KR20040033376A - Cosmetics composition comprising sophorolipids - Google Patents

Cosmetics composition comprising sophorolipids Download PDF

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KR20040033376A
KR20040033376A KR1020020062439A KR20020062439A KR20040033376A KR 20040033376 A KR20040033376 A KR 20040033376A KR 1020020062439 A KR1020020062439 A KR 1020020062439A KR 20020062439 A KR20020062439 A KR 20020062439A KR 20040033376 A KR20040033376 A KR 20040033376A
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composition
skin
acne
corynebacterium
examples
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KR1020020062439A
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Korean (ko)
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김정철
한상길
김윤석
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주식회사 엘지생활건강
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60—Sugars; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Cosmetics (AREA)

Abstract

PURPOSE: Provided is a cosmetics composition comprising, as an active ingredient, sophorolipids which are produced from Candida bombiocola(ATCC 22214). The cosmetic composition has excellent sterilization effect on Propionibacterium acne, Staphylococcus, Micrococcus, Corynebacterium, Propionibacterium and the like, as well as moisturization and softening effects on the skin. CONSTITUTION: A cosmetics composition is characterized by comprising, as a biosurfactant, 0.01-10 wt.% of sophorolipids which are produced from Candida bombiocola(ATCC 22214). The composition is formulated into face lotion, nutritive emulsion, face cream and the like.

Description

소포로리피드를 포함하는 화장료 조성물{COSMETICS COMPOSITION COMPRISING SOPHOROLIPIDS}Cosmetic composition containing phospholipids {COSMETICS COMPOSITION COMPRISING SOPHOROLIPIDS}

[산업상 이용 분야][Industrial use]

본 발명은 소포로리피드를 포함하는 화장료 조성물에 관한 것으로, 더욱 상세하게는 미생물로부터 생산된 생물계면활성제인 소포로리피드를 유효성분으로 포함하는 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition comprising vesicle lipids, and more particularly, to a cosmetic composition comprising vesicle lipids, which are biosurfactants produced from microorganisms, as an active ingredient.

[종래 기술][Prior art]

생물계면활성제란 생물 유래의 계면활성물질을 모두 포괄하는 용어이지만, 흔히 미생물에 의해 생산되는 계면활성제를 말하는 것이다. 합성계면활성제의 피부 및 인체 안정성과 생분해 등의 문제와 이의 사용량 증가로 인한 여러 가지 환경 오염 문제가 점차 확대되면서 생물계면활성제의 연구 및 응용가능성이 증대되고 있는 실정이다. 이는 생물계면활성제가 종래의 합성 계면활성제와 달리 높은 생분해성과 안정성을 가지고 있고, 또한 합성 계면활성제에서는 볼 수 없는 구조상의 특징으로 인해 새로운 계면화학적 기능과 성능을 나타낼 수 있기 때문이다.Biosurfactant is a term encompassing all surfactants derived from living organisms, but often refers to surfactants produced by microorganisms. As the problems such as skin and human stability and biodegradation of synthetic surfactants and various environmental pollution problems caused by the increased use thereof, research and application of biosurfactants are increasing. This is because biosurfactants have high biodegradability and stability, unlike conventional synthetic surfactants, and may exhibit new surface chemical function and performance due to structural features not found in synthetic surfactants.

현재 생물계면활성제를 실용화한 예로, 일본의 가오화장품 회사(KAO Corp.)의 경우 토룰롭시드속(Torulopsis sp.) 효모로부터 당지질계 계면활성제를 생산하여 생물유화제(Bioemulsifier) 및 피부보습제로서 화장품에 실용화하고 있고, 바실러스 폴리믹사(Bacillus polymyxa)라는 박테리아에 의해 생산되는 미생물 고분자(Biopolymer)도 화장품 및 샴푸의 유용성분으로 이용되고 있다고 보고되어 있다.As an example of the biosurfactant in practical use, Japanese Kao Corp. (KAO Corp.) produces glycolipid surfactants from Torulopsis sp. Yeast to produce cosmetics as bioemulsifiers and skin moisturizers. In practical use, it is reported that a microbial polymer produced by a bacterium called Bacillus polymyxa is also used as a useful ingredient in cosmetics and shampoos.

