식시킨다는 사실이 밝혀졌다. 그러나 특히 고추 작물의 역병, 탄저병, 세균성 점무늬병등에 대해서 강력한 살균, 살충력이 필요한데 키토산만으로는 완벽하게 이들 병원균을 박멸할 수 없다. 또한 동일한 식물이 동일한 병에 걸리더라도 토양과 기후에 따라 병원균의 종류가 달라지기 때문에 키토산의 효과가 일정치 않게 되는 문제가 있었다.The use of crabs and shrimp for composting has strengthened the soil's intellect, which has been recognized by farmers' experience. And several experiments have shown that low molecular weight and oligosaccharides of chitin and chitosan have significant control over pathogens and pests. Chitin and chitosan have been found to inhibit harmful bacteria of plants, filamentous fungi, and actinomycetes, which are beneficial to plants. However, especially strong pesticides and insecticides against pestilence, anthrax and bacterial spot disease of pepper crops, chitosan alone cannot completely eradicate these pathogens. In addition, even if the same plants get the same disease, the effect of chitosan was not constant because the types of pathogens vary depending on soil and climate.

고추는 각종 비타민이 풍부하고 알카로이드의 일종인 캡사이신이 매운맛을 내므로 살충작용을 하고 감염에 대한 저항력과 면역력을 증진시키는 약리작용이 있다. 따라서 이를 이용한 병충해의 예방 및 박멸에 대한 가능성이 제시되어 왔다. 그 예로서 대한민국 특허공개공보 제 2002-0035184호에는 고추즙액 혹은 고추분말을 포함하는 농약이 개시되어 있다. 그러한 상기 공보에는 단순히 고추의 살균효과를 이용하기 위하여 고추성분을 첨가한 농약을 개시하고 있을 뿐, 구체적으로 고추즙액 제조방법에 대하여 언급되어 있지 않다. 뿐만 아니라, 이러한 고추의 살균, 살충력을 상승시키거나, 지속시키는 방법에 대해 전혀 개시된 바가 없다.Red pepper is rich in various vitamins and is a kind of alkaloid capsaicin that gives a spicy taste, it acts as a pesticide and has a pharmacological action to increase resistance to infection and immunity. Therefore, the possibility of the prevention and eradication of pests using this has been suggested. As an example, Korean Patent Laid-Open Publication No. 2002-0035184 discloses a pesticide containing red pepper juice or red pepper powder. Such a publication merely discloses a pesticide to which pepper components are added in order to take advantage of the bactericidal effect of pepper, and does not specifically mention a method for preparing pepper juice. In addition, there is no disclosure on how to increase or sustain the killing, insecticidal properties of these peppers.

종래의 농약은 화학약품제재로서 맹독성 물질이므로 사용시 인체에 위험한 요소가 많고 사용 후 분해되는데 많은 시일이 걸리기 때문에 잔류하여 부작용을 초래할 뿐아니라 환경오염을 시키는 등의 문제가 있다. 또한 농약은 단순히 살균, 살충의 목적으로만 사용될 뿐 작물의 생장에 필요한 영양분을 보충해 주기위해서는 별도로 비료를 사용하는 것이 일반적이다.Conventional pesticides are highly toxic as chemicals, so there are many risk factors for the human body when using them, and many seals take a long time to decompose after use, resulting in side effects as well as environmental pollution. In addition, pesticides are used only for the purpose of sterilization and insecticide, but it is common to use fertilizer separately to supplement the nutrients necessary for the growth of crops.

특히 골프장 잔디의 병충해를 박멸하기 위해 사용되던 종래의 살충제는 독성이 강하여 토양을 오염시키고 인체에 악영향을 끼치는 것으로 문제된 바 있으며, 이의 해결을 위한 잔디 병충해 살충제의 개발이 시급한 실정이다.In particular, conventional pesticides used to eradicate pests of golf courses have been toxic and have been problematic to contaminate soil and adversely affect the human body, and it is urgent to develop grass pest pesticides for the solution.

또한 바퀴벌레 등에 사용되던 일반 살충제도 화학약품들로서 인체에 해를 끼치는 것이 대부분이고, 따라서 천연제재로 된 살충제의 개발이 요구되어 왔다.In addition, general insecticides used in cockroaches, etc. are mostly chemicals that harm the human body, and therefore, development of natural pesticides has been required.

본 발명은 위와 같은 여러가지 문제점을 해소하기 위하여 키토산과 고추원액을 포함하는 인체에 해가 없으면서 성능이 좋은 살균ㆍ살충제 조성물 및 그 제조 방법을 제공하는데 목적이 있다. 즉, 안전성이 높고 살균ㆍ살충효과가 뛰어난 천연제재의 살균ㆍ살충제 조성물 및 그 제조 방법을 제공하는 것이다.The present invention aims to provide a sterilizing and insecticide composition having good performance without harm to the human body including chitosan and red pepper stock solution in order to solve the above problems. That is, the present invention provides a sterilizing and insecticidal composition of a natural product having high safety and excellent sterilizing and insecticidal effects, and a method for producing the same.