한편, 여드름이란 면포(comedone), 구진(papule), 농포(pustule), 낭종, 또는 결절 형성을 특징으로 하는 모낭피지선의 만성 염증성 질환을 일컫는다. 여드름의 발병원인은 아직도 명확히 규명되어 있지 못하며, 다만 유전적 요인과 환경적 요인을 배제한다면 다음의 세 가지의 중요한 원인이 여드름 발병에 직접적으로 관여한다는 것이 지금까지의 연구 보고를 통하여 정설로서 인정되고 있다. 첫째는 과잉으로 분비되는 피지로, 피지의 형성은 남성호르몬의 작용에 의하여 이루어지며, 이 남성 호르몬의 비정상적 증가 또는 이와 관련된 여러 기전을 통하여 피지선은 기능이 항진되고 그 결과 과잉의 피지를 생성하게 된다. 둘째는 모낭의 과각화로 인한 모공의 폐쇄를 들 수 있다. 생성된 피지는 피부 밖으로 원활하게 배출되어야 하지만, 모낭의 과각화로 인하여 모공이 막혀 피지의 원활한 배출이 억제되고 그 결과 모낭내에 피지가 정체되어 미세면포를 형성하게 된다. 세 번째는 여드름균(Propionibacterium acnes)의 존재 때문으로 이것은 여드름 병변 악화의 중요한 원인으로 설명된다. 피지선내에 여드름 균의 증식이 많아짐에 따라 여드름 균에 의해 피지를 구성하고 있는 트리글리세라이드(triglyceride)가 자유 지방산(free fatty acid)으로 전환되어서 자극원으로 작용하거나, 면포유발성(comedogenic) 물질로 작용하여 여드름 생성을 촉진시켜 준다. 또한 더욱 진행되어 염증반응까지 수반하게 된다. 따라서, 현재까지 여드름 완화를 목적으로 하는 평가도 상기와 같은 작용 기전을 이용하여 피지분비 조절, 과각화 억제, 및 여드름 균에 대한 살균력 등을 평가하는 방법을 이용하여 수행되고 있다.On the other hand, acne refers to chronic inflammatory diseases of the hair follicle sebaceous glands characterized by the formation of comedones, papules, pustules, cysts, or nodules. The causes of acne are still not clearly identified, except that genetic and environmental factors may be directly involved in acne outbreaks. have. The first is sebum that is excessively secreted. The formation of sebum is caused by the action of male hormones, and the abnormal increase of this testosterone or various mechanisms associated with it causes sebaceous glands to become hyperfunctional, resulting in excess sebum. . Second is the closure of pores due to hyperkeratosis. The produced sebum should be discharged smoothly out of the skin, but the pores are blocked due to the exaggeration of the hair follicles, thereby suppressing the smooth discharge of sebum, and as a result, sebum is stagnated in the hair follicles to form microsurfaces. Third, due to the presence of propionibacterium acnes , this is explained as an important cause of acne lesion deterioration. As acne bacteria proliferate in the sebaceous glands, triglycerides that make up sebum are converted into free fatty acids and act as irritants or acne-producing substances. To promote acne production. It also progresses further and involves inflammatory reactions. Therefore, until now, evaluation for the purpose of alleviating acne has been carried out using a method of evaluating sebum secretion control, hyperkeratosis, and bactericidal activity against acne bacteria using the above-described mechanism of action.

이러한 여드름을 치료하기 위하여 종래에는 살리실산이나 비타민 A 유도체인 레티노익산 제제를 사용하여 모공의 각질 제거를 통하여 피지 분비를 원활하게 함으로써 치료효과를 가지게 하려는 시도가 있었으며, 또는 벤조일 퍼옥사이드 제제를 사용하여 피부 각질 제거와 강력한 항균작용을 통해서 치료를 하고자 하는 시도가 있었다. 그러나, 상기 살리실산 제제의 경우 미비한 치료 효과 뿐만 아니라 피부 발적, 부종 또는 피부 기피증 등을 일으킬 수 있으며, 레티노익산 제제의 경우 과각화 억제를 통한 효과는 있으나 접촉성 피부염, 홍반, 피부 건조, 및 박리현상 등이 일어날 수 있고, 또한 벤조일 퍼옥사이드 제제의 경우 알레르기성 접촉 피부염 및 상처를 남기고 심한 홍반이 생기는 등의 여러 부작용이 보고되고 있다. 또한 비교적 최근에 소개된 아젤래익 에시드(Azelaci acid) 제제의 경우 약물 부작용은 감소되었지만 기존 치료제 제품에 비하여 탁월한 임상 효과는 보고되지 못하는 실정이다.In order to treat such acne in the past, attempts have been made to have a therapeutic effect by smoothing the secretion of sebum through exfoliation of pores using salicylic acid or vitamin A derivative retinoic acid preparations, or by using benzoyl peroxide preparations. Attempts have been made to cure through exfoliation and strong antimicrobial activity. However, the salicylic acid preparations may cause skin redness, swelling or skin respiratory disease as well as insignificant therapeutic effects. In the case of retinoic acid preparations, the effect of hyperkeratosis is suppressed, but contact dermatitis, erythema, dry skin, and exfoliation may occur. This can happen, and many side effects have also been reported for benzoyl peroxide preparations such as allergic contact dermatitis and scarring and severe erythema. In addition, the relatively recently introduced azelaci acid preparations have reduced drug side effects, but excellent clinical effects are not reported compared to conventional therapeutic products.

또한, 인체의 체취는 인체에서 분비된 에크린땀(eccrine sweat), 아포크린땀(apocrine sweat), 피지, 오염물을 미생물 등이 분해시킴으로서 발생하게 된다. 즉, 처음 분비된 에크린땀이나 아포크린땀은 무취이나 피부표피, 털, 땀구멍 등에 상재하는 여러 미생물, 특히 체취와 관련된 미생물들의 작용으로 불쾌감을 일으키는 액취, 족취, 두취 등의 체취를 유발하게 된다. 피부에는 수많은 상재균이 존재하는데, 대표적인 것으로는 포도상구균인 스타필로코커스(Staphylococcus), 구균인 마이크로코커스(Micrococcus), 코리네박테리움(Corynebacterium), 프로피오니박테리움(Propionibacterium) 등이 있으며, 특히 코리네박테리움이 코를 자극하는 불쾌취로 강한 냄새 소유자에서 많이 발견되는 것으로 알려져 있다. 또한, 체취는 미생물의 분포수와도 밀접한 관계가 있는데, 겨드랑이, 발, 머리의 경우 기타 부위보다 미생물이 많이 존재하며, 이는 미생물이 살 수 있는 환경, 즉 털의 유무, 적정온도, 습도 등이 조성되기 때문인 것으로 밝혀져 있다.In addition, the body odor is generated by microorganisms decomposing eccrine sweat (apoccrine sweat), apocrine sweat (apocrine sweat), sebum, contaminants secreted by the human body. That is, the first secreted eccrine sweat or apocrine sweat causes odors such as odors, foot odors, and head odors caused by the action of various microorganisms, especially those associated with body odor, which are odorless or skin epidermis, hair, and pores. There are numerous floras on the skin, including Staphylococcus , Staphylococcus, Micrococcus , Corynebacterium , and Propionibacterium . Corynebacterium is known to be found in many strong odor holders due to nasal irritability. In addition, body odor is closely related to the distribution of microorganisms, and in the case of armpits, feet, and hair, there are more microorganisms than other parts. It turns out that it is because it is formed.

이러한 인체 체취에 대처할 수 있는 방법 중 항균 작용에 의한 체취 억제 방법으로 여러 연구가 진행되었으며, 항균 물질로는 트리클로산, 트리클로카반 등의 합성 물질이 알려져 있다. 그러나, 이러한 합성 항균 물질은 항균 작용은 우수하나, 피부 부작용 등의 문제가 있는 것으로 알려져 있다.Various studies have been conducted as a method for suppressing the body odor by the antibacterial action among the methods that can cope with the human body odor, and synthetic materials such as triclosan and triclocarban are known as antibacterial substances. However, these synthetic antimicrobial agents are known to have excellent antibacterial effects, but have problems such as skin side effects.

본 발명은 상기와 같은 종래 기술의 문제점을 해결하기 위하여, 여드름 균에 대한 항균 효과가 우수한 화장료 조성물을 제공하는 것을 목적으로 한다.In order to solve the problems of the prior art as described above, an object of the present invention is to provide a cosmetic composition excellent in antibacterial effect against acne bacteria.

본 발명의 다른 목적은 액취균에 대한 항균 효과가 우수한 화장료 조성물을 제공하는 것이다.Another object of the present invention is to provide a cosmetic composition excellent in antimicrobial effect against liquid odor bacteria.

본 발명의 다른 목적은 피부 보습효과, 사용감촉 및 피부유연 효과가 우수한 화장료 조성물을 제공하는 것이다.Another object of the present invention is to provide a cosmetic composition excellent in skin moisturizing effect, feeling of use and skin softening effect.

상기 목적을 달성하기 위하여, 본 발명은 캔디다 봄비오콜라(Candida bombiocola)(ATCC 22214)로부터 생산된 소포로리피드를 유효성분으로 포함하는 화장료 조성물을 제공한다.In order to achieve the above object, the present invention provides a cosmetic composition comprising a sopolori lipid produced from Candida bombiocola (ATCC 22214) as an active ingredient.

이하에서 본 발명을 상세하게 설명한다.Hereinafter, the present invention will be described in detail.

본 발명자는 종래 기술의 문제점을 해결하기 위하여, 화장료 조성물의 유효성분으로 합성화학물질이 아닌 천연물인 캔디다 봄비오콜라로부터 생산된 생물계면활성제인 소포로리피드를 이용할 경우 통상의 처방 제품에 비해 피부보습 및 유연효과가 우수하고, 여드름 균에 대한 항균 효과 및 액취균에 대한 항균 효과도 매우 우수함을 확인하여 본 발명을 완성하게 되었다.In order to solve the problems of the prior art, the present inventors use skin bio-surfactant, a biosurfactant produced from Candida Bombbio Cola, which is not a synthetic chemical, as an active ingredient of a cosmetic composition. And excellent in the softening effect, confirming the antibacterial effect against acne bacteria and antibacterial effect against liquid odor bacteria was completed the present invention.

본 발명에서 사용되는 생물계면활성제 생산 미생물은 효모의 일종인 캔디다 봄비오콜라(Candida bombiocola)를 사용한다. 상기 캔디다 봄비오콜라가 당지질계 계면활성제인 소포로리피드를 생산한다는 사실은 알려져 있으나, 다른 효과에 대해서는 아직까지 미미한 실정이다. 따라서, 본 발명에서는 소포로리피드의 계면활성제 역할 이외에, 피부보습효과 뿐만 아니라 여드름 및 액취균에 대한 살균효과 등을 새로이 확인하였다.The biosurfactant producing microorganism used in the present invention uses Candida bombiocola , a kind of yeast. It is known that the candida bombio cola produces a lipophilic surfactant, a glycolipid-based surfactant, but other effects are still insignificant. Therefore, in the present invention, in addition to the role of the surfactant of the vesicle lipids, as well as the skin moisturizing effect and the bactericidal effect against acne and odor bacteria newly confirmed.

본 발명의 화장료 조성물에서 유효성분으로 사용되는 소포로리피드는 계대 배양된 종균을 고압 멸균된 발효기에 접종하고 배양한 후, 원심분리 및 추출을 통해 얻을 수 있다.Sophorolipide used as an active ingredient in the cosmetic composition of the present invention can be obtained by inoculating and incubating passaged spawn seed in a high-pressure sterilized fermentor, followed by centrifugation and extraction.

이러한 소포로리피드는 하기 화학식 1 또는 화학식 2로 표시되는 구조를 갖는 것이 바람직하다.Such phospholipids preferably have a structure represented by the following general formula (1) or (2).

[화학식 1][Formula 1]

[화학식 2][Formula 2]

상기 식에서, R1, R2, 및 R3는 각각 독립적으로 또는 동시에 수소, 또는 -COCH3이며, R4는 탄소수 1 내지 20의 직쇄 또는 가지달린 알킬기이다.Wherein R 1 , R 2 , and R 3 are each independently or simultaneously hydrogen, or —COCH 3 , and R 4 is a straight or branched alkyl group having 1 to 20 carbon atoms.

본 발명에서 유효성분으로 포함하는 소포로리티드의 함량은 0.01 내지 10 중량%로 사용하는 것이 바람직하다. 상기 소포로리피드의 함량이 0.01 중량% 미만이면 소포로리피드의 의한 효과인 피부보습효과, 사용감촉과 피부유연효과, 및 여드름균과 액취균에 대한 항균효과가 거의 나타나지 않으며, 10 중량%를 초과하면 상승효과가 없고 사용감과 피부 촉감을 떨어뜨리는 경향이 있다.In the present invention, it is preferable to use the content of the sorocoretides as an active ingredient in an amount of 0.01 to 10% by weight. When the content of the vesicle lipid is less than 0.01% by weight, the skin moisturizing effect, the feeling of use and the skin softening effect, the antibacterial effect of the acne bacteria and the liquid odor bacteria, which are the effect of the sorborelipid, are rarely exceeded and exceeded 10% by weight. This has no synergistic effect and tends to degrade the feel and feel of the skin.