따라서 본 발명은 농작물은 물론, 골프장 잔디, 및 일반 해충의 박멸 등 광범위한 용도로 쓰일 수 있는 천연제재의 살균ㆍ살충제 조성물을 제공할 수 있다.Therefore, the present invention can provide a sterilizing and insecticidal composition of a natural agent that can be used for a wide range of applications such as crops, golf course grass, and general pest extermination.

그리고 본 발명의 살균ㆍ살충제 조성물을 작물에 사용할 경우 토양의 지력을 향상시키고, 작물의 생장 발육에 필요한 양분을 보충하므로 비료로서의 역할을 병행할 수 있다.In addition, when the bactericidal / pesticide composition of the present invention is used in crops, it improves the intellect of the soil and replenishes the nutrients necessary for the growth and development of the crops.

상기 목적을 달성하기 위하여 본 발명의 살균ㆍ살충제 조성물은 키토산 희석액, 고추원액 및 정제수를 포함하는 것을 특징으로 한다.In order to achieve the above object, the bactericidal / insecticide composition of the present invention is characterized in that it contains chitosan diluent, red pepper stock solution and purified water.

상기 발명의 일 실시예는 키토산 희석액 20~40 중량%, 고추원액 10~30중량%, 및 정제수 40~60 중량% 포함하는 것을 특징으로 한다.One embodiment of the present invention is characterized in that it comprises 20 to 40% by weight of chitosan diluent, 10 to 30% by weight of red pepper stock solution, and 40 to 60% by weight of purified water.

상기 발명의 일 실시예는 키토산 희석액이 키토산 원액을 1800 내지 2200 배희석시킨 것임을 특징으로 한다.One embodiment of the invention is characterized in that the dilution of the chitosan dilution 1800 to 2200 times the chitosan stock solution.

상기 발명의 일 실시예는 고추원액이 붉은 고추, 알콜도수 35° 내지 40°인 주류, 및 정제수를 포함하는 것을 특징으로 한다.One embodiment of the present invention is characterized in that the red pepper juice comprises red pepper, alcoholic beverages with 35 ° to 40 ° alcohol, and purified water.

본 발명의 다른 실시예는 키토산 희석액을 제조하는 단계, 고추원액을 제조하는 단계, 및 상기 키토산 희석액, 고추원액 및 정제수를 혼합하는 단계를 포함하는 것을 특징으로 하는 살균ㆍ살충제 조성물의 제조 방법을 제공한다.Another embodiment of the present invention provides a method for producing a sterilizing / insecticide composition, comprising the steps of preparing a chitosan diluent, preparing a red pepper stock solution, and mixing the chitosan diluent, red pepper stock solution and purified water. do.

상기 발명의 일 실시예는 키토산 희석액을 제조하는 단계가 키토산 원액을 1800 내지 2200 배 희석시키는 단계를 포함하는 것을 특징으로 한다.One embodiment of the invention is characterized in that the step of preparing a chitosan diluent comprises diluting the chitosan stock solution 1800 to 2200 times.

상기 발명의 일 실시예는 고추원액을 제조하는 단계가 붉은 고추 30개 내지 40개를 각각 세로로 이등분하여 씨를 채취하여 두고, 이등분된 고추를 다시 가로로 3~5등분하는 고추 가공단계, 알콜도수가 35°~40°인 주류 0.9ℓ내지 1.2ℓ, 채취된 고추, 가공된 고추씨, 및 정제수 1.8ℓ 내지 2.2ℓ를 혼합하는 혼합물 제조단계, 상기 혼합물을 밀봉하고 직사광선을 피하여 실온(20℃~25℃)에 1개월간 숙성시키는 숙성단계를 포함하는 것을 특징으로 한다.In one embodiment of the present invention, the step of preparing the red pepper stock solution is divided into 30 to 40 red pepper vertically, respectively, to collect seeds, pepper processing step of dividing the bisected pepper 3 to 5 horizontally again, alcohol also Mixture manufacturing step of mixing 0.9 l to 1.2 l of liquor having a number of 35 ° to 40 °, harvested red pepper, processed red pepper seed, and 1.8 l to 2.2 l of purified water, sealing the mixture and avoiding direct sunlight at room temperature (20 ° C. to 25 ° C.). ℃) is characterized in that it comprises a aging step of aging for 1 month.

상기 발명의 일 실시예는 키토산 희석액, 고추원액 및 정제수를 혼합하는 단계가 키토산 희석액 20~40 중량%, 고추원액 10~30중량%, 및 정제수 40~60 중량% 를 혼합하는 단계를 포함하는 것을 특징으로 한다.In one embodiment of the present invention, the step of mixing the chitosan diluent, the red pepper stock solution and purified water comprises 20 to 40% by weight of the chitosan diluent, 10 to 30% by weight red pepper stock solution, and 40 to 60% by weight purified water It features.

이하 실시예를 통하여 본 발명을 상세히 설명한다. 단, 본 발명이 하기 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail through examples. However, the present invention is not limited to the following examples.

실시예 1Example 1

고추원액의 제조Preparation of Red Pepper Stock Solution

고추는 매우 매운 것으로 사용했다. 이것은 살균력 있는 캡사이신의 함유량이 증가할수록 고추의 매운 맛이 강해지기 때문이다. 음성, 청양, 임실지방에서 재배된 작고 붉은 고추가 바람직하다. 길이가 8㎝내지 10㎝인 고추 30~40개를 준비하였다. 캡사이신은 고추씨에 많이 함유되어 있으므로, 고추씨를 채취하여 고추원액 제조에 이용하는 것이 바람직하다. 상기 고추 각각을 세로로 이등분하여 씨를 채취해두고, 이등분된 고추를 다시 가로로 3~5등분하였다. 그리고 알콜도수가 35°~40°인 소주를 준비하였다. 알콜도수가 높은 것이 고추씨 및 고추껍질의 캡사이신 성분을 추출ㆍ숙성시키는 능력이 우수하다. 또한 주류에 특별히 제한을 두는 것은 아니나 소주를 사용하는 것이 바람직하다. 상기 소주 1ℓ에 가공된 고추 및 고추씨를 넣었다. 소주의 양은 고추 30~40개를 사용할 경우 0.9~1.2ℓ의 양을 사용하는 것이 바람직하다. 0.9ℓ이하일 경우 캡사이신 성분을 충분히 추출ㆍ숙성시키지 못하고, 1.2ℓ이상일 경우 추출된 캡사이신 성분 함량이 낮아 살균ㆍ살충제 조성물의 효능이 낮아지는 문제가 있다. 그런 후 정제수를 2ℓ부어 혼합한 뒤 밀봉했다. 정제수의 양은 고추 30~40개, 소주 0.9~1.2ℓ일 때, 1.8~2.2ℓ의 양을 사용하는 것이 바람직하다. 이렇게 준비된 것을 직사광선을 피하여 실온(20℃~25℃)에 1개월간 두어 숙성시켰다. 특별히 온도에 제한을 두는 것은 아니나, 20℃이하의 온도에서는 캡사이신 성분의 추출ㆍ숙성이 충분히 일어나지 못하고, 25℃이상의 온도에서는 추출ㆍ숙성 속도가 빨라지나, 1개월 정도의 숙성기간을 예상할 때 고온에서의 숙성을 필요로 하는 것은 아니다. 또한 고온에서 단시간내에 숙성하는 것보다, 실온(20~25℃)에서 1개월 정도 숙성시킨 고추원액을 포함한 조성물의 살균ㆍ살충 효과가 우수함을 하기 실험으로 확인하였다.Red pepper was used as a very spicy one. This is because the higher the content of capsaicin, which is bactericidal, the stronger the pungent taste of red pepper. Small red peppers grown in Negative, Cheongyang and Imsil provinces are preferred. 30 to 40 peppers having a length of 8 cm to 10 cm were prepared. Since capsaicin is contained in a lot of red pepper seeds, it is preferable to collect red pepper seeds and use them for preparing red pepper stock solution. The pepper was divided into two vertically, and the seeds were collected, and the bisected pepper was further divided into three to five horizontally. And soju with alcohol degree 35 degrees-40 degrees was prepared. The high alcohol content is excellent in the ability to extract and ripen the capsaicin components of red pepper seeds and red pepper shells. Although alcohol is not particularly limited, it is preferable to use shochu spirits. 1 liter of the soju was added to the processed pepper and pepper seeds. The amount of shochu is preferably 0.9 ~ 1.2ℓ when using 30-40 peppers. If it is less than 0.9l, the capsaicin component is not sufficiently extracted and matured, if more than 1.2l there is a problem that the content of the extracted capsaicin component is low and the efficacy of the sterilizing and insecticide composition is lowered. Then, 2 liters of purified water was poured, mixed and sealed. When the amount of purified water is 30-40 peppers, shochu 0.9-1.2 L, it is preferable to use the amount of 1.8-2.2 L. Thus prepared material was aged for one month at room temperature (20 ° C-25 ° C), avoiding direct sunlight. Although there is no particular limitation on temperature, the extraction and maturation of capsaicin components does not occur sufficiently at temperatures below 20 ° C, and the extraction and maturation speeds up at temperatures above 25 ° C, but high temperatures are expected when the maturation period is about 1 month. It does not require aging in Esau. In addition, it was confirmed by the following experiment that the sterilization and insecticidal effect of the composition including the red pepper stock solution aged at room temperature (20-25 ° C.) for about one month rather than aging at high temperature in a short time.