본 발명의 소포로리피드를 유효성분으로 포함하는 화장료 조성물은 일반 피부화장료에 배합되는 임의의 성분들을 필요에 따라 적절히 배합하여 사용할 수 있다. 상기 임의의 성분으로는 유분, 정제수, 계면활성제, 보습제, 저급알코올, 증점제, 킬레이트제, 색소, 방부제, 자외선 차단제, 산화방지제, 염료, 향료 등이 있다. 본 발명의 화장료 조성물의 예를 들면 화장수, 영양유액, 크림 또는 팩의 형태로 제형화되는 것이 바람직하지만, 이에 한정되는 것은 아니며, 여러 가지 다른 제형으로도 응용이 가능하다.The cosmetic composition comprising the vesicle lipid of the present invention as an active ingredient may be used by appropriately blending any ingredients blended into general skin cosmetics as needed. The optional components include oils, purified water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments, preservatives, sunscreens, antioxidants, dyes, fragrances and the like. For example, the cosmetic composition of the present invention is preferably formulated in the form of a lotion, nutrient, cream or pack, but is not limited thereto, and may be applied to various other formulations.

이하 본 발명을 하기 실시예 및 비교예를 참조로 하여 설명한다. 그러나, 이들 예는 본 발명을 예시하기 위한 것일 뿐, 본 발명이 이들에 한정되는 것은 아니다.Hereinafter, the present invention will be described with reference to the following Examples and Comparative Examples. However, these examples are only for illustrating the present invention, and the present invention is not limited thereto.

[실시예]EXAMPLE

제조예 1Preparation Example 1

(소포로리피드의 생산)(Production of phospholipid)

균주는 캔디다 봄비오콜라(Candida bombiocola) ATCC 22214를 사용하였으며, 사용한 배지의 조성은 다음과 같다. Candida bombiocola ( Candyda bombiocola ) ATCC 22214 was used, the composition of the medium was as follows.

배지 조성: 효모 추출물 3.0 g, 비프 익스트랙트 10.0 g, 펩톤 10.0 g, 가용성 전분 1.0 g, 포도당 5.0 g, L-시스테인 하이드로클로라이드 0.5 g, 염화나트륨 5.0 g, 소듐아세테이트 3.0 g, 아가 0.75 g, pH 6.8Medium composition: 3.0 g yeast extract, 10.0 g beef extract, 10.0 g peptone, 1.0 g soluble starch, 5.0 g glucose, 0.5 g L-cysteine hydrochloride, 5.0 g sodium chloride, 3.0 g sodium acetate, 0.75 g agar, pH 6.8

48 시간 동안 100 ml 플라스크에서 계대 배양한 종균을 고압멸균한 2.5 L 발효기에 접종하여 7일 동안 배양하였다. 배양이 끝난 후, 소포로리피드를 원심분리와 에틸아세테이트를 이용한 추출로 분리하였다.The seedlings passaged in a 100 ml flask for 48 hours were inoculated in a autoclaved 2.5 L fermenter and incubated for 7 days. After the incubation, the phospholipids were separated by centrifugation and extraction with ethyl acetate.

(여드름 균에 대한 항균력 평가 실험)(Antibacterial activity evaluation experiment on acne bacteria)

상기 방법으로 얻어진 소포로리피드의 여드름균에 대한 항균력 실험은 10 ml의 비에취아이(BHI) 액체 배지에서 전 배양시킨 피.아크네스(Propionibacterium acnes)를 108cell/ml 농도가 되도록 접종하고 일정 농도의 소포로리피드를 가하여 혐기성 배양기에서 48 시간 동안 배양한 후 피.아크네스의 수를 측정하여 최소 억제 농도를 구하였다. 그 결과, 소포로리피드의 피.아크네스에 대한 최소 억제 농도 범위는 150 내지 200 ppm으로 평가되었다.Antimicrobial activity test against acne bacteria of Sopholori lipid obtained by the above method was inoculated to 10 8 cell / ml concentration of Propionibacterium acnes pre-incubated in 10 ml BHI liquid medium. After culturing for 48 hours in an anaerobic incubator with a concentration of phophorolipid, the minimum inhibitory concentration was determined by measuring the number of P. acnes. As a result, the minimum inhibitory concentration range for Sophylori lipids against P. acnes was evaluated to be 150 to 200 ppm.

(액취 균에 대한 항균력 평가 실험)(Antibacterial activity evaluation experiment against liquid odor bacteria)

액취균으로는 코리네박테리움 제로시스(Corynebacterium xerosis) ATCC 7711을 사용하였다. 소포로리피드의 코리네박테리움 제로시스에 대한 항균력 실험은 10 ml의 비에취아이(BHI) 액체 배지에서 전 배양시킨 뒤, 코리네박테리움 제로시스를 106cell/ml 농도가 되도록 접종하고 일정 농도의 소포로리피드를 가하여 호기성 배양기에서 48 시간 동안 배양한 후 코리네박테리움 제로시스의 수를 측정하여 최소 억제 농도를 구하였다. 그 결과, 소포로리피드의 코리네박테리움 제로시스에 대한 최소 억제 농도 범위는 500 ppm으로 평가되었다.Corynebacterium xerosis ATCC 7711 was used as the liquid odor bacteria. Antimicrobial activity of Corynebacterium zerosis of Sophorolipid was pre-incubated in 10 ml of BHI liquid medium, followed by inoculation of Corynebacterium zerosis to a concentration of 10 6 cells / ml After culturing for 48 hours in an aerobic incubator with a concentration of Sophorolipid, the minimum inhibitory concentration was determined by measuring the number of Corynebacterium zeroes. As a result, the minimum inhibitory concentration range of Sophorolipid against Corynebacterium zerosis was evaluated at 500 ppm.