실시예 2Example 2

고추원액의 숙성온도 및 기간에 따른 살균력 테스트Test of sterilization according to ripening temperature and duration of red pepper stock solution

실험 1Experiment 1

농업용으로 시판되는 키토산 원액을 준비하였다. 상기 키토산 원액을 1800배 내지 2200배 희석하는 것으로 키토산 희석액을 제조하였다. 그런후 키토산 희석액 30중량%, 실시예 1의 방법으로 제조된 고추원액 20중량%, 및 정제수 50중량%를 혼합하여 살균ㆍ살충제 조성물을 제조하였다. 제조된 살균ㆍ살충제 조성물을 역병이 발병한 고추 작물 20포기의 고추 열매, 잎, 줄기에 평당 1ℓ의 양으로 1회 살포하고 병의 진행을 관찰하였다.Commercial chitosan stock solution was prepared for agriculture. The chitosan dilution was prepared by diluting the chitosan stock solution by 1800 to 2200 times. Then, 30% by weight of the dilution of chitosan, 20% by weight of red pepper stock solution prepared by the method of Example 1, and 50% by weight of purified water were mixed to prepare a bactericidal / insecticide composition. The prepared bactericidal / insecticide composition was sprayed once in an amount of 1 liter per pyeong to red pepper fruits, leaves and stems of 20 red pepper crops with late blight, and the progress of the disease was observed.

실험 2Experiment 2

고추, 고추씨, 소주, 및 정제수의 혼합물을 30℃의 온도로 20일간 숙성시키는 것을 제외하고 실시예 1과 동일한 방법으로 제조된 고추원액 20중량%, 상기 실험 1과 동일한 방법으로 제조된 키토산 희석액 30중량%, 및 정제수 50중량%를 포함하는 살균ㆍ살충제 조성물로 실험 1과 동일한 실험을 하였다.20% by weight of red pepper stock solution prepared in the same manner as in Example 1, except that the mixture of red pepper, red pepper seeds, shochu, and purified water was stirred at a temperature of 30 ° C. for 20 days. The same experiment as in Experiment 1 was conducted with a bactericidal / insecticide composition containing 50% by weight and 50% by weight of purified water.

처리결과를 아래 표 1에 나타내었다.The treatment results are shown in Table 1 below.

구분division 처리결과Processing result 실험 1Experiment 1 살포 후 5시간 만에 병세 완화, 3일 후 20포기 완전 치유Alleviation of illness in 5 hours after spraying, full healing of 20 bags in 3 days 실험 2Experiment 2 살포 후 12시간 만에 병세 완화, 5일 후 10포기 병세 완전치유. 20일 후 15포기 완전치유. 5포기는 그후로도 완전 치유되지 않음.Alleviation of disease within 12 hours after spraying and complete healing of 10 bags of disease after 5 days. Complete heal of 15 waives after 20 days. Abandonment does not heal completely afterwards.

실시예 3Example 3

살균력 테스트Sterilization Test

상기 실시예 2의 실험 1과 동일한 방법으로 제조된 살균ㆍ살충제 조성물을 이용하여 작물의 여러가지 병원균에 대한 살균효과를 실험하였다. 실험 대상은 잔디갈색퍼짐병이 발생한 골프장 잔디, 탄저병이 발생한 고추작물, 역병이 발생한 오이로 하였다. 골프장 잔디에는 평당 1ℓ를 잔디 위에 직접 살포하였고, 오이 및 고추 작물에는 동일한 양을 열매, 잎, 줄기에 살포 혹은 토양에 직접 관주하였다. 처리 결과를 아래 표 2에 나타내었다.The bactericidal effect against various pathogens of the crop was tested using the bactericidal / insecticide composition prepared in the same manner as in Experiment 1 of Example 2. The subjects were golf grasses with grass brown spread disease, pepper crops with anthrax, and cucumbers with late blight. In the golf course grass, 1 liter per pyeong was sprayed directly onto the grass, and the cucumber and pepper crops were sprayed on the fruit, leaves, stems or irrigated directly on the soil. The treatment results are shown in Table 2 below.

시험작물명Name of test crop 시험장소Test place 처리결과Processing result 잔디grass 경기도 분당Bundang, Gyeonggi-do 살포 후 3일 후 병세 완전 치유됨Fully healed three days after spraying 고추pepper 강원도 영월군Yeongwol-gun, Gangwon-do 토양에 직접 관주 1회후 병세 악화중단, 5일 후 재관주로 3일만에 병세완전치유The disease worsened after one-time irrigation directly in the soil, and completely healed in three days with re-entry after five days 고추pepper 충북 청양군Cheongyang-gun, Chungbuk 1회살포 후 3일만에 잎 및 열매에 군데군데 발견되던 탄저병 병반무늬 사라짐.Discoloration of anthrax lesions found in leaves and berries after 3 days of spraying. 고추pepper 충북 청원군Cheongwon-gun, Chungbuk 탄저병으로 고사직전의 작물에 3일에 한번씩 2주간 살포. 완전 소생.Anthrax sprays crops just before the dead for two weeks every three days. Full resuscitation. 오이cucumber 충북 청원군Cheongwon-gun, Chungbuk 1회 살포로 잠시 발견되는 역병의 증세 사라짐. 살포후 수분 후 효과 육안 확인 가능.Disappear symptoms of late blight from a single spray. After spraying, you can check the effect after moisture. 오이cucumber 충북 청원군Cheongwon-gun, Chungbuk 1회 관주 후 일주일만에 소생.Revives in a week after one cross-check.

상기 표에서와 같이 본 발명의 살균ㆍ살충제 조성물이 특히 골프장 잔디와 고추작물의 병충해에 탁월한 살충 효과를 나타내는 것을 확인할 수 있다.As shown in the above table, it can be confirmed that the bactericidal / insecticide composition of the present invention exhibits an excellent insecticidal effect particularly on the pests of golf grass and pepper crops.

실시예 4Example 4

키토산 희석액 및 고추원액의 중량비에 따른 살균력 비교.Comparison of bactericidal power according to the weight ratio of chitosan diluent and red pepper stock solution.

실험 1Experiment 1

실시예 2의 실험 1과 동일한 방법으로 제조된 살균ㆍ살충제 조성물을 탄저병이 발생하여 열매와 잎에 겹무늬가 형성된 고추 작물 20포기에 1회 살포하였다. 살포방법은 고추의 열매와 잎, 줄기에 평당 1ℓ의 양으로 하였다. 살포 후 탄저병 병반진행도를 2~3일 간격으로 육안관찰하였고 결과를 표 3에 나타내었다.An bactericidal / insecticide composition prepared in the same manner as in Experiment 1 of Example 2 was sprayed once to 20 bags of pepper crops in which anthrax was generated and the leaves and leaves were formed on the leaves. The method of spraying was 1 liter per pyeong on the fruit, leaves and stems of red pepper. After spraying, the progress of anthrax disease was visually observed at intervals of 2 to 3 days, and the results are shown in Table 3.

실험 2Experiment 2

키토산 희석액 10중량%, 고추원액 40중량%, 정제수 50중량%로 하는 것을 제외하고 상기 실험 1과 같은 방법으로 살균ㆍ살충제 조성물을 제조하여 고추 작물에 살포하였다.A sterilization / insecticide composition was prepared in the same manner as in Experiment 1 except that 10 wt% of chitosan diluent, 40 wt% of red pepper stock solution, and 50 wt% of purified water were used to spray the red pepper crops.

실험 3Experiment 3

키토산 희석액 50중량%, 고추원액 10중량%, 정제수 40중량%로 하는 것을 제외하고 상기 실험 1과 같은 방법으로 살균ㆍ살충제 조성물을 제조하여 고추 작물에 살포하였다.A sterilization / insecticide composition was prepared in the same manner as in Experiment 1 except that 50% by weight of chitosan diluent, 10% by weight of red pepper stock solution and 40% by weight of purified water were sprayed onto pepper crops.

다음 표 3은 실험 1~3에서 탄저병이 발병한 작물의 포기 수를 나타낸 것이다.Table 3 below shows the number of abandoned abandonment of anthrax in Experiments 1-3.

(단위: 포기)(Unit: Abandonment) 경과일Elapsed date 살포일Spray 2일2 days 4일4 days 6일6 days 8일8th 10일10 days 실험 1Experiment 1 2020 55 1One -- -- -- 실험 2Experiment 2 2020 55 -- -- 1One 55 실험 3Experiment 3 2020 1515 1414 1212 1212 1111

상기 표로부터 고추원액의 함량이 높아지면 살균력이 증가하는 것을 확인할 수 있다. 고추원액이 40중량% 포함된 실험 2의 조성물에서는 살포후 수 시간 후에박멸 효과가 나타나는 것이 관찰되었고 3일 후 병반무늬가 완전히 사라졌다. 그러나 8일 경과 후 다시 병반무늬가 나타나는 것이 관찰되었다. 고추원액은 단시간내에 병원균을 박멸시키는 장점이 있지만 계속 침입하는 병원균에 대해 지속적인 효과는 주지 못함을 알 수 있다. 반면, 키토산의 함량이 보다 높은 실험 1의 조성물의 경우 4일 째 이후부터 병원균이 박멸되고 또다시 재발하지도 않는다. 실험 3의 조성물은 키토산의 함유량이 높아 식물 스스로 병원균에 대항할 수 있는 항균력이 증가되어 점차 효과가 나타나지만 박멸에 시간이 걸리고 그 사이 고사하는 작물도 있었다.It can be seen from the table that the sterilization power increases when the content of the red pepper stock solution is increased. In the composition of Experiment 2 containing 40% by weight of red pepper stock solution, the eradication effect was observed after several hours after spraying, and the lesion pattern disappeared after 3 days. However, after 8 days the lesion pattern appeared again. The red pepper stock has the advantage of eradicating pathogens in a short time, but it can be seen that it does not have a lasting effect on the invading pathogens. On the other hand, in the case of the composition of Experiment 1, which has a higher content of chitosan, pathogens are eradicated after 4 days and do not recur. The composition of Experiment 3 has a high content of chitosan, which increases the antibacterial activity against the pathogen itself, but gradually takes effect, but there are also crops that die in the meantime.