실시예 1 내지 2 및 비교예 1Examples 1-2 and Comparative Example 1

하기 표 1과 같은 통상적인 화장수의 조성 및 제조예 1에서 제조한 생물계면활성제인 소포로리피드를 첨가한 조성으로 화장수를 제조하였다. 정제수에 글리세린, 프로필렌글리콜, 자외선차단제 및 소포로리피드를 가하여 실온에서 용해시켰다. 한편, 에탄올에 올레일알코올, 폴리옥시에틸렌솔비탄모노라우린산에스테르, 폴리옥시에틸렌모노라우릴에테르, 방부제 및 향료를 가하여 실온에서 용해시키고, 이를 전술한 정제수부에 가하여 가용화시켰다. 이후, 염료로 조색한 후 여과하여 제품화하였다.Toner was prepared in the composition of the conventional lotion as shown in Table 1 and the composition to which the biosurfactant phophorolipid prepared in Preparation Example 1 was added. Glycerin, propylene glycol, a sunscreen and phospholipid were added to the purified water and dissolved at room temperature. On the other hand, oleyl alcohol, polyoxyethylene sorbitan monolauric acid ester, polyoxyethylene monolauryl ether, a preservative, and a fragrance | flavor were added to ethanol, and it melt | dissolved at room temperature, and it was solubilized by adding to the above-mentioned purified water. Thereafter, the mixture was colorized and filtered to produce a product.

화장수의 조성(단위: 중량%)Composition of lotion (unit: weight%) 원료명Raw material name 실시예 1Example 1 실시예 2Example 2 비교예 1Comparative Example 1 글리세린glycerin 5.05.0 5.05.0 5.05.0 프로필렌글리콜Propylene glycol 4.04.0 4.04.0 4.04.0 올레일알코올Oleyl alcohol 0.10.1 0.10.1 0.10.1 폴리옥시에틸렌솔비탄모노라우린산에스테르Polyoxyethylene sorbitan monolauric acid ester 0.50.5 -- 1.51.5 폴리옥시에틸렌모노라우릴에테르Polyoxyethylene Monolauryl Ether 0.50.5 0.50.5 0.50.5 소포로리피드Sopolori lipid 0.20.2 0.50.5 -- 에탄올ethanol 10.010.0 10.010.0 10.010.0 향료Spices 0.10.1 0.10.1 0.10.1 염료dyes 적량Quantity 적량Quantity 적량Quantity 방부제, 자외선차단제Preservative, sunscreen 적량Quantity 적량Quantity 적량Quantity 정제수Purified water 100 중량% 까지Up to 100% by weight 100 중량% 까지Up to 100% by weight 100 중량% 까지Up to 100% by weight

실시예 3 내지 4 및 비교예 2Examples 3 to 4 and Comparative Example 2

하기 표 2와 같은 통상적인 영양유액 조성 및 제조예 1에서 제조한 생물계면활성제인 소포로리피드를 첨가한 조성으로 영양유액을 제조하였다. 정제수에 프로필렌글리콜과 소포로리피드를 가하여 가열 혼합한 다음, 에탄올을 넣어 70 ℃로 승온시켰다. 미리 조제한 카르복시비닐폴리머 수용액과 알칼리를 나머지 다른 친유성 성분과 혼합 가열하여 70 ℃가 되도록 하였다. 이후, 유상 부분을 가열하여 균일하게 혼합한 후, 알칼리를 가해서 중화시키고 호모게나이저(homogenizer)에 의해 균일하게 유화시킨 다음 열교환기에 의해 30 ℃까지 냉각시켜 제조하였다.A nutritive emulsion was prepared by adding a conventional nutritional fluid composition as shown in Table 2 below, and adding phospholipid, which is a biosurfactant prepared in Preparation Example 1. Propylene glycol and phospholipid were added to purified water, and the mixture was heated and mixed. Then, ethanol was added thereto and the temperature was raised to 70 ° C. The previously prepared aqueous solution of carboxyvinyl polymer and alkali were mixed and heated with the other lipophilic components to 70 ° C. Thereafter, the oil phase part was heated and uniformly mixed, neutralized by addition of alkali, uniformly emulsified by a homogenizer, and cooled to 30 ° C. by a heat exchanger.

영양유액의 조성(단위: 중량%)Composition of nutritional milk (unit: weight%) 원료명Raw material name 실시예 3Example 3 실시예 4Example 4 비교예 2Comparative Example 2 스쿠알렌Squalene 5.05.0 5.05.0 5.05.0 와셀린Waselin 2.02.0 2.02.0 2.02.0 밀납Beeswax 0.50.5 0.50.5 0.50.5 솔비탄세스퀴올레인산에스테르Solbitan sesquioleic acid ester 0.80.8 0.80.8 0.80.8 폴리옥시에틸렌올레일에테르Polyoxyethylene oleyl ether 0.50.5 -- 1.21.2 소포로리피드Sopolori lipid 0.20.2 2.02.0 -- 향료Spices 적량Quantity 적량Quantity 적량Quantity 산화방지제Antioxidant 적량Quantity 적량Quantity 적량Quantity 프로필렌글리콜Propylene glycol 5.05.0 5.05.0 5.05.0 에탄올ethanol 5.05.0 5.05.0 5.05.0 카르복시비닐폴리머Carboxy Vinyl Polymer 20.020.0 20.020.0 20.020.0 수산화칼륨Potassium hydroxide 0.10.1 0.10.1 0.10.1 정제수Purified water 100 중량% 까지Up to 100% by weight 100 중량% 까지Up to 100% by weight 100 중량% 까지Up to 100% by weight