실시예 5Example 5

키토산 희석액 및 고추원액의 중량비에 따른 작물의 수확량 비교.Comparison of Crop Yields by Weight Ratio of Chitosan Diluent and Red Pepper Stock Solution.

실시예 4의 실험 1~3의 조성물과 동일한 살균ㆍ살충제 조성물을 각각 제조하였다. 충북 청원군의 농가에서 점무늬병이 발병한 고추 작물 20포기씩에 각 조성물을 고추의 열매와 잎, 줄기에 평당 1ℓ의 양으로 1 회 살포하였다. 그리고 고추 작물의 수확량을 비교하여 결과를 아래 표 4에 나타내었다. 이때의 수확량은 병해가 없는 건실한 열매만을 대상으로 한 것이다.The same bactericidal / pesticide compositions as those in Experiments 1 to 3 of Example 4 were prepared. Each composition was sprayed once per 20 liters of pepper crops with leaves and stems of peppers in Cheongwon-gun, Chungbuk, Korea. And compare the yield of pepper crops are shown in Table 4 below. Yield at this time is only for healthy fruit without disease.

구분division 고추 수확량(개)Pepper yield () 실험 1Experiment 1 500500 실험 2Experiment 2 120120 실험 3Experiment 3 350350

상기 표로부터 키토산의 함량이 높은 실험 3의 조성물을 살포한 경우가 키토산의 함량이 낮은 실험 2의 조성물의 경우보다 고추 수확량에 있어서 우수함을 확인할 수 있다. 그러나 최고의 수확을 거두기 위해서는 키토산 희석액과 고추원액의 중량비를 실험 1의 조성물에 근접하도록 하는 것이 바람직하다.It can be seen from the table that the case of spraying the composition of Experiment 3 having a high content of chitosan was superior in the yield of red pepper than the composition of Experiment 2 having a low content of chitosan. However, in order to obtain the best harvest, it is desirable to bring the weight ratio of the chitosan diluent and the red pepper stock close to the composition of Experiment 1.

실시예 6Example 6

방제력 테스트Response test

고추종자를 2월 초순 온실의 배지에 파종하고 5월 초순까지 재배하여 고추 종묘를 수확하였다. 재배된 종묘를 이식할 노지에 실시예 2의 실험 1과 동일한 방법으로 제조된 살균ㆍ살충제 조성물을 평당 3~7ℓ살포ㆍ관주한 다음 고추종묘 20포기를 이식하였다. 그런 후 별도의 농약이나 비료를 주지않고 재배하여 8월 초순에 고추 열매를 수확하였다. 대조구 실험을 위해 노지에 살균ㆍ살충제 조성물을 살포하지 않고, 상기 고추종묘 20포기를 이식하여 역시 별도의 비료나 농약의 처리 없이 재배하였다. 그리고 8월 초순에 고추 열매를 수확하였다. 결과를 표 5에 나타내었다. 이때의 수확량은 병해가 없는 건실한 열매만을 대상으로 한 것이다.Pepper seeds were sown in a medium of greenhouse in early February and cultivated until early May to harvest pepper seedlings. The sterilized and insecticidal composition prepared in the same manner as in Experiment 1 of Example 2 was sprayed and irrigated 3-7 L per pyeong, followed by 20 seedlings of red pepper seedlings. Then, peppers were harvested in early August by cultivating them without additional pesticides or fertilizers. For control experiments, 20 spores of the red pepper seedlings were transplanted without spraying the sterilizing / pesticide composition on the field, and were also grown without treatment of a separate fertilizer or pesticide. And in early August, red peppers were harvested. The results are shown in Table 5. Yield at this time is only for healthy fruit without disease.

구분division 고사한 작물(포기)Dead Crops (Abandonment) 수확량(개)Yield () 실험구Experiment 없음none 340340 대조구Control 1414 3030

실험구에서는 20포기 고추종자 모두 고사하지 않고 고추 열매를 수확할 수 있었다. 그러나 대조구에서는 14포기나 고사하였다. 또한 실험구의 수확량은 대조구의 그것에 비해 약 11배로 향상된 차이를 보여주었다. 이것은 본 발명 살균ㆍ살충제 조성물의 우수한 방제력을 보여주는 것이다. 즉, 종묘를 이식하기 전에 노지에 미리 살균ㆍ살충제 조성물을 살포하는 것으로 토양 속의 기생번식하고 있는 병원균의 포자나 균사를 살균 또는 비활성하므로써 고추 작물에 발생하는 역병, 탄저병 등을 근원적으로 방제하는 것이다.In the experimental zone, all of the 20 red pepper seeds were able to harvest red pepper fruits without killing. However, the control group died 14 batches. In addition, the yield of the experimental group was about 11 times improved compared to that of the control. This shows the excellent control of the present bactericidal / insecticide composition. In other words, by spraying the sterilization and insecticide composition on the field before transplanting the seedlings, the late blight, anthrax, etc. occurring in pepper crops are fundamentally controlled by sterilizing or inactivating spores and mycelia of parasitic pathogens in the soil.

실시예 7Example 7

살충력 테스트Insecticide test

바퀴벌레 10마리를 풀어둔 직경 15㎝의 실험접시를 준비하였다. 실시예 2의 실험 1과 동일한 방법으로 제조된 살균ㆍ살충제 조성물 0.1ℓ를 바퀴벌레에 1회 살포하였다. 비교 실험을 위하여 삼성제약의 상품 "에프킬러"로 동일한 실험을 하였다.An experimental dish of 15 cm in diameter in which 10 cockroaches were released was prepared. 0.1 l of the bactericidal / insecticide composition prepared in the same manner as in Experiment 1 of Example 2 was sprayed once on the cockroach. For the comparative experiments, the same experiment was conducted with Samsung Pharmaceutical's "F-killer".

아래 표 6은 바퀴벌레에 대한 살충력 실험 결과를 나타낸 것이다.Table 6 below shows the insecticidal test results for cockroaches.

구분division 처리결과Processing result 살균ㆍ살충제 조성물Sterilization / Pesticide Composition 살포 후 즉시 바퀴벌레의 활동성 둔화, 3분후 5마리 사망, 5분후 박멸Immediately after spraying, cockroach activity slowed down, 5 died after 3 minutes, eradicated after 5 minutes 에프킬러F killer 살포 후 1분 뒤 발퀴벌레 활동성 둔화, 3분후 3마리 사망, 5분후 5마리 사망. 10분후 박멸.After 1 minute of spraying, paw worm activity slowed down, 3 died after 3 minutes, and 5 died after 5 minutes. Eradicate after 10 minutes.

본 발명의 살균ㆍ살충제 조성물은 바퀴벌레에 대해서도 빠르고 강력한 살충효과를 나타내었다. 따라서 인체에 무해한 살충제로서의 역할을 할 수 있게 된다.The bactericidal / insecticide composition of the present invention exhibited a fast and powerful insecticidal effect against cockroaches. Therefore, it can act as a pesticide that is harmless to the human body.

이와같이 조성된 본 발명은 키토산 희석액에 고추원액을 혼합하여 조성시킨 것이기 때문에 작물의 각종 병원균 및 해충을 완벽하게 박멸하면서, 농작물에 미네랄과 비타민을 보충하는 장점이 있어서 건강한 농작물의 생산을 가능하게 하여 작물의 생산성과 품질향상을 기대할 수 있으며, 인체에 무해한 천연제재로써 농약 살포에 따른 농약 중독이나 토양오염이 전혀 없게 된다. 특히 본 발명은 잔디의 병충해에 탁월한 살충효과를 발휘하므로 각종 오염으로 문제가 되고 있는 골프장 잔디에 사용하여 환경친화적인방법으로 골프장 잔디를 관리하는 것을 가능하게 한다. 또한 바퀴벌레 등 일반해충에 대해서도 우수한 살충력을 발휘하므로 인체에 무해한 살충제로서 사용될 수 있다.Thus, the present invention has been prepared by mixing the red pepper stock solution in chitosan diluent, so as to completely eradicate various pathogens and pests of the crop, and to supplement the crops with minerals and vitamins, it is possible to produce healthy crops It can be expected to improve productivity and quality, and it is a natural product that is harmless to the human body, so there is no pesticide poisoning or soil pollution by spraying pesticides. In particular, the present invention exhibits excellent pesticidal effects on the grass pests, so that it is possible to manage the golf course turf in an environmentally friendly way by using it on a golf course turf which is a problem due to various pollution. In addition, it can be used as a pesticide harmless to the human body because it exhibits excellent insecticidal properties against general insects such as cockroaches.