실시예 5 내지 6 및 비교예 3Examples 5-6 and Comparative Example 3

하기 표 3과 같은 통상적인 크림의 조성 및 제조예 1에서 제조한 생물계면활성제인 소포로리피드를 첨가한 조성으로 크림을 제조하였다. 정제수에 프로필렌글리콜과 소포로리피드를 가하여 혼합한 다음, 70 ℃로 승온시켰다. 나머지 성분들은 혼합하여 가열 용해시켜 70 ℃가 되도록 하고 이를 수상부에 가해서 예비 유화시키고 호모게나이저(homogenizer)로 균일하게 유화시킨 다음 열교환기에 의해 실온까지 냉각시켜 제조하였다.To prepare a cream with a composition of a conventional cream as shown in Table 3 and the composition added to the biosurfactant phophorolipid prepared in Preparation Example 1. Propylene glycol and phospholipid were added to the purified water, mixed, and then heated to 70 ° C. The remaining ingredients were mixed and dissolved by heating to 70 ° C., preliminarily emulsified in the aqueous phase, homogenized with a homogenizer and cooled to room temperature by a heat exchanger.

크림의 조성(단위: 중량%)Composition of Cream (Unit: Weight%) 원료명Raw material name 실시예 5Example 5 실시예 6Example 6 비교예 3Comparative Example 3 스테아린산Stearic acid 2.02.0 2.02.0 2.02.0 스테아릴알코올Stearyl alcohol 7.07.0 7.07.0 7.07.0 환원라놀린Reduced lanolin 2.02.0 2.02.0 2.02.0 스쿠알렌Squalene 5.05.0 5.05.0 5.05.0 옥틸도데카놀Octyldodecanol 6.06.0 6.06.0 6.06.0 폴리옥시에틸렌세틸에테르Polyoxyethylene cetyl ether -- 1.01.0 3.03.0 친유형모노스테아린산글리세린Lipophilic Monostearic Acid Glycerin 2.02.0 2.02.0 2.02.0 소포로리피드Sopolori lipid 2.02.0 0.20.2 -- 향료Spices 적량Quantity 적량Quantity 적량Quantity 방부제, 산화방지제Preservatives, Antioxidants 적량Quantity 적량Quantity 적량Quantity 프로필렌글리콜Propylene glycol 5.05.0 5.05.0 5.05.0 정제수Purified water 100 중량% 까지Up to 100% by weight 100 중량% 까지Up to 100% by weight 100 중량% 까지Up to 100% by weight

실시예 7 내지 8 및 비교예 4Examples 7 to 8 and Comparative Example 4

하기 표 4와 같은 통상적인 팩의 조성 및 제조예 1에서 제조한 생물계면활성제인 소포로리피드를 첨가한 조성으로 팩을 제조하였다. 정제수에 솔비트와 소포로리피드를 가하여 혼합하고 여기에 산화티탄과 카올린, 산화비닐수지에멀션을 첨가한 다음, 일부의 에탄올로 습윤시킨 폴리비닐알코올을 가하여 70 ℃로 가열 용해시켰다. 남은 알코올에 향료, 방부제를 가하여 혼합하고 최종적으로 올리브유를 첨가하여 냉각시켜 제품을 만들었다.To prepare a pack with the composition of the conventional pack as shown in Table 4 and the composition added to the biosurfactant phophorolipid prepared in Preparation Example 1. Solbite and sorbolipid were added to the purified water, mixed with titanium oxide, kaolin and an emulsion of vinyl oxide resin, and polyvinyl alcohol moistened with some ethanol was added thereto, followed by heating and dissolving at 70 ° C. Flavor and preservatives were added to the remaining alcohol and mixed, and finally, olive oil was added to cool the product.

팩의 조성(단위: 중량%)Composition of the pack (unit: wt%) 원료명Raw material name 실시예 7Example 7 실시예 8Example 8 비교예 4Comparative Example 4 초산비닐수지에멀션Vinyl acetate resin emulsion 15.015.0 15.015.0 15.015.0 폴리비닐알코올Polyvinyl alcohol 10.010.0 10.010.0 10.010.0 올리브유olive oil 3.03.0 3.03.0 3.03.0 솔비트Solbit 3.03.0 0.50.5 5.05.0 소포로리피드Sopolori lipid 0.20.2 2.02.0 -- 산화티탄Titanium oxide 8.08.0 8.08.0 8.08.0 카올린kaoline 7.07.0 7.07.0 7.07.0 에탄올ethanol 5.05.0 5.05.0 5.05.0 향료Spices 적량Quantity 적량Quantity 적량Quantity 방부제antiseptic 적량Quantity 적량Quantity 적량Quantity 정제수Purified water 100 중량% 까지Up to 100% by weight 100 중량% 까지Up to 100% by weight 100 중량% 까지Up to 100% by weight

실험예 1Experimental Example 1

(수분보유능 측정시험)(Moisture retention test)

소포로리피드의 수분 보유능을 알아보기 위하여, 손등 부위가 건조한 성인 남ㆍ여 20 명을 대상으로 1일 2회씩 총 3개월 동안 다음과 같은 실험을 수행하였다. 먼저 피시험자 손등의 4cm × 4cm 면적 부분에 각각 사용 전후의(1개월) 피부의 수분함량을 피부수분함량 측정장치(스킨 설피스 하이그로미터(Skin Surface Hygrometer), 모델명: SKICON-200, 일본 IBS사 제품)를 이용하여 측정하였고, 그 결과는 하기 표 5에 나타내었다. 여기서 사용된 수치의 단위는 ㎲이다.In order to determine the water retention ability of the vesicle lipids, the following experiment was conducted twice a day for 20 months on the male and female adults with dry hands. First, the skin moisture content measuring device (skin skin hygrometer), model name: SKICON-200, Japan IBS Product) and the results are shown in Table 5 below. The unit of measure used here is ㎲.

수분 보유능 측정 결과Moisture holding capacity measurement result 구 분division 도포전 측정값 평균Average of measured values before application 도포후 측정값 평균Average of measured values after application 화장수toilet water 실시예 1Example 1 2.52.5 19.019.0 실시예 2Example 2 2.42.4 22.022.0 비교예 1Comparative Example 1 2.62.6 18.018.0 영양유액Nutrition 실시예 3Example 3 2.52.5 18.218.2 실시예 4Example 4 2.52.5 23.023.0 비교예 2Comparative Example 2 2.62.6 13.413.4 크림cream 실시예 5Example 5 2.42.4 24.424.4 실시예 6Example 6 2.42.4 26.526.5 비교예 3Comparative Example 3 2.52.5 17.717.7 팩pack 실시예 7Example 7 2.62.6 8.38.3 실시예 8Example 8 2.42.4 8.58.5 비교예 4Comparative Example 4 2.52.5 7.07.0

상기 표 5에서 알 수 있듯이, 건조한 피부에 대하여 본 발명의 실시예 1 내지 8의 화장료를 도포한 후에 수분 보유능이 증가되었다. 또한, 실시예 1 내지 8의 경우 비교예 1 내지 4의 화장료를 도포한 것에 비해 수분 보유능이 더욱 증가되어 피부보습 및 유연효과가 우수함을 알 수 있다.As can be seen in Table 5, after applying the cosmetic of Examples 1 to 8 of the present invention on dry skin, the water retention capacity was increased. In addition, in the case of Examples 1 to 8 it can be seen that the moisture retention ability is further increased compared to the cosmetics of Comparative Examples 1 to 4 it is excellent in the skin moisturizing and softening effect.

실험예 2Experimental Example 2

(피.아크네스에 대한 살균력 실험)(Sterilization test on P. acnes)

실시예 1 내지 8 및 비교예 1 내지 4에서 제조한 화장료 조성물의 피.아크네스에 대한 항균력을 평가하기 위하여, 멸균된 비에취아이(BHI) 액체 배지가 10 ml 담긴 시험관에 피.아크네스를 접종하여 2일간 혐기성 배양기에서 배양하고, 피.아크네스의 집락수(N1)를 측정하였으며, 실시예 및 비교예의 10% 화장료 수용액을 상기 시험관에 1 g을 첨가하여 1일간 혐기성 배양기에서 배양시킨 후 집락수(N2)를 측정하였다. 상기에 의하여 구하여진 집락수를 이용하여 피.아크네스에 대한 살균력을 평가하였다. 피.아크네스의 살균율은 90% 이상의 결과가 있어야 살균효과가있는 것으로 판단한다. 피.아크네스에 대한 살균력 실험결과는 하기 표 6에 나타내었다.In order to evaluate the antimicrobial activity of P. acnes of the cosmetic compositions prepared in Examples 1 to 8 and Comparative Examples 1 to 4, P. acne was added to a test tube containing 10 ml of sterile BHI liquid medium. Inoculated and incubated in an anaerobic incubator for 2 days, and the colony number (N1) of P. acnes was measured, and 1 g of 10% cosmetic solution of Examples and Comparative Examples was added to the test tube and then incubated in an anaerobic incubator for 1 day. Colony number N2 was measured. The bactericidal power against P. acnes was evaluated using the colony number obtained above. The bactericidal rate of P. acnes should be more than 90% in order to be effective. The bactericidal test results for P. acnes are shown in Table 6 below.

구 분division 처음 배양한피.아크네스의 수(N1)First incubated blood, acnes number (N1) 화장료를 가한 3일 후의피.아크네스의 수(N2)Blood after 3 days of applying cosmetics.Number of acnes (N2) 살균율(%)Sterilization Rate (%) 화장수toilet water 실시예 1Example 1 1.50×108 1.50 × 10 8 1.50×104 1.50 × 10 4 99.9999.99 실시예 2Example 2 1.50×108 1.50 × 10 8 7.5×104 7.5 × 10 4 99.9599.95 비교예 1Comparative Example 1 1.50×108 1.50 × 10 8 1.8×107 1.8 × 10 7 8888 영양유액Nutrition 실시예 3Example 3 1.30×108 1.30 × 10 8 6.5×106 6.5 × 10 6 9595 실시예 4Example 4 1.30×108 1.30 × 10 8 9.1×106 9.1 × 10 6 9393 비교예 2Comparative Example 2 1.30×108 1.30 × 10 8 3.25×106 3.25 × 10 6 7575 크림cream 실시예 5Example 5 1.00×108 1.00 × 10 8 9.0×106 9.0 × 10 6 9191 실시예 6Example 6 1.00×108 1.00 × 10 8 9.5×106 9.5 × 10 6 90.590.5 비교예 3Comparative Example 3 1.00×108 1.00 × 10 8 3.5×107 3.5 × 10 7 6565 팩pack 실시예 7Example 7 1.70×108 1.70 × 10 8 1.36×107 1.36 × 10 7 9292 실시예 8Example 8 1.70×108 1.70 × 10 8 1.19×107 1.19 × 10 7 9393 비교예 4Comparative Example 4 1.70×108 1.70 × 10 8 4.93×107 4.93 × 10 7 7171

상기 표 6에서 보면, 실시예 1 내지 8의 경우 비교예 1 내지 4와 비교하여 피.아크네스에 대한 살균율이 월등히 우수함을 알 수 있다. 특히, 실시예 1 및 2의 경우 피.아크레스에 대한 살균율이 99.99 및 99.95%로 매우 우수하였다.In Table 6, it can be seen that in Examples 1 to 8, the sterilization rate for P. acnes was much better than that of Comparative Examples 1 to 4. In particular, in Examples 1 and 2, the sterilization rates for P. acres were 99.99 and 99.95%.