Claims (8)

키토산 희석액, 고추원액 및 정제수를 포함하는 것을 특징으로 하는 살균ㆍ살충제 조성물.A bactericidal / insecticide composition comprising a chitosan diluent, a red pepper stock solution and purified water. 제 1 항에 있어서, 키토산 희석액 20~40 중량%, 고추원액 10~30중량%, 및 정제수 40~60 중량% 포함하는 것을 특징으로 하는 살균ㆍ살충제 조성물.The bactericidal / insecticide composition according to claim 1, comprising 20 to 40% by weight of chitosan diluent, 10 to 30% by weight of red pepper stock solution, and 40 to 60% by weight of purified water. 제 1 항에 있어서, 키토산 희석액이 키토산 원액을 1800배 내지 2200배 희석시킨 것임을 특징으로 하는 살균ㆍ살충제 조성물.The bactericidal / insecticide composition according to claim 1, wherein the chitosan dilution liquid is diluted 1800 times to 2200 times the chitosan stock solution. 제 1 항에 있어서, 고추원액이 붉은 고추, 알콜도수 35°내지 40°인 주류, 및 정제수를 포함하는 것을 특징으로 하는 살균ㆍ살충제 조성물.The bactericidal / insecticide composition according to claim 1, wherein the red pepper stock solution comprises red pepper, alcoholic beverage having an alcohol content of 35 ° to 40 °, and purified water. 살균ㆍ살충제 조성물의 제조 방법에 있어서,In the method for producing a sterilizing / insecticide composition, 키토산 희석액을 제조하는 단계;Preparing a chitosan diluent; 고추원액을 제조하는 단계; 및Preparing a red pepper stock solution; And 상기 키토산 희석액, 상기 고추원액 및 정제수를 혼합하는 단계를 포함하는 것을 특징으로 하는 살균ㆍ살충제 조성물 제조방법.A method for producing a bactericidal / insecticide composition comprising mixing the chitosan diluent, the red pepper stock solution and purified water. 제 5 항에 있어서, 키토산 희석액을 제조하는 단계가 키토산 원액을 1800배 내지 2200배 희석시키는 단계를 포함하는 것을 특징으로 하는 살균ㆍ살충제 조성물 제조방법.The method according to claim 5, wherein the preparing of the chitosan diluent comprises diluting the chitosan stock solution 1800 times to 2200 times. 제 5 항에 있어서, 고추원액을 제조하는 단계가The method of claim 5, wherein preparing the red pepper stock solution 붉은 고추 30개 내지 40개를 각각 세로로 이등분하여 씨를 채취하여 두고, 이등분된 고추를 다시 가로로 3~5등분하는 고추 가공단계;30 to 40 red peppers are divided into two vertically, and the seeds are collected, and the red pepper processing step of dividing the bisected pepper back into three to five horizontally; 알콜도수가 35°~40°인 주류 0.9ℓ내지 1.2ℓ, 가공된 고추, 채취된 고추씨, 및 정제수 1.8ℓ내지 2.2ℓ를 혼합하는 혼합물 제조단계; 및Mixture manufacturing step of mixing the alcohol content of 0.9L to 1.2L alcohol, processed red pepper, harvested red pepper seeds, and 1.8L to 2.2L purified water having an alcoholity of 35 ° to 40 °; And 상기 혼합물을 밀봉하고 직사광선을 피하여 실온(20℃~25℃)에 1개월 간 숙성시키는 숙성단계를 포함하는 것을 특징으로 하는 살균ㆍ살충제 조성물 제조방법.A method for producing a sterilizing / insecticide composition, comprising the step of sealing the mixture and aging for 1 month at room temperature (20 ° C.-25 ° C.), away from direct sunlight. 제 5 항에 있어서, 키토산 희석액, 고추원액 및 정제수를 혼합하는 단계가 키토산 희석액 20~40 중량%, 고추원액 10~30중량%, 및 정제수 40~60 중량% 혼합하는 단계를 포함하는 것을 특징으로 하는 살균ㆍ살충제 조성물 제조방법.6. The method of claim 5, wherein the mixing of the chitosan diluent, the red pepper stock solution and the purified water comprises mixing 20 to 40 wt% of the chitosan diluent, 10 to 30 wt% of the red pepper stock solution, and 40 to 60 wt% of the purified water. A method for producing a bactericidal / insecticide composition.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009063099A1 (en) 2007-11-13 2009-05-22 Biodurcal, S.L. Protective coating that can be applied as a phytosanitary protection means to protect against agricultural pests and diseases and method for applying same
CN102640765A (en) * 2012-04-18 2012-08-22 王振伟 Green and novel crop pest repellent
KR101524231B1 (en) * 2013-01-24 2015-05-29 이화목 Natural insecticide composition

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009063099A1 (en) 2007-11-13 2009-05-22 Biodurcal, S.L. Protective coating that can be applied as a phytosanitary protection means to protect against agricultural pests and diseases and method for applying same
CN102640765A (en) * 2012-04-18 2012-08-22 王振伟 Green and novel crop pest repellent
KR101524231B1 (en) * 2013-01-24 2015-05-29 이화목 Natural insecticide composition

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