실험예 3Experimental Example 3

(코리네박테리움 제로시스에 대한 살균력 실험)(Sterilization test for Corynebacterium zerosis)

실시예 1 내지 8 및 비교예 1 내지 4에서 제조한 화장료 조성물의 코리네박테리움 제로시스에 대한 항균력을 평가하기 위하여, 트윈-80이 0.1% 첨가된 멸균된 비에치아이(BHI) 액체 배지가 10 ml 담긴 시험관에 코리네박테리움 제로시스를 접종하여 2일간 호기성 배양기에서 배양하고 코리네박테리움 제로시스의 집락수(N1)를 측정하였으며, 실시예 및 비교예의 화장료를 상기 시험관에 1 g을 첨가하여 1일간 혐기성 배양기에서 배양시킨 후 집락수(N2)를 측정하였다. 상기에 의하여 구하여진 집락수를 이용하여 코리네박테리움 제로시스에 대한 살균력을 평가하였다. 코리네박테리움 제로시스의 살균율은 90% 이상의 결과가 있어야 살균효과가 있는 것으로 판단한다. 코리네박테리움 제로시스에 대한 살균력 실험결과는 하기 표 7에 나타내었다.In order to evaluate the antimicrobial activity against Corynebacterium zerosis of the cosmetic compositions prepared in Examples 1 to 8 and Comparative Examples 1 to 4, sterilized BH liquid medium containing 0.1% of Tween-80 was added. Corynebacterium zerosis was inoculated into a test tube containing 10 ml, and cultured in an aerobic incubator for 2 days, and the colony count (N1) of Corynebacterium zerosis was measured, and 1 g of the cosmetics of Examples and Comparative Examples were added to the test tube. Colonies (N2) were measured after 1 day of incubation in an anaerobic incubator. The bactericidal activity against Corynebacterium zerosis was evaluated using the colony numbers obtained above. The sterilization rate of Corynebacterium zerosis should be more than 90% in order to be effective. Results of bactericidal power for Corynebacterium zerosis are shown in Table 7 below.

구 분division 처음 배양한코리네박테리움 제로시스의 수(N1)Number of First-Generated Corynebacterium Zerosis (N1) 화장료를 가한 3일 후의코리네박테리움 제로시스의 수(N2)Number of Corynebacterium zerosis 3 days after applying cosmetics (N2) 살균율(%)Sterilization Rate (%) 화장수toilet water 실시예 1Example 1 2.0×106 2.0 × 10 6 2.0×104 2.0 × 10 4 9999 실시예 2Example 2 2.0×106 2.0 × 10 6 4.0×104 4.0 × 10 4 9898 비교예 1Comparative Example 1 2.0×106 2.0 × 10 6 3.0×105 3.0 × 10 5 8585 영양유액Nutrition 실시예 3Example 3 4.0×106 4.0 × 10 6 2.7×105 2.7 × 10 5 9393 실시예 4Example 4 4.0×106 4.0 × 10 6 2.4×105 2.4 × 10 5 9494 비교예 2Comparative Example 2 4.0×106 4.0 × 10 6 9.2×105 9.2 × 10 5 7777 크림cream 실시예 5Example 5 3.2×106 3.2 × 10 6 3.2×105 3.2 × 10 5 9090 실시예 6Example 6 3.2×106 3.2 × 10 6 2.88×105 2.88 × 10 5 9191 비교예 3Comparative Example 3 3.2×106 3.2 × 10 6 1.06×106 1.06 × 10 6 6767 팩pack 실시예 7Example 7 5.6×106 5.6 × 10 6 4.48×105 4.48 × 10 5 9292 실시예 8Example 8 5.6×106 5.6 × 10 6 3.92×105 3.92 × 10 5 9393 비교예 4Comparative Example 4 5.6×106 5.6 × 10 6 1.68×106 1.68 × 10 6 7070

상기 표 7에서 보면, 실시예 1 내지 8의 경우 비교예 1 내지 4와 비교하여 코리네박테리움 제로시스에 대한 살균율이 월등히 우수함을 알 수 있다. 특히, 실시예 1의 경우 코리네박테리움 제로시스에 대한 살균율이 99로 매우 우수하였다.In Table 7, it can be seen that in Examples 1 to 8, compared to Comparative Examples 1 to 4, the sterilization rate for Corynebacterium zerosis is excellent. In particular, in Example 1, the sterilization rate against Corynebacterium zerosis was 99, which was very good.

이상에서 살펴본 바와 같이, 본 발명은 천연 미생물을 배양하여 생산된 소포로리피드를 생물계면활성제로 포함하여 여드름균과 액취균에 대한 살균효과, 피부보습효과, 및 사용감촉과 피부유연 효과가 매우 우수한 화장료 조성물을 제공할 수 있다.As described above, the present invention includes a microorganism produced by culturing natural microorganisms as a biosurfactant, the bactericidal effect, acne moisturizing effect on the acne and liquid odor bacteria, and the feeling of use and skin softness is very excellent Cosmetic compositions can be provided.

Claims (3)

캔디다 봄비오콜라(Candida bombiocola)(ATCC 22214)로부터 생산된 소포로리피드를 유효성분으로 포함하는 화장료 조성물. Candida bombiocola ( Candida bombiocola ) (ATCC 22214) comprising a cosmetic composition comprising a vesicle lipids as an active ingredient. 제 1 항에 있어서,The method of claim 1, 상기 소포로리피드의 함량이 0.01 내지 10 중량%인 것을 특징으로 하는 화장료 조성물.Cosmetic composition, characterized in that the content of the phosphorelipid is 0.01 to 10% by weight. 제 1 항에 있어서,The method of claim 1, 상기 화장료 조성물의 제형이 화장수, 영양유액, 크림 또는 화장수인 것을 특징으로 하는 화장료 조성물.Cosmetic composition, characterized in that the formulation of the cosmetic composition is a lotion, nutrient fluid, cream or lotion.
